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Shell Calcification And Growth Of The Aquatic Snail Planorbella Trivolvis Under Low Calcium Conditions Typical Of Decalcified Northern Lakes, Shawn A. Witte

Biology Theses

The water calcium concentration of northern softwater lakes has declined to critically low levels due to numerous anthropogenic stressors, and the availability of calcium is expected to decline further. To assess the impact that critically low water calcium has on aquatic snail shell calcification and growth, juvenile Planorbella trivolvis were raised for 60 days in one of four treatments, each containing a different amount of dissolved calcium (0.64, 1.3, 2.5, and 5.1 mg/L). There was no difference in tissue weight or shell size among treatments, suggesting low water calcium concentrations do not impact adult body mass or size. However, snails …

Developmental And Behavioral Responses Of Early Life Stages Of Fathead Minnows (Pimephales Promelas) To Urban Effluents Treated With Advanced-Oxidation Processes, Amy Cavanaugh

Biology Theses

Wastewater Treatment Plants (WWTP) are the main vector for pharmaceutical and personal care products (PPCPs) to enter waterways. Many PPCPs are lipophilic, allowing them to bioaccumulate and biomagnify within aquatic organisms. PPCPs have been known to alter fish behavior and physiological processes, such as nesting defense and sex ratios, and produce an increase in deformities. Advanced oxidation processes (AOPs) are novel ways for treating WWTPs effluents that use hydroxyl radicals to indiscriminately breakdown PPCPs. In this study, fathead minnows (Pimephales promelas) were used to examine the biological effects of AOP-treated municipal effluent as they developed from eggs to …

Formation Of B Horizons In Engineered Putting Green Soils, Glen R. Obear

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Engineered turfgrass putting green soils are designed to drain quickly, while maintaining adequate water- and nutrient-holding capacity to sustain plant growth. These soils are designed to meet specific performance characteristics when they are constructed, but the process of soil formation changes these characteristics over time. Chapter 1 of this dissertation is a literature review of soil formation in engineered putting green soils. Pedogenesis of putting greens is such that A horizons form as organic matter accumulates near the surface, and B horizons form as particles and solutes are translocated to textural or pH boundaries in soil profiles. In the engineered …

American Burying Beetle, Plant Richness, And Soil Property Responses To Collapse Of Juniperus Virginiana Woodlands With Fire, Alison K. Ludwig

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Grasslands are declining in the Great Plains due to land use changes, woody plant encroachment, and loss of historic fire cycles. Prescribed burn associations have utilized prescribed fire to collapse invading woodlands and allow the restoration of grasslands. This fire is considered “extreme” because it is capable of changing the structure and function of an ecosystem. Our study site is the Loess Canyons Experimental Landscape, a long-term, ecoregion-scale experiment to apply prescribed fire across the region to restore grasslands. The Nebraska Natural Legacy Project established the Loess Canyons ecoregion as a Biologically-Unique Landscape in 2005 with the state’s wildlife action …

Livestock Grazing Impacts On Crop And Soil Responses For Two Cropping Systems, Alyssa Kuhn

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Diversified crop, forage, and livestock systems are assumed to be more sustainable and economically competitive than traditional cropping systems. Objectives of this study were to determine effects of integrating grazing livestock into corn (Zea mays)-soybean (Glycine max (L.) Merr.) (C-S) and corn-soybean-wheat (Triticum aestivum L.) (C-S-W) cropping systems on plant population, grain yield, soil nutrients and soil carbon dioxide (CO₂) flux following winter grazing corn residue (both systems) and an oat (Avena sativa) cover crop (C-S-W only) planted after wheat. For the 2019 and 2020 production seasons, neither corn nor soybean plant …

The Indirect Effect Of Childhood Trauma On Body Mass Index Through Exercise Frequency, Andreana Durham

Honors Theses

Objective: Research pertaining to Body Mass Index (BMI) and Childhood Trauma (CT) is very limited, with the main focus being on how these two variables correlate, disregarding other possible covariates. The purpose of the current study was to examine the effects of CT on BMI while including Exercise Frequency as a mediator of this relationship and sex as a moderator for both the direct and indirect relationships among these variables. Methods: Participants included 1,812 individuals (57.9% female and 42.1% male) who completed the Behavior Health Questionnaire, which included questions looking at exercise frequency, CT, and BMI. Results: CT was related …

