Microbial Communities And Host Plants Of Megachilidae In The Greater Grand Forks Area (Ggfa), Sara Margit Wallace

Theses and Dissertations

Although Megachilidae are important pollinators in the United States, there is still much to learn about the health and functionality of these bees. Megachilids are threatened by competition from non-native pollinators and other environmental factors. The species distribution and composition of the gut microbiome in megachilid bees are not well known for the Greater Grand Forks Area (GGFA). My research seeks to answer the questions: what megachilid species are in the GGFA, where are they, and what symbionts are in their gut microbiomes? I collected and identified solitary bee specimens from North Dakota and Minnesota, dissected their gut contents, and …

Exploring Human Centered Design Thinking To Develop Career And Technical Education (Cte) Programs For Incarcerated Individuals: Development And Pilot Of A Faculty Survey Instrument At A Utah Technical College, Taija Jackson, Michael Pate

Journal of Research in Technical Careers

Postsecondary Career and Technical Education (CTE) institutions are confronted with growing demands to improve efficiency while boosting student outcomes and achieving performance metrics for funding. As enrollment competition grows and funding sources change, technical colleges are looking to develop new programs and identify untapped student populations such as incarcerated individuals. Instructors of postsecondary CTE technical programs often bring industry experience but typically lack formal teaching training unlike their secondary CTE instructors which may create variable teaching success and impacts both teacher confidence and student achievement, especially for at-risk populations such as incarcerated individuals. This research explores faculty perspectives on human-centered …

Closing The Gap: Educational Research On Secondary Traumatic Stress In The Healthcare Workplace, Natalie Noel Tracy

Biennial Convention (CONV)

Secondary traumatic stress (STS) is a post-traumatic stress disorder (PTSD) like phenomenon characterized by biological, emotional, and psychosocial symptoms resulting from direct exposure to someone’s traumatic experience frequently occurring in caring-based work environment. First defined in 1995 by Charles Figley, STS is classified using the same categories as PTSD: Avoidance, hyperarousal, and intrusion. Avoidance consists of numbness to or lack of participation in usual interests and avoidance of triggers that serve as reminders of the indirect trauma experienced. Hyperarousal occurs when a person becomes highly alert or has a “fight or flight” response to a triggering experience related to a …

Deficiencies In Non-Emergency Medical Transportation For Aging Rural Residents Of Iosco County, Michigan, Richard L. Douglass, Donald Stansloski, James Mortimer, Sam Purdy, Dale Evilsizer, Scott Rice

Michigan Journal of Public Health

Introduction: The rural aging population in Michigan struggles to access medical care often available only at significant distances due to limited health system resources and provider shortages. Access to routine care is challenged by lack of non-emergency medical transportation (NEMT). The Iosco County Commission on Aging provides home-delivered meals to eligible county residents. The Rural Health Group investigated the needs of home-delivered meal recipients (aged ≥60 years) for healthcare transportation access. Utilizing data from this investigation, we examined if medical care needs were hindered by problems with transportation in rural Iosco County, Michigan.

Methods: We conducted a cross-sectional survey with …

Physical Therapy Direct Access To Unrestricted Access In The State Of Michigan- Is Michigan Ready To Embrace Change? A Policy Analysis, Erica M. Fernandes, Mary D. Lagerwey, Kieran Fogarty

Michigan Journal of Public Health

Michigan’s Direct Access Provision of 2015 (Enrolled Senate Bill No. 690, 201), which aimed to increase patient access to physical therapy and reduce waiting times, has created hurdles for patient access and for physical therapy as an autonomous profession. The purposes of this policy analysis were: 1) to analyze the Direct Access (DA) policy including examining existing challenges resulting from implementation, 2) provide recommendations for implementing an alternative policy, and 3) outline the benefits of the alternative policy. The DA policy with provisions was examined using the Patton and Sawicki (1993) framework, which provides a basic approach that …

Engineering Risk And Indigenous Resilience Toward Intelligent Decision Support For Mining In Ancestral Lands, Izech Brian O. Edwin, King Harold A. Recto

