Digital Commons Network^™

Resistance Exercise And Mechanical Overload Upregulate Vimentin For Skeletal Muscle Remodeling, Joshua S. Godwin, J. Max Michel, Cleiton A. Libardi, Andreas N. Kavazis, Christopher S. Fry, Andrew D. Frugé, Mariah Mccashland, Ivan J. Vechetti, John J. Mccarthy, C. Brooks Mobley, Michael D. Roberts

Physiology Faculty Publications

We adopted a proteomic and follow-through approach to investigate how mechanical overload (MOV) potentially affects novel targets in skeletal muscle, and how a perturbation in this response could potentially affect the adaptive response. First, we determined that 10 wk of resistance training in 15 college-aged females increased sarcolemmal-associated protein content (+10.1%, P < 0.05). Sarcolemmal protein isolates were then queried using mass spectrometry-based proteomics, ∼10% (38/387) of proteins putatively associated with the sarcolemma or extracellular matrix (ECM) were upregulated (>1.5-fold, P < 0.05), and one target (intermediate filament vimentin; VIM) warranted further investigation due to its correlation to myofiber hypertrophy (r = 0.652, P = 0.009). VIM expression was then examined in 4-mo-old C57BL/6J mice following 10 and 20 days of plantaris MOV via synergist ablation. Relative to Sham (control) mice, VIM mRNA and protein content was significantly higher in MOV mice, and immunohistochemistry indicated that VIM predominantly resided in the ECM. MOV experiments were replicated in Pax7-DTA (satellite cell depleted) mice, which reduced VIM in the ECM by ∼74%. A third MOV experiment was performed in C57BL/6 mice intramuscularly injected with either AAV9-scrambled (control) or AAV9-VIM-shRNA. Although VIM-shRNA mice possessed lower VIM in the ECM (∼45%), plantaris masses in response to MOV were similar between groups. However, VIMshRNA mice possessed smaller and more centrally nucleated MyHCemb-positive fibers in response to MOV. In summary, skeletal muscle VIM appears to be enriched in the ECM following MOV, satellite cells may regulate its expression, and a disruption in expression during MOV leads to an excessive regenerative phenotype.

Investigating The Relationship Between Cellular Senescence And Pulmonary Fibrosis, Crystal Lee

Theses

This study investigates the complex relationship between cellular senescence, aging, and pulmonary fibrosis through a comprehensive analysis of bulk RNA-seq data from patients with idiopathic pulmonary fibrosis (IPF), chronic obstructive pulmonary disease (COPD), and scleroderma. Using differential expression (DE) and age-associated differential expression (AADE) analyses, we revealed a paradoxical pattern: senescence-related gene sets were significantly downregulated in disease comparisons but progressively upregulated with aging within these same diseases. Gene set enrichment analysis (GSEA) revealed that gene sets associated with DNA damage/telomere stress and oxidative stress were among the most enriched senescence inducers in fibrotic disease states, while cellular senescence and …

Methionine Matters: Comparative Dynamic Insights Into Viral Inhibition Of Host Rna Export Via Rae1-Nup98, Skye Bixler

Theses

Over time, viruses have evolved a myriad of mechanisms to gain an advantage in the virus-host battle, such as inhibiting host mRNA export through RNA Export Factor 1 (Rae1) and Nucleoporin 98 (Nup98). This strategy employed by the Vesicular Stomatitis Virus (VSV) M protein, Kaposi’s Sarcoma-Associated Herpesvirus (KSHV) ORF10, and SARS-CoV-1/2 ORF6 disrupts antiviral production. Despite their evolutionary distance, these viral proteins share a key methionine flanked by acidic residues that competitively bind to the ssRNA binding pocket of Rae1. The VSV mutant, R1, carries an M51R mutation that disrupts viral inhibition and restores host gene export— a pattern observed …

Forecasting Load At Residential, Industrial, And Commercial Stations For Dubai Electricity And Water Authority: A Machine Learning Approach To Managing Generation During Peak And Off-Peak Times Over The Coming Years, Mohammad Anoohi