Occurrence, Diversity And Distribution Of Trypanosoma Infections In Cattle Around The Akagera National Park, Rwanda, S. Richard Gashururu, Ndichu Maingi, Samuel M. Githigia, Methode N. Gasana, Peter O. Odhiambo, Dennis O. Getange, Richard Habimana, Giuliano Cecchi, Weining Zhao, James Gashumba, Joel L. Bargul, Daniel K. Masiga

All Peer-Reviewed Publications

Background African Trypanosomiases threaten the life of both humans and animals. Trypanosomes are transmitted by tsetse and other biting flies. In Rwanda, the African Animal Trypanosomiasis (AAT) endemic area is mainly around the tsetse-infested Akagera National Park (NP). The study aimed to identify Trypanosoma species circulating in cattle, their genetic diversity and distribution around the Akagera NP. Methodology A cross-sectional study was carried out in four districts, where 1,037 cattle blood samples were collected. The presence of trypanosomes was determined by microscopy, immunolog-ical rapid test VerY Diag and PCR coupled with High-Resolution Melt (HRM) analysis. A parametric test (ANOVA) was …

Distribution And Mating Type Characterization Of Chickpea Blight (Didymella Rabiei (Kov.) V. Arx) In Ethiopia, Gezahegne Getaneh, Tadele Tefera, Fikre Lemessa, Seid Ahmed, Jandouwe Villinger

All Peer-Reviewed Publications

We have assessed the distribution and mating type of chickpea blight (Didymella rabiei (Kov.) v. Arx) from a total of 350 and 272 fields in major chickpea (Cicer arietinum L.) growing areas of Ethiopia for two consecutive cropping seasons 2017 and 2018, respectively. The prevalence of D. rabiei was 20 % for the year 2017 and 7% for 2018. Severity range of 2−9 and incidence of 10–100 % were recorded for both seasons in only 7–20 % of the assed fields. Field prevalence of 2017 and 2018 seasons were significantly different (df = 31; p < 0.001 and df = 31; p < 0.001, respectively). Similarly, the disease prevalence of both seasons was significantly different (df = 31; p < 0.001). The disease prevalence ratio frequency was highly deviated from (1:1) for both seasons (X2 = 213.62 p= <0.001; X2 = 144.36 p = 0.01). Genomic DNA of Representative samples were extracted from single spore culture and Mating type 1 (MAT1-1) specific primer SP21, Mating type 2 (MAT1-2) specific primer Tail 5, and a flanking region-specific primer Com1 assay were multiplexed in a single PCR reaction to determine the occurrence of D. rabiei mating type in Ethiopia. Out of 156 samples, only 15 samples were positive to MAT1-1 (∼10 %) with the ratio of 9:1. MAT 1−2 type was the most dominant and possibly the asexual reproduction of D. rabiei is the major type in Ethiopia. The result is important for Ascochyta blight management in breeding strategy.

Gene Co-Expression Network Analysis Of Trypanosoma Brucei In Tsetse Fly Vector, Kennedy W. Mwangi, Rosaline W. Macharia, Joel L. Bargul

All Peer-Reviewed Publications

Background: Trypanosoma brucei species are motile protozoan parasites that are cyclically transmitted by tsetse fly (genus Glossina) causing human sleeping sickness and nagana in livestock in sub-Saharan Africa. African trypanosomes display digenetic life cycle stages in the tsetse fly vector and in their mammalian host. Experimental work on insect-stage trypanosomes is challenging because of the difficulty in setting up successful in vitro cultures. Therefore, there is limited knowledge on the trypanosome biology during its development in the tsetse fly. Consequently, this limits the development of new strategies for blocking parasite transmission in the tsetse fly. Methods: In this study, RNA-Seq …

Transcriptomic Response Of Anopheles Gambiae Sensu Stricto Mosquito Larvae To Curry Tree (Murraya Koenigii) Phytochemicals, Clarence M. Mang’Era, Fathiya M. Khamis, Erick O. Awuoche, Ahmed Hassanali, Fidelis Levi Odhiambo Ombura, Paul O. Mireji

