Digital Commons Network^™

3d Bioprinted Coaxial Tubule Optimization For Human Aortic Endothelial Cell Growth, Ilse Gabriela Perez Garcia

Theses and Dissertations

This paper introduces a research study of printing parameter optimization of extruded, cell-laden, 3D bioprinted coaxial tubules, as well as cell interaction observations. Optimization of printing parameters seeks to reduce variability of the tubules’ inner and outer diameters. The tubules were manufactured from a 0.4% Collagen I - 1.6% NaC6H7O6 (Sodium Alginate) bioink, that reaches gelation through interaction with a 3.2% CaCl2 (Calcium Chloride) solution. The bioinks were extruded through a dual syringe pump mechanism connected to a coaxial nozzle, which is mounted on an adapted 3D FDM (Fused Deposition …

Student Loan Debt Disparity Among White, African American And Hispanic Women: A National View, Arnoldo Trejo

Theses and Dissertations

Published research into the student loan debt problem reveals a disproportionate impact on underrepresented groups within higher education, notably Hispanics, African Americans, and women students. Research on student loan debt underscores that lower-income and first-generation college students are significantly more affected when compared to their higher-income counterparts. The escalating trends in student loan debt are forcing college-going students in the United States to postpone major life events such as homeownership, marriage, and starting a family. The burden of high student debt negatively influences the accumulation of family wealth, including critical aspects such as home ownership, retirement savings, investments, and other …

Understanding Social Media As A Communicative Tool To Facilitate Critical Discourse: Using #Blackgirlmagic To Explore How Black Women In Higher Education “Talk Back", Crystal Spruill Carter

Theses and Dissertations

Black women have engaged through social media platforms, as a mechanism to uplift their embodiment, despite being relegated to the margins. The hashtag #BlackGirlMagic (BGM) is linked to narratives of Black women in higher education that are empowering and problematic. The development of the popular social media movement during a time when Black women were being portrayed negatively made engaging in the discourse "talking back" possible. This study explores the lived experiences of Black women college students and recent graduates, using currere as an analytical tool for my own communication journals entries as a data source to structure my reflections …

Examining Hispanic Beginning Farmers And Ranchers In The Rio Grande Valley Through A Community Capitals Framework, Perla Kimberly Schrock

Theses and Dissertations

While existing literature addresses the experiences of beginning farmers and ranchers (BFR), there is a notable gap in research focused on Hispanic beginning farmers and ranchers (HBFR). This study aimed at exploring the unique experiences of HBFR in a predominantly Hispanic region, the Rio Grande Valley (RGV), employed the Community Capitals Framework (Flora et al., 2016). I conducted 16 semi-structured interviews with HBFR and local nonprofit organizations and federal employees, complemented by participant observations. Through thematic and content analysis of the interview transcripts, findings revealed valuable insights into the livelihoods of HBFR. Despite HBFR facing significant challenges, this study demonstrated …

Leveraging A Community Of Practice To Advance Stem Education Reform And Promote Teacher And Student Self-Efficacy, Ruth Renee Colyer

Theses and Dissertations

This dissertation examined the impact of the CREST-MECIS community of practice on K-12 STEM teachers and undergraduate and graduate students, addressing the urgent need for innovative approaches in science education to meet the challenges of the 21st century. The study was grounded in response to three primary problem statements: 1) the significant learning losses and socio-emotional challenges stemming from the COVID-19 pandemic, 2) the inadequacy of traditional instructional methods in post-pandemic classrooms, and 3) the need for culturally relevant and inclusive teaching practices that acknowledge students’ lived experiences. Data were collected through pre- and post-interviews, survey analyses, and qualitative feedback …

Assessing Cover Crop Adoption Factors In The Rio Grande Valley Through A Social-Ecological Perspective, Mackenzie Feldman

