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Prioritizing Amd Impaired Watersheds For Watershed-Scale Restoration Using Multi-Criteria Decision Analysis, James Nicholas Marino

Graduate Theses, Dissertations, and Problem Reports (ETD)

Acid Mine Drainage (AMD) remains a significant environmental challenge in coal-mining and hard rock regions like West Virginia, degrading water quality and ecosystem health. Traditional site-specific remediation efforts often prove inefficient in addressing AMD at large scale. This study explores watershed-scale restoration as a more effective solution, leveraging centralized treatment facilities and multi-source pollution management. This study will follow Multi-criteria Decision Analysis (MCDA), specifically the Analytical Hierarchy Process (AHP), to develop a structured rubric for grading watersheds. The process involves identifying critical criteria, organizing them into a hierarchy, and generating weights for clusters of criteria through collaboration with decision makers. …

Evaluating The Adaptation Of Taro (Colocasia Esculenta) To West Virginia Climatic Conditions, Kayode Joseph Ajayi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Climate change impacts agriculture and food security across the globe, therefore higher temperatures could increase in the length of the growing period and new crops would take advantages of. Research was conducted in Morgantown, WV in 2022 and 2023 to understand the growth pattern of Taro in West Virginia, and to analyzing the chemical composition of the Taro plant. The experiment was a completely randomized design in direct planting-low tunnel technology (2 replications, and 10- plants/plot), and two fertilization treatments (fertilized and non-fertilized). Spacing was 1 m within and between rows. Data were subjected to ANOVA using SAS. Dependent variables …

The Characterization Of Il-27 Production And Its Impact On T Cell Antigen-Specificity And Diversity In A Neonatal Bcg Vaccination Model, Ashley M. Divens

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tuberculosis (TB) is caused by Mycobacterium tuberculosis (Mtb), and it has plagued humans for most of recorded history. Despite strong efforts to prevent and treat TB, it remains the leading cause of death due to a pathogen and is most prevalent in certain regions of Asia and Africa. Currently, the only approved vaccine to prevent TB is the live-attenuated Bacillus Calmette-Guérin (BCG) vaccine. Most countries have a universal BCG vaccination policy, but the vaccine ultimately fails to be protective. The BCG vaccine is most effective in children and protects against disseminated TB, but protection wanes 10-15 years after vaccination causing …

Exploring Teacher Perception And Implementation Of Sae Programming In West Virginia, Joseph Laflamme

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study was to describe the phenomenon of SAE implementation in West Virginia. This study utilized qualitative research methodology to describe teacher perceptions of how student, guardian, school and community factors affect SAE program implementation. Additionally, teacher backgrounds, including formative experiences, training and education, guiding philosophies, skills, and motivation were explored. This study describes contextual aspects of population, economics, state guidelines and local agriculture industries. This is done through a document review of student reported records as well as surveys and teacher interviews.

Creative Energies: Unearthing The Role Of Romantic Ecocriticism, Nicole Marie Chambers-Stahl

Graduate Theses, Dissertations, and Problem Reports (ETD)

The literary field of ecocriticism has transcended the boundaries of Academia: excited to connect to their physical environments, many of these scholars are focusing on genres like place-based writing, rhetoric of energy policy, and representations of the Earth in film and media. Interdisciplinary by nature, ecocriticism has demonstrated clear potential in its ability to intersect with concentrations like the history of science, animal studies, and environmental justice.

However, as the language around global crises is heating up —“Today it is scientists, not prophets,” writes Tobias Menely in “‘The Present Obfuscation,’” “who speak the language of apocalypse” (478)—ecocritical scholarship is at …

Biomechanics Of Teeth Alignment Using Clear Aligners With Various Attachment Shapes And Orientations, Egon Mamboleo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Clear aligners have emerged as the most popular and preferred method of treatment for patients with orthodontic malocclusions. This is greatly due to the comfort and aesthetically appealing factors when compared to fixed appliances . Clear aligners are either thermoformed or 3D direct-printed plastics that apply biomechanical forces to the surface of teeth to trigger tooth movement and bone remodeling process. Common Class I malocclusions with mild or moderate crowding can be treated with clear aligners alone. However, treatment of Class II and Class III malocclusions that require extraction of permanent teeth or correction of severe rotations, and teeth extrusions …

