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Small Molecule Inhibitors Targeting Clostridioides Difficile Sporulation, Yu Chen, Michael Sacco, James Leahy, Elena Bray, Xingmin Sun, Xiujun Zhang

USF Patents

Disclosed herein are compounds that can prevent and treat C. difficile by inhibiting sporulation. Also disclosed herein are compositions and formulations comprising the compound disclosed herein. Methods of preventing and treating C. difficile comprising administering the compounds described herein are also disclosed.

Neuroepithelial Bodies And Terminal Bronchioles Are Niches For Distinctive Club Cells That Repair The Airways Following Acute Notch Inhibition, Sai Manoz Lingamallu, Aditya Deshpande, Neenu Joy, Kirthana Ganeshan

Open Access archive

Lower airway club cells (CCs) serve the dual roles of a secretory cell and a stem cell. Here, we probe how the CC fate is regulated. We find that, in response to acute perturbation of Notch signaling, CCs adopt distinct fates. Although the vast majority transdifferentiate into multiciliated cells, a “variant” subpopulation (v-CCs), juxtaposed to neuroepithelial bodies (NEBs; 5%–10%) and located at bronchioalveolar duct junctions (>80%), does not. Instead, v-CCs transition into lineage-ambiguous states but can revert to a CC fate upon restoration of Notch signaling and repopulate the airways with CCs and multiciliated cells. The v-CC response to …

Cd103+ Cdc1 Dendritic Cell Vaccine Therapy For Osteosarcoma Lung Metastases, Yuanzheng Yang, Yifan Zhou, Jian Wang, You Zhou, Stephanie S Watowich, Eugenie S Kleinerman

Faculty, Staff and Student Publications

Background: We generated a CD103+DC vaccine using K7M3 OS cell lysates (cDCV) and investigated its ability to induce regression of primary tumors, established lung metastases, and a systemic immune response. Methods: A bilateral tumor model was used to assess cDCV therapy efficacy and systemic immunity induction. K7M3 cells were injected into mice bilaterally. Right-sided tumors received PBS (control) or cDCV. Left-sided tumors were untreated. Tumor growth was compared between the vaccine-treated and untreated tumor on the contralateral side and compared to the control group. The immune cell profiles of the tumors, and tumor-draining lymph nodes (TdLNs) and spleen were evaluated. …

Mental Health During The Covid-19 Pandemic In Trinidad: An Analysis Of Depression, Anxiety, And Stress Among Essential Workers, Bernadette Marson, Gerard A. Hutchinson

Journal of Social, Behavioral, and Health Sciences

Research shows that some essential workers, such as healthcare workers, social workers, and first responders (including police officers, paramedics, and firefighters), are at greater risk for developing both short- and long-term mental health problems (Berger et al., 2012; Canady, 2022; Grey et al., 2020; Haugen et al., 2012; Mohamed et al., 2022; Petrie et al., 2018). The most prevalent of these include anxiety, depression, and stress, as well as other social and cognitive problems—with stress causing harmful effects on mental health and potentially resulting in anxiety and depression, which may then disrupt an individual’s social relationships, family life, and work. …

Projected Near-Future Flooding And Warming Increase Graminoid Biomass In A High-Latitude Coastal Wetland, Matteo Petit Bon, A. Joshua Leffler, Katharine C. Kelsey, Tyler J. Williams, Karen H. Beard

Wildland Resources Faculty Publications

1. With rapid climate warming, some coastal high-latitude ecosystems are experiencing more frequent tidal floods. Yet little is known about tundra plant-community responses to flooding, and whether Arctic warming may modulate such responses.

