Digital Commons Network^™

Wee1 And Cell Size Control In Fission Yeast By The Protein Kinase Cdr2, Rachel Berg-Murante

Dartmouth College Ph.D Dissertations

The mechanisms that govern cell size have long been topics of study in the field of cell biology. In eukaryotic cells this size control is tied to checkpoints, a set threshold of minimum necessary growth linked to cyclin dependent kinase activity regulation. In the fission yeast Schizosaccharomyces pombe, the Cdk1 regulatory network is conserved, and G2/M represents the major size checkpoint. Prior to mitosis, Cdk1 is inhibited by phosphorylation applied by Wee1 during G2 phase. Once S. pombe cells have satisfied the size checkpoint, Cdk1 is activated through dephosphorylation by Cdc25. Wee1 is a dose-dependent regulator of mitotic entry …

Resilience: Understanding The Role Of Coping In A Racialized Society Among Black Women, Danielle Pendarvis

ETD Collection

The present study explored the intricate relationships between race-based stress, coping strategies (Emotion-focused, Problem-focused, Avoidant coping), the Black Superwoman Schema, resilience, and health outcomes among Black women. Employing a mixed-methods design, an online survey (N = 97) and in-depth semi-structured interviews (N = 10) were conducted, with five transcripts selected for detailed thematic analysis. Quantitative results revealed significant relationships between race-based stress, coping strategies, and adherence to the Black Superwoman Schema. Specifically, race-based stress predicted lower levels of general health, while adherence to the Black Superwoman Schema was associated with diverse coping strategies and higher levels of race-based resilience. Moderation …

Evaluating Injury Risk Caused By Potential Drift Of Florpyrauxifen-Benzyl Or Quinclorac In Corn, Amar Godar, Jason K. Norsworthy, L. Barber

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Of relevance to the off-target movement of herbicides in Arkansas is the drift of rice herbicides in the state’s agricultural landscape, where aerial application of herbicides is common, raising the prima facie question of the risk of damage to other sensitive crops. The aim of this study was to evaluate the response of corn to postemergence applications of florpyrauxifen-benzyl or quinclorac, which were applied directly to the crop at low rates (0.78 or 1.56 to 12.5% of the normal use rate for labeled crops). This was investigated through three site-year field experiments conducted in Fayetteville (2020 and 2021) and Tillar …

Serotonin Receptor-Mediated Vasorelaxation Occurs Primarily Through 5-Ht4 Activation In Bovine Lateral Saphenous Vein, Ronald J. Trotta, David L. Harmon, James L. Klotz

Animal and Food Sciences Faculty Publications

To better understand mechanisms of serotonin- (5-HT) mediated vasorelaxation, isolated lateral saphenous veins from cattle were assessed for vasoactivity using myography in response to increasing concentrations of 5-HT or selective 5-HT receptor agonists. Vessels were pre-contracted with 1 × 10⁻⁴ M phenylephrine and exposed to increasing concentrations of 5-HT or 5-HT receptor agonists that were selective for 5-HT_1B, 5-HT_2B, 5-HT₄, and 5-HT₇. Vasoactive response data were normalized as a percentage of the maximum contractile response induced by the phenylephrine pre-contraction. At 1 × 10⁻⁷ M 5-HT, a relaxation was observed …

Spatial And Abiotic Effects Of Urbanization On Small Mammal Communities, Bri Casement

Master's Theses

Urbanization, the phenomenon of increasing human population density and artificial structures, is known to decrease the richness, abundance, and diversity of native wildlife populations, fragment natural areas, and increase human and human-commensal animal interactions with wildlife. Dynamics of species persistence in habitat fragments is often understood in the framework of island biogeography theory (IBT), with habitat fragments being analogous to oceanic islands. Despite the conceptual similarities between oceanic islands in IBT and urbanized habitat fragments (fragment size, fragment age, and distance to immigration sources), there are important differences. In urban fragments, the matrix is not completely impassible, habitats within fragments …

Characterization And Evaluation Of Bacteriophages Φpa-C And Φpa-Tc Infecting Pseudomonas Aeruginosa, Joanne Harrison

