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Exploring Mobility: Phylogenetic And Structural Analysis Of The Intein In The Helicase Of Mycobacteriophage Alice, Sandra Xu

Honors Scholar Theses

This paper investigates the intein insertion in the DNA helicase gene Alice_175 of the Mycobacterium phage Alice, exploring its evolutionary significance and potential propagation mechanisms. Using phylogenetic analysis, there is evidence for gene transfer as a driving force in intein dissemination in phage and bacterial genomes. Structural predictions of the Alice_175 intein that were generated through AlphaFold2 as well as a phylogeny generated using SeaView infer a timeline of the intein's invasion and reveal key features that may influence protein functionality and host adaptation. Additionally, protein database searches suggest that intein mobility in this gene may not rely on prophages, …

Investigating The Ability Of The Norepinephrine-Dopamine Reuptake Inhibitor Nomifensine To Reverse The Effort-Related Effects Of Tetrabenazine In Female Rats, Vivien Daytree

Honors Scholar Theses

Anergia, lassitude, and psychomotor retardation are core symptoms of motivational dysfunction that often accompany Major Depressive Disorder (MDD). While selective serotonin reuptake inhibitors effectively alleviate mood and anxiety symptoms, they remain relatively ineffective at alleviating motivational deficits. Compounds that elevate extracellular dopamine (DA) have shown promise in addressing these symptoms. Bupropion, a norepinephrine-dopamine reuptake inhibitor (NDRI), increases DA and is effective at attenuating motivational symptoms in patients. Another NDRI, nomifensine, has demonstrated potential antidepressant properties in preclinical research. Effort-based choice tasks, such as the FR5/chow choice task, serve as animal models for studying motivational dysfunction. Rats choose between exerting effort …

Investigating The Factors Influencing The Rejection Of Cognitive Performance Tests In The Banking Sector Of Pakistan., Faraz Ali Memon, Uzair Inayat Memon

Business Review

This study explored the barriers hindering the adoption of cognitive performance tests in HR practices within Pakistan’s banking sector and developed practical recommendations for enhancing their implementation. A qualitative approach was adopted, and 16 semi-structured interviews were conducted with HR professionals from major banks in large cities. It aims to explore potential barriers to using and incorporating cognitive ability tests in selection practices. The data was later interpreted, and the findings highlighted five key themes. The results revealed factors influencing the rejection of cognitive performance tests in Pakistan's banking sector. It includes cultural factors such as senior management resistance and …

Effects Of Spatial And Temporal Variability On Bacterial Diversity And Community Composition In Roof-Harvested Rainwater Across Central Virginia, Jade M. Riddle

Longwood Senior Theses

Increasing global water scarcity has sparked interest in more sustainable water sources such as rainwater harvesting (RWH). Because these systems utilize roof-runoff for collection, they can easily be contaminated with environmental bacteria from animal feces and leaf litter, potentially degrading water quality. Thus, questions remain about the safety of harvested rainwater for human use due to the limited understanding of the microbial communities contained within it. To date, there is little published research on the microbiomes of rainwater harvesting systems (RWHS) within the continental United States. This study aimed to characterize the bacterial diversity and community composition of RWHS across …

Blockade Of Chpd1 T Cell Derived Cytokines Decreases Inflammatory Molecules Related To Cytokine Release Syndrome In Acute Lymphocytic Leukemia, Grace Cohen

Longwood Senior Theses

Acute lymphocytic leukemia (ALL) is a common pediatric cancer that is characterized by its high incidence of relapsed and refractory (R/R) cases. This brings about a need for new treatments that are safe and effective. One type of novel cancer immunotherapy uses chimeric antigen receptor (CAR) expressing T cells. CARs target tumor-associated antigens and enhance T cell responses. CAR T cell therapy has been successful in treating ALL, however there are associated toxicities such as cytokine release syndrome (CRS). One potential way to reduce CRS is to inhibit cytokine secretion and to alter the costimulatory domain in the CAR T …

Mrn-Ctip, Exo1, And Dna2-Wrn/Blm Act Bidirectionally To Process Dna Gaps In Parpi-Treated Cells Without Strand Cleavage, Isabelle M Seppa, Mithila Tennakoon, Jessica Jackson, Celia D Rouault, Sumedha Agashe, Vladislav O Sviderskiy, Mangsi Limbu, Erica Lantelme, Alice Meroni, Priyanka Verma, Alessandro Vindigni, Et Al.

