Digital Commons Network^™

Development Of Zafirlukast Analogues For Improved Antithrombotic Activity Through Thiol Isomerase Inhibition, Justine A. Keovilay, Kaitlind C. Howard, Kirk A. Taylor, Sabeeya Khan, Sienna E. Wurl, Melanie K. Szahaj, Tanya Sage, Nishad Thamban Chandrika, Caixia Hou, Oleg V. Tsodikov, Jonathan M. Gibbins, Sylvie Garneau-Tsodikova, Daniel R. Kennedy

Pharmaceutical Sciences Faculty Publications

Background:   Thiol isomerases play essential and nonredundant roles in platelet activation, aggregation, and thrombus formation. Thiol isomerase inhibitors have the potential to overcome the 2 major drawbacks of current antithrombotic therapies, as they target both arterial and venous thrombosis without enhancing bleeding risks. Recently, a Food and Drug Administration–approved drug, zafirlukast (ZAF), was shown to be a promising pan-thiol isomerase inhibitor. The objective of this study is to develop analogues of ZAF with optimized thiol isomerase inhibition and antithrombotic activity.   Methods:   Thirty-five ZAF analogues were tested in an insulin turbidometric assay for thiol isomerase inhibition. Analogues were tested for platelet …

Functional And Structural Studies On The Esperamicin Thioesterase And Progress Toward Understanding Enediyne Core Biosynthesis, Erome D. Hankore, Mitchell D. Miller, Abigael J. Kosgei, Weijun Xu, Kemin Tan, Michael Endres, Minakshi Bhardwaj, Grazyna Joachimiak, Andrzej Joachimiak, George N. Phillips Jr., Jon S. Thorson, Steven G. Van Lanen

Pharmaceutical Sciences Faculty Publications

Enediynes are among the most potent antitumor and antibacterial natural products. Studies on their biosynthetic pathways have identified a shared, linear polyene precursor generated from an iterative type I polyketide synthase (PKSE) as the source of the enediyne warhead. A key step is the release of this polyene from the PKSE by a discrete thioesterase (TE). Here, we used X-ray crystallography, site-directed mutagenesis, and heterologous coexpression of PKSEs and TEs to elucidate how enediyne TEs mediate the production of the polyene. We solved the structure of wild-type EspE7 from esperamicin producer Actinomodura verrucosospora. The substrate binding pocket was also …

Determining The Pubertal Age Of Female Subadults Within The New Mexico Decedent Image Database, Jessica Destiny Parenti

Electronic Theses and Dissertations

Adolescence marks a critical period of social and biological transition, characterized by the onset of puberty—a pivotal milestone indicating sexual maturity. Shapland and Lewis (2013, 2014) proposed a method for tracking pubertal stages within the skeleton, encompassing observations of various skeletal developments such as the mandibular canine, hamate bone, distal radius, phalanges, and cervical vertebrae 3-5. This study analyzes the pubertal stages in 26 subadult females, aged eight to 20, from the New Mexico Decedent Image Database. By integrating the extensive background information provided by the New Mexico Decedent Image Database, this study explores the factors influencing the onset of …

Full Issue

BYU Studies

No abstract provided.

Front Matter

BYU Studies

No abstract provided.

Enhancing Topical Delivery Of N-Acetylcysteine And Collagen Via A Novel Electrospun Collagen/Pmma Nanofibrous Mats As Facial Mask Development: Nanofibers Optimization And In Vitro Experiments, Abdullah Elbadry, Elbadawy Kamoun, Mahmoud Sabahy, Mohamed Helmy, Mahmoud Khaled, Shahira Elmosalamy, Noura Eissa

Nanotechnology Research Centre

No abstract provided.

Academic Proficiency In Ugandan Children With Sickle Cell Anemia: A Cross-Sectional Study, Robert Opoka, Shubaya Kasule Naggayi, Paul Bangirana, Simple Ouma, Betty Nyangoma, Annet Birabwa, Grace Nambatya, Maxencia Kabatabaazi, Ann Jacqueline Nakitende, Dennis Kalibbala

Paediatrics and Child Health, East Africa

Introduction: Academic proficiency is crucial for advancing learning goals, school advancement, and future economic security. Children with sickle cell anemia (SCA) in sub-Saharan Africa (SSA) may be at risk of disease-associated learning difficulties. Limited data exist on this topic among school-age children in the region. We aimed to assess academic proficiency in a sample of children with SCA in Uganda compared with unaffected controls.

