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Identifying The Molecular Cause Of Extreme Endoplasmic Reticulum Dilation In Pediatric Osteosarcoma And Its Relationship To The Disease, Rachael Wood

Theses and Dissertations (ETD)

Pediatric osteosarcoma tumors are characterized by an unusual abundance of grossly dilated endoplasmic reticulum and an immense genomic instability that has complicated identifying new effective molecular therapeutic targets. Here we report a novel molecular signature that encompasses the majority of 108 patient tumor samples, PDXs and osteosarcoma cell lines. These tumors exhibit reduced expression of four critical COPII vesicle proteins that has resulted in the accumulation of procollagen-I protein within ‘hallmark’ dilated ER. Using CRISPR activation technology, increased expression of only SAR1A and SEC24D to physiologically normal levels was sufficient to restore both collagen-I secretion and resolve dilated ER morphology …

Characterizing And Overcoming Resistance To Aminomethylspectinomycins In Gram-Negative Bacteria, Nisha Das

Theses and Dissertations (ETD)

Spectinomycin (SPC) is a broad-spectrum aminocyclitol antibiotic. Its use in agriculture has led to widespread resistance in enteric bacteria, necessitating the development of more effective analogs. Aminomethyl spectinomycins (amSPC) are modified spectinomycins with increased potency against many bacterial species. These species include Legionella pneumophila, which harbors a chromosomally encoded aminoglycoside modifying enzyme (AME). In this study, we follow up on this observation and examine the extent to which the amSPCs are substrates for AMEs through adenylation (ANTs) and phosphorylation (APH). APH(9)-Ia and ANT(3")(9) were expressed in E. coli BL21(DE3) and purified using the Ni-affinity chromatography. The ability of AMEs to …

Examination Of Antiviral Resistance In Venezuelan Equine Encephalitis Virus, Jasper Lee

Theses and Dissertations (ETD)

Venezuelan equine encephalitis virus (VEEV) is a New World Alphavirus that causes Venezuelan equine encephalitis (VEE), which is characterized by a febrile illness that can progress to neurological disease and death. While no major outbreaks of VEE have occurred since 1995, VEEV is a virus of concern as, in addition to its spread through mosquitos, it can be aerosolized and used as a bioweapon. Unfortunately, there are currently no FDA-approved vaccines or antivirals against VEEV. Efforts have been made to discover small molecules with an inhibitory effect on VEEV, but the potential for emergence of antiviral resistance to these compounds …

The Effect Of Cancer Cachexia Progression On The Feeding Regulation Of Skeletal Muscle Protein Turnover, Brittany R. Franch

Theses and Dissertations (ETD)

Cancer cachexia is defined as the unintentional loss of skeletal muscle mass with or without fat loss that cannot be reversed by conventional nutritional support. Cachexia occurs in ~20% of cancer patients. More specifically, 50% of lung cancer patients, the most common cancer worldwide, develop cachexia. Cachexia occurs most often in lung and gastrointestinal cancers, whereas breast and prostate have the lowest rate of cachexia. Cancer-induced cachexia disrupts skeletal muscle protein turnover (decreasing protein synthesis and increasing protein degradation). Skeletal muscle’s capacity for protein synthesis is highly sensitive to local and systemic stimuli that are controlled by mTORC1 and AMPK …

Vaccine Hesitancy - When Emotions Trump Reason, Lawrence C. Scharmann

Department of Teaching, Learning, and Teacher Education: Faculty Publications

Isn’t getting a vaccine a decision of choice or personal liberty? Yes, but only if personal choices don’t create health risks for other citizens. We no longer permit individuals to smoke in public spaces in which second-hand smoke can harm the health of others. In the case of COVID-19, at best, individuals whose choice it is not to be vaccinated slow progress toward herd immunity. At worst, if enough individuals choose not to vaccinate, this pandemic continues unabated, enabling variants of the original virus to emerge – variants that are often of increasing virulence. Fear of ingredients, however, is but …

In Vitro Investigation Of Tumor Selective Piperidones As Therapeutic Agents Against Leukemia Cancer Cells, Lisett Contreras

