Digital Commons Network^™

Aldh7a1 Gene And Its Related Pyridoxine-Dependent Epilepsy, Flavia M.C. La Mendola, Tiziana Timpanaro, Daniela Caruso, Maria Teresa Garozzo, Santiago Presti, Catia Romano, Elena R. Praticò, Antonio Zanghì, Raffaele Falsaperla

Journal of Pediatric Neurology

Despite being classically reported as caused by mutations in solute carriers genes (SLC2A1), it has been recently shown that also mutations in ALDH7A1 can cause pyridoxine-dependent epilepsy (PDE). ALDH7A1 is a gene encoding for the antiquitin, an enzyme that catalyzes the nicotinamide adenine dinucleotide-dependent dehydrogenation of L-α-aminoadipic semialdehyde/L-Δ1-piperideine 6-carboxylate. It is a highly treatable disorder, but nevertheless it is still not certain when to consider this diagnosis and how to test for it. It is possible to identify a classical form and an atypical one of PDE associated with more than 70 mutations of ALDH7A1 gene. The typical form is …

Beyond The Lawn: Bylaws For Biodiversity - Evidence-Based Recommendations For Future Practice And Policy Development, Nina-Marie Lister, Sabrina Careri

Cities and the Environment (CATE)

Across Canada and globally, there is growing momentum to mitigate the impacts of climate change and habitat loss on biodiversity. These challenges transcend the jurisdiction of any single government or agency. In response, people across urban areas are increasingly taking action, particularly through landscaping strategies that foster and support biodiversity at home in their gardens. In North American cities, private lands make up a significant and often majority of the urban matrix. As such, private lands offer opportunities to address anthropogenic socio-environmental impacts in cities in ways that complement municipal initiatives on public lands. Specifically, rewilding initiatives on private lands, …

District Impact On State-Wide Charter School Policy: Has Normandy Schools Collaborative Punctuated The Equilibrium? Charter School Policy Analysis Of The Missouri Department Of Elementary And Secondary Education, Tracey L. Cannon

Dissertations

Abstract

Punctuated Equilibrium Theory, as applied by Niles Eldredge and Stephen Jay Gould (1941-2002) to the study of species evolution, examined the infrequency of morphological change or stasis discontinuity.1 While groundbreaking in the field of Paleontology and the study of fossil change, the theory has been well-fitting to the decision sciences as introduced by Baumgartner and Jones2, in their 1993 and 2010 publications. Recognizing typical, incremental policy change, its use in this research looks at how stasis, stagnant policy can be punctuated by changes in political or societal views. This study examines the impact of heightened problem discussion for agenda …

A Narrative Study Of How Secondary School Teachers Experience Generative Ai Writing Tools In The English Language Arts Classroom, Nick Brodell

Doctor of Education (EdD)

With the presence of artificial intelligence (AI) programs such as ChatGPT commonplace in secondary English language arts (ELA) classrooms, scholars and practitioners are both curious and skeptical about the technology’s ability to help students improve their ELA skills. Teachers have been using tutoring software and Web 2.0 technologies for decades, yet AI writing tools offer new functionalities. Teachers and administrators have experiences engaging with the benefits and burdens of the technology, and scholars of composition pedagogy, ITS (intelligent tutoring systems), and ML (machine learning) have theories about students using it for skills acquisition and practice. This study collected and analyzed …

Signal And Noise Analysis In An Undergraduate Instrumental Analysis Laboratory, Ken Overway

Chemistry Faculty Scholarship

Understanding the difference between signal and noise in a measurement is fundamental to analytical chemists. The typical instruments found in an undergraduate chemistry department can be used to teach this concept, as well as signal averaging, by giving students hands-on experience with measurements and data processing. This work presents an undergraduate laboratory module (homework and experiment) that provides specific instructions for using gas chromatographs, fluorometers, a nuclear magnetic resonance spectrometer, an atomic absorption spectrophotometer, and an infrared spectrometer to collect replicate measurements and a framework for calculating the noise level, baseline, signal level, signal-to-noise ratio (SNR), and the signal thresholds …