Theses

In Dubai, with the rapid growth of energy demand, efficient demand side management is necessary to optimize the distribution of electricity, reduce peak loads, and improve grid stability. Traditional DSM strategies are based on historical data and reactive control mechanisms that cannot adapt to evolving consumption patterns. This research uses Machine Learning techniques to enhance DSM in residential, commercial, and industrial sectors by developing predictive models that forecast energy demand based on seasonal variations. The dataset used was from Dubai Electricity and Water Authority (DEWA), covering the consumption patterns across Summer, Winter, Transition from Winter to Summer, and Transition from …

Reducing Co2 Levels In A Classroom Through The Use Of Biofilters, Jenna Rosser

Theses

We spend 80% of our time indoors and are seeing an increase in indoor pollutants (Sharma et al., 2022). With the push to create more energy efficient buildings, the envelopes are becoming more airtight. As a result, indoor air quality (IAQ) has decreased due to the inability of indoor pollutants to escape from the building as easily through cracks and seams. (Irga et al., 2018; Satish et al., 2012). To date, there is a lot of existing research in regard to the role biofilters play in improving IAQ. Research ranges from having examined specific parts of the plants, soil conditions, …

Using Generative Ai For Tutoring Data Science, Yusra Khalid

Theses

A large increase in the use of Generative AI has been observed in the last few years. Data science is also a rapidly growing field with a high demand for skilled professionals. The goal of this thesis is to explore the potential of Generative AI, specifically ChatGPT, in facilitating data science education. The focus is on how ChatGPT can be used as a tutor to help solve practical exercises. The capabilities of Generative AI were explored along with the comparison of a few different models in terms of data science. Exploratory analysis was conducted to compare Generative AI models and …

Factors Influencing Community Assembly Of The Amphibian Microbiome In Rochester, Ny, Thomas R. Minahan

Theses

Amphibians are crucial in wetlands and, by extension, our planet. As predator and prey, amphibians serve as keystone species, vital for the continued equilibrium in their wetland ecosystems. However, the global amphibian population is currently threatened by the spread of a pathogenic fungus known as Batrachochytrium dendrobatidis (Bd). Over the past thirty years, Bd has spread worldwide, threatening hundreds of amphibian species. The environmental factors that influence microbial community assembly in amphibians are key to developing interventions that can protect amphibians from disease. This research examines the abiotic and biotic factors influencing microbial community composition in amphibians of Rochester, New …

Personalized Nutrition Recommendations For Arab Communities: Transforming Diet And Health Through Machine Learning, Khalfan Aldoobi

Theses

A lack of cultural understanding in traditional nutrition approaches prevents proper adherence to healthy dietary patterns among individuals. The Arab population faces unique challenges due to cultural dietary customs and lifestyle changes that have made obesity and diet illnesses major global public health challenges. The rapid shift to high-calorie but undernourished foods and less physical activity has resulted in alarming rate increases of obesity, cardiovascular diseases, and type 2 diabetes. The lack of cultural understanding in traditional nutrition approaches prevents proper adherence to healthy dietary patterns among individuals. This study focuses to fills the existing gap by applying Artificial Intelligence …

Steering Through Change: How Aging Eyes And Stroke-Induced Vision Loss Affect Driver Control, Arianna P. Giguere

Theses

Imaging system engineers could greatly benefit from understanding how biological systems have evolved to solve difficult imaging problems. In this thesis, we study how the human visual system has evolved to address the complex task of visually-guided steering, particularly as a person ages and experiences a prevalent type of vision loss from stroke called cortical blindness (CB) that results in the loss of one quarter to one half of the visual field. In a series of three studies, we test the hypothesis that the effects of CB on vision and steering extend beyond those that accompany healthy aging, and we …

Here Be Dragons: The Barriers Of Korean American Female Leaders In Secondary Schools, Joyce A. Kim