Master's Theses

Pseudomonas aeruginosa are prevalent opportunistic bacterial pathogens that cause nosocomial infections in at-risk individuals. The rise of multidrug-resistant P. aeruginosa poses an immense threat to hospitals. Additionally, P. aeruginosa are common food spoilage organisms that contribute to food waste. Alternative effective treatment methods are needed to improve public health and reduce food waste. Recent studies have shown that bacteriophages, viruses that only kill host-specific bacteria, are promising alternatives. This study characterizes two lytic P. aeruginosa phages, ΦPa-C and ΦPa-Tc, and evaluates their efficacy against P. aeruginosa in two model food systems. The morphology, genome, structural proteins, host range, growth kinetics, …

Volume 33, Number 2, Summer/Fall 2024, Office Of Communications, Illinois Wesleyan University

Illinois Wesleyan University Magazine, 2018-present

VOLUME 33 | NUMBER 2 | Summer/Fall 2024

ON OUR COVER:

President Sheahon Zenger poses for a portrait in the Bates & Merwin Reading Room on the fourth floor of The Ames Library. The room hosts a collection of award-winning books in honor of IWU's former president Minor Myers jr.

18 Discoveing Home, As a career university administrator with a long record of success and optimism for the future of liberal arts, Sheahon Zenger has been appointed the 21st president of Illinois Wesleyan University.

22 Browadway Reunion, More than a decade after last performing together at IWU, Bry Parham '07 …

Examining Risk Factors For Early-Onset Breast Cancer, Jennifer Grace Taylor

Theses and Dissertations

Introduction: There have been great advancements in screening and in the identifying of risk factors associated with breast cancer, however, there is a lack of information of these factors in women with breast cancer occurring under the age of 45, known as early-onset breast cancer (EOBC). EOBC is often found at later stages, negatively impacting young women’s careers, family planning, and mental health. Additionally, because of the health disparities that exist within the U.S. health care system, women belonging to racial and ethnic groups, specifically young Black women, tend to have poorer outcomes and higher mortality rates of EOBC compared …

"Ain’T No Slump...I Just Ain’T Hittin’": Investigating Psychological Momentum And Loss Aversion In Major League Baseball, Josephine Matalone

Theses and Dissertations

Throughout the history of America’s pastime, it has long been concluded among fans, athletes, and coaches alike that momentum is a key element necessary should one want to win when it counts. However, this issue of “hot hand” existence in the context of baseball has been largely glazed over since the canon of literature on the topic was initiated, and even more so has the concept of a “cold hand.” This study seeks to make a novel contribution by exploring streaks in professional baseball. Using 10-years’ worth of regular season MLB data between 2012 and 2021, a comparative analysis of …

Antioxidant And Anti-Inflammatory Potential Of Engineered Ceria Nanoparticles In The Management Of Alzheimer's Disease, Shanshan Shi

Theses and Dissertations

Alzheimer's disease (AD) is a devastating neurodegenerative disorder that affects millions worldwide. Unfortunately, there are currently no effective therapies available to prevent or cure the disease. While current therapies aim to change AD progression, there is a growing need for novel therapeutic strategies that can effectively penetrate the blood-brain barrier (BBB) to target the underlying pathology of the disease. The BBB is a highly selective semipermeable barrier that separates circulating blood from the brain. It plays a crucial role in amyloid-β (Aβ) homeostasis and clearance dynamics, as Aβ accumulation is a key factor in the pathogenesis of AD. Aβ aggregation …

“In My Blood”: External Factors For International Stem Postdoctoral Scholars’ Career Decisions, Kathryn J. Watson, Sylvia L. Mendez

Journal of Global Education and Research

This instrumental case study (Stake, 1995) explores the external factors that influence international science, technology, engineering, and mathematics (STEM) postdoctoral scholars in the United States to pursue a career in STEM. Interviews with 20 international STEM postdoctoral scholars were analyzed deductively to shed light on their unique backgrounds and experiences. Three themes emerged: (a) parents were highly encouraging, (b) a love of science was nurtured in school, and (c) they were eager to engage in and promote scientific innovation. These findings illustrate the ways in which family, schools, and community influence the STEM career trajectories of international postdoctoral scholars.

