Digital Commons Network^™

Histochemical Demonstrations Of The Lens Of The Sailfin Molly (Poecilia Latipinna) With Retinal Adaptation, Shaimaa Awad Abid, Mukhtar Khamis Haba

Baghdad Science Journal

The study revealed that histochemical examination of the lens and retina in the fish Poecilia latipinna reflects adaptation and acclimation to dark and light conditions. The tissue sections of the lens and retina were stained with different stains and examined by a light microscope. The lens was found to be spherical in shape, transparent, hard, and avascular. The lens was surrounded by a cellular homogeneous capsule and followed by cuboidal epithelial cell layer, which was situated beneath the capsule. The epithelial cells elongated into lens fibers that were arranged in concentric rings, and the lens fiber membranes took a serrated …

Synthesis, Biological Activity And Molecular Docking Study Of Some New Chalcones, 5,6-Dihydropyrimidin-2-Ol And 5,6-Dihydropyrimidin-2-Thiol Derivatives Bearing 1,2,3- Triazoline, Hayder Raheem Ali, Ahmed Wahed Naser

Baghdad Science Journal

Many new chemicals, including chalcones, derivatives of 5,6-dihydropyrimidin-2-ol and 5,6-dihydropyrimidin-2-thiol, are synthesized in this work. The starting materials are substituted acetophenone derivatives involving 1,2,3-triazoline (1). Chalcone derivatives (2a-h) were obtained by condensing compound (1) with some substituted aromatic aldehydes in the presence of 40% potassium hydroxide KOH. The process of producing pyrimidin-2-ol derivatives (3a–e) by cyclizing produced chalcone derivatives (2a–e) urea in the presence of sodium hydroxide. Equivalent pyrimidin-2-thiol derivatives (4a-d) were produced when chalcone derivatives (2a-d) reacted with thiourea in the presence of sodium hydroxide. The …

Evaluating The Selectivity Of Novel Zic-Hilic Columns And An Application For Determining Antihistaminic Drugs In Pharmaceutical Preparations, Ashraf Saad Rasheed, Mustafa J. Mohammed

Baghdad Science Journal

The hydrophilic interaction liquid chromatography (HILIC) conjugated with UV spectrometry was employed to the determination of two antihistaminic drugs (Acrivastine and fexofenadine) in pharmaceutical formulations using two lab-made zwitterionic HILIC stationary phases (ZIC-2 and ZIC-5). The study aimed to investigate the impact of separation parameters including acetonitrile (ACN) content, pH of the eluent, and acetate buffer concentration on the retention behavior of Acrivastine and fexofenadine. A mixture of 3.0 mM acetate buffer (pH 4.75) and ACN (30:70) was used as eluent, with a flow rate of 0.6 mL/min. The detection wavelength was controlled at 267nm. The linearity of Acrivastine and …

Quantification Of Bioactive Compounds In Coffea Arabica And Their Impact With Omega-3 Supplementation On Non-High-Density Lipoprotein Levels In Hyperlipidemia-Induced Rats, Sojoud M. Al-Zoubi, Shady H. Awwad, Reem A. Issa, Ahmad Q. Daraosheh, Talal Al-Qaisi, Husni Farah, Khaled W. Omari, Beisan A. Mohammad, Mumen Amer, Rula F. Khuzaie, Mahmoud S. Abu-Samak

Baghdad Science Journal

This study aims to evaluate the levels of coffee lipids, diterpenes, chlorogenic acid (CGA), and caffeine in coffee samples with different roasting levels. Additionally, it aims to assess the impact of coffee extracts with/without omega-3 supplementation on lipid profile parameters. Liquid-liquid and soxhlet extraction, and quantitation were performed using HPLC-DAD. Two groups of Wistar rats were used: non-hyperlipidemic and hyperlipidemic. Blood samples were collected before and after hyperlipidemia induction after six weeks. The lipid profile: total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglyceride (TG), non-high-density lipoprotein (non-HDL), and total cholesterol-to-high-density lipoprotein ratio (TC/HDL) were determined. A positive correlation …

