Digital Commons Network^™

Study Of Certain Biomarkers In Iraqi Female Patients With Breast Cancer, Abeer M. Hussain, Ali Hussein Mohammed Al-Khafaji, Alia Hussain Ali, Haider Latif Mohammed

Baghdad Science Journal

The prospective study has been designed to determine some biomarkers in Iraqi female patients withbreast cancer. The current study contained 30 patients whose tissue samples have been collected fromhospitals in Medical City in Baghdad after consent patients themselves and used immunohistochemicaltechnique to determine these markers. The results showed a significant correlation between ER and PR tissuemarkers (Sig = 0.000) and a significant correlation between cyclin E phenotype and cyclin E intensity (Sig =0.001).

Educating Asian American College Students On The Importance Of Community And Mentorship, Jeah Limbag

Capstone Projects and Master's Theses

Many college students may find it challenging to discover and create a supportive and consistent community or establish meaningful relationships in school. Asian American college students, in particular, have cultural values, experiences, and ideologies that can limit seeking mentors and establishing a social support network. The consequences of this could be increased feelings of loneliness and isolation, worse mental health, lower academic performance, lower attendance, and an increased likelihood of dropping out of school. To address these challenges, I have created an interactive presentation bringing awareness of the importance of mentorship and community to students in the Asian and Pacific …

Assigning Assessment Accommodations To English Learners: A Phenomenological Study, Heather May Villalobos Pavia

Dissertations

The English learner (EL) student population has grown steadily for the past 20 years. During this time, the use of standardized assessments has increased as well. Teacher understanding of assessment accommodations that best support ELs is low, despite the research that shows the unreliability of standardized achievement tests that measure the academic achievement of ELs. The purpose of this qualitative, phenomenological study was to examine how 10 teachers engage in the assessment accommodation selection process. Teachers were intentionally selected from intermediate elementary grades where the most native (primary/home) language accommodations are available on state assessments. Data were collected through surveys, …

Prominent Women Wind Conductors In Higher Education: Trials, Triumphs, And Recommendations For Improving Gender Equity In The Field, Jennifer Lee Grice

Dissertations

This study investigated the past and present status of gender equity in the wind band conducting profession in higher education. Eight of the most prominent women in wind band conducting were interviewed on specific questions regarding their trials, triumphs, and recommendations for upcoming women conductors in the field. Interviewees included Andrea Brown, Paula Crider, Tonya Mitchell-Spradlin, Emily Moss, Catherine Rand, Courtney Snyder, Emily Threinen, and LaToya Webb. Discussion topics included personal musical journeys, role models/mentors, equity in the profession, challenges faced in their careers, personal score study/rehearsal techniques, how to help future women in the field, and other pertinent information. …

Aryl Halides As Potential Components For O-Aryl-N-(9'-Acridinyl)-Hydroxylamines Antitumor Agents, Erin Munder

Master's Theses

Acridine derivatives such as m-amsacrine (m-AMSA) are known to intercalate deoxyribonucleic acid (DNA). Their action targets the growth, function, and replication of cancer cells. Because of this, they are primarily used as anticancer and antimalaria drugs. Unfortunately, due to the low half-life m-AMSA possesses because of the ease of hydrolysis of the carbon 9-nitrogen bond (C9-N bond), large concentrations of the drug are needed to be pharmaceutically effective. This results in a large concentration of toxic aniline byproducts. To reduce the rate of hydrolysis but still maintain the binding to DNA, a modification to the m-AMSA structure was made. A …

The Role Of The Erbb Signaling Pathway In Cardiovascular Progenitor Cell-Based Repair, Christopher Ramos

Electronic Theses, Projects, and Dissertations

Adult mammalian hearts lack self-renewal and proliferative capabilities necessary for cardiovascular regeneration. Current treatments using cardiac progenitor cells (CPCs) for cell-based repair do not restore cardiac function in patients who experience a myocardial infarction (MI). Our laboratory has been studying Islet-1+ neonatal CPCs as a promising candidate for cell-based repair due to their ability to significantly improve cardiac function after MI in sheep. The current study addresses the hypothesis that the ERBB pathway is linked to the Hippo-pathway to activate YAP1 by the involvement of an autocrine loop that upregulates neuregulin (NRG). In our sheep model of MI and cardiovascular …