2020-Current year OA Pubs

Single-stranded DNA (ssDNA) gaps impact genome stability and PARP inhibitor (PARPi) sensitivity, especially in BRCA1/2-deficient tumors. Using single-molecule DNA fiber analysis, electron microscopy, and biochemical methods, we found that MRN, CtIP, EXO1, and DNA2-WRN/BLM resect ssDNA gaps through a mechanism different from their actions at DNA ends. MRN resects ssDNA gaps in the 3'-to-5' direction using its pCtIP-stimulated exonuclease activity. Unlike at DNA ends, MRN does not use its endonucleolytic activity to cleave the 5'-terminated strand flanking the gap or the ssDNA. EXO1 and DNA2-WRN/BLM specifically resect the 5' end of the gap independent of MRN-CtIP. This resection process alters …

Marine Heat Waves Effects On Rocky Intertidal Ecosystem Functioning, Skyler Peterson, S. Schneider, C.L. Webb, A. Leighton

Undergraduate Research Symposium Posters

Severe heat waves may cause long-term changes in marine ecosystems and are expected to increase in magnitude and frequency. Here we use surveys from six localities across a six-year interval (2013-2019) to investigate the effects of heat waves on the functional diversity of rocky intertidal ecosystems (Vancouver Island, Canada).

Functional diversity, the number and types of functions performed by organisms, influences ecosystem stability and health. To assess changes in functional diversity we used analyses of biological traits. Biological traits are measurable properties of organisms that describe their morphology, physiology, and behavior. We quantitatively assessed changes in functional diversity in response …

Enhanced Design Of Lipid Nanoparticles Toward Cell-Specific Gene Delivery, Anna M. Bell

Honors Capstone Projects and Theses

No abstract provided.

Strategies For Identifying Entrepreneurial Opportunities In Small Business It Consulting, Oluwatosin Ogun

Walden Dissertations and Doctoral Studies

The lack of effective strategies for identifying opportunities threatens the survival and growth of small IT consulting businesses. Without these strategies, owners risk struggling to sustain their businesses in a highly competitive market. Grounded in the theory of planned behavior, the purpose of this qualitative pragmatic inquiry was to explore strategies that IT consulting small business owners use to identify business opportunities for growing their business. The project participants were six IT consulting small business owners in the Mid-Atlantic region of the United States who led or significantly contributed to successfully recognizing business opportunities in firms at least 5 years …

Staff Education To Improve Staff Knowledge And Attitudes Regarding Aggressive Patient Behaviors, Roya Khoshbakht

Walden Dissertations and Doctoral Studies

Aggressive behavior among psychiatric patients threatens safety and quality in healthcare, causing physical and psychological harm to staff and contributing to burnout and high turnover rates. This Doctor of Nursing Practice (DNP) project addressed gaps in staff knowledge and attitudes, particularly among nurses, regarding aggression management and de-escalation techniques through a comprehensive education program on the causes of aggression, warning signs, and effective strategies. The evidence-based practice (EBP) question was, Will staff education on evidence-based strategies for preventing and managing aggressive behavior improve staff knowledge and attitude? Project team members used the Johns Hopkins Evidence-Based Practice (JHEBP) model to develop …

Art Historical Research Of The Intercession Of Christ And The Virgin, Attributed To Lorenzo Monaco, Lauren Watts

Curiosity

This research examines The Intercession of Christ and the Virgin, a painting attributed to Lorenzo Monaco. The painting is tempera on canvas, and is housed at the Metropolitan Museum of Art. This research looks at the painting through a phenomenological lens in context of its original site, iconographic themes, and feminine spirituality. The research posits that the painting’s intercessory figures—Christ and the Virgin Mary—construct a narrative amplifying themes of feminine spirituality, challenging the misogynistic structures of quattrocento Florentine ecclesiastical culture while remaining within the bounds of contemporary orthodoxy. Studying the painting at Metropolitan Museum facilitated in-depth visual analysis, supplemented by …

2025 Lynn University Commencement Program, Lynn University

Commencement Programs

The commencement exercises program for Lynn University's class of 2025. The ceremony, held on Friday, May 2, 2025 at the Palm Beach Convention Center, included all graduating students (undergraduates and graduates).