Theses and Dissertations

Reducing agriculture’s negative environmental impacts is crucial for mitigating climate change (White, 2021). Cover crops– non-cash crops grown to benefit subsequent cash crops– can slow erosion, improve soil health, enhance water availability, control pests, and increase biodiversity. Despite these benefits, adoption rates remain low, prompting the USDA and other organizations to fund research on increasing their use. Most studies focus on the Midwest and Northeast, and emphasize individual factors over the relationship between structural factors and individual factors that impact farmers’ decisions to adopt cover crops. This USDA-funded study begins to fill this gap by exploring social-ecological factors and how …

Emotion Regulation From A Virtue Perspective: Applications For Individuals With Adverse Childhood Experiences, Maria Eugenia Palomo

Theses and Dissertations

The purpose of this study is to explore the contributory effect of Virtues in differentiating one’s ability to regulate emotion. Convenience sampling will be utilized to obtain a total of 102 participants who had adverse childhood experiences. Participants will be recruited via collaboration with local behavioral counseling agencies. Two behavioral health counseling agencies located in Hidalgo County, including Corazon Consulting, Inc. and A Better Tomorrow, LLC, have agreed to be part of the present study. The research packets used in this study consisted of two parts: 1) a demographic information form, and 2) a research questionnaire that includes three instruments …

Resonances Of Electrostatically Actuated Non-Uniform Mems Cantilever Resonator Of Constant Width And Linearly Varying Thickness, Rigoberto Flores

Theses and Dissertations

In this investigation the behavior of an electrostatically actuated non-uniform microelectromechanical (MEMS) cantilever resonator of constant width and linearly varying thickness is investigated. The cantilever resonator vibrates using soft AC voltage at near half the first natural frequency of the cantilever and near the first natural frequency of the cantilever, known as primary and parametric resonances, respectively. Bifurcation diagrams for the amplitude-frequency and amplitude-voltage response are obtained for both resonances. Two methods are used in this investigation, namely the Method of Multiple Scales (MMS) and Reduced Order Model (ROM) using up to 6 modes of vibration.

These methods are used …

Detecting Anomalies In Dynamic Attributed Graphs: An Unsupervised Learning Approach, Austin Hamilton

Electronic Theses and Dissertations

Dynamic attributed graphs, which evolve over time and hold node-specific attributes, are essential in fields like social network analysis, where anomalous node detection is a growing area. Vehicular social networks (VSNs), a subset of these graphs, are ad hoc networks in which vehicles exchange data with one another and with infrastructure. In this dynamic context, identifying anomalous nodes is challenging but crucial for maintaining trust within the network. This work presents an unsupervised deep learning approach for anomalous node detection in VSNs. This model achieved an accuracy of 71% while detecting synthetic anomalies in a simulated network based on real-world …

Recruitment Sources Of Grass Carp (Ctenopharyngodon Idella) In The Great Lakes, Nicholas Steven Etter

Theses

Grass Carp (Ctenopharyngodon idella) is a species of concern in areas they have invaded, such the Laurentian Great Lakes, due to their potential to substantially reduce aquatic macrophyte coverage. Efforts to control Grass Carp in the Great Lakes have primarily been focused on two tributaries to the western basin of Lake Erie (i.e., Sandusky and Maumee rivers, Ohio) where reproduction has been documented, although other Great Lakes tributaries are known to have thermal and hydrological regimes suitable for Grass Carp spawning. Knowledge of sources contributing to the expanding population of invasive Grass Carp in the Great Lakes is key to …

Propolis As An Adjuvant For Colon Cancer Chemotherapy: Exploring Its Potential On Apoptosis, Cell Cycle, And Pi3k Expression, Suryani Eka Mustika, Aznan Lelo, Iqbal Pahlevi Nasution, Poppy Anjelisa Hasibuan, Putri Chairani Eyanoer, Muhammad Ichwan, Rustam Effendi, Mustofa

