Digital Commons Network^™

Recovery And Characterization Of Moraxella Species From Bovine Specimens, Laura G. Leger

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

The family Moraxellaceae is made up of a diverse group of 24 named species that fall into three phylogenetic groups. While most Moraxella spp. act as commensal respiratory and/or ocular flora of mammals, some are associated with disease. One of these diseases, infectious bovine keratoconjunctivitis (IBK), has significant welfare and economic impacts to both cattle and cattle producers. Moraxella bovis is the only experimentally determined cause of IBK, however, an association of Moraxella bovoculi with disease has been proposed. Currently, available vaccines do not have high levels of efficacy in the field. Culture based methods are the gold standard for …

Amplifying Mother Leaders’ Voices: A Feminist Inquiry Into Gender Inequity In Higher Education Leadership, Katherine Bard

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Women now comprise more than half of mid- and upper-level administrators in higher education, yet they remain underrepresented in the most senior leadership roles. For mothers in leadership, this underrepresentation is compounded by gendered structures, expectations, and norms that shape their personal and professional lives in distinct ways. While scholarship has examined working motherhood broadly and gender inequity in higher education separately, little research has focused on how mother leaders experience and interpret gender inequity in higher education leadership. This study sought to address that gap by exploring the lived experiences of ten mother leaders working at research-intensive (R1) universities …

In-Field Tractor Operational Load Profile Generation In Support Of Advanced Tractor Testing In Mixedmode Power, Andrew Donesky

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation addresses the need to better characterize real-world tractor power requirements across drawbar, power take-off (PTO), and hydraulic modes to support more representative tractor testing. Conventional testing protocols, such as OECD Code 2, emphasize steady-state performance under controlled high-load conditions, which do not reflect the mixed and dynamic demands of modern field operations. To address this gap, a Tractor Instrumentation System (TIS) was developed, validated, and deployed to collect high-resolution, mixed-mode power data during planting, anhydrous ammonia application, and grain cart operations. The TIS integrates physical sensors, including custom load pins, hydraulic pressure/flow sensors, and a redesigned PTO torque …

Identification And Characterization Of Host-Modulating Effectors Encoded By The Cluster F1 Mycobacteriophage Normanbulbiejr, Bethany M. Wise, Kaylia Edwards, Cole R. Jirsa, Sabrina Abbruzzese, Amina Adebiyi, Sarika Bapat, Tyler Barnhardt, Naomi Bastiampillai, Ella Begovic, Mary G. Berchick, Gilda Bocco, John Bonoris, Elizabeth Boos, Margaret Cassady, Julia Chehab, Gene Cooper, Hunter Coyle, Joseph Davis, Natalia De La Cruz Vargas, Matthew Delach, Carly Dowiak, David Ferraro, Madison Fuller, Bootsie Glasser, Kyle Gordon, Hannah Hoch, Sarah Holleman, Luke Hood, Brett Hurrell, Nicholas Jacobs, Danny Jiang, Steven Kefalos, Salma Maher, Jocelyn Martin, Rohebot Mengesha, Daniel Merenich, Ashwin Nayak, Dasha Nesterova, Joshua Nguyen, Chukwudumebi Okonkwo, Taylor Pompan, Lauren Redwood, Christina Scanlon, Lily Schneider, Aparna Shenai, Colton Siatkowski, Lisa Strausner, Joseph Vizzarri, Jordan Watson, Angela Wilson, Catherine Wynne, Anthony Zygmunt, Michelle Kanther, Kimberly M. Payne, Richard S. Pollenz, C N. Sunnen, Danielle M. Heller

PCOM Scholarly Works

NormanBulbieJr (NBJ) is a temperate siphovirus isolated on the host Mycobacterium smegmatis mc²155 that encodes 102 gene products, 60 of which have no known function (NKF). Based on gene content, NBJ is classified as a Cluster F, Subcluster F1 phage and shares 70% of its encoded gene phamilies with Girr, another F1 mycobacteriophage that was recently analyzed in a genome-wide overexpression screen and found to encode 29 diverse gene products capable of inhibiting growth of M. smegmatis. Similar functional screens in other mycobacteriophages have uncovered a growing repertoire of diverse, phage-encoded bacterial growth inhibitors, providing prime candidates …

