Digital Commons Network^™

Donor Chimerism And Immune Reconstitution Following Haploidentical Transplantation In Sickle Cell Disease, Yaya Chu, Brenda Grossman, Shalini Shenoy, Et Al

2020-Current year OA Pubs

INTRODUCTION: We previously reported the initial results of a phase II multicenter transplant trial using haploidentical parental donors for children and aolescents with high-risk sickle cell disease achieving excellent survival with exceptionally low rates of graft-versus-host disease and resolution of sickle cell disease symptoms. To investigate human leukocyte antigen (HLA) sensitization, graft characteristics, donor chimerism, and immune reconstitution in these recipients.

METHODS: CD34 cells were enriched using the CliniMACS

RESULTS: HLA antibodies were detected in 7 of 11 (64%) evaluable patients but rarely were against donor antigens. Myeloid engraftment was rapid (100%) at a median of 9 days. At 30 …

Postnatal Osterix But Not Dmp1 Lineage Cells Significantly Contribute To Intramembranous Ossification In Three Preclinical Models Of Bone Injury, Evan G Buettmann, Susumu Yoneda, Pei Hu, Jennifer A Mckenzie, Matthew J Silva

2020-Current year OA Pubs

Murine models of long-bone fracture, stress fracture, and cortical defect are used to discern the cellular and molecular mediators of intramembranous and endochondral bone healing. Previous work has shown that Osterix (Osx

Top-Down And Bottom-Up Propagation Of Disease In The Neuronal Ceroid Lipofuscinoses, John R Ostergaard, Hemanth R Nelvagal, Jonathan D Cooper

2020-Current year OA Pubs

BACKGROUND: The Neuronal Ceroid Lipofuscinoses (NCLs) may be considered distinct neurodegenerative disorders with separate underlying molecular causes resulting from monogenetic mutations. An alternative hypothesis is to consider the NCLs as related diseases that share lipofuscin pathobiology as the common core feature, but otherwise distinguished by different a) initial anatomic location, and b) disease propagation.

METHODS: We have tested this hypothesis by comparing known differences in symptomatology and pathology of the CLN1 phenotype caused by complete loss of

RESULTS: Despite displaying similar pathological endpoints, the clinical phenotypes and the evidence of imaging and

DISCUSSION: The propagation of disease in these two …

Stat3 Gain-Of-Function Syndrome, Tiphanie P Vogel, Jennifer W Leiding, Megan A Cooper, Lisa R Forbes Satter

2020-Current year OA Pubs

STAT3 gain-of-function (GOF) syndrome is a multi-organ primary immune regulatory disorder characterized by early onset autoimmunity. Patients present early in life, most commonly with lymphoproliferation, autoimmune cytopenias, and growth delay. However, disease is often progressive and can encompass a wide range of clinical manifestations such as: enteropathy, skin disease, pulmonary disease, endocrinopathy, arthritis, autoimmune hepatitis, and rarely neurologic disease, vasculopathy, and malignancy. Treatment of the autoimmune and immune dysregulatory features of STAT3-GOF patients relies heavily on immunosuppression and is often challenging and fraught with complications including severe infections. Defects in the T cell compartment leading to effector T cell accumulation …

Low Risk For Developing Diabetes Among The Offspring Of Individuals With Exceptional Longevity And Their Spouses, Iva Miljkovic, Ryan Cvejkus, Ping An, Bharat Thyagarajan, Kaare Christensen, Mary Wojczynski, Nicole Schupf, Joseph M Zmuda

2020-Current year OA Pubs

Little is known about the risk of type 2 diabetes (T2D) among the offspring of individuals with exceptional longevity. We determined the incidence of and potential risk and protective factors for T2D among the offspring of probands and offspring's spouses (mean age=60 years, range 32-88 years) in the Long Life Family Study (LLFS), a multicenter cohort study of 583 two-generation families with a clustering of healthy aging and exceptional longevity. Incident T2D was defined as fasting serum glucose ≥126 mg/dl, or HbA1c of ≥6.5%, or self-reported with doctor diagnosis of T2D, or the use of anti-diabetic medication during a mean …

Increased Virus Dissemination Leads To Enhanced Lung Injury But Not Inflammation During Influenza-Associated Secondary Bacterial Infection, Amanda P Smith, Lindey C Lane, Ivan Ramirez Zuniga, David M Moquin, Peter Vogel, Amber M Smith