All Peer-Reviewed Publications

Background: Insect growth regulators (IGRs) can control insect vector populations by disrupting growth and development in juvenile stages of the vectors. We previously identified and described the curry tree (Murraya koenigii (L.) Spreng) phytochemical leaf extract composition (neplanocin A, 3-(1-naphthyl)-l-alanine, lumiflavine, terezine C, agelaspongin and murrayazolinol), which disrupted growth and development in Anopheles gambiae sensu stricto mosquito larvae by inducing morphogenetic abnormalities, reducing locomotion and delaying pupation in the mosquito. Here, we attempted to establish the transcriptional process in the larvae that underpins these phenotypes in the mosquito. Methods: We first exposed third-fourth instar larvae of the mosquito to the …

Insect Flower-Visitors Of African Oil Palm Elaeis Guineensis At Different Sites And Distances From Natural Vegetation In Uganda, James Peter Egonyu, Stephen Erisa Sisye, John Baguma, Milton Otema, Gabriel Ddamulira

All Peer-Reviewed Publications

This survey aimed at generating information to guide improvement of oil palm fruit-set in Uganda by testing the hypothesis that diversity and abundance of oil palm insect flower-visitors were influenced by sampling site, distance from natural vegetation and flower sex. Sampling was done on male and female oil palm flowers from (i) five oil palm experimental fields in different districts and (ii) oil palm plantations on Bugala Island on Lake Victoria. The plantations on Bugala Island were subdivided into near (0–15 m) and far (100–200 m) from natural vegetation. We recorded 162,677 insects in 23 morpho-species, dominated by weevils in …

Laurel Wilt In Sassafras Along The Leading Edge Of Disease Progression In The Carolinas, Katy Crout

All Theses

The laurel wilt disease complex consists of a beetle vector (redbay ambrosia beetle (RAB); Xyleborus glabratus Eichhoff (Coleoptera: Curculionidae: Scolytinae)), a symbiotic fungus and tree pathogen (Raffaelea lauricola (Ophiostomataceae) T.C. Harr., Fraedrich & Aghayeva), and host trees (Lauraceae). RAB originated from Asia and was first discovered in the United States near Savannah, Georgia in 2002. RAB deposits R. lauricola in host trees, including redbay (Persea borbonia) and sassafras (Sassafras albidum), when females tunnel into trees to lay eggs. Xyleborus glabratus attacking sassafras may have far reaching ecosystem impacts due to the expansive range of sassafras in the eastern United States …

In The Pines: Dung Beetle Community Composition In Longleaf Pine Habitat, Kelsea Lynn Young

All Theses

Dung beetle assemblages are recognized as ecological bioindicators, allowing us to monitor ecological impacts by observing community shifts. Acting as primary nutrient cyclers, they establish niche segregation by sometimes selecting dung based on species (e.g. coyote vs. deer) and by using dung in different ways (e.g. rollers vs. tunnelers). Longleaf pine savannahs are recognized as global hotspots for diversity. Species of dung beetles found within these ecosystems may differ substantially from those found in surrounding agricultural, residential or other forest land. Our objective was to investigate whether the exclusion of mammalian meso-predator exclusion impacts dung beetle abundance, species composition and …

Effect Of Metal Ions On Stability Of Ascorbic Acid And Niacin Determined By Hplc, Jhongyan Huang

All Theses

Water-soluble vitamins, such as vitamin C and vitamin B, exist in various food stuffs, including vegetables, fruits, fortified grains, milk, and meat, etc. Those vitamers play essential roles in many biochemical functions in human body, thus are significant components for maintaining human health. However, the stability of vitamers is susceptible to many factors, such as light, temperature, metal ions, levels of oxygen concentration, and moisture contents, etc. As vitamers are prone to further degradation, their bioactivities can be remarkably affected and even lost in food processing and food products.In this study, effect of different metal ions (i.e., Na+, K+, Ca2+, …

Water Recovery From Bioreactor Mixed Liquors Using Forward Osmosis With Polyelectrolyte Draw Solutions, Calen Raulerson

All Theses

The objective of this research was to test the feasibility of using forward osmosis (FO) with polyelectrolyte draw solutions to recover water from bioreactor mixed liquors. When combined with an Anaerobic Osmotic Membrane Bioreactor (AnOMBR), such a system could process fecal and food waste from astronauts aboard the International Space Station (ISS) and reclaim important nutrients and water. This project focused on measuring the obtainable water recovery rates from bioreactor effluent, and the identifying challenges associated with the operation.

