Digital Commons Network^™

Evaluating The Sna1 Gene Using Crispr Mediated Knockdown In Ewing Sarcoma Cells, Rosanna Jees, Sonia Cerrillo, Terry Jo Shackleford

Mechanisms of Disease

Ewing sarcoma is a pediatric cancer with limited therapeutic options and poor long-term survival. CRISPR–Cas9–mediated gene editing provides a powerful tool for investigating tumor molecular behavior and identifying potential therapeutic targets. In this study, we worked with CRISPR–Cas9 in Ewing sarcoma ES8 cells to evaluate the roles of the transcription factors SNAI1 and SNAI2, both implicated in epithelial–mesenchymal transition and cancer progression. Using guide RNAs targeting each gene, we assessed proliferation, apoptosis, migration, and long-term survival through Incucyte live-cell imaging and colony-formation assays. Knockout of SNAI1 resulted in decreased cell proliferation and reduced migratory capacity compared with controls, suggesting a …

How Cxcr4 Knockdown Affects Tumorigenic Properties In Es8 Cells, Gabriella Galdeano, Damon James, Terry Jo Shackleford

Mechanisms of Disease

C-X-C chemokine receptor type 4 (CXCR4) is a seven-transmembrane G-protein–coupled receptor (GPCR). When activated by its ligand SDF-1 (CXCL12), CXCR4 triggers signaling pathways that promote cell survival, proliferation, angiogenesis, and chemotaxis. Many cancers exploit the CXCR4/SDF-1 axis to support tumor growth and metastasis. Ewing sarcoma (ES8) is an aggressive pediatric bone and soft-tissue cancer, and elevated CXCR4 signaling has been linked to increased migration and metastatic behavior. ES8 cells, a well-established ES8 cell line, provided a model to study how CXCR4contributes to tumor progression. CRISPR-Cas9 genome editing was used to knock down CXCR4 in ES8 cells, delivered through lipid-based transfection. …

Microplastic Exposure Alters Tubulin Leading To Alterations In Insulin Secretion By Pancreatic Beta Cells, Anakaren Garcia

Honors Program Theses and Research Projects

Abstract

Diabetes mellitus is a metabolic disorder characterized by elevated blood glucose levels due to impaired insulin secretion or diminished cellular response to insulin (resistance). Pancreatic β-cells depend on a well-organized cytoskeleton, particularly microtubules composed of α- and β-tubulin heterodimers, to transport insulin granules to the plasma membrane during glucose-stimulated insulin secretion (GSIS). Disruption of microtubule organization can impair granule trafficking and reduce insulin release. However, emerging environmental toxicants such as microplastics that interfere with β-cell cytoskeletal function remains largely unexplored.

Microplastics (MPs) are small plastic particles (< 5 mm) that have become widespread environmental contaminants and can enter biological systems. Previous studies have shown that MPs can induce cellular stress and protein misfolding, but their effects on microtubules in pancreatic β-cells are not well characterized. This study investigates whether exposure to fluorescent polystyrene microplastics alters microtubule organization and insulin secretion in βTC-6 cells.

βTC-6 cells were treated with 10 µg/mL of fluorescent polystyrene microplastics for …

Ian Colburn, Ian Colburn, Katie Slack

Oral Histories of Farming along the Middle Rio Grande

No abstract provided.

A Sedimentary Source Of Rare Earth Elements In The North Atlantic, Rylee D. Lindner

Electronic Theses and Dissertations

Individually and as a series, the Rare Earth Elements (REEs) are powerful geochemical tracers of environmental processes. However, ambiguities in the oceanic budgets of the REEs hamper their application. Growing evidence suggests a sedimentary benthic flux via the pore water is the major source of REEs to the ocean, with extrapolated estimates of this flux being equivalent in magnitude to that “missing” from modern budgets. However, existing pore water REE data are available from only a few locations globally with no representative dataset from the open North Atlantic. This data scarcity limits our ability to constrain flux estimates and understand …

Healthcare Access, Economic Stability, And Food Security And Inflammatory Bowel Disease In Hispanic Adults Living In The United States, Jennifer Rodriguez