Predictive Modeling Of Listeria Monocytogenes Growth In Ready-To-Eat Meat Products, Manjari Singh

Graduate Theses and Dissertations

Listeria monocytogenes remains a significant food safety concern in ready-to-eat (RTE) meat and poultry products due to its persistence under refrigerated and stressed conditions. Predictive models play a vital role in risk assessment and food safety management; broth models are highly conservation & results in overprocessing. This thesis focuses on developing and validating meat-specific predictive models for Listeria monocytogenes growth and lag for RTE beef, pork, and poultry products. Matrix-specific secondary models were built to predict the maximum specific growth rate (μmax) of Listeria monocytogenes under varying environmental conditions, including temperature, pH, water activity, salt, organic acids, and nitrites. Growth …

Southern / Appalachian Migrants And Urban Development Politics In Uptown Chicago, 1950-1972, Charles Wayne Dowdy

Electronic Theses and Dissertations

This thesis investigates how ideas of interregional migration were used in service of urban renewal agendas in Uptown Chicago from 1950 to 1972. It argues that The South and Appalachia as regional ideas were impactful in postwar midwestern urban development politics, even as regional identity was less powerful as an adhering force for actual migrants in comparison with factors of race and class. Chapter 1 examines the development and use of the urban enclave model of interregional migration by the Uptown Chicago Commission in the late 1950s. It argues that anxieties around suburbanization and urban decline fueled the production of …

Glp-1ra Vs. Sglt-2i: Preventing Kidney Function Deterioration Among Patients With Type 2 Diabetes And Chronic Kidney Disease, Liang-Yuan Lin

Electronic Theses and Dissertations

Background: Emerging evidence suggests that glucagon-like peptide-1 receptor agonists (GLP-1RA) may improve kidney outcomes in individuals with type 2 diabetes mellitus (T2DM). However, direct head-to-head comparisons of their renal effectiveness with sodium-glucose cotransporter 2 inhibitors (SGLT-2i)—the recommended first-line therapy for older adults with T2DM and chronic kidney disease (CKD)—remain limited.

Objectives: To compare the renal effectiveness of GLP-1RA and SGLT-2i among older adults with T2DM and CKD.

Methods: A new-user, active comparator, matched cohort study was conducted using 5% Medicare data from 2012 to 2020. Older adults (≥65 years) with T2DM and CKD who initiated either a GLP-1RA or an …

Racial And Ethnic Differences In The Associations Between Alcohol Use Disorder, Aggression, And Suicidality Among Adolescents With Ptsd, Gabrielle Armstrong

Electronic Theses and Dissertations

The comorbidity of posttraumatic stress disorder (PTSD) and alcohol use disorder (AUD) is well documented and associated with elevated risk of aggression and suicidality among certain populations. However, no research to date has examined these associations among adolescents. Using data from the National Comorbidity Survey-Adolescent Supplement (NCS-A), the present study examined whether the presence of AUD was associated with greater outward aggression, suicidal ideation, and attempts, and whether these associations differed across race/ethnicity, in a weighted sample of 737,996 adolescents with PTSD (10.3% Hispanic, 16.4% Black, 73.3% White). AUD presence was significantly associated with higher odds of breaking items, attacking …

A Road Less Traveled: Exploring The Motivators Influencing College Enrollment Decisions Among Youth Who Have Experienced Foster Care, John William Blue

Electronic Theses and Dissertations

Youth who have experienced the foster care system are an underserved student population in higher education. While higher education is an important pathway to upward mobility and civic participation, foster youth consistently face systemic barriers such as unstable placements, limited financial resources, and a lack of consistent adult guidance, resulting in significantly lower rates of college enrollment and degree attainment compared to their peers (Courtney & Okpych, 2020; Salazar, 2013). This three-manuscript dissertation investigates the challenges that youth who have experienced foster care face and the factors that influence them to pursue enrollment in four-year universities in Illinois, while also …

