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The Characterization And Detection Of Human Associated Pathogens Within Aquatic Sediments In Florida, Athena Giorgiou Capetanakis

All HCAS Student Capstones, Theses, and Dissertations

Thalassogenic diseases, infectious diseases caused by wastewater pollution of marine environments, have been plaguing the world for generations. Every millimeter of raw wastewater may carry millions of potentially pathogenic microorganisms. Water monitoring techniques generally rely on the sole detection of fecal indicator bacteria, while overlooking the significance of microbial data, especially via aquatic sediment. It is known that aquatic sediment can provide the ideal environment for pathogenic microorganisms to survive in. Using next-generation sequencing methods, amplifying the V4 region of the 16S rRNA gene, this study aimed to achieve the following objectives: 1) characterize the microbiome of the sediment in …

Pcsk9–Dyslipidemia Interplay In Children And Adolescents With Type 1 Diabetes: A Potential Modulator Of Vasculopathy, Eman Ramadan

Pharmacy

BACKGROUND: Proprotein convertase subtilisin/kexin type-9 (PCSK9) has recently emerged as an important vasculopathy modulator. However, limited data exist on its level and expression in children and adolescents with type 1 diabetes (T1D). AIM: To assess serum PCSK9 and gene expression among children and adolescents with T1D and correlate them with glycemia, dyslipidemia, and microvascular complications. METHODS: Fifty children and adolescents with T1D were compared to 50 matched healthy controls. Serum PCSK9 enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR) gene expression, glycated-hemoglobin, fundus, urinary albumin-to-creatinine ratio (uACR) and fasting lipids were assessed with calculation of the estimated-glucose …

Developing Microelectrode Arrays As Multiplex Point-Of-Care Diagnostics, Yu-Chia Chang

Arts & Sciences Graduate Student Theses and Dissertations

Antibiotic resistance poses a significant global health challenge, particularly in urinary tract infections (UTIs), where 92% of cases exhibit resistance to at least one antibiotic, causing over 260,000 deaths annually. The emergence of antibiotic-resistant bacteria and growing awareness of the adverse effects of unnecessary antibiotic use highlight the urgent need for better UTI diagnostics. Current state-of-the-art methods are either expensive and time-consuming, or they lack specific details about the nature of the antibiotic and only determine inflammation. This hinders timely and effective treatment decisions regarding whether an antibiotic is actually necessary. Given this backdrop, there is a demand for a …

An Enzyme-Coupled Isotope Dilution Mass Spectrometry Assay For Non-Adjacent Dna Photoproducts As Intrinsic Probes For G-Quadruplexes In Vitro, Savannah Sharee Scruggs

Arts & Sciences Graduate Student Theses and Dissertations

G-quadruplexes are noncanonical secondary structures that form in guanine-rich nucleic acid sequences and are thought to be involved in the control of gene expression.1 Unfortunately, it has been difficult to prove that these structures exist in vivo because of their dynamic nature. Rather than try to develop G-quadruplex specific binders as others have done,2 our idea is to photochemically trap certain types of G-quadruplexes by irreversible photoproduct formation3–5 and then detect the stable G-quadruplex-specific photoproducts by Isotopic Dilution Mass Spectrometry (IDMS).6 This IDMS method could then be used to unambiguously confirm the presence of specific types of G-quadruplexes in vivo …

Motivational Factors Affecting Female Students' Choice Of Stem And Non-Stem Majors At Us Universities, Buthaina Ruyyashi

Walden Dissertations and Doctoral Studies

The problem addressed in this study is the persistently low enrollment of female undergraduate students in science, technology, engineering, and mathematics (STEM) majors within U.S. universities. The purpose of this quantitative study was to examine the relationship between female undergraduate students' choice of STEM versus non-STEM majors and their motivational factors, as measured by the Motivations Influencing Course Choice (MICC) survey. The study was grounded in social cognitive career theory, which emphasizes the role of self-efficacy, outcome expectations, and personal goals in academic decision-making. The analysis assessed the predictive power of career aspiration, interest, helping, and loafing in determining major …

A Toolkit For Treating Obesity In Children With Metabolic Syndrome, Karen Mayers-Scott