Cilia In The Brain Display Region-Dependent Oscillations Of Length And Orientation, Roudabeh Vakil Monfared, Sherif Abdelkarim, Pieter Derdeyn, Kiki Chen, Hanting Wu, Kenneth Leong, Tiffany Chang, Justine Lee, Sara Versales, Surya M. Nauli, Kevin Beier, Pierre Baldi, Amal Alachkar

Pharmacy Faculty Articles and Research

In this study, we conducted high-throughput spatiotemporal analysis of primary cilia length and orientation across 22 mouse brain regions. We developed automated image analysis algorithms, which enabled us to examine over 10 million individual cilia, generating the largest spatiotemporal atlas of cilia. We found that cilia length and orientation display substantial variations across different brain regions and exhibit fluctuations over a 24-h period, with region-specific peaks during light-dark phases. Our analysis revealed unique orientation patterns of cilia, suggesting that cilia orientation within the brain is not random but follows specific patterns. Using BioCycle, we identified rhythmic fluctuations in cilia length …

The Kentucky Microbiome Dataset, Rachel L. Washburn, Douglas C. Curl

Research Data--KGS

The Kentucky Microbiome Dataset is a continuously updated geolocated dataset which describes the distribution and diversity of microorganisms across the Commonwealth of Kentucky. Each geolocated point represents a location reported in the peer-reviewed literature where microorganisms were sampled from soil, water, geologic substrates, or clinical settings. Environmental studies include only non-host-associated, non-clinical microorganisms sampled from natural environments. Clinical studies are mapped separately to highlight locations where investigations into host-associated or human health-related microbes have occurred.

For each location, associated metadata include study information, taxonomic classifications, sample type, sampling method, latitude and longitude, sampling date, publication reference, and a direct link …

Online Detection Of Tomato Internal And External Quality Based On Iot And Machine Learning, Gao Yanfei, Xu Xuefeng, Huang Yu, Zhao Xiaofei

Food and Machinery

［Objective］ To address the low efficiency and strong subjectivity of traditional manual tomato grading,this study developed an online tomato internal and external quality detection and grading system based on the Internet of Things (IoT) and machine learning technologies,enabling real -time,non -destructive detection of both internal and external quality attributes.［Methods］ By integrating machine vision and near -infrared spectroscopy,and leveraging IoT and machine learning algorithms,a comprehensive system for online,non-destructive tomato detection and grading was designed and implemented.Real -time acquisition of external images and internal spectral information of tomatoes was performed.External defects,shape index,and diameter were detected using deep learning models,while soluble solids content …

Effect Of Polygonatum Sibiricum Powder On Properties Of Dough And Quality Of Bread, Wu Kunwei, Wei Ming, Qian Senhe, Li Ziman, Xie Yan

Food and Machinery

［Objective］ To explore the application of Polygonatum sibiricum powder in bread,enhancing its health benefits.［Methods］ Polygonatum powder is added to wheat flour to study its effects on starch gelatinization,gluten structure,dough rheological properties,and bread baking quality.［Results］］ The addition of Polygonatum powder increases the gelatinization temperature of the flour,while peak viscosity and retrogradation values decrease.The gluten yield in the dough is reduced.Changes in the secondary structure of gluten proteins are observed,with an increase in the content of random coil structures.Polygonatum sibiricum powder increases dough hardness,elasticity modulus,and viscosity modulus,but it reduces the dough's fermentation volume.The baking quality of the bread is affected,with the bread …

Antibiotic Use In Rural Kenyan Livestock: Navigating Misuse, Experience Gaps And Amr Risks, Lucy Obolensky, Esbon Wambugu, Edna Kubai, Iain Doig, Miriam Beattie, Michael Dillon