The Impact Of State-Mandated High-Stakes Standardized Testing On The Job Satisfaction Of Third-Grade Reading Teachers In Mississippi, Jenny Kate Ernst

Electronic Theses and Dissertations

This qualitative study examined how state-mandated standardized testing influences the job satisfaction of current and former third-grade reading teachers in Mississippi. Guided by Self-Determination Theory, Herzberg’s Two-Factor Theory, and the Job Demands-Resources Model, the research explored how accountability pressures shape teachers’ motivation, emotional well-being, and instructional autonomy. Data were collected through an open-ended online survey of current and former Mississippi third-grade reading teachers. Analysis of responses revealed four central themes: emotional strain and burnout, loss of autonomy and creativity, impact on instructional practices, and student impact. Findings indicated that teachers perceive standardized testing as a major source of stress that …

The Lived Experiences Of Lgbtq+ Individuals Living In Male Residential Spaces In The South, Bri Reda

Electronic Theses and Dissertations

While LGBTQ+ visibility on college campuses has increased, LGBTQ+ students still report negative experiences, particularly in residential environments where harassment and unwelcoming attitudes are common (Kortegast, 2017; Pryor, Ta, & Hart, 2016). Previous studies have shown that LGBTQ+ individuals often conceal their identities in residence halls due to discomfort, further diminishing their feelings of safety and connection to the campus community (Blakmon, O’Hara, & Viars, 2020; Pryor, Ta, & Hart, 2016). Despite efforts to implement inclusive policies, little research has explored the experiences of LGBTQ+ individuals in male-assigned residence halls, especially in states like Mississippi with gender-segregated housing requirements (Mississippi …

Out-Of-Pocket Costs, Treatment Pattern, And Disparities In Outcomes Among Adults With Chronic Lymphocytic Leukemia, Shishir Maharjan

Electronic Theses and Dissertations

The purpose of this dissertation was to: 1) determine out-of-pocket cost trend and the impact of Inflation Reduction Act (IRA) provisions on the out-of-pocket costs among Medicare-enrolled chronic lymphocytic leukemia (CLL) patients; 2) evaluate treatment patterns and comparative effectiveness of therapeutics classes for CLL treatment in adults; and 3) assess racial disparities and time to treatment initiation in survival outcomes among Medicare-enrolled beneficiaries with CLL.

In objective 1, a cohort study using SEER-Medicare linked data from 2006 to 2020 identified 13,356 eligible beneficiaries with incident CLL. Among them, 3,158 (23.7%) were projected to benefit from the IRA’s $2000 annual out-of-pocket …

Mechanical Property And Thermal Stability Characterization Of Additively Manufactured Chicken Feather Fiber/Polybutylene Succinate (Pbs) Green Composites And In-Situ Pore Detection From Fdm 3d Printing Videos, Md Shariful Islam

All Theses

The poultry industry generates millions of tons of chicken feathers annually, most of which are underutilized and discarded as waste materials. Chicken feather fiber (CFF), primarily composed of keratin, possesses low density, biodegradability, and favorable mechanical properties, making it a promising reinforcement for sustainable composite materials. This research primarily focused on developing sustainable bio-composites using poultry waste, chicken feather fiber, and biodegradable polymers, specifically Polybutylene Succinate (PBS), for additive manufacturing applications. Composite filaments of varying fiber loadings (0.5, 1, and 2 wt%) were produced through filament extrusion and processed into test specimens using fused deposition modeling (FDM).

Mechanical properties were …

Visualizing The Fretboard: Arpeggiated Patterns, Muscle Memory, And Neurocognitive Strategies For The 5-String Electric Bass, Matthew Alexander Woisin

Electronic Theses and Dissertations

Proficient navigation of the 5-string electric bass requires a comprehensive mastery of various techniques and a solid understanding of music theory. This includes a detailed grasp of arpeggios, chord extensions, and the development of deeply ingrained muscle memory for precise execution. This thesis introduces a structured methodology that refines existing fretboard visualization techniques and applies a unique manner of fretboard mapping, offering a more precise framework for comprehending and unlocking the full range of the instrument. By drawing insights from music theory, music education, cognitive science, neuroscience, and technology this approach is achieved by synthesizing research on visual pattern recognition, …