Synthesis, Characterization, And Anticancer Evaluation Of Novel Nickel(Ii) And Copper(Ii) Complexes Derived From Isatin Thiosemicarbazones, Shahnaz Abdulhamid Hamad

Baghdad Science Journal

The complex of (2,2'-(7,7'-dimethyl-2,2'-dioxo-1,1',2,2'-tetrahydro-3H,3'H-5,5'-biindole-3,3'-diylidene) dihydrazinecarbo thioamide), denoted as L, was prepared by the reaction of o-tolidine with chloral hydrate and hydroxylamine hydrochloride in the presence of sodium sulfate and a co-solvent, absolute ethanol. The novel complexes of Ni(II) and Cu(II) with the ligand, L, have been synthesized from a reaction of one mole of L with two moles of NiCl₂ and CuCl₂ to produce complexes with the general formula [M₂LCl₂] [where M= Ni(II) or Cu(II)]. All three complexes are novel and have a square planar geometry. The metal ions in the two metal complexes …

Sox2 Regulates Foregut Squamous Epithelial Homeostasis And Is Lost During Barrett's Esophagus Development, Ramon U. Jin, Yuanwei Xu, T. Mamie Lih, Yang-Zhe Huang, Toni M. Nittolo, Blake E. Sells, Olivia M. Dres, Jean S. Wang, Qing K. Li, Hui Zhang, Jason C. Mills

2020-Current year OA Pubs

Esophageal adenocarcinoma is increasingly prevalent and is thought to arise from Barrett's esophagus (BE), a metaplastic condition in which chronic acid and bile reflux transforms the esophageal squamous epithelium into a gastric-intestinal glandular mucosa. The molecular determinants driving this metaplasia are poorly understood. We developed a human BE organoid biobank that recapitulates BE's molecular heterogeneity. Bulk and single-cell transcriptomics, supported by patient tissue analysis, revealed that BE differentiation reflects a balance between SOX2 (foregut/esophageal) and CDX2 (hindgut/intestinal) transcription factors. Using squamous-specific inducible Sox2-KO (Krt5CreER/+ Sox2Δ/Δ ROSA26tdTomato/+) mice, we observed increased basal proliferation, reduced squamous differentiation, and expanded metaplastic glands at …

Highly Potent And Subtype-Selective Sperm-Specific Potassium Channel Slo3 Inhibitors Display High Tissue Exposure In The Murine Female Reproductive Tract, Kayla J Temple, Ping Li, Maximilian D Lyon, Shweta Bhagwat, Leila Asadi, Sophia Li, Ronald Mccarthy, Celia M Santi, Et Al.

2020-Current year OA Pubs

To date, the study of drug exposure and distribution throughout the female reproductive tract has often been overlooked and neglected. Herein, we report the first highly potent and subtype-selective sperm-specific potassium channel 3 (SLO3) inhibitors as a new modality of contraceptives. After extensive

Informatics At The Frontier Of Cancer Research, Kathleen Noller, Malachi Griffith, Daniel Marcus, Et Al.

2020-Current year OA Pubs

Digitized healthcare data, high-throughput profiling technologies, and data repositories have facilitated the emergence of a new era of cancer research. Each data stream requires specialized analysis methods for interpretation. The data-driven era of cancer research requires the development, enhancement, and sustainment of informatics technology software infrastructure, including fundamental methodology development in artificial intelligence and data science. We review current and emerging informatics technology developments for cancer research and discovery, spanning molecular and cellular characterizations, image analysis, informatics, and therapeutics. Summarizing the diverse methods and applications of informatics throughout cancer research identifies themes and emerging areas for the next generation of …

Integration Of Spatial Protein Imaging And Transcriptomics In The Human Kidney Tracks The Regenerative Potential Of Proximal Tubules, Mahla Asghari, Sanjay Jain, Et Al.

