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Investigating The Potential Role Of Horse Flies (Diptera: Tabanidae) In The Mechanical Transmission Of Anaplasma Marginale And Theileria Orientalis To Cattle In Northwest Arkansas, Catherine Harrison Jan 2026

Investigating The Potential Role Of Horse Flies (Diptera: Tabanidae) In The Mechanical Transmission Of Anaplasma Marginale And Theileria Orientalis To Cattle In Northwest Arkansas, Catherine Harrison

2026 Research Poster Competition

The cattle industry in Arkansas plays a significant role in the state’s agricultural economy; however, two emerging infectious diseases pose a threat to cattle health, producer livelihood, and animal welfare. The two diseases, anaplasmosis and theileriosis, are caused by organisms that enter the host’s bloodstream and infect red blood cells, leading to destruction of said cells. The shared symptoms of both diseases are fever, weakness, anemia (low red blood cell count), reduced productivity, weight loss, and possibly death. An important difference between symptoms is that, unlike anaplasmosis, theileriosis can cause abortion in cattle. Both diseases are known to spread through …


Examining Life Course Trends In Dental Tissues Measurements In Members Of The Cayo Santiago Macaque Colony, Tyler Moore Jan 2026

Examining Life Course Trends In Dental Tissues Measurements In Members Of The Cayo Santiago Macaque Colony, Tyler Moore

2026 Research Poster Competition

Dental tissue dynamics in primates are largely understood: as enamel wears with tooth use, tertiary dentin is laid down in response to cumulative (micro)traumas, compressing the internal pulp chamber and root canals. However, our knowledge of the rate and pattern of change in tissue quantities across the life course of primates remains incomplete, particularly in well-documented free-ranging populations. This study examined age- and sex-related variation in dental tissue surface area (SA) and volume (V) in a skeletal sample of rhesus macaques (Macaca mulatta) from the Cayo Santiago colony (Puerto Rico), a population with known life history data. Forty-six individuals were …


Uav-Based Sensor Integration For In-Situ Lake Water Quality Monitoring, Alec Campbell Jan 2026

Uav-Based Sensor Integration For In-Situ Lake Water Quality Monitoring, Alec Campbell

2026 Research Poster Competition

Current water quality assessment methods rely heavily on manual sampling and laboratory analysis, which are time-consuming and difficult to perform in remote or hazardous environments. In surface water monitoring, spatial and temporal resolution is often constrained by labor, access, and logistical limitations of manual sampling programs. This research aims to develop a drone-based system for real-time, in situ lake water quality monitoring. To address these challenges, the project focuses on integrating multiple water quality sensors, including oxidation-reduction potential (ORP), pH, electrical conductivity (EC), turbidity, and a multispectral spectrometer, into a compact, UAV-compatible design capable of collecting spatially referenced data. Methods: …


Functional Characterization Of The Reniform Nematode Effector Protein Rr6640 In Soybean Parasitism, Noah Solomon Jan 2026

Functional Characterization Of The Reniform Nematode Effector Protein Rr6640 In Soybean Parasitism, Noah Solomon

2026 Research Poster Competition

Reniform nematode (Rotylenchulus reniformis, RN) is a major plant-pest affecting key crops in Arkansas such as cotton and soybean, causing economic losses exceeding $757 million in the U.S. over the past decade. While some genetic resistance exists in cotton, soybean remains highly susceptible, exacerbating RN populations in crop rotations. To address this gap, this research investigates the mechanisms of nematode infection focusing on effectors, the specialized proteins secreted by the parasite to manipulate host cell biology and suppress plant immunity. This study characterizes Rr6640, a putative effector gene identified as an ortholog to a virulence factor in the Beet Cyst …


Testing The Effects Of Mechanical Stimulation On Mesenchymal Stem Cell Cultures, Ella Collis Jan 2026

Testing The Effects Of Mechanical Stimulation On Mesenchymal Stem Cell Cultures, Ella Collis

2026 Research Poster Competition

Mesenchymal stem cells (MSCs) are a promising treatment option for a variety of diseases due to their ability to migrate to far-off sites, modulate inflammatory immune responses, secrete factors that aid in tissue healing, and their capacity to become several cell types. These cells can be found and isolated from various body tissues including bone marrow and adipose tissue, even so the number of cells obtained from these tissues is minimal compared to the amounts needed for their transplantation in cell therapy treatments. Therefore, their culture in plastic dishes is required to increase the number of cells and obtain the …