Walden Dissertations and Doctoral Studies

This doctoral project seeks to bridge the existing knowledge gap in using Metformin to manage childhood obesity in patients with metabolic syndrome. The primary goal was to develop a complete toolkit to educate healthcare providers on utilizing Metformin in obese children with metabolic syndrome. Metabolic syndrome, characterized by higher triglycerides, decreased high-density lipoprotein cholesterol, increased blood pressure, elevated fasting plasma glucose, and increased waist circumference, offers considerable health hazards for children. The practice-focused question is, Is the toolkit deemed useful to providers for treating obesity in children with metabolic syndrome using metformin?” The project employed the theory of planned behavior, …

Gender In Translation: How The Contemporary Bible Is Challenging Androcentric Language In Translation Through Gender-Accurate And Feminist Hermeneutical Lenses, Matthew T. Gennard

Masters Theses

This Thesis surveys the changing practice of gender-accurate translation from the late nineteenth century to the present, with special consideration for the three key principles of gender-accurate translation: Generic ‘he,’ androcentric lexemes, and pronoun substitution. These focus areas are derived from an analysis of the five translation guidelines produced during the late twentieth-century gender-neutral controversy. The loss of individuality when introducing pronominal interchange or the masculine representative hermeneutical lens has left scholars divided on gender-accurate practices. This study surveys the sociohistorical development of the feminist hermeneutical lens and the changing linguistic trends of contemporary language, which challenge androcentric assumptions in …

Dosimetric Comparison Of Organ-At-Risk Constraints Across Multi-Resolution Levels In Eclipse Planning Optimization, Ji Hyun Jeon

Masters Projects

Background: In inverse treatment planning, the timing of applying organ-at-risk (OAR) constraints during the optimization process can influence dosimetric outcomes. This study investigates the effects of introducing OAR constraints at different stages of the multi-resolution optimization process within the Eclipse Treatment Planning System (TPS).

Purpose: To determine the optimal resolution level for applying OAR constraints that achieves the best dosimetric balance between target coverage and normal tissue sparing.

Methods: Thirteen prostate cancer treatment plans were generated using an anonymized pelvic CT dataset from The Cancer Imaging Archive. All plans were developed in GVSU Virtual Eclipse TPS using the Photon Optimizer …

Radioactive Contamination And Climate Warming Affect Physiological Performance Of Chornobyl Barn Swallows, Zbyszek Boratyríski, Timothy Mousseau, Anders Pape Møller

Faculty Publications

Global warming and degradation of natural habitats are the two main factors causing ecophysiological stress on individuals and risk for biodiversity. Hyperthermia is a common response to stress in homeothermic animals, in particular to heat, pathogens and environmental contamination. Resilience of biological systems to global warming may be deteriorated in polluted habitats. Here we investigated how body temperature of a wild bird, the barn swallow (Hirundo rustica), responded to global warming while simultaneously exposed to radioactive contamination from the Chernobyl accident. Our results showed that both high air temperatures (t = 15.55, df = 335, p <  0.0001) and elevated environmental radioactive contamination (t = 5.18, df = 8.09, p = 0.0008) increased internal body temperature of individuals. The additive effect suggests that birds might suffer hyperthermia in locally contaminated habitat (1.47% body temperature increase) while simultaneously exposed to globally rising temperatures (1.95% body temperature increase), potentially reducing the fitness of individual and the maintenance of breeding colonies. The cumulative and interactive negative effects of multiple stressors, such as those emerging from increasing habitat degradation and climate change, will likely contribute to biodiversity losses globally.

Mri-Based Radiomics For Outcome Stratification In Pediatric Osteosarcoma, Esther Ngan, Dolores Mullikin, Ashok J Theruvath, Ananth V Annapragada, Ketan B Ghaghada, Andras A Heczey, Zbigniew A Starosolski

Faculty, Staff and Students Publications

Background/Objectives: Osteosarcoma (OS) is the most common malignant bone tumor in children and adolescents; the survival rate is as low as 24%. Accurate prediction of clinical outcomes remains a challenge due to tumor heterogeneity and the complexity of pediatric cases. This study aims to improve predictions of progressive disease, therapy response, relapse, and survival in pediatric OS using MRI-based radiomics and machine learning methods. \