Selective Recovery Of Critical Minerals (Ni, Zn, Co, And Mn) From Acid Mine Drainage Utilizing Ion Exchange And Selective Precipitation Processes, Esteban Albeiro Cardenas Restrepo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Critical minerals (CMs) are essential for U.S. national security and technological development. The U.S. heavily depends on imports of these minerals from other countries, making supply chain disruptions a likely scenario. In consequence, finding alternative sources to conventional mining for these elements has become a topic of interest in recent years. Acid Mine Drainage (AMD), once solely viewed as an environmental problem, has emerged as a potential alternative source for CMs. AMD is a naturally occurring process where surface and underground water interact with sulfur-bearing minerals in the presence of oxygen and microorganisms. This interaction causes the water to become …

Thermal Inactivation Of E. Faecium On Ground Poultry, Joseph Katz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Chicken products in grocery stores are contaminated with Salmonella that is ranked number one as a foodborne pathogen, and causes about 1.35 million cases of foodborne illness, 26,500 hospitalizations, and 420 deaths annually. The objective of this study is to determine thermal kinetic parameters of Salmonella surrogate bacteria E. faecium in ground poultry as affected by different temperatures and salt concentrations. The raw chicken was sliced up into a 500 g batch with a total of 6 batches per replicate, ground up in the meat grinder followed by inoculation with E. faecium resistant to 200 ppm Nalidixic Acid. NaCl …

Quantitative Assessment Of Knee Joint Stability Using Inertial Measurement Units In Patients With Acl Reconstruction Performing Single-Legged Hopping Tasks, Haley Hartman-Mongold

Graduate Theses, Dissertations, and Problem Reports (ETD)

Purpose: The purpose of this study was to examine proximal tibial IMU accelerations between the involved limb and uninvolved limbs of individuals who have undergone ACL reconstruction performing the single-leg hop test battery and to correlate these results to deficits in quadriceps strength and distance hopped. Methods: Thirty-four subjects (19 male, 15 female; age = 20.5 ± 6.7 years; mass = 76.4 ± 15.8 kg; height = 1.74 ± .08 cm) who have undergone unilateral ACL reconstruction were recruited for this study. An isokinetic dynamometer was used to assess the knee extension peak torque on the involved and uninvolved limbs. …

Academic Agency Within The Curricular Reform Of Gateway Stem Courses: A Faculty Perspective, Katie Beck

Graduate Theses, Dissertations, and Problem Reports (ETD)

The research regarding faculty agency during curricular reform is a burgeoning field within higher education as the faculty perspective within the current research concerning curriculum reform is limited. This study seeks to illuminate the faculty perspective of the curricular reform process of gateway STEM courses and how faculty experienced the reform process through a qualitative case study. The site of the case study is a public four-year institution on the eastern coast of the United States that has undergone and continues to undergo significant academic restructuring. The research utilized a structured survey and semi-structured interviews with seven total participants from …

Xanthine Oxidoreductase, Uric Acid, And Iron Homeostasis: Discovering A Therapeutic Vulnerability In Breast Cancer, Matthew G. Chapa

Graduate Theses, Dissertations, and Problem Reports (ETD)

Breast cancer remains the most frequently diagnosed malignancy and the leading cause of cancer related deaths in women. This is primarily due to distant metastases, which highlight the necessity to understand factors that contribute to breast cancer progression. Emerging evidence associates decreased tumor xanthine oxidoreductase (XOR) expression with a poorer prognosis, increased recurrence, and a more aggressive phenotype. While healthy liver, intestines, kidneys, and breast tissue have robust XOR expression and activity, there is a tendency for this to be ablated in carcinogenesis. This dissertation investigates XOR's role in breast cancer and identifies its product, uric acid (UA), as possessing …

Adolescent Attachment And Anxiety: Examining Implicit Emotion Beliefs And Emotion Regulation As Mediators, Jeanette Pool

Graduate Theses, Dissertations, and Problem Reports (ETD)

Anxiety is a growing issue in adolescents, with teens reporting worse and more severe symptoms in recent years (Kajastus et al., 2023; Solmi et al., 2022). There are several risk factors for developing anxiety symptoms, including cognitive, behavioral, and social factors. Effective emotion regulation (ER) has been shown to be helpful in decreasing anxiety symptom levels (Arbulu et al., 2023; Cho et al., 2019). A better understanding of precursors of ER could aid in prevention of anxiety. ER may stem from our personal beliefs about emotions (Ford et al., 2018; Schroder et al., 2015; Tamir et al., 2007) and to …

3d Printed Microfluidic Platforms For Elisa-Based Diagnostics: Advancing Toward Point Of Care, Brandi Marie Binkley