Open Access Theses & Dissertations

Cancer is a continuous global health issue. It is the second leading cause of death behind heart disease. Disparities across the emergence of cancer and resulting fatalities raise the importance of researching the disease. Treatments are available for certain types of cancers. However, these are typically accompanied by residual problems including side effects and the possibility for relapse. Some treatments attack all cells, leading to unwarranted side effects that make the possibility of living a comfortable life nearly impossible. Other treatments are specific to certain genetic alterations, making them only useful for a small percentage of patients. Not one treatment …

Interval Constraint-Solving Strategies For Solving Dynamical Systems, Angel Fernando Garcia Contreras

Open Access Theses & Dissertations

There are abundant phenomena that humans can describe through mathematical models. Dynamical systems are one such type of model, describing the behavior of phenomena that change over time. For example, a scientist can measure and analyze an insect's wing parameters and movements to create a dynamical system of that behavior. We can then use this model in different applications, such as creating a nano vehicle with insect-like propulsion.

For many real life problems, there exist analytical solutions. These can represent a full description of the state of a dynamical system at any moment in continuous time. However, in most practical …

Comprehensive Study Of Human Pathogenic Trypanosomatids: From Chemotherapies To Disease Ecology, Felipe Rodriguez

Open Access Theses & Dissertations

The vector-borne diseases Leishmaniasis and Chagas disease, caused by the kinetoplastid parasites Leishmania spp. and Trypanosoma cruzi respectively, are among the most important parasitic diseases in the group of neglected tropical diseases. Even though the two diseases are endemic to the Americas, they affect millions of people worldwide. Leishmaniasis and Chagas disease cause a great array of symptoms and some of them can be fatal if left undiagnosed and untreated. Current treatment regimens are becoming less effective, vaccines are still not available, and diagnosis needs to be improved. Vector control has been responsible for a decrease of diseases in endemic …

Utilizing Computational Approaches To Study Tubulin- Tubulin Electrostatic Interactions, Tolulope Ayodeji Ale

Open Access Theses & Dissertations

Microtubules play significant roles in transporting cargos within the cell, as well as the cell cycle. They are essentially the highway for the transportation of cell organelles. Understanding the interactions among microtubule components is crucial for the treatment of neurodegenerative diseases and cancer. In this study, we utilize a series of computational approaches to study the electrostatic interactions at the binding interfaces of interacting tubulin monomers. Our study revealed that the electrostatic attractive interactions in the αβ tubulin dimer is the strongest while the interaction between two αβ tubulin dimers in the longitude direction is the weakest. The result identified …

Computational Study Of Grain Structure Evolution In Cdte/Cds Via Molecular Dynamics, Sharmin Abdullah

Open Access Theses & Dissertations

Grain structure analysis plays an important role in the identification of grain boundary characteristics, which can affect the efficiency of Cadmium Telluride/Cadmium Sulfide (CdTe/CdS) solar cells since they can act as recombination centers for carriers. Computer simulations such as molecular dynamics (MD) can be a very convenient and cost- effective method of investigating the growth evolution and grain structure of materials. The recently reported and experimentally validated MD simulated growth of polycrystalline CdTe/CdS films shows that these materials mostly consist of zinc blende (ZB) and wurtzite (WZ) structures, along with highly disordered atoms. However, little information about the semiconductor compound …

Examination Of The Association Between C - Reactive Protein(Crp) And Covid-19 Infection Severity And Length Of Hospitalization, Abdulahi Aremu Ayanwale

Open Access Theses & Dissertations

Chronic stress can lead to many systemic complications and low-grade systemic inflammation including increased levels of inflammatory cytokines, such as C-reactive protein (CRP). CRP is a marker of systemic inflammation and is associated with depression and perceived stress. Elevations can result in ineffective immune responses, thereby increasing the risk of complications and mortality from infections. Recent evidence suggests that uncontrolled inflammatory responses associated with COVID 19 are a major determinant of disease severity.  The COVID 19 is transmitted from one person to another through droplets from coughing, sneezing, talking, touching droplets on surfaces and contamination by hand-to-mouth routes. The mechanism …

An Investigation Of Upper Extremity Motor Impairments In Children Diagnosed With Autism Spectrum Disorder: Motor Planning Vs Motor Execution, Patrick Anthony Cereceres