2. In a 2-year, full-factorial field experiment in coastal tundra wetlands of the Yukon-Kuskokwim (Y-K) Delta (western Alaska), we simulated periodic tidal flood events at two severities under both ambient and warmed summer conditions and measured above-ground plant-community responses. Low-severity flooding represented overbank flooding 1 day per month, which is consistent with projections in the next 5 years. High-severity flooding represented a more impactful flooding regime (three consecutive days …

Immunity And Protective Efficacy Of A Plant-Based Tobacco Mosaic Virus-Like Nanoparticle Vaccine Against Influenza A Virus In Mice, Adthakorn Madapong, Erika M. Petro-Turnquist, Richard J. Webby, Allison A. Mccormick, Eric A. Weaver

School of Biological Sciences: Faculty Publications

Background: The rapid production of influenza vaccines is crucial to meet increasing pandemic response demands. Here, we developed plant-made vaccines comprising centralized consensus influenza hemagglutinin (HA-con) proteins (H1 and H3 subtypes) conjugated to a modified plant virus, tobacco mosaic virus (TMV) nanoparticle (TMV-HA-con). Methods: We compared immune responses and protective efficacy against historical H1 or H3 influenza A virus infections among TMV-HA-con, HA-con protein combined with AddaVax™ adjuvant, and whole-inactivated virus vaccine (Fluzone®). Results: Immunogenicity studies demonstrated robust IgG, IgM, and IgA responses in the TMV-HA-con and HA-con protein vaccinated groups, with relatively low induction of interferon (IFN)-γ⁺ T-cell …

Characterizing Biomolecular Structure Features Through An Innovative Elliptical Dichroism Spectrometry For Cancer Detection, Yusuf Asad, Keerthi Priya Jangili, Amara Arshad, Maliha Elma, Komila Rasuleva, Alfred Akinlalu, Tommy Gao, Umamaheswara Rao Tida, Wenjie Xia, Dali Sun

Electrical and Computer Engineering: Faculty Scholarship

This research introduces a novel method for evaluating the structural features of biomolecules, utilizing our innovative Elliptical Dichroism (ED) spectrometer specifically designed for stereochemical analysis. By integrating ED spectrometry with autocorrelation (AC) analysis, we investigate the conformational characteristics of biological molecules such as amino acids, proteins, and extracellular vesicles (EVs) induced by elliptically polarized UV absorption. Our streamlined approach offers a cost-effective and portable solution with minimal sample consumption and supports multiple working modes to efficiently characterize biomolecular structures. The insight from this new approach demonstrates potential applications in using biomolecular characterization for cancer detection.

In Vivo Crispr Screens Identify Mga As An Immunotherapy Target In Triple-Negative Breast Cancer, Xu Feng, Chang Yang, Yuanjian Huang, Dan Su, Chao Wang, Lori Lyn Wilson, Ling Yin, Mengfan Tang, Siting Li, Zhen Chen, Dandan Zhu, Shimin Wang, Shengzhe Zhang, Jie Zhang, Huimin Zhang, Litong Nie, Min Huang, Jae-Il Park, Traver Hart, Dadi Jiang, Kuirong Jiang, Junjie Chen

Faculty, Staff and Student Publications

Understanding the mechanisms underlying immune evasion is crucial for developing novel anticancer modalities. To systematically uncover tumor-intrinsic genetic modulators involved in immune escape in tumor microenvironment, we performed genome-scale in vivo CRISPR screens in two syngeneic models and later expanded up to seven syngeneic models with a focused validation library. These data help us better understand tumor immune evasion and pave the way for developing effective therapeutics. Importantly, we uncovered that Mga depletion elicited an antitumor immune response and inhibited tumor growth in triple-negative breast cancer. Our findings suggest that Mga may play a role in modulating the tumor immune …

Rapidly Evaluating Species At Risk Using Endemic Plants Of Kosrae, Federated States Of Micronesia, Wayne Law, Megan Chellew, Kenneth R. Wood, David H. Lorence, Carlos Cianchini, Jacob Sanney