Methods: Pre-treatment contrast-enhanced coronal T1-weighted MR scans were collected from 63 pediatric OS patients, with an additional nine external cases used for validation. Three strategies were considered for target region segmentation (whole-tumor, tumor …

Hand Hygiene Knowledge, Attitudes, Practices, And Hand Dirtiness Of Primary School Students Before And After A Behavioral Change Intervention During The Covid-19 Pandemic, Belize 2022-2023, Anh N Ly, Christina Craig, Kelsey Mcdavid, Dian Maheia, Yolanda Gongora, Francis Morey, Russell Manzanero, Alexandra Medley, Allison Stewart, Allison Lino, Ramiro Quezada, Rosalva Blanco, Vickie Romero, Gerhaldine Morazan, Ella Hawes, Oluwadara Okeremi, Kanako Ishida, Matthew Lozier, Kristy O Murray

Faculty, Staff and Students Publications

Hand hygiene (HH) can prevent the spread of infectious diseases and school absenteeism. However, limited data exist on HH practices at schools. Our study assesses the impact of a pilot HH intervention in 12 schools in Belize during the coronavirus disease 2019 (COVID-19) pandemic. After a national assessment of existing water, sanitation, and hygiene resources (December 2021-January 2022), 12 pilot schools were selected to evaluate an HH intervention, which included environmental nudges and HH education. Baseline assessments occurred in March 2022, the HH intervention was implemented during October 2022-May 2023, and follow-up assessments were conducted in June 2023. Student knowledge, …

Msw Degree Completion: Foster Care Alumni’S Perceptions Of Barriers, Resilience, And Success, Christa Martinez

Walden Dissertations and Doctoral Studies

This capstone research project involved examining foster care alumni who successfully graduated from Master of Social Work (MSW) programs as full-time students. The purpose of this study was to address resilience factors and this population’s perceptions of barriers, resilience, and success in terms of completing full-time MSW programs and career outcomes. The resiliency theory and a strength-based-perspective were frameworks for this research. This basic qualitative research study involved using purposive sampling, and 11 foster care alumni were interviewed who graduated from full-time MSW graduate programs in the United States. Through thematic analysis, themes emerged were lack of economic resources impacting …

The Association Between Unemployment, Income, Education, Healthcare Access, Mental Health And High-Risk Hiv Behavior In The United States, Tia Smith

Walden Dissertations and Doctoral Studies

High-risk HIV behavior remains a critical public health concern in the United States, particularly among socioeconomically and psychologically vulnerable populations. Despite extensive prevention efforts, a gap persists in understanding how structural and mental health factors jointly contribute to HIV risk. This study addresses that gap by examining the association between socioeconomic status (SES), including income, education, employment, and healthcare access, and mental health status (diagnosis of depression and days of poor mental health) on high-risk HIV behavior at the individual level. Guided by the social determinants of health (SDOH) theoretical framework, the study utilized secondary data for over 40,000 adults …

Updates In Contemporary Management Of Singleton Pregnancies Complicated By A Short Cervix, Moti Gulersen, Vincenzo Berghella, Eran Bornstein

Department of Obstetrics and Gynecology Faculty Papers

Singleton pregnancies complicated by a short cervical length (≤25 mm) are at significantly increased risk for spontaneous preterm birth. Several treatment strategies aimed at reducing this risk and improving perinatal outcomes have been evaluated, including vaginal progesterone, cervical cerclage, and cervical pessary. This review summarizes the latest evidence regarding the efficacy of these interventions. Vaginal progesterone and/or cervical cerclage have been identified as proven evidence-based practices for preterm birth prevention and improve neonatal outcomes. Vaginal progesterone reduces the risk of preterm birth < 35 weeks by 27% (relative risk 0.73, 95% confidence interval 0.58-0.90). Cervical cerclage has been shown to reduce the risk of preterm birth < 35 weeks by 30% (relative risk 0.70, 95% confidence interval 0.55-0.89) in patients with a short cervical length and prior preterm birth. In contrast, recent data suggest that cervical pessary should no longer be considered a management option for these patients. A continued focus on individualized, evidence-based approaches remains essential to optimizing outcomes in this high-risk population.