Walden Dissertations and Doctoral Studies

Hispanic adults in the United States experience disparities in chronic disease detection and outcomes, including inflammatory bowel disease (IBD). Social determinants of health (SDoH), including economic stability, healthcare access, and food security, play a crucial role in shaping health risks and diagnostic opportunities in this population. The purpose of this quantitative cross-sectional study was to examine the relationship between selected SDoH factors and self-reported IBD diagnosis among U.S. Hispanic adults. This study utilized survey data from the 2023 National Health Interview Survey (n = 29,457). Weighted descriptive statistics and logistic regression were used to analyze the relationships between variables while …

Midweek Memo, Georgia Southern University

Midweek Memo

• Applaud
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• Around the Nest

Expressing Diversity And Anxiety Among Graduate Psychology Students, Paola G. Garcia Betancourt

Walden Dissertations and Doctoral Studies

This quantitative study examined the relationship between motivational factors that contribute to challenges in expressing diversity and the levels of anxiety experienced by graduate psychology students when discussing racial topics. The study was grounded in critical race theory, which posits that racism is embedded within social and institutional systems and influences interpersonal and educational dynamics. The purpose of the study was to determine whether motivational factors, as measured by the Color-Blind Racial Attitudes Scale, predicted anxiety levels, as measured by the Patient-Reported Outcomes Measurement Information System Emotional Distress Anxiety Short Form, and whether racial background moderated this relationship. Data were …

A Canadian Advanced Physiotherapist Practitioner Shared-Care Model In Pediatric Rheumatology Offers Safe And Quality Care In The Management Of Juvenile Idiopathic Arthritis-Comparing Key Performance Indicators With The Pr-Coin Registry., Julie Herrington, Patrick Clarkin, Jade Singleton, Karen Beattie, Sheetal S. Vora, Katelyn Banschbach, Catherine A. Bingham, Tania Cellucci, Danielle Fair, Mileka Gilbert, Beth Gottlieb, Julia G. Harris, Liane Heale, Tzielan Lee, Melissa L. Mannion, Edward J. Oberle, Nancy Pan, Jonathan Park, Mary Toth, Jennifer E. Weiss, Michelle Batthish, Pediatric Rheumatology Care And Outcomes Improvement Network

Manuscripts, Articles, Book Chapters and Other Papers

Background/Objectives: Canadian Advanced Physiotherapist Practitioner (APP) roles have existed for over 25 years in pediatric rheumatology. The APP can manage many common pediatric rheumatic conditions most often in Shared-Care Models (SCMs) with pediatric rheumatologists (PRs). The quality of care children receive in an APP SCM compared to traditional care is unknown. The Pediatric Rheumatology Care and Outcomes Improvement Network (PR-COIN) tracks quality measures as Key Performance Indicators (KPIs) in juvenile idiopathic arthritis (JIA) care. This study aimed to analyze the frequency of KPIs documented in a pediatric rheumatology APP SCM from a single center and compare to PR-COIN's performance …

Community Water Fluoridation And Oral Health: Proposal & Systematic Review, Kristina Lauranzano

Integrative Learning Experience (ILE) Theses

Fluoridation of community drinking water systems has been a staple of preventative dental care in the United States since the mid 20^th century. In recent years, there has been increased public discourse pertaining to the safety and efficacy of Community Water Fluoridation (CWF). The aim of the proposed study is to identify if there is an association between the CWF status (never fluoridated, currently fluoridated, or formerly fluoridated) and oral health status of a community in New England. This study will be a retrospective cross-sectional study design using NHANES Oral Health Status Survey data from the 2007-2008 and 2017-2018 …

The Impact Of Discontinuation And Non-Publishing Of Osteoporosis Clinical Trials, Daxton Kennington, Andrew Simonsen, Whitney Shae, Catherine L. Satterwhite

Student Publications

Context: Osteoporosis is a prevalent chronic disease associated with fractures, reduced quality of life, and substantial healthcare costs. Randomized controlled trials (RCTs) are essential for developing effective treatments, but when trials are discontinued or unpublished, valuable data are lost. This results in unnecessary costs and exposes thousands of participants to interventions without contributing to clinical care.

Objectives: This study aims to evaluate the rates and characteristics of discontinuation and nonpublication among US-registered phase 3 and 4 RCTs investigating osteoporosis therapies from 2000 to 2022.