Faculty and Staff Publications & Presentations

The International Union for Conservation of Nature (IUCN)'s Red List of Threatened Species is regarded as the primary source of global extinction risk for species. Despite this importance, the IUCN has assessed less than 20% of the world′s estimated 400,000 flowering plants due to issues like insufficient data or a lack of experts. Thousands of conservation status assessments were generated recently in response to the call to action by the revised Target 2 of the Global Strategy for Plant Conservation (GSPC), “An assessment of the conservation status of all known plant species, as far as possible, to guide conservation action.” …

Stimulation Of An Entorhinal-Hippocampal Extinction Circuit Facilitates Fear Extinction In A Post-Traumatic Stress Disorder Model, Ze-Jie Lin, Xue Gu, Wan-Kun Gong, Mo Wang, Yan-Jiao Wu, Qi Wang, Xin-Rong Wu, Xin-Yu Zhao, Michael X Zhu, Lu-Yang Wang, Quanying Liu, Ti-Fei Yuan, Wei-Guang Li, Tian-Le Xu

Faculty, Staff and Student Publications

Effective psychotherapy of post-traumatic stress disorder (PTSD) remains challenging owing to the fragile nature of fear extinction, for which the ventral hippocampal CA1 (vCA1) region is considered as a central hub. However, neither the core pathway nor the cellular mechanisms involved in implementing extinction are known. Here, we unveil a direct pathway, where layer 2a fan cells in the lateral entorhinal cortex (LEC) target parvalbumin-expressing interneurons (PV-INs) in the vCA1 region to propel low-gamma-band synchronization of the LEC-vCA1 activity during extinction learning. Bidirectional manipulations of either hippocampal PV-INs or LEC fan cells sufficed for fear extinction. Gamma entrainment of vCA1 …

Predictors Of Transition From Child And Adolescent Bipolar Not Otherwise Specified To Bipolar I Disorder, A Longitudinal 3.9-Year Study., María Ribeiro-Fernández, Azucena Díez-Suárez, Kiki D Chang, Cesar A Soutullo

Faculty, Staff and Student Publications

Background: Children and adolescents with subthreshold manic symptoms not meeting full DSM criteria for bipolar I or II disorder (BP-I or BP-II) are classified as unspecified bipolar disorder (formerly bipolar not otherwise specified: BP-NOS). Factors associated with transition from BP-II or NOS to BP-I may predict the progression of the disorder. Our objective is to analyze factors associated with transition to BP-I in a Spanish sample of youth with BP-NOS or BP-II.

Methods: We included all youth diagnosed with BP before 18 years of age presenting to our clinic (October 1999-December 2014). We assessed clinical factors that may predict transition …

Conformational Ensembles In Klebsiella Pneumoniae Fimh Impact Uropathogenesis, Edward D B Lopatto, Jerome S Pinkner, Denise A Sanick, Robert F Potter, Lily X Liu, Jesús Bazán Villicaña, Kevin O Tamadonfar, Yijun Ye, Maxwell I Zimmerman, Nathaniel C Gualberto, Karen W Dodson, James W Janetka, David A Hunstad, Scott J Hultgren

2020-Current year OA Pubs

No abstract provided.

Cd8Α Structural Domains Enhance Gucy2c Car-T Cell Efficacy, Trevor R. Baybutt, Ariana A. Entezari, Adi Caspi, Ross E. Staudt, Robert D. Carlson, Scott A. Waldman, Adam E. Snook

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

Despite success in treating some hematological malignancies, CAR-T cells have not yet produced similar outcomes in solid tumors due, in part, to the tumor microenvironment, poor persistence, and a paucity of suitable target antigens. Importantly, the impact of the CAR components on these challenges remains focused on the intracellular signaling and antigen-binding domains. In contrast, the flexible hinge and transmembrane domains have been commoditized and are the least studied components of the CAR. Here, we compared the hinge and transmembrane domains derived from either the CD8ɑ or CD28 molecule in identical GUCY2C-targeted third-generation designs for colorectal cancer. While these structural …

