Digital Commons Network^™

Genomic Epidemiology Of Staphylococcus Aureus Sequence-Type 97, Eva Armao

Honors Undergraduate Theses

Staphylococcus aureus (S. aureus) is a gram-positive bacterial pathogen that colonizes human nasal passages, mucous membranes, or the skin of carriers, with colonization commonly found in 20-30% of healthy people. S. aureus has the potential for two types of infection within human hosts: methicillin-susceptible (MSSA) and methicillin-resistant (MRSA). These infections can be further classified based on their route of infection, either known as community-associated (CA), healthcare-associated (HA), or healthcare-associated community-onset (HACO). Upon further investigation of infected populations, specific genetic subtypes of S. aureus, known as sequence types (ST), have been identified globally, with some exhibiting increased virulence among …

Uv- Altered Macrophages: Impact Of Uv Radiation On Phagocytic Receptor Expression In M2 Macrophages And Implications For Tissue Repair And Immunosuppression, Hala Hafez

Honors Undergraduate Theses

Ultraviolet radiation is an environmental stressor known to suppress immune function in the skin, but its effect on the immunoregulatory M2 phenotype macrophages is poorly studied. This study aims to investigate the impact of UV exposure on key markers of the M2 macrophage, which are critical for tissue repair and immunosuppression. THP-1 monocytes were differentiated and polarized into the M2 phenotype using Phorbol-12 myristate (PMA) and Cytokines IL-4 and IL-10. Once differentiation was confirmed, the macrophages were exposed to varying durations of UV radiation. Real Time Quantitative PCR was used to analyze expression changes in phagocytic receptors (CD206, CD163, Arg-1), …

Exploring Biostimulant Efficacy Under Limited Fertilizer And Water In Utah's Watermelon Production, Evan Christensen, Milena Oliveira, Prakriti Nepal, Youping Sun

Plants, Soils and Climate Student Research

The western U.S. has recently experienced some of the driest conditions on record, and Utah has been in a multi-year drought (2019-2023) that affected the agricultural sector, including vegetable producers. While vegetables are not a primary agricultural crop in Utah, they are still an important part of the horticultural economy with 763 farms growing 6,138 acres. As the fourth largest acreage vegetable crop in Utah (almost 10% of the acreage) watermelon and related crops in the gourd family Cucurbitaceae (nearly 36% of total vegetable acreage) are important to vegetable growers. Watermelon is a water-intensive crop and requires an adequate supply …

Biomarkers In Coronary Artery Disease: Systematic Review And Meta-Analysis, Nicola King, Neil A. Smart, Theodore Bungon, Martin Peacock, Shakil A. Awan

Faculty of Science and Engineering Datasets

Little information exists regarding the detection of early coronary heart disease protein biomarkers. The aim of this study was to investigate several potential candidates. Systematic review was carried out followed by meta-analysis. The standardized mean difference (95% confidence intervals) for each comparison was: Troponins 2.31 (1.18, 3.4), iL-6 1.3 (0.8, 1.81), fibrinogen 1.55 (1.16, 1.94), NT-proBNP 1.05 (0.72, 1.38), lipoprotein a 0.75 (0.48, 1.03) osteoprotegerin 0.92 (0.23, 1.62), VCAM-1 1.53 (0.87, 2.18), pentraxin 3 0.87 (−0.13, 1.87), PAI-1 2.51 (−0.65, 5.66) MMP9 1.25 (0.36, 2.14), MCP-1 1.99 (1.12, 2.85) and adiponectin −1.11 (−1.49, −0.73). Multiple biomarkers that could potentially be …

Whose Burden Is It Anyway? A Comprehensive Proposal To Reshape Food Safety Review By Treating Food As Medicine, Katya S. Cronin

GW Law Faculty Publications & Other Works

With more than sixty percent of U.S. adults struggling with at least one diet-related health condition, the relationship between nutrition and public health has never been clearer. Indeed, for the first time in over a century, food has a prominent place on the national political stage and is one of the exceedingly few issues that has garnered bipartisan support. The recent rise in popularity of "Food Is Medicine" initiatives, which seek to provide medically tailored or healthy meals to vulnerable populations, underscores the critical importance of food to public health. Yet, while "Food Is Medicine" is shifting the insurance, business, …

Foster Care Students And Their Success In Academic Achievements In School, Kirsten Nassiff

Theses and Dissertations

This applied dissertation was designed to provide better training and resources for teachers working with students in foster care who have experienced multiple foster care placements, and to assist them in achieving academic success.