Llu Dentistry 2025 Winter Edition, Loma Linda University School Of Dentistry

Articulator (Dentistry)

In this issue:

4 | Dean's Message
5 | Transforming the Future of Dental Education: The Strategic Vision and Growth Plan
8 | Honoring a Legacy of Excellence: Celebrating Leif K. Bakland, DDS
12 | Transitions
14 | Welcome New Faculty
16 | Celebrating Excellence in Dentistry: 2025 ICD USA Section Annual Meeting & Convocation
18 | LLUSD faculty publish study on Dental Unit Waterline treatment in the Journal of the American Dental Association
21 | Celebrating 15 Years of PNAM Treatment at LLUSD
23 | A Journey of Healing: Shirely's Transformation
24 | Exploring Future Careers in STEM: The …

Examining The Relations Between Parenting Stress, Harsh Parenting Practices, And Children’S Internalizing And Externalizing Symptoms, Caitlin A. Wharton

Individual, Family, and Community Education ETDs

This study examines: relations between maternal parenting stress in infancy and preschool-aged children’s internalizing and externalizing symptoms; whether verbal and physical harsh parenting practices mediate these relations; and whether these relations are moderated by child gender. Research links maternal parenting stress to harsh parenting, which is a risk factor for children’s internalizing and externalizing symptoms. But limited research has examined how maternal stress in infancy affects preschool-aged children’s outcomes, or how verbal and physical harsh parenting practices independently affect these outcomes. The study data came from the Future of Families & Child Wellbeing Study. Results indicate maternal parenting stress in …

Forage Preference In Two Geographically Co-Occurring Fungus Gardening Ants: A Dietary Dna Approach, Matthew Richards-Perhatch V, Elizabeth Boshers, Matthew Greenwold, Jon N. Seal

Biology Faculty Publications and Presentations

Traditional methods of forage identification are impractical with non-leafcutting fungus gardening ants, making diet-related ecological and life history questions difficult to study. To address this limitation, we utilized dietary DNA metabarcoding on excavated ant fungus gardens to generate forage diversity metrics for the two co-occurring species Trachymyrmex septentrionalis and Mycetomoellerius turrifex. Ten fungus garden samples from each species were collected from a 60x70 m plot in East Texas. Each of the colonies we sampled was paired with a colony from the other species within 3 m of it. Plant forage diversity was assessed with chloroplast trnL primers, and insect frass …

Identifying Common Characteristics Among Female Serial Killers And The Role Of Early Childhood Abuse, Jill Marie Raichel

Walden Dissertations and Doctoral Studies

Approximately 17% of known serial killers in the United States are women, and often their crimes go undetected for years accruing a higher number of victims than their male counterparts. The purpose of this study was to identify common characteristics of female serial killers (FSKs). A multidisciplinary, holistic and developmental approach was used to guide the identification of common characteristics based on theories by Erik Erikson and Carol Gilligan as well as feminist theory. Case studies of 15 FSKs were randomly selected for participation from a list of over 100 known FSKs documented in the Radford Serial Killer database. Common …

Ethnic Identity, Parental Involvement, And Academic Outcomes: A Qualitative Study Of African International Students' Experiences, Kenanile Olivia Marie-France Bigba

College of Education and Human Sciences: Dissertations, Theses, and Student Research

This qualitative study explored how parental involvement intersected with cultural backgrounds and values through ethnic identity formation among African international students, and how these interactions influenced students' self-concept clarity, self-esteem, and subsequent academic attitudes and performance. Using hermeneutic phenomenological methodology guided by van Manen’s (2023) approach, the study conducted in-depth, semi-structured interviews with African international students (one from East Africa, one from West Africa) enrolled at a Midwest university. Through systematic thematic analysis, five interconnected themes emerged: (1) Evolving Parental Involvement, from direct guidance to distant trust, revealing how parental engagement transforms across geographical distance with financial circumstances moderating this …

Defining The Causal Contribution Of The Gut Microbiota To Cardiomyopathy Pathogenesis As A Basis For Developing Microbiome-Targeted Therapies, Xu Shi

