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Expression And Purification Of Phage T7 Ejection Proteins For Cryo-Em Analysis, Nicholas A. Swanson, Ravi K Lokareddy, Fenglin Li, Chun-Feng Hou, Mikhail Pavlenok, Michael Niederweis, Gino Cingolani

Department of Biochemistry and Molecular Biology Faculty Papers

Bacteriophages of the Podoviridae family densely package their genomes into precursor capsids alongside internal virion proteins called ejection proteins. In phage T7 these proteins (gp14, gp15, and gp16) are ejected into the host envelope forming a DNA-ejectosome for genome delivery. Here, we describe the purification and characterization of recombinant gp14, gp15, and gp16. This protocol was used for high-resolution cryo-EM structure analysis of the T7 periplasmic tunnel and can be adapted to study ejection proteins from other phages. For complete details on the use and execution of this protocol, please refer to Swanson et al.

Chemical And Biological Characterization Of The Anticancer Potency Of Salvia Fruticosa In A Model Of Human Malignant Melanoma, Sotiris Kyriakou, Venetia Tragkola, Michael Plioukas, Ioannis Anestopoulos, Paschalina S. Chatzopoulou, Eirini Sarrou, Dimitrios T. Trafalis, Maria V. Deligiorgi, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidi

School of Veterinary and Biomedical Sciences: Faculty Publications

Malignant melanoma is one of the most aggressive types of skin cancer with an increasing incidence worldwide. Thus, the development of innovative therapeutic approaches is of great importance. Salvia fruticosa (SF) is known for its anticancer properties and in this context, we aimed to investigate its potential anti-melanoma activity in an in vitro model of human malignant melanoma. Cytotoxicity was assessed through a colorimetric-based sulforhodamine-B (SRB) assay in primary malignant melanoma (A375), non-malignant melanoma epidermoid carcinoma (A431) and non-tumorigenic melanocyte neighbouring keratinocyte (HaCaT) cells. Among eight (8) different fractions of S. fruticosa extracts (SF1-SF8) tested, SF3 was found to possess …

Chemical Emissions From Cured And Uncured 3d-Printed Ventilator Patient Circuit Medical Parts, Jordan E. Krechmer, Brennan Phillips, Nicholas Chaloux, Russell Shomberg, Conner Daube, Gaurav Manchanda, Sam Murray, Alex Mccarthy, Rodrigo Fonseca, Jinen Thakkar, Brice Loose, Scott C. Herndon, John T. Jayne, Douglas R. Worsnop, Manjula R. Canagaratna

Graduate School of Oceanography Faculty Publications

Medical shortages during the COVID-19 pandemic saw numerous efforts to 3D print personal protective equipment and treatment supplies. There is, however, little research on the potential biocompatibility of 3D-printed parts using typical polymeric resins as pertaining to volatile organic compounds (VOCs), which have specific relevance for respiratory circuit equipment. Here, we measured VOCs emitted from freshly printed stereolithography (SLA) replacement medical parts using proton transfer reaction mass spectrometry and infrared differential absorption spectroscopy, and particulates using a scanning mobility particle sizer. We observed emission factors for individual VOCs ranging from ∼0.001 to ∼10 ng cm-3 min-1. Emissions were heavily dependent …

Distinct Circadian Assessments From Wearable Data Reveal Social Distancing Promoted Internal Desynchrony Between Circadian Markers, Yitong Huang, Caleb Mayer, Olivia J. Walch, Clark Bowman, Srijan Sen, Cathy Goldstein, Jonathan Tyler, Daniel B. Forger

Mathematics Sciences: Faculty Publications

Mobile measures of human circadian rhythms (CR) are needed in the age of chronotherapy. Two wearable measures of CR have recently been validated: one that uses heart rate to extract circadian rhythms that originate in the sinoatrial node of the heart, and another that uses activity to predict the laboratory gold standard and central circadian pacemaker marker, dim light melatonin onset (DLMO). We first find that the heart rate markers of normal real-world individuals align with laboratory DLMO measurements when we account for heart rate phase error. Next, we expand upon previous work that has examined sleep patterns or chronotypes …