Baghdad Science Journal

Propolis as co-chemotherapy is believed to reduce the severity of chemotherapy's adverse effects. The combination of propolis and chemotherapy is expected to induce apoptosis, arrest the cell cycle, and downregulate the expression of Phosphatidylinositol-3 kinase (PI3K), thereby reducing the proliferation of colon cancer cells (WiDr). The WiDr cells were obtained from the parasitology lab of the medical school at Universitas Gadjah Mada in Yogyakarta, Indonesia. Propolis is collected by beekeepers in Kabanjahe, North Sumatra. Cytotoxicity of the WiDr cell line was tested using the Microculture Tetrazolium Technique (MTT) assay. The therapy group was divided into seven subgroups. The experiment involved …

Syntheses, Characterisation, Thermal Analysis And Theoretical Studies Of Some Imino Ethanone Metal Complexes, Safaa H. Ali, Saad S. Mohammed, Hadi T. Obaid, Sanjeewa Gamagedara

Baghdad Science Journal

The current study is designed to synthesize four new bidentate metal complexes [MCl2L1] M = Co, Cu and L1 = 1,2-Diphenyl-2-(phenylimino)-1-ethanone or [MCl2L2] M = Co, Cu and L2 = 1,2-Diphenyl-2-(p-tolylimino)-1-ethanone. In addition, theoretical study preforms to predict the chemical reactivity and stability of the prepared complexes. Thus, the density function theory (DFT) studies, quantum chemical descriptors like chemical hardness (η), electronic chemical potential (μ), and electronegativity (χ) are considered. Complexes were synthesized in a simple one-pot reaction and chemical structures confirmed by different analysis techniques such as Mass spectra, FT-IR, UV-Vis spectroscopies and thermo gravimetric analysis (TGA). The prepared …

Correlation Between Particulate Matter 10 (Pm10) Exposure Time And Black Dots In Buccal Cells Of Medan Landfill Recycling Workers, T. K. Intan, M. Ichwan, D. Munir, N. N. Soeroso, A. P. Tarigan, Z. Yamamoto, N. C. Siregar

Baghdad Science Journal

This research explores the link between particulate matter (PM10) exposure and number of black dots in buccal epithelial cell nuclei in recycling workers (PDU), at the Medan City landfill, Indonesia. The study aims to identify the correlation PM10 levels, pollutant characteristics at the landfill, the appearance of AgNOR-expressed black dots in PDU groups. The measurements of PM10 exposure were carried out in open landfills in Medan city, and the subject of study is recycling workers who worked in landfills. Buccal epithelial cells were collected using an oral cytobrush smear, and the black dots were examined cytologically. Statistical analysis assessed the …

University Students With Attention-Deficit/Hyperactivity Disorder: Sex Differences In Identification And Diagnosis, Lisa Mickelson

Dissertations

University students with attention-deficit/hyperactivity disorder (ADHD) face several risk factors and challenges compared to peers without ADHD that can impede their academic success and graduation. It is important to identify and diagnose ADHD prior to entering postsecondary settings to provide students with the best academic trajectory. This is particularly true as the number of students enrolled at universities with ADHD increases. Historically, ADHD has been considered a disorder with a childhood onset. However, past research primarily focused on males. More recent studies suggest females have a different ADHD etiological presentation than males, and that onset is more likely to occur …

Physicochemical Property Effects On Immune Modulating Polymeric Nanoparticles: Potential Applications In Spinal Cord Injury, Daniel J. Kolpek, Jaechang Kim, Hisham Mohammed, John C. Gensel, Jonghyuck Park

Markey Cancer Center Faculty Publications

Nanoparticles (NPs) offer promising potential as therapeutic agents for inflammation-related diseases, owing to their capabilities in drug delivery and immune modulation. In preclinical studies focusing on spinal cord injury (SCI), polymeric NPs have demonstrated the ability to reprogram innate immune cells. This reprogramming results in redirecting immune cells away from the injury site, downregulating pro-inflammatory signaling, and promoting a regenerative environment post-injury. However, to fully understand the mechanisms driving these effects and maximize therapeutic efficacy, it is crucial to assess NP interactions with innate immune cells. This review examines how the physicochemical properties of polymeric NPs influence their modulation of …