Meta-Narrative Review Of Gender Portrayal In Disney Movies For Young Children And Its Pedagogical Implications, Xuan Jiang, Linlin Zhang, Diana Rivero, Brittany Torres

Journal of Global Education and Research

Many of Disney movies have been criticized for perpetuating traditional gender stereotypes which constrain opportunities for children at large. Its recent characters have also incurred heated discussions on gender portrayal. Moreover, stereotypes of gender roles, developed early on, can exert an immediate impact on individuals’ behaviors and utterances and a long-term impact on individuals’ perceived options academically, professionally, personally, and socially. Recognizing the significance of this matter, this paper employed a meta-narrative review to collect and conceptually and empirically synthesize previous literature on the impact of Disney movies on young children’s gender awareness. Through an academic database search, 49 articles …

“Finally Somebody Wants To Hear Us”: Soviet Women And Political Violence In Svetlana Alexievich’S Narratives, Liubov Kartashova

Theses and Dissertations

This dissertation explores the emotional and social impact of significant Soviet historical events, including World War II, the Soviet-Afghan War, and the Chornobyl disaster, on collective memory. Svetlana Alexievich, a Belarusian Nobel Prize laureate, employs a polyphonic narrative technique to challenge official histories of these events and foreground the voices of ordinary people, particularly women. Focusing on her works – The Unwomanly Face of War, Zinky Boys, and Chernobyl Prayer – the study delves into the gendered experiences and narratives of women involved in these events. Integrating feminist scholarship on international relations and security, this research analyzes women’s roles in …

Improving Drug Repurposing Success: Integrating Large Database Claims Analysis In Drug Discovery, Joseph Magagnoli

Theses and Dissertations

Drug repurposing has emerged as an innovative strategy to identify new therapeutic uses for existing medications. However, with few notable exceptions, repurposing existing drugs has limited overall success, often failing in randomized controlled trials (RCTs) due to inadequate treatment efficacy in new clinical settings. To improve success rates, I propose a framework titled REMIDY: Retrospective and Experimental Methods Integration for Drug DiscoverY. The REMIDY framework incorporates retrospective claims analysis into the drug discovery process. I demonstrate that retrospective analysis can leverage extensive administrative and clinical data to address translational gaps between pre-clinical experimental models and RCTs. For example, pre-clinical experimental …

Humanizing Healthcare: Supporting Lgbtq+ Healthcare Professionals And Trainees, Atticus Wolfe

Theses and Dissertations

The public perception of the healthcare profession has been shifting in recent years largely attributed to the rise of scientific and medical skepticism impacting perceptions of the value of healthcare professionals and care itself (Goddard & Patel 2021). While healthcare professions remain associated with higher public value, healthcare professionals and trainees are expected to adhere to rigid expectations of medical professionalism that may contradict other aspects of their identities. LGBTQ+ identity in particular may pose conflicts to the expectations of healthcare professionalism, contributing to lower self-perceived value for LGBTQ+ healthcare professionals and trainees compared to non-LGBTQ+ healthcare professionals and trainees …

Leaf Development In The Reproductive Phase Of Arabidopsis: Investigating The Mechanics Of Cauline Leaf Identity And Bract Suppression, Darren Paul Manuela

Theses and Dissertations

Leaves in the reproductive phase subtend inflorescences or flowers and exhibit diverse morphological modifications specialized for reproductive success, such as attracting pollinators, protecting flowers/fruit, and photosynthesis. Using the model organism Arabidopsis, we investigated the development of these specialized leaves. In Arabidopsis, uniquely shaped cauline leaves subtend inflorescences, protect young floral buds and exhibit increased photosynthetic activity crucial for seed production. As form affects function, we analyzed gene expression patterns by RT-qPCR and GUS reporter genes in cauline leaves and examined mutant phenotypes with defective cauline leaf morphology. Our results reveal that redundant miR156-targeted SPLs function to promote cauline leaf identity …

Exploring The Impact Of Neonatal Transport On The Health Of Black Preterm Infants And Psychosocial Challenges Faced By Parents In Relation To Social Determinants Of Socioeconomic And Racial Inequities, Karen Faye Warren

