Digital Commons Network^™

The Role And Therapeutic Possibilities Of Pelp1 In Hepatocellular Carcinoma (Hcc) Cells, Marian Hernandez Fernandez

Honors Program Theses and Research Projects

Hepatocellular carcinoma (HCC) is the most rapidly increasing cause of cancer mortality in the USA and ranks as the fourth leading cause of cancer-related deaths worldwide. Moreover, advanced HCC has a bleak prognosis, with a median survival rate of approximately 10 months. Latinos in South Texas exhibit the greatest incidence of liver cancer nationwide. Proline-, glutamic acid-, and leucine-rich protein 1 (PELP1) is an oncogene associated with the advancement of several cancers; however, its function in hepatocellular carcinoma (HCC) remains unclear. Analysis of publicly accessible cancer databases revealed elevated expression of PELP1 oncogene in HCC relative to the control group. …

Rhizobacterial Isolates From The Native Plant Ceanothus Velutinus Promote Growth In Two Genotypes Of Tall Fescue, Ananta Raj Devkota, Sukhmanjot Kaur, Amita Kaundal

Plants, Soils and Climate Student Research

Global warming-induced climate change haunts the world, posing a critical threat to plant health and crop production. Overusing chemical fertilizers and pesticides poses a significant threat to soil health. Ceanothus velutinus (snowbrush) is a drought-tolerant, actinorhizal native plant found in the Intermountain West region of the US that harbors many plant growth-promoting rhizobacteria (PGPR). In this study, we evaluated the effects of PGPR CK-06, CK-22, CK-44, and CK-50 from C. velutinus on the growth and development of two tall fescue genotypes: (i) a lawn-type tall fescue blend and (ii) an endophyte-free forage-type tall fescue known as Armory. Tall fescue plants …

Gardner-Webb Assistant Professor Dr. Lorell Gordon Celebrates Achievements Of Her Students, Office Of University Communications, Avery Copeland

Gardner-Webb NewsCenter Archive

Dr. Lorell Gordon, an assistant professor in the Gardner-Webb University School of Psychological Science and Counselor Education, recognized a need within the school’s program offerings and worked with her colleagues on a solution. She played a key role in developing and implementing the Marriage, Couples, and Family Counseling (MCFC) concentration within the Clinical Mental Health Counseling Program.

Teacher’S Perspectives Regarding Kindergarten Students’ Behaviors And Social Skills Post-Pandemic, Latalia Middlebrook Robbins

Walden Dissertations and Doctoral Studies

Kindergarten teachers and support professionals in the social-emotional community within a local school district experienced changes in student behaviors and social skills since returning to in-person schooling following the pandemic. After COVID-19, teachers found themselves engaging students who presented a lack of social skill development, contributing to the behaviors within the classroom microsystem. This study used Urie Bronfenbrenner’s ecological systems theory to support research efforts to examine how learning environments and interactions contributed to students’ preparation for Kindergarten. The research explored 12 Kindergarten teachers' and support professionals' perspectives concerning Kindergarten student behaviors and social skills. The research design was an …

Examining The Relationship Between Personality, Values, And Treatment Retention In Substance Abuse Programs: A Quantitative Correlation Analysis, Jonathan Ciampi

Walden Dissertations and Doctoral Studies

Substance Use Disorder (SUD) continues to be a global issue with devasting effects on the community, loved ones, and individuals suffering from SUD. Healthcare professionals continue to develop improved treatments for addressing patient needs, yet treatment abandonment remains high. While studies have examined treatment retention factors, few have examined the relationship between personality traits, values, and treatment retention. This study examined the relationship between the five-factor model of personality traits, the theory of formal axiology of values, and the days in treatment for adults in a telehealth substance abuse outpatient clinic in the United States. The study assessed the combined …

The Effect Of Variable Learning Contexts On Novel Word Retention In Adults, Emily A. Badoni