Effectiveness Of Occupational Recommendations In Maintaining Cognitive Function Under Hot-Dry And Warm-Humid Conditions, Zane Garner Jan 2026

Effectiveness Of Occupational Recommendations In Maintaining Cognitive Function Under Hot-Dry And Warm-Humid Conditions, Zane Garner

2026 Research Poster Competition

National Institute for Occupational Safety and Health (NIOSH) recommendations dictate heat safety practices in occupations throughout the United States. Currently, the effectiveness of these recommendations is unknown. Likewise, there is a lack of consensus regarding the effect of combined heat and exercise stress on cognitive function. PURPOSE: To examine the effectiveness of NIOSH heat safety recommendations in maintaining cognitive function after moderate work in the heat, alongside whether the type of heat has any effect. METHODS: A counter balanced, repeated measures, randomized controlled trial was conducted to evaluate differences in cognitive function in two distinct environments. Participants completed two experimental …


Developing A Cpp-Conjugated Cas9 Library For Efficient Non-Viral Delivery To The Dmd Gene, Amie Donathan Jan 2026

Developing A Cpp-Conjugated Cas9 Library For Efficient Non-Viral Delivery To The Dmd Gene, Amie Donathan

2026 Research Poster Competition

Duchenne muscular dystrophy, or DMD, is a genetic neuromuscular disorder caused by the mutation of the DMD gene, resulting in alterations in the functionality or production of dystrophin, a protein essential to maintain muscle fiber integrity and strength. This mutation leads to progressive muscle degeneration, causing motor loss, cardiopulmonary failure, and ultimately, premature death. Gene therapies using CRISPR aim to replace the mutated gene to provide long-term correction. Although this has promise, CRISPR-based therapies use a viral vector to deliver, which presents notable challenges, such as immune problems and liver toxicity. A non-viral delivery method is a viable approach, but …


Macroinvertebrate Taxonomic And Functional Diversity In The Spokane River Watershed, Kaitlin L. Abell Jan 2026

Macroinvertebrate Taxonomic And Functional Diversity In The Spokane River Watershed, Kaitlin L. Abell

EWU Masters Thesis Collection

Changes in land use, such as agricultural expansion, have impacted stream systems by modifying discharge, substrate diversity, channel morphology, and nutrient inputs. The Spokane River Watershed is heavily impacted by human activity, including urban and agricultural development. Rehabilitation efforts are being organized to reintroduce Salmonidae species in the Spokane River Watershed. The first step of this reintroduction is to perform habitat studies to inform allocation of restoration efforts. As part of this effort, I performed benthic macroinvertebrate surveys throughout the watershed.

The objective of my research was to use the macroinvertebrate community as a biological indicator to determine which habitat …


A Bayesian Late-Fusion Supportability Framework For Rare-Disease Severity Prediction In Glut1 Deficiency Syndrome, Jordan M. Rodriguez Jan 2026

A Bayesian Late-Fusion Supportability Framework For Rare-Disease Severity Prediction In Glut1 Deficiency Syndrome, Jordan M. Rodriguez

Mathematics Dissertations

Glucose transporter type 1 deficiency syndrome (GLUT1-DS) is a rare neurometabolic disorder with heterogeneous neurological and developmental severity. Because patient-level severity is not observed as a single validated outcome, this dissertation develops a Bayesian late-fusion supportability framework for constructing and predicting an ordered latent severity phenotype from clinical, genetic, and EEG-derived evidence. The primary target was constructed in a larger clinical cohort using age-5 symptom burden and learning cognition, then assigned to an aligned multimodal prediction cohort. Target-defining variables were excluded from supervised predictors, and models were evaluated using patient-exclusive cross-validation with training-fold preprocessing and fold-wise EEG PCA.