The commencement speaker was Brandon Busteed, CEO of BrandEd.

Artificial Intelligence-Based Virtual Staining Platform For Identifying Tumor-Associated Macrophages From Hematoxylin And Eosin-Stained Images, Arpit Aggarwal, Mayukhmala Jana, Amritpal Singh, Tanmoy Dam, Himanshu Maurya, Tilak Pathak, Sandra Orsulic, Kailin Yang, Deborah Chute, Justin A Bishop, Farhoud Faraji, Wade M Thorstad, Shlomo Koyfman, Scott Steward, Qiuying Shi, Vlad Sandulache, Nabil F Saba, James S Lewis, Germán Corredor, Anant Madabhushi

Faculty, Staff and Students Publications

Background: Virtual staining is an artificial intelligence-based approach that transforms pathology images between stain types, such as hematoxylin and eosin (H&E) to immunohistochemistry (IHC), providing a tissue-preserving and efficient alternative to traditional IHC staining. However, existing methods for translating H&E to virtual IHC often fail to generate images of sufficient quality for accurately delineating cell nuclei and IHC+ regions. To address these limitations, we introduce VISTA, an artificial intelligence-based virtual staining platform designed to translate H&E into virtual IHC.

Methods: We applied VISTA to identify M2-subtype tumor-associated macrophages (M2-TAMs) in H&E images from 968 patients with HPV+ oropharyngeal squamous cell …

Longitudinal Profiling Of Circulating Tumor Dna Reveals The Evolutionary Dynamics Of Metastatic Prostate Cancer During Serial Therapy, Yuehui Zhao, Naveen Ramesh, Ping Xu, Emi Sei, Min Hu, Shanshan Bai, Patricia Troncoso, Ana M Aparicio, Christopher J Logothetis, Paul G Corn, Nicholas E Navin, Amado J Zurita

Faculty, Staff and Student Publications

Treatment decisions in metastatic castration-resistant prostate cancer are mostly guided by clinical variables, but efforts to molecularly monitor the disease remain hampered by challenges in acquiring tumor tissue repeatedly. In this study, we simultaneously profiled the genome copy number and exome in longitudinal plasma circulating tumor DNA (ctDNA) acquired before, during, and upon progression to serial treatments with androgen signaling inhibitors and taxane chemotherapy from 60 patients with metastatic castration-resistant prostate cancer (2-10 samples per patient). The genomic data were used to delineate the clonal substructure and evolutionary dynamics of each patient, and an evolutionary dynamic index was developed to …

Dying Young, Brianne L. Overton

Dissertations

Death, often perceived as a disruption to daily life, is an inevitable part of the human experience. While older adults may develop familiarity with mortality through the collective experience of personal and familial losses, young adults generally have limited exposure to death. Despite this, young adults, defined as individuals aged 20 to 34, represent approximately 20% of the U.S. population (U.S. Census Bureau, 2020), and the mortality rate within this age group has been rising over the past decade (Woolf & Schoomaker, 2019). Death among young adults is often overlooked in societal narratives and medical research, which primarily focus on …

Epidemiology, Risk Factors And Clinical Outcome Of Candidaemia: A Decade-Long Experience At A University Hospital In Oman, Tasneem Al Salmani, Asmaa S. Mahdi, Abdullah Balkhair, Turkiya Al Siyabi, Hashim Ba Wazir