Peninsula Medical School

Antimicrobial resistance (AMR) is an escalating global health threat, with the greatest risk observed in low- to middle-income countries, particularly in the global south. The World Health Organization advocates for a One Health approach to address AMR, promoting collaboration across sectors, including in agriculture. This study aims to enhance understanding of antimicrobial use and stewardship in livestock within pastoralist communities in northern Kenya, where there is limited information. The study employed a qualitative approach, using semi-structured interviews to gather data on farming practices and antibiotic use. Interviews were conducted by trained volunteers proficient in Swahili and Ma (a Maasai language), …

Approximating Data-Driven Combinatorial Search In The Feature Space: Pwfs, Mehmet Bugrahan Ayanoglu

USF Tampa Graduate Theses and Dissertations

This dissertation presents the development and application of a novel feature selection method, Probability Weighted Feature Selection (PWFS), designed to address key challenges in machine learning involving high-dimensional, noisy, or biased datasets. It is a comprehensive exploration of feature selection strategies to enhance the performance, interpretability, and practicality of machine learning models in both engineering systems and educational data mining. PWFS introduces a structured, probabilistic approach to feature selection that adaptively weights features based on their empirical contribution to model performance. This method not only accelerates convergence to near-optimal feature subsets but also enhances flexibility for ensemble learning by encouraging …

Autism And Down Syndrome: An Examination Of The Baby And Infant Screen For Autism Traits In Toddlers With Down Syndrome, Celeste Tevis

LSU Doctoral Dissertations

Current research suggests that children with Down Syndrome (DS) are at an increased risk for autism spectrum disorder (ASD) compared to the general population. Detecting ASD early in children with DS is crucial to providing access to additional treatment services and supports that may be necessary. Despite a critical need to identify ASD as early as possible, diagnoses of ASD are often delayed in individuals with DS. It can be especially difficult for clinicians to differentiate between symptoms attributed to intellectual developmental disorder (IDD), a primary feature of DS, and possible ASD symptoms. Only two research studies have evaluated the …

Investigation Of Subcellular Damage And Long-Term Non-Cancerous Cardiovascular Effects Following Whole-Body Photon Irradiation, Nousha Afshari

LSU Doctoral Dissertations

Current radiobiological models focus primarily on nuclear DNA damage and fail to fully explain radiation-induced noncancerous health effects. This dissertation investigates whether non-nuclear subcellular structures contribute to radiation-induced biological damage through experimental biomarker analysis and computational microdosimetry. The central hypothesis posits that whole-body photon irradiation results in subcellular non-nuclear damage, specifically increased circulating mitochondrial DNA concentrations, that correlates with radiation dose and long-term noncancerous effects such as cardiovascular disease.

Specific Aim 1 measured mitochondrial and nuclear DNA concentrations in banked blood samples from 72 Chinese rhesus macaques that previously received whole-body photon irradiation ($0-10$ Gy) from Cobalt-60 or LINAC sources. …

Unveiling The Clinicopathological Outcomes Of Breast Cancer In Young Women: A Perspective From Resource-Limited Settings, Kulsoom Shaikh, Aiman Arif, Mehwish Mooghal, Lubna Vohra

Sultan Qaboos University Medical Journal

Objectives: This study aimed to assess the incidence of breast cancer in young women over a ten-year period and examine its association with clinical characteristics, risk factors, treatment modalities, and survival outcomes. Methods: A retrospective analysis of the Breast Cancer Registry at our institution was conducted. Of the 2,238 women diagnosed with breast cancer between 2012 and 2021, 535 (23.9%) were aged 40 years or younger at the time of diagnosis. Cases with missing data for independent variables were excluded from the respective analyses. Results: The mean age at diagnosis was 34.5 years (range: 15–40). The most common clinical …

Comparison Of Leveling A Deep Curve Of Spee With Extractions Versus Non-Extraction: A Biomechanical Perspective, Mitchell James Muller

Master's Theses (2009 -)

Introduction: This study evaluated and compared orthodontic forces from different archwire designs and materials used to correct a deep curve of Spee. Methods: A three-dimensional orthodontic force tester (OFT) was used to measure forces and moments exerted by archwires on brackets from central incisors to first molars. Two setups were tested: one with a full dentition from central incisor to first molar, and another simulating bicuspid extractions with the first premolar removed. Dentoform models representing a deep curve of Spee were created using cone-beam computed tomography (CBCT) data. The setup employed 0.022 × 0.028-inch zero-prescription brackets secured with elastomeric ligatures. …