Best Friends Forever And Family Ties: Continuity And Change In Closeness With Parents And Friends Among Australian Adolescents, Mckell A. Jorgensen-Wells

Theses and Dissertations

During adolescence, the need for social connection increases. Yet, fostering emotional closeness in relationships becomes more complex, as the need for autonomy also increases and social environments must adapt to become conducive to these seemingly competing needs. This complexity necessitates more research on what happens to close relationships during adolescence, so parents, scholars, and practitioners are better equipped to help individuals navigate the unique social atmosphere of adolescence. The current study draws upon multi-level modeling techniques to estimate growth models of Australian adolescents' closeness to parents and closeness to friends from ages 12-17 and examine predictors of these trajectories. Findings …

College Of Education News, Georgia Southern University

College of Education: News & Publications (2003-2023)

• Health and physical education major makes calls on his future as a coach with support from National Guard and Army ROTC
• Teacher development program on Georgia Southern’s Armstrong Campus offers mentorships for male students from diverse backgrounds
• Georgia Southern joins Girl Scouts in STEM education

Space And Species Interactions In Welfare Estimates For Invasive Species Policy, Stephanie Brockmann, Hongyan Zhang, Doran M. Mason, Edward S. Rutherford

Faculty Publications

Aquatic invasive species (AIS) can cause catastrophic damages to lake ecosystems. Bigheaded carp are one such species that pose a current threat to Lake Michigan. Bigheaded carp are expected to have spatially differentiated impacts on other aquatic species in the metapopulation. Policymakers must decide how much to invest in mitigation or conservation policies, if at all, by understanding how invasions impact social welfare or social wellbeing. Estimates of social welfare implications, however, may be biased if important interactions between species and space are overly simplified or aggregated out of the model. In this analysis, a bioeconomic model that links an …

Quick - Release End Effector Tool Interface, Shane Farritor, Thomas Frederick

Department of Mechanical and Materials Engineering: Faculty Publications

The various embodiments herein relate to a coupling apparatus for a medical device having a coupler body, a cavity defined in the coupler body, a rotatable drive component disposed within the cavity and having at least two pin receiving openings, and an actuable locking ring disposed around the cavity.

Animating The U.S. War Crimes Act, Beth Van Schaack

International Law Studies

All war crimes are challenging to prosecute. Typical reasons include the technicality of some constitutive elements, the difficulties of amassing sufficient evidence, the vagaries of unreliable or unavailable witnesses, and the often-impenetrable khaki wall of silence. Adding to these challenges, the United States has erected a number of idiosyncratic structural barriers in the way in which it has incorporated the prohibitions against war crimes into its domestic legal frameworks, both military and civilian. This article addresses problems with the U.S. federal war crimes statute and proposes reforms that would (1) better conform to U.S. obligations under the Geneva Conventions and …

Smoking And Violence: An Exploratory Study Within A Maximum Security Forensic Institution, Yvonne Lapp Cryns

Theses

Nicotine addiction among the mentally ill occurs at a high rate and aggressive behavior frequently occurs in forensic psychiatric facilities. Few studies focused on psychiatric forensic inpatients and their behavioral response to nicotine withdrawal. This study was an analysis of the association between nicotine and aggressive acts, reported in Special Incident Reports, before and after a smoking ban at a forensic hospital. The findings indicate a notable increase in aggressive acts in the week following implementation of a smoking ban with a parallel rise in use of seclusion and restraint. A significant decrease in self harm and a notable increase …

Hplc/Msn Profiling And Healing Activity Of A Muco-Adhesive Formula Of Salvadora Persica Against Acetic Acid-Induced Oral Ulcer In Rats, Nahla Ayoub, Nadia Badr, Saeed S. Ai-Ghamdi, Safaa Alsanosi, Abdullah R. Alzahrani, Ashraf B. Abdel-Naim, Khaled Nematallah, Noha Swilam