Emergence Of Norms In The Presence Of Social Network Structure In A Stochastic Evolutionary Game Theory Model, Parker Foresman

Dissertations and Theses

In their 2000 paper, The Emergence of Classes in a Multi-Agent Bargaining Model, Axtell, Epstein, and Young introduced an agent-based bargaining model to study how social norms can emerge and persist from the decentralized, asynchronous interactions of individual agents in the presence of noise, even when these agents have no predefined preferences or advantages. This thesis extends their model by incorporating structured interaction networks, allowing us to investigate how the topology of agent interactions influences long-run dynamics and norm formation. Using a custom simulation package built in the Julia programming language, we replicate the original results under a complete …

Dynamic Mutational Profiling Of Binding Interactions And Allosteric Networks In Conformational Ensembles Of The Sars-Cov-2 Spike Protein Complexes With Classes Of Antibodies Targeting Cryptic Binding Sites: Confluence Of Binding And Allostery Determines Molecular Mechanisms And Hotspots Of Immune Escape, Mohammed Alshahrani, Vedant Parikh, Brandon Foley, Gennady M. Verkhivker

Mathematics, Physics, and Computer Science Faculty Articles and Research

The ongoing evolution of SARS-CoV-2 variants has underscored the need to understand not only the structural basis of antibody recognition but also the dynamic and allosteric mechanisms that could underlie complexity of broad and escape-resistant neutralization. In this study, we employed a multi-scale approach integrating structural analysis, hierarchical molecular simulations, mutational scanning and network-based allosteric modeling to dissect how Class 4 antibodies (represented by S2X35, 25F9, and SA55) and Class 5 antibodies (represented by S2H97, WRAIR-2063 and WRAIR-2134) can modulate conformational behavior, binding energetics, allosteric interactions and immune escape patterns of the SARS-CoV-2 spike protein. Using hierarchical simulations of the …

Biomarkers In Renal Cell Carcinoma: A Systematic Review And Immunohistochemical Validation Study, Brett Berezowski, Robert Boothe, Billy Chaplin, Sharon J Del Vecchio, Zakariya Fares, Tyrone L R Humphries, Keng Lim Ng, Taylor Noonan, Hemamali Samaratunga, Aaron Urquhart, David A Vesey, Simon T Wood, Glenda C Gobe, Robert J Ellis

Faculty, Staff and Student Publications

The worldwide incidence of renal cell carcinoma (RCC) rose by 22% between 2012 and 2022. In Australia, RCC accounted for 2.8% of all cancer diagnoses and contributing to 1.8% of cancer-related deaths. Identification of RCC biomarkers may aid in diagnosis and management. Methods: A systematic review of immunohistochemical markers of RCC studies published between 1990 and 2019 was undertaken to select candidate biomarkers of RCC. Immunohistochemical staining of 73 clear cell RCC tumors and paired normal tissue was undertaken using selected markers. Semi-quantitative and quantitative analysis of staining intensity between paired samples was undertaken to evaluate utility as potential …

Immunomodulatory Effects Of Gold Nanoparticles: Impacts On Immune Cells And Mechanisms Of Action, Khadijeh Koushki, Prapannajeet Biswal, Geraldine Vidhya Vijay, Mahvash Sadeghi, Sajad Dehnavi, Ngoc Tuyet Tra, Sai Kumar Samala, Mahdieh Yousefi Taba, Arjun Balaji Vasan, Emily Han, Yuri Mackeyev, Sunil Krishnan

Faculty, Staff and Student Publications

Traditional anti-inflammatory medications-such as corticosteroids, biological agents, and non-steroidal anti-inflammatory drugs-are commonly employed to mitigate inflammation, despite their potential for debilitating side effects. There is a growing need for alternative next-generation therapies for symptomatic, unchecked, and/or detrimental inflammation with more favorable adverse effect profiles. The long history of use of gold salts as anti-inflammatory agents and the more recent exploration of gold nanoparticle (AuNP) formulations for clinical indications suggest that the targeted delivery of nanoparticles to inflammatory sites may be a promising approach worth investigating. Coupled with peptides that specifically target immune cells, AuNPs could potently counteract inflammation. Here, we …

Nutrition Anthropology In Global Malnutrition In Children Under Five, Katherine Michelle Dennison Owens