Graduate Theses, Dissertations, and Problem Reports (ETD)

Protein biomarker detection is a critical analytical method for quantifying proteins in biological samples. It is relevant not only in medical diagnostics and research but also numerous other fields such as environmental monitoring, pharmacology, food safety, and biotechnology. Sensitive detection at picogram-per-milliliter (pg/mL) levels is essential for diagnosing diseases, monitoring progression, and tailoring treatments, especially for low-abundance biomarkers like troponin, interleukin 6, carcinoembryonic antigen (CEA), activin A, and vascular endothelial growth factor (VEGF). Immunoassays, particularly enzyme-linked immunosorbent assays (ELISAs), are the gold standard for protein biomarker detection due to their high specificity and sensitivity. The development of point-of-care (POC) diagnostic …

Do Dung Beetles Sink Or Swim: Exploring Dung Beetle Response To Precipitation, Lauren T. Cheshire

Graduate Theses, Dissertations, and Problem Reports (ETD)

Global climate change constitutes a major threat to biodiversity and ecosystem services of many taxa. The Mid-Atlantic region of the US is expecting to see an increase in extreme precipitation events and flooding. Dung beetles (Scarabaeinae) can tunnel below the soil surface allowing for water to dissipate into the soil rather than sit on the surface and add to flooding conditions. They also can decrease methane emission by burying dung and reduce outbreaks of pests that prefer the moist environment of dung. They contribute roughly $380 million in ecosystem services annually in the United States, yet their services may be …

What Does It All Mean? Differences In Self-Defining Memories Between Community And Adjudicated Youth, Hannah Elias

Graduate Theses, Dissertations, and Problem Reports (ETD)

Identity development is driven in large part by self-defining memories, or memories though which we base our definition of who we are (Habermas & Bluck, 2000). In adolescence, cognitive skills are gained that allow us to make meaning, or learn lessons and gain insight, from self-defining memories (Bluck & Habermas, 2001; McLean & Thorne, 2003). To determine whether there are differences in their ability to make meaning from their self-defining memories, this study compares narratives of self-defining memories between community youth and adjudicated youth. Additionally, the ability to be specific in self-defining memory recollection may differ between these groups, which …

All-Terrain Vehicle Injuries Among Youth: A Multidimensional Analysis Of Injury Patterns In West Virginia And Safety Laws Across High-Fatality States, Sabrina Chyann Beal

Graduate Theses, Dissertations, and Problem Reports (ETD)

All-terrain vehicles (ATVs) pose significant injury risks, particularly among youth. These powerful machines are widely used across recreational and rural settings. West Virginia consistently ranks among the top five states for ATV-related deaths and injuries. Safety equipment and requirements are often not followed nor enforced, causing children and adolescents to be the most vulnerable group because they tend to not wear safety equipment such as helmets, ride on roadways, carry passengers, and operate oversized ATVS. This study examined ATV injury patterns from the WVU Medicine Trauma Registry and ATV laws across the five states with the highest reported fatalities. This …

Resilience In Adolescents With Type 1 Diabetes: The Relation Between Grit, Benefit-Finding, Diabetes Distress, Hba1c And Health-Related Quality Of Life, Hannah Hardiman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Type 1 diabetes (T1D) is a common pediatric chronic illness that requires complicated management by both the child and their caregiver. Research and clinical guidelines have highlighted adherence (as measured by HbA1c) and health-related quality of life (HRQOL) as important outcomes particularly in adolescents with T1D. Notably, diabetes distress has been identified as a risk factor contributing to poor adherence and HRQOL outcomes in this population. Given these considerations, understanding what resilience factors contribute to optimal outcomes is vitally important to inform future interventions. Grit and benefit-finding are two novel resilience factors that have yet to be fully evaluated …

Influence Of Different Rates Of Biochar And Deer Browsing On Pollinator Plant Productivity, Bipana Bhusal

Graduate Theses, Dissertations, and Problem Reports (ETD)

Biochar, a carbon-rich material produced from biomass, has received wide attention for soil amendment to improve plant growth. However, its specific effects on pollinator friendly plants remain poorly understood. In this study, we evaluated the effects of four biochar application rates (0, 10, 20, 40 tons/acre) and deer exclusion (fenced vs. unfenced) on the productivity of three pollinator species; Buckwheat (Fagopyrum esculentum), Plains Coreopsis (Coreopsis tinctoria), and Black-eyed Susan (Rudbeckia hirta) in a utility right of way with a history of mining. A split-plot randomized design was used, and the results were analyzed using …