Electronics, Computer, and Communications Engineering Faculty Publications

Mining within ancestral territories reveals an ongoing conflict between technical viability and Indigenous consent. This collaborative study, conducted by an engineering scholar and a policy researcher of Indigenous heritage in Benguet among the Ibaloi and Kankanaey peoples, investigates how risk transparency and policy alignment can bridge existing divides, while formulating principles applicable to other Indigenous mining contexts. The study provides an initial evaluation of the feasibility of a proposed large-scale mining operation within a risk-informed, data-driven governance framework. Three elements of engineering risk were assessed utilizing literature-derived and proxy parameters: (1) Land degradation is indicated by a Soil Productivity Index …

Metabolomic Signatures Of Prediabetes In Mexican Americans: The Role Of Genetics And Macronutrients, Shinhye Chung, Charles Evans, Charles F Burant, Han Chen, Bing Yu, David Aguilar, Eric L Brown, Craig L Hanis, Goo Jun

Faculty, Staff and Student Publications

Prediabetes is one of the main health concerns in public health, and various etiological factors contribute to its onset. This study aimed to evaluate genetic associations and gene‐macronutrient interaction with prediabetes‐related metabolites to understand how genetic variation and dietary intake contribute to dysglycemia. We analyzed a total of 482 self‐identified Mexican American participants recruited from Starr County, Texas in 2018‐2019. Untargeted metabolomic profiling was performed using LC‐MS. Nutrient densities of six macronutrients were derived from a 106‐item food frequency questionnaire. Genetic associations for each metabolite were tested using Generalized linear Mixed Model Association Tests (GMMAT). Gene‐macronutrient interactions on prediabetes‐associated metabolites …

Overview Of Huntington's Disease And Emerging Treatment Strategies: A Narrative Review, Alexis J. Vega, Gabriel V. Hernandez, Pearse A. O'Malley, Connor J. Robin, Amanda N. Parra, Giustino Varrassi, Sahar Shekoohi, Alan D. Kaye

School of Medicine Faculty Publications

Huntington's disease (HD) is an autosomal dominant, progressive neurodegenerative disorder caused by a cytosine-adenine-guanine trinucleotide repeat expansion in the huntingtin (HTT) gene. The symptoms of HD include severe motor dysfunction, cognitive issues, and emotional dysregulation. These combined issues are not only debilitating but also lead to depression/anxiety, increased suicide rates, and caregiver burnout. Our narrative review summarizes several recent studies examining the efficacy and differences among emerging treatment strategies for HD. A systematic search of peer-reviewed literature was conducted, focusing on recent studies that describe molecular genetic manipulation of the HTT gene/huntingtin protein. The results of our narrative review reveal …

Clinical And Genomic Characterization Of Serratia Bloodstream Infections, Roberta Monardo, Lawrence P Park, Felicia Ruffin, Phillip Cox, An Q Dinh, Rachel E Korn, Parisa Farahani, Marco Ripa, Antonella Castagna, Blake M Hanson, Vance G Fowler, Joshua T Thaden, Stacey A Maskarinec

Faculty, Staff and Student Publications

Background: Previously considered a rare opportunistic pathogen, Serratia is now an emerging cause of bacteremia. We compared the risk factors and outcomes of patients with Serratia bacteremia relative to other Enterobacterales in a cohort of hospitalized patients.

Methods: Patients were prospectively enrolled from 2002-2021 at Duke University. Characteristics and outcomes were compared among bacteremic patients due to Serratia and other Enterobacterales. Regression models were used to determine features associated with outcomes. Whole genome sequencing (WGS) was performed to characterize the phylogenetic diversity of Serratia isolates associated with device infections.

Results: Of 2676 patients, 173 (6.5%) had Serratia bacteremia. Among patients …

Neighborhood-Level Social Vulnerability, Race And Ethnicity, And Stage At Diagnosis: A Population-Based Study Of 12,957 Patients With Oral Cavity Cancer, Guillermo A Tortolero, Erich M Sturgis, Jose-Miguel Yamal, Melissa F Peskin, Cici Bauer, Caitlin C Murphy

Faculty, Staff and Student Publications

Background: Oral cavity cancer (OCC) is marked by racial and ethnic disparities. We examined these differences in OCC stage at diagnosis and whether neighborhood-level social vulnerability, a measure of susceptibility to hardship at the census-tract level, modifies disparities.