Sultan Qaboos University Medical Journal

Objectives: This study aimed to examine a decade-long epidemiology of candidaemia to explore the risk factors associated with it, and describe its clinical outcomes in hospitalised adult patients. Methods: This retrospective observational study was conducted at Sultan Qaboos University Hospital, Muscat, Oman. Adult patients with candidaemia hospitalised between January 2007 and December 2016 were included. The hospital infection control department surveillance registry on bloodstream infections (BSIs) was used to identify Candida BSIs. Patients' electronic medical records were searched for demographic characteristics, clinical risk factors and outcomes. Results: Candida albicans was the most common Candida species among 152 …

Incidence And Predictors Of Permanent Pacemaker Implantation Following Transcatheter Aortic Valve Replacement: Two-Centre Experience From Oman, Mohamed N. Al Rawahi, Adil Al Kindi, Ahmed Al Yarubi, Ahmed Shams, Adil Al Riyami, Hatim Al Lawati, Ahmed El Said, Mohamed Al Riyami, Khalid Al Saidi, Ismail Al Abri, Najib Al Rawahi, Abdullah Al Ismaili, Fahad Al Kindi, Muhammad A. Sadiq, Sunil K. Nadar

Sultan Qaboos University Medical Journal

Objectives: This study aims to evaluate the predictors and rate of permanent pacemaker (PPM) implantation among patients undergoing transcatheter aortic valve replacement (TAVR) at two institutions in Oman. TAVR has become the standard of care for patients with severe aortic stenosis at high risk for surgery. However, it is associated with a high PPM implantation rate. Methods: This was a retrospective study involving all patients undergoing TAVR at two leading cardiac centres in Oman over seven years, from May 2013 to September 2020. We included patients who survived the procedure to discharge and those who were followed-up for …

Genomic Characterization Of High-Grade Serous Ovarian Carcinoma Reveals Distinct Somatic Features In Black Individuals, Katherine A Lawson-Michod, Jeffrey R Marks, Lindsay J Collin, David A Nix, Natalie R Davidson, Chad D Huff, Yao Yu, Aaron Atkinson, Courtney E Johnson, Lucas A Salas, Lauren C Peres, Casey S Greene, Joellen M Schildkraut, Jennifer A Doherty

Faculty, Staff and Student Publications

Black individuals experience worse survival after a diagnosis of high-grade serous ovarian carcinoma (HGSC) than White individuals and are underrepresented in ovarian cancer research. To date, the understanding of the molecular and genomic heterogeneity of HGSC is based primarily on the evaluation of tumors from White individuals. In the present study, we performed whole-exome sequencing on HGSC samples from 211 Black patients to identify significantly mutated genes and characterize mutational signatures, assessing their distributions by gene expression subtypes. The occurrence and frequency of somatic mutations and signatures by self-reported race were compared with historic data from The Cancer Genome Atlas …

Staphylococcal Scalded Skin Syndrome (Ssss): A Short Review, Anmar M. K. Al-Maamori, Zahraa M. Altaee, Noor Mahommoud Naj, Rafflaa S. Hussien

Hilla University College Journal For Medical Science

Staphylococcal scalded skin syndrome (SSSS) is a skin problem characterized by the formation of blisters. It primarily affects newborns, young children, and adults with pre-existing medical conditions. This condition is caused by an infection from the bacteria Staphylococcus aureus. The cause of this case is the breakdown of desmoglein-1, a protein in the skin, due to the action of exotoxins. This results in the outer layer of the skin's cells peeling off. The SSSS is caused by the dissemination of toxins throughout the body, leading to a widespread rash and a severe manifestation of symptoms. Staphylococcal scalded skin syndrome …

Correlation Between Varicella-Zoster Virus Infection And Cancer Development: A Comprehensive Analysis, Fernando Cisneros, Blake Martin, Shizue Mito

School of Medicine Publications

Varicella-zoster virus (VZV) is a highly prevalent pathogen primarily recognized for causing chickenpox during primary infection, and herpes zoster (HZ), also known as shingles, upon reactivation. While post-infectious complications of VZV, such as encephalitis, pneumonia, and post-herpetic neuralgia, are well-established, recent large population-based studies suggest that HZ may increase the risk of occult cancers. This has sparked discussions on the need for cancer screening in patients with HZ to improve early diagnosis and prognosis. However, the specific types of cancers most strongly associated with VZV reactivation have not been systematically identified, and the subsequent cancer risk remains inconclusive. Emerging evidence …