Theses and Dissertations

Neonatal transport is a unique aspect of preterm birth. This descriptive, exploratory dissertation study used a multiple-case study design, and mixed methods approach to describe the trajectory of Black preterm outborn infants and their mothers from birth, through transport to a referral center through NICU hospitalization until discharge, for a detailed analysis of each case. The specific aims were 1) Describe each infant's transport trajectory, including baseline characteristics of the community hospital of birth (size, level of care, birthing resources), transport characteristics (mode, distance, personnel), and infant health from birth to arrival of regional referral neonatal intensive care unit; 2) …

A Careful Examination Of The Parameters Impacting The Study Of Plant/Bacteria Interactions, Caroline Victoria Royals

Theses and Dissertations

Our laboratory has been studying how different Caulobacter strains impact Arabidopsis plant growth. While reading papers published by other laboratories, I noticed inconsistencies in the methods used by different plant/bacteria interaction studies. For example, a variety of different variables have been used for defining a bacterium as Plant Growth Promoting (PGP). To establish a baseline, I conducted longitudinal studies that examined the impact of bacteria on all phases of a plant’s life. I found that individual Caulobacter wild type strains impact different aspects of plant growth. In addition, I continuously encountered problems with within and between pot variation in plant …

Evidence Of Lactobacillus Strains Shared Between The Female Urinary And Vaginal Microbiota, Haley Atkins, Baani Sabharwal, Leah Boger, Natalie Stegman, Alexander Kula, Alan J. Wolfe, Swarnali Banerjee, Catherine Putonti

Biology: Faculty Publications and Other Works

Lactobacillus species are common inhabitants of the ‘healthy’ female urinary and vaginal communities, often associated with a lack of symptoms in both anatomical sites. Given identification by prior studies of similar bacterial species in both communities, it has been hypothesized that the two microbiotas are in fact connected. Here, we carried out whole-genome sequencing of 49 Lactobacillus strains, including 16 paired urogenital samples from the same participant. These strains represent five different Lactobacillus species: L. crispatus, L. gasseri, L. iners, L. jensenii, and L. paragasseri. Average nucleotide identity (ANI), alignment, single-nucleotide polymorphism (SNP), and CRISPR comparisons between strains from the …

Draft Genome Sequences For A Staphylococcus Aureus And A Staphylococcus Haemolyticus Isolate From Nasal Swab Samples From Healthy Females, Sandra Jablonska, Hannah Brown, Helen Appleberry, Catherine Putonti, Alex Kula

Biology: Faculty Publications and Other Works

Staphylococcus aureus and Staphylococcus haemolyticus are common members of the human microbiota, but they are also opportunistic pathogens. To identify antibiotic resistance in healthy individuals, we present the genome sequences of S. aureus 139 N-1 and S. haemolyticus 173 N-3, both isolated from nasal swab samples from asymptomatic female participants.

Insights Into Personalized Care Strategies For Wilms Tumor: A Narrative Literature Review, Salma Karam, Ahmad Gebreil, Ahmed Alksas, Hossam Magdy Balaha, Ashraf Khalil, Mohammed Ghazal, Sohail Contractor, Ayman El-Baz

All Works

Wilms tumor (WT), or nephroblastoma, is the predominant renal malignancy in the pediatric population. This narrative review explores the evolution of personalized care strategies for WT, synthesizing critical developments in molecular diagnostics and treatment approaches to enhance patient-specific outcomes. We surveyed recent literature from the last five years, focusing on high-impact research across major databases such as PubMed, Scopus, and Web of Science. Diagnostic advancements, including liquid biopsies and diffusion-weighted MRI, have improved early detection precision. The prognostic significance of genetic markers, particularly WT1 mutations and miRNA profiles, is discussed. Novel predictive tools integrating genetic and clinical data to anticipate …

Archeota Spring/ Summer 2024, Emily Mizokami, Ali Phelps, Celina Lozano, Araceli Argueta, Chelsey Abbott, Danielle Bonsignore, Sarah Lewis

Archeota

Archeota is a platform for SJSU iSchool students to contribute to the archival conversation. It is written BY students, FOR students. It provides substantive content on archival concerns and issues and promotes professional development in the field of archival studies. Archeota upholds the core values of the archival profession.