The researcher conducted semi-structured interviews with six educators and staff at an alternative school that has the highest number of students in foster care, located in this school district. Using Braun and Clarke’s six steps for Thematic Analysis, each transcript was coded with the assistance of MAXQDA, and themes were developed.

An analysis of the data revealed that educators and staff do not receive adequate communication …

Analysis Of Mapt On Alzheimer’S Disease, Elaine Vanterpool, Marc Morgan Jr.

Student Posters

Alzheimer’s is the 7 th most common cause for death in humans and unfortunately has no cure. Coincidentally the gene MAPT (microtubule-associated protein tau) increases chances of obtaining Alzheimer's. The purpose of this study is to discover the mutation that resides within this gene. A website database called Simple Clinvar was used to generate information concerning Alzheimer’s disease and the different genetic variants associated with the disease. The SIFT algorithm was used to show whether the changes in amino acid sequences of the MAPT gene can affect protein function. PolyPhen was another prediction algorithm used to predict the influence that …

How Latine College Students Experience, Maneuver, And Navigate Within The Colonizing Notion Of Smartness, Jasmine Jetton-Gonzales

Theses and Dissertations

This study explores how Latine college students, particularly those who are the first in their family to attend college, navigate and maneuver within the dominating colonizing notion of smartness in academia. Rooted in an Anglo-centric educational framework, the American education system often marginalizes Latine students by devaluing their cultural and linguistic knowledge while upholding Eurocentric standards of intelligence. Using a qualitative critical research approach, this study employs testimonios to examine the lived experiences of Latine college graduates in Illinois. Findings reveal that Latine students frequently encounter systemic barriers that challenge their academic identity, including racialized perceptions of intelligence, institutionalized deficit …

Stress And Behavior In Beef Calves During Weaning, Kathleen E. Tierney

Theses and Dissertations

Animal welfare in commercial livestock operations has been an increasing topic of concern. It is well known that stress can have significant behavioral and physiological repercussions. It is especially important in commercial operations to reduce stress, as animal efficiency is the leading priority. In an effort to reduce stress on animals and improve welfare in livestock operations, several studies have evaluated common husbandry practices. One of the most widely studied practices is weaning, which encapsulates the process of removing the young from the dam. Several studies have evaluated the effects of weaning on beef cattle through a variety of behavioral …

The Impact Of Perfumes And Cosmetic Products On Human Health: A Narrative Review, Sharifa Alblooshi

All Works

Background: The use of perfumes and cosmetic products is widespread, serving personal hygiene, aesthetic, and olfactory functions. However, concerns have been raised regarding the potential health impacts associated with long-term exposure to various ingredients used in these products. Objectives: This narrative review aims to synthesize evidence on the health risks associated with perfumes and cosmetics, focusing on specific health concerns, including fertility, respiratory health, cancer risk, allergies, skin disorders, endocrine disruption, and neurological effects. It also discusses the presence of heavy metals in cosmetics, regulatory challenges, and the need for transparency in ingredient disclosure. Methods: A comprehensive review of the …

Fabrication Of Palladium Decorated Zinc Oxide Nanostructures For Non-Enzymatic Glucose Sensing, Reagan Aviha, Anju Joshi, Gymama Slaughter

