Digital Commons Network^™

Development Of Vla4 And Cxcr4 Antagonists For The Mobilization Of Hematopoietic Stem And Progenitor Cells, Peter G Ruminski, Michael P Rettig, John F Dipersio

2020-Current year OA Pubs

The treatment of patients diagnosed with hematologic malignancies typically includes hematopoietic stem cell transplantation (HSCT) as part of a therapeutic standard of care. The primary graft source of hematopoietic stem and progenitor cells (HSPCs) for HSCT is mobilized from the bone marrow into the peripheral blood of allogeneic donors or patients. More recently, these mobilized HSPCs have also been the source for gene editing strategies to treat diseases such as sickle-cell anemia. For a HSCT to be successful, it requires the infusion of a sufficient number of HSPCs that are capable of adequate homing to the bone marrow niche and …

What Are The Most Effective Non-Pharmacologic Practices For The Management Of Primary Insomnia In Adults?, Alexis Myhre

Theses and Graduate Projects

Objective: Primary insomnia is a mental health condition in which individuals struggle with falling asleep, staying asleep or early morning awakenings. As there are many treatments available that claim to help those with primary insomnia, this review looks specifically at therapy, exercise and acupuncture as management strategies.

Methods: This is a review of 15 peer-reviewed, randomized controlled trials completed in 2018 or later with one additional study completed in 2010. Also, there are four non primary insomnia based studies used to support the efficacy of alternative therapies to cognitive behavioral therapy (CBT). All the studies used in this review come …

The Efficacy Of Cannabis As A Substitute Addiction For Opiates, Clark Furlong

Theses and Graduate Projects

Background: Approaches to opiate addiction focus on abstinence and/or a harm reduction model that often utilizes substitute medications. Methadone and suboxone are the most prescribed drugs for opiate maintenance therapy. The long-term side effect profile and health consequences are undesirable. Research is looking for healthier alternatives.

Purpose: Opiate maintenance therapy participants have a mortality rate greater than twelve times that of the normal population, we seek to discover if cannabis can be a useful/safer alternative.

Methods: Following a comprehensive literature review a 7-question online survey was designed with SurveyMonkey to gauge current Augsburg students’ perception of cannabis as an alternative …

Attraction Is Altered Via Modulation Of The Medial Prefrontal Cortex Without Explicit Knowledge, Samantha Zorns, Claudia Sierzputowski, Sydney Ash, Molly Skowron, Anthony Minervini, Adriana Lavarco, Matthew Pardillo, Julian Paul Keenan

Department of Biology Faculty Scholarship and Creative Works

Previous studies have demonstrated that brain stimulation can alter an individual's physical appearance via dysregulation of the medial prefrontal cortex (MPFC). In this study, we attempted to determine if individuals who receive repetitive transcranial magnetic stimulation (rTMS) delivered to the MPFC were rated as more attractive by others. It has been previously reported that 1 hertz (Hz) (inhibitory) TMS can alter one's facial expressions such that frontal cortex inhibition can increase expressiveness. These alterations, detected by external observation, remain below the level of awareness of the subject itself. In Phase I, subjects (N = 10) received MPFC rTMS and had …

Utilization Of Sodium Flame Synthesis For The Formation And Deposition Of Pure Metal Materials For Applications In Additive Manufacturing, Zachariah Wargel

McKelvey School of Engineering Graduate Student Theses & Dissertations

Advanced material classes, such as high temperature alloys, ceramics, and refractory materials have become a key area of focus for expansion in the realm of additive manufacturing. Factors such as high melting points, material homogeneity, and physical properties such as ductility limit the application of popular additive manufacturing methods to these material classes. By utilizing sodium flame synthesis and deposition (SFD) technology, nanoparticle materials can be synthesized via combustion in a laminar co-flow diffusion burner and impacted such that the deposits can be used to manufacture designed components. By using the applicable precursor materials of metal chlorides and elemental sodium …

Using Phylogenomics And Environmental Metabarcoding To Understand The Distribution And Evolutionary Histories Of Amoebae, Robert E. Jones

Theses and Dissertations

Protists are defined as eukaryotic organisms that are not animals, land plants, or fungi and make up the vast majority of eukaryotic diversity. Unfortunately, they are often overlooked and under sampled. Some rationales behind this are that they are often microscopic, most rarely the cause of human disease, and are often difficult to get into culture. Molecular phylogenetics has revolutionized our understanding of the evolutionary history of eukaryotes and without a clear picture of protist evolution our understanding would be severely hindered. Phylofisher is an easy-to-use phylogenomic software package, with an accompanying database and various utilities, intended for deep phylogenomic …

