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Evaluating Yield And Quality Of Heat-Tolerant Broccoli Varieties For Rhode Island Farmers, Calla Pederson

Open Access Master's Theses

In the United States, broccoli is a popular vegetable, important for its nutrition and economic contributions alike. However, its future production is at risk as broccoli prefers cooler climates, with an optimal temperature range of 15 - 23°C (59 - 73°F) for growth, especially in the weeks leading up to harvest. Temperatures above this threshold lead to failure to develop, damage, and unmarketability. There has also been an emphasis on bringing broccoli production back to the East coast from California, as climate events threatened Californian agriculture and local food has become popular, necessitating outside of season broccoli production during times …

Investigation Of Csld And Pme Genes In Cell Division And Expansion In Physcomitrium Patens, Lia Bozza

Open Access Master's Theses

Cellulose Synthase-Like Ds (CSLDs) are cellulose synthase known to participate in cell plate formation and cell wall remodeling. In the moss model, Physcomitrium patens, double csld2/6 knockout (KO) mutants were previously shown to have gametophore leaf phenotypes that are consistent with defects in cytokinesis, including irregular cell shape, cell separations, and incomplete midrib formation (Wu et al., 2023a). When these mutants were grown on ammonium-free medium to promote gametophore development, the severity of the phenotype was more extreme, including incompletely formed cell plates and multinucleated cells (A. Roberts, personal communication). It was also reported that expression of several enzymes …

Black Women, Bold Science: Navigating Race, Gender, And Identity In Science, Mia L. Brown

Theses and Dissertations--Science, Technology, Engineering, and Mathematics (STEM) Education

This research study examines the challenges Black women encounter when in predominantly white, male academic and professional science settings, where they experience dual discrimination because of their race and gender. These biases perpetuate the negative stereotype that Black women are less competent than white men. This mixed methods study examines how Black women navigate their race, gender, and science identities within predominantly white institutions through their doctoral programs and professional science careers. The study implements Critical Race Mixed Methodology to combine survey data for a wide reach with interview data for detailed exploration. The study involved eight Black women science …

Navigating Stress In Higher Education Leadership: The Role Of Mindful Meditation For Women Administrators, Christine T. Harper

Theses and Dissertations--Educational Leadership Studies

Women higher education administrators encounter distinct stressors which can significantly impact wellbeing and lead to burnout. These challenges include implicit bias, difficulty achieving work-life balance, and increasing institutional expectations. This study utilizes a Mixed Methods Action Research (MMAR) framework to assess stress levels, trait mindfulness, coping mechanisms, and the effectiveness of a structured mindfulness-based intervention. While the quantitative analysis revealed only a slight increase in trait mindfulness and no notable reduction in stress levels, the qualitative findings showcased meaningful personal benefits to the participants. They reported heightened awareness of stress triggers, improved emotion regulation, and an ability to apply the …

Magnetic Heating Efficiency Of Magnetite Nanoparticles: Dependence On Size And Alternating Magnetic Field, Sarah E. Kubican

Theses and Dissertations--Biomedical Engineering

Magnetic heating mediated by magnetic iron oxide nanoparticles (MION) has several clinical applications including cancer thermal therapy, tissue thawing for organ preservation, and magnetogenetics. However, current models of magnetic heating, including the widely used linear response theory, inadequately predict MION heating efficiency. An experimental study covering alternating magnetic field (AMF) strengths and frequencies ranging from 3.98 – 27.85 kA/m and 103.6 – 984.1 kHz, respectively, with a range of MION core diameters 9.5- 30.3 nm was conducted to elucidate the relationships between these AMF parameters and MION sizes and the resulting heating efficiencies. Heating efficiency was found to increase approximately …

Pathogen Surveillance Of Viruses And Antimicrobial Resistance Through Wastewater Based Epidemiology, William Strike

Theses and Dissertations--Biomedical Engineering

Pandemic surveillance is a cornerstone in public health safety, as it enables identification of pathogens circulating within a community before they become widespread outbreaks. Wastewater based epidemiology has blossomed in recent years due to its success in the SARS-CoV-2 pandemic, but this form of public health surveillance has a global equity problem. A large majority of the wastewater-based surveillance work has been done in large metropolitan cities with access to advanced testing infrastructure, which poses a challenge as we consider where pandemics have historically emerged. Therefore, there is a need to identify, adapt, and develop workflows to meet the needs …

Stable Oxygen And Hydrogen Isotopic Ratios Of Precipitation And Modelling Of Precipitation Sources In Central Kentucky, Usa, 2017-2022, Ariel Gold-Mccoy