Methods: Phase 3 and 4 osteoporosis-related RCTs were identified through ClinicalTrials.gov. Trial completion and publication status …

High School Science Experience For Students With Cognitive Disabilities: A Pilot Study, Cassandra Wychers

Honors Theses

Although education has become more adaptive, inclusive of all students who learn differently including for people with cognitive disabilities, closer examination may reveal that high school science classrooms are still challenging learning spaces. Science is an important part of the school curriculum as it teaches students about engaging in the world around them while also encouraging skills involving evidence, inquiries, and critical thinking. All students are entitled to learn science in their classrooms even if they have a cognitive disability (Apanasionok, et al. 2019). Although there are many complexities to defining disability in the world and in education, this study …

Sanando La Herida Abierta: The Open-Borders Debate, Latinx Feminist Voices, And Spiritual Activism, Amely Logan

Honors Program Theses and Research Projects

This thesis addresses the current discourse in philosophy—specifically, immigration ethics—that harms migrants, would-be migrants, and Latinx people in general. It proposes that these harms can be healed by taking seriously the contributions of Latinx feminists and paying special attention to spiritual activism. The thesis first introduces the open-borders debate in the philosophical literature and the harmful language and framing that affects migrant autonomy within the debate, specifically by looking at the work of Christopher Heath Wellman as a representative example. It reveals how, while border enforcement often results in literal mass casualties among immigrants, the language and framing around immigration …

Itgb1 Knockdown Alters Apoptosis And Proliferation In Ewing Sarcoma Cells, Aditi Kumar, Magdalena Saad, Terry J. Shackleford

Mechanisms of Disease

Integrin beta 1 (ITGB1) is an adhesion receptor that link cells to the extracellular matrix and regulates pathways involved in survival, proliferation, and migration. In order to understand its role in Ewing Sarcoma, we applied CRISPR-Cas9 with two guide RNAs to knock down ITGB1 in ES8 cells. We then assessed changes in cell growth, death, movement, and gene expression using Incucyte live-cell imaging, colony formation assays, wound healing assays, and qRT-PCR. qRT-PCR showed partial ITGB1 reduction, with ITGB1_gRNA2 producing the strongest decrease. ITGB1_gRNA2 also led to a small increase in caspase activity and a 20-30% increase in proliferation at the …

Effects Of Ptk2 Knockdown On Key Cellular Processes In Es8 Cells, Adrian Cruz, Emiliano Rueda, Dylan Andrews, Terry Jo Shackleford

Mechanisms of Disease

Ewing sarcoma is an aggressive form of pediatric cancer characterized by rapid growth and metastatic potential, with limited treatment options available. PTK2 is a key regulator of many pathways involved in tumor behavior, including integrin-mediated adhesion, survival, and migration. This research lab investigated how CRISPR-Cas9 knockdown of PTK2 can affect cellular processes in ES8 Ewing sarcoma cells. To assess the effectiveness of reducing PTK2 expression, we utilized live-cell imaging, wound-healing assays, measurements of caspase activity, colony formation, and qRT-PCR. Our results showed that incomplete knockdown decreased cell proliferation and colony formation, while also increasing apoptotic activity. Additionally, migratory ability was …

Message From The President, Georgia Southern University

Messages from the President

No abstract provided.

Retrospective Review Of Metastatic Hormone Receptor-Positive Inflammatory Breast Cancer Patients Reveals Poor Responses To Cyclin Dependent Kinase 4/6 Inhibition, Azadeh Nasrazadani, Rebecca S Tidwell, Megumi Kai, Bora Lim, Vicente Valero, Debu Tripathy, Sadia Saleem, Bisrat G Debeb, Anthony Lucci, Wendy A Woodward, Rachel M Layman

Faculty, Staff and Student Publications

Background: Patients with inflammatory breast cancer (IBC) have aggressive biology and relatively inferior responses to standard-of-care (SOC) therapies. Understanding the efficacy of SOC therapies in IBC is critical to optimize outcomes. Our objective was to assess the progression-free survival (PFS) of metastatic hormone receptor-positive HER2-negative/low (HR+HER2-) IBC patients treated with CDK4/6 inhibitors (CDKIs) and hormonal therapy (HT).