Dissertations

In K-12 public schools, there is a shortage of Korean American women in school leadership roles at the secondary level. This phenomenological study explores the contributing factors to this lack of representation, how institutions can recruit and retain Korean American female leaders, and how school systems can better support them. This study examines the leadership experiences of eight Korean American women. It utilizes the socio-political contexts of Korean patriarchy, the Model Minority Myth, and mentorship to help frame the problem of existing barriers. This study explores the following questions: What factors hinder Korean American women in school leadership? What structural …

Involvement Of Long Non-Coding Rna (Lncrna) Malat1 In Shear Stress Regulated Adipocyte Differentiation, Justin Caron, Marjan Ghanbariabdolmaleki, Madison Marino, Chong Qiu, Bo Wang, Michael Mak, Shue Wang

Biomedical Engineering Faculty Research and Publications

Adipocyte differentiation plays an important role in bone remodeling due to secretory factors that can directly modulate osteoblast and osteoclast, thus affecting overall bone mass and skeletal integrity. Excessive adipocyte differentiation within the bone marrow microenvironment can lead to decreased bone mass, eventually causing osteoporosis. The mechanical microenvironment of bone marrow, including fluid shear, maintains the balance of adipocyte and osteoblast differentiation during bone remodeling. However, how mechanical cues interact with long noncoding RNA (lncRNA) and regulate adipocyte differentiation remains unexplored. In this study, we investigated the mechanosensitive role of lncRNA MALAT1 during mesenchymal stem cells (MSCs) adipocyte differentiation. By …

Increasing Adolescents' Awareness Of The Impact Of Sleep And The Importance Of Healthy Sleep Habits, Vanessa Jennifer Diaz Salazar

Capstone Projects and Master's Theses

Middle school students often do not get the recommended amount of sleep each night and are unaware of the importance of sleep, negatively affecting their development. Poor sleep can affect their emotional well-being, academic performance, and physical health. When middle school students learn about better sleep hygiene, they can build better habits. To address this problem, I created a two-day lesson for eighth graders at the Dual Language Academy of the Monterey Peninsula in Seaside, California.

Fostering Scientific Learning With An Integrated Arts Curriculum For 5th And 6th Graders., George Donovan Martisius

Capstone Projects and Master's Theses

The traditional scientific curriculum presented in schools limits students’ ability to make creative connections with their work and develop critical thinking skills. This often leads to students not fully engaging in their learning and risks their ability to engage with science in a holistic way. Integrated curriculum combines different domains of learning that emphasize creative expression such as art with those that focus on abstract concepts and natural phenomena such as scientific learning. An Integrated science and art curriculum fosters deeper engagements and personal connection to their learning. Given the potential integrated curriculum has to foster scientific learning, I created …

Ai Model For Predicting Asthma Prognosis In Children, Elham Sagheb, Chung-Il Wi, Katherine S King, Bhavani Singh Agnikula Kshatriya, Euijung Ryu, Hongfang Liu, Miguel A Park, Hee Yun Seol, Shauna M Overgaard, Deepak K Sharma, Young J Juhn, Sunghwan Sohn

Faculty, Staff and Student Publications

BACKGROUND: Childhood asthma often continues into adulthood, but some children experience remission. Utilizing electronic health records (EHRs) to predict asthma prognosis can aid health care providers and patients in developing effective prioritized care plans.

OBJECTIVE: We aimed to develop artificial intelligence (AI) models using various clinical variables extracted from EHRs to predict childhood asthma prognosis (remission vs no remission) in different age groups.

METHODS: We developed AI models utilizing patients' EHRs during the first 6, 9, or 12 years of their lives to predict their asthma prognosis status at ages 6 to 9, 9 to 12, or 12 to 15 …

Association Of Delayed Asthma Diagnosis With Asthma Exacerbations In Children, Chung-Il Wi, Euijung Ryu, Katherine S King, Jung Hyun Kwon, Joshua T Bublitz, Miguel Park, Sergio E Chiarella, Jason D Greenwood, Thanai Pongdee, Lynnea Myers, Björn Nordlund, Sunghwan Sohn, Elham Sagheb, Bhavani Singh Agnikula Kshatriya, Dave Watson, Hongfang Liu, Beverley J Sheares, Carla M Davis, Wade Schulz, Young J Juhn

Faculty, Staff and Student Publications

Background: There is a significant delay between symptom onset and diagnosis of childhood asthma, but the impact of this delay on asthma outcomes has not been well understood.