Open Access Theses & Dissertations

Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder typically diagnosedwhen a child displays social and communication impairments, repetitive behaviors, and rhythmic movements like hand flapping. This disorder is unique because of the high variability of symptoms displayed between each individual who is diagnosed. Because of this, no neurological or physiological test exists to diagnose a child with ASD. Current diagnostic tests are various forms of interviews or observations which an experienced clinician must administer. While these tests have been instrumental in diagnosing children with ASD, many go undiagnosed, which can be detrimental as they transition into adulthood. Current literature indicates …

Marine Phytoplankton Functional Types Exhibit Diverse Responses To Thermal Change, S. I. Anderson, A. D. Barton, S. Clayton, S. Dutkiewicz, T. A. Rynearson

Graduate School of Oceanography Faculty Publications

Marine phytoplankton generate half of global primary production, making them essential to ecosystem functioning and biogeochemical cycling. Though phytoplankton are phylogenetically diverse, studies rarely designate unique thermal traits to different taxa, resulting in coarse representations of phytoplankton thermal responses. Here we assessed phytoplankton functional responses to temperature using empirically derived thermal growth rates from four principal contributors to marine productivity: diatoms, dinoflagellates, cyanobacteria, and coccolithophores. Using modeled sea surface temperatures for 1950–1970 and 2080–2100, we explored potential alterations to each group’s growth rates and geographical distribution under a future climate change scenario. Contrary to the commonly applied Eppley formulation, our …

Development Of Metaheuristic Algorithms For The Efficient Allocation Of Power Flow Control Devices, Eduardo Jose Castillo Fatule

Open Access Theses & Dissertations

Modern energy grids have become extremely complex systems, requiring more variable and active flow control. As a remedy to this, Distributed Flexible AC Transmission Systems (D-FACTS) are cost-efficient devices used to mitigate power flow congestion and integrate renewable energies. The objective of this research is then to propose an efficient multiple objective evolutionary algorithm to solve a stochastic model for D-FACTS allocation, which aims to optimize various objectives related to cost, grid health, and environmental impacts. The model was implemented on a modified RTS-96 test system, and the results show that optimally allocating D-FACTS modules using the proposed model can …

Linking Plot And Landscape Level Phenology, Alejandra Flores

Open Access Theses & Dissertations

Plant phenology has become popularize since the 1990s to help understand the effects of climate change on vegetation, it is a way to study the patterns of leaf-out, flowering, and senescing of the life-cycle of the plant. The timing from start of season (SOS) to end-of-season (EOS) of plant leaf-out development has important implication on ecosystem productivity and carbon cycling across ecological scales from individual trees to whole regions. The need for plant phenology studies in dryland are highly anticipated, since they make up 40% of the terrestrial landscape and recently have shown the play an important role in the …

Molecular Mechanism Of Dna Bisintercalator Antibiotic Resistance, Priyanka Rajendra Gade

Open Access Theses & Dissertations

Class II UvrA is an ATP-binding cassette protein and a homolog of class I UvrA, the DNA lesion detection component of the universal prokaryotic nucleotide excision repair system. We show that Ecm16, a class II UvrA protein from Streptomyces lasalocidi, specifically recognizes DNA duplex that contains the DNA bisintercalator antibiotic echinomycin. Heterologous expression of Ecm16 in Escherichia coli renders host cells resistant to echinomycin. Interestingly, Ecm16’s antibiotic resistance activity does not require the DNA repair proteins UvrA/B/C/D, indicating that it operates independently of the cell’s nucleotide excision repair pathway. Unlike UvrA, Ecm16 uses the insertion domain to regulate its ATPase …

Geophysical Studies Of The Characteristics Of Fluvial And Desert Soils In Rio Grande Valley Of West Texas And Southern New Mexico, Aimee V. Garcia