Center for Bioelectronics Publications

The growing global burden of diabetes necessitates the development of glucose sensors that are not only reliable and sensitive but also cost-effective and amenable to point-of-care use. In this work, we report a non-enzymatic electrochemical glucose sensor based on laser-induced graphene (LIG), functionalized with zinc oxide (ZnO) and palladium (Pd) nanostructures. The ZnO nanostructures were systematically optimized on the LIG surface by varying electrochemical deposition parameters, including applied potential, temperature, and deposition time, to enhance the electrocatalytic oxidation of glucose in alkaline medium. Subsequent modification with Pd nanostructures further improved the electrocatalytic activity and sensitivity of the sensor. The performance …

Advanced Nanocomposite-Based Electrochemical Sensor For Ultra-Sensitive Dopamine Detection In Physiological Fluids, Megha Shinde, Gymama Slaughter

Center for Bioelectronics Publications

This study presents a novel point-of-care electrochemical sensor for dopamine (DA) detection, featuring a flexible laser-induced graphene (LIG) modified with a unique nanocomposite comprising Nb₄C₃T_x MXene, polypyrrole (PPy), and iron nanoparticles (FeNPs). The LIG-Nb₄C₃T_x MXene-PPy-FeNPs is characterized by scanning electron microscopy to confirm the successful surface modification. The electrochemical performance of the fabricated sensor via cyclic voltammetry showed significant electrochemical activity upon Nb₄C₃T_x MXene-PPy-FeNPs nanocomposite modification of the LIG surface with an increased peak anodic current (Ipa) from 43 μA to 104 μA. The sensor …

Electrochemical Strategies For Microrna Quantification Leveraging Amplification And Nanomaterials: A Review, Alexander Hunt, Gymama Slaughter

Center for Bioelectronics Publications

MicroRNAs (miRNAs) are small, non-coding RNAs that regulate gene expression and have emerged as critical biomarkers in various diseases, including cancer. Their stability in bodily fluids and role as oncogenes or tumor suppressors make them attractive targets for non-invasive diagnostics. However, conventional detection methods, such as Northern blotting, RT-PCR, and microarrays, are limited by low sensitivity, lengthy protocols, and limited specificity. Electrochemical biosensors offer a promising alternative, providing high sensitivity, rapid response times, portability, and cost-effectiveness. These biosensors translate miRNA hybridization events into quantifiable electrochemical signals, often leveraging redox-active labels, mediators, or intercalators. Recent advancements in nanomaterials and signal amplification …

Determining The Effects Of Light, Oxygen Diffusion, And 4-Hydroxy-2-Nonenal On Redox Stability Of Beef And Pork Oxymyoglobins, Rishav Kumar, Surendranath P. Suman, Runnan Li, Morgan M. Pfeiffer, Gretchen G. Mafi, Ranjith Ramanathan

Animal and Food Sciences Faculty Publications

Retail light conditions influence consumers’ perception of meat color. Previous studies have shown that lipid and myoglobin oxidation are interrelated. 4-Hydroxy-2-nonenal (HNE)-induced beef and pork oxymyoglobin (OxyMb) oxidation is influenced by pH, temperature, and atmospheric oxygen partial pressure. However, limited knowledge is available on the effects of HNE on myoglobin redox stability under light/dark and aerobic/anaerobic (either allow or not allow atmospheric oxygen in contact with myoglobin solution) systems. Therefore, the objective was to examine the effects of light and dark storage conditions (experiment 1) as well as to compare aerobic and anaerobic systems (experiment 2) on HNEinduced beef and …

Artificial Raising On Milk Replacer And Finishing Diet Did Not Affect Color And Oxidative Stability Of Longissimus Lumborum Muscles From Polypay Ram Lambs, Koushik Mondal, Surendranath P. Suman, Katherine G. Purvis, Gregg Rentfrow, Donald G. Ely, Brittany E. Davis, Jennifer Weinert-Nelson, Yifei Wang, Ana Paula A.A. Salim