Olfactory Deficit And Gastrointestinal Dysfunction Precede Motor Abnormalities In Alpha-Synuclein G51d Knock-In Mice, Youngdoo Kim, Joseph Mcinnes, Jiyoen Kim, Yan Hong Wei Liang, Surabi Veeraragavan, Alexandra Rae Garza, Benjamin David Webst Belfort, Benjamin Arenkiel, Rodney Samaco, Huda Yahya Zoghbi

Faculty, Staff and Students Publications

Many Parkinson’s disease (PD) models overexpress α-Synuclein using heterologous promoters, which is adequate to demonstrate that excessive α-Synuclein is toxic but not ideal for learning the precise ontogeny of the disease pathogenesis, specifically where the disease starts and how it progresses. To answer these questions, it is beneficial to generate a mouse model expressing a disease-causing mutation under the endogenous promoter. Here, we generated three Snca knock-in mice. Among them, homozygous Snca^G51D mice develop motor deficits by 9 mo of age. These mice exhibit olfactory and gastrointestinal abnormalities by 6 mo. They lose dopaminergic neurons and have reduced dopamine …

From Beginning To Beginning: Fostering Vulnerability As A Force For Dismantling Teaching & Learning Hierarchies, Leigh Ferrier, Daniel Pieczkolon, Ellen Skilton

Teaching and Learning Together in Higher Education

No abstract provided.

Inclusivity In The Classroom: Shared Experiences Of A Student-Faculty Partnership, Kelly Basile, Cynthia Brito

Teaching and Learning Together in Higher Education

No abstract provided.

Diversity And Inclusion Are Not The Goal Anymore: Developing Inclusive Practices Is, Ebony Graham

Teaching and Learning Together in Higher Education

No abstract provided.

Insisting On Inclusive Practices, Alison Cook-Sather

Teaching and Learning Together in Higher Education

No abstract provided.

Development Of Intensive Grassland Systems: From Science To Practice, D G. Mccall, G W. Sheath

IGC Proceedings (1977-2023)

Level of nnimal production from grassland is determined by the amount of pasture grown, pasture utilisation and animal conversion efficiency. The key components affecting production are pasture growth and animal conversion efficiency. These arc mainly influenced by environment, genetics and through inputs to maintain soil fertility or animal health. Systems development aims to link pasture growth and animal conversion through efficient strategies for pasture utilisation. The means of influencing pasture utilisation is through adjustments to animal feed demand, feed supply or grazing management. Stock policies that adjust the pattern and level of feed demand are of greatest significance in developing …

The Ecology Of Grazing Management, R K. Heitschmidt

IGC Proceedings (1977-2023)

The broad objective of this paper is to review the fundamental principles of grazing management as a means of assessing the inherent value of intensive and extensive grazing systems. Conceptual analyses of intensive and extensive grazing systems reveal that the fundamental differences between intensive and extensive grazing systems are related to differences in non-grazing rather than grazing tactics. Intensive grazing systems employ a wide array of non-grazing tactics in an attempt to overcome the inherent ecological constraints that limit secondary (i.e., livestock) productivity in extensively grazed systems. Moreover, analyses of the ecological or true efficiency of various agricultural production systems …

An Analysis Of The Dry Matter Intake And Rate Of Live-Weight Gain Of Growing Cattle Fed Forage Diets, J G. Buchanan-Smith

IGC Proceedings (1977-2023)

The intake and rate of live-weight gain of 500 growing steer calves fed experimental forage diets in 7 trials were compared with corresponding values predicted using the Na1ional Research Council (NRC) model (1984). The cattle were predominantly weaned calves and most of the experimental diets were alfalfa-grass silage, either unsupplemented or supplemented with various protein sources, but some diets were based on maize silage or maize grain. The feeding period evaluated in this study ranged between the first 84 and 147 days when protein accretion rate in the cattle would be relatively high. Dry matter intakes were overpredicted by the …