Methods: We identified adults newly diagnosed with OCC (1995 to 2020) from the Texas Cancer Registry. We estimated the association between stage at diagnosis and race and ethnicity using logistic regression and assessed effect modification by neighborhood-level social vulnerability.

Results: The sample included 12,957 reported OCC cases, 77% non-Hispanic White, 6% non-Hispanic Black, and 14% Hispanic patients, and 47.4% late stage at …

"Reeling In" The Governance Problem: How To Achieve Sustainable Fisheries On An International Scale, Louis Morais-Andrade

Brooklyn Journal of International Law

In 2025, it was estimated that over 35% of international fish stocks were overfished, risking severe depletion of high seas fisheries. In general, countries have control over fisheries within 200 nautical miles of their coastal border. Many countries have established fishery conservation laws to help prevent overfishing. Some examples of the largest domestic fishing laws are the Magnuson-Stevens Fishery Conservation and Management Act and the European Union’s Common Fishery Policy (MSA), which functions as an international policy that governs all of the United States’ fisheries and the European Union’s Common Fisheries Policy (CFP). Concurrently, the United Nations has also opened …

Decomposition Processes And Insect Indicators In Forensic Investigations, Eli Taylor

University Honors Theses

Insects play an essential biological and ecological role in human life, and their predictable relationship with decomposing remains has positioned them as a cornerstone of modern forensic science. Since the early 20th century, forensic entomology has provided critical evidence for estimating the postmortem interval (PMI), relying on patterns of colonization, species succession, and insect developmental timelines. Accurate PMI estimations are vital to medicolegal investigations, influencing the interpretation of biological evidence, guiding resource allocation, and informing determinations of cause and manner of death. However, insect activity is profoundly shaped by environmental and ecological variables, including temperature, humidity, seasonal shifts, and geographic …

Context Dependent Regulation Of Dicer Function During Oocyte-To-Embryo Transition, Nick A. Newkirk

Dissertations and Theses (Open Access)

Over two decades ago, Dicer was discovered as the primary enzyme responsible for the generation of small RNAs known as the microRNAs (miRNAs) and short interfering RNAs (siRNAs). Spatiotemporal regulation of Dicer-dependent small-RNA biogenesis affects many aspects of biology, including aging, disease progression, fertility, and cancer. Interestingly, a distinct population of Dicer-dependent small RNAs, known as the endogenous siRNAs (endo-siRNAs), are uniquely abundant in the germ cells and gametes, and are critical for normal fertility. Despite their function regulating new life, the mechanisms controlling their biogenesis and subsequent germ cell functions remain poorly defined. Work to parse these mechanisms in …

Capsinoids, Non-Pungent Trpv1 Agonists, Induce A Mild Hypothermia That Provides Short- And Long-Term Neuroprotection After Ischemic Stroke, Alexander Andersohn, Alexander Andersohn

Dissertations and Theses (Open Access)

Therapeutic hypothermia (TH) has demonstrated neuroprotection in instances of cardiac arrest and neonatal hypoxia/ischemia but faces different challenges in application to stroke due to the activation of cold defense mechanisms in conscious patients. This dissertation examines the efficacy and specificity of capsinoids (a purified mixture of capsiate and dihydrocapsiate) to induce a sustained mild hypothermia in conscious mice that is neuroprotective after stroke. Capsinoids function as TRPV1 agonists. However, unlike capsaicin, capsinoids are vulnerable to esterase-mediated breakdown, thus significantly restricting their action to the site of delivery. We showed that capsinoids delivered intraperitoneally (IP) to mice induced a TRPV1-dependent drop …

Functional Analysis Of Ftsa-Mediated Recruitment Of Ftsk In Escherichia Coli, Abbigale Perkins

Dissertations and Theses (Open Access)

Bacterial cytokinesis requires precise coordination between cytoplasmic and periplasmic components of the divisome to ensure that membrane constriction and chromosome segregation occur in synchrony. The actin-like protein FtsA plays a central role in this process by tethering FtsZ to the membrane and recruiting downstream division proteins. Despite its essential function, the mechanisms by which FtsA coordinates divisome activation and the recruitment of late-acting proteins remain incompletely defined. To address this, the role of FtsA in activating septal synthesis and recruiting the DNA translocase FtsK was examined using mutational analysis in Escherichia coli.