Evolution Of Functional Diversity In Defensive Bacterial Toxins And Parasite Infection Strategy, Logan D. Moore

Theses and Dissertations

Insects and their natural enemies are engaged in a never-ending battle called the ‘co-evolutionary arms race.’ As a part of these contentious interactions, vulnerable insects evolve natural barriers that prevent successful attacks by their natural enemies. In response, natural enemies evolve strategies that overcome these barriers. Occasionally, microbial symbionts will also participate in these relationships by assisting their insect host in defense against natural enemies or by assisting the natural enemy in subduing its prey. Alternatively, microbial symbionts may become contenders themselves in the co-evolutionary arms race by becoming reproductive parasites of their hosts. To mediate successful outcomes in these …

Performance Evaluation Of Deep Learning Object Detectors For Weed Detection And Real Time Deployment In Cotton Fields, Abdur Rahman

Theses and Dissertations

Effective weed control is crucial, especially for herbicide-resistant species. Machine vision technology, through weed detection and localization, can facilitate precise, species-specific treatments. Despite the challenges posed by unstructured field conditions and weed variability, deep learning (DL) algorithms show promise. This study evaluated thirteen DL-based weed detection models, including YOLOv5, RetinaNet, EfficientDet, Fast RCNN, and Faster RCNN, using pre-trained object detectors. RetinaNet (R101-FPN) achieved the highest accuracy with a mean average precision ([email protected]) of 79.98%, though it had longer inference times. YOLOv5n, with the fastest inference (17 ms on Google Colab) and only 1.8 million parameters, achieved a comparable 76.58% [email protected], …

Windows Into Changing Landscapes: Bioarchaeological And Geophysical Analysis Of The Grenada Yellow Fever Cemetery, M. Kathryn Mckenna

Theses and Dissertations

This project focuses on the Yellow Fever Cemetery of Grenada, Mississippi through geophysical and biological methods. A ground penetrating radar survey was conducted to investigate the landscape and burial patterns in relation to the use of the cemetery throughout high periods of mortality such as a yellow fever epidemic. Additionally, biological methods were used in the analysis of an individual recovered as part of a salvage archaeology event from the cemetery. The multifaceted approach aims to uncover more about the cemetery, landscape, and community as it has changed over time with the influence of extreme events such as the yellow …

An Evaluation Of Avian Use Of Marsh Terraces In Gulf Coastal Wetlands Of Louisiana, Madelyn Belle Mcfarland

Theses and Dissertations

Louisiana’s coastal wetlands support millions of resident and migratory birds annually but account for 80% of the nation’s coastal wetland loss. Marsh terracing is a restoration technique that constructs segmented ridges in open water areas to enhance marsh conditions. Despite widespread use, their value as avian habitat has received limited study. Using ground and aerial surveys, we evaluated avian use of marsh terraces across 24 paired sites (marsh terrace and non-terrace sites) in coastal Louisiana. Avian surveys focused on breeding secretive marsh birds (SMB) and wintering waterfowl. Results indicate that presence or absence of marsh terraces influenced numbers of ducks …

Predictions Of Thermo-Fluid Transport Over A Poultry Incubation Period Using High Performance Computing, Melvy Fernandes

Theses and Dissertations

The quality of hatchery conditions significantly impacts the hatchling health. An insight into the spatiotemporal distribution of environmental factors like temperature, ventilation, humidity, and CO2 within an incubator can be the key to reduce the pathogen spread. The objective of this study is to develop an efficient computational fluid dynamics model to predict thermo-fluid and scalar transport over an incubation period using high performance computing systems. Various modeling approaches for grid generation, inflow conditions, fan and heater modeling, and scalar transport are evaluated to identify cost effective numerical models and allow long time simulations with reliable data. The model is …

Personalized Nutrition: Tailoring Dietary Recommendations Through Genetic Insights, Saiful Singar, Ravinder Nagpal, Bahram H. Arjmandi, Neda S. Akhavan

Kinesiology and Nutrition Sciences Faculty Research

Personalized nutrition (PN) represents a transformative approach in dietary science, where individual genetic profiles guide tailored dietary recommendations, thereby optimizing health outcomes and managing chronic diseases more effectively. This review synthesizes key aspects of PN, emphasizing the genetic basis of dietary responses, contemporary research, and practical applications. We explore how individual genetic differences influence dietary metabolisms, thus underscoring the importance of nutrigenomics in developing personalized dietary guidelines. Current research in PN highlights significant gene–diet interactions that affect various conditions, including obesity and diabetes, suggesting that dietary interventions could be more precise and beneficial if they are customized to genetic profiles. …