Computational Approaches For Diagnosing Neurological Impairments And Restoring Functional Movement, Serhii Bahdasariants

Graduate Theses, Dissertations, and Problem Reports (ETD)

Movement is the primary means by which the nervous system converts intention into interaction with the environment. Voluntary limb motion enables standing, walking, reaching, and grasping—actions that underpin self‑care, communication, and economic participation. When diseases or injuries disrupt the pathways that couple neural commands to muscle forces—whether through cortical stroke, spinal cord injury, or peripheral nerve damage—mechanical outcomes such as weakness, loss of coordination, and ultimately reduced mobility emerge. These impairments cascade into secondary complications, increased dependence on caregivers, and diminished quality of life. Quantifying how the lesion alters the mechanics of movement is therefore essential for designing targeted interventions …

Engineering Nanomaterials From Phase-Change Biological Materials Reacting With Carbon Dioxide, Qingyang Li

Graduate Theses, Dissertations, and Problem Reports (ETD)

The current global situation concerning CO2 emissions is increasingly alarming, with rising concentrations of CO2 significantly worsening climate change and global warming. Atmospheric CO2 levels are now at their highest in millions of years, driving a host of catastrophic effects, including but not limited to extreme weather events, rising sea levels, ocean acidification, disrupted ecosystems, deteriorating air quality, respiratory health issues, heat-related illnesses, threats to food and water security, and escalating social and geopolitical tensions. To keep global warming to no more than 1.5°C higher than that of pre-industry – as called for in the Paris Agreement – emissions need …

A Limb-Speed-Driven Locomotor Control System And Its Ability To Adapt, Emily Marie Herrick

Graduate Theses, Dissertations, and Problem Reports (ETD)

Despite how simple walking may seem, the locomotor control system is structurally and functionally complex. Its hierarchical organization of supraspinal and spinal networks with forward and feedback pathways has many interactions at multiple levels that are dependent on the dynamics of a high-dimensional musculoskeletal system. Having a comprehensive understanding of sensorimotor integration within a healthy locomotor control system is crucial for understanding changes to the system due to age or neurologic disease and developing effective technologies to recover mobility in those populations. In this dissertation, we address persistent gaps in knowledge pertaining to how the nervous system controls locomotion.

In …

Expanding The Vibrating Sharp-Edge Spray Ionization Toolkit For Oligonucleotide Characterization, Sultan Mahmud

Graduate Theses, Dissertations, and Problem Reports (ETD)

A gentle or soft Ionization process is a critical component in native mass spectrometry (MS) to lift the biomolecules from the bulk solution into the gas phase environment of the mass spectrometer preserving their shape, topology and non-covalent interactions. Electrospray ionization (ESI) facilitates structural preservation of proteins, nucleic acids and their complexes and as a result it has been extensively used in native MS. However, in negative ion mode, ESI is somewhat limited in sensitivity due to corona discharge effect resulting from the requirement of a high amount of voltage. This often disrupts the ESI process and may not preserve …

Forensic Characterization And Comparison Of Modern Nail Polish Products By Their Physical Features And Chemical Composition, Madison Meredith Lindung

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nail polish can serve as valuable evidence in forensic investigations as it is often found on the hands and feet of individuals. Nail polish can break off and transfer easily during self-defense actions or forceful contact, such as in cases of kidnapping, sexual assault, or homicide. These residues can provide clues about who was at the scene, as well as where, when, and how the event evolved. However, compared to other types of paints commonly analyzed in forensic laboratories, limited forensic research has been conducted on the chemical composition and discrimination capabilities of popular nail products. The lack of information …

Efficacy Of Salmonella Surrogate Enterococcus Faecium In Poultry And Mash Broiler Feed, Corey Waitman Coe

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation investigates the efficacy of Enterococcus faecium as a surrogate for Salmonella in validating thermal inactivation processes during poultry feed manufacturing and meat processing. The research encompassed three studies evaluating microbial kinetics in mash broiler feed, reconstructed ground chicken, and predictive modeling approaches to improve food safety interventions. In the first study, the thermal resistance of nalidixic acid-resistant Salmonella Typhimurium and E. faecium was compared in mash broiler feed heated at 75–95°C. Results demonstrated that E. faecium exhibited significantly greater heat resistance than Salmonella, with longer shoulder times, greater tailing effects, and higher D-values, supporting its use as …