Methods: This cross-sectional study was conducted in Kampala, Uganda. Participants were school-going children with SCA, aged 6–12 years, attending Mulago Hospital SCA Clinic, and age-matched sibling controls without SCA. Academic proficiency was assessed by the Wide …

Identification Of Host-Pathogen Protein Interactions: Yeast Two-Hybrid Analysis Of Sars-Cov-2 Nsp10 And Human Cellular Proteins, Antonios Argyriou

Selected Full-Text Master Theses 2021-

The ongoing COVID-19 pandemic, caused by SARS-CoV-2, has resulted in over 774 million cases and 7 million deaths worldwide, establishing it as one of the most devastating pandemics in recorded history. While current therapeutic approaches primarily target viral entry mechanisms and specific enzymes, the limited understanding of non-structural protein functions hinders the development of comprehensive antiviral strategies. This research focuses on Nsp10 (non-structural protein 10), a highly conserved 139-amino acid protein essential for viral mRNA capping and replication. Nsp10 serves as a critical cofactor in the formation of methyltransferase complexes with Nsp14 and Nsp16, playing a vital role in viral …

Coordinating The Energetic Strategy Of Glia And Neurons For Memory, Hannah Shoenhard, Amita Sehgal

Biology Faculty Research and Scholarship

Memory consolidation requires rapid energy supply to neurons. In a recent study, Francés et al revealed the signal by which a neuron commands glia to limit fatty acid synthesis in favor of metabolite export during memory formation in Drosophila melanogaster. This mechanism coordinates just-in-time glial energy delivery in response to dynamic neuronal needs.

Multiscale Modeling Of Infectious Diseases: Identifiability Framework For Parameter Estimation And Model Validation, Ambegoda Liyanage

Electronic Theses and Dissertations

Understanding and controlling the spread of infectious diseases requires integrative mathematical models that connect biological processes across multiple scales, from individual-level transmission dynamics to population-level epidemic behavior. In this work, we develop and analyze multiscale mathematical models to investigate the transmission of emerging and re-emerging infectious diseases, with a strong focus on structural and practical identifiability to ensure model reliability.

We begin by investigating the transmission dynamics of the Usutu virus (USUV), an emerging mosquito-borne virus of growing concern in Europe and Africa. Understanding the epidemiology of such pathogens requires a systems-level approach that captures biological processes across multiple scales, …

Semi Analytical Solution Ofmhd And Heat Transfer Of Couple Stress Fluid Over A Stretching Sheet With Radiation In Porousmedium, Sara Abdelsalam, M. Khairy, W. Abbas, A.M. Megahed, M.S. Emam

Basic Science Engineering

This comprehensive research examines the dynamics of magnetohydrodynamic (MHD) flow and heat transfer within a couple stress fluid. The investigation specifically focuses on the fluid’s behavior over a vertical stretching sheet embedded within a porous medium, providing valuable insights into the complex interactions between fluid mechanics, thermal transport, and magnetic fields. This study accounts for the significant impact of heat generation and thermal radiation, crucial factors for enhancing heat transfer efficiency in various industrial and technological contexts. The research employs mathematical techniques to simplify complex partial differential equations (PDEs) governing fluid flow and heat transfer. Specifically, suitable similarity transformations are …

Numerical Simulation Via Homotopy Perturbation Approach Of A Dissipative Squeezed Carreau Fluid Flow Due To A Sensor Surface, Sara Abdelsalam, W. Abbas, A.M. Megahed, H. M.H. Sadek, M.S. Emam

Basic Science Engineering

This study rigorously examines the interplay between viscous dissipation, magnetic effects, and thermal radiation on the flow behavior of a non-Newtonian Carreau squeezed fluid passing by a sensor surface within a micro cantilever channel, aiming to deepen our understanding of heat transport processes in complex fluid dynamics scenarios. The primary objective is to elucidate how physical operational parameters influence both the velocity of fluid flow and its temperature distribution, utilizing a comprehensive numerical approach. Employing a combination of mathematical modeling techniques, including similarity transformation, this investigation transforms complex partial differential equations into more manageable ordinary ones, subsequently solving them using …

The Flowers Of Being And Time: Nature And Heidegger’S Abandonment Of His Early Project, Robert J. Dostal

Philosophy Faculty Research and Scholarship

No abstract provided.