Optimising Non-Cycloplegic Screening Strategies For Early Detection Of Pre-Myopia And Myopia In Young Children, Síofra Harrington, Michael Moore, James Loughman, Ian Flitcroft, Veronica O'Dwyer

Articles

Purpose: Early detection of myopia is essential to delay its onset and progression. Pre-myopia, defined by an inadequate hyperopic reserve, increases myopia risk in childhood. However, effective screening methods remain limited. This study aimed to develop practical non-cycloplegic screening methods for pre-myopia and myopia in 6- to 7-year-olds to support earlier interventions.

Methods: This cross-sectional study of 621 Irish schoolchildren (mean age: 7.12 ± 0.45 years; 51.8% boys) assessed uncorrected distance visual acuity (UDVA). Cycloplegic spherical equivalent refraction (SER) classified refractive status (myopia: SER ≤ −0.50D; pre-myopia: SER > −0.50 ≤ 0.75D). Pre- and post-cycloplegic SER were measured using the Welch …

A Tobacco Treatment Training Program Expands The Number Of Healthcare Providers Trained To Use Evidence-Based Practices And Methods, Alex Hurst, Maher Karam-Hage, Jennifer Cofer, Mehwish Javaid, Diane Beneventi, Janice Blalock, Tiffany Winter, Paul M. Cinciripini, Ernest Hawk

Advances in Cancer Education and Quality Improvement

Tobacco-use disorder remains a global health challenge, despite declining rates, and is a leading cause of morbidity and mortality, especially among special populations that disproportionally do not have access to comprehensive treatment services. The lack of trained providers and slow dissemination of new tobacco treatment knowledge are obstacles to accessing comprehensive evidence-based treatments. Less is known about the impact of specialized training programs on healthcare professionals' ability to effectively treat tobacco use. This paper reports on the evaluation of the tobacco treatment training program, training methodology, and the demographics of the specialists trained.

MD Anderson’s Tobacco Treatment Training Program (TTTP) …

“Jesus Is Integrated”: The Black Sermon As Rhetorical Exercise In Radical Worldmaking, Theon E. Hill, Tyran Laws

Sermon Studies

This essay analyzes Benjamin Mays' 1954 sermon to the World Council of Churches to argue that Black preaching offers a radical response to perspectives that portray racial justice advocacy as antithetical to the Christian Gospel. Through a close reading of Mays' sermon, the authors contend that Mays employs an implicit ontological metaphor, “Jesus is integrated,” to position racial integration and justice not only as civic responsibilities but as Christian duties. The sermon disrupts hegemonic ideologies that marginalize others, mobilizes the organizing power of the church, and positions churches as spaces for radical worldmaking around race. By drawing on metaphor theory, …

Impact Wv: Adapting Patient Navigation Models Of Care To Improve Services To Caregivers Of Infants Exposed To Substances In Utero, Charlotte S. Workman Bsms, Ccrp, Lesley Cottrell, Mawyah Bashatah, Aisha Hashmi, Kayla Richard, Poornima Murthy, Susan Harrison, Ashleigh Mckinsey, Amy Bott, Sydney Leary, Cody Smith, Melina Danko

Journal of Appalachian Health

Patient navigation (PN) is a healthcare service delivery model designed to partner clients with service providers to reduce challenges to health care, as well as to improve access to care by identifying opportunities to coordinate services for clients. The IMPACT WV project at the West Virginia University Center for Excellence in Disabilities (WVU CED) implemented PN models of care for caregivers of infants exposed to substances in utero. The purpose of this program was to reduce burden for potentially at-risk families and improve caregiver and infant outcomes as a result. To understand the processes that influence the implementation of PN …

Determinants Of Willingness To Share Wearable Health Data With Health Care Providers In Appalachian Populations: An Exploratory Study, Gilbert Munoz Cornejo, Joonghee Lee

Journal of Appalachian Health

Background: Wearable health devices capture metrics (e.g., physical activity, ECG, sleep) that can enhance care when shared with providers. Yet, willingness to share wearable data may differ in Appalachia, where chronic disease burdens, mistrust, and limited infrastructure pose unique challenges.