Management Of Extensive Pastures For Dairy Youngstock, G.E J. Fisher, D J. Roberts

IGC Proceedings (1977-2023)

The effects of low input systems on the ground cover of species and yield and chemical composition of herbage cul from extensive lowland swards was studied over 3 years. The live-weight gain of dairy heifer replacements offered grazed or conserved forage from these pastures was also assessed, The swards were sown with a mixture of grasses, wildflowers and herbs and received no inorganic fertilizers, Applications of slurry in January and February increased herbage yield by 43%, but reduced the cover of flowers and herbs. Using a 2- cut conservation system improved yield of metabolisable energy/ha in herbage by 33%, and …

Intensive Grazing Of Alfalfa By Beef Steers, M Rasnake, W R. Burris, G D. Lacefield, J Randolph, N Gay, L Davis

IGC Proceedings (1977-2023)

In the temperate region of North America, high temperature and moisture deficit cause low forage growth during the summer of most years, making it difficult to grow beef (Bos taurus), calves through summer grazing. Alfalfa (Medicago sativa), offers some potential to provide high dry matter production during summer if an effective system of grazing can be developed. The objective of this study was to test a system of intensive grazing of beef steers on alfalfa to maximise live-weight gains/ha and maintain plant population. Alfalfa was no-till seeded into a 6.5 ha field in autumn 1988 after …

Variance Across Years In Growth Of Cattle On Annual Pastures In A Mediterranean Climate, K D. Greathead, J Zorrilla Rios

IGC Proceedings (1977-2023)

Annual pastures were continuously grazed by new drafts of yearling cattle each year for 4 years at 2 sites. The variability of weight gained across years at any date was not different between sites. The level of rainfall in April, but strongly influenced weight gained by the cattle in any year. Variances in weight change were small up to April, they increased rapidly from April to late July and were not different after July (P<0.05).

Modification Of The Botanical Composition Of Perennial And Annual Temperate Pastures By Strategic Grazing Practices, D R. Kemp, P M. Dowling

IGC Proceedings (1977-2023)

Where livestock production depends upon pastures, management is more difficult in environments where rainfall varies considerably from season to season and year to year. Flexible grazing practices are needed to optimise species composition and pasture productivity. Results presented in this paper show that pasture composition can be improved within climatic constraints, by grazing practices within seasons, In temperate annual pastures, long rests in winter (similar to a long rotation during that season) increased annual legume content in early spring from 20 to 50%. Annual grass content was unchanged. In perennial (phalaris) pastures, the dominant legume can change over years from …

Development Of Grass-Clover Pasture Systems For Autumn-Calving Beef Cows. I. Forage Production, G D. Mooso, D G. Morrison, J I. Feazel, D R. Morris

IGC Proceedings (1977-2023)

Four 8-ha areas of bermudagrass-based (Cynodon dactylon (L.) Pers.) grazing systems were sodseeded and received annual N fertiliser as follows: I) clover (Trifolium sp.)+81 kg Nnm, 2) annual ryegrass (Lolium multiflorum Lam)+clover +81 kg N/ha, 3) ryegrass+clover+l39 kg N/ ha, and 4) ryegrass+139 kg N/ha. Monthly forage accumulation was estimated for 3 years by clipping samples from 2 exclosures/pasture. Environmental conditions had a significant effect on total annual forage dry matter (DM) production. Systems 3 and 4 produced more (P<0.05) forage DM than Systems 1 and 2 (11.0 v. 10,3 Mg/ha). System I produced more (P<0.l) forage DM than 2 (10.6 ll, 9.8 Mg/ha). Systems 3 and 4 were similar, producing I 1.0 Mg/ha. Monthly forage accumulation was lowest in winter (December to February) and highest in spring and summer (March to August). The results of this study demonstrate that at a low N level, there was no benefii from including ryegrass in the system and al a moderate N level, there was no benefit from including clover.