Establishment Of A Reverse Genetics System For Studying Human Immune Functions In Mice, Priya Pal, Shuai Gao, Hongbo Gao, Xiwen Qin, Marina Cella, Qiankun Wang, Liang Shan

2020-Current year OA Pubs

Reverse genetics approaches in mice are widely used to understand gene functions and their aberrations in diseases. However, limitations exist in translating findings from animal models to human physiology. Humanized mice provide a powerful bridge to understanding human physiology and mechanisms of disease pathogenesis while maintaining the feasibility of working with small animals. Methods for generating humanized mouse models that allow scientists to probe contributions of particular genes have been rudimentary. Here, we established an efficient method for generating genetically modified human cord blood-derived CD34

Preparation And Properties Of Rutin-Casein Complexes, Zhang Nan, Liu Lili, Yang Le, Li Sisi, Zhang Xinyuan

Food and Machinery

［Objective］ To investigate the effect of complexes produced by protein -polyphenol interaction on the bioavailability of polyphenols and proteins.［Methods］ Rutin -casein complexes are prepared using rutin and casein as raw materials.Their physicochemical and structural properties are analyzed.［Results］］ The optimal preparation conditions for the rutin -casein complexes are determined as follows:mass ratio of rutin to casein =1.89∶1.00,reaction time of 5.94 h,pH 8.5,and temperature of 50 ℃.Under such conditions,the binding degree is 40.23%.Compared with casein,the complexes exhibit a decrease in emulsifying activity index (8.47 m2/g) and emulsifying stability (9.39%).The solubility increases by 5.11%,surface hydrophobicity is significantly enhanced,and antioxidant activity markedly improves (P<0.05).Scanning electron microscopy reveals that the rutin -casein complexes display a lamellar structure with smooth surfaces,which may contribute to its functional properties.Infrared spectroscopy demonstrates peak broadening and redshifts in the amide I and II bands,and fluorescence intensity (including synchronous fluorescence ) decreases due to fluorescence quenching.It indicates that rutin alters the secondary structure and conformational arrangement of casein.［Conclusion］ Compared with the uncomplexed casein,the rutin -casein complexes are enhanced in solubility,surface hydrophobicity,and antioxidant activity.引用格式：张楠，刘丽莉，杨乐，等.芦丁—酪蛋白复合物的制备及特性研究 ［J］.食品与机械，2025，41（7）：9-17.C itation:ZHANG Nan,LIU Lili,YANG Le,et al.Preparation and properties of rutin-casein complexes [J].Food & Machinery,2025,41(7):9-17.

Effects Of Electron Beam Irradiation On Carotenoids And Capsaicinoids In Pepper Oils Prepared By Different Plant Oils, Ouyang Mengyun, Li Wenge, Cao Sheng, Mo Mo, Zhou Yiji, Zhang Qiling, Xu Yuanfang

Food and Machinery

［Objective］ To investigate the irradiation stability of carotenoids and capsaicinoids in pepper oils prepared using different plant oils.［Methods］ With rapeseed oil (RO),soybean oil (SO),and their blended oil (RSO ) as materials,pepper oils RO -P,SO-P,and RSO -P were prepared,and subjected to high -energy electron beam irradiation (10 MeV ).The effects of different irradiation doses (4~12 kGy ) on the peroxide value (PV),acid value (AV),carotenoid content,and capsaicinoid content of the pepper oils were investigated.［Results］］ Electron beam irradiation promoted lipid oxidation in the pepper oils,resulting in increased PV and AV values,with SO -P exhibiting the highest degree of lipid oxidation.When the irradiation dose …

Occurrence Of Pesticides In Oregon Coastal Waters And Effects On The Intertidal Sea Anemone, Anthopleura Elegantissima, Gabriella Mae Bleil