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Heart failure (HF) is a leading cause of morbidity and mortality worldwide, placing substantial clinical and economic burdens on society. Although advances in therapy have improved patient outcomes, the biological mechanisms that drive HF onset and progression remain incompletely understood. Increasing evidence suggests that the gut microbiota, the trillions of microorganisms residing in the gastrointestinal tract, plays a key role in some forms of heart disease, especially cardiovascular disease. Moreover, gut microbial dysbiosis has been associated with systemic inflammation, metabolic imbalance, and immune dysregulation, all of which contribute to the pathophysiology of HF. Cardiomyopathy, a primary disorder of the heart …

Soil Characterization With Proximal Geophysics, Sophia M. Becker

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

High quality soil data is crucial for addressing numerous threats to human well-being such as water scarcity, food insecurity, and intensified natural disasters. True soil property values are determined by analyzing soil samples in a laboratory, but soil sampling is often expensive and time-consuming. Some in-field point sensors and remote sensing products are available to measure soil properties, but they have inherent limitations in measurement extent and resolution. Geophysical methods can fill the measurement scale gap between point measurements and remote sensing products, but various research questions remain open. This dissertation aims to advance understanding of geophysics-derived soil property measurements …

An Integrated Modeling Framework To Evaluate Circularity Of The Corn–Water–Ethanol–Beef Nexus, Heydi Han

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation examines the transition from conventional linear agricultural and food bioenergy systems toward more circular, resilient, and resource-efficient configurations. Prior research indicates that spatial and temporal variability in soil, weather, animal biological responses, and management decisions strongly influences productivity, nutrient-use efficiency, and profitability. Studies have also shown that a diversified circular system can buffer these sources of variability, improving stability and adaptive capacity. Building on these insights, the central focus of this work is to develop an integrated modeling framework to evaluate circularity within the Corn-Water-Ethanol-Beef (CWEB) nexus and explore how climate variability and management choices shape system-wide performance. …

Ai-Enabled Robotic Nitrogen Management, Ankita Kalra

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Nitrogen management in corn agriculture faces economic and environmental challenges, motivating development of precision, site-specific solutions. This dissertation presents AIR-N (AI-Enabled Robotic Nitrogen Management), a novel system that integrates cloud-based artificial intelligence with an autonomous field robot (Flex-Ro) to optimize nitrogen fertilizer application in real time. The research is driven by the need to improve nitrogen use efficiency while reducing leaching and emissions.

Comprehensive architecture (Cloud-E) was designed using Amazon Web Services to ingest multi-modal agronomic data (soil sensors, crop reflectance, weather) and host machine learning models. A Random Forest-based model was trained on historical field data to predict spatially …

Evaluating Fall And Spring Planted Cover Crops For Integrated Management Of Herbicide Resistant Palmer Amaranth And Effect On Soil Health, Vipin Kumar

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Herbicide-resistant Palmer amaranth (Amaranthus palmeri S. Watson) pose a severe threat to agronomic crop productivity in the United States, including corn and soybean production in Nebraska. Therefore, there is an urgent need for diversified and sustainable management approaches. Integrating cover crops (CCs) with herbicide programs represents a promising strategy to achieve Palmer amaranth suppression, sustain crop yields, and contribute to long-term soil health in irrigated corn–soybean production systems in Nebraska.

A meta-analysis of 41 studies and 595 paired observations demonstrated that CCs reduced Amaranthus spp. density and biomass by 37–59% depending on the growing season, with grasses and mixtures …

Synthesis And Study Of Stable Organic Radicals For Mri Contrast Agents And New Materials, Sabina Dhakal

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The first part of this dissertation focuses on the design, synthesis, and characterization of a thermally robust S =1/2 phototetrazolinyl monoradical and the development of synthetic methodologies for a high-spin (S = 1) phototetrazolinyl diradical. Electrochemical studies of the phototetrazolium cation (precursor to the monoradical) revealed a remarkably narrow electrochemical band gap (Ecell ≈ 0.82 V), suggesting promising electrical conductivity. Thermal analysis of the monoradical demonstrated excellent stability, with the onset of decomposition at 232 °C. Building on the excellent thermal stability and promising properties for electrical conductivity of the monoradical, we developed two condensation-based synthetic routes that provide viable …