A Small Molecule Produced By Lactobacillus Species Blocks Candida Albicans Filamentation By Inhibiting A Dyrk1-Family Kinase, Jessie Macalpine, Martin Daniel-Ivad, Zhongle Liu, Junko Yano, Nicole M. Revie, Robert T. Todd, Peter J. Stogios, Hiram Sanchez, Teresa R. O’Meara, Thomas A. Tompkins, Alexei Savchenko, Anna Selmecki, Amanda O. Veri, David R. Andes, Paul L. Fidel, Nicole Robbins, Justin Nodwell, Luke Whitesell, Leah E. Cowen

School of Dentistry Faculty Publications

The fungus Candida albicans is an opportunistic pathogen that can exploit imbalances in microbiome composition to invade its human host, causing pathologies ranging from vaginal candidiasis to fungal sepsis. Bacteria of the genus Lactobacillus are colonizers of human mucosa and can produce compounds with bioactivity against C. albicans. Here, we show that some Lactobacillus species produce a small molecule under laboratory conditions that blocks the C. albicans yeast-to-filament transition, an important virulence trait. It remains unexplored whether the compound is produced in the context of the human host. Bioassay-guided fractionation of Lactobacillus-conditioned medium linked this activity to 1-acetyl-β-carboline (1-ABC). We …

Trained Immunity Enhances The Immune Response And Maintains Microbiome Diversity In Aging And Sepsis, P. Spencer Gill

Electronic Theses and Dissertations

The global population is rapidly aging. It is estimated that over the next thirty years, the number of individuals >60 years of age will increase by over a billion, and the number of individuals over age 80 may increase by 300 million. As humans age, our immune system becomes progressively weaker through a process called immune senescence. This age-related decrease in immune function increases susceptibility to infection and chronic diseases. Sepsis is a leading cause of death worldwide. Over the past two decades, there has been an increased incidence of sepsis which is due, in part, to our aging population …

Correctional Career Pathways: A Jail Reentry Program Evaluation, Grace Gass

Electronic Theses and Dissertations

There are numerous types of reentry programs available to inmates to help avoid the cycle of recidivism. There is little research on the impacts of reentry programs in jail populations. This current study sought to provide more research in this area by evaluating a local jail reentry program’s effect on recidivism. A quasi-experimental design was used to estimate the program’s effectiveness by comparing the recidivism statistics of inmates that have participated in the reentry program to a control group of inmates that did not. Inmates in the control group were matched according to their gender, age, race, and current offense …

Lipidomic Analysis Of Various Developmental Stages Of Physcomitrium Patens, Deepshila Gautam

Electronic Theses and Dissertations

Lipids maintain fluidity of the cell membrane during the lifetime of all organisms. The moss Physcomitrium patens, an early land plant, enters reproductive phase under cold (15°C) conditions relative to its gametophytes (22°C). Thus, we hypothesized that their lipid content and composition would be distinct. Using ESI-MS/MS, we showed that the content and acyl composition of 11 lipid classes varied during development. Galactolipids were abundant in gametophytes but insignificant in sporophytes; among phospholipids, phosphatidylcholine was predominant in both phases. Although, sporophytes contained around five-fold less lipids than the gametophyte, their phosphatidic acid content, which accumulates during stress, was 18-fold …

Computational Analysis Of The Spin Trapping Properties Of Lipoic Acid And Dihydrolipoic Acid, Matthew Bonfield

Electronic Theses and Dissertations

While the spin trapping properties of thiols have been investigated through EPR analysis and kinetics studies, few groups have studied these properties using strictly computational methods. In particular, α-lipoic acid (ALA) and its reduced form, dihydrolipoic acid (DHLA), one of the strongest endogenously produced antioxidants, show potential for being effective, naturally occurring spin traps for the trapping of reactive oxygen species. This research covers electronic structure calculations of ALA, DHLA, and their corresponding hydroxyl radical spin adducts, performed at the cc-pVDZ/B3LYP/DFT level of theory. The effects on DHLA introduced by other radicals such as ·OOH, ·OCH₃, …

Genital Chlamydia Infection Is Influenced By The Female Sex Hormones Estrogen And Progesterone In Vivo, Amy Gail Gravitte