Dissertations and Theses

Pesticides are used globally for a wide range of applications including agricultural, forestry, roadsides, freshwater systems, and personal use. While pesticides have ensured efficient crop production, they are frequently transported away from application sites and are ubiquitous in terrestrial and aquatic environments. Pesticides are often detected in watersheds and rivers globally, which can have unintentional toxic effects on non-target organisms by interfering with cellular processes, behavior, feeding, reproduction, and disrupting endocrine processes. The aggregating anemone, Anthopleura elegantissima, is an important species along the North American Pacific coast due to its symbiotic relationships that contribute to high productivity, and its …

Src/Fn1 Pathway Activation Drives Tumor Cell Cluster Formation And Metastasis In Lung Cancer: A Promising Therapeutic Target, Zujun Que, Zhichao Xi, Dan Qi, Rongchen Dai, Yang Li, Mengfan Liu, Bin Luo, Jiajun Liu, Pan Yu, Yun Yang, Erxi Wu, Hongxi Xu, Jianhui Tian

Children’s Nutrition Research Center Staff Publications

Lung cancer remains the leading cause of cancer-related death globally, with metastasis driven by circulating tumor cells (CTCs)-particularly clusters-being a major treatment challenge. Despite their critical role, the biological differences between single CTCs and CTC clusters remain unclear. Here, we comprehensively compared their behavioral, transcriptomic, and proteomic profiles in lung cancer models. Compared with single cells, CTC clusters present enhanced metastatic potential, greater survival in the bloodstream and increased resistance to microenvironment. Mechanistically, the Src/FN1 pathway is centrally activated in clusters, promoting intercellular cohesion and protecting against immune clearance and stress in circulation. Pharmacological inhibition of Src with the clinical …

Mir-302a/B/D-3p Differentially Expressed During Frontonasal Development Is Sensitive To Retinoic Acid Exposure, Chihiro Iwaya, Akiko Suzuki, Goo Jun, Junichi Iwata

Faculty, Staff and Student Publications

Any failure in frontonasal development can lead to malformations at the middle facial region, such as frontonasal dysplasia, midfacial clefts, and hyper/hypotelorism. Various environmental factors influence morphogenesis through epigenetic regulations, including the action of noncoding microRNAs (miRNAs). However, it remains unclear how miRNAs are involved in the frontonasal development. In our analysis of publicly available miRNA-seq and RNA-seq datasets, we found that miR-28a-5p, miR-302a-3p, miR-302b-3p, and miR-302d-3p were differentially expressed in the frontonasal process during embryonic days 10.5 to 13.5 (E10.5–E13.5) in mice. Overexpression of these miRNAs led to a suppression of cell proliferation in cultured mouse embryonic frontonasal mesenchymal …

Molecular Epidemiology And Clinical Characterization Of Carbapenemase-Producing Enterobacter Species From An International Cohort, Jianping Jiang, Lauren Komarow, Carol Hill, Angelique E Boutzoukas, Blake Hanson, Cesar A Arias, Robert A Bonomo, Scott Evans, Yohei Doi, Michael J Satlin, Gregory Weston, Eric Cober, Sandra Liliana Valderrama-Beltran, Soraya Salcedo Mendoza, Zhengyin Liu, Bettina C Fries, Paul Ananth Tambyah, Henry F Chambers, Vance G Fowler, David Van Duin, Barry N Kreiswirth, Liang Chen

Faculty, Staff and Student Publications

Background: Despite the global public health threat posed by carbapenem-resistant Enterobacter spp, clinical and molecular epidemiological studies on international isolates remain scarce. Historically, the taxonomy of Enterobacter has been challenging, limiting our understanding of the clinical characteristics and outcomes of carbapenemase-producing Enterobacter spp infections.

Methods: Hospitalized patients enrolled in the CRACKLE-2 study (ClinicalTrials.gov, NCT03646227) from 2016 to 2018 with cultures positive for carbapenemase-producing Enterobacter spp were included. Clinical and microbiologic data were collected from health records. Whole genome sequencing was performed, and the population structures of selected predominant clones were analyzed.