AnOMBRs feature several advantages over aerobic bioreactors, and non-osmotic anaerobic membrane bioreactors (AnMBR). Anaerobic bioreactors avoid the significant energy costs of …

An Analysis Of Environmental Conditions Impacting Cyanobacterial Algal Blooms In Drinking Water Sources In Upstate South Carolina, Gregory Langlois

All Theses

Maintaining water quality in reservoirs used for drinking water has been an issue in recent years due to the presence of algal blooms. Algal blooms are a perennially recurring problem that can have negative impacts on tourism, recreation, and overall water quality. Additionally, algal blooms will often produce an assortment of chemicals, some of which are hazardous to the health of humans, and some of which that, while relatively innocuous, result in unpleasant tastes and odors in water. Geosmin and 2-methylisoborneol are two taste and odor compounds that are notoriously difficult to treat out of drinking water sources by traditional …

Damned Ol' Dirt, Molly Morningglory

All Theses

damned ol’ dirt centers mindfulness and embodiment practices to foster relationships with the self, each other, and the land. These relationships intend to collectively imagine and then build an emotionally and environmentally sustainable and joyful future. My practice foregrounds clay with digital video, photography, and fabric dyeing, recording the imprint of performance. I use my hereditary understanding of clay and fibers, a trained attunement to the natural world, and my background of performance in craft (via demonstration of tactile techniques) to transfer knowledge and skill to the viewer. Through the creation of tableaus and documents of rituals based in materiality, …

Effects Of Cashew Nut Shell Extract On Nutrient Digestibility And Ruminal Fermentation Under In Vitro Batch Culture And Continuous Culture Conditions, Chandler Compton

All Theses

The overall objective of this research was to determine the effects of cashew nut shell extract (CNSE; 59% anacardic acid and 18% cardol) on nutrient digestibility and rumen fermentation profile under in vitro batch culture (Chapter 2) and continuous culture conditions (Chapter 3). The second objective was to determine an optimal supplementation dosage for dairy cows. The batch culture study was organized as a randomized complete block design with 15 replicates per treatment incubated for 24 h during four incubation runs. Each incubation was inoculated with rumen contents collected from ruminally fistulated cows fed either a close-up ( …

Gender Identity And Mental Health Among Undergraduate Students In The United States, Mckenzie Mcnamara

All Theses

This study explores the relationship between gender identity, challenges experienced by students, psychological distress, and suicide behavior for undergraduate students in the United States of America. The quantitative analysis utilizing the American College Health Association’s National College Health Assessment from Fall 2019, Spring 2020 and Fall 2020. The sample consisted of 78,296 undergraduate participants of which 65.9% identified as female, 30.5% identified as male, and 3.6% identified as non-binary students. The statistical analysis consisted of a multiple regression model controlling for variables of institution type. The major finding of this study is that non-binary undergraduate students had greater psychological distress …

Development And Validation Of An Analytical Modeling Tool For Solar Borehole Heat Exchangers, Adam Ornelles

All Theses

Current numerical modeling solutions used for subsurface heat flow, such as the Berkeley TOUGH codes, are often difficult to use and time-consuming compared to analytical modeling methods. However, current analytical modeling methods for subsurface heat flow and groundwater cannot accurately model the subsurface in 3 dimensions, a feature paramount to the planning and installation of thermal borehole heat exchangers used in environmental remediation.