Methods: Data from 58 IBC patients with metastatic HR + /HER2- IBC from a single institution were reviewed. The medians (95% confidence intervals) of overall survival (OS), PFS, and time on treatment (ToT) from the time of CDKI initiation were reported via the …

Electrochemical Detection Of Aβ42 And Aβ40 At Attomolar Scale Via Optimised Antibody Loading On Pyr-Nhs-Functionalised 3d Graphene Foam Electrodes, Muhsin Dogan, Sophia Nazir, David Jenkins, Yinghui Wei, Genhua Pan

School of Engineering, Computing and Mathematics

Alzheimer’s Disease (AD) is one of the most commonly seen neurodegenerative disorders, where early detection of its biomarkers is crucial for effective management. Conventional diagnostic methods are often expensive, time-consuming, and highly complex, which highlights an urgent need for point-of-care biosensing technology. In this work, we developed assays on three-dimensional (3D) graphene foam electrodes by functionalising them with a 1-Pyrenebutyric acid N-hydroxysuccinimide ester (Pyr-NHS) to enable effective antibody immobilisation for the detection of amyloid beta peptides (Aβ42 and Aβ40), key biomarkers for AD. Pyr-NHS linkers were used for stable functionalisation, followed by binding with Aβ42 and Aβ40 antibodies, and then …

Seed Amplification Of Msa Alpha-Synuclein Aggregates Preserves The Biological And Structural Properties Of Brain-Derived Aggregates, Fei Wang, Victor Banerjee, Carla Barria, Santiago Ramirez, Tyler Allison, Damian Gorski, Haley Evans, Quynh Nguyen, Danielle Harrison, Rabab Al-Lahham, Nicole De Gregorio Carbonell, Michelle Pinho, Sanne Kaalund, Jonas Folke, Susana Aznar, Luis Concha-Marambio, Mohd Ishtikhar, Venkata Kps Mallampalli, Sandra Pritzkow, Mohammad Shahnawaz, Matthew L Baker, Irina Serysheva, Claudio Soto

Faculty, Staff and Student Publications

Parkinson's disease (PD), Dementia with Lewy bodies (DLB), and multiple system atrophy (MSA), are characterized by the misfolding and aggregation of alpha-synuclein (αSyn). Compelling evidence showed that αSyn aggregates exist as distinct conformational strains in different synucleinopathies. Recently, we reported that the αSyn Seed Amplification Assay (αSyn-SAA) can amplify and distinguish αSyn strains from PD and MSA. In this study, we investigate whether MSA-seeded, SAA-amplified αSyn fibrils retain the biological and structural properties of the αSyn seeds present in MSA brains. We study the biological activities of both brain-derived and SAA-amplified αSyn aggregates using an αSyn "biosensor" cell model and …

Predicting, Culturing, And Characterizing Beneficial And Detrimental Strains Associated With Plant Phenotypes., Anastasiia Onyshchenko

Arts & Sciences Graduate Student Theses and Dissertations

Modern agriculture’s reliance on chemical fertilizers has greatly increased crop yields but has also contributed to soil degradation, environmental pollution, and unsustainable resource use. Plant growth–promoting rhizobacteria offer a sustainable alternative, enhancing plant growth through mechanisms such as nitrogen fixation, phosphate solubilization, siderophore production, and phytohormone modulation, while also protecting plants against pathogens and abiotic stresses. However, soil microbial communities are highly diverse and structurally complex, making the isolation of individual beneficial strains challenging. Traditional approaches rely on selective media and labor-intensive phenotypic screening, which often capture only a small fraction of soil diversity. To overcome these limitations, we combined …

A Conversation With Paolo Lugari, Founder Of Las Gaviotas, Colombia, Jonathan Andelson

Communal Societies

No abstract provided.

Virtuous Mother Or Wanton Woman?: Gendered Rhetoric In Shaker And Anti-Shaker Publications In The Early Republic, Lindy Cummings

Communal Societies

No abstract provided.

Review Of Issachar Bates: A Shaker's Journey, Mary Ann Haagen

Communal Societies

No abstract provided.