Open Access Theses & Dissertations

Agriculture in arid lands, such as in the Rio Grande Valley in west Texas, relies on flood irrigation. However, flood irrigation can cause salt buildup and greenhouse gas emissions associated with pedogenic carbonate precipitation. The ability of irrigation to promote crop growth is influenced by geochemical and hydrological processes controlled, in part, by soil grain size, larger-scale soil structure, and mineralogy. This study investigated soils from 3 regions 1) A pecan orchard in the river valley of Tornillo, TX, with soil derived from fluvial deposits, undergoing flood irrigation every 2 to 3 weeks in spring through fall, 2) The El …

Student Attitudes Toward Science: Growth Trajectories And Thematic Analysis Of A Project-Based Internship, Robert Donavin Mccreary

Open Access Theses & Dissertations

In order to increase the number of students pursuing science-related careers, science educators and policymakers have advocated for improving student attitudes toward science (SAS) through inquiry-based instruction, such as project-based learning (PBL). While challenges exist in PBL, research suggests cogenerative dialogue (cogen) can ease the transition for students from traditional learning to PBL. In the present study, a secondary analysis was conducted to examine SAS growth trajectories from participants of the Work With A Scientist Program (WWASP) – a student-scientist internship which integrated cogen with PBL. Additionally, a thematic analysis was conducted to explore student attitudes toward cogen. The growth …

Leading In Gendered Spaces: Women Presidents' Perceptions Of Their Experiences In Community Colleges, Keri Moe

Open Access Theses & Dissertations

Gender inequality in leadership is an ongoing challenge found in higher education, including community colleges. Since women remain underrepresented in leadership positions at community colleges, specifically as college presidents, the purpose of this study was to document and better understand the experiences of women in these roles by focusing on three research questions:

• RQ 1: How do women presidents describe their experience in male-dominated community colleges?
• RQ 2: How do gender identity and gender expectations of women impact the behavior of women community college presidents?
• RQ 3: How do women presidents navigate their role within male-dominated community colleges?

Through the …

Goes-16 Level 2 Land Surface Temperature Product-Filling For Cloud Masked Data, Guadalupe Ortega

Open Access Theses & Dissertations

The GOES-16 satellite ground segment team develops different levels of environmental products and makes them readily available to the public. The level 2 Land Surface Temperature (LST) product aims to provide a remote measurement of the Earth’s surface temperature every hour. LST is of fundamental importance to many aspects of the geosciences, for example, to study the Urban Heat Islands (UHI) effect. The product however, does not provide values for pixels where the satellite imager’s view is obstructed by clouds, as determined by a cloud mask intermediate product. The objective of this thesis is to estimate LST in the cloud …

Racial Diversity Of Texas School Teachers, Julie Ann Olguin-Phillips

Electronic Theses and Dissertations

There is a growing gap between the racial demographics of public-school teachers and their students. Teachers with demographic backgrounds similar to a school’s student population are often successful, particularly in Title 1 schools with high proportions of minority students; however, teacher turnover is high. The students in Texas public schools are a growing minority-majority population. Changes in the student body racial composition and other school-related variables could influence teacher workforce demographics. The focus of discussion of this quantitative research study is to determine the factors that predict the racial diversity of teachers in Texas high schools. The research questions addressed …

Natural Regeneration Dynamics And Survival Influenced By Abiotic And Biotic Factors In A Bottomland Hardwood Forest, Lydia J. Voth Rurup

Electronic Theses and Dissertations

Riparian ecosystems are vital to the landscape, providing critical services including water filtration and purification, flood and erosion control, carbon sequestration, biodiversity support, and aesthetic value. Bottomland hardwood forests, however, are threatened by invasive species, land loss/conversion, inconsistent or absence of harvesting disturbances, and altered hydrological patterns, leading to reduced success of desired, native species. This research assessed regeneration dynamics and one-year survival in a seasonally-flooded bottomland hardwood forest at Boggy Slough Conservation Area in East Texas to identify abiotic and biotic factors important for successful establishment of native regeneration. Areas sampled included two that were previously treated with herbicide …

Effects Of Varying Warmup Stimuli On Running Economy, Connor Bigenho

Electronic Theses and Dissertations

Purpose: The purpose of this study was to determine the effects of two potentiating warm-up stimuli (weighted-vest strides and plyometrics) on running economy (RE) over short (5-10 minutes) and longer durations (30+ minutes) of exercise in trained distance runners. Methods: Five recreationally trained male runners were recruited to participate in this study. Each trial consisted of a 10-min self-paced warm-up on the treadmill followed by baseline RE test and baseline leg-stiffness. This was immediately followed by one of two intervention warmup protocols. The protocols consisted of either a series of plyometric movements, 6 x 10-sec strides with a …