Animal and Food Sciences Faculty Publications

Lamb production traits are affected by pre-weaning management (such as artificial raising on milk replacer) and finishing (forage vs. concentrate) strategies. The objective of the current study was to examine the effects of pre-weaning management and finishing systems on the color and oxidative stability of longissimus lumborum (LL) muscles from ram lambs. Polypay ram lambs were raised conventionally with ewes (CR; n = 10) or artificially on milk replacer (AR; n = 10). After weaning at 60 d, ram lambs in AR and CR were equally divided and randomly allocated to finishing on a high-forage (50:50 forage:concentrate; HF) or a …

Effects Of Feeding Varying Levels Of Mycotoxin-Containing Corn Fines On Diet Choice And Growth Performance Of Nursery Pigs, Duncan B. Paczosa, Tyler B. Chevalier, Sunday A. Adedokun, Lan Zheng, Merlin D. Lindemann

Animal and Food Sciences Faculty Publications

Mycotoxins in feed are known to negatively affect growth and other physiological processes in pigs. Two experiments were conducted to investigate the effects of feeding diets with varying levels of mycotoxins and boron (a nutrient reported to mitigate some aspects of mycotoxicosis). Screenings from the 2020 crop year corn contained mycotoxin levels of 23,038 ppb total fumonisins (FUM), 1,446 ppb zearalenone (ZEA), and 5,032 ppb total deoxynivalenol (DON). The corn fines were added to a corn-soybean meal diet formulated to meet or exceed NRC (National Research Council. 2012. Nutrient requirements of swine. 11th Revised Edition. Washington, DC: The National Academies …

Evaluation Of Increasing Levels Of Mycotoxin-Containing Corn Fines And Mitigants On Nursery Pig Growth Performance, Duncan B. Paczosa, Tyler B. Chevalier, Sunday A. Adedokun, Lan Zheng, Merlin D. Lindemann

Animal and Food Sciences Faculty Publications

The effects of feeding corn fines (screenings) containing mycotoxin levels greater than the FDA guidance (fumonisins) and advisory (deoxynivalenol) levels were evaluated using 150 crossbred pigs (initial BW: 6.42 ± 0.06 kg; 90 barrows and 60 gilts) in an 8-wk study by adding contaminated corn fines to create six diets. The corn fines used contained prestudy analyzed mycotoxin levels of 20,334 ppb total fumonisin, 1,499 ppb zearalenone, and 5,075 ppb total deoxynivalenol. The corn fines were added into a corn-soybean meal basal diet at 0%, 20%, 40%, and 60% corn fines (Diets 1 to 4, respectively). Diet 5 was created …

Stream Response To A Dam Removal: Mill Creek, Davenport, Ca, Jessica J. Williamson

Master's Theses

Dams have many effects on stream morphology and ecology, including a loss of sediment supply downstream, reduced access to the entire stream for aquatic fauna, and a flooded area upstream of the dam. Dams are a hurdle for migrating organisms such as anadromous fish, which is a group of concern. An over 100-year-old dam on Mill Creek, a stream draining a coastal watershed in central California, was removed in the fall of 2021. The site is in the Santa Cruz Mountains, where endangered salmonids such as steelhead, Oncorhynchus mykiss, have historically lived. To study the change in the stream from …

Separation And Surface Examination Of Spacecraft Cabin Particulates, Grace A. Belancik

Master's Theses

Particulate matter in the atmosphere is a known detriment to human health, but several factors affect any particulate’s particular toxicity including particle size, surface area, and chemical composition. Extensive studies have been conducted to examine particulate hazards on Earth, but in crewed spacecraft, like the International Space Station (ISS), the particulate environment is wholly unique due to both the controlled environment and lack of gravity. Atmospheric sampling studies are underway, but another convenient source of airborne particulates for analysis is the contents of the vacuum bags used by the astronauts to clean the air vents. However, no method currently exists …

Resilience After Prenatal Substance Exposure: Exploring The Role Of Cumulative And Specific Protective Factors In Predicting Toddlerhood Temperament Profiles, Anjeli Elkins

Theses and Dissertations--Psychology

Objective

Prenatal exposure to common substances, including nicotine, marijuana, cocaine, methamphetamine, opiates, and alcohol, has been linked to a wide range of psychiatric disorders and neurodevelopmental impairments. Identifying protective factors that may improve temperament profiles in toddlers who have experienced prenatal substance exposure may protect against these adverse outcomes and is crucial for developing targeted early interventions.