Electronic Theses and Dissertations

Chlamydia is the most common bacterial sexually transmitted infection in the United States and worldwide. It often goes unnoticed due to lack of symptoms and left untreated it can ascend the female genital tract to cause sequelae like pelvic inflammatory disease and irreversible tubal infertility. In reproductive-aged women, female sex hormones estrogen (E2) and progesterone (P4) concentrations fluctuate during the menstrual cycle and are influenced by hormonal contraceptives and hormone replacement therapy. E2 and P4 influence genital Chlamydia infection in women and mice, but these multifactorial interactions are not entirely mapped out. The complex interplay of E2 and P4 with …

Mesopredator Detection And Eastern Spotted Skunk Den Site Selection In North Carolina, Anna Siegfried

All Theses

Small carnivore assemblages have been a topic of interest for many years because of their potential top-down effect on communities. However, mesopredators are a challenge to investigate because of their small sizes and elusive behavior. Advances in technology, such as non-invasive trail cameras and smaller GPS tracking devices, have increased the success in monitoring these communities, and we are learning that some of these species’ populations have drastically declined, are currently declining, or the population status is unknown.

Adding an attractant to remote camera sites has become a popular method to increase detections of mesopredators. However, there is an ongoing …

Resolving Soft Error Susceptibilities Within Lossy Compressed Hpc Data, Dakota Kent Fulp

All Theses

Due to improvements in high-performance computing (HPC) systems, researchers have created powerful applications capable of solving previously intractable problems. While solving these problems, such applications create vast amounts of data, which stresses the I/O subsystem. Researchers use lossy compression to remedy this issue by reducing the data's size, but, as we demonstrate in this thesis, a single soft error leaves lossy compressed data unusable. Due to the high information content per bit ratio, lossy compressed data is sensitive to soft errors, which is an issue as soft errors have become commonplace on HPC systems. Yet, few works have sought to …

Effect Of Long And Short Chain Fatty Acids On Methane Yield And Microbial Community Composition In Anaerobic Co-Digestion, Emmaline Spier Camposano

All Theses

Fats, oils and greases are produced from a variety of industries, including animal rendering operations, vegetable oil processing plants and the hospitality industry. These hydrophobic, COD-rich compounds are known to cause issues in municipal wastewater collection and treatment systems. Anaerobic co-digestion has been shown to be an effective way to treat FOG and presents an opportunity to produce greater quantities of biogas. However, many microorganisms are sensitive to the degradation products of FOG, including long and short chain fatty acids. Two studies were performed to address potential inhibitory effects of both.

The first study co-digested various percentages of FOG with …

The Effects Of Audio/Visual Stimulation And Virtual Reality To Increase The Rate Of Retention In First Semester Freshmen Graphic Communications Students, Hanna Gibson

All Theses

In March of 2020, the world entered the COVID-19 pandemic, leaving educators to answer the question, "how do we teach in a virtual environment?". This was especially difficult for deeply rooted STEM programs such as Graphic Communications at Clemson University. This research aims to analyze multiple methods of virtual teaching for correlation between enhanced retention rates.

This thesis attempts to solve the problem facing global STEM educators when they are attempting to prepare material for students in a virtual environment. This is achieved by presenting them with how different methodologies impact the retention rate on freshmen level students enrolled in …

Does Naturalness Influence Positive Learning Outcomes During Environmental Education Field Trips?, Lydia Kiewra

All Theses

Environmental education (EE) field trip programs in the United States often take place in outdoor, natural settings. Such natural environments provide ample opportunity for hands-on and immersive learning to occur, which may allow for positive cognitive and moral development in children. However, recent research on the impact of more natural settings on youths’ academic success has produced mixed outcomes, suggesting that the relationship between levels of naturalness and student success is more complex than previously assumed. Utilizing student surveys collected during 283 EE field trip programs and remotely sensed land cover data, this study examines the relationship between levels of …

They Say, We Say: A Standpoint Analysis Of Stay-At-Home Mothers’ Engagement With Public Discourse, Ayesha Tanzila