2020-Current year OA Pubs

The organizational principles of nephronal segments are based on anatomical and physiological attributes that are linked to the homeostatic functions of the kidney. Recent molecular approaches have uncovered layers of deeper signatures and states in tubular cells that arise at various time points on the disease trajectory. Here, we introduce an analytical pipeline of multiplexed spatial protein imaging integrated with RNA expression to characterize proximal tubular subpopulations and neighborhoods in human kidney tissue. We demonstrate that, in reference tissue, a large proportion of S1 proximal tubular epithelial cells expresses thymus antigen 1 (THY1), a mesenchymal stromal and stem cell marker …

Ophthalmology's Role In The Academic Medical Center: A Perspective, Stephen D. Mcleod

Journal of Academic Ophthalmology

NA

A Novel 3d-Printed Silicone Simulation Model For Effective Instruction Of Marginal Eyelid Laceration Repair, Nicole Lifson, David Booy, Spandana N. Jarmale, Joseph Crozier, John Nguyen, Bradley Thuro, Albert S. Woo, Jamie Lea Schaefer, Michael E. Migliori

Journal of Academic Ophthalmology

Purpose We performed a prospective study to determine the efficacy of a novel silicone eyelid model in helping train residents to repair marginal eyelid lacerations. Methods 3D design software was used to create a scaffold for a silicone eyelid mold. A total of 18 ophthalmology residents participated in a training session on marginal eyelid laceration repair, practicing on these models. Prior to and following the session, residents anonymously responded to a survey assessing their degree of confidence in repairing marginal and non-marginal eyelid lacerations, as well as their overall opinion of the workshop. Questions related to their confidence and ability …

David Finfrock Diary Entry, David Finfrock

David Finfrock Antartica Exploration

David Finfrock's diary from his trip to Antarctica in December 2002.

Ai-Driven Solutions For Electricity Fraud Detection: A Data-Centric Approach, Fatemeh Alimoradi, Zahra Alimoradi, S. Mohammadali Zanjani, Ghazanfar Shahgholian

NJF Intelligent Engineering Journal

Electricity fraud detection presents a significant challenge for power distribution companies, as non-technical losses resulting from fraudulent activities adversely affect revenue and operational efficiency. This study presents a machine learning-driven approach for accurately identifying fraudulent electricity consumption patterns. A comprehensive analysis of electricity usage data is conducted using multiple classification models, including Support Vector Machine (SVM), K-Nearest Neighbors (KNN), a Stacking Model integrating SVM, KNN, Random Forest, and Gradient Boosting, as well as a Weighted Model leveraging confidence-based prediction adjustments. Model performance is evaluated using key metrics, including accuracy, precision, recall, F1-score, AUC-ROC, and confusion matrices. The results indicate that …

Application Of Ann Artificial Network In Slope Behavior Evaluation Using Machine Learning Technique, Marziyeh Tourani, Hadi Bahadori

NJF Intelligent Engineering Journal

In geotechnical engineering, evaluating slope stability remains a significant challenge due to inherent soil variability and environmental uncertainty. This study explores the application of Artificial Neural Networks (ANN) in predicting the factor of safety (FoS) of slopes using dimensionless input variables. An initial dataset of 349 samples was refined to 259 validated cases after removing outliers and incomplete entries. The input parameters were transformed into nondimensional forms c / γh, (tan(⌀)) / (tan (β)), and p / γh based on fluid mechanics principles to improve generalization and reduce dimensional bias. A feedforward ANN model …

Sleep Disorder Diagnosis Using Eeg Signals And Lstm Deep Learning Method, Mohammad Reza Yousefi, Reza Rahimi

NJF Intelligent Engineering Journal

Diagnosing brain disorders, particularly sleep disorders, has emerged as a critical area of research in both basic sciences and engineering. Sleep disorders encompass a range of conditions, including excessive or insufficient sleep, frequent awakenings, difficulty falling asleep, and challenges in reaching deep sleep stages. A key application of sleep disorder diagnosis lies in supporting the medical community through the development of advanced algorithms and models capable of accurately identifying these disorders based on individual brain signals, such as those captured by electroencephalography (EEG). These sophisticated approaches facilitate the creation of personalized treatment programs, paving the way for more targeted and …