This research developed a novel 3-dimensional analytical modeling tool for solar-powered borehole heat exchangers. The analytical modeling tool can plan thermal remediation systems while being more straightforward to operate than current numerical modeling software. By creating a …

Studies In Consumption And Production Of South Carolina Aquaculture Products, David Cheplick

All Theses

Market demand for seafood products among South Carolina (SC) consumers has led to increased aquaculture production as a means of supporting the seafood supply chain across the state. Our objective is broken into four distinct parts; (1) understanding consumer’s preferences and perceptions of seafood, (2) calculating their willingness to pay (WTP) for seafood products available in SC using attributes of sustainability and locality; and (3) gathering production metric and anecdotal data from SC oyster growers. Previous research assessing the market channels of seafood in South Carolina is compared to survey results. Respondents indicated that taste and quality were the most …

Investigation On Effects Of Lactate Adaptation Using Metabolic Indicators And Amino Acids To Explore Cell Metabolism, Lisa Uy

All Theses

Biopharmaceutical products are drugs that are made from living cells. One protein type of high importance to biopharmaceuticals is monoclonal antibodies. Monoclonal antibodies allow for highly specific, targeted treatment of diseases and detection of molecules related to diseases. With major roles in detecting and/or treating diseases like human immunodeficiency virus (HIV), coronavirus disease 2019 (COVID-19), and some cancers, monoclonal antibodies are in demand. This demand has led to a need for efficient cell culture systems to produce high yields of protein with minimal waste. The most commonly used cell culture system for these high-value proteins is Chinese hamster ovary (CHO) …

Environmental Law, Disrupted By Covid-19, Rebecca Bratspies, Vanessa Casado-Pérez, Robin Kundis Craig, Lissa Griffin, Keith Hirokawa, Sarah Krakoff, Katrina Kuh, Jessica Owley, Melissa Powers, Shannon Roesler, Jonathan Rosenbloom, J.B. Ruhl, Erin Ryan, David Takacs

Faculty Scholarship

As we were in the final phases of editing a book on disruption in environmental law, a pandemic swept across the world disrupting daily life and the functioning of society to an extent unprecedented in living memory. The novel coronavirus known as COVID-19 was identified in China in late 2019 and by late February 2020, it had spread to every continent except Antarctica; as of April, 2021, the World Health Organization (WHO) estimated that over 148 million people had been infected worldwide with over 3 million deaths. Scientists and public health experts have raced to understand the virus—how is it …

Recent Updates In Management Of Borderline Ovarian Tumors, Sara Abodabesh, Khalid Gaballah, Ashraf Shoma

Mansoura Medical Journal

Borderline ovarian tumors(BOTs) are the epithelial neoplasm which need to be differentiated from ovarian carcinomas as their presentation and management differs from each other. The important differentiating feature on histopathological examination is the absence of stromal invasion. Considering the degree of aggressiveness between epithelial ovarian carcinoma and benign ovarian neoplasms like cystadenoma, borderline neoplasms are closer to benign ovarian neoplasms as regards the clinical behavior. Surgery with intention of removal of all visible tumors is the most important pillar in treatment. Patients treated conservatively with fertility sparing surgery and good results with laparoscopic surgery has low recurrence rate which makes …

Efficacy Of Sediment Contaminant Remediation Of The Benthos In A Segment Of The Southern Branch Of The Elizabeth River, Colton Martin

Biological Sciences Theses & Dissertations

The bottom sediment of the Southern Branch of the Elizabeth River, a tributary of Chesapeake Bay, was historically contaminated with hydrocarbons from industrial sources especially wood treatment facilities. The Elizabeth River Project selected a section of the bottom off Money Point in the Southern Branch for a sediment contaminant remediation effort. Prior to initiation of remediation efforts, a survey occurred in summer 2010 to characterize the ecological condition of the benthic communities off Money Point compared to benthic communities of a benthic region across the channel and northwest of Money Point near Blows Creek. That study characterized the benthos of …

Population Connectivity In Voles (Microtus Sp.) As A Gauge For Tall Grass Prairie Restoration In Midwestern North America, Marlis R. Douglas, Steven M. Mussmann, Tyler Chafin, Whitney J.B. Anthonysamy, Mark A. Davis, Matthew P. Mulligan, Robert L. Schooley, Wade Louis, Michael E. Douglas