Theses and Dissertations--Civil Engineering

Our goals were to carry out analyses of a multi-year δ¹⁸O and δ²H dataset of precipitation from central Kentucky and to analyze the source of precipitation for storm events from 2017 to 2022. δ¹⁸O and δ²H were measured for 244 weekly precipitation samples collected by the National Atmospheric Deposition Program at Mackville, Kentucky, USA. Modelling precipitation source was carried out using HYSPLIT model for 414 events. Results of δ¹⁸O and δ²H from central Kentucky show: consistent seasonality, including summer highs and winter lows for δ¹⁸O and δ²H; correlation with air temperature; and …

No Hope?: Queer Negativity, Afropessimism, And The Limits Of Futurity, Colton Barton

Theses and Dissertations--English

Since their inceptions, black and queer studies have sought to articulate the positions of “black” and “queer” within American social life—often converging on the goal of liberation. This thesis examines the intersection of two “negative” theoretical frameworks—Afropessimism and anti-social queer theory (or “queer negativity”)—to interrogate both the limits and generative potential of radical negativity. Though emerging from distinct intellectual traditions, these frameworks conceptualize blackness and queerness as constitutive negations—gaps within the dominant social order that render those so marked inassimilable to normative notions of humanity. While the convergences of these theories can be illuminating, their shared pessimism risks obscuring alternative …

Fatal Futures: Slow Violence, Utopia, And The Postnatural Human In Contemporary American Literature, Shelby Roberts

Theses and Dissertations--English

Fatal Futures: Slow Violence, Utopia, and the Postnatural Human in Contemporary American Literature investigates the ways in which ideas of futurity are articulated in narratives that conceptualize both the end of the world and post-calamity society through the lens of a pessimistic, queer approach to understanding the future. The purpose of this project is to not only build previous research in the configurations of the body in queer, gothic, and speculative American writing as a whole, but to enter into a developing conversation surrounding Appalachian literature through the idea of kinship (the broadening of relationships both chemical and postnatural) as …

Geochemical And Sedimentological Signals Of Bottom Water Oxygen Dynamics From Lake Tanganyika (Zambia), Njahi Mwangala

Theses and Dissertations--Earth and Environmental Sciences

Lake Tanganyika is a large, ancient freshwater body with exceptional biodiversity that is located in the East African Rift System. Lake Tanganyika’s size and longevity make its sedimentary record useful for understanding environmental changes that cross spatial and temporal boundaries. Lake Tanganyika is known to be susceptible to global warming given its tropical location. Over 12 million people living within Lake Tanganyika’s watershed rely on fishing for their income and nutrition. Changes to limnological function driven by climate could therefore have serious implications for human health and biodiversity conservation. This study focuses on using geochemical properties of modern lake bottom …

Late Holocene Paleolimnology Of Lake Tanganyika (East Africa), Leandro Domingos Luz

Theses and Dissertations--Earth and Environmental Sciences

Understanding the long-term responses of ecosystems to climate variability is crucial for predicting and mitigating future changes in the Afrotropics, a region where a significant portion of the population is highly vulnerable to extreme climatic events. Lake Tanganyika, the largest rift lake in East Africa, offers a unique sedimentary archive that can be used to investigate the limnological changes, hydroclimatic shifts, and vegetation dynamics across various timescales. A multi-proxy approach was employed to assess the landscape responses to hydroclimatic fluctuations in the Late Holocene, integrating paleoenvironmental records from radiocarbon-dated sediment cores. This approach involved sedimentology, mineralogy, geochemistry, phytolith analysis, and …

Multi-Scale Analysis Of Karst Aquifer Vulnerability To Climate Change And Contamination Processes, Shishir Sarker

Theses and Dissertations--Earth and Environmental Sciences

Karst aquifers represent some of the world’s most productive yet vulnerable groundwater systems, supplying freshwater to approximately 10% of the global population across 15% of Earth’s ice-free land surface. Their distinctive hydrological characteristics— including rapid recharge, preferential flow paths, and limited natural filtration— create unique vulnerabilities to both climate variability and contamination that pose significant challenges for sustainable water resource management. This dissertation presents a comprehensive multi-scale analysis of karst aquifer vulnerability through three interconnected studies that examine climate change impacts, microbial contamination dynamics, and contaminant transport processes across diverse geographic and hydrogeologic settings.