2020-Current year OA Pubs

Secondary bacterial infections increase influenza-related morbidity and mortality, particularly if acquired after 5-7 d from the viral onset. Synergistic host responses and direct pathogen-pathogen interactions are thought to lead to a state of hyperinflammation, but the kinetics of the lung pathology have not yet been detailed, and identifying the contribution of different mechanisms to disease is difficult because these may change over time. To address this gap, we examined host-pathogen and lung pathology dynamics following a secondary bacterial infection initiated at different time points after influenza within a murine model. We then used a mathematical approach to quantify the increased …

Fibroblast Growth Factor Receptor Signaling In Cardiomyocytes Is Protective In The Acute Phase Following Ischemia-Reperfusion Injury, Dzmitry Matsiukevich, Stacey L House, Carla Weinheimer, Attila Kovacs, David M Ornitz

2020-Current year OA Pubs

Fibroblast growth factor receptors (FGFRs) are expressed in multiple cell types in the adult heart. Previous studies have shown a cardioprotective effect of some FGF ligands in cardiac ischemia-reperfusion (I/R) injury and a protective role for endothelial FGFRs in post-ischemic vascular remodeling. To determine the direct role FGFR signaling in cardiomyocytes in acute cardiac I/R injury, we inactivated

Weakly Activated Core Neuroinflammation Pathways Were Identified As A Central Signaling Mechanism Contributing To The Chronic Neurodegeneration In Alzheimer's Disease, Fuhai Li, Abdallah M Eteleeb, William Buchser, Christopher Sohn, Guoqiao Wang, Chengjie Xiong, Philip R Payne, Eric Mcdade, Celeste M Karch, Oscar Harari, Carlos Cruchaga

2020-Current year OA Pubs

OBJECTIVES: Neuroinflammation signaling has been identified as an important hallmark of Alzheimer's disease (AD) in addition to amyloid β plaques (Aβ) and neurofibrillary tangles (NFTs). However, the molecular mechanisms and biological processes of neuroinflammation remain unclear and have not well delineated using transcriptomics data available. Our objectives are to uncover the core neuroinflammation signaling pathways in AD using integrative network analysis on the transcriptomics data.

MATERIALS AND METHODS: From a novel perspective, i.e., investigating weakly activated molecular signals (rather than the strongly activated molecular signals), we developed integrative and systems biology network analysis to uncover potential core neuroinflammation signaling targets …

Actin Capping Protein Regulates Postsynaptic Spine Development Through Cpi-Motif Interactions, Kenneth R Myers, Yanjie Fan, Patrick Mcconnell, John A Cooper, James Q Zheng

2020-Current year OA Pubs

Dendritic spines are small actin-rich protrusions essential for the formation of functional circuits in the mammalian brain. During development, spines begin as dynamic filopodia-like protrusions that are then replaced by relatively stable spines containing an expanded head. Remodeling of the actin cytoskeleton plays a key role in the formation and modification of spine morphology, however many of the underlying regulatory mechanisms remain unclear. Capping protein (CP) is a major actin regulating protein that caps the barbed ends of actin filaments, and promotes the formation of dense branched actin networks. Knockdown of CP impairs the formation of mature spines, leading to …

One Vaccine To Counter Many Diseases? Modeling The Economics Of Oral Polio Vaccine Against Child Mortality And Covid-19, Angela Y Chang, Peter Aaby, Michael S Avidan, Christine S Benn, Stefano M Bertozzi, Lawrence Blatt, Konstantin Chumakov, Shabaana A Khader, Shyam Kottilil, Madhav Nekkar, Mihai G Netea, Annie Sparrow, Dean T Jamison

2020-Current year OA Pubs

INTRODUCTION: Recent reviews summarize evidence that some vaccines have heterologous or non-specific effects (NSE), potentially offering protection against multiple pathogens. Numerous economic evaluations examine vaccines' pathogen-specific effects, but less than a handful focus on NSE. This paper addresses that gap by reporting economic evaluations of the NSE of oral polio vaccine (OPV) against under-five mortality and COVID-19.