Production & Perception Of /S/ And Pitch Of Transgender Speakers, Connor J. Ryan

Honors Theses and Capstones

Linguistic research on how transgender speakers use speech traits commonly associated with gender, such as f0 and /s/, is still limited in scope. Past research has focused primarily on singular gender groups within the transgender community, particularly transmasculine speakers, leaving gaps in how speech differs among transgender speakers. Additionally, there has been little research on how transgender identity influences perception of gender in speech. Here, I use data from reading passages by fifteen university-age transgender speakers in New England to analyze variation in /s/ and f0 among transmasculine, transfeminine, nonbinary, and multigender speakers. I then performed a perception study using …

Skeletons At Sea: Unpacking The Oceanographic And Taphonomic Variables That Impact The Remains Of Decedents At Sea, Ella Tartsa

Honors Theses and Capstones

No abstract provided.

Sensory Need Or Treatable Behavior? Self-Stimulatory Behavior In Autism Spectrum Disorder: A Systematized Review, Anne Grace, Kathryne Brewer

Honors Theses and Capstones

This study examined the current dialogue about Autism Spectrum Disorder (ASD) and

self-stimulatory behavior (stimming), based on a systematized literature review. Stimming is

often framed as a maladaptive or disruptive behavior that negatively impacts autistic individuals.

It is important to investigate whether these claims are supported by current research. The

following research question was used to guide this paper: How does current research on ASD

conceptualize stimming, as a form of sensory self-regulation or a treatable behavior?

The Usual Suspects: A Review Of Common Dysbiosis Of The Human Mouth, And The Associated Microbial Species, Sophie Katherine Shields

Honors Theses and Capstones

The mouth, often referred to as the oral cavity in scientific literature, is described as the second most diversely populated microbiome in the human body, only after the gut. The cavity’s temperature, abundant water, high nutrient levels, and diversity of sites are all critical factors that make the oral cavity a space booming with life. The mouth harbors over 700 species of bacteria, in addition to numerous fungi, viruses, and protozoa species. Together, when working in harmony, these species provide critical supports to the functions of the human body. When dysbiosis occurs, however, the overgrowth of a single species may …

Development Of A Clickable Probe For Imaging Hydrogen Peroxide In The Bilayer, Erin Elizabeth Mccarthy

Honors Theses and Capstones

No abstract provided.

Using Rna Sequencing To Examine Encephalic Gene Expression In Cactus Mice, Sarah A. Nicholls

Honors Theses and Capstones

Many of the exact mechanisms by which desert-adapted animals avoid dehydration-induced complications are unknown, though there are likely ties to the general mammalian dehydration response, a multisystem process spearheaded by the brain. Peromyscus eremicus, the cactus mouse, can survive its whole life without intaking water and is commonly used as a model to study desert adaptations. In this study, we have used bulk transcriptomics to characterize the differential gene expression across hydrated and dehydrated P. eremicus in five major regions of the brain: the cerebral cortex (referred to throughout much of this paper as the cortex), hippocampus, thalamus, midbrain, …

Molecular Simulation Studies Of Protein Interactions Associated With Aging, Charlotte Jenna Thomas

Honors Theses and Capstones

No abstract provided.

Investigating The Dna-Binding Role Of Tbp1 In Toxoplasma Gondii: Expression And Characterization Of A Recombinant Protein, Madison T. Jeckell

Honors Theses and Capstones

Toxoplasma gondii is an obligate intracellular parasite with a complex life cycle and an unusual transcriptional machinery compared to other eukaryotes. Unlike most organisms, Toxoplasma lacks multiple well-conserved promoter motifs and has multiple TATA-binding proteins (TBPs), including TBP1, whose function in DNA binding and transcription regulation is poorly understood. This study aimed to generate and express a recombinant TBP1 protein from Toxoplasma to enable future DNA-binding assays and motif identification. Using RT-PCR, the TgTBP1 gene was amplified and cloned into a pGEX vector to produce a GST-tagged protein. The recombinant plasmid was transformed into E. coli BL21 cells, and protein …

Reconceptualizing Madness: Charlotte Perkins Gilman's Autobiography And The Cultural Landscape Of Insanity, Gender, And The Illness Experience, Claire Paley

Honors Theses and Capstones

No abstract provided.