Independent Evolution Of Oleate Hydratase Clades In Bacillales Reflects Molecular Convergence, Robert J. Neff, Priscilla C. Lages, Shannon K. Donworth, James D. Brien, Christopher D. Radka

Markey Cancer Center Faculty Publications

Oleate hydratase (OhyA), a flavoenzyme that catalyzes the hydration of unsaturated fatty acids, has been identified in various Bacillales organisms, including those in the Listeria, Lysinibacillus, Paenibacillus, and Staphylococcus genera. In this study, we combine structural biology with molecular and phylogenetic analyses to investigate the evolutionary dynamics of the OhyA protein family within the Bacillales order. Our evolutionary analysis reveals two distinct OhyA clades (clade I and clade II) within Bacillales that, while sharing catalytic function, exhibit significant genomic and structural differences. Our findings suggest that these OhyA clades originated from independent evolutionary processes through convergent evolution rather than gene …

The Effect Of Indaziflam On Seed Production In Perennial Cool-Season Grasses, Benson F. Israelsen

All Graduate Theses and Dissertations, Fall 2023 to Present

Annual invasive grass weeds are a major problem in grass seed production as they contaminate seed lots and decrease the quality. Few effective herbicides are available to control invasive grass weeds in seed production systems. Indaziflam is a pre-emergence herbicide capable of providing annual weed control for 3-5 years after application. Indaziflam is not labeled for use in seed production as little is known of the effect it will have. The purpose of this study was to measure total seed yield, seed germination, seed mass, and seedling vigor when applying 0, 40, 77, and 93 g ai ha^-1 of …

Assessing Khumbu Region Glaciers Mass Balance Change Using Geospatial Techniques From 2012 To 2024, Yashvi Jagatbhai Shah

Electronic Theses, Projects, and Dissertations

The glaciers in the Hindu Kush Himalaya are critical for sustaining the water supply of South Asia's major rivers, upon which over 230 million people, both in the mountainous regions and lowland areas, depend. Climate change is driving rapid glaciers melt near the summit of Mount Everest, located at an elevation of approximately 7,906 meters (25,938 feet). This has resulted in glacier thinning occurring up to 80 times faster than the original ice formation. However, due to their disposition in a complex topographic setting and inaccessible terrain, continuous glacier observations pose significant challenges.

This study evaluated the mass balance of …

Cognitive Differences In Parkinson’S Disease With Amyloid Positivity And Negativity, Kenya Luna

Electronic Theses, Projects, and Dissertations

In Parkinson’s Disease (PD), research has shifted to investigate how biomarkers commonly seen in Alzheimer’s Disease (AD), such as amyloid beta (AB), may be associated with cognitive functioning in PD. AB is considered a reliable biomarker for AD pathology, however in PD there is a lacking biomarker that can accurately reflect severity of cognitive impairment. AD research has shown an association between low AB and cognitive decline, but the data in PD has mixed results. Most studies that analyze cognitive decline and biomarkers do not use a cutoff level and the few that do have a threshold vary greatly in …

The Effect Of Gender Beliefs On Self-Determined Motivation As Measured By Organizational Citizenship Behaviors And Workplace Deviant Behaviors, Ryan Jaramillo

Electronic Theses, Projects, and Dissertations

Employees are gauged by their motivation to complete their required job responsibilities, as losses in motivation can result in losses to organizations. Understanding motivation is necessary to achieving beneficial organizational outcomes such as organizational citizenship behaviors (OCBs) while reducing detrimental employee outcomes such as workplace deviant behaviors (WDBs). To this end, the present study analyzes motivation not through a dichotomy of intrinsic and extrinsic motivation, but through a spectrum of self-determination for its relationship to employee behaviors. Gender is a potential moderator that can have important moderating effects between motivation and subsequent outcomes in the workplace. The purpose of this …

Comparative Assessment Of Machine Learning And Deep Learning Models For Drug Effectiveness Using Sentiment Analysis, Blessing Ogechukwu Nwogu

Electronic Theses, Projects, and Dissertations

In recent years, the proliferation of online patient-generated drug reviews has created a valuable resource for assessing drug effectiveness and patient satisfaction, with sentiment analysis emerging as a powerful tool for extracting insights from this unstructured data.