Contents:

Reslience and Pride at the LLACE by Emily Mizokami

Black Americana at the Museum of Ventura County by Ali Phelps

Interview with Archivist Maria Carillo Colato by Celina Lozano

Gabriel Vidal's Central American Music Archive by Araceli Argueta

Preserving Peanuts by Danielle Bonsignore

Interference Archives by Chelsey Abbott

Preservation Workshop …

Radiation Dose Attenuation Using Radpad In Cath Lab For Primary And Secondary Operators – Radar-Cath Study, Sandeepan Saha, Aditya Kapoor, Kamlesh Raut, Arpita Katheria, Harshit Khare, Ankit Sahu, Roopali Khanna, Sudeep Kumar, Naveen Garg, Satyendra Tewari

Faculty, Staff and Student Publications

BACKGROUND: Radiation injury is an important concern for interventional cardiologists and needs to be addressed. RADPAD is a radiation protection drape that has been shown to reduce the radiation exposure of the primary operator (PO). While Indian data on radiation exposure of the PO in the cath lab are scarce, the exposure of the secondary operator (SO) is even less well studied.

AIMS: The aim of this study was to evaluate the efficacy of RADPAD drapes in reducing radiation doses in the cath lab for the primary as well as the secondary operator.

METHODS: A total of 160 patients (40 …

Eus-Guided Hydrogel Injection To Separate Pancreatic Head Carcinoma From Duodenum For Enhanced Radiotherapy: Multi-Site Feasibility Study, Manoop S Bhutani, Amol K Narang, Kai Ding, Brenna Casey, Kumar Krishnan, Eugene J Koay, Theodore S Hong, Joseph M Herman, Kristen H Griffin, Eun Ji Shin

Faculty, Staff and Student Publications

Background and study aims The proximity of a pancreas head tumor to the duodenum often limits delivery of an ablative dose of radiation therapy. This study evaluated the feasibility and safety of using an injectable polyethylene glycol (PEG) hydrogel between the head of the pancreas and duodenum.

Patients and methods In a multi-site feasibility cohort study of patients with localized pancreatic cancer, PEG hydrogel was injected under endoscopic ultrasound guidance to temporarily position the duodenum away from the pancreas. Procedure characteristics were recorded, including hydrogel volume and space created. Patients were monitored for adverse events (AEs) and radiotherapy toxicity.

Results …

Sequencing Of Kaposi’S Sarcoma Herpesvirus (Kshv) Genomes From Persons Of Diverse Ethnicities And Provenances With Kshv-Associated Diseases Demonstrate Multiple Infections, Novel Polymorphisms, And Low Intra-Host Variance, Vickie A Marshall, Elena M Cornejo Castro, Charles A Goodman, Nazzarena Labo, Isabella Liu, Nicholas C Fisher, Kyle N Moore, Ananthakrishnan Nair, Taina Immonen, Brandon F Keele, Mark N Polizzotto, Thomas S Uldrick, Yunxiang Mu, Tanuja Saswat, Laurie T Krug, Kevin M Mcbride, Kathryn Lurain, Ramya Ramaswami, Robert Yarchoan, Denise Whitby

Faculty, Staff and Student Publications

Recently published near full-length KSHV genomes from a Cameroon Kaposi sarcoma case-control study showed strong evidence of viral recombination and mixed infections, but no sequence variations associated with disease. Using the same methodology, an additional 102 KSHV genomes from 76 individuals with KSHV-associated diseases have been sequenced. Diagnoses comprise all KSHV-associated diseases (KAD): Kaposi sarcoma (KS), primary effusion lymphoma (PEL), KSHV-associated large cell lymphoma (KSHV-LCL), a type of multicentric Castleman disease (KSHV-MCD), and KSHV inflammatory cytokine syndrome (KICS). Participants originated from 22 different countries, providing the opportunity to obtain new near full-length sequences of a wide diversity of KSHV genomes. …

Ccr2+ Monocytes Promote White Matter Injury And Cognitive Dysfunction After Myocardial Infarction, Edward B Thorp, Mallory Filipp, Maria Dima, Chunfeng Tan, Matthew Feinstein, Brian Popko, Matthew Deberge