MATERIALS AND METHODS: We studied two settings: (1) reducing child mortality in a high-mortality setting (Guinea-Bissau) and (2) preventing COVID-19 in India. In the former, the intervention involves three annual campaigns in which children receive OPV incremental to routine immunization. In the latter, …

The Enantiomer Of Allopregnanolone Prevents Pressure-Mediated Retinal Degeneration Via Autophagy, Makoto Ishikawa, Toru Nakazawa, Hiroshi Kunikata, Kota Sato, Takeshi Yoshitomi, Kathiresan Krishnan, Douglas F Covey, Charles F Zorumski, Yukitoshi Izumi

2020-Current year OA Pubs

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Semi-Quantitative Assay To Measure Urease Activity By Urinary Catheter-Associated Uropathogens, Jesus M Duran Ramirez, Jana Gomez, Chloe L P Obernuefemann, Nathaniel C Gualberto, Jennifer N Walker

2020-Current year OA Pubs

Catheter-associated urinary tract infections (CAUTIs) are one of the most common healthcare-associated infections in the US, accounting for over 1 million cases annually and totaling 450 million USD. CAUTIs have high morbidity and mortality rates and can be caused by a wide range of pathogens, making empiric treatment difficult. Furthermore, when urease-producing uropathogens cause symptomatic CAUTI or asymptomatic catheter colonization, the risk of catheter failure due to blockage increases. The enzyme urease promotes catheter blockage by hydrolyzing urea in urine into ammonia and carbon dioxide, which results in the formation of crystals that coat the catheter surface. If CAUTI is …

Epithelial Abnormalities In The Small Intestine Of Zambian Children With Stunting, Chola Mulenga, Sanja Sviben, Kanta Chandwe, Beatrice Amadi, Violet Kayamba, James A J Fitzpatrick, Victor Mudenda, Paul Kelly

2020-Current year OA Pubs

BACKGROUND: Environmental enteropathy (EE) contributes to impaired linear growth (stunting), in millions of children worldwide. We have previously reported that confocal laser endomicroscopy (CLE) shows fluorescein leaking from blood to gut lumen

METHODS: We performed confocal laser endomicroscopy (CLE) in 75 children and collected intestinal biopsies for histology in 91 children. CLE videos were evaluated, employing the Watson score to determine severity of leakiness. Morphometry was carried out on well-orientated mucosa and 3 biopsies were examined by electron microscopy.

RESULTS: Confocal laser endomicroscopy demonstrated substantial leakage from circulation to gut lumen in 73 (97%) children. Histology consistently showed characteristic changes …

Loss Of Function Of Wfs1 Causes Er Stress-Mediated Inflammation In Pancreatic Β-Cells, Shuntaro Morikawa, Lindsey Blacher, Chinyere Onwumere, Fumihiko Urano

2020-Current year OA Pubs

Wolfram syndrome is a rare genetic disorder characterized by juvenile-onset diabetes mellitus, optic nerve atrophy, hearing loss, diabetes insipidus, and progressive neurodegeneration. Pathogenic variants in the

Functional And Phylogenetic Characterization Of Bacteria In Bovine Rumen Using Fractionation Of Ruminal Fluid, Ruth Hernández, Maryam Chaib De Mares, Hugo Jimenez, Alejandro Reyes, Alejandro Caro-Quintero

2020-Current year OA Pubs

Cattle productivity depends on our ability to fully understand and manipulate the fermentation process of plant material that occurs in the bovine rumen, which ultimately leads to the improvement of animal health and increased productivity with a reduction in environmental impact. An essential step in this direction is the phylogenetic and functional characterization of the microbial species composing the ruminal microbiota. To address this challenge, we separated a ruminal fluid sample by size and density using a sucrose density gradient. We used the full sample and the smallest fraction (5%), allowing the enrichment of bacteria, to assemble metagenome-assembled genomes (MAGs). …

Introducing Undergraduates To Instructional Design In A Graduate Studio: An Experiential, Model-Centered Approach, Rebecca Stull Zundell, William Sowards, Scott L. Howell, Jason K. Mcdonald

Faculty Publications

This case study describes a combined graduate and undergraduate instructional design studio that introduced undergraduate students to instructional design in a multifaceted, holistic, and applied way. Reviewing the experience of the undergraduates in the course, this design case describes four learning interventions used to create this applied experience: (1) instructional design team projects—one non-profit and the other in higher education, (2) weekly seminars and biweekly training sessions from field experts, (3) an experiential out-of-state trip, and (4) weekly reflection journals. These studio-based learning interventions are presented within the context of the Experiential Learning Theory and Model-Centered Instruction. Overall, the course …

Ecology Of Family Experiences: Contextualizing Family Leisure For Human Development & Family Relations, Karen K. Melton, Camilla Hodge, Mat D. Duerden

Faculty Publications

All family leisure is not equal. Yet, little is known about the mechanisms linking different types of family leisure to different human development and family relations outcomes. The Ecology of Family Experiences (EFE) framework provides a sensitizing lens for the study of the conditions under which family leisure is beneficial to individuals and families. Specifically, the framework suggests three domains—Family, Activity, and Time—iteratively interact to generate different levels of individual and family outcomes (i.e., responses, meaning, and development). The EFE necessitates research built on ecologically valid study designs that incorporate innovative measurement approaches. Scholars using such approaches can increase the …