Objectives: We sought to study the association of delayed diagnosis of asthma with asthma exacerbations (AEs) in children.

Methods: Using the Mayo Clinic birth cohort, we identified children with a diagnosis of asthma from electronic health records. We defined onset date as the date when subjects first met predetermined asthma criteria ascertained by an electronic health records-based natural language processing algorithm. Delay in diagnosis (DD) was defined as first diagnosis >30 days …

Course Insight Portfolio: Math 1060 Calculus Of One Variable I, Julianne Barnhart, Rayna Elizabeth Maleki, Antsa Rakotondrafara

Publications

We presented this Course Insight Portfolio project as a final assignment for the class Teaching Undergraduate Math MATH 9700 in Summer 2025. It covers the Clemson class MATH 1060 Calculus of One Variable I and is a fruit of the collaborative work of Julianne Barnhart, Rayna Maleki, and Antsa Rakotondrafara.

Libraries Facilitating Knowledge Creation Through Podcasting: Exploring Podcasts As A Form Of Scholarship, Annette Bochenek, Sarah Huber, Renusree Varma Mudduluru

Libraries Faculty and Staff Scholarship and Research

Students are increasingly expected to conduct research and scholarship. This paper explores podcast creation as a medium for students to deliver new knowledge and the elements of teaching and learning that support this outcome. Through focus groups, students at a large Midwestern university expressed the importance of contributing to the information landscape. The prospect of their podcasts contributing knowledge to academic communities held more interest to them than creating a podcast for an assignment. This paper addresses how librarians and libraries can support the information literacy standards and technical aspects of students creating new knowledge through a podcast assignment.

A Taste Of Rhetoric: Moves & Practices In The Decolonization & Restoration Of Historic Foodways, Abby A. Laforest

All NMU Master's Theses

Language and rhetoric are foundational influences on cultural practices, including culinary ones. The subdiscipline of food rhetoric studies communication about the processes of eating, cooking, transporting, and trading food, especially within the cultures of historically marginalized peoples. The past decades have seen a profusion of academic food research, but the role of language in understanding food, especially in a multicultural, globalized world, remains understudied. This paper examines how three rhetorical theories foundational to identity formation and layered understandings of meaning – (a) Dr. Karen Barad’s (they/them) theory of posthumanist performativity, (b) Dr. Maurice Charland’s theory of constitutive rhetoric, and (c) …

Effects Of Plant Growth-Promoting Microorganisms On Microbial Community Dynamics And Plant Growth Responses Of Aeroponically Cultivated Spinach, Veronica Snow

All NMU Master's Theses

Regional food insecurity caused by the inability to use conventional farming techniques year-round in cold climates increases the need for access to quality, locally sourced, and affordable foods year-round. Shifting from a soil-based system to aeroponics can increase yields, as aeroponic systems are not weather-reliant and can operate year-round. Aeroponics, a subgroup of hydroponics, induces growth by misting nutrient-rich solutions onto the plant roots. These systems use space and water more efficiently and rely less on fertilizers and pesticides but are vulnerable to errors in nutrient application, power loss, and rapid pathogen spread. Adding Vermicompost (VCT) and plant growth-promoting microorganisms …

Motherhood Within The Patriarchal Family: An Analysis Of “Frankenstein” And “The Scarlet Letter”, Yasamin Osqueezadeh

Undergraduate Honors Capstone Projects

Being a pivotal period in Western feminist history, the literary community in the 18^th century introduced pioneering feminists, the most famous of which is Mary Wollstonecraft, who called for a feminist revolution with A Vindication of the Rights of Women. Following the publication of A Vindication of the Rights of Women and other subsequent feminist papers, are Mary Shelley’s 1818 Frankenstein and Nathaniel Hawthorne’s 1850 The Scarlet Letter, two renowned pieces of Gothic literature. Although the two novels may seem disconnected, both Frankenstein and The Scarlet Letter engage in feminist arguments and critiques of the patriarchy in …