A Hybrid Deep Learning Approach For Lung Diseases Classification Using Vision Transformer And Densenet, Noor Abd Alrazak Shnain, Mohammed Abdulameer Aljanabi

NJF Intelligent Engineering Journal

Lung disorders, such as pneumonia, tuberculosis, and COVID-19, remain significant global health issues. Accurate and early detection is of paramount significance to ensure effective treatment and control. Conventional CNN models like DenseNet have shown superior performance in the classification of medical images, whereas Vision Transformers (ViTs) have recently gained momentum as formidable models to capture global context information. Each of these models, however, has limitations when operated standalone. This manuscript suggests an innovative hybrid deep learning framework that synergizes the benefits of both ViT and DenseNet to advance lung disease diagnosis using chest X-ray images. The hybrid model uses both …

Post-Translational Modifications Of Extracellular Matrix Domains As Prognosticators Of Hepatocellular Carcinoma Via Integrative Serum And Spatial Proteomics, Jade K. Macdonald

MUSC Theses and Dissertations

Hepatocellular carcinoma (HCC) remains a major global health challenge, with rising incidence, high mortality, and a five-year survival rate below 20%. Approximately 90% of HCC cases arise in cirrhosis, a stromal disease characterized by excessive deposition and remodeling of collagenous extracellular matrix (ECM). As HCC progresses, alterations in ECM composition and spatial distribution influence disease progression, emphasizing the potential of the ECM as a biomarker for HCC prognosis and treatment response. However, ECM proteomic analysis is challenging due to complex post-translational modifications, crosslinking, and insolubility. This dissertation molecularly and spatially characterizes the stromal landscape in and around HCC tumors in …

A Fluorescence Lifetime-Based Flim-Timer For Measuring The Protein Turnover Of Transcription Factor Nrf2 In Live Cells, Dina Dikovskaya, Claudia Bento-Pereira, Kanade Shiga, Andrea Corno, Maureen Higgins, Rachel Toth, Adrian T. Saurin, Albena T. Dinkova-Kostova

Peninsula Medical School

Measuring protein turnover in cells has been greatly assisted by fluorescent timers (FT). However, FT quantification requires relatively high fluorescence intensity samples, prohibiting their use for proteins with low or non-uniform expression like transcription factor Nrf2, the master regulator of redox homeostasis. To visualise changes in stability/turnover of Nrf2, we constructed a genetically encoded tag combining sfGFP and mCherry and used intensity-independent Fluorescence Lifetime Imaging (FLIM) to measure Förster Resonance Energy Transfer (FRET) within the tag (named FLIM-timer). We show that the ability of mCherry to act as a FRET-acceptor develops as the protein matures, allowing the use of FLIM-FRET …

Guatemalan Maya Women’S Attitudes Towards Biomedical Contraception After Migrating To The United States, Shreenaya Prabu, Iris Parshley, Robert Malkin

Maya America: Journal of Essays, Commentary, and Analysis

Medical providers in the United States who discuss the use of contraceptives with Maya women migrants might discover strong reluctance and complex beliefs about the social and physical benefits of biomedical contraceptive measures. This research report presents information about contraceptive usage among Maya in Guatemala and how usage or attitudes toward contraception might have changed while living in the United States. The research team, during 2024, interviewed six adult female Guatemalan Maya refugees over Zoom to understand their thoughts toward contraceptive use before and after migrating. Discussion points included: lack of sexual education in Guatemala, health concerns arising from using …

Full Issue - Maya America V7, I1, Maya America Editors

Maya America: Journal of Essays, Commentary, and Analysis

Full issue of Maya America, volume 7, issue 1.