Chapter two investigates climate change impacts …

How To Eat Right And Why We Ask That Question, Heather N. Carpenter

Theses and Dissertations--Gender and Women's Studies

What does it mean to “eat right?” The notion is, of course, culturally, historically, nationally, politically, ethnically, and class determined. For the purposes of this project, however, I am particularly interested in changing notions of what it means to “eat right” within the post-World War II to the early new millennium American context, as such notions are represented within the prescriptions of popular culture. I turn to popular culture because this is the commons of the hegemonic call to “normalcy”—this is where we all learn to imitate, aspire, or rebel against a generic American consumer-self in conformity with the biases, …

Plastic Gerontologies: Epigenetic Clocks As Instruments Of The Profitable Medicalization Of Aging, Hans T. Purisch

Theses and Dissertations--Geography

Epigenetic clocks are algorithms that use epigenetic biomarkers of aging to calculate an organism’s ‘biological age.’ Through discursive analysis of commercially available epigenetic clock marketing materials, scientific articles, and interviews with actors in the longevity biotechnology industry, this study examines the commercialization of epigenetic clocks in the form of consumer test-kits. The study argues that longevity biotechnology companies leverage the malleable and fluid qualities of epigenetic markers of biological age to extend the domains of profitable therapies. It shows that epigenetic clock companies market the dynamic qualities of epigenetic biological age measurements to redefine health around the project of optimizing …

Yaoi Lesbians: Media, Identity, And Desire, Olivia Marquardt

Theses and Dissertations--Linguistics

Media consumption reflects aspects of identity while also assisting in identity formation (Koller, 2008; Zemmels, 2012; Wu, 2006). This thesis examines the phenomenon of lesbians who engage with men-loving-men (MLM) media. The practice seems odd due to the contrast of lesbians desiring women for their social, platonic, romantic, and sexual relationships, but this thesis finds that repeated consumption of media that display men and their desires can index lesbianism. This practice helps lesbians construct their identities through three processes of indexicality: indexical disjuncture, indexical bleaching, and indexical denial (Barrett & Hall, 2024). Through a discourse analysis of five semi-constructed interviews …

“A True And Broad Sympathy:” Thomas Hughes, Medievalism, And Social Justice In The Nineteenth Century, Sarah E. Stapleton

Theses and Dissertations--History

Thomas Hughes, author of Tom Brown’s Schooldays (1857), used medievalism to help solve social problems of the Nineteenth Century. This contravenes the traditional view of medievalism, especially Anglo-Saxonism, since others have used it to justify the subjugation of people of lower social status, namely the poor in England, enslaved African Americans in the United States, and Indians under British rule.

While the use of medievalism is veiled in Hughes’ work, the subtext of Tom Brown’s Schooldays demonstrated Hughes’ Anglo-Saxonism. Tom Brown and the intertextual material surrounding his narrative exhibit the characteristics of Anglo-Saxonism through cultural primitivism, the concept of allodial, …

Targeting Prmt5 In Inflammation And Cancer: A Nexus Of Endotoxin Signaling, Antigen Presentation And Immune Evasion., Jimmy James Msemo

Theses and Dissertations--Medical Sciences

Protein Arginine Methyltransferase 5 (PRMT5) is a member of PRMT family that controls both intrinsic and extrinsic oncogenic processes [1]. Protein arginine methyltransferase 5 (PRMT5) is a type II methyltransferase that exerts widespread influence over gene expression, splicing, DNA repair, and immune regulation through symmetric dimethylation of arginine residues on histone and non-histone proteins [2]. The addition of methyl groups induces gene silencing through producing dictatorial histone marks like H2AR3ne2s, H3R8me2s and H4R3mes [3], which act as epigenetic repressive marks on gene promoters. In non-histone proteins, PRMT5 can methylate and regulate transcription factor proteins such as p53, E2F1, and p65, …

Investigating Regulation And The Regulome Of The Nucleic Acid Binding Protein Spovg Within Borrelia Burgdorferi, Timothy C. Saylor

Theses and Dissertations--Medical Sciences

Prior studies indicated Borrelia burgdorferi SpoVG protein was regulated, SpoVG binds specifically to a number of nucleic acids, and SpoVG binds to the non-variable section of the major virulence factor vlsE. Although a direct transcriptional activator or inhibitor was not elucidated, it is shown that SpoVG is regulated based on cell culture density independent of the growth rate of B. burgdorferi, indicating that SpoVG regulation is outside the scope of the alternative sigma factor RpoS. Using a dCas9 CRISPRi construct to target SpoVG resulted in protein expression trending towards upregulation of the BosR-RpoS regulon, and a marked decrease …

Cellular And Behavioral Consequences Of Psychedelic Drug Action In The Claustrum, Tanner L. Anderson