The FtsA^{M96E R153D} variant, which has …

Orthogonal Comparison Of Nuclear And Mitochondrial Clonal Architectures In Hematologic Malignancies, Nehali Shah

Dissertations and Theses (Open Access)

Acute myeloid leukemia (AML) is a hematologic malignancy characterized by accumulation of mutations that disrupt hematopoietic differentiation and promote clonal expansion. Understanding how these mutations arise and evolve is essential for improving diagnosis, prognosis, and treatment stratification. Current methods are limited by either restricted genomic coverage (targeted panels) or low throughput and high cost (in single-cell whole genome sequencing, scWGS). An emerging alternative is the use of mitochondrial DNA (mtDNA) mutations as clonal markers.

This study aims to determine whether mitochondrial-derived clonal architectures correlate with nuclear-derived clonal architectures in AML, thereby evaluating mtDNA as a scalable orthogonal tool for lineage …

Maternal Folic Acid Supplement Use, Folate Intake, And Preterm Birth Among Infants With Spina Bifida, Renata H Benjamin, Katherine L Ludorf, Rachel P Allred, Jenil R Patel, Wendy N Nembhard, Gary M Shaw, Suzan L Carmichael, Tania A Desrosiers, Eirini Nestoridi, Eva M Williford, A J Agopian

Faculty, Staff and Student Publications

Background: We sought to assess the extent to which folic acid supplementation and dietary folate intake are associated with preterm delivery among infants with spina bifida.

Methods: We conducted a retrospective population-based study using the National Birth Defects Prevention Study (NBDPS; 1999-2011) and the Birth Defects Study To Evaluate Pregnancy exposureS (BD-STEPS; 2014-2019). We utilized robust Poisson regression to calculate the risk ratio (RR) and 95% confidence interval (CI) for the associations between preterm birth (< 37 weeks) and maternal use of folic acid-containing supplements, dietary folate intake quartile, and a combined variable accounting for supplementation and dietary folate status.

Results: Among 1199 infants with spina bifida (1011 in NBDPS, 188 in BD-STEPS), 217 (18.1%) were born preterm. There were no statistically significant associations …

Assessing Response Diversity Of Spawning Conditions For Native Trout Among Streams To Interannual Changes In Snowpack, Autumn Kaye Zierenberg

Undergraduate Honors Capstone Projects

Stream habitat suitability for fish is controlled by physical, chemical and biological conditions impacted by interannual changes in climate. Intermittent streams are particularly sensitive to climatic variation and have long been overlooked as potential spawning habitats for fish and other riverine species. Spawning suitability in stream habitats is strongly influenced by connectivity to perennial habitats, the duration of surface flows and water temperatures, each of which are driven by annual snowpack and discharge conditions. Here, we examine how the spawning habitat suitability in different tributaries within a single river basin respond to changes in annual snowpack conditions. Specifically, we examined …

Bundled For The Beyond: Material Analysis Of A Canine Mummy Bundle For Contextualizing And Conserving Legacy Objects, Sascha Anna-Rose Baldauf

Undergraduate Honors Capstone Projects

Legacy collections¹ and objects with little context are often untapped opportunities for both museums and museum studies students. The purpose of this study is to display how students can utilize object research to engage in experiential learning and develop their skillset in scientific methodologies and disseminating findings, while also offering museums the opportunity to develop relationships with research facilities and benefit from student research findings. This study demonstrates the utility of student research by investigating an Egyptian canid mummy bundle, dating to the Late Period² or early Ptolemaic Period³, received by the Museum of Anthropology at …

Metabolic Dysfunction Caused By Dietary Oxylipins And The Environmental Toxicant Pfos, Jazmine Alexis Eccles-Miller