Methods

Participants (n=92; Mage = 28.7 years; 47.0% White) were biological mothers of toddlers (Mage = 22.6 months) with prenatal substance exposure. Mothers responded to an online survey that gathered information on substance use during pregnancy, proposed perinatal protective factors, and child temperament. …

"We Talk About It At The Dinner Table": Parent Discussions Of Identity And Politics In Diverse Adoptive Families In The United States, Abigail L. Moore

Theses and Dissertations--Psychology

Parents discussing identity and politics with their diverse adolescents can be protective against experiences of political stress and negative outcomes (Anderson, 2023). Identity discrepancies between parents and adolescents may complicate these conversations (McCrimmon et al., 2024). However, this research often only focuses on only one identity (e.g., race or gender; Mendez, 2022). Because negative political climates affect multiple identities simultaneously (Kassing et al., 2021), the current study explores political conversations in diverse, adoptive family contexts with varying (un)shared identities. Through reflexive thematic analysis (Braun & Clarke, 2021), 7 major themes were developed. Results suggest that identity discrepancies may underlie a …

Associations Between Queer Parent Family Socialization And Adolescent Outcomes Among Diverse Lgbtq+ Parent Families In The U.S., Nita U. Kulkarni

Theses and Dissertations--Psychology

Queer parent family socialization refers to the way LGBTQ+ parents talk with their children about what it means to be queer in society, how to handle discrimination, and ways to have pride in their family’s identity. This construct has been linked with positive well-being outcomes in children, and this study used a strengths-based, mixed- methods framework informed by Minority Stress Theory and intersectionality to examine how queer parent family socialization related to several adolescent well-being outcomes, namely internalizing behaviors, social competence, and adaptive coping. Participants recruited across two projects were 191 queer parents with an adolescent and 114 adolescents with …

Weighted Family History Density Of Substance Use: Influence On Participant Substance Use Onset, Escalation, And Duration, Carleigh A. Litteral

Theses and Dissertations--Psychology

Substance use disorders impose a staggering toll on the United States (U.S.), straining healthcare systems, destabilizing communities, and profoundly impacting both individuals and families. Approximately one in six Americans aged 12 and older had a past-year substance use disorder in 2022 (Key Substance Use, 2022), and nearly one-third of adults experience a substance use disorder in their lifetime (Wu, 2010), contributing to an economic burden exceeding $400 billion annually (National Drug Threat Assessment, 2011). Family history of substance use disorders is a key predictor of vulnerability, with individuals who have affected relatives being 2–8 times more …

Exploring The Effects Of Transcranial Direct Current Stimulation Of The Right Inferior Frontal Gyrus On Inhibitory Control In Posttraumatic Stress Disorder, Colton Rippey

Theses and Dissertations--Psychology

Background: The right inferior frontal gyrus (rIFG) is implicated in stop signal reaction time (SSRT) performance – a common measure of inhibitory control – and emotional expression and control. Individuals with posttraumatic stress disorder (PTSD) exhibit poorer stop SSRT performance, which is associated with rIFG hypoactivity. Neuromodulation of the rIFG with transcranial direct current stimulation (tDCS) can significantly improve inhibitory control and reduce fearful responding to threatening stimuli. Research has yet to test the effects of multifocal tDCS targeting the rIFG on inhibitory control or emotional reactivity in individuals with PTSD.

