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D-Lactic Acid Fermentation Platform From Oil Palm Trunk Sap By Sporolactobacillus Terrae Sbt-1, Naphatsawan Sukaiem Jan 2022

D-Lactic Acid Fermentation Platform From Oil Palm Trunk Sap By Sporolactobacillus Terrae Sbt-1, Naphatsawan Sukaiem

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lactic acid is an organic acid that contains two enantiomers, L- and D-lactic acid, and is considered the most potential monomer for many industrial products. Lactic acid has versatile applications in the pharmaceutical, food, textile, chemical, and leather industries moreover in poly-lactic acid (PLA) manufacturing. While L-lactic acid is well known in many commercial products and studies, only a few studies on D-lactic acid were found. Lactic acid can be produced either by microbial fermentation or chemical synthesis. Approximately 90 percent of the total lactic acid produced worldwide is via microbial fermentation which provides an optically pure L- or D-lactic …

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Phthalate Ester Biodegradation In Agricultural Soil Using Exogenous Bacterial Consortium Under Fertilizer Addition, Theodora Mega Putri Jan 2022

Phthalate Ester Biodegradation In Agricultural Soil Using Exogenous Bacterial Consortium Under Fertilizer Addition, Theodora Mega Putri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Phthalate esters (PAEs) have been classified as a priority contaminant due to their endocrine-disrupting properties and abundance in the environment. Previously, several PAE-degrading consortia were enriched from landfill soil. This study aimed to initially select a consortium with efficient degradation activity, to characterize based on degradation kinetics and community dynamics, and to evaluate bioaugmentation feasibility while maintaining activity through continuous transfer. It was obtained based on enriched consortium selection that LF-NK-DEHP could simultaneously degrade 92.9% 100 mg l-1 DBP and 63.4% 100 mg l-1 DEHP within seven days. Kinetic degradation revealed DBP degradation by LF-NK-DEHP to fit the modified Gompertz …

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Effect Of Normal Saline In Different Concentration On Soft Tissue Healing Of Extraction Wound: A Clinical Study, Walailuk Kunthikarn Jan 2022

Effect Of Normal Saline In Different Concentration On Soft Tissue Healing Of Extraction Wound: A Clinical Study, Walailuk Kunthikarn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sodium chloride solution (NaCl) is regarded as the most suitable and recommended medical irrigation, because of the nontoxic and isotonic properties. The previous in vitro study showed that NaCl solution induced migration and extracellular matrix excretion from human gingival fibroblast. However, there is no report concerning the effect of rinsing extraction wound with NaCl solution in clinical trial study. This research study is split mouth single-blind randomized controlled clinical trial to investigate if rinsing with 0.9% and 1.8% NaCl solution on extraction wound can promote soft tissue healing. The operation was performed in 60 socket sites. The patients, who had …

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The Effects Of Decellularized Extracellular Matrix Derived From Jagged1-Treated Human Dental Pulp Stem Cells On Biological Responses Of Stem Cells Isolated From Apical Papilla, Suphalak Phothichailert Jan 2022

The Effects Of Decellularized Extracellular Matrix Derived From Jagged1-Treated Human Dental Pulp Stem Cells On Biological Responses Of Stem Cells Isolated From Apical Papilla, Suphalak Phothichailert

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective: Indirect Jagged1 immobilization efficiently activates canonical Notch signaling in hDPSCs. This study aimed to investigate the characteristic of the Jagged1-treated hDPSCs-derived dECM and its biological activity on odonto/osteogenic differentiation SCAPs. Methods: Bioinformatic database of Jagged1-treated hDPSCs was analyzed by NetworkAnalyst. hDPSCs seeded on Jagged1 immobilized surface were maintained with N or OM followed by decellularization procedure, dECM-N or dECM-OM, respectively. SCAPs were reseeded on each dECM with either