Theses and Dissertations

This study investigated the impact of decontextualized learning on memory recall and retention within virtual reality (VR) environments. Forty-six healthy young adult participants, both male and female, were randomly assigned to either a consistent VR learning condition (n = 23) or a diverse VR learning condition (n = 23). Both groups were exposed to the same set of 30 nonwords and their definitions across three learning phases. The participants in the consistent condition remained in the same VR environment throughout the study, while the participants in the diverse condition experienced three different VR environments. Immediate and delayed post-tests were conducted …

Physics-Informed Transfer Learning For Process Control, Samuel Arce Munoz

Theses and Dissertations

In the realm of process control, managing complex systems with limited prior knowledge presents significant challenges, particularly in environments where traditional mechanistic models are either unavailable or computationally prohibitive. This work explores the integration of deep transfer learning with system identification and Model Predictive Control (MPC) to develop control strategies that are both data-efficient and computationally streamlined. Initially, Long Short-Term Memory (LSTM) networks are employed to create approximate MPC controllers by leveraging transfer learning from a source system to a target system, demonstrating that transfer learning can achieve comparable performance to traditional MPC methods with reduced training data. Building upon …

Conditional Cooperation With Longer Memory, Nikoleta E. Glynatsi, Ethan Akin, Martin A. Nowak, Christian Hilbe

Mathematics and Statistics Faculty Research & Creative Works

Direct reciprocity is a wide-spread mechanism for the evolution of cooperation. In repeated interactions, players can condition their behavior on previous outcomes. A well-known approach is given by reactive strategies, which respond to the coplayer's previous move. Here, we extend reactive strategies to longer memories. A reactive-n strategy takes into account the sequence of the last n moves of the coplayer. A reactive-n counting strategy responds to how often the coplayer cooperated during the last n rounds. We derive an algorithm to identify the partner strategies within these strategy sets. Partner strategies are those that ensure mutual cooperation without exploitation. …

Expired Dexon Drug As Green Inhibitor For Carbon Steel In Simulated Seawater Medium, Shams Naji, Rana Anaee, Sadeer Majeed

Journal of Applied Sciences and Nanotechnology

The addition of expired drugs as green inhibitors takes high attention as an alternative material compared with other expensive, toxic, non-available chemical inhibitors. In this study, the Dexon drug was suggested as a green inhibitor for commonly used carbon steel in (3.5% NaCl solution) within a temperature range of 303 to 333 K. Corrosion/inhibition investigation was done by potentiostatic analysis to record the corrosion potential, corrosion current density, and Tafel slopes for the added four concentrations (4, 20, 40 and 60 mL/L) of the drug. The polarization resistances and inhibition efficiencies reached the highest value of (25.86×10-3 Ω.cm2) and (95.57%) …

Studies Of Media To Maximize The Expression Of Bacterial Metalloprotease Genes As Potential Therapeutics For Human Alzheimer’S Disease, Uday Kiran Chigurupati

Honors Capstones

This study investigates the potential of bacterial metalloprotease genes as therapeutic agents for Alzheimer's Disease (AD). The research focuses on Pseudomonas 20ei1 (P. 20ei1), a bacterial species isolated from lagoon water, which contains pitrilysin genes that share similarities with human insulysin. Six pqqL-related genes were identified in P. 20ei1 for this study. The research methodology involved bioinformatic studies, bacterial cell culture in various media, RNA extraction, purification, and cDNA synthesis. Quantitative PCR analysis was performed to analyze gene expression levels in different cDNA samples. The bacteria grown in the medium with insoluble phosphate has shown higher expression of selected genes …

Gardner-Webb Incoming First-Year Students Form Strong Bonds In Living-Learning Communities (Llc), Office Of University Communications

Gardner-Webb NewsCenter Archive

After successfully introducing Gardner-Webb University’s first living-learning community (LLC) last year, the Division of Student Success in collaboration with the Office of Housing and Residence Education added two more LLCs for the 2024 Fall Semester. Incoming first-year students could choose to participate in one of three LLCs: Faith, STEM or Honors.