Elucidating The Mechanism Of Neprilysin-Regulated Triple-Negative Breast Cancer Invasion, Kai Lam, Ellie Griner

Student Scholar Symposium

Triple-negative breast cancer (TNBC) is a highly invasive and difficult-to-target form of cancer. It is characterized by a lack of estrogen receptors, progesterone receptors, and human epidermal growth factor 2 receptors, making current targeted therapies ineffective and leaving chemotherapy as the primary treatment option. Previous studies in this lab have linked reduced expression of neprilysin (NEP), a membrane-bound protease that cleaves and inactivates mitogenic peptides, to a highly invasive phenotype in TNBC. Our data indicates that while NEP promotes phosphoinositol 3-kinase (PI3K) signaling, it simultaneously inhibits cellular invasion. Here, we begin to explain a possible mechanism behind the anti-invasive role …

Δ-Valerobetaine As A Novel Inhibitor Of Breast Cancer Cell Migration: Hinders Cellular Migration Of Mcf-7 Cells Through Reducing Atp Concentrations And Fak Activation, Braxton Ivie, Lincoln Brown, Bryson Lovorn

Student Scholar Symposium

δ-valerobetaine as a Novel Inhibitor of Breast Cancer Cell Migration: Hinders Cellular Migration of MCF-7 Cells Through Reducing ATP Concentrations and FAK Activation

Lincoln Brown, Braxton Ivie, Bryson Lovorn, Joshua Owens

Based on female breast cancer diagnoses from 2014-2020, the CDC reports that the 5-year relative survival rate for local and regional breast cancers was 98.9% and 86.3%, respectively, while the survival rate for metastasized cancers was reportedly 32.4%. These discrepancies in survival highlight an increased mortality rate when cancer metastasizes. Interestingly, aggressive tumors have been shown to metabolize fats at higher rates than low-metastatic tumors. Recently, our group has …

Exploring The Role Of Faecalibacterium Prausnitzii Metabolites In Modulating Fatty Acid Metabolism And Protecting Hepg2 Cells During Induced Steatosis, Morgan Martin, Emerald Cole

Student Scholar Symposium

The obesity epidemic has risen to astronomical numbers with over 650 million adults being classified as obese (BMI≥30). Obesity is of critical concern as excess body fat storage is a comorbidity to various diseases including type-2 diabetes, cardiovascular disease, and cancer. Although obesity has classically been attributed to genetics and unhealthy lifestyle choices, there is a growing interest in other factors affecting obesity, including the gut microbiome. For example, mice lacking a microbiome are resistant to weight gain even when placed on a high-calorie diet. It can then be concluded that bacteria and the metabolites they produce can dramatically affect …

Creating A Decision-Making Curriculum For Students With And Without Disabilities In Grades P-12, Gina Riley, Elizabeth Klein, Alfonso Perez, Imani Irving Perez, Adriana D. Nathan

Excelsior: Leadership in Teaching and Learning

No abstract provided.

Empowering Future Teacher Leaders Through Peer Mentoring, Stephanie M. Banks

Excelsior: Leadership in Teaching and Learning

No abstract provided.

Preservice Teachers And Creativity: Beliefs, Experiences, And Intentions For Future Practice, Joanna M. Weld

Excelsior: Leadership in Teaching and Learning

No abstract provided.

Purification Of Water By Plant Extracts And Green Biosynthesized Agnp Of Syzygium Aromaticum, Seham Abdel-Shafi, Mostafa I. Abdelglil, Saida Amer, Sobhy El Sawy Elsilk, Khaled Ali, Mohamed F Ghaly

Al-Farahidi Biomedical Journal

The drinking water quality must meet both the WHO guidelines and Egyptian standards. This ensures public health and the suitability of water for different uses. The purification of water by biosynthesized silver nanoparticles (AgNPs) Syzygium aromaticum (Clove) is an eco-friendly approach where the clove extract acts as a reducing and stabilizing agent to create AgNPs through a “green synthesis” method. The AgNPs, when combined with the extract, show antimicrobial activities and kill the pathogenic bacteria in water. The water pollution include Staphylococcus, Pseudomonas, Salmonella, Acinetobacter, Flavobacterium, Clostridium, Vibrio and E. coli. In vitro experiments showed inhibition of Staphylococcus spp. …