Metaphors Of International Law, Harlan G. Cohen

Scholarly Works

This chapter explores international law in search of its hidden and not-so-hidden metaphors. In so doing, it discovers a world inhabited by states, where rules are mined or picked when ripe, where trade keeps boats forever afloat on rising tides. But is also unveils a world in which voices are silenced, inequality is ignored, and hands are washed of responsibility.

International law is built on metaphors. Metaphors provide a language to describe and convey the law’s operation, help international lawyers identify legal subjects and categorize situations in doctrinal categories, and provide normative justifications for the law. Exploring their operation at …

Role Of Sex Differences On Cancer Cachexia Progression And Fibrosis During Cancer Cachexia Development, Wesley Haynie

Graduate Theses and Dissertations

Cancer cachexia is a multifactorial wasting syndrome characterized by losses in bodymass >5% and occurs in approximately 80% of all cancer patients. Chronic inflammation and fibrosis play roles in cancer cachexia and a greater understanding of these contributing pathways to this pathology will pave the way for potential therapeutic avenues. While inflammation and fibrosis have been researched in various models of cancer cachexia, little to no studies have been performed in both sexes as most previous studies focus on males. PURPOSE The purpose of these studies is to investigate the role of fibrosis on cancer cachexia development as well as …

Exploiting The Final Frontier – Some Initial Thoughts On Regulating Humanity’S Relationships With Non-Terrestrial Life Forms, Rob Amos

Journal of Space Law

No abstract provided.

The Concept Of Long-Term Sustainability Of Outer Space Activities As An Emerging Source Of International Law, Jie Long, Wu Xie

Journal of Space Law

No abstract provided.

Small Grain Cereal Cover Crops For Late Fall Or Early Spring Grazing, Kallie J. Calus

Department of Animal Science: Dissertations, Theses, and Student Research

Beef cattle operations are confronted with early spring and late fall forage deficiencies. Producers in integrated crop and livestock systems can fill forage gaps using cover crops as a forage source in between cash crops.

A five-year study evaluating forage production, growing calf performance and economics of grazing an oats cover crop planted after corn silage (CS) and high moisture corn (HMC) corn harvests was conducted. The economic analysis accounted for costs of establishing and grazing the oats and the value of calf gain to determine fall grazing system profitability. Steers had greater average daily gain grazing oats after CS …

Characterizing Plasmids In Bacteria Species Relevant To Urinary Health, Cesar Montelongo Hernandez, Catherine Putonti, Alan J. Wolfe

Biology: Faculty Publications and Other Works

The urinary tract has a microbial community (the urinary microbiota or urobiota) that has been associated with human health. Whole genome sequencing of bacteria is a powerful tool, allowing investigation of the genomic content of the urobiota, also called the urinary microbiome (urobiome). Bacterial plasmids are a significant component of the urobiome yet are understudied. Because plasmids can be vectors and reservoirs for clinically relevant traits, they are important for urobiota dynamics and thus may have relevance to urinary health. In this project, we sought plasmids in 11 clinically relevant urinary species: Aerococcus urinae, Corynebacterium amycolatum, Enterococcus faecalis, Escherichia coli, …

Feeding A New Corn Milling Coproduct To Lactating Dairy Cattle: Examination Of Whole Animal Energy And Nitrogen Balance, Addison Lee Carroll

Department of Animal Science: Dissertations, Theses, and Student Research

New coproducts are continually developed in the ethanol production industry. With that, the new product originates from the dry milling process which is unlike the traditional process as nitrogenous based particles are concentrated from the residual fiber by sieving post fermentation. Although high protein coproducts have been available since the mid 2000’s. These new products must be evaluated for chemical composition so that they can be accurately described in feed libraries that are used in commercial ration formulation software. While chemical composition provides us an initial and useful description of a feed product, in vivo nitrogen and energy balance studies …