Building A Simplistic Automatic Extruder: Instrument Development Opportunities For The Laboratory, Stefanie Klisch, Dylan Gilbert, Emma Breaux, Aliyah Dalier, Sudipta Gupta, Bruno Jakobi, Gerald J. Schneider

Faculty Publications

This work presents an automatic extruder as a research experience for undergraduate students. The system offers a user-friendly approach to preparing vesicles, such as liposomes or polymersomes, with a defined size and polydispersity─properties crucial for research in biology and macromolecules. It comprises two syringe pumps connected by a membrane filter. The setup is controlled by software. Compared to manual extrusion, this automated system provides advantages, such as precisely controlled variables. The project describes a tool to enhance undergraduate learning in science and engineering laboratories. Building an automatic extruder serves as a simplified model of a complex industrial process. It offers …

Longitudinal Influences Of Parental Relationships On Positive Adolescent Functioning, Rebekah Buckley Sparks

Theses and Dissertations

This dissertation examines the longitudinal influences of parental relationships – romantic, co-parenting, and parent-child – on positive adolescent functioning. Utilizing data from the Future of Families and Child Wellbeing Study, this study investigates how various dimensions of parental relationships impact adolescent well-being at age 15, as measured by the EPOCH scale. Grounded in Attachment Theory and the Life Course Perspective, this research highlights the interconnectedness of early parental interactions and their long-term effects on adolescents’ emotional and social development. Key findings indicate that supportive and stable parental relationships significantly enhance adolescents’ engagement, perseverance, optimism, connectedness, and happiness. The study emphasizes …

The Conservation Of Brain Folding Mechanics, Amber Cook

Theses and Dissertations

The pattern and complexity of cerebellar folding varies across vertebrates. However, little is known about how the tissue-level mechanics are adjusted to create species-specific folding patterns. Here, we utilize a variety of species with differences in cerebellar folding amount to investigate two tissue-level mechanics known to affect folding—differential expansion and outer layer thickness. We found that the level of differential expansion between the outer and inner layers of the cerebellum broadly correlates with folding amount and that the thickness of the outer layer is important as thicker outer layers require more differential expansion to fold. Additionally, we find that both …

Simulating The Long-Term Response Of Forest Succession To Climate Change In The Boreal Forest Of Northern Ontario, Canada, Guy R. Larocque, F. Wayne Bell, Eric B. Searle, Stephen J. Mayor, Thomas Schiks, Parvin Kalantari

Aspen Bibliography

The effect of climate change on forest dynamics is likely to increase in importance in the forthcoming decades. For this reason, it is essential to predict the extent to which changes in temperature, precipitation, and atmospheric CO₂ might affect the development of forest ecosystems and successional pathways. The gap model ZELIG-CFS was used to simulate the potential long-term effects of climate change on species-specific annual change in mean basal area and stand density under two scenarios of representative concentration pathways (RCP), 4.5 and 8.5, for the boreal forest region of Ontario, Canada, where mean temperature, precipitation, and atmospheric CO …

Investigating The Cellular Mechanisms Of Intracellular Ph Regulation During Exposure To High Co2., Dillon Flowers

Biology Theses

There are at least two different strategies of pH regulation among fish. The first is coupled pH regulation (CPR), where regulation of blood pH facilitates tissue pH regulation; and the second is preferential intracellular pH (pHi) regulation (PPR), where tissue pH remains tightly regulated despite a sustained reduction in blood pH. The cellular and molecular mechanisms underlying the differences in pHi regulation are currently unknown. I investigated the mechanisms of pH regulation in fishes using Rainbow Trout (Oncorhynchus mykiss) and Channel Catfish (Ictalurus punctatus) which use CPR and PPR, respectively. Fish were exposed to elevated CO …

The Worry Of Food Insecurity And The Manifestation Of Generalized Anxiety In Relation To Perceived Health Status, Ramona Lynnette Taylor

Walden Dissertations and Doctoral Studies

This study examined the association between the worry of food insecurity and the manifestation of generalized anxiety, and how these factors related to perceived health status among adults aged 18 years and older in the U.S. The study controlled demographic factors such as gender, age, race, and education. Utilizing data from the 2019 National Health Interview Survey (NHIS), a binomial logistic regression analysis was employed. The findings indicated a significant association between those persons who often worry about food insecurity, severe (OR = 7.324, p < .001) and frequent generalized anxiety (OR = 5.185, p < .001) suggesting that individuals who worry about food insecurity have higher odds of experiencing severe and frequent (more than 3 times per week) generalized anxiety. Furthermore, both the worry of food insecurity in persons not sure (OR = 0.120, p = 0.004) and generalized anxiety (OR = 0.623 for severe anxiety, p = 0.009) were found to be significantly associated with lower perceived health status. These results suggest a potential association between worry about food insecurity and negative impacts on mental health and perceived overall well-being. Overall, these findings underscore the significant impact of food insecurity on mental health. Further research, including experimental and longitudinal studies, is necessary to elucidate the causal relationship between food insecurity and the manifestation of generalized anxiety in order to impact positive social change.