Redirected Utopias: The Politics Of Self-Reflexive Autobiographical Documentary In Albertina Carri’S Los Rubios And Ufuk Emiroglu’S Mon Père, La Révolution, Et Moi, Stephanie Pridgeon

All Faculty Scholarship

Twenty-first century documentary production from around the world has used self-reflexivity to challenge assumptions about subjectivity and social positioning in ways that explicitly challenge the politics of filmmaking. Released in 2003 and 2013 respectively, Argentine director Albertina Carri’s Los rubios and Turkish filmmaker Ufuk Emiroglu’s Mon père, la révolution et moi incorporate fictions and fantasies into their autobiographical stories, telling their life stories through alternative narrative forms that deviate from existing social and aesthetic precepts. The two filmmakers seek to understand–and scrutinize–the utopian ideals of the revolutionary movements to which their fathers belonged in 1970s Argentina and Turkey, respectively. The …

Vaping Prevalence Among College Students And Their Knowledge Of The Associated Risk Of Vaping, Alicia P. Harris, Sarah V. Allman, Laura L. Guidry, Leah H. Nabors

MSN Research Projects

E-cigarettes are electronic devices that heat a liquid and produce a vapor that can be inhaled. Vaping is a term used in association with use of e-cigarettes. Vaping has become widespread among the young adult population. In 2018, the Food and Drug Administration and Surgeon General declared vaping an epidemic. The Centers for Disease Control and Prevention predicts at the current rate of use among United States adolescents, 5.6 million under the age of 18 will face early death due to vaping. The purpose of this study was to determine vaping prevalence and vaping knowledge among college students. The study …

Updates On Hydroxychloroquine In Prevention And Treatment Of Covid-19, Heather Johnson

All Publications

No abstract provided.

Juntos Luchamos: A Postcritical Ethnographic And Photovoice Study On Latinx Student Civic Engagement Practices At A Hispanic-Serving Institution, Liliana Diaz

Electronic Theses and Dissertations

Situated at a Hispanic-Serving Institution (HSI), this postcritical ethnographic and photovoice study sought to explore how Latinx students define and practice civic engagement. Theoretically framed by Latino Cultural Citizenship (LCC), the study explored how current Latinx student civic engagement practices inform a Hispanic-Serving Institutions’ civic engagement efforts. Data collection took place over the 2021-2022 academic year and an exhibition of the study’s findings was made publicly available at the culmination of the study. Findings from the study indicate that Latinx postsecondary students define civic engagement as knowledge and resource sharing (KRS) and achieving success. Findings for how Latinx postsecondary students …

Differentiating The Effects Of Two Non-Native Fish In The Turnbull National Wildlife Refuge, Sarah Richardson

EWU Masters Thesis Collection

This study seeks to determine the individual effects of two non-native fish, the brook stickleback, Culaea inconstans, and pumpkinseed, Lepomis gibbosus, on the aquatic invertebrates and food webs of Turnbull National Wildlife Refuge (TNWR). Ten ponds were analyzed and compared with stable isotope analysis and invertebrate abundance data. Three ponds were chosen to represent each of the following categories: fishless, brook stickleback invaded (BS), and coinvaded, along with a single pumpkinseed (PS) pond. Overall, δ[superscript]13C and δ[superscript]15N values increased in invaded ponds, indicating shifts in the zooplankton community structure and the carbon source of predatory invertebrates. Mean δ[superscript]13C was the …

Behavioral Differences In Urban Sciurus Carolinensis With Varying Human Exposure As A Model For Synanthropic Human-Animal Relationships, Tiffany M. Jordan

EWU Masters Thesis Collection

Synanthropic animals are considered a halfway point between wild and domestic animals that live in urban environments and depend on humans for their survival. Sciurus carolinensis, the eastern gray squirrel, symbolizes a distinctive coexistence between human and wildlife as they are commonly fed in parks and gardens. They are native to the eastern United States. Where they are invasive, they are found only in urban areas and not in rural or wildlife areas. This study examined how different levels of human exposure impact the behavior of S. carolinensis in its non-native range from two different perspectives by using flight initiation …

Plant-Pollinator Interaction Networks In Remnant And Reconstructed Palouse Prairie In Eastern Wa, Usa, Emma Hoskins