The primary …


Genome-Wide Gene-Sleep Interaction Study Identifies Novel Lipid Loci In 732,564 Participants, Raymond Noordam, Wenyi Wang, Pavithra Nagarajan, Heming Wang, Michael R Brown, Amy R Bentley, Qin Hui, Aldi T Kraja, John L Morrison, Jeffrey R O'Connel, Songmi Lee, Karen Schwander, Traci M Bartz, Lisa De Las Fuentes, Mary F Feitosa, Xiuqing Guo, Xu Hanfei, Sarah E Harris, Zhijie Huang, Mart Kals, Christophe Lefevre, Massimo Mangino, Yuri Milaneschi, Peter J Van Der Most, Natasha L Pacheco, Nicholette D Palmer, Varun Rao, Rainer Rauramaa, Quan Sun, Yasuharu Tabara, Dina Vojinovic, Yujie Wang, Stefan Weiss, Qian Yang, Wei Zhao, Wanying Zhu, Md Abu Yusuf Ansari, Hugues Aschard, Pramod Anugu, Themistocles L Assimes, John Attia, Laura D Baker, Christie Ballantyne, Lydia Bazzano, Eric Boerwinkle, Brain Cade, Hung-Hsin Chen, Wei Chen, Yii-Der Ida Chen, Zekai Chen, Kelly Cho, Ileana De Anda-Duran, Latchezar Dimitrov, Anh Do, Todd Edwards, Tariq Faquih, Aroon Hingorani, Susan P Fisher-Hoch, J Michael Gaziano, Sina A Gharib, Ayush Giri, Mohsen Ghanbari, Hans Jörgen Grabe, Mariaelisa Graff, C Charles Gu, Jiang He, Sami Heikkinen, James Hixson, Yuk-Lam Ho, Michelle M Hood, Serena C Houghton, Carrie A Karvonen-Gutierrez, Takahisa Kawaguchi, Tuomas O Kilpeläinen, Pirjo Komulainen, Henry J Lin, Gregorio V Linchangco, Annemarie I Luik, Jintao Ma, James B Meigs, Joseph B Mccormick, Cristina Menni, Ilja M Nolte, Jill M Norris, Lauren E Petty, Hannah G Polikowsky, Laura M Raffield, Stephen S Rich, Renata L Riha, Thomas C Russ, Edward A Ruiz-Narvaez, Colleen M Sitlani, Jennifer A Smith, Harold Snieder, Tamar Sofer, Botong Shen, Jingxian Tang, Kent D Taylor, Maris Teder-Laving, Rima Triatin, Michael Y Tsai, Henry Völzke, Kenneth E Westerman, Rui Xia, Jie Yao, Kristin L Young, Ruiyuan Zhang, Alan B Zonderman, Xiaofeng Zhu, Jennifer E Below, Simon R Cox, Michelle Evans, Myriam Fornage, Ervin R Fox, Nora Franceschini, Sioban D Harlow, Elizabeth Holliday, M Arfan Ikram, Tanika Kelly, Timo A Lakka, Deborah A Lawlor, Changwei Li, Ching-Ti Liu, Reedik Mägi, Alisa K Manning, Fumihiko Matsuda, Alanna C Morrison, Matthias Nauck, Kari E North, Brenda Wjh Penninx, Michael A Province, Bruce M Psaty, Jerome I Rotter, Tim D Spector, Lynne E Wagenknecht, Ko Willems Van Dijk, Lifelines Cohort Study, Million Veteran Program, Cashell E Jaquish, Peter Wf Wilson, Patricia A Peyser, Patricia B Munroe, Paul S De Vries, W James Gauderman, Yan V Sun, Han Chen, Clint L Miller, Thomas W Winkler, Dabeeru C Rao, Susan Redline, Diana Van Heemst Jan 2026