This culminating research project conducted a comparative analysis of traditional Machine Learning (ML) and Deep Learning (DL) models for assessing drug effectiveness using sentiment analysis of participant reviews. The research aimed to evaluate the performance of Support Vector Machine (SVM), XGBoost, Random Forest, Long Short-Term Memory (LSTM), and Bidirectional Encoder Representations from Transformers (BERT) models in this context. This culminating research project addressed …

Development Of An In Vitro Squamous Metaplasia Model Using Airway Basal Stem Cells, Arash Tahmasbi

Electronic Theses, Projects, and Dissertations

Squamous metaplasia (SM) of the airway epithelium is an abnormal condition that is caused by exposure to airborne toxicants, cigarette smoke, pollutants, and pathogens. Since SM of the airway was first documented in the 1970s, data on this epithelial disease remain limited. More research is needed to fully understand this epithelial disorder and to prevent its progression into severe illnesses such as chronic bronchitis (CB), COPD, and squamous cell carcinoma. We developed 3D models of SM using airway basal stem cells (ABSCs) isolated from lung obtained from postmortem human donors. The ABSCs were cultured at the air-liquid interface (ALI) for …

Exercise Therapy Rescues Skeletal Muscle Dysfunction And Exercise Intolerance In Cardiometabolic Hfpef, Heather Quiriarte, Robert C. Noland, James E. Stampley, Gregory Davis, Zhen Li, Eunhan Cho, Youyoung Kim, Jake Doiron, Guillaume Spielmann, Sujoy Ghosh, Sanjiv J. Shah, Brian A. Irving, David J. Lefer, Timothy D. Allerton

School of Graduate Studies Faculty Publications

Exercise intolerance, a hallmark of heart failure with preserved ejection fraction (HFpEF) exacerbated by obesity, involves unclear mechanisms related to skeletal muscle metabolism. In a “2-hit” model of HFpEF, we investigated the ability of exercise therapy (voluntary wheel running) to reverse skeletal muscle dysfunction and exercise intolerance. Using state-of-the-art metabolic cages and a multiomic approach, we demonstrate exercise can rescue dysfunctional skeletal muscle lipid and branched-chain amino acid oxidation and restore exercise capacity in mice with cardiometabolic HFpEF. These results underscore the importance of skeletal muscle metabolism to improve exercise intolerance in HFpEF.

The Impact Of In Ovo Administration Of Probiotic Lactobacilli On The Gut Health And Immune Competence Of Broiler Chickens, Shreeya Sharma

All Theses

Due to the incomplete development of their immune system, newly hatched chicks are vulnerable to a wide range of pathogenic microorganisms, including enteric bacterial pathogens during the early stage of life. To boost their immune system during this critical period, numerous studies suggest early feeding, either in ovo or immediately post-hatch, of nutrients and immunomodulators such as prebiotics, probiotics, phytobiotics, Toll-like receptors (TLRs) ligands, vitamins and minerals. Probiotics, defined as live, non-pathogenic microorganisms that confer beneficial effects on the host, have been extensively studied for their effects on poultry health. Supplementation of probiotics to chickens has demonstrated a broad spectrum …

Life Cycle Assessment Approach For Energy Usage In Animal Rendering Plants, Abel Binoy Mathew

All Theses

The animal rendering industry plays a crucial role in transforming waste products from livestock production and meat processing facilities into valuable and economically beneficial products, promoting both resource efficiency and sustainability. This study uses a life cycle assessment (LCA) approach to evaluate energy usage and greenhouse gas emissions in animal rendering plants, focusing on electricity and natural gas as the main energy inputs for plant operation. This research examines three distinct rendering facilities, each processing different raw materials, to quantify the environmental impacts of rendering operations. Site visits, data collection, and real-time monitoring were used to capture energy use across …