EWU Masters Thesis Collection

Ecological restoration is often focused on re-establishing native plant communities, without considering the larger ecological community, specifically pollinators, despite their being critical to native forb reproduction. Further, ecological restoration has the potential to mitigate global pollinator declines by rebuilding native ecosystems, providing pollinators with floral resources and nesting habitat. This is especially true for prairie restoration, which struggles to establish diverse, abundant native forb communities. The USDA Conservation Reserve Program State Acres for Wildlife Enhancement (CRP SAFE) Palouse Prairie Project provides funding to convert agricultural fields into native Palouse Prairie habitat for the benefit of wildlife. Due to financial and …

Assessing The Behavioral Response Of Westslope Cutthroat Trout To Olfactory Cues In A Two-Choice Y-Maze, Hannah Marie Condron

EWU Masters Thesis Collection

The goal of this study was to determine which olfactory cues were most attractive to Westslope Cutthroat Trout Oncorhynchus clarkii lewisi (WCT) in a two-choice Y-maze. WCT used in this study were from Kings Lake and Paqua Creek in Pend Oreille County, Washington, and tested in 2018 and 2019. The different odorants tested in this study were population-specific pheromones produced by WCT from the same population, conspecific pheromones produced by WCT from the second population, and water obtained from each population’s natal stream. The water Kings Lake WCT were reared in for one year at the Spokane Hatchery was also …

Population Assignment Of Entrained Migratory Westslope Cutthroat Trout In North Idaho, Craig D. Wells

EWU Masters Thesis Collection

The Clark Fork-Pend Oreille River basin of northeastern Washington and the Idaho Panhandle historically supported a robust metapopulation of Westslope Cutthroat Trout (WCT) Oncorhynchus clarkii lewisi, a western native salmonid of high cultural and economic value. The construction of impassible hydroelectric dams and smaller in-stream barriers has prevented the return of migratory WCT to spawning tributaries, likely leading to the fragmentation of this metapopulation over the last 100 years. One such impassible barrier is Albeni Falls Dam near Newport, Washington. Completed without fish passage capabilities in 1955, Albeni Falls Dam has been of interest as a site for restoring fish …

Modernizing Fish Surveys: Evaluating How Reliable Environmental Dna Sampling Is In Monitoring Fish Populations, Alicia Cozza

EWU Masters Thesis Collection

No abstract provided.

Investigating How Bat Ectoparasites Influence The Skin Microbiome Diversity And Composition In Washington State Bats, Dana E. Colley

EWU Masters Thesis Collection

White-Nose Syndrome (WNS), caused by the psychrophilic pathogenic fungus Pseudogymnoascus destructans (Pd), has killed millions of bats in the eastern United States since its initial introduction in 2006 and recent expansion into the western U.S. Understanding factors that contribute to the spread of Pd and risk of infection is crucial for management of WNS. Bat ectoparasites, including bat mites and bat flies, are omnipresent in bat populations, yet the relationship between these ectoparasites and bat health is still unknown. We examined the relationship between bat ectoparasites and the skin microbiome in relation to WNS infection risk in Washington State bats. …

Role Of Seed Mixes, Biochar Soil Amendment, And The Soil Seed Bank In Improving Palouse Prairie Restoration, Kristine Snyder

EWU Masters Thesis Collection

The Palouse Prairie Ecoregion of Eastern Washington and Idaho is critically endangered. Eastern Washington University (EWU) has begun a ~120 acre campus prairie restoration; however, there is considerable uncertainty about the most effective restoration approach. Annual seeds and soil organic matter are important for succession and soil moisture, but their roles are unknown for Palouse prairie restoration. Additionally, the soil seedbank of the Palouse ecoregion is widely unknown but could provide insight into methods or species to use for restoration. My research aimed to determine the effects of seed mix diversity, annual seed addition, and soil organic matter (as biochar) …

Effects Of Gaba On Inflammation And Intestinal Barrier Disruption, Tyrel W. Long

EWU Masters Thesis Collection

Gut dysbiosis and intestinal barrier disruption have been linked to multiple sclerosis (MS). Our previous works show that experimental autoimmune encephalomyelitis (EAE) induction modifies the gut's microbiota composition, reducing frequencies of gamma-aminobutyric acid (GABA)-producing bacteria. GABA levels are reduced in the brains and circulation of MS patients. We engineered Lactococcus lactis with extra copies of gadB (glutamic acid decarboxylase) and gadC (glutamate/GABA antiporter) to increase GABA levels produced by the bacterium (GAD-L. lactis). EAE studies showed that the treatment with GAD-L. lactis and not with a L. lactis control expressing an empty plasmid (P-L. lactis) reduced the severity of the …