Doctoral Dissertations and Projects

Food is essential to growth and development for people and nations. Too little or too much food can lead to undernutrition or overweight, both forms of malnutrition. While there have been improvements in undernutrition, overweight is increasing, causing the double burden of malnutrition globally, which is especially true in low-income and middle-income countries. Understanding nutrition anthropology's economic, environmental, and socio-cultural factors is critical to combat malnutrition's burden in different populations. Shaping policies and investments in malnutrition need to be strategic to have a measurable impact on malnutrition improvements. This study identified nutrition anthropology factors using six economic, environmental, and socio-cultural …

Sport Specific Postural Deviations: An Analysis Of Influence, Intervention, And Long Term Postural Health In Athletes, Thomas J. Mcguire Jr.

Doctoral Dissertations and Projects

This dissertation investigated sport-specific postural deviations in college-aged individuals aged 18–21 by comparing those who participated in a high school sport for at least four consecutive years with their non-athletic peers. The study aimed to identify prevalent postural deviations and explore their implications for long-term musculoskeletal health. Drawing from biomechanical and ergonomic literature, the study employed a quasi-experimental static-group comparison design to evaluate 60 participants (30 athletes, 30 non-athletes) using the PostureScreen mobile app. Postural deviations were measured in both the frontal and sagittal planes across four regions: head, shoulders, pelvis, and knees. Independent samples t-tests were conducted to examine …

A Study Of The Impact Of The Impostor Syndrome On Women Of Color In Leadership Roles In Predominantly White Institutions, Alecia M. Ho-Sang

Doctoral Dissertations and Projects

This qualitative phenomenological study explored the emotional, spiritual, and physical ramifications of impostor syndrome on women of color in leadership roles within predominantly white institutions using social cognitive theory. The impostor syndrome was coined in 1978 by psychologists Suzanne Inmes and Pauline Rose Clance (Clance & Imes, 1978, p. 241). It is the experience of individuals who, despite possessing the qualifications for esteemed positions, attribute their achievements to sheer luck. Benefitting from diverse social and organizational theories, including achievement goal theory and social identity theory, this study draws on social cognitive theory. It illustrates the multifaceted impact of environmental, behavioral, …

Vulnerable Student Populations’ Understanding Of Title Ix, Campus Support, And Supportive Resources: A Hermeneutical Phenomenological Study, April Lynn Wright

Doctoral Dissertations and Projects

This hermeneutical phenomenological study examined how vulnerable student populations on college campuses perceived Title IX, campus support, and campus resources. The theory guiding this study is Homans’s social exchange theory, which connects the three pillars of rules, resources, and social relationships. The connection between the theory and the study is that while Title IX has guidelines established to support students after a campus sexual assault, the guidelines do not mean much if a student who has experienced a campus sexual assault is unsure of the support and resources that are available to them. While social exchange theory highlighted the rules, …

Faculty Experiences With Evaluation Practices Within A Community College, Janie Dobbins Hamrick

Doctoral Dissertations and Projects

The purpose of this phenomenological study is to understand faculty experiences with evaluation practices for teachers within two-year higher education institutions in the Southeast region of the United States. The theory guiding this study is transformational leadership theory as it aids in understanding the problem that some faculty experience with evaluation practices established by ineffective leadership. By researching transformational leadership theory, researchers have determined that leadership style influences faculty experiences with teacher assessment procedures like evaluation and observation practices. The Central Research Question is, how are teaching evaluation experiences of community college faculty influenced by perceived supervisor leadership style? The …

The Exploration Of Self-Management Support Programs For African American Adults Ages 18 To 75 In Harnett County, North Carolina, Moya Camille Henny

Doctoral Dissertations and Projects

This phenomenological study explored the health needs and self-management experiences of African American adults ages 18 to 75 living with type 2 diabetes and hypertension in Harnett County, North Carolina. The study aimed to understand how self-management support programs could be better tailored to meet the cultural, social, and behavioral needs of this population, who face a disproportionate burden of these chronic conditions. Guided by Critical Care Theory, Chronic Care Model, and Social Cognitive Theory, the research examined how systemic inequalities, community resources, and personal beliefs shaped participants’ ability to manage their health. Data were collected through in-depth, semi-structured interviews …

A Phenomenological Study Of The Lived Experiences Of Teachers From The Northeast Region Of The United States And How They Cope With Workplace Stress And Burnout, Zakkiyyah W. Sally