Results: We enrolled 136 hospitalized patients with carbapenemase-producing …

Analyses Of Morphological Effects Of Cryopreservation On Plasmodium Yoelii Sporozoites, Lauren J. Topley

USF Tampa Graduate Theses and Dissertations

Malaria is a vector-borne disease and is a major global public health threat caused by the genus Plasmodium. Of the five species that infected humans, Plasmodium vivax and Plasmodium falciparum contribute the highest rates of morbidity and mortality. Research efforts to develop antimalarial drugs and effective vaccines is imperative to the prevention of future cases. Plasmodium has three reproductive stages: sporogonic, liver, and blood. Sporozoites are injected into the human host from an infected female Anopheles mosquito, which travel to the liver to initiate liver stage malaria. Since few sporozoites are successful, the liver stage is a bottleneck of the …

Vitamin K And Women's Health: A Review, Sharifa Alblooshi

All Works

Vitamin K is a fat-soluble vitamin essential in various physiological functions such as blood coagulation, bone metabolism, cardiovascular health, glucose regulation, immune function, neuroprotection, and vascular health. In this narrative review, studies from databases including PubMed, Google Scholar, Scopus, and the institutional database of Zayed University were selected. The role of vitamin K in women's health, with a focus on osteoporosis, postmenopausal health, cardiovascular health, diabetes, cancer, kidney health, brain health, vascular health, and pregnancy were explored. The role of vitamin K in activating vitamin K-dependent proteins is essential for many of its functions. Vitamin K-dependent proteins such as osteocalcin …

Oral Dosing Of The Nucleoside Analog Obeldesivir Is Efficacious Against Rsv Infection In African Green Monkeys, Jared Pitts, J Lizbeth Reyes Zamora, Savrina Manhas, Thomas Aeschbacher, Josolyn Chan, Vincent Cutillas, Varsha Nair, Nicholas C Riola, Arya Vijjapurapu, Meghan S Vermillion, Stacey Eng, Christopher Richards, Dong Han, Jason K Perry, Subhra Chaudhuri, Szu-Wen Liu, Clarissa Martinez, Nadine Peinovich, Kai-Hui Sun, Arthur Cai, Ross Martin, Jasmine Moshiri, Charlotte Hedskog, Darius Babusis, Dustin S Siegel, Rao Kalla, Vasanthi Avadhanula, Pedro A Piedra, Kim Stobbelaar, Peter L Delputte, Caleb Marceau, Roberto Mateo, Evguenia Maiorova, Hongmei Mo, Raju Subramanian, Richard L Mackman, Tomas Cihlar, Simon P Fletcher, John P Bilello

Faculty, Staff and Students Publications

Respiratory syncytial virus (RSV) is a significant cause of morbidity and mortality in high-risk populations. Although prophylactic options are available, there are no effective oral therapeutics for RSV infection. Obeldesivir (ODV) is an orally bioavailable prodrug of the nucleoside analog GS-441524, which is converted intracellularly to its active nucleoside triphosphate and inhibits the RSV RNA polymerase. Here we report the potent antiviral activity of ODV against geographically and temporally diverse RSV A and B clinical isolates (EC50: 0.20–0.66 μM). Resistance selection studies with ODV and GS-441524 against RSV identify a single amino acid substitution, I777L, in the L polymerase with …

Investigation Of The Interaction Between Diethyl Phthalate And Ovalbumin Via Spectroscopic Techniques And Molecular Docking, Fu Chen, Huang Qun

Food and Machinery

［Objective］ To explore the interaction mechanism between diethyl phthalate (DEP ) and ovalbumin (OVA ) using multispectral analysis and molecular docking techniques.［Methods］ The Stern -Volmer equation is applied to study the fluorescence quenching mechanism of OVA by DEP,as well as to determine the binding constants and the number of binding sites between them.Thermodynamic parameters are used to identify the primary types of interaction forces.Based on Förster's non -radiative energy transfer theory,the energy transfer efficiency and binding distance between DEP and OVA are calculated.Molecular docking simulations are used to investigate the interaction forces between DEP and OVA.［Results］］ DEP can statically quench …

