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Mentored Ministry - Two, Joy Ireland

Syllabi

No abstract provided.

Mentored Ministry - Two, Joy Ireland

Syllabi

No abstract provided.

Advancing Butterfly Systematics Through Genomic Analysis, Jing Zhang, Qian Cong, Jinhui Shen, Leina Song, Nick V. Grishin

The Taxonomic Report of the International Lepidoptera Survey

Within the framework of an ongoing comparative genomic study of global butterfly diversity, we construct phylogenetic trees combining all protein-coding genes assembled from the whole genome shotgun data. When viewed in the context of current taxonomy and phenotypic knowledge, the genome-wide phylogeny points to further advances in butterfly systematics, which are presented here. We assign major clades with comparable levels of genetic divergence to the same taxonomic rank and apply criteria involving relative population divergence and gene flow to define species boundaries. As a result, one genus, 13 subgenera, 62 species, and five subspecies are proposed as new (type species …

The Nitrogen-Vacancy-Nitrogen Color Center: A Ubiquitous Visible And Near-Infrared-Ii Quantum Emitter In Nitrogen-Doped Diamond, Brett C. Johnson, Mitchell O. De Vries, Alexander J. Healey, Marco Capelli, Anjay Manian, Giannis Thalassinos, Amanda N. Abraham, Harini Hapuarachchi, Tingpeng Luo, Vadym N. Mochalin, Jan Jeske, Jared H. Cole, Salvy Russo

Chemistry Faculty Research & Creative Works

Photoluminescent defects in diamond, such as the nitrogen-vacancy (NV) color center, are at the forefront of emerging optical quantum technologies. Most emit in the visible and near-infrared spectral region below 1000 nm (NIR-I), limiting their applications in photonics, fiber communications, and biology. Here, we show that the nitrogen-vacancy-nitrogen (N2V) center, which emits in the visible and near-infrared-II (NIR-II, 1000-1700 nm), is ubiquitous in as-synthesized and processed nitrogen-doped diamond, ranging from bulk samples to nanoparticles. We demonstrate that N2V is also present in commercially available state-of-the-art NV diamond sensing chips made via chemical vapor deposition (CVD). In high-pressure high-temperature (HPHT) diamonds, …

Investigating Genes Associated With Oncogenic Pka Activity In Fibrolamellar Carcinoma, Ananya Singh

Undergraduate Honors Theses

Fibrolamellar Carcinoma (FLC) is a rare liver cancer, predominantly affecting younger individuals with no history of primary liver disease. As FLC comprises only < 1% of all liver tumors, our understanding of its development and treatment are extremely limited. All clinical cases of FLC contain a specific DNAJB1-PRKACA fusion. The mutation produces an oncogenic form of Protein Kinase A (PKA), known as DNAJ-PKAc, with enhanced binding activity compared to wild-type PKA. Specifically, DNAJ-PKAc interacts with different substrates than wild type PKA, affecting downstream signaling pathways to promote cancer development. However, the genetic dependencies that result from oncogenic DNAJ-PKAc signaling are not known. In this paper, I will show the process of performing a genome-wide CRISPRi screen in AML12DNAJ-PKAc cells to identify genes associated with oncogenic DNAJ-PKAc signaling. I will detail the preliminary benchmarking experiments, the screening process, the preparation of genomic DNA for sequencing, and the ongoing validation of screen results. I anticipate that this project will discover genes whose knockdown inhibits oncogenic DNAJ-PKAc signaling and reduces cell growth exclusively in the presence of PKA. The identified genes could represent potential therapeutic targets for cancer drugs that inhibit FLC progression. However, additional research is necessary to characterize their interactions with DNAJ-PKAc and their specific role in FLC development.