Minimizing Secondary Spinal Cord Injuries With Hypertonic Saline., Francisco A. Cortez-Thomas

Master of Engineering Theses

Spinal cord injury (SCI) disrupts white matter, causing periaxonal swelling, axonal degeneration, and impaired function. To examine vascular edema’s role, we developed a 3Dprinted dual-compartment imaging chamber to mimic and manipulate interstitial and vascular fluid dynamics in real time. We hypothesized that hypertonic saline (HTS) in the "vascular" chamber would osmotically shift fluid from the periaxonal space and preserve myelinated fibers after SCI. Using two-photon excitation microscopy, we imaged myelin and axons ex vivo in Thy1^YFP+ transgenic mice following C5 contusive SCI and assessed three clinically relevant HTS concentrations versus normal saline for their effectiveness in mitigating periaxonal swelling …

Kinetic Growth Rate Analysis Of Coelastrella Sp. In Aquaculture Wastewater., Rob Skarvan

Master of Engineering Theses

The demand for sustainable food and energy production continues to rapidly rise. Utilizing aquaculture wastewater as microalgae growth medium is a potential opportunity for nutrient recycling and industrial symbiosis, but more research is required to understand how best to design and scale-up a dual-purpose facility. This study investigates the growth of Coelastrella sp. in aquaculture wastewater compared to industry-standard growth medium BG-11 and modified tap water as controls. Though cell size and pigment density varied throughout media, aquaculture wastewater performed very comparably to BG-11 as a microalgae culture broth.

The Monod equation can model Coelastrella sp. growth based …

Investigation Of Immune/Performance Trade-Offs In Neonate Children's Pythons (Antaresia Childreni), Ellie Hartman

Undergraduate Honors Capstone Projects

Energetic costs and constraints are key considerations when analyzing an organism’s life history and may require trade-offs to be made between different life history traits. One of these trade-offs occurs between investment in immunity and performance. The purpose of this study is to discover if this trade-off influences behavior in neonate Children’s pythons (Antaresia childreni). We hypothesize that snakes undergoing an immune response will have reduced behaviors promoting survival. To test this hypothesis, neonate Children’s pythons were randomly assigned to receive an immune challenge of keyhole limpet hemocyanin (KLH) or a vehicle injection as a control. Two days …

Adaptation Of Influenza A Virus Strains A/Anhui/1/2013 (H7n9) And A/Shanghai/1/2013 (H7n9) For Development Of Effective Mouse Models, Spencer T. Mullen

Undergraduate Honors Capstone Projects

The emergence of avian Influenza A (H7N9) virus strains in humans poses a significant threat to human health globally due to their pandemic potential, limited human immunity, and lack of virus-specific interventions. This capstone aims to address some of the limitations in antiviral research regarding Influenza A H7N9 virus by adapting two low-pathogenic virus strains A/Anhui/1/2013 (H7N9) and A/Shanghai/1/2013 (H7N9) to BALB/c mice through sequential passaging through lung tissue. Following 12 serial passages, increased virulence was observed as evidenced by increased virus replication, increased weight loss, and increased mortality in infected mice. Titration of the initial non-adapted virus isolates were …

Survey Of Insect Pests On Cut Flowers In Utah, Elliot Roberts

Undergraduate Honors Capstone Projects

Cut flower production is a relatively new industry in the state of Utah, gaining momentum by 2020. However, little research concerning management of the crops and their pests has been conducted. One issue producers face are insects that harm the plants in ways such as feeding damage and transmission of diseases. Currently, it is unknown which insects target different species of cut flowers in Utah. Morphological identification of genera and species is often difficult or impossible because of the small size of many of the insects and minute differences between species. This project uses PCR and DNA sequencing to identify …