Ultrasound-Assisted Enzymatic Extraction And Structural Characterization Of Pectin From The Tender Leaves Of Abelia Macrotera, Mao Qiqi, Luo Yang, Wang Jianglin, Luo Jie, Fan Xiankang, Liu Chengguo, Zhou Hui

Food and Machinery

［Objective］ To clarify the physicochemical properties,microscopic structure,monosaccharide composition,relative molecular mass,and structural characteristics of pectin in the tender leaves of Abelia macrotera.［Methods］ Pectin from the tender leaves of Abelia macrotera was extracted by ultrasound -assisted cellulase method.The microscopic structure was observed under scanning electron microscope.The monosaccharide composition was determined by high performance liquid chromatography.The relative molecular mass was determined by polysaccharide hydrolysis derivatization.The possible structure was inferred by infrared spectroscopy.［Results］］ The yield of pectin from the tender leaves of Abelia macrotera is 23.38%,the esterification degree was 1.17%,and the galacturonic acid content was 72.16%.The pectin is in the form of flakes with …

Determination Of Vitamin B12 In Infant Formula Milk Powder By Cyanide-Free Degradation Coupled With Hydrophilic Interaction Liquid Chromatography With Fluorescence Detection, Li Liang, Deng Wenjuan, Huang Weiqian, Wu Junfa, Qin Tianfu

Food and Machinery

［Objective］ To establish a method for determining the total vitamin B12 content in infant formula milk powder based on the indirect detection of α-ribazole,a degradation product and nucleoside component of vitamin B12.［Methods］ After protein denaturation and sugar removal,the sample underwent acid hydrolysis and enzymatic dephosphorylation to release α-ribazole,which was then purified using a boronate affinity column.Quantitative analysis was performed using hydrophilic interaction liquid chromatography with fluorescence detection (HILIC -FLD ).［Results］］ Alpha -ribazole showed good linearity in the range of 1.00~10.0 μg/L,with a correlation coefficient (R²) of 0.999 1.The spiked recovery ranged from 72.5% to 78.3%,with relative standard deviations (RSDs ) …

Preparation And Performance Of Visual Active Film By Purple Yam, Deng Fuyuan, Xie Wei, Li Xin, Wen Yanqing, Zhu Guiting

Food and Machinery

［Objective］ This study aimed to develop a visual active film using the inherent anthocyanins in purple yam as a natural indicator and its rich content of starch,polysaccharides,and proteins as the film -forming matrix,providing an intuitive and effective new approach for quality control of fish products.［Methods］ Purple yam was processed by raw,steamed,and boiled treatments,then freeze -dried to obtain three types of purple yam powders,which were used to prepare visual active films corresponding to each treatment method.The effects of different citric acid additions on film properties were investigated.Based on comprehensive analysis of film characteristics,the optimal active film was selected and applied for …

Optimization Of Sulfur-Free Color Protection Process And Comparison Of Drying Techniques For Honeysuckle, Zhai Yumei, Tang Xiaojuan, Fu Xiao, Gong Min, Liu Yunguo

Food and Machinery

［Objective］ To develop green processing technology to inhibit the browning of fresh honeysuckle (Lonicera japonica Thunb ) and enhance its drying quality.［Methods］ With polyphenol oxidase relative enzyme activity and color difference values as evaluation indicators,the process of sulfur -free compound color -protecting agents (citric acid,D-sodium erythorbate,and DL-sodium malate ) is optimized through response surface methodology.Additionally,the effect of various drying methods,including vacuum freeze drying and microwave drying,on the quality after color protection is compared and analyzed.［Results］］ Citric acid demonstrates the best anti -browning effect.The process parameters are optimal when citric acid concentration is 0.105 mol/L,with D-sodium erythorbate concentration of 0.124 mol/L …