Ocean And Coastal Acidification Monitoring Priorities For The Northeast Us And Eastern Canada, Christopher Hunt, Austin Pugh, Jake Kritzer, Samantha Siedlecki, Kumiko Azetsu-Scott, Parker Gassett, Diane Lavoie, Adam Pimenta, Amy Trice, Elizabeth Turner

Faculty Publications

The Interagency Working Group on Ocean Acidification Monitoring Prioritization Plan 2024 calls for Coastal Acidification Networks to identify the ocean and coastal acidification (OCA) monitoring needs most important for their regions. The Northeast Coastal Acidification Network (NECAN) organized a webinar series to study regional needs, which culminated with a workshop in November 2023. This workshop led to the identification of six priority new Monitoring Needs in addition to the maintenance of current monitoring efforts:

• Improve spatial and temporal scale of monitoring co-located OCA variables and biological measurements to better resolve variability of acidification dynamics in concert with biological processes …

Advanced Robotic Multimodal Imaging With Real-Time Motion Compensation For Dynamic Structural Health Monitoring, George Papaioannou, Christos Mitrogiannis, Mark Schweitzer, Maria Pappa, Pegah Khosravi, Apostolos Karantanas, Chris Ruberg, Shawn Owens, Nikolaos Michailidis

Publications and Research

Modern composite materials promise superior performance and load-bearing capabilities, yet evaluating their structural integrity remains challenging. Current testing methods, such as visual, thermographic, ultrasonic, optical, electromagnetic, terahertz, shearography, X-ray, and neutron imaging, are hampered by long scan durations, limited field of view, suboptimal accuracy, and high costs, particularly when applied to large structures.

This paper addresses these issues by introducing a novel robotic multimodal imaging system that overcomes the limitations of traditional methods. This system dynamically captures both static and dynamic properties of materials using advanced motion compensation techniques. By integrating multiple radiographic modalities into a coordinated robotic platform, it …

Highly Oligomeric Drp1 Strategic Positioning At Mitochondria-Sarcoplasmic Reticulum Contacts In Adult Murine Heart Through Actin Anchoring, Celia Fernandez-Sanz, Sergio De La Fuente, Zuzana Nichtova, Marilen Federico, Stephane Duvezin-Caubet, Sebastian Lanvermann, Hui-Ying Tsai, Yanguo Xin, György Csordás, Wang Wang, Arnaud Mourier, Shey-Shing Sheu

Department of Medicine Faculty Papers

Mitochondrial fission and fusion appear to be relatively infrequent in cardiac cells compared to other cell types; however, the proteins involved in these events are highly expressed in adult cardiomyocytes (ACM). Therefore, these proteins likely have additional non-canonical roles. We have previously shown that DRP1 not only participates in mitochondrial fission processes but also regulates mitochondrial bioenergetics in cardiac tissue. However, it is still unknown where the DRP1 that does not participate in mitochondrial fission is located and what its role is at those non-fission spots. Therefore, this manuscript will clarify whether oligomeric DRP1 is located at the SR–mitochondria interface, …

Phosphorylation Deficient Inducible Camp Early Repressor (Icer) Modulates Tumorigenesis And Survival In A Transgenic Zebrafish (Danio Rerio) Model Of Melanoma, Justin Wheelan, Melissa Rose Spigelman, Angelo Cirinelli, James Reilly, Carlos A. Molina

Department of Biology Faculty Scholarship and Creative Works

Melanoma, the most lethal form of skin cancer, is commonly associated with mutations in the BRAF gene, particularly BRAF^V600E, which drives tumor proliferation via the ERK1/2 signaling cascade. While BRAF inhibitors initially demonstrate efficacy, therapeutic resistance remains a significant challenge. Emerging evidence implicates the cAMP signaling pathway, particularly the cAMP response element-binding protein (CREB) and its repressor, inducible cAMP early repressor (ICER), in melanoma progression and drug resistance. ICER, a transcriptional repressor regulated via Ras/MAPK-mediated phosphorylation and ubiquitination, is degraded in melanoma, undermining its tumor-suppressive role. In a braf^V600E; p53 (loss of function) transgenic zebrafish ( …