Genome-Wide Gene-Sleep Interaction Study Identifies Novel Lipid Loci In 732,564 Participants, Raymond Noordam, Wenyi Wang, Pavithra Nagarajan, Heming Wang, Michael R Brown, Amy R Bentley, Qin Hui, Aldi T Kraja, John L Morrison, Jeffrey R O'Connel, Songmi Lee, Karen Schwander, Traci M Bartz, Lisa De Las Fuentes, Mary F Feitosa, Xiuqing Guo, Xu Hanfei, Sarah E Harris, Zhijie Huang, Mart Kals, Christophe Lefevre, Massimo Mangino, Yuri Milaneschi, Peter J Van Der Most, Natasha L Pacheco, Nicholette D Palmer, Varun Rao, Rainer Rauramaa, Quan Sun, Yasuharu Tabara, Dina Vojinovic, Yujie Wang, Stefan Weiss, Qian Yang, Wei Zhao, Wanying Zhu, Md Abu Yusuf Ansari, Hugues Aschard, Pramod Anugu, Themistocles L Assimes, John Attia, Laura D Baker, Christie Ballantyne, Lydia Bazzano, Eric Boerwinkle, Brain Cade, Hung-Hsin Chen, Wei Chen, Yii-Der Ida Chen, Zekai Chen, Kelly Cho, Ileana De Anda-Duran, Latchezar Dimitrov, Anh Do, Todd Edwards, Tariq Faquih, Aroon Hingorani, Susan P Fisher-Hoch, J Michael Gaziano, Sina A Gharib, Ayush Giri, Mohsen Ghanbari, Hans Jörgen Grabe, Mariaelisa Graff, C Charles Gu, Jiang He, Sami Heikkinen, James Hixson, Yuk-Lam Ho, Michelle M Hood, Serena C Houghton, Carrie A Karvonen-Gutierrez, Takahisa Kawaguchi, Tuomas O Kilpeläinen, Pirjo Komulainen, Henry J Lin, Gregorio V Linchangco, Annemarie I Luik, Jintao Ma, James B Meigs, Joseph B Mccormick, Cristina Menni, Ilja M Nolte, Jill M Norris, Lauren E Petty, Hannah G Polikowsky, Laura M Raffield, Stephen S Rich, Renata L Riha, Thomas C Russ, Edward A Ruiz-Narvaez, Colleen M Sitlani, Jennifer A Smith, Harold Snieder, Tamar Sofer, Botong Shen, Jingxian Tang, Kent D Taylor, Maris Teder-Laving, Rima Triatin, Michael Y Tsai, Henry Völzke, Kenneth E Westerman, Rui Xia, Jie Yao, Kristin L Young, Ruiyuan Zhang, Alan B Zonderman, Xiaofeng Zhu, Jennifer E Below, Simon R Cox, Michelle Evans, Myriam Fornage, Ervin R Fox, Nora Franceschini, Sioban D Harlow, Elizabeth Holliday, M Arfan Ikram, Tanika Kelly, Timo A Lakka, Deborah A Lawlor, Changwei Li, Ching-Ti Liu, Reedik Mägi, Alisa K Manning, Fumihiko Matsuda, Alanna C Morrison, Matthias Nauck, Kari E North, Brenda Wjh Penninx, Michael A Province, Bruce M Psaty, Jerome I Rotter, Tim D Spector, Lynne E Wagenknecht, Ko Willems Van Dijk, Lifelines Cohort Study, Million Veteran Program, Cashell E Jaquish, Peter Wf Wilson, Patricia A Peyser, Patricia B Munroe, Paul S De Vries, W James Gauderman, Yan V Sun, Han Chen, Clint L Miller, Thomas W Winkler, Dabeeru C Rao, Susan Redline, Diana Van Heemst

Faculty, Staff and Student Publications

Background and aims: Deviations from the population mean in sleep duration have been associated with increased risk for developing dyslipidemia and atherosclerotic cardiovascular disease, but the mechanism of effect is poorly characterized. We performed large-scale genome-wide gene-sleep interaction analyses of lipid levels to identify genetic variants underpinning the biomolecular pathways of sleep-associated lipid disturbances and to suggest possible druggable targets.

Methods: We collected data from 55 cohorts with a combined sample size of 732,564 participants (87 % European ancestry) with data on lipid traits (high-density lipoprotein [HDL-c] and low-density lipoprotein [LDL-c] cholesterol and triglycerides [TG]). Short (STST) and long (LTST) …


Blood Flow Regulates Metabolism In Hematopoietic Development, Pamela L Wenzel Jan 2026

Blood Flow Regulates Metabolism In Hematopoietic Development, Pamela L Wenzel

Faculty, Staff and Student Publications

Blood flow modifies oxygen availability and biomechanical forces within the vasculature of the embryo as the hematopoietic system develops. The aorta-gonad-mesonephros (AGM) envelops the largest artery in the body and is a critical site for the emergence of hematopoietic stem cells (HSCs). Herein, I discuss the role of hypoxia-inducible factors (HIFs) and force as determinants of metabolism and fate determination. To address the effects of blood flow on hematopoietic development, I employ mouse embryo models and biomimetic culture. Real-time cell metabolic analyses show that oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) are altered by flow in cultures of …


Updates To Version 1.0 Of The Four‐Dimensional Ecology Education Framework Create Version 1.1., Christopher W. Beck, Loren B. Byrne, Jennifer Doherty, Amanda E. Sorensen, Et Al. Jan 2026

Updates To Version 1.0 Of The Four‐Dimensional Ecology Education Framework Create Version 1.1., Christopher W. Beck, Loren B. Byrne, Jennifer Doherty, Amanda E. Sorensen, Et Al.