Biological Sciences Faculty Publications and Presentations

Ecological restoration can promote biodiversity conservation in anthropogenically fragmented habitats, but effectiveness of these management efforts need to be statistically validated to determine ’success.’ One such approach is to gauge the extent of recolonization as a measure of landscape permeability and, in turn, population connectivity. In this context, we estimated dispersal and population connectivity in prairie vole (Microtus ochrogaster; N = 231) and meadow vole (M. pennsylvanicus; N = 83) within five tall-grass prairie restoration sites embedded within the agricultural matrix of midwestern North America. We predicted that vole dispersal would be constrained by the …

Cnidarian Hair Cell Development Illuminates An Ancient Role For The Class Iv Pou Transcription Factor In Defining Mechanoreceptor Identity, Ethan Ozment, Arianna N. Tamvacakis, Jianhong Zhou, Pablo Yamild Rosiles-Loeza, Estaban Elias Escobar-Hernandez, Selene L. Fernandez-Valverde, Nagayasu Nakanishi

Biological Sciences Faculty Publications and Presentations

Although specialized mechanosensory cells are found across animal phylogeny, early evolutionary histories of mechanoreceptor development remain enigmatic. Cnidaria (e.g. sea anemones and jellyfishes) is the sister group to well-studied Bilateria (e.g. flies and vertebrates), and has two mechanosensory cell types - a lineage-specific sensory effector known as the cnidocyte, and a classical mechanosensory neuron referred to as the hair cell. While developmental genetics of cnidocytes is increasingly understood, genes essential for cnidarian hair cell development are unknown. Here, we show that the class IV POU homeodomain transcription factor (POU-IV) - an indispensable regulator of mechanosensory cell differentiation in Bilateria and …

Comparative Metabolism Of Aflatoxin B1 In Two Quail Genera Coturnix Japonica And Callipepla Californica, Sean Moody

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Avian species are highly susceptible to the hepatotoxic mycotoxin aflatoxin B₁ (AFB₁). Domesticated turkeys are exquisitely sensitive, due to a combination of highly-efficient hepatic cytochrome P450 (CYP)-mediated bioactivation, and to dysfunctional alpha-class glutathione S-transferases (GSTAs) which typically detoxify the bioactivated electrophilic metabolite exo-AFB₁-8,9-epoxide (AFBO). Wild turkeys are relatively resistant to AFB₁ in large part due to expression of functional GSTAs. Quail, a related Galliforme, are slightly less sensitive in vivo to AFB₁, but whether this is related to the hepatic metabolic profiles of these two critical enzymes has not been rigorously evaluated. …

Progress And Prospect Of Anodic Oxidation For The Remediation Of Perfluoroalkyl And Polyfluoroalkyl Substances In Water And Wastewater Using Diamond Electrodes, Sean T. Mcbeath, Adrián Serrano Mora, Fatemeh Asadi Zeidabadi, Brooke K. Mayer, Patrick J. Mcnamara, Madjid Mohseni, Michael R. Hoffman, Nigel J. D. Graham

Civil and Environmental Engineering Faculty Research and Publications

Although diamond electrodes are widely used in the field of electroanalysis and sensing, their application in the field of environmental engineering has yet to be fully realized. Many research studies have considered their potential application in water and wastewater treatment, where the in-situ electrochemical process can avoid the need for chemical additives by facilitating the oxidation of pollutants on the electrode surface or mediated by electrochemically synthesized oxidants in solution. Diamond-based electro-oxidation can effectively treat a number of organic micropollutants and is now being evaluated for the abatement of perfluoroalkyl and polyfluoroalkyl substances, which pose health concerns and are ubiquitous …

Open System Metabolism Of The Grand River From Headwaters To Mouth, Colin J. Assenmacher

Honors Projects

The Grand River is the longest river in Michigan and has been greatly impacted by human activities, particularly logging in the mid-1800s, which when coupled with 20th-century urbanization and continued agricultural use, led to historically poor river health. Despite this, actions throughout the past 50 years by federal, state, and local citizen involvement, have resulted in increased river health and broader water quality monitoring within the watershed. During the summer of 2021, rapid bioassessments targeting benthic macroinvertebrates were conducted along the Grand River, with the primary goal of following up on prior state-led surveys conducted at different locations along the …