Evaluating The Protective Role Of Epo-R76e In Oxidative Stress-Induced Cell Death In Retinal Pigment Epithelium, Jemima Alam

USF Tampa Graduate Theses and Dissertations

Age-Related Macular Degeneration (AMD) causes dysfunction and loss of Retinal pigment epithelium (RPE). The degeneration and death of RPE cells causes irreversible visual impairment. Oxidative stress is a widely accepted triggering factor for RPE dysfunction. A hormone called Erythropoietin (EPO) is responsible to facilitate the erythropoiesis process, prevents progenitor cells from apoptosis and protects photoreceptors downregulating retinal degeneration. In this study, we aim to elucidate the role of EPO in downregulating oxidative stress in RPE. It is hypothesized that EPO will have a positive impact on suppressing oxidative stress and ferroptosis-mediated RPE degeneration due to its vital role in apoptosis …

Compositions And Methods For Weight Loss Maintenance, Dominic Paul D'Agostino, Angela Marie Poff, Andrew Paul Koutnik

USF Patents

Compositions and methods for inducing weight loss, fat loss, maintaining weight or fat loss, suppressing appetite, reducing calorie intake, reducing weight gain, decreasing feed efficiency, increasing metabolic inefficiency, increasing energy expenditure, increasing thermogenesis and/or resetting metabolic set are disclosed. The compositions have a therapeutically effective amount of a ketogenic supplement, e.g., a ketone diesters, such as R,S 1,3-butanediol diacetoacetate. Compositions of one or more beta-hydroxy butyrate, beta-hydroxybutyrate salt, and medium chain triglycerides are also disclosed.

Proteomic Investigation Of Aging And Omega-3 Supplementation In Rat Skeletal Muscle, Francesco Maria Del Re, David W. Russ, Kalina P. Dimova, Stylianos P. Scordilis

Biological Sciences: Faculty Publications

Aging is a pan-organ process with an intricate and multimodal nature. Deciphering the aging phenomenon is complex, yet recent attention is analyzing the potential benefits of non-invasive life adjustments to achieve healthy aging. Omega-3 polyunsaturated fatty acids (FA) have emerged as promising nutraceuticals for a plethora of different medical conditions. In the current study we conducted an in-depth, bottom-up, global, shotgun proteomic study (LC-MS/MS) investigating both the effects of aging on skel- etal muscle and the potential alterations due to ω-3 FA. Sprague Dawley rats were fed different diets and divided into four groups (n = 5 per group): adult …

Progress Towards The Asymmetric De Novo Synthesis Of Tetracyclic Triterpenoids: Cucurbitanes, Lanostanes, And Cardiotonic Steroids, Andrea Rachel Bucknam

Dartmouth College Ph.D Dissertations

The complex molecular scaffolds of tetracyclic triterpenoid natural products continue to inspire novel chemical reactions. Steroid-based drugs have contributed significantly to human health in the past century, demonstrating a broad range of therapeutic applications including anti-viral, anti-inflammatory, and anti-cancer activities. However, the efficient and flexible synthesis of tetracyclic triterpenoids remains an unsolved problem. Despite numerous reports of medically relevant bioactivity associated with cucurbitanes, lanostanes, and cardenolides, exploration of their pharmaceutical promise has been limited by a lack of access to molecules bearing their requisite carbon frameworks. Prior to the initiation of my doctoral research, there were no reported syntheses of …

Enduring Words: Resistance Through Sculptural Libraries, Alex C. Jacobs

LSU Master's Theses

Enduring Words: Resistance Through Sculptural Libraries is a thesis exhibition that challenges book censorship by transforming reading into a public and defiant act. Through direct sculptural interventions, the exhibition confronts cultural erasure, questions who controls the literature we consume, and highlights the role of art in safeguarding contested knowledge. This thesis focuses on a current body of exhibited sculptural works that embody the tension between access and erasure. These installations engage with the viewer by using a lighthouse built of books and a speech-bubble-shaped library embedded with censored texts. They do not quietly mourn the suppression of knowledge; they confront …

Atrapado En Limbo: Fronteras, Anthropologists, And Translation In Contexts Of The Missing And Unidentified At The México-Us Border, Aliya M. Godoy