Investigating Preservice Elementary Teachers’ Stem Teaching Self-Efficacy And Goal Orientation, Derek Scott Cox

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

In the context of teacher education, self-efficacy and goal orientation can have an impact on one’s motivation, desire to improve, and maintaining a growth mindset in teachers. This study utilized an explanatory mixed methods design to investigate how and why preservice elementary science, technology, engineering, and mathematics (STEM) teachers’ confidence and views on competency changes after completing an array of courses built around integrated STEM.

Sixty-five preservice teachers participated in the pre- post-test quantitative data collection to determine changes in integrated STEM self-efficacy and achievement goal orientations (mastery-approach, mastery-avoidance, performance-approach, performance-avoidance). Data collection measures include Self-Efficacy for Teaching Integrated STEM …

Large-Scale Experimental Study On The Impact Of Air Cleaning & Ventilation On Indoor Air Quality And Student Illness-Related Absenteeism, Daud Nosham

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This large-scale randomized experimental field study investigated the effect of portable air purifiers (PAPs) on classroom’s air quality and student’s illness related absenteeism. PAPs were installed in 317 classrooms from various school districts located in Eastern Nebraska. The classrooms were randomly assigned into four conditions: three treatment conditions (T1, T2 & T3) and one control condition (device without any filter). T1 was equipped with HEPA filters, T2 added Activated Carbon (AC) layer to HEPA, and T3 further integrated Germicidal Ultraviolet (GUV) alongside HEPA and AC filters. The measured variables were carbon dioxide (CO2), fine particles (PN2.5) and coarse particles (PNcoarse), …

Assessing Crop Rotation Productivity, Soil Health, And Fertility, And Economic Performance, To Enhance Semi-Arid High Plains Cropping Systems Sustainability, Ezequiel Maria Villamil

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Sustainable crop production in the semi-arid High Plains of Nebraska faces challenges from limited precipitation, high interannual variability, and economic risk. This dissertation investigates the long-term productivity, water use, soil health, and economic sustainability of dryland crop rotations, with emphasis on the role of pulses as alternatives to fallow.

Chapter 1 analyzes nearly three decades (1995–2023) of on-farm data from the High Plains Agricultural Laboratory (Sidney, Nebraska) to compare seven crop rotations, including wheat, corn, millet, sunflower, and field peas. Results showed that wheat–corn–fallow (W–C–F) consistently outperformed traditional wheat–fallow (W–F) and more intensified four-year rotations, providing the highest grain yields, …

Development Of Databases For Glycan Utilization And Anti-Prokaryotic Defense In Microbiomes, Yuchen Yan

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Microbiomes in humans, animals, plants, and other environments are necessary components of the biosphere for global nutrient cycling and maintaining ecosystem balance. In particular, the gut microbiome plays an essential role in modulating human health through glycan utilization. Carbohydrate-active enzymes (CAZymes), CAZyme gene clusters (CGCs), and polysaccharide utilization loci (PULs) are key genomic features that determine microbial capacities for carbohydrate metabolism. Meanwhile, the arms race between phages and prokaryotes has led to the evolution of various anti-prokaryotic immune systems (APIS) in phages to counteract prokaryotic immune systems (PIS).

As bioinformatics methods used to analyze microbiome information advance rapidly, there is …

Advancing Precision Agriculture Through The Application Of Remote Sensing Technologies In Plant Health Assessment, Thomas Wilbur Davis

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Monitoring plant health, including nutritional status assessment, is an important component of crop management decisions. This doctoral document addresses the use of agricultural remote sensing through focused investigations of plant disease detection and nutrient status assessment technologies that advance precision agriculture. The first chapter provides a comprehensive overview of remote sensing technologies in agriculture and examines current capabilities, limitations, and future directions. The final two chapters address studies that evaluated the virus-nitrogen interaction in wheat and compared methods to determine the nitrogen status of midseason field corn.

The first study evaluated the wheat streak mosaic virus (WSMV) × nitrogen interaction …