Disentangling The Effect Of Criminal Legal System Involvement On Mental Health In The United States, Pui Ying Chan

Dissertations and Theses

BACKGROUND: Interactions with the criminal legal system (CLS), such as through arrest or incarceration, are relatively common in the United States (US) and are increasingly recognized as social risk factors for poor mental health. Previous studies have reported a positive association between CLS involvement and poor mental health, with some theoretical frameworks, including the ecosocial theory and the stress process paradigm, supporting a plausible causal relationship. However, prior research has significant limitations in providing casual evidence given possible reverse causation in cross-sectional studies and insufficient consideration for the dynamics between CLS exposures, mental health status, and related confounders over time …

A Meta-Analysis Of Historic Drinking Water Emergency Events Reported In News Articles, Victor Yu, Haiying Wang, Weiwei Mo

Faculty Publications

This study seeks to investigate historical drinking water contamination events that reported by news media to find the key determinants of their outcomes, including the duration and economic cost, and to quantitatively describe the characteristics of past contamination events. ProQuest Global Newsstream and Regional Business News were used to identify an initial list of events and supplemented by additional search on specific information including the response actions, economic cost, and more. These events were analyzed using descriptive statistics, correlation analysis, and regression modeling. Smaller community water systems were found to be more frequently impacted by contamination events. Use restrictions, except …

Protocol For Vibratome Sectioning, Immunofluorescence, And S-Phase Labeling Of Inner Ear Organoids, Jiayi Wu, Stefan Heller, Maggie S. Matern

Biology Faculty Publications

Inner ear organoids represent a potentially inexhaustible source of otic tissues, including sensory hair cells and supporting cells, for in vitro manipulation. Here, we present a protocol for labeling S-phase entry of cells in inner ear organoids using 5-ethynyl-2′-deoxyuridine (EdU), followed by fixation and vibratome sectioning. Nuclear EdU is then detected alongside protein markers of interest via immunofluorescence. This workflow enables the visualization of cell and tissue morphologies within developing organoids and assessment of how different manipulations affect cell proliferation.

Solving A Nonhomogeneous Differential Equation And Error Analysis, Tayler Braga, Maryam Khazaei Pool

Faculty Research, Scholarly, and Creative Activity

Linear nonhomogeneous differential equations appear in many physical, biological, and engineering systems where input or forcing terms act on otherwisse linerar dynamics. Solving such equations helps model real-world phenomena, ranging from population growth under external influencse to the otion of damped systems with external forcing. The ability to compare exact olutions with numerical and matrix-based approximations is crucial for understanding th accuracy and limitations of each approach.

Executive Functioning And Mental Health In Emerging Adults: Type 1 Diabetes Status As A Moderator, Hannah Margot Mcdaniel

Theses and Dissertations

Emerging adults (ages 18-26) with type 1 diabetes mellitus (T1DM) are especially vulnerable to diabetes management challenges when taking on increased disease responsibility during this critical period of development. They may experience higher cognitive and psychological demands compared to peers. The study examined whether having T1DM moderated the association between executive functioning (EF) and mental health symptoms (i.e., depression, anxiety, stress, and disordered eating) in emerging adults. Exploratory analyses investigated the associations between EF, disease management, and mental health symptoms. Participants were 35 emerging adults with T1DM and 35 healthy controls. Participants completed questionnaire measures of mental health and performance-based …

Scent Of The South: Fragrance And Identity In The 19th Century, Sarah Donahua Peters

Theses and Dissertations

This thesis explores the role of personal fragrance as a cultural marker in the 19th-century American South, examining how scent reflected identity, status, and social norms in a region shaped by colonialism, capitalism, and religious influence. Drawing on historical sources, botanical knowledge, and public history practices, the project traces the evolution of fragrance from home-crafted perfumes to mass-marketed products, highlighting how access to scent was shaped by race, class, and gender. Central to the thesis is a public history exhibition designed for the Esse Purse Museum in Little Rock, Arkansas, which invites visitors to engage with scent as a medium …