LSU Master's Theses

The Missing Migrants Project reports that in the past 11 years, 11,138 missing migrants have been recorded in the Americas, with 6,512 of those migrants situated at the México-United States (US) border (Migration Within the Americas, n.d.). The crisis of missing and unidentified migrants at the México-US border is a topic of great discussion in the field of anthropology, and specifically in discourses of humanitarian forensic anthropological work. This research addresses the crisis at the border, expanding on the scholarship of family agency and language barriers between Spanish-speaking communities and medicolegal actors by investigating the role of anthropologists …

Exploring Sense Of Belonging Among Neurodivergent Students In Higher Education, Mollie Rubinstein

Theses and Dissertations

Positive academic experiences for university students are hard to come by when non-academic experiences are negative (Gidley et al., 2010; Sandhu et al., 2022; Tinto, 1975). This issue has become increasingly important for neurodivergent students in higher education as more of this population is enrolling in university (Cox et al., 2002; Edwards et al., 2022; Gurbuz et al., 2018; Lubin & Brooks, 2021; Izzo et al., 2008; Mamiseishvili & Koch, 2011; Yssel et al., 2016). The purpose of this study is to listen to neurodivergent student voices to learn about their university experience and sense of belonging. Research questions include: …

Relations Among Racism-Related Stress, Anxiety, Spirituality And Mindfulness In Black Sexual Minority Men, Kainaat Anwar

Theses and Dissertations

Abstract Kainaat Anwar RELATIONS AMONG RACISM-RELATED STRESS, ANXIETY, SPIRITUALITY AND MINDFULNESS IN BLACK SEXUAL MINORITY MEN 2021-2022 Jonathan Mathias Lassiter, Ph.D. Master of Arts in Clinical Psychology Black sexual minority men’s experiences of racism and anxiety have not been documented extensively in the literature. There is also a paucity of research exploring protective factors that can mitigate the effects of racial stress on anxiety in BSMM. Therefore, this study looked at the potential moderating effects of spirituality and mindfulness on the relationship between racism-related stress and anxiety in BSMM. Participants completed measures regarding socio-demographics, racism-related stress, anxiety symptoms, mindfulness and …

Exploring Virtual Group Acceptance And Commitment Therapy Format On Participation Of Parents Of Children With Autism, Abigail Owen

Theses and Dissertations

Parents of children with autism spectrum disorder (ASD) and co-occurring challenging behavior (e.g., aggression, self-injury, property destruction) experience higher rates of anxiety, depression, and poor overall well-being. Acceptance and commitment therapy (ACT), which encourages psychological flexibility, is an effective intervention for addressing well-being in this population when presented in various formats. Despite its potential to increase accessibility and improve social connectedness for parents, time commitment may impact parents’ willingness to participate in virtual ACT group interventions. The current study examined parents’ participation in and perceived acceptability of 6 hrs of virtual ACT groups in three different formats: a 6-hr workshop, …

Optogenetic Activation Of Cortical Microglia Promotes Neuronal Activity And Pain Hypersensitivity, Min-Hee Yi, Yi Liu, Yong U Liu, Jinkyung Lee, Priyanka Hanumaihgari, Sebastian Parusel, Dale B Bosco, Lingxiao Wang, Jiaying Zheng, Wu Shi, Lattawat Eauchai, Supin Chompoopong, Christine L Hunt, Long-Jun Wu

The Brown Foundation: Institute of Molecular Medicine

Chronic pain following peripheral nerve injury is accompanied by increased neuronal activity in the somatosensory cortex. However, whether and how cortical microglia contribute to these changes is less understood. To this end, we applied an optogenetic strategy to specifically target cortical microglia and investigate their function in behavioral pain sensitization. We found that optogenetic activation of microglia in the primary somatosensory cortex (S1) via red-activated channelrhodopsin (ReaChR) triggered pain hypersensitivity and affective-motivational responses in mice. Remarkably, S1-targeted optogenetic stimulation increased microglial landscape changes and ATP release. In addition, optogenetic stimulation altered the microglial proteomic profile, upregulated neuronal c-Fos expression, and …