Theses and Dissertations--Neuroscience

Psychedelics are rapidly garnering research attention for their ability to successfully treat a variety of psychiatric diseases after one or a handful of treatments in recent clinical research. Among the conditions treated is substance use disorder (SUD), which afflicted 48.5 million (16.7%) Americans aged 12 and older in 2023 (NSDUH). As clinical trial results continue to show lasting positive effects of psychedelic psychotherapy, there remains an enormous gap in knowledge regarding how these drugs cause lasting changes in the brain to achieve these therapeutic effects. It has been suggested that psychedelics must induce rapid and long-lasting neuronal plasticity in order …

Leveraging Clinical Standards To Improve The Translational Relevance Of Therapies For Experimental Spinal Cord Injury, Aaron L. Silverstein

Theses and Dissertations--Neuroscience

Efforts to improve treatment of spinal cord injury (SCI) often use animals to develop effective therapeutics, intending to improve the survival and the quality of life of persons with SCI. Over half of all SCIs in the United States occur at the cervical level and often cause locomotor deficits and life-threatening breathing dysfunction. Thus, my dissertation is focused on this important patient population.

After beginning with a description of translational research and surveying SCI from a clinical standpoint, I turned to describe experimental models of cervical SCI and more detailed neuroimmunological findings relevant to my work. Then, I conducted a …

Understanding The Impact Of The Medicaid Expansion On Hospital Length Of Stay And Emergency Department Use In Kentucky, Cameron Bushling

Theses and Dissertations--Epidemiology and Biostatistics

Three related analyses were performed to try to understand the immediate effects of Medicaid expansion on hospital systems in Kentucky. Medicaid expansion led to a large, sudden increase in the number of individuals eligible for various health care needs. This sudden increase in demand for health services lead to initial hypotheses regarding hospitals’ ability to handle this demand. The first analysis examined hospital average length of stay (LOS) using a linear mixed-effects model. Length of stay was modeled longitudinally between 2013 and 2015 to determine if significant changes in the slope of LOS could be detected after expansion (2014). Separate …

Using Hyperactive Shp-2 Mutants, A Phosphatase, To Identify Interactions With The Rna Helicase Ddx3, Devin Montgomery

Theses and Dissertations--Pharmacy

Protein-protein interactions are critical in cellular signaling pathways. This project investigates binding interactions between SHP-2, a tyrosine phosphatase, and DDX3, an RNA helicase. There is a specific focus on the impact that gain-of-function SHP-2 mutations may impact binding to DDX3 via conformational change. Using immunoprecipitation protocols as well as western blotting we were able to provide insights into SHP-2 molecular mechanisms and subsequent binding to DDX3.

The Impact Of Maternal Environmental Exposures On The Immune Landscape Of The Maternal-Fetal Interface, Heather E. True

Theses and Dissertations--Pharmacy

Maternal environmental exposures and inflammation are known to impact fetal development in utero as well as increase the risk for chronic diseases later in life. Therefore, it is essential that we understand how the maternal environment alters placental structure and immune function.

Therefore, we isolated fetal (chorionic villous) tissues from the placentas of healthy pregnant participants (control) and those with inflammatory conditions, including SARS-COV-2 infection, pregravid obesity, and opioid use disorder. Isolated placental leukocytes were subjected to a series of assays to uncover the phenotypic, functional, and transcriptional (single-cell RNA-sequencing) landscape of placental immune cells. Additional placental tissues were subjected …

Simplified Molecular Diagnostics Methods For Environmental Testing Of Pathogens And Other Applications, Mohammad Dehghan Banadaki

Theses and Dissertations--Mechanical and Aerospace Engineering

Environmental Surveillance (ES) has emerged as a powerful tool to monitor the emergence and persistence of a pathogen within a facility or community using wastewater/water analysis. Over the past four years, implementation of ES has experienced an explosive acceleration, driven by efforts to monitor the spread of SARS-CoV-2, which has made ES a routinely used public health tool in many locations. We can now use ES to measure the emergence/presence/abundance of different viruses (e.g., SARS-CoV-2, influenza, mpox), bacteria (e.g., E. coli, V. cholerae, antibiotic-resistant strains), fungi (e.g., C. auris), and other biological markers indicative of infectious diseases. However, its broad …

Numerical Investigation Of Wind Disturbance Around A Multirotor Uav In Crosswind And Vertical Flight, Garrison Page