All Theses

The purpose of this research was to study stay-at-home mothers’ engagement with the prevailing discourse surrounding them. Staying home and engaging in "invisible and unpaid" labor has led this group of mothers to be out of public sight and somewhat voiceless. Thus, public discourse about SAHMs without significant input by SAHMs has resulted in a monolithic and static identity. Using standpoint analysis as the theoretical framework and textual analysis as the methodology, I have analyzed opinion pieces written by SAHMs, published on popular magazine portals, and on their blogs, through which they attempt to navigate this public depiction of themselves. …

Utility Of Anaerobic Soil Disinfestation For Weed And Disease Management In Solanaceous Vegetable Crops, Gursewak Singh

All Theses

Managing weeds is one of the most challenging aspects of growing specialty crops. Weed control options are limited for specialty crops due to absence of effective herbicide options. The limited current herbicide options risk carryover and damage to subsequent crops. Bacterial wilt is another problem causing significant yield losses in southeast vegetable production. After the termination of methyl bromide due to health and environmental concerns, several other chemical fumigants gained interest, including 1,3-dichloropropene, chloropicrin and metam sodium/potassium. While less toxic to the environment than methyl bromide, these fumigants pose carcinogenic and mutagenic threats. Alternative weed control and diseases management tactics …

The Effect Of Using Palm Oil As An Electron Donor For The Reductive Dechlorination Of Trichloroethylene (Tce), Shivani Swamy

All Theses

TCE is currently remediated using physical methods like air stripping and granular activated carbon adsorption. While physical and chemical processes work on a site-specific basis, biological strategies have been favored as they are economical and reliable. The objective of this work was to determine the feasibility of using palm oil as an electron donor in the reductive dechlorination of TCE to ethene. Palm oil was considered for the study because it is locally grown in many parts of the world. The palm oil that does not meet the industry standards is discarded. Despite the environmental impacts caused by the palm …

Fine Mapping Of Chilling Requirement And Bloom Date To Enable Marker-Assisted Breeding Of Climate Resilient Peach, Gizem Demirel

All Theses

Peach (Prunus persica (L.) Batsch) fruit is a delicious summer treat appreciated for its sweet, juicy, and aromatic taste all over the globe. With only 44 calories and zero fat per fruit, peach has a great popularity among consumers and producers for its nutritional and economic value. Peach production is threatened by unpredictable climate changes, rising temperatures that reduce winter chill, especially in the warmer growing areas, resulting in fruit and yield loss. Therefore, it is necessary to develop cultivars that are resilient to environmental changes. Peach breeding takes a long time, and this process can benefit from the …

Stress And Anxiety Experience Of Dental Hygiene Students, Kristen Ford

Electronic Theses and Dissertations

The ETSU dental hygiene program is very competitive and rigorous for those admitted. Student dental hygienists may experience higher levels of anxiety and more stressors while completing their course work and clinical hours than they have previously experienced in general education and prerequisite courses. These increased number of stressors and higher levels of anxiety may have negative impacts on students’ academic performance and success in clinical learning environments. A better understanding of the actual need for a stress reduction and anxiety management curriculum will facilitate creating and implementing this type of curriculum. A total of 44 current ETSU dental hygiene …

A Causal Comparative Study Of Stem Persistence Between Supported And Non-Supported Stem Interested Students, Elizabeth Bernardi

Electronic Theses and Dissertations

Many students who enter a STEM track in college move out of that track before graduation (National Science Foundation, 2018). The purpose of this study was to assess whether there was a difference in STEM-related major persistence for population proportions of students actively involved in the Science Scholars program and those who were STEM-interested but not program participants. This program oriented students to the STEM program, facilitated engagement with peers and faculty, exposed students to research opportunities, and filled in potential learning gaps (Gibson et al., 2019).

The questions guiding the current research included:

Q1. Was there a statistically significant …

Preparation And Study Of Bacterial Membrane Models, Enoch Asimbisa

Electronic Theses and Dissertations

Fuel molecules are organic solvents that have disruptive effects on the bacterial membrane. This is a significant barrier in biofuel production, as it limits the fuel concentration that can be achieved through fermentation. One potential way of overcoming this barrier is to identify lipid compositions that can better withstand solvent stress, for which it is important to understand how organic solvents disrupt the membrane. Use of biophysical characterization techniques to quantify physical properties like fluidity and thickness will enable us to understand the mechanism by which solvents disrupt membranes. Native membranes are very complex, and we sought to develop in-vitro …