Mechanical Design Procedure And Modelling For 8-Dof Upper Limb Rehabilitation Robotic, Mohanad Al-Asady, Ayad Murad Takhakh

Baghdad Science Journal

Treating muscular medical injuries that impede movement of the human body has become of wide interest because of the importance it holds for improving the lives of people who are exposed to this type of injury. During the past years, several studies have attempted to create a robot that works to rehabilitate the injured upper limbs of the human body. In this research, a curriculum for the procedures will be developed for designing a medical robot by displaying the volumetric tolerances for this robot according to the age and weight of the injured person and the weight and volumetric constants …

The Nih-Moderna Public-Private Partnership: A New Contractual Model For Securing Innovation, Karen Sandrik

Texas A&M Law Review

The public–private partnership between the National Institutes of Health (“NIH”) and Moderna was considered “one of the few bright spots of the pandemic.” Yet as the NIH–Moderna collaboration progressed at an unprecedented pace, going from vaccine development to FDA authorization in nine months, cracks began to appear in the partnership. Public trust in vaccinations wavered, multiple patent disputes arose, and global frustration erupted over Moderna’s lack of commitment to equitable access to the vaccine that was largely paid for by U.S. taxpayers. This Article argues that the parties’ contractual agreements did shockingly little to support or, indeed, set up the …

The National Bioengineered Foods Disclosure Standard & Absence Claims: The Cost Of The Butterfly, Cahlen Cheatham

Texas A&M Law Review

The National Bioengineered Foods Disclosure Standard (“NBFDS”) is a step in the right direction in providing a uniform labeling standard for genetically engineered organisms (“GEOs”) that meet the statutory definition of “bioengineered.” Importantly, the law provides a consensus that GEOs are safe and that labeling standards should be uniform across the United States. However, the law largely fails to settle the broader debate and the issues associated with GEO labeling. Namely, the NBFDS’s use of the term “bioengineered” causes additional confusion, and terms like “genetic modification” and “genetic engineering” remain ambiguous. Furthermore, the Final Rule promulgated by the U.S. Department …

The Aquatic Symbiosis Genomics Project: Probing The Evolution Of Symbiosis Across The Tree Of Life, Matthew Berriman, Victoria Mckenna, Roxanne Beinart, John M. Archibald, Ute Hentschel, Michael N. Dawson, Jose V. Lopez, Patrick J. Keeling, Jillian M. Petersen, Anne W. Thompson, Multiple Addiional Authors

Biology Faculty Publications and Presentations

We present the Aquatic Symbiosis Genomics Project, a global collaboration to generate high quality genome sequences for a wide range of eukaryotes and their microbial symbionts. Launched under the Symbiosis in Aquatic Systems Initiative of the Gordon and Betty Moore Foundation, the ASG Project brings together researchers from across the globe who hope to use these reference genomes to augment and extend their analyses of the dynamics, mechanisms and environmental importance of symbioses. Applying large-scale, high-throughput sequencing and assembly technologies, the ASG collaboration will assemble and annotate the genomes of 500 symbiotic organisms – both the “hosts” and the microbial …

Prevalence And Determinants Of Poor Glycemic Control In Adult Diabetic Patients In A Nigerian Tertiary Hospital, Jideuma Ikenna Egwim, Hope Igbonagwam, Adanna Vivian Egwim, Hadi Danawi, Ifeoma Ogugua

Journal of Social, Behavioral, and Health Sciences

Diabetes mellitus (DM) is a significant public health concern globally and in Owerri, southeast Nigeria. The deleterious effects of diabetes have been linked to poor glycemic control. According to the International Diabetes Federation, poor glycemic control is reflected in glycosylated hemoglobin (HbA1c) levels > 7.0%, which are associated with substantial morbidity and mortality. Studies have shown a dramatic rise in diabetic complications in Nigeria, particularly in Owerri. However, evidence is lacking regarding the specific risk factors associated with poor glycemic control among DM patients in Owerri. This study assessed the predictors of poor glycemic control in a hospital setting in Owerri. …