All Dissertations

Disruption of fat metabolism is a hallmark of metabolic disease. A liver enzyme, Cytochrome P450 2B6 (CYP2B6) is an anti-obesity enzyme that produced several omega-3 and omega-6 fatty acid metabolites that could contribute to metabolic diseases such as fatty liver disease. Two of these metabolites, 9-HODE and 9-HOTrE, may be associated with fatty liver. Mice were exposed to 9-HODE and 9-HOTrE to investigate whether they contribute to fatty liver disease under fasting conditions. A transgenic mice model expressing the human enzyme CYP2B6 was also fasted to determine if CYP2B6 leads to increased fatty liver under fasting conditions compared to a …

Exploring Halogen Bonding Interactions In Cocrystals And Deep Eutectic Solvents, Madhushi Bandara

All Dissertations

Halogen bonding is a noncovalent intermolecular interaction analogous to hydrogen bonding that occurs between an electrophile and a nucleophile. This is often observed in compounds containing heavy halogens such as iodine and bromine. When these halogens are covalently bonded to another atom or molecule, preferably to an electron-withdrawing moiety, an electrophilic region called a sigma hole is created on the halogen atom on the extension of the covalent bond.

The first study of this dissertation presents a comprehensive characterization of eight newly synthesized triiodide salts using single-crystal X-ray diffraction, powder X-ray diffraction, thermal analysis (DSC and TGA), and Raman spectroscopy. …

Synthesis And Characterization Of Chemically Recyclable Polypropylene-Based Covalent Adaptable Networks, Andrii Tiiara

All Dissertations

Polyolefins (POs) are inexpensive engineering materials with excellent physical and mechanical properties, accounting for nearly 60% of all thermoplastics. However, large-scale recycling remains limited, with only ~15% undergoing mechanical recovery. This dissertation addresses this challenge by fragmenting and functionalizing polypropylene (PP) chains to generate macromonomers and employing them, along with industrial reactive PP macromonomers, to synthesize, depolymerize, and repolymerize chemically recyclable polypropylene-based polyolefins (CR-POs). These CR-POs incorporate ester linkages that form covalent adaptable networks (CANs), maintaining a gel fraction of ~70% while remaining melt-reprocessable via extrusion and compression molding.

First, the fragmentation of PP is investigated by microwave irradiation. Microwave …

Exploring Glycosomes As Therapeutic Targets For Trypanosoma Brucei, Sabrina Sutton Pizarro

All Dissertations

Trypanosoma brucei is a protozoan parasite which causes human African trypanosomiasis and the livestock wasting disease, nagana. These parasites have highly specialized peroxisomes, called glycosomes, which house a variety of essential metabolic pathways, including glycolysis. Glycolysis is the sole means of energy production in the infective life stage of T. brucei making glycosomes and the glycolytic pathway promising targets for therapeutic strategies. In this work, we have studied the glycosome as a therapeutic target from multiple perspectives. The first portion of this work addresses drug trafficking. The efficacy of small-molecule inhibitors of glycosomal enzymes is restricted by the rate of …

Purifying Circular Rna Therapeutics, Karen Guillen-Cuevas

All Dissertations

Circular RNA (circRNA) has emerged as a promising therapeutic modality due to its enhanced stability and resistance to exonuclease degradation compared with linear mRNA. However, its stability and functionality depend strongly on achieving high purity, and current methods such as size-exclusion chromatography suffer from peak overlap, low yields, and poor scalability. This dissertation addresses these limitations by developing ultrafiltration-based strategies to purify circRNA directly from in vitro transcription (IVT) and self-splicing (circularization) reactions. First, the feasibility of ultrafiltration was demonstrated using polyethersulfone membranes, where sieving coefficients and critical flux values of circRNA, precursor RNA, and nicked RNA were measured to …

Examining The Roles Of Embodiment And Theory Of Mind In Shaping User Perceptions Of Llm-Driven Conversational Agents, Elizabeth A. Schlesener

All Dissertations

Large Language Models (LLMs) have advanced conversational agents, enabling natural, human-like interactions in domains such as education, programming, and workplace collaboration. Yet, user distrust persists over privacy, accuracy, and bias. As developers work to mitigate these issues and human-AI collaboration expands, reinforcing trust in LLM-driven systems is essential. To address this problem, this dissertation explores the role of anthropomorphic form in LLM-driven conversational agents and its impact on user perception.