Pharmacy

Salvadora persica L. (S. persica, Siwak) is an ethnic plant that is widely used for improving oral hygiene. ThisstudyaimedtoprovideaphytochemicalprofilingofS.persica ethylacetate fraction (SPEAF)and to evaluate the healing activity of a muco-adhesive formula of the fraction against acetic acid-induced oral ulcers in rats. HPLC-ESI-QTOF-MS-MS analysis of SPEAF resulted in the tentative identification of 56 metabolites containing fatty acids (23%), urea derivatives (10.5%) and sulphur compounds (10%), in addition to several amides, polyphenols and organic acids (6.5%, 5% and 2%, respectively). For the first time, 19 compounds were identified from S. persica. In vitro and in vivo experiments indicated that the extract is …

Student Senate Meeting Minutes November 16, 2021, Student Senate-Winona State University

Student Senate Meeting Minutes

This document is the official Winona State University Student Senate Meeting Minutes for November 16, 2021.

Prevalence And Intensity Of Infection Of The Parasitic Copepod Salmincola Californiensis On Rainbow Trout In Birch Creek, Idaho, Andrew D. Suchomel, Eric J. Billman

Western North American Naturalist

Salmincola californiensis is a parasitic copepod that infects salmonid fishes in the genus Oncorhynchus. Our objective was to determine the level of infection of S. californiensis in Rainbow Trout in Birch Creek, Idaho. We sampled Rainbow Trout at 5 sites on Birch Creek during October 2015 and May 2016. Mean intensity of infection was greater in May 2016 (x̄ = 1.7) than it was in October 2015 (x̄ = 2.9; F_{1, 69} = 5.535, P = 0.021). Intensity of infection ranged from 1 to 10 copepods in October 2015 and 1 to 12 copepods in May …

The First Troglobitic Scorpions From Hispaniola, Greater Antilles: Two New Species Of Cazierius Francke, 1978 (Scorpiones: Diplocentridae), Rolando Teruel, Solanlly Carrero Jiménez, Gabriel De Los Santos

Euscorpius

Two new species of the Greater Antillean endemic scorpion genus Cazierius Francke, 1978, are herein described from the island of Hispaniola. They were found inside isolate limestone caves in the Dominican Republic and are fully troglomorphic, thus, apparently being strict troglobites that represent the first ever documented for this genus. The present additions raise the number of Hispaniolan species of both Cazierius and the family Diplocentridae Karsch, 1880, to five and ten, respectively.

Blue Crab In The New York Area As An Indicator For Climate Change, Adelle Molina

Sustainability Seminar Series

In the northeast US, winters are getting warmer, shorter, and milder due to climate change. It is hypothesized that populations of some species near their northern range limit may experience elevated population growth rates owing to lower mortality in winter, higher growth rates in summer, and the synergistic relationship between these physiological processes. Blue crabs (Callinectes sapidus) in New York estuaries are close to their northern range edge and are potentially climate “winners” in this region. To provide advice to managers, we monitored blue crab populations in a coastal, lagoonal estuary and used a combined modeling approach to simulate the …

Race And Reasonable Suspicion, Ric Simmons

Florida Law Review

The current political moment requires society to rethink the ways that race impacts policing. Many of the solutions will be political in nature, but legal reform is necessary as well. Law enforcement officers have a long history of considering a suspect’s race when conducting criminal investigations. The civil rights movement and the progressive criminal justice decisions of the Warren Court mitigated the explicit use of race as a factor, but there is ample evidence that many modern police officers still openly or implicitly use race to guide their investigative decisions.

This Article examines and critiques how courts have historically analyzed …

Expanding Lgbt, Marie-Amélie George

Florida Law Review

In many circles, “LGBT” is an antiquated acronym that excludes many of the individuals that the movement is supposed to serve. “LGBTQ,” “LGBTQIA,” and other variations of the acronym have become ever more pervasive as nonbinary, intersex, and asexual individuals have become increasingly visible. The LGBT initials that once signaled solidarity and intersection are now appearing limited because they highlight only certain subgroups.