The Risk Of Interstitial Lung Disease In Psoriatic Arthritis Versus Psoriasis: A Retrospective Nationwide Database Analysis (2014-24), Andrew Engel, Christopher Xie, Ross Summer, Giorgos Loizidis

Division of Pulmonary, Allergy, and Critical Care Medicine Faculty Papers

OBJECTIVES: Psoriasis (PsO) is a systemic autoimmune disease primarily characterized by erythematous plaques on the skin. While extra-dermal manifestations like psoriatic arthritis (PsA) are well recognized, data linking PsO to interstitial lung disease (ILD) remain limited. This study aimed to evaluate whether patients with PsA have a higher risk of developing ILD compared with patients with PsO.

METHODS: A retrospective analysis of the TriNetX US database (2014-24) was performed. Adult patients with PsO or PsA treated with systemic immunosuppressive medications were included, excluding those with other autoimmune diseases. ILD risk in PsO and PsA cohorts was compared with a reference …

Narrative Counseling, Tapiwa Mucherera

Syllabi

No abstract provided.

"I'M Strictly T4t": Trans For Trans Connections In The Contemporary, Madi Lou Alexander

Dissertations and Theses

Trans for trans relationships, referred to as "t4t", are a relationship type specific to trans individuals. Linguistically originating from Craigslist personal ad spaces, t4t indicates a trans person seeking out and engaging with another trans person. The scope of relationships under the t4t label are often largely associated with sexuality and dating scenes due to their conception in online cruising spaces. Through qualitative analysis, I assess the range of what t4t relationships are through 30 in-depth interviews with participants identifying as trans. This work moves away from focusing on the deficits of holding a marginalized identity, instead aiming to center …

Antibiofilm Properties Of 4-Hydroxy-3-Methyl-2-Alkenylquinoline, A Novel Burkholderia-Derived Alkaloid, Mckinley D Williams, Taylor R Sweeney, Sabrina Trieu, Ravi Orugunty, Abdelahhad Barbour, Fereshteh Younesi, Michael Glogauer, Nopakorn Hansanant, Ronald Shin, Shi-En Lu, Kevin Cao, Abraham Tenorio, Sigmund J Haidacher, Anthony M Haag, Thomas D Horvath, Leif Smith

Faculty, Staff and Students Publications

Biofilms are an important colonization mechanism employed by several microbial species to better establish themselves and monopolize the acquisition of resources across different environs. Some bacteria have evolved specialized metabolites that, when secreted, disrupt the formation and stability of biofilms generated by competing heterospecies, providing the producing organism with an ecological advantage. Soil-derived species are probable candidates for the identification of such compounds, given the intense level of competition that occurs within the terrestrial ecosystem. The MS14 strain of Burkholderia contaminans isolated from soil in Mississippi has previously been shown to produce antimicrobial compounds like occidiofungin and ornibactin. In this …

Tumour-Associated Macrophage Infiltration Differs In Meningioma Genotypes, And Is Important In Tumour Dynamics, Ting Zhang, Claire L. Adams, Gyorgy Fejer, Emanuela Ercolano, Jonathan Cutajar, Juri Na, Felix Sahm, C. Oliver Hanemann

Peninsula Medical School

Background: Meningiomas are the most common primary intracranial tumours, with clinical behaviours ranging from benign to highly aggressive forms. The World Health Organisation classifies meningiomas into various grades, guiding prognosis and treatment. While surgery is effective for low-grade meningiomas, certain grade 1 tumours, as well as grade 2, 3, and recurrent cases are more aggressive and require new therapeutic approaches. Immunotherapy shows promise, with early-stage clinical trials demonstrating encouraging results. The tumour microenvironment (TME), particularly tumour-associated macrophages (TAMs), plays a pivotal role in tumour progression. TAMs influence tumour growth, metastasis, and immune evasion. However, their role in meningiomas, especially in …