According to the familiarity thesis, humans attribute human-like characteristics to nonhuman entities — a process known as anthropomorphism — to better comprehend unfamiliar phenomena, based on the assumption that they …

The Life Significance Of Summer Outdoor Program Employment During Emerging Adulthood, Austin M. Souto

All Dissertations

Seasonal employment in outdoor programs—encompassing camps, guiding services, and similar organizations—plays a vital role in delivering structured outdoor experiences, yet little research has explored its enduring effects on staff over the course of their lives. This dissertation examined the long-term significance and influence of seasonal outdoor work during emerging adulthood. Guided by Significant Life Experience and Life Course Theory, an exploratory sequential mixed-methods design was used. In the qualitative phase, interviews revealed that seasonal work influenced life trajectories by contributing to identity formation, career discovery, ways of relating to others, and the navigation of trade-offs. Seven mechanisms—opportunities for growth, connection …

Surface Reaction And Transportation Of Radium-226 In Marine Systems, Jiusi Wang

All Dissertations

Historical uses of radium (Ra)-containing self-luminous paint, such as on gauges and dials in naval ships, have led to the potential for such paint, and thus Ra, to be released into the marine environment. Radium-226 (²²⁶Ra) behavior in marine systems is not fully described in the literature, presenting a knowledge gap in the process of evaluating potential ecological impact of Ra in marine systems. This study explores various mechanisms contributing to Ra environmental transport in marine systems on a laboratory scale, including leaching, sorption, and diffusion. ²²⁶Ra leaching from watch hand dials containing radioactive luminous paint is …

Legitimacy, Learning, And Launch: Exploring Relationships, Community, And Connection In An Undergraduate Research Experience, Randi J. Sims

All Dissertations

Undergraduate research experiences (UREs) are critically important methods for integrating undergraduate students into legitimate scientific practices. While widely celebrated for producing positive outcomes such as increased self-efficacy, research skills, and STEM retention, less is known about the processes through which undergraduate mentees become integrated members of their research groups and the broader scientific community. This dissertation presents findings from both a systematic literature review and a quantitative ethnographic study of a biological sciences URE at a southeastern R1 predominantly white institution (PWI). Grounded in communities of practice and legitimate peripheral participation frameworks, this study explores how undergraduate researchers build relationships, …

The Influence Of Tert-Butylhydroquinone (Tbhq) On Acod1/Irg1-Driven Inflammatory Pathways, Alyssa M. Whisel

All Dissertations

The demand for processed foods has led to increased use of synthetic phenolic antioxidants (SPAs), notably tert-butylhydroquinone (TBHQ), to inhibit lipid oxidation and prolong shelf life. While TBHQ is deemed safe at regulated levels, concerns arise from studies indicating potential adverse effects such as oxidative stress, genotoxicity, and impaired immune function. To investigate TBHQ's immunomodulatory effects, RAW 264.7 murine macrophages were pre-treated with TBHQ and subsequently stimulated with lipopolysaccharide (LPS) or polyinosinic-polycytidylic acid (poly I:C, PIC) to simulate bacterial or viral challenges. Results demonstrated that TBHQ at high non-cytotoxic doses reduced nitric oxide and IL-10 production while promoting phagocytosis and …

Atomic-Scale Engineering Of Dilute Pd–Cu Alloys For Selective Electrocatalytic Cox Reduction, Zehua Jin

All Dissertations

The excessive release of carbon dioxide (CO2) into the atmosphere has become a major cause of global warming and ocean acidification, threatening the sustainability of human life on Earth. To address this challenge, scientists are exploring ways to convert CO2 into useful fuels and chemicals instead of simply capturing and storing it. One promising approach is electrocatalysis, which uses electricity—potentially from renewable sources—to drive chemical reactions that transform CO2 under mild, environmentally friendly conditions.

This research aims to understand how the arrangement of atoms in metallic catalysts affects the conversion of CO2 and carbon monoxide (CO) into valuable products such …