Theses and Dissertations--Mechanical and Aerospace Engineering

Uncrewed Aerial Vehicles (UAVs) are being increasingly utilized in a wide range of research applications, especially in meteorology. This is due to such factors as their ease of use, maneuverability compared to other forms of data collection, and for rotor- craft, the ability to hover. However, the rotorwash generated by UAVs can introduce significant disturbances, potentially affecting measurement accuracy from sensors. While several studies have investigated the rotorwash of individual UAVs utilizing Particle Image Velocimetry (PIV) and Computational Fluid Dynamics (CFD), there is limited exploration into how different rotor configurations and external wind conditions influence regions of turbulent flow around …

Machining-Induced Residual Stress Modeling Using Physics-Informed Neural Networks, Md Mehedi Hasan

Theses and Dissertations--Mechanical and Aerospace Engineering

Process-induced residual stress (RS) plays a critical role in determining the structural integrity, fatigue life, corrosion resistance, and dimensional stability of manufactured components. Tensile residual stress can be harmful to the machined workpiece by lowering fatigue strength, while compressive residual stress helps to prevent such issues. Therefore, accurate prediction and control of machining-induced residual stresses are essential for optimizing manufacturing processes and enhancing component performance. However, existing modeling approaches—empirical, analytical, and numerical—often have limited predictive accuracy, demand extensive calibration, or involve high computational costs. Traditional finite element analysis (FEA) provides modeling insights but is constrained by its high computational cost …

Fluid Pathways For Enhanced Mass Transfer In The Micro Regime: Applications In Novel Pumpless Redox Flow Cell And Zinc Finger Protein-Based Dna Detection, Shaikh Al Mahmud Bhuiyan

Theses and Dissertations--Mechanical and Aerospace Engineering

Precise fluid transport through microscale geometries can help optimize electrochemical and biochemical processes by maximizing the mass transfer rate of reactants. By introducing intricate structural features inside microchannels, it is possible to manipulate the fluid motion and direct the flow towards any surface of interest. In this study, we have demonstrated that rationally designed fluid pathways can improve the performance of electrochemical and biochemical systems.

The emergence of additive manufacturing has enabled rapid fabrication of robust structures with intricate features to guide fluid motion. However, their application in nonaqueous electrochemical systems has been limited due to their interaction with organic …

Direct Numerical Simulation Of Pulsating Flow In Curved Pipes: Insights Into Aortic Dissection In Humans, Gokul Anugrah Gopakumar

Theses and Dissertations--Mechanical and Aerospace Engineering

This dissertation investigates the hemodynamics of pulsating blood flow in curved vessels through direct numerical simulations (DNS), with the overarching aim of uncovering fluid dynamic mechanisms linked to the onset and progression of aortic dissection in humans. The work is structured in two parts: (i) fundamental studies in idealized geometries and (ii) preliminary investigations in patient-specific anatomies. In the first part, curved pipe models representing the aortic arch are used to isolate the effects of pulsation frequency, amplitude ratio, and curvature ratio on transitional and turbulent flow dynamics. The simulations reveal that curvature-driven centrifugal forces shift the peak velocity toward …

Impact Of Hyperthermia And Mitomycin C On Heat Shock Proteins, Mismatch Repair Proteins, And Cytotoxicity In Colon Cancer, Anna M. Reagan

Theses and Dissertations--Clinical and Translational Science

Cytoreductive surgery and heated intraperitoneal chemotherapy (HIPEC) is a treatment used for primary peritoneal malignancy or metastases, including colon cancer. We aimed to explore the effects of hyperthermia and/or mitomycin C (MMC) on mismatch repair (MMR) proteins and heat shock proteins (hsps) and the potential for post-HIPEC treatment with pembrolizumab treatment. Colon cancer cells were treated with hyperthermia and/or MMC for 90 minutes. Subcellular fractionation and immunofluorescent imaging were performed to identify protein abundance and localization of MSH2, MLH1, hsp70, and hsp27. Proteomics analysis was performed for HT29 cells. HT29 cells and patient-derived organoids (PDOs) underwent simulated HIPEC and treatment …

M1 Macrophage-Engineered Vesicles Have Anti-Cancer Activity In Ovarian Cancer, Connie Du Cao

Theses and Dissertations--Clinical and Translational Science

Ovarian cancer typically presents at an advanced stage, has a poor prognosis, and is a leading cause of cancer-related deaths in women. Extracellular vesicles (EVs) are cell-derived membrane-bound nanoparticles that function in specific cell-to-cell communication and are under development as novel drug delivery vehicles and modulators of the tumor microenvironment. Artificial cell-derived vesicles (ACDVs) derived from M1 macrophages are able to repolarize M2 macrophages to the M1 phenotype and target tumor cells in in vitro studies.

In this study, we generated engineered EVs (EEVs) by membrane disruption of M1 macrophages (MEVs) in the presence and absence of cisplatin to generate …