Arts & Sciences Faculty Publications

Since its introduction in 2018, the Four-Dimensional Ecology Education (4DEE) Framework has advanced ecology education. Yet, ecology educators have had different understandings of aspects of the Framework. Here, we describe the updates to the original Framework that aim to provide more clarity. Specifically, we describe higher-level changes in how the Framework is presented on the 4DEE website, changes to some of the elements within the dimensions, mostly in the Human–Environment Interactions dimension, and perhaps most substantively, replacing the lists of subelements with more descriptive paragraphs that explain the intent of the elements. We hope that these updates will promote the …


Comparative Evaluation Of Hplc And Capillary Electrophoresis In Intestinal Permeability Of Oseltamivir Phosphate Via Single-Pass Intestinal Perfusion (Spip) Method, Senem Şanli, Gülpembe Halay, Mustafa Sinan Kaynak, Nurullah Şanlı Jan 2026

Comparative Evaluation Of Hplc And Capillary Electrophoresis In Intestinal Permeability Of Oseltamivir Phosphate Via Single-Pass Intestinal Perfusion (Spip) Method, Senem Şanli, Gülpembe Halay, Mustafa Sinan Kaynak, Nurullah Şanlı

Arts & Sciences Faculty Publications

Introduction: Oseltamivir phosphate (OP), a widely used antiviral prodrug for influenza, requires accurate Biopharmaceutics Classification System (BCS) classification to inform formulation and bioequivalence decisions. Despite its high solubility, the permeability characteristics of OP remain inadequately defined. Methods: This study evaluated the intestinal permeability of OP using the in situ Single-Pass Intestinal Perfusion (SPIP) model in male Sprague Dawley rats. Two validated analytical methods - High-Performance Liquid Chromatography (HPLC) and Capillary Zone Electrophoresis (CZE) were developed and compared. Metoprolol tartrate served as the high-permeability reference standard, and phenol red was used to monitor water flux. Permeability coefficients (Peff) were calculated after …


Differentiation Of Cell Populations In Saliva-Skin Mixtures Using Autofluorescence Profiling, Kara Fairfax Jan 2026

Differentiation Of Cell Populations In Saliva-Skin Mixtures Using Autofluorescence Profiling, Kara Fairfax

Master of Science in Forensic Science Directed Research Projects

While DNA profiling of sexual assault evidence can identify biological contributors, determining the tissue source of recovered profiles remains a significant challenge, particularly in cases of suspected oral assault involving mixtures of epithelial cells. The initial goal of this study was to identify non-genetic signatures for differentiating two types of epithelial cells relevant to oral assaults, saliva epithelial cells and epidermal skin cells. To develop tissue-specific signatures, reference cell populations from saliva and epidermal skin surfaces were collected from 20 donors and analyzed using full spectrum flow cytometry.

Results from single-source reference samples showed that saliva-derived particle populations exhibited higher …


Substance Use Behaviors And Treatment Engagement In Individuals With Severe Psychopathology With And Without Comorbid Obesity, Rachel M. Madera-Arnold Jan 2026

Substance Use Behaviors And Treatment Engagement In Individuals With Severe Psychopathology With And Without Comorbid Obesity, Rachel M. Madera-Arnold

Theses and Dissertations

Research has found significantly higher rates of both substance use and obesity among individuals with SMI compared to population samples which in turn can lead to worsening health outcomes. Despite this, there is limited research on the co-occurrence of obesity, substance use, and SMI and how these conditions may interact to affect patient outcomes. In addition, there are many gaps in the research regarding the relationship between substance use and body weight, appetite and/or eating behaviors. This study conducted a secondary analysis of the NSDUH 2021 survey that characterized substance use and disorders among individuals who had severe psychopathology, examined …


Family- And Cultural-Level Influences For Latine Adolescent Nicotine Use: A Qualitative Study Informing Prevention, Cindy M. Hernandez Castro Jan 2026

Family- And Cultural-Level Influences For Latine Adolescent Nicotine Use: A Qualitative Study Informing Prevention, Cindy M. Hernandez Castro