Objective: This study explored (1) which sociodemographic, health, and digital behaviors correlate with willingness to share wearable data and (2) how these insights can guide region-specific interventions in Appalachia.

Methods: We analyzed 320 Appalachian respondents from the Health Information National Trends Survey (HINTS 6). Descriptive statistics and logistic regression models examined willingness to share wearable data. Because of small cell …

Are Large Language Models Conscious?, Judah Hays

Undergraduate Research and Engagement Symposium

This work evaluates the possibility of a conscious large language model (LLM) under the parameters of Integrated Information Theory (IIT). First, we examine the methodology behind IIT as a scientific theory of consciousness. Second, we detail the system requirements for consciousness under IIT. Third, we examine the basic architecture of modern LLMs. Finally, we subject the network architecture of current LLMs to the system requirements of IIT, evaluating each “physical postulate” IIT espouses individually. We conclude that LLMs meet none of IIT’s requirements for a physical system to be conscious, and briefly discuss the implications for future research.

Mindfulness-Based Interventions For Drug Addiction: Insights From The Predictive Processing Framework, Sera Parag Gandhi

Butler Journal of Undergraduate Research

Drug addiction, a globally prevalent and burdensome psychiatric disorder, poses a significant challenge to conventional treatments, necessitating a deeper understanding of its mechanisms and exploration of alternative approaches for effective intervention. Despite initial success in research trials, mindfulness-based interventions (MBIs) have been met with skepticism due to a lack of comprehensive understanding of their underlying treatment mechanisms. The present paper argues that the predictive processing (PP) framework can provide unifying accounts of addiction and MBIs, and that a closer examination of the underlying mechanisms involved in both the disorder and the treatment can offer important insights for intervention and recovery. …

Old, Similar, Or New: Hippocampal-Dependent Pattern Separation Performance In Fibromyalgia, Ashley Laplant

Butler Journal of Undergraduate Research

In addition to chronic musculoskeletal pain, people with fibromyalgia often have memory deficits. Pattern separation is a neurological process that may be related to the memory deficits observed in people with fibromyalgia. Greater hippocampal activation has been associated with pattern separation deficits outside of fibromyalgia and has been observed in people with fibromyalgia; however, the relationship between hippocampal activation and pattern separation deficits in people with fibromyalgia is still unclear. This study thus aimed to examine the relationship between hippocampal activation and pattern separation in fibromyalgia. We hypothesized that participants with fibromyalgia would have worse pattern separation performance and greater …

A Grotesque Beacon Of God’S Grace In “A Temple Of The Holy Ghost”, Shayleigh Pape

Butler Journal of Undergraduate Research

The faith of Flannery O’Connor, a Catholic writer, creates a unique tension within her short stories, between the grotesque aspects of the natural world and the sanctity of the divine. Her work “A Temple of the Holy Ghost” exemplifies this tension as a young female narrator encounters an unexpected transformative experience after her two cousins see a “hermaphrodite”[1] at a local “freak show.” In “A Temple of the Holy Ghost,” Flannery O’Connor employs the hermaphrodite character as a vessel for the narrator to explore the mysterious virtue of purity as she matures both literally and spiritually. In doing so, O’Connor …

Grey’S Anatomy College Of Medicine: How Modern Medical Dramas Have Contributed To Patient Healthcare Anxiety, Haylee R. Tilley

Butler Journal of Undergraduate Research

Having run for nearly 20 seasons averaging over 37 million viewers, it is no surprise that Grey’s Anatomy has been a captivating medical melodrama, one that has sustained popularity in an ever-changing society during its nearly two decades in production. Modern medical dramas have sparked popularity because of their captivating themes of suffering and healing: the modern happy ending or, in this case, cure. With a comprehensible mix of medical jargon and lighthearted drama, audiences around the globe are claiming an MD-level proficiency due to their dedication and engagement in this Shonda Rhimes award-winning series. The increase in patients made …