Neuroendocrine Regulation Of The Luteinizing Hormone Surge And Puberty Onset In Sheep, Max J. Griesgraber

Graduate Theses, Dissertations, and Problem Reports (ETD)

Gonadotropin-releasing hormone (GnRH), luteinizing hormone (LH), and follicle stimulating hormone (FSH) are essential for the reproduction of all mammalian species, including both sheep and humans. GnRH is secreted by hypothalamic neurons into the hypophyseal portal vasculature in a pulsatile manner, which then acts within the anterior pituitary to elicit the release of LH and FSH into wider systemic circulation. In females LH and FSH act on the ovaries to promote estradiol (E2) production, follicular development, and under certain conditions in regard to LH, ovulation. Furthermore, while E2 inhibits GnRH and subsequent LH secretion under most conditions, at high concentrations E2 …

Parental Behavioral Control Across Domains In Indian And Indian American Families, Avneet Batra

Graduate Theses, Dissertations, and Problem Reports (ETD)

Prior research literature has often found parental behavioral control to have mixed associations with varied adolescent outcomes (Bean et al., 2006; Finkenauer et al., 2005). However, a relatively lesser explored topic of research focusses on the domain specificity of this kind of control and its association with internalizing and externalizing behaviors in sample of Indian and Indian American parents (Arim et al., 2010). The current study adds to the limited literature by emphasizing the five domains of control (e.g., moral, conventional, prudential, personal and overlapping) based on the social cognitive domain theory and explores their association with adolescent adjustment outcomes …

Capillary Electrophoresis Analyses Using Ultraviolet Detection And Novel Mass Spectrometry Interfaces For The Characterization Of Small Molecules And Nanoparticles Of Pharmaceutical Interest, Yousef Shaaban Hassan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Capillary electrophoresis (CE) coupled to mass spectrometry (MS) offers a powerful analytical platform that unites high-efficiency separation with molecular-level identification. Despite its potential, limitations in interfacing and usability have constrained the widespread application of CE-MS. This thesis presents methodological advancements to enhance CE-based analysis through innovative interfacing, automation, and application to both small molecules and nanoparticle vaccine formulations. First, a nanoflow sheath CE-VSSI-MS interface is developed to overcome the limitations associated with flow-dependent ionization, enabling the use of narrow-bore capillaries, high separation voltages, and flexible pH conditions. This configuration effectively separated and detected diverse analytes, including β-blockers, amino acids, and …

Police Abuse In America And The Search For Remedy, Howard Ryan

Graduate Theses, Dissertations, and Problem Reports (ETD)

The dissertation contends that, despite many diligent efforts—from mass protests to a presidential task force—America is not yet on path to remedying its outsized patterns of police abuse. The abuse problem is broadly characterized herein through a “triad” of over-enforcement, excessive force, and racial discrimination. The Washington Post’s “Fatal Force” database, covering the years 2015 through 2024, is offered as a bellwether of American progress in addressing its police abuse; and, it is observed, the nation’s yearly count of fatal police shootings of civilians has been increasing, rather than decreasing.

To help make sense of our lack of national progress, …

From Sequencing To Conservation: Genomic Analysis Of Three Sportfish Species In West Virginia, Andrew Johnson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Situated in the Appalachian Mountains, one of the oldest mountain ranges on Earth, West Virginia waters boast rich ichthyofauna including native sportfish species walleye (Sander vitreus), largemouth bass (Micropterus nigricans), and muskellunge (Esox masquinongy). These three species are all native to the contemporary Ohio River watershed and play a major recreational and ecological role in local fisheries. Ecologically, all three species are apex predators and play a key role in ecosystems by directly influencing local fish assemblages. As dominant apex predators all three species are highly sought after in recreational fisheries with largemouth bass …

White Paper And Black Water: Environmental Control Of Labor In Appalachian Paper Mill Communities, Matthew C. Powell

Graduate Theses, Dissertations, and Problem Reports (ETD)

In several small Appalachian communities, the West Virginia Pulp & Paper Company dominated the local landscape. The company stripped the mountainsides of spruce trees for pulpwood and used rivers for industrial processes and waste disposal. The small towns along these rivers and between these mountains relied on paper mills for employment. Corporate control over the natural environment, and water more specifically, was a necessary requirement for paper production, but also acted as a source of power over employees and their communities. When paper workers sought to organize, the company responded by leveraging their control over the environment against them to …