Zno Based Chemiresistive Sensor For Ammonia Detection, Anna Thomas

Theses and Dissertations

This work aims on the development of nanostructured sensing elements based on ZnO for detecting ammonia, specifically designed for applications in poultry and farm fields. To achieve this objective, we prepared sensing elements, including ZnO, CuO, WO3, NiO, ZnO/CuO, ZnO/WO3, ZnO/NiO, and rGO/ZnO, using the spray pyrolysis technique. Structural, morphological, and compositional analyses were studied using X-ray Diffraction (XRD), Field Emission Microscope (FESEM), and X-ray Photoelectron Spectroscopy (XPS), respectively. The vapour/gas detection studies of fabricated sensing elements were carried out in a home built sensing chamber.

The single oxides, ZnO, CuO, WO3, demonstrated ammonia detection in the range of 5-100 …

One More Negative Regulator Of Ac6: S-Nitrosylation, Isabella Cattani-Cavalieri, Rennolds S. Ostrom

Pharmacy Faculty Articles and Research

"In this issue of the Journal, Maghsoudi and colleagues (pp. 82–96) report a series of studies that delineate the biochemical and pharmacological mechanism of this resistance (4). The study focuses on adenylyl cyclase (AC), an enzyme crucial for pulmonary vasodilation through its action of synthesizing cAMP. There are nine isoforms of AC (AC1–AC9) that are all stimulated by Gs but otherwise are distinct in their regulatory qualities, subcellular localization, and expression profiles throughout the body (5). AC6 plays a significant role in fetal and neonatal lung, especially in pulmonary artery smooth muscle cells, where …

The Lived Experiences Of Clinical Staff Nurses Who Provide Care For Transgender Patients In Florida, Twanna L. Crockam

Dissertations

Historically, transgender patients have experienced challenging interactions with healthcare providers. As a result, many transgender patients refuse to seek medical attention for a broad spectrum of healthcare needs. Although the transgender population has gained attention, a lack of research exists of clinical staff nurses' lived experiences providing care for transgender patients.

The purpose of this qualitative hermeneutic phenomenological inquiry was to uncover and understand the experiences of clinical staff nurses when providing care for transgender patients in Florida. The research question was this: "What is the lived experience of clinical staff nurses who provide care for transgender patients in Florida?"

Buffering The Strained Relation Between Masculinity And Social Support With Self-Compassion, Nathan Barclay

Theses and Dissertations

Men experience high rates of negative health outcomes and often have difficulty identifying and seeking support for their issues. Traditional masculine socialization has been identified as a potential determinant of this effect, as masculine ideals often conflict with prosocial help-seeking behaviors. Men who experience this sort of socialization need better support for their mental health and are less likely to seek it out in a professional setting. Therefore, other models of support, like social support, should be investigated. Additionally, factors that impact the way that men perceive social support should be explored, one of which may be self-compassion. The current …

Evaluating The Use Of Soil Amendments For Shortleaf Pine (Pinus Echinata) Restoration On Post-Mined Landscapes, Casey Iwamoto

Theses and Dissertations

Coal strip mining leaves widespread degraded soil throughout the southeastern US. These soils tend to have low pH, high bulk density, impacted hydrologic processes, and an accumulation of heavy metals that limit revegetation and reforestation efforts. Shortleaf pine (Pinus echinata) can tolerate these poor conditions on post-mined sites and has the largest native pine range in the southeastern US, making it an ideal species for restoration efforts. Additionally, the use of soil amendments to improve soil physical and chemical parameters is expected to lead to improved plant establishment and growth. To address the challenges associated with degraded post-mined landscapes, two …

Movement Ecology And Nest-Site Selection Of Rock Iguanas Across A Gradient Of Anthropogenic Disturbance, James Walter Wehsener

Theses and Dissertations

Analysis of animal movement has been integrated into a framework of four components: internal state, motion capacity, navigation capacity, and external factors (Nathan et al., 2008). We used this framework to enhance understanding of movement behavior in the 15 West Indian rock iguana (Cyclura) taxa and to inform conservation management. Our review found that adult female Cyclura large-scale movement is primarily driven by nesting and migrations. Nest site selection also influences hatchling dispersal, but few studies identify preferred habitats for nesting females. Anthropogenic impacts on nesting ecology are largely unexplored, especially in Cyclura carinata. We studied C. carinata nesting ecology …