Stationary Distribution Of Stochastic Seirs Epidemic Model With Saturated Incidence Rate And Saturated Treatment Function, S. Saravanan, C. Monica

Baghdad Science Journal

This investigation aims to enhance and broaden the mathematical model that governs a dynamic stochastic SEIRS (Susceptible, Exposed, Infective, and Recovery) epidemic. This complex model integrates crucial components, including a saturated incidence rate and saturated treatment function, which are fundamental in molding epidemic dynamics. The objective is to explore the presence and uniqueness of a positive global solution through the application of a meticulously designed Lyapunov function, facilitating a more profound analysis of the intricacies of the systems. This analytical framework enables us to uncover the interactions among disease transmission, treatment dynamics, and stochastic influences. This theoretical framework assumes that …

Darkening Of The Global Ocean, Thomas Davies, Tim Smyth

School of Biological and Marine Sciences

The photic zones of the oceans – where sunlight and moonlight drive ecological interactions - are one of the most productive habitats on the planet, and fundamental to the maintenance of healthy global biogeochemical cycles. Ocean darkening occurs when changes in the optical properties of the oceans reduces the depth to which sufficient light penetrates to facilitate biological processes guided by sunlight and moonlight. We analysed a 9km resolution annual time series of MODIS Aqua’s diffuse attenuation coefficient of light at 490nm [Kd(490)] to quantify whether the oceans have darkened over the last twenty years, and the impact of this …

Allele-Specific Vitamin D Receptor Binding Is Associated With Pediatric-Onset Multiple Sclerosis, Defne Yilmaz, Soe Mar, Et Al.

2020-Current year OA Pubs

BACKGROUND AND OBJECTIVES: The genetic basis of adult-onset multiple sclerosis (MS) is well-studied, but less is known about pediatric-onset MS (pedMS), comprising approximately 5% of all MS onsets. Mendelian randomization (MR) studies have demonstrated evidence for a causal association between MS and both 25-hydroxyvitamin D [25(OH)D] serum levels and genetic variation related to vitamin D receptor (VDR) binding. The objective was to identify whether VDR binding variants (VDR-BVs) previously implicated in adult-onset MS were associated with pedMS using genetic instrumental variables (GIVs).

METHODS: Using previously identified VDR-BVs to construct individual GIVs with two-sample MR, we investigated associations with pedMS in …

Mechanisms Of Photoreceptor Protection Upon Targeting The Nrl-Nr2e3 Pathway, Daniel P Murphy, Alexander V Kolesnikov, Cynthia L Montana, Zaid M Khaja, Yu Liu, Vladimir J Kefalov, Joseph C Corbo

2020-Current year OA Pubs

Acute knockout of the rod photoreceptor transcription factor

Methods For Joint Modeling Of Longitudinal Omics Data And Time-To-Event Outcomes: Applications To Lysophosphatidylcholines In Connection To Aging And Mortality In The Long Life Family Study, Konstantin G Arbeev, Olivia Bagley, Svetlana V Ukraintseva, Alexander Kulminski, Eric Stallard, Michaela Schwaiger-Haber, Gary J Patti, Yian Gu, Anatoliy I Yashin, Michael A Province

2020-Current year OA Pubs

Studying the relationships between longitudinal changes in omics variables and event risks requires specific methodologies for joint analyses of longitudinal and time-to-event outcomes. We applied two such approaches (joint models [JM], stochastic process models [SPM]) to longitudinal metabolomics data from the Long Life Family Study, focusing on the understudied associations of longitudinal changes in lysophosphatidylcholines (LPCs) with mortality and aging-related outcomes. We analyzed 23 LPC species, with 5,066 measurements of each in 3,462 participants, 1,245 of whom died during follow-up. JM analyses found that higher levels of the majority of LPC species were associated with lower mortality risks, with the …