Synthesis Of Phosphatidylethanolamine Lipids For Model Studies Of The Cell Membrane, John Hayford G. Teye-Kau

Electronic Theses and Dissertations

Concerns about global warming have resulted in a surge of research into alternatives to fossil fuels. In recent years, biofuels have gained traction due to their low environmental impact. Biofuel production most commonly employs microorganisms to convert biomass to fuel for industrial and transportation applications. Compounds made in biofuel production, however, are toxic to cell membranes and disrupt their integrity, harming the microorganisms and limiting biofuel yield. A key to overcoming this challenge is understanding how fuels interact with microorganisms’ cell membranes, which perform a host of functions, including transport, cell recognition, transduction, and movement. Phospholipids are the cell membrane’s …

Student Collaboration: Early Childhood Teachers' Roles And Perspectives, Kimberly Ballantyne

Electronic Theses and Dissertations

Early childhood environments can offer valuable opportunities for student collaboration. Social interactions allow students to practice listening to each other and learn how to work together. This study focused on the roles and perspectives of early childhood educators related to student collaboration in the classroom. Six educators from one elementary school in New Hampshire participated in two focus group discussions, the first of which included a presentation on student collaboration with first graders. Participants also completed four concept maps highlighting their perspectives about student collaboration and one written reflection comparing their perspectives before and after engaging in the focus group …

Synthesis Of Phytosulfokine Analogs As Probes For Studying Plant Signaling And Molecular Trafficking, Thomas Ntim

Electronic Theses and Dissertations

Plants are exposed to a wide range of biotic and abiotic stresses that hinder their growth and reduce crop productivity. In their adaptive response, plants use signaling molecules that are trafficked throughout the plant. This research focuses on the chemical synthesis and assessment of analogs of the plant signal phytosulfokine (PSK, a sulfated pentapeptide), its delivery to plants and its observation using a fiber-optic fluorescence microscope. PSK regulates growth, cell expansion, heat tolerance, and tissue longevity. Analogs of PSK were synthesized using solid-phase peptide synthesis. Pure PSK and TAMRA-labeled PSK were delivered into the wild-type Arabidopsis thaliana Col-0 and a …

Facile Nitrogen-Doping Of Screen-Printed Carbon Electrodes For Detection Of Hydrogen Peroxide, Emmanuel Nkyaagye

Electronic Theses and Dissertations

Screen-printed carbon electrodes (SPCEs) have garnered much attention as sensors due to their simplicity and relatively low cost. However, to impart necessary selectivity and sensitivity for specific applications, modification of the SPCE surface, which can involve time-consuming procedures or costly equipment/materials, is typically required. Here, a simple nitrogen-doping process based on NH₄OH was used to modify SPCEs prepared from commercially available ink for electrochemical detection of H₂O₂, a common target for biosensing strategies and indicator of cell stress. XPS studies showed that NH₄OH treatment of SPCEs led to a nearly 5-fold increase …

The Influence Of Social Media On The Tourism Industry: A Content Analysis Of Culinary Tourism Brands Via Instagram, Angela White

Electronic Theses and Dissertations

The tourism industry in the United States is constantly changing and being influenced by social media, specifically Instagram. Culinary brands use Instagram to advertise their restaurants and dishes in a creative, visual way. As a result, Instagram is now being used as a tool by tourists and both potential and current customers to share their dining experiences and find these locations based on the visual appeal. This study provides a content analysis of Instagram account information and photo composition within eight popular culinary tourism destinations by examining the strategies used by the accounts. 50 randomly selected photos were used from …

Reintegrating Biology Through The Nexus Of Energy, Information, And Matter, Kim L. Hoke, Sara L. Zimmer, Adam B. Roddy, Mary Jo Ondrechen, Craig E. Williamson, Nicole R. Buan

Department of Biochemistry: Faculty Publications

Information, energy, and matter are fundamental properties of all levels of biological organization, and life emerges from the continuous flux of matter, energy, and information. This perspective piece defines and explains each of the three pillars of this nexus. We propose that a quantitative characterization of the complex interconversions between matter, energy, and information that compose this nexus will help us derive biological insights that connect phenomena across different levels of biological organization. We articulate examples from multiple biological scales that highlight how this nexus approach leads to a more complete understanding of the biological system. Metrics of energy, information, …