Teacher’S Perspectives Regarding Kindergarten Students’ Behaviors And Social Skills Post-Pandemic, Latalia Middlebrook Robbins

Walden Dissertations and Doctoral Studies

Kindergarten teachers and support professionals in the social-emotional community within a local school district experienced changes in student behaviors and social skills since returning to in-person schooling following the pandemic. After COVID-19, teachers found themselves engaging students who presented a lack of social skill development, contributing to the behaviors within the classroom microsystem. This study used Urie Bronfenbrenner’s ecological systems theory to support research efforts to examine how learning environments and interactions contributed to students’ preparation for Kindergarten. The research explored 12 Kindergarten teachers' and support professionals' perspectives concerning Kindergarten student behaviors and social skills. The research design was an …

Regulatory Dynamics Of Arginine Metabolism In Staphylococcus Aureus, Itidal Reslane, Gabrielle F. Watson, Luke D. Handke, Paul D. Fey

Open Access Articles Funded by the University of Nebraska Open Access Publishing Agreements or the former UNO Open Access Fund

Staphylococcus aureus is a highly significant pathogen with several well studied and defined virulence factors. However, the metabolic pathways that are required to facilitate infection are not well described. Previous data have documented that S. aureus requires glucose catabolism during initial stages of infection. Therefore, certain nutrients whose biosynthetic pathway is under carbon catabolite repression and CcpA, including arginine, must be acquired from the host. However, even though S. aureus encodes pathways to synthesize arginine, biosynthesis of arginine is repressed even in the absence of glucose. Why is S. aureus a functional arginine auxotroph? This review discusses recently described regulatory …

Response Of Mucinous Breast Carcinoma To Neoadjuvant Chemotherapy, Berkay Kiliç, Burak İlhan

Turkish Journal of Medical Sciences

Background/aim: Mucinous breast carcinoma (MC) is thought to be a favorable-differentiated form of invasive breast cancer and is rarely preferred for neoadjuvant chemotherapy (NAC). The study aimed to define the response of MCs to NAC.Materials and methods: The study reviewed the demographic, clinicopathologic characteristics, management, and follow-up data of 70 patients diagnosed with MC between May 2010 to December 2020 by examining patients’ historical files and oncology records.Results: The median age, tumor size, and follow-up period of patients were respectively 52.9 (range 32 to 87) years, 25.8 (range 8 to 88) mm, and 61.5 (range 18 to 143) months. Out …

High Sacral Slope, Lumbar Lordosis, And Sacral Slope-To-Pelvic Incidence Ratio Are Associated With New Bone Formation In Ankylosing Spondylitis, Aysun Aksoy, Cemal Gündoğmuş, Mehmet Kesi̇mer, Kemal Nas, İkram Duman, Gazanfer Eki̇nci̇, Murat Bezer, Pami̇r Atagündüz

Turkish Journal of Medical Sciences

Background/aim: Syndesmophyte formation appears to be site specific in Ankylosing spondylitis(AS),and new bone formation seems to occur in regions of micro-trauma that are prone to tensile forces.Pelvic and spinal parameters are unique for individuals. Pelvic tilt and sacral slope are important anatomical features that compensate in harmony in keeping the sagittal balance. After puberty, the sacral slope shapes the lumbar lordosis, whereas the pelvic incidence is set to an individual constant. This study aims to analyze the properties of pelvic parameters in AS patients with and without syndesmophyte formation in the spine after 15years of disease duration.Materials and methods:Whole spine …

Lipid Discovery Enabled By Sequence Statistics And Machine Learning, Priya M. Christensen, Jonathan Martin, Aparna Uppuluri, Luke R. Joyce, Yahan Wei, Ziqiang Guan, Faruck Morcos, Kelli L. Palmer