Theses and Dissertations

Early tobacco use among Latine adolescents represents a critical public health concern, with 23.8% reporting current use and exceeding rates among other racial/ethnic groups (Birdsey et al., 2023; Gaddy et al., 2022). This early initiation elevates risks for nicotine dependence and related morbidity in adulthood (Tsao et al., 2023). Guided by Structural Ecosystems Theory (Szapocznik & Coatsworth, 1999) and contemporary grounded theory analysis (Braun & Clarke, 2006; Charmaz, 2006; Noble & Mitchell, 2016), this study examined multilevel influences on nicotine-related attitudes among Latine youth, including: (1) familial factors (i.e., parental monitoring, caregiver-youth communication), (2) cultural dynamics (i.e., cultural values and …


Characterization Of Growth, Motility And C-Di-Gmp In Selenomonas Sputigena Atcc 35185, Margaret E J Stiker Jan 2026

Characterization Of Growth, Motility And C-Di-Gmp In Selenomonas Sputigena Atcc 35185, Margaret E J Stiker

Theses and Dissertations

Selenomonas sputigena is an obligate anaerobic, Gram-negative pathobiont that transitions from a low-abundance commensal bacteria to a structural component in dysbiotic oral biofilms associated with early childhood caries and periodontitis. Analysis of S. sputigena ATCC 35185 cultures optimized in TYGVS media revealed robust growth, characteristic crescent morphology with flagellar tuft motility, and strong monospecies biofilm formation comparable to Streptococcus mutans via crystal violet assays.

ELISA quantification demonstrated significantly elevated c-di-GMP levels in sessile biofilm fractions relative to motile planktonic cells (p=0.0009), establishing the secondary messenger’s correlation with the motile-sessile lifestyle switch. Recombinant expression and purification yielded PilZ-domain effector proteins, Selsp_1666 …


Environmental Monitoring In Shallow Lakes: Public Health Threats In Urban Waters, Kyleigh E. Johnson Jan 2026

Environmental Monitoring In Shallow Lakes: Public Health Threats In Urban Waters, Kyleigh E. Johnson

Theses and Dissertations

Lakes are an integral part of urban and suburban communities, providing ecological, recreational, and public health benefits. Many of these lakes are located in densely populated areas and are subject to urban influences on water quality. Algal toxins and Escherichia coli are two common recreational concerns. Algal toxins come from harmful algal blooms that are influenced by nutrient availability and temperature, while bacteria are often introduced through run-off and animal waste. Sampling every 3-4 weeks from May-October 2025 allowed for the assessment of seasonal patterns and site-specific differences across nine lakes in the Richmond area. The highest bacterial concentrations appeared …


Mathematical Models With Clinical Applications For Improving Health Outcomes, Helen Harris Jan 2026

Mathematical Models With Clinical Applications For Improving Health Outcomes, Helen Harris

Theses and Dissertations

In clinical settings, patients are exposed to many risks and stressors that could result in adverse health outcomes. Here we present mathematical models that seek to address these risks. First, we present a Markov Chain model to investigate the effect of medication reconciliation (MR) completion on patient health outcomes in the intensive care unit. Using this model, we simulate the annual incidence of adverse drug events (ADEs) for three different ADE rates. Based on the simulated results, we conduct a cost-benefit analysis for various levels of compliance to determine the financial implications of increasing MR completion depending on the baseline …


Absolute Quantification And Identification Of Rna From Rna-Lipid Nanoparticles Using High Resolution Mass Spectrometry, Jason C. Funderburk Jan 2026

Absolute Quantification And Identification Of Rna From Rna-Lipid Nanoparticles Using High Resolution Mass Spectrometry, Jason C. Funderburk

Theses and Dissertations

RNA therapeutics are a rising drug category with potential use for a range of conditions encompassing infectious diseases to therapies for cancer, diseases, and genetic disorders. RNA-lipid nanoparticles (RNA-LNPs) are the prominent delivery method for these therapeutics approved products include mRNA vaccines and polyneuropathy treatments. The emergency use authorizations and orphan drug status of current RNA-LNP drugs has allowed approval without finalization of the regulatory analytical procedures for quality monitoring. The objective of the study was to develop an LC-MS assay to simultaneously measure identity and concentration of two therapeutically relevant intact RNA constructs extracted from RNA-LNPs to enhance quality …