Targeting Stat3 In Tnbc Drug Resistance, Madison A. Henry

Honors Theses and Capstones

Triple negative breast cancer (TNBC) is one of the most aggressive types of breast cancer and lacks the three commonly targeted receptors in breast cancer treatments. As a result of a lack of targetable receptors, the standard of care remains to be harsh chemotherapy treatments that often are successful in early TNBC cases but often result in relapsed TNBC. In addition to high rates of recurrence, TNBC is known for its aggressive metastasis and chemoresistance. Within TNBC, the transcription factor Signal Transducer and Activator of Transcription 3 (STAT3) is constitutively active and increases proliferation, and metastasis, and plays a role …

Reasoning Through Change: Exploring Covariational Thinking In Student Exponential Graph Interpretation, Samantha Lynn Pearson

Honors Theses and Capstones

This study explores how life science students reason about exponential functions in the context of an introductory physics course. While exponential functions are essential in both physics and biology—appearing in scenarios like population growth and temperature cooling—students often encounter challenges in interpreting them. Using a resources framework, we examined the cognitive resources these students bring to exponential reasoning, focusing on what ideas and strategies they already possess. Our methodology involved conducting think-aloud interviews with nine students from PHYS 402. Interviews were coded using a framework informed by prior research on covariational reasoning and exponential understanding. Results show that students generally …

Waking The Witch: Exploring Gender, Magic, And Power Dynamics In Ancient Rome, Diana Gibson

Honors Theses and Capstones

This thesis explores the presence of witches and magic in the literature of the Roman Empire. The study ranges from the 1st century BCE to the 6th century CE, and relates the prevalence of female witches in the Roman literature to particularly turbulent social contexts, such as the fall of the Republic and the rise of Christianity. The piece considers the rhetorical nature of accusations of witchcraft, and how the concepts of magic and witchcraft themselves were defined and shaped by those in power to uphold imperial hegemony and structures of patriarchy. The project seeks to highlight a distinction between …

18s Metabarcode Analyses Of Eukaryotic Species In The Respiratory Microbiomes Of Wild Canids From New Hampshire, Collin Sinclair Blake

Honors Theses and Capstones

This study is of an exploratory nature and focuses on characterizing the eukaryotic microbiota present in the respiratory tissues of six wild canids and one domestic canine. The contents of this document largely pertain to dry lab analyses of 18S barcodes in bioinformatics programs – primarily QIIME2 running in the GitBash command line, service for which was hosted by the UNH Ron Bioinformatics training server. All procedures listed within the section below were performed by second parties at the UNH Hubbard Center for Genomics Studies (HCGS), the New Hampshire Veterinary Diagnostics Lab (NHVDL), and the UNH Microbial Ecology and Emerging …

Optimization Of Small-Scale Bioreactors For Increased Pdna Production And Yield, Laura M. Hills

Honors Theses and Capstones

No abstract provided.

What The Earth Remembers: Landscape And Materiality In The Work Of Sally Mann, Mark Bradford, And Tacita Dean, Elliot Krasnopoler

Bryn Mawr College Dissertations and Theses

This dissertation triangulates the complex relationships among materiality, place, and history in works of contemporary landscape. I analyze three case studies: Sally Mann’s photographs of the American South, Mark Bradford’s iconoclastic paintings after the Gettysburg Cyclorama, and Tacita Dean’s film of the Great Salt Lake. I contend that these contemporary works of art are capacious in their temporal and narrative reach, holding multifaceted histories of America, including those of environment, war, oppression, and extraction. A key part of my thesis is that by looking at how these works were made and what they are made of—their materials, places, and processes—we …

Eugenol Loaded Calcium Citrate Particle As A Ph Triggered Drug Release System And A Reducing Agent For Au (Iii), Sadia Afrin

Dissertations and Theses

The study demonstrated that eugenol affects the morphology of calcium citrate particles during their formation, as confirmed by SEM micrography and PXRD analysis. Coating of eugenol loaded calcium citrate particles (CaCit MPs) with casein improves their known pH-dependent drug release profile, as confirmed by UV absorption spectroscopy. The casein-coated eugenol-loaded CaCit MPs (CCaCit(eu) MPs) can effectively control and sustain drug release under different pH conditions, particularly at acidic pH values (4.8 and 5.5), which mimics the environment of tumor tissues. To further explore the biomedical applications of calcium citrate, a bulk nanostructured precursor to the known anti-cancer calcium citrate phosphate-based …

Periconceptual Nutrition And The Preimplantation Embryo: Approaches For Intervention And Generational Health, Abigail Klein

Dissertations and Theses

The typical American diet exceeds dietary fat recommendations, contributing to the rising rates of obesity. This negatively impacts fertility, pregnancy and fetal growth, and lifelong offspring health. During pregnancy, this also increases risk of co-morbidities like gestational diabetes. Despite this, lipids are not screened during pregnancy and limited research exists evaluating these co-exposures on offspring health. Our lab used a rat model of maternal high fat (HF) diet with late-gestation diabetes (DM) to demonstrate that mitochondrial dysfunction and oxidative stress incite lifelong cardiometabolic disease risk in offspring. To determine the timing of damage, we hypothesized that maternal overnutrition incites mitochondrial …