Field Prevalence Of Ring Nematodes In South Carolina And Its Potential To Predispose Peanut Pods To Aflatoxin Contamination, Sarala Giri

All Theses

Peanut ring nematode, Mesocriconema ornatum is a nematode pest of peanut which can cause necrotic lesions on pods, pegs, and roots. It has been reported to facilitate secondary infections in peanut and other crops resulting in more significant losses in yield and quality. Aspergillus flavus is a ubiquitous facultative saprophyte that produces aflatoxin, the most problematic mycotoxin for global health and economy. Experiments were conducted at the Edisto Research and Education Center of Clemson University to examine the relationship of ring nematode populations at pegging and harvest with aflatoxin concentration in peanut kernels due to infection by A. flavus …

An Examination Of The Effect Of The Implementation Of A Trauma-Informed School Initiative On The Attitudes Of The Staff In A School, Michael Riddell

Electronic Theses and Dissertations

The purpose of this qualitative phenomenological case study was to investigate the change in the attitudes of teachers pre and post implementation of a trauma-informed school initiative in a school in the Eastern Grand Region of Tennessee. As adverse childhood experiences (ACEs) have become more recognizable in school age children by teachers and school staff members due to an increase in knowledge about ACEs, there has been an increase in the number of trauma-informed initiatives undertaken by schools across the United States. Due to the challenges associated with the implementation of trauma-informed initiatives in schools, it is important for administrators …

Unraveling Phytosulfokine Trafficking In Arabidopsis Thaliana Using Fiber-Optic Fluorescence Microscopy, Issaka Obuaba

Electronic Theses and Dissertations

As sessile organisms, plants manage stress through complex signaling networks involving phytohormones such as phytosulfokine (PSK). PSK, a disulfated pentapeptide, regulates plant growth, development, and stress responses by interacting with specific PSK receptors (PSKRs). In this study, we explored the trafficking dynamics of PSK, its post-application fate, and the synthesis of an analog. We administered both native PSK and a fluorescent version tagged with TAMRA (5(6)-carboxytetramethylrhodamine) to various Arabidopsis thaliana genotypes, including wild type, a PSKR-deficient mutant, and a strain overexpressing PSKR1 tagged with green fluorescent protein (GFP) over the wild-type background. Fiber-optic fluorescence microscopy revealed that receptor presence influences …

Perceiving, Processing, And Evaluating Infant Facial Affect In Potential Parents: An Erp Study, Tiffany Bonnagio

Electronic Theses and Dissertations

Evidence for differences between assigned female at birth (AFAB) and assigned male at birth (AMAB) perceiving, processing, and evaluating infant faces has been mixed. The current study examined whether expected parental self-efficacy (EPSE) and desire for children predicts event-related potentials (ERPs) to infant facial affect in potential parents. No scale for EPSE exists. Therefore, we reworded the Perceived Maternal Parental Self-Efficacy scale from present to future tense. There was no correlation with EPSE scores, ERPs, or behavioral data. No difference was found between AFAB and AMAB in accurately evaluating infant faces; however, AFABs responded more quickly. ERP data revealed significantly …

Resilient Students: Traumatic Experiences, Aces, And Resources That Influence University Students’ Perceptions Of First-Year College Adjustment, Audrey W. Besch

Electronic Theses and Dissertations

The history of understanding the impacts of trauma on individuals has shifted from a narrow focus on physical injuries to a multidimensional understanding of how trauma influences the development of psychological and emotional interactions. This evolving understanding has informed the implementation of trauma-informed practices and interventions across various sectors. Tennessee institutions of higher education recognize the connection between trauma, student success, and systemic interventions. University students who have experienced Adverse Childhood Experiences have heightened vulnerability to stressful events as they transition from high school to college, increasing the likelihood of lower educational attainment, higher drop-out rates, and higher rates of …