Although many movement organizations have adopted a more expansive formulation of LGBT, national legal rights organizations have limited their agendas to LGBT issues. Until recently, they devoted the bulk of their efforts to gay and lesbian concerns …

Male Same-Sex "Horseplay": The Epicenter Of Sexual Harassment?, Kimberly D. Bailey

Florida Law Review

In Oncale v. Sundowner Offshore Services, Inc., the U.S. Supreme Court recognized same-sex sexual harassment as a cognizable claim of sex discrimination under Title VII of the Civil Rights Act of 1964. At the time, many scholars found this recognition to be significant and important, but some also argued that the Court provided an incomplete analysis regarding the meaning of discrimination “because of sex.” Specifically, some scholars argue that the Court’s opinion reinforces the sexual desire paradigm in the analysis of sexual harassment cases. Building upon this critique, this Article focuses specifically on the harassment of men who generally …

American Sign Language For Everyone: Considerations For Universal Design And Youth Identity, Samuel J. Supalla, Anita Small, Joanne S. Cripps

Society for American Sign Language Journal

No abstract provided.

American Sign Language And The Academy: The Little Language That Could, Brenda Jo Brueggemann

Society for American Sign Language Journal

No abstract provided.

Origins Of The American Deaf-World: Assimilating And Differentiating Societies And Their Relation To Genetic Patterning, Harlan Lane, Richard Pillard, Mary French

Society for American Sign Language Journal

No abstract provided.

Insights From Two Decades Of P-12 Engineering Education Research, Cary I. Sneider, Mihir K. Ravel

Journal of Pre-College Engineering Education Research (J-PEER)

The 21st century has seen a growing movement in the United States towards the adoption of engineering and technology as a complement to science education. Motivated by this shift, this article offers insights into engineering education for grades P-12, based on a landscape review of 263 empirical research studies spanning the two decades from January 2000 to June 2021. These insights are organized around three core themes: (1) students’ understandings, skills, and attitudes about engineering and technology; (2) effective methods of P-12 engineering education; and (3) benefits of P-12 engineering education. The insights are captured in the form of evidence-based …

Development And Validation Of A Survey Instrument Targeting Teachers’ Perceptions Of The Scope Of Engineering, Jacob Pleasants

Journal of Pre-College Engineering Education Research (J-PEER)

Around the world, pre-college teachers are increasingly being called upon to address engineering, often as part of science instruction. Teachers are typically tasked with engaging students in authentic engineering design activities to promote students’ development of design practices while deepening their knowledge of relevant science concepts. Planning and implementing engineering experiences that authentically reflect the field require that teachers hold accurate perceptions of what engineering is, how it works, and how it is related to yet distinct from science. As teacher education and research efforts in this area grow, there is a need for high-quality instruments that elicit key aspects …

Advances In Rhizobial Research – Progress Priorities In Temperate Areas, Angela Sessitsch

IGC Proceedings (1977-2023)

Rhizobia are well known for their capacity to establish a symbiosis with legumes. During this symbiosis the bacteria inhabit root nodules where they reduce atmospheric nitrogen and make it available to the plant. Biological nitrogen fixation (BNF) is an important source of nitrogen and the various legume crops and pasture species often fix as much as 200-300 kg nitrogen per hectare (Peoples et al., 1995). Globally, symbiotic nitrogen fixation has been estimated to amount to at least 70 million metric tons of nitrogen per year (Brockwell et al., 1995). Furthermore, in many cases nitrogen fertilizers are not efficiently used by …

The Legume – Rhizobia Symbiosis. Does It Vary For The Tropics Relative To The Mediterranean Basin?, J. G. Howieson, A. Mcinnes

IGC Proceedings (1977-2023)

Symbiotic N fixation from legumes is one of the most important biological processes on the planet. It currently provides the majority of the N requirement in agriculture, yet will have to double if cereal crop production is to meet world demand by 2020 (Kennedy and Cocking 1997). To effectively harness the value of biological N fixation from legumes we need to more fully understand G² x E; where G refers to the genotypes of both the legume (G_l) and its microsymbiont (rhizobia; G_r), and E refers to the edaphic environment in which the symbiosis is …

Advances In Forage Legumes: Shrub Legumes, M. H. Shelton

IGC Proceedings (1977-2023)

Forage tree legumes benefit agriculture through their multi-purpose contributions to livestock feeding, to productivity of farming systems, and to protection of the environment.