In Search Of The Soul Of Creativity: Authorship, Agency, And Meaning In Refractive Collaboration With Generative Ai, Fatima Nazir Jan 2026

In Search Of The Soul Of Creativity: Authorship, Agency, And Meaning In Refractive Collaboration With Generative Ai, Fatima Nazir

Theses and Dissertations

When images can be generated in seconds, what remains uniquely human in the act of creation? In Search of the Soul of Creativity: Authorship, Agency, and Meaning in Refractive Collaboration with Generative AI investigates where authorship, agency, and meaning reside within design practices mediated by generative AI. Rather than using AI to produce predictable results, this research deliberately relinquishes control to observe how generative systems interpret language through their own learned associations. Through a practice-led investigation across four generative models within the Higgsfield platform, user prompts were progressively shortened from full sentences to single words, revealing recurring patterns and cultural …


Magnetic Resonance Only Planning Validation Using Synthetic Ct By Mvision, Eric D. Brass Sr Jan 2026

Magnetic Resonance Only Planning Validation Using Synthetic Ct By Mvision, Eric D. Brass Sr

Theses and Dissertations

Problems: Adaptive radiotherapy requires clinically practical methods for responding to patient-anatomy changes that occur between the original planning images and the day of treatment delivery. Registering a previously acquired computed tomography scan to a newly acquired magnetic resonance image can introduce cross-modality uncertainty and may delay replanning. This thesis evaluates whether synthetic computed tomography generated from same-day magnetic resonance imaging can reduce registration-related uncertainty while supporting rapid dose recalculation for fraction-level adaptation.

Procedure: Magnetic resonance imaging DICOM data were exported from the ViewRay MRIdian treatment planning system and uploaded to MVision for synthetic computed tomography generation. The synthetic computed tomography …


The Ethereal Being That Is Me, And Maybe You, Hwiy Chang Jan 2026

The Ethereal Being That Is Me, And Maybe You, Hwiy Chang

Theses and Dissertations

The Ethereal Being That Is Me, and Maybe You is a 23-minute video collection of short stories accompanied by an installation at the Institute for Contemporary Art at Virginia Commonwealth University. This thesis explores the work’s creation as a visual and narrative meditation on self-transformation, drawing from personal experiences in therapy and philosophical texts including Carl Jung’s concept of the unconscious. The paper examines how each story represents a distinct stage of transformation—from recognizing patterns to confronting setbacks to reshaping internal dialogue—and how watercolor’s fluid, unpredictable nature serves as a visual metaphor for the process of change. The installation component …


Follow The Cyclists: Everyday Navigation Of Richmond's Bike Infrastructure, Emily E. Schreer Jan 2026

Follow The Cyclists: Everyday Navigation Of Richmond's Bike Infrastructure, Emily E. Schreer

Theses and Dissertations

This thesis explores the embodied experience of cycling in Richmond, emphasizing the importance of centering firsthand, lived experience in cycling research and urban planning. While traditional transportation planning can often myopically prioritize efficiency and safety metrics to evaluate infrastructure, this project argues that such approaches overlook essential experiential, sensory, and emotional dimensions of cycling that determine how infrastructure is perceived, used, and ultimately succeeds or fails.

Through an ethnographic approach combining field observation of cyclist behavior and mental mapping sessions with Richmond cyclists, this study positions people who bike in Richmond as expert knowledge holders. Participant contributions, combined with observations …


A Mutant Perfect, Amelia R. Baxter Jan 2026

A Mutant Perfect, Amelia R. Baxter

Theses and Dissertations

A Mutant Perfect examines how embodied knowledge can be made visible and transmissible through ceramic figures. The research asks what forms emerge when making is understood as a mode of thinking rather than a vehicle for pre-formed ideas, and how those forms might activate somatic recognition in a viewer. The primary method is coil-built ceramic sculpture. Color is applied in layered coats and removed by hand-sanding to expose varying strata beneath, producing a speckled, worn surface. In a second approach, pigment is built directly into the clay body through coil construction, making color structural rather than applied. Narrative prose and …


Characterization Of Microrna Expression In Neuron-Enriched Extracellular Vesicles During Early Life, Allison Molen Jan 2026

Characterization Of Microrna Expression In Neuron-Enriched Extracellular Vesicles During Early Life, Allison Molen

Theses and Dissertations

Extracellular vesicles (EVs) are membrane-bound sacs released by cells that carry molecular cargo reflective of their cell of origin. EVs enriched using L1 cell adhesion molecule (L1CAM) as a marker of putative neuronal origin can cross the blood–brain barrier and provide a minimally invasive window into brain-related processes. Exosomal microRNA (miRNA) regulate gene expression and have been implicated in psychiatric disorders, including major depressive disorder (MDD). However, measuring and analyzing miRNA from L1CAM-positive EVs presents substantial analytical challenges due to low abundance and sparse detection across samples.