Faculty, Staff and Student Publications

Survivors of myocardial infarction are at increased risk for vascular dementia. Neuroinflammation has been implicated in the pathogenesis of vascular dementia, yet little is known about the cellular and molecular mediators of neuroinflammation after myocardial infarction. Using a mouse model of myocardial infarction coupled with flow cytometric analyses and immunohistochemistry, we discovered increased monocyte abundance in the brain after myocardial infarction, which was associated with increases in brain-resident perivascular macrophages and microglia. Myeloid cell recruitment and activation was also observed in post-mortem brains of humans that died after myocardial infarction. Spatial and single cell transcriptomic profiling of brain-resident myeloid cells …

The Time-Dependent Association Between Irritable Bowel Syndrome And All-Cause And Cause-Specific Mortality: A Prospective Cohort Study Within The Uk Biobank, Fangyu Li, Yukiko Yano, Lola Étiévant, Carrie R Daniel, Shreela V Sharma, Eric L Brown, Ruosha Li, Erikka Loftfield, Qing Lan, Rashmi Sinha, Baharak Moshiree, Maki Inoue-Choi, Emily Vogtmann

Faculty, Staff and Student Publications

Introduction: Irritable bowel syndrome (IBS) is one of the most common functional gastrointestinal disorders, but few studies have evaluated mortality risks among individuals with IBS. We explored the association between IBS and all-cause and cause-specific mortality in the UK Biobank.

Methods: We included 502,369 participants from the UK Biobank with mortality data through 2022. IBS was defined using baseline self-report and linkage to primary care or hospital admission data. We estimated hazard ratios (HRs) and 95% confidence intervals (CIs) for all-cause and cause-specific mortality using multivariable Cox proportional hazards regression models within partitioned follow-up time categories (0-5, >5-10, and >10 …

Reproducibility Of Quantitative Myocardial Perfusion And Coronary Flow Capacity By Positron Emission Tomography: 3d Digital Silicon Photomultiplier Solid State Vs Legacy 2d Analogue Systems For Clinical Practice And Trials, Amanda Roby, Lindsey Harmon, Kelly Sander, Linh Bui, Danai Kitkungvan, Monica Patel, Jagat Narula, Nils P Johnson, K Lance Gould

Faculty, Staff and Student Publications

Aims: Quantitative rest-stress myocardial perfusion in millilitres per minute per gram among multiple 2D and 3D positron emission tomography-computed tomography (PET-CT) scanners is essential for personalized cardiac management and clinical trials. Accordingly, this study reports the accuracy and precision of quantitative rest-stress millilitres per minute per gram and coronary flow capacity among 2D and two different digital 3D silicon photomultiplier (SiPM) PET-CT scanners for quantifying the severity of coronary pathophysiology for clinical trials or guiding interventions vs. medical treatment.

Methods and results: One hundred seventy-one participants underwent 748 paired serial rest or stress PET perfusion imaging in the same person …

Use Of Ipsc-Derived Smooth Muscle Cells To Model Physiology And Pathology, Callie S Kwartler, Jose Emiliano Esparza Pinelo

Faculty, Staff and Student Publications

The implementation of human induced pluripotent stem cell (hiPSC) models has introduced an additional tool for identifying molecular mechanisms of disease that complement animal models. Patient-derived or CRISPR/Cas9-edited induced pluripotent stem cells differentiated into smooth muscle cells (SMCs) have been leveraged to discover novel mechanisms, screen potential therapeutic strategies, and model in vivo development. The field has evolved over almost 15 years of research using hiPSC-SMCs and has made significant strides toward overcoming initial challenges such as the lineage specificity of SMC phenotypes. However, challenges both specific (eg, the lack of specific markers to thoroughly validate hiPSC-SMCs) and general (eg, …

Expanded-Access Use Of Elamipretide In A Patient With Membrane Protein-Associated Neurodegeneration, Jorge Patino, Anna Haertling Clearman, Lindsey Miller, Mary Kay Koenig

Faculty, Staff and Student Publications

This case report presents a progressively declining 17-year-old patient with membrane protein-associated neurodegeneration who demonstrated symptomatic improvements in her dysarthria, dysphagia, and gait, and objective improvements in her 6-minute walk test and 5 times sit-to-stand test during elamipretide treatment.