Development Of Grass-Clover Grazing Systems For Autumn-Calving Beef Cows. Ii. Cow-Calf Production And Economic Analysis, D G. Morrison, J I. Feazel, G D. Mooso

IGC Proceedings (1977-2023)

Four 8-ha areas of bermudagrass-based (Cynodon dactylon (L.) Pers.) grazing systems were sod seeded and received annual N fertiliser as follows: I) clover (Trifolium sp.)+81 kg/ha, 2) annual rye grass (Lolium multiflorum Lsm.)+clover +81 kg/ha, 3) ryegrass+clover+139 kg/ha, and 4) ryegrass+l39 kg/ha. Each system was stocked year-round with 20 (2,5/lla) cows each with crossbred suckling calf. Cow and calf performance data for 3 of 4 years are included. Year affected (P<0.01) cow and calf live weight, Cows in System 2 were 25-50 kg lower in live weight in autumn and winter than cows in other systems. From February to July, cow daily live-weight gains were 0.11 kg higher (P<0.01) for Systems 2 and 3, Cow live weight in July was affected by N level (Systems 1,2<3,4; P<0,01), Year by system interactions (P<0.05) occurred for cow live weight in February, April and July because of live weight increasing over years in Systems 3 and 4 but not in Systems 1 and 2. Calf daily live-weight gain February to weaning (July) was higher (P<0.01) for System 2 v. 1 (0.06 kg), Syuem 3 v. 4 (0.07 kg), and Systems 3, 4 v. 1, 2 (0.07 kg), Estimated net returns favoured Systems 3 and 4 over Systems 1 and 2.

Improved Grazing Systems For Lamb Production On Appalachian Hill Farms, R L. Reid, J R. Puoli, G A. Jung, D P. Belesky

IGC Proceedings (1977-2023)

Improved grazing methods were tested to increase lamb outputs per ha from Appalachian hill farms. Components of a grazing system were cool-season grass [tall fescue (Festuca arundinacea Schreb.), Grasslands Matua prairie grass (Brom us willdenowii Kunth)J or grass­legume [Kentucky bluegrass (Poa pratensis L.)•red clover (Trifolium pralense L.)] pastures in spring and early autumn, warm-season grass [switchgrass (Panicum virgatum L.)] in summer, and brassicas in late autumn and early winter. Matua was highly productive in cool weather but was subject to fungal infection and grazing ewes had low Mg status in spring. Rotationally grazed switchgrass maintained high numbers …

Use Of Brassica Forage Crops To Extend The Grazing Season In The North-East Usa, G A. Jung, H W. Harpster, M G. Lambert, K T. Leath, R A. Byers

IGC Proceedings (1977-2023)

Forage turnip (Brassica rapa L,) can be made available for animal utilisation in late summer or In late autumn-early winter, when cool­season grass pastures have a low animal carrying capacity. field trials were conducted to identify and quantify factors that influence turnip yield in December or January and to determine whether these factors affect turnip cultivars similarly. Different levels of production in late autumn-winter were attributed to summer drought, foliar diseases, freezing temperatures and rate of leaf senescence. In all instances, the effects of these factors varied with turnip cultivar. However, cultivar ranking generally was the same whether mean …

Interactions Of Rotational And Continuous Grazing With Stocking Rate On Warm And Cool-Season Pastures, David I. Bransby

IGC Proceedings (1977-2023)

With more intense commercialisation of electric fencing many production and economic advantages have been claimed for rotational grazing compared with continuous grazing. However, very little research data are available from the south-eastern USA to verify these claims, Therefore, the objective of this study was to evaluate animal performance under conditions of continuous and IO-paddock rotational grazing on cool- and warm-season pastures commonly used in this region. Three 2-year grazing experiments were conducted, one each on a mixed rye-annual ryegrass (Secale cereale-Lolium multiflorum) pasture, Coastal bermudagrass ( Cynodon hybrid) and Pensacola bahiagrass (Paspalum notatum). In each experiment continuous …