Early Childhood Teachers’ Perspectives On The Challenges Of Teaching Students Who Lack Age-Appropriate Social And Emotional Skills, Rachel Ann Stewart

Walden Dissertations and Doctoral Studies

The problem addressed in this study was the significant increase in the number of students who lack age-appropriate social and emotional skills (SES), which has become a concern, especially for teachers. The purpose of this basic qualitative study was to explore early childhood teachers’ perspectives on the challenges of teaching students who lack age-appropriate SES and what support they believed would help them when working with these students in the classroom. The conceptual framework that guided this study consisted of Bandura’s social learning theory. The focus of the research questions was early childhood teachers’ perspectives on the challenges of teaching …

Efficacy Of Recycled Glass Cullet As A Soil Substrate For U.S. Gulf Coast Salt Marsh Restoration, Ansley Levine

Theses and Dissertations

Recycled glass cullet is a potential alternative to fill substrate in salt marsh restoration projects, given its similarity to natural silica sands. However, the ability for common salt marsh plants to grow in glass cullet has not yet been tested. An outdoor mesocosm experiment assessed the ability of the dominant mid-marsh plant Juncus roemerianus and the upland marsh plant Spartina patens to grow in various mixtures of glass cullet versus fill soil. Results showed that J. roemerianus can survive in glass cullet, but growth declined as the proportion of cullet increased. However, S. patens grew comparably, if not better, in …

Insulin-Independent Regulation Of Type 1 Diabetes Via Large Brown Adipocyte-Secreted Proteins And The Novel Glucagon Regulator, Nidogen2, Jeongmin Lee

Arts & Sciences Graduate Student Theses and Dissertations

Current treatments for type 1 diabetes (T1D) focus on insulin replacement. We demonstrate the therapeutic potential of a secreted protein fraction from embryonic brown adipose tissue (BAT), independent of insulin. The large molecular weight secreted fraction mediates insulin receptor-dependent recovery of euglycemia in a T1D animal model, nonobese diabetic (NOD) mice, by suppressing glucagon secretion. This fraction also promotes white adipocyte differentiation and browning, maintains healthy BAT, and enhances glucose uptake in adipose tissue, skeletal muscle, and liver. From this fraction, we identify nidogen-2 as a critical BAT-secreted protein that reverses hyperglycemia in NOD mice, inhibits glucagon secretion from pancreatic …

Re: Butte Priority Soils Operable Unit (Bpsou) Revised Draft Final Insufficiently Reclaimed Sites Field Sampling Plans (Fsps) And Responses To Agency Comments, Mike Mcanulty

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

A Comparative Analysis Of The Immunoglobulin Repertoire In Leukemia Cells And B Cells In Chinese Acute Myeloid Leukemia By High-Throughput Sequencing, Huige Yan, Lina Wu, Pingzhang Wang, Miaoran Xia, Zhan Shi, Xinmei Huang, Sha Yin, Qian Jiang, C Cameron Yin, Xiangyu Zhao, Xiaoyan Qiu

Faculty, Staff and Student Publications

It is common knowledge that immunoglobulin (Ig) is produced by B lymphocytes and mainly functions as an antibody. However, it has been shown recently that myeloblasts from acute myeloid leukemia (AML) could also express Ig and that AML-Ig played a role in leukemogenesis and AML progression. The difference between Ig from myeloblasts and B cells has not been explored. Studying the characteristics of the Ig repertoire in myeloblasts and B cells will be helpful to understand the function and significance of AML-Ig. We performed 5' RACE-related PCR coupled with PacBio sequencing to analyze the Ig repertoire in myeloblasts and B …

Mathematical Modeling, Analysis, And Simulation Of Patient Addiction Journey, Adan Baca, Diego Gonzalez, Alonso G. Ogueda, Holly C. Matto, Padmanabhan Seshaiyer

CODEE Journal

This paper aims to develop a mathematical model to study the dynamics of addiction as individuals go through their detox journey. The motivation for this work is three fold. First, there has been a significant increase in drug overdose and drug addiction following the COVID-19 pandemic, and addiction may be interpreted as a infectious disease. Secondly, the dynamics of infectious disease could be modeled via compartmental models described by differential equations and one can therefore leverage the existing analytical and numerical methods to model addiction as a disease. Finally, the work helps to inform how mathematical models governed by differential …