Of the several hundred forage tree legume species regarded as having potential for forage only c. twenty are in common use. These are listed together with their agroecological range and tolerance of adverse environments including acid soils, cool temperatures, low rainfall, poor drainage and high salinity. The value of many species to ruminants is limited due to low nutritive value arising from low protein availability and low acceptability caused principally by high condensed tannin content. Only 10 …

Alfalfa, J. H. Bouton

IGC Proceedings (1977-2023)

Cultivated alfalfa or lucerne (Medicago sativa spp. sativa) is the most important forage legume in the world with approximately 32 million hectares cultivated mainly in the temperate regions of both the northern and southern hemispheres. Intensive research has been conducted on the management and physiology of the crop and a major seed industry has developed world wide. Since insect and disease pests are numerous in alfalfa, development of cultivars with the proper fall dormancy and a broad genetic base for pest resistance has been pursued in order to provide increased adaptation, persistence, and yield. There has been a …

Carbon And Nitrogen Isotopic Discrimination In Alfalfa For High Yield And Quality Forage, W. S. Oliveira, P. P. A. Oliveira, M. Corsi, S. M. Tsai

IGC Proceedings (1977-2023)

Biological nitrogen fixation and assimilation of CO₂ can be indirectly monitored in alfalfa (Medicago sativa L.) through the variation of the stable N and C isotopes in the plant (Δ values). Such measurements result in a simple, non-destructive and reliable method for identification of alfalfa cultivars of high yield and nutritional quality. A program for selection of 12 alfalfa cultivars under field and controlled conditions (lysimeters) included determination of biomass and N accumulation. Under controlled conditions yields ranged from 1.77 to 2.47 ton/ha/month (Pioneer 5312 and WL-605) and N2-fixed varied from 77.43 to 84.01% for total N (Brazilian …

Power Reclaimed And Given: Female Passivity And Agency In Harris, Brand, And Smith, Annika Northrop

Journal of Interdisciplinary Undergraduate Research

The paper “Power Reclaimed and Given: Female Passivity and Agency in Harris, Brand, and Smith” explores representations of women in art in Claire Harris’ “After Image,” Dionne Brand’s “Blues Spiritual for Mammy Prater,” and Ali Smith’s How to be both. Harris’ poem depicts a woman, who is the subject of a male photographer’s photos and who has been objectified and relegated to passivity by the photographer, in the active process of reclaiming the power to create her own identity. Brand’s poem shows Mammy Prater as a woman who, although formerly subjugated and abused, has already reclaimed that power; in …

Effects Of Freshwater Input On Nutrient Loading, Toxic Cyanobacteria Bloom Development And Cyanotoxin Production In Lake Pontchartrain Estuary, Louisiana, Callie L. Snow

LSU Master's Theses

Estuaries in the northern Gulf of Mexico (GoM) are undergoing profound alterations due to changes in climate and implementation of coastal restoration activities and these may lead to the increasing frequency of harmful algal blooms (HABs). The introduction of large volumes of nutrient-rich, fresh Mississippi River water into nutrient-poor Lake Pontchartrain Estuary is known to substantially change the chemistry and ecology of the estuary, and warm temperatures paired with the persistent nutrient loading into the estuary supports the proliferation of potentially toxic cyanobacteria blooms (CyanoHABs). Throughout 2019, a long-term opening of the Bonnet Carré Spillway (BCS), initially from February to …