Methods: The current study aims to examine the impact …

Relationship Between Anticipated Stress And Individuals’ Health-Related Behaviors, Julie Parsons

Theses and Dissertations--Psychology

Prior work in assessing the relationship between stress and health behaviors has identified the complex and multi-directional nature of these relationships. However, minimal work has assessed the possible relationships between stress anticipation and health behaviors such as diet, physical activity, and sleep outcomes in individuals. This work seeks to address this gap by conducting a daily diary study assessing within-person associations between stress anticipation and health behaviors over the course of a week. We hypothesized that daily stress anticipation would be negatively associated with dietary scores, physical activity engagement reports, sleep quality, and sleep duration while being positively correlated with …

Interparental Conflict And Sibling Relationship Quality In Emerging Adulthood: An Emotional Security Perspective, Courtlyn A. Fields

Theses and Dissertations--Psychology

Sibling relationships are among the earliest lifelong relationships and have been found to be protective against family dysfunction and positive adjustment outcomes. Yet research on sibling relationships remains limited. The current study aims to expand understanding of sibling relationship quality in emerging adulthood through the application of Emotional Security Theory (EST). EST provides a framework for understanding how adult sibling relationships may be impacted by childhood experiences of destructive interparental conflict. A sample of 1222 undergraduates with siblings completed an online survey. Participants retrospectively reported on interparental conflict and emotional security in childhood, and reported on their current emotion regulation, …

Why Are Women Tired? Examining Sex-Dependent Effects Of Behavioral Rhythms On Perceived Sleepiness, Maricel Schuler

Theses and Dissertations--Psychology

Sleep complaints in women emerge at menarche and coincide with reproductive hormonal fluctuations throughout the monthly menstrual cycle. To our knowledge, we are the first study (N = 44) to utilize a validated algorithm that uses actigraphy-derived light and activity data in a novel approach to estimate circadian phase, referred here as the photobehavioral phase. We hypothesize that women will have earlier photobehavioral phases, larger phase angles, poorer perceived sleep quality, and greater sleepiness compared to men. In addition, we hypothesize that the perimenstrual phase will be associated with poorer sleep within a subsample of women (N = 15). Contrary …

Examining Values In The Treatment Of Borderline Personality Disorder: A Single-Case Experimental Design, Nicole Stumpp

Theses and Dissertations--Psychology

Borderline personality disorder impacts roughly 1.6% of the general population yet only a quarter of people with BPD are able to access treatment. Understanding the mechanisms that promote behavior change in treatment is crucial for improving care for this population. Experiential avoidance has been linked to risky behaviors, a prevalent and debilitating symptom observed in BPD. Values identification may be a fruitful avenue for engaging experiential avoidance in treatment. The goal of the present study is to understand the unique and combined effects of values identification and behavior change skills (i.e., opposite actions, exposure) on experiential avoidance (target), maladaptive behavior …

Randomized Controlled Trial Of A Single-Session Mechanism-Focused Intervention For Suicidal Thoughts And Behaviors, Martina Fruhbauerova

Theses and Dissertations--Psychology

Suicide remains a critical public health concern, with increasing rates and millions experiencing suicidal ideation annually. Although evidence-based treatments exist, many are time-intensive, underutilized, and often inaccessible to high-risk individuals, especially those recently discharged from inpatient psychiatric care. Brief, scalable interventions targeting core psychological mechanisms implicated in suicidal thoughts and behaviors (STBs) are needed. This study evaluated a novel Single Session Mechanism-Focused Intervention (SSMFI) designed to reduce hopelessness and negative perceptions of social relatedness among adults hospitalized for STBs. In a randomized controlled trial (N = 38) conducted on two inpatient psychiatric units, participants were assigned to either the SSMFI …

Utilizing Evisits To Increase Access To Genetic Services For The Hearing Loss Population: A Tailored Service Delivery Model, Ansley Roberts

Theses and Dissertations

Purpose: Patients referred for genetics services have specific barriers decreasing accessibility, such as communication difficulties, long wait times, and misconceptions about the utility of genetic testing. We assessed a new tailored service delivery model used by the Greenwood Genetic Center to evaluate patients referred for hearing loss to determine if this model increases access to genetics services for individuals with hearing loss. Methods: Data points such as wait times, testing plan, and diagnostic yield were compiled from patient medical records. Comparison and analysis of data was completed by visit type, in-person, virtual, and electronic visits (eVisits), between October 2023 – …