School of Podiatric Medicine Publications

Bacterial membranes are complex and dynamic, arising from an array of evolutionary pressures. One enzyme that alters membrane compositions through covalent lipid modification is MprF. We recently identified that Streptococcus agalactiae MprF synthesizes lysyl-phosphatidylglycerol (Lys-PG) from anionic PG, and a novel cationic lipid, lysyl-glucosyldiacylglycerol (Lys-Glc-DAG), from neutral glycolipid Glc-DAG. This unexpected result prompted us to investigate whether Lys-Glc-DAG occurs in other MprF-containing bacteria, and whether other novel MprF products exist. Here, we studied protein sequence features determining MprF substrate specificity. First, pairwise analyses identified several streptococcal MprFs synthesizing LysGlc-DAG. Second, a restricted Boltzmann machine-guided approach led us to discover an …

Diverse Possibilities Of Shakespeare: Teaching Twelfth Night Through A Queer Lens, Julia Ritzler

Honors Projects

This project seeks to understand the ways in which Queer Theory can be applied to secondary language arts education to enhance students' understanding of identity and gender in literature. For the purposes of this project, Shakespeare’s Twelfth Night is being used as a case study text. This project includes a thorough analysis of Twelfth Night through a queer lens, a literature review of Queer Theory and educational pedagogy, a block plan and related instructional and assessment materials, a rationale for the block plan, and project limitations and implications for future research. By analyzing scholarly texts and applying the theories within …

Mapping Out, Hector Escobar, Erik Ziedses Des Plantes

University Libraries Blog Archive

On Nov. 20, Roesch Library hosted its first GIS Day, a global event promoting the use and understanding of geographic information systems.

Evaluation Of The Efficacy Of Transfer Energy Capacitive And Resistive Therapy In Patients With Knee Osteoarthritis, Özge Tezen, Emi̇ne Esra Bi̇li̇r, Öznur Uzun, Duygu Yaniktaş, Başak Şentürk, Evren Yaşar

Turkish Journal of Medical Sciences

Background/aim: The aim of present study was to investigate the treatment efficacy of transfer energy capacitive and resistive (TECAR) therapy, a modern application that has been increasingly used in recent years, compared to conventional physical therapy (CPT) applications in the treatment of knee osteoarthritis, according to the evaluation of clinical findings of patients.Materials and methods: A total of 54 patients, aged between 40 and 75 years, were randomly divided into two groups. Both groups received CPT. Group 2 received TECAR therapy in addition to CPT, applied three times a week for two weeks, for a total of 6 sessions. All …

Achillea Millefolium Ameliorates Doxorubicin-Induced Renal Injury Via Inhibition Of Oxidative Stress And Inflammation In Rats, Mohammed Shaiea, Yi̇mi̇ng Dong, Saleh Alomisy, Hassan Al-Mahbashi, Guozhong Zhang, Chuan Wang

Turkish Journal of Medical Sciences

Background/aim: Doxorubicin (Dox) is a potent anticancer medication. Nevertheless, due to nephrotoxicity, its clinical application is restricted. Achillea millefolium (AM) is a traditional medicine used against several disturbances, including kidney disorders. Therefore, this work aims to investigate the preventative properties of AM extract (AME) and their mechanisms against nephrotoxicity caused by Dox in rats. Materials and methods: The rats were assigned randomly to six groups, including a control group, Dox group (5 mg/kg/week via i.p. for 4 weeks), two groups, AME (100 or 200 mg/kg, orally for 28 days), and the last two groups, Dox+ AME (100 or 200 mg/kg, …

Lifetime Stressor Exposure Is Related To Suicidality In Autistic Adults: A Multinational Study, Rachel L. Moseley, Darren Hedley, Julie M. Gamble-Turner, Mirko Uljarević, Simon M. Bury, Grant Shields, Julian N. Trollor, Mark A. Stokes, George M. Slavich

Psychological Science Faculty Publications and Presentations

Despite very high rates of suicidal thoughts and behaviors (STB) in autistic adults, the key psychosocial drivers of this phenomenon remain unknown. To investigate, we examined how lifetime stressor exposure and severity, which have been found to predict STB in non-autistic populations, related to STB in a multinational dataset of 226 autistic adults from the United Kingdom and Australia (67% female; M_age = 41.8, SD = 13.6, range = 19–73 years old). Results revealed that autistic men and women differ with respect to the count, severity, and type of stressors they experienced over the life course. Whereas autistic men …