Plasma samples from participants recruited through the Mid-Atlantic Twin Registry (MATR) were used …


Examining The Impact Of Trauma-Informed Mindfulness Meditation On Posttraumatic Growth Following Interpersonal Trauma: A Randomized Controlled Trial, Margaret Poandl Jan 2026

Examining The Impact Of Trauma-Informed Mindfulness Meditation On Posttraumatic Growth Following Interpersonal Trauma: A Randomized Controlled Trial, Margaret Poandl

Theses and Dissertations

Research on interventions designed to increase posttraumatic growth (PTG) among interpersonal trauma survivors is limited. The purpose of this study was to examine the impact of a six-week trauma-informed mindfulness meditation intervention on PTG. Additionally, I examined outcomes of posttraumatic stress (PTS) and loneliness, as well as analyzed the variables of dispositional mindfulness, hope, spirituality, and self-compassion to understand their potential role as mechanisms of change within the intervention on PTG. To answer these research questions, I conducted a randomized controlled trial study with 113 adults from a community sample who reported experiencing interpersonal trauma. Utilizing linear mixed modeling and …


Commissioning & Daily Qa Of Converted Linac For Electron Flash, Tomaj Javidtash Jan 2026

Commissioning & Daily Qa Of Converted Linac For Electron Flash, Tomaj Javidtash

Theses and Dissertations

Ultra-high dose rate (FLASH) radiotherapy has demonstrated promising normal tissue sparing in preclinical studies; however, the lack of standardized dosimetry, commissioning, and quality assurance (QA) methodologies remains a major barrier to its reproducible and safe implementation. In particular, conventional clinical commissioning and QA protocols are not directly applicable at FLASH dose rates due to challenges associated with detector response, dose-per-pulse effects, and beam control limitations.

This thesis presents the commissioning of a converted Varian 21EX linear accelerator for electron FLASH operation within the Division of Medical Physics at the Virginia Commonwealth University (VCU) Department of Radiation Oncology. Comprehensive beam characterization …


The Effects Of Overwash On Barrier Island Evolution And Building A Barrier Island Measure Of Resistance, Beth Thomas Jan 2026

The Effects Of Overwash On Barrier Island Evolution And Building A Barrier Island Measure Of Resistance, Beth Thomas

Theses and Dissertations

Barrier islands are critical for coastal communities, as they serve as a natural buffer against storm surge, waves, and the effects of rising sea levels, protecting life and property. These islands continuously evolve due to both normal and severe environmental conditions; global warming makes it increasingly difficult to predict the evolution of these islands due to increases in storm frequency and intensity. We present a cellular model of barrier island evolution consisting of biotic and abiotic processes including the effects of vegetation, wind, ocean currents, and gravity. The model is used to predict the future evolution of barrier islands off …


Machine Learning-Based Spatio-Temporal Modeling Of Climate Dynamics And Desertification In The Sahara–Sahel Region, Stephen M. Tivenan Jan 2026

Machine Learning-Based Spatio-Temporal Modeling Of Climate Dynamics And Desertification In The Sahara–Sahel Region, Stephen M. Tivenan

Theses and Dissertations

Arid climate classifications are threshold-dependent and easily interpretable mappings that are widely used in ecological, agricultural, and climate-related studies. These classifications inform scientific understanding, support policy and land management decisions, and provide an intuitive summary of environmental conditions. Despite their usefulness, traditional arid climate classifications often fail to quantify uncertainty, incorporate spatial context, or account for complex relationships among relevant environmental variables. Existing approaches to uncertainty assessment have largely relied on comparing classifications across multiple datasets or alternative formulas, but these methods generally overlook important spatial dependence and latent structure in the data.

This dissertation develops three machine learning-based statistical …