Creating A Stat2 Knock-Out Rat With Crispr/Cas9 Using I-Gonad, Isabella Dejesus

Undergraduate Honors Capstone Projects

Through the use of genetically modified animals, a more holistic understanding of physiologic responses to various diseases and pathologies can be obtained^7,19. The STAT2 gene encodes a protein called Signal Transducer and Activator of Transcription 2 and is an excellent target for genetic manipulation due to its essential role in type I and III interferon signaling pathways¹¹. A member of the STAT family of transcription factors, this protein is directly involved in mediating cellular responses to various cytokines and growth factors, particularly those related to the innate immune response¹¹. While the STAT2 signaling pathway …

Impact Of Altering Gut Microbiota With Multinutrient Supplementation On Neuropsychiatric Symptoms In Attention Deficit/Hyperactivity Disorder, Benjamin Hinkle

Physician Assistant Scholarly Project Papers

Attention-Deficit/Hyperactivity Disorder (ADHD) is a prevalent neuropsychiatric condition affecting children, often characterized by inattention, hyperactivity, and impulsivity. Recent evidence suggests a potential link between gut microbiota composition and neuropsychiatric disorders, including ADHD, through the gut-brain axis (GBA). A literature review of studies published between 2017 and 2024 explores the efficacy of multinutrient interventions on influencing gut microbiota to treat the core symptoms of ADHD in children ages 6-12 years old. Findings indicate that multinutrient supplementation may modulate gut microbiota and subsequently impact neuropsychiatric symptoms, offering a promising adjunctive treatment for ADHD. However, variability in study methodologies and small sample sizes …

Efficacy And Safety Of P2y12 Inhibitor Monotherapy Versus Dual Antiplatelet Therapy In Patients Who Have Received Drug Eluting Stents Post-Acute Coronary Syndrome, Jordan Saxerud

Physician Assistant Scholarly Project Papers

According to the American Heart Association, there are approximately 1.4 million people who are hospitalized and 700,000 deaths attributed to acute coronary syndrome on a yearly basis in the United States. It is standard therapy for patients to receive a drug eluting stent and dual antiplatelet therapy to prevent occlusion of the stent and other coronary arteries. Dual antiplatelet therapy typically consists of aspirin and a P2Y12 inhibitor. Dual antiplatelet leads to an increased risk of bleeding. This literature review aims to investigate if earlier initiation of P2Y12 inhibitor monotherapy decreases the risk of bleeding while maintaining adequate protection against …

Pharmacological Treatment Options For Nonalcoholic Steatohepatitis: Resmetirom Vs Glp- 1 Receptor Agonists, Debbie Zabel

Physician Assistant Scholarly Project Papers

The incidence of nonalcoholic steatohepatitis (NASH) is continually rising and has now emerged as one of the leading causes of cirrhosis requiring liver transplantation (Maurice et al., 2018). Until recently, the management of this disease has been limited to lifestyle modification and treatment of comorbid risk factors. In March 2024, the FDA approved resmetirom, the first and only medication for the treatment of this condition. The purpose of this literature review is to investigate the efficacy of GLP-1 and dual GLP/GIP or GLP/GCGR receptor agonist medications for the reduction of liver fat content in comparison to resmetirom. A literature review …

Comparing The Efficacy And Safety Of Glp-1 Receptor Agonists Versus Gastric Bypass Surgery In The Treatment Of Obesity And Type 2 Diabetes, Abir Oliver

Physician Assistant Scholarly Project Papers

Obesity and type 2 diabetes are interconnected global health challenges, contributing significantly to morbidity, mortality, and healthcare costs. This literature review compares the efficacy, safety, and applicability of glucagon-like peptide-1 (GLP-1) receptor agonists and bariatric surgery as treatment options. A literature search with defined inclusion and exclusion criteria was conducted using PubMed and EBSCOhost from May to July 2024, resulting in 14 randomized controlled trials for evaluation. This literature review supports the efficacy of both GLP-1 receptor agonists, particularly semaglutide, and bariatric surgery as effective interventions for weight loss and glycemic control in patients with obesity and type 2 diabetes. …