African Women Custodians: Teaching, Toponymy And Tacit Reverie In Heritage Education, Tokie Laotan-Brown

Wagadu: A Journal of Transnational Women's & Gender Studies

This paper investigates the critical role of women in African cultures as guardians of cultural heritage. It explores their central function in transmitting traditional ecological knowledge and practices across generations through established pedagogical methods, toponymic knowledge systems (place-names within cultural landscapes), and the cultivation of tacit knowledge. The paper emphasizes the significance of women's contributions to environmental education, often overlooked or undervalued within Western scientific paradigms. This marginalization restricts access to resources and participation in decision-making processes. It argues for the integration of traditional knowledge systems, informed by women's expertise, into formal education and pedagogy. This inclusion serves to validate …

The Effect Of Applied Mental Health Courses On College Student Anxiety And Perfectionism, Nathan Thomas Rhees

Theses and Dissertations

University students face a variety of mental health challenges, including increasingly high rates of anxiety. As demand for college counseling services among student populations rises, universities have an increasing need for effective and resource-efficient methods of intervention that support student mental health on their campuses. One area in need of research is that of using university classes themselves as a form of preventive intervention. If effective, this kind of preventive measure might be a particularly attractive method of reducing incidence of mental illness on campus and decreasing demand on university therapy resources. This study involves students at a private university …

Ai-Driven Anomaly Detection In Cybersecurity, Mohamed Almansoori

Theses

India has witnessed a huge level of digitization in the last two decades which has greatly impacted its economy and society. But the increasing use of technology has also intensified the number and level of threats to cyberspace in the country. Signature-based detection systems that have dominated the cybersecurity field for years have not been up to the challenge of modern attackers who use more complex and diverse attack methods. These challenges are well understood and this research focuses on improving the cybersecurity anomaly detection in India during the period between 2003 and 2022 using advanced AI and machine learning. …

Simple Improvements In Vector Design Afford Substantial Gains In Aav Delivery Of Aggregation-Slowing Aβ Variants, Ella Borgenheimer, Cameron Trueblood, Bryan L Nguyen, William R Lagor, Joanna L Jankowsky

Faculty, Staff and Students Publications

Adeno-associated virus (AAV) gene therapy for neurological disease has gained traction due to stunning advances in capsid evolution for CNS targeting. With AAV brain delivery now in focus, conventional improvements in viral expression vectors offer a complementary route for optimizing gene delivery. We previously introduced a novel AAV gene therapy to slow amyloid aggregation in the brain based on neuronal release of an Aβ sequence variant that inhibited fibrilization of wild-type Aβ. Here we explore three coding elements of the virally delivered DNA plasmid in an effort to maximize the production of therapeutic peptide in the brain. We demonstrate that …

Imlunestrant, An Oral Selective Estrogen Receptor Degrader, As Monotherapy And In Combination With Targeted Therapy In Estrogen Receptor-Positive, Human Epidermal Growth Factor Receptor 2-Negative Advanced Breast Cancer: Phase Ia/Ib Ember Study, Komal L Jhaveri, Cynthia X Ma, Et Al.

2020-Current year OA Pubs

PURPOSE: Imlunestrant is a next-generation oral selective estrogen receptor (ER) degrader designed to deliver continuous ER target inhibition, including in

METHODS: An i3+3 dose-escalation design was used, followed by dose expansions of imlunestrant as monotherapy or in combination with abemaciclib with or without aromatase inhibitor (AI), everolimus, or alpelisib. Imlunestrant was administered orally once daily and with the combination partner per label.