Doctoral Dissertations and Projects

The purpose of this qualitative phenomenological study was to better understand the lived experiences of Pre-K to 12th grade teachers from the Northeast Region of the United States as it relates to the development of burnout and stress. The central phenomenon of the study has been to discover ways teachers choose to cope and best manage stress, which can lead to burnout. This research study also served to investigate school related resources available to teachers to help support them with stress, burnout, and coping. Teachers have noted that they manage stress and burnout with various strategies, such as mindfulness, compassion, …

Single-Working Parents’ Psychological Autonomy And Psychological Well-Being And The Mediating Role Of Work-Life Balance, Patrina Anastasia Lowrie

Doctoral Dissertations and Projects

Single-working parents (SWPs) shoulder dual roles and responsibilities, often resulting in strained psychological autonomy (PA), psychological well-being (PWB), and work-life balance (WLB) across their work and nonwork domains. These overlapping responsibilities create challenges in sustaining a quality of life over time. The specific problem this study addressed is that many SWPs in leadership positions do not manage their emotional center well, and the relationships among their PA, PWB, and WLB remain unknown. The purpose of this quantitative correlational study was to examine whether WLB mediates the relationship between PA and PWB in adult SWPs. Research questions were developed to explore …

Formerly Incarcerated Women, Adverse Childhood Experiences, And Resilience: A Qualitative Descriptive Study, Patricia Celina Simms

Doctoral Dissertations and Projects

The purpose of this qualitative descriptive case study was to explore how formerly incarcerated females who have experienced adverse childhood experiences (ACEs) perceived their participation in rehabilitation programs while incarcerated and its influence on their resilience and reintegration into society in Virginia. This study is based on the Community Resiliency Model (CRM) by Miller-Karas (2015), which explains how individuals and communities manage trauma and build resilience. The CRM emphasizes self-regulation, social connections, and internal resource development, reflecting participants’ transformative journeys. The model suggests that structured programs in supportive communities aid emotional stabilization and long-term resilience. Participants did not mention the …

Childhood Emotional Maltreatment: A Phenomenological Approach Of Its Influence On Adult Attachment And Romantic Relationships, Patricia A. Smetana

Doctoral Dissertations and Projects

This phenomenological qualitative study examined the lasting effects, if any, of childhood emotional maltreatment (CEM) on adult females' romantic relationships. The research explored participants’ perceptions of relationship fundamentals that influence adult attachment formation and the quality of romantic relationships, emphasizing the crucial role of adult attachment in relational quality and overall well-being. Online, two-part semi-structured interviews were conducted with each participant, yielding a total of 22 interviews. These interviews investigated the participants’ perceptions of how CEM shaped their views of their own and others’ behaviors within adult romantic relationships. The study focused exclusively on adult female survivors of CEM, excluding …

The Title Of My Project: Screen Time And Children's Health, Elisabeth Hernandez

Doctoral Dissertations and Projects

Excessive screen time is harmfully affecting families emotionally, physically, and mentally (Brindova et al., 2014). Eating habits, physical exercise, sufficient sleep each night, and communication with family and peers are increasingly declining as screen time is increasing (Siegle, 2017). With the rise in screen time, young people are spending more time alone (Gingold et al., 2014). Behrens et al. (2019) “explored the cause of anxiety and depression in children as it relates to the use of technology” (p. 474). Since the increase in technological advances, there has also been an increase in cyberbullying. “Setting limits on the amount of time …

Development Of A Physiologically Based Pharmacokinetic (Pbpk) Model And Evaluation Of Antioxidant Capacity For Polypodium Leucotomos Extract, Christopher L. Basel

Doctoral Dissertations and Projects

The purpose of this study was to develop an equation to calculate the approximate concentration of active components in orally administered Polypodium leucotomos fern extract (PLE) and to develop physiologically based pharmacokinetic (PBPK) models for an active component of PLE or its metabolite to address the need for human pharmacokinetics (PK). Model differences based on ethnic groups were also investigated. This was a quantitative study using secondary data. It has been estimated that skin cancers account for approximately 40% of all human cancers and that approximately 13,000 people will die of skin cancer in 2024. People with darker skin experience …