RESULTS: Overall, 262 patients with ER+/HER2- ABC were treated (phase Ia, n = 74; phase Ib, n = 188). Among patients who received imlunestrant monotherapy (n = 114), no dose-limiting toxicities or discontinuations occurred. At the RP2D (400 …

Prenatal Social Disadvantage Is Associated With Alterations In Functional Networks At Birth, Ashley N Nielsen, Regina L Triplett, Lourdes M Bernardez, Ursula A Tooley, Max P Herzberg, Rachel E Lean, Sydney Kaplan, Dominique Meyer, Jeanette K Kenley, Dimitrios Alexopoulos, David Losielle, Aidan Latham, Tara A Smyser, Arpana Agrawal, Josh S Shimony, Joshua J Jackson, J Philip Miller, Marcus E Raichle, Barbara B Warner, Cynthia E Rogers, Chad M Sylvester, Deanna M Barch, Joan L Luby, Christopher D Smyser

2020-Current year OA Pubs

Childhood exposure to social disadvantage is a major risk factor for psychiatric disorders and poor developmental, educational, and occupational outcomes, presumably because adverse exposures alter the neurodevelopmental processes that contribute to risk trajectories. Yet, given the limited social mobility in the United States and other countries, childhood social disadvantage is frequently preceded by maternal social disadvantage during pregnancy, potentially altering fetal brain development during a period of high neuroplasticity through hormonal, microbiome, epigenetic, and immune factors that cross the placenta and fetal blood-brain barrier. The current study examines prenatal social disadvantage to determine whether these exposures in utero are associated …

Angiogenin-Catalyzed Cleavage Within Trna Anticodon-Loops Identified By Cp-Rna-Seq, Megumi Shigematsu, Ryuma Matsubara, Justin Gumas, Takuya Kawamura, Yohei Kirino

Computational Medicine Center Faculty Papers

Angiogenin (Ang), an endoribonuclease belonging to the RNase A superfamily, cleaves the anticodon-loops of tRNAs to produce tRNA-half molecules. Although previous studies have demonstrated the involvement of Ang in the pathobiology of neurodegenerative disorders, the characterization of Ang-generated tRNA halves in neuronal cells remains limited. This is partly due to the technical limitations of standard RNA-seq methods, which cannot capture Ang-generated RNAs containing a 2',3'-cyclic phosphate (cP). In this report, we established an Ang-treatment model using SH-SY5Y, a human neuroblastoma cell line, and demonstrated Ang-dependent accumulation of tRNA halves. By performing cP-RNA-seq, which selectively captures cP-containing RNAs, we identified Ang-generated …

The Long Shadow Of Infection: The Long-Term Implications Of Herpes Simplex Virus And M. Tuberculosis Infection, Lydia Howell

Theses and Dissertations

Infectious diseases and autoimmune diseases, such as multiple sclerosis (MS) and type 1 diabetes (T1D), have traditionally been seen as distinct, with separate etiologies and courses of disease. Recent research has shown that infection and autoimmune disease are more closely woven together than previously thought; certain pathogens like Epstein-Barr virus and M. tuberculosis have been shown to cause long-term disruption to the immune system, for example (Bordignon et al 2011, #1) (Zhang et al 2023, #2) (Bjornevik et al 2023, #3). Conversely, a distinct pattern has emerged in developed countries where as rates of infection fall, rates of autoimmune disease …

Induction Of Tier 2 Hiv-Neutralizing Iga Antibodies In Rhesus Macaques Vaccinated With Bg505.664 Sosip, Justin C. Smith, Prabhu S. Arunachalam, Traci H. Legere, Lisa A. Cavacini, Eric Hunter, Bali Pulendran, Rama R. Amara, Pamela A. Kozlowski

School of Graduate Studies Faculty Publications

BACKGROUND: A goal of mucosal human immunodeficiency virus type 1 (HIV-1) vaccines is to generate mucosal plasma cells producing polymeric IgA (pIgA)-neutralizing antibodies at sites of viral entry. However, vaccine immunogens capable of eliciting IgA neutralizing antibodies (nAbs) that recognize tier 2 viral isolates have not yet been identified. METHODS: To determine if stabilized native-like HIV-1 envelope (Env) trimers could generate IgA nAbs, we purified total IgA and IgG from the banked sera of six rhesus macaques that had been found in a previous study to develop serum nAbs after subcutaneous immunization with BG505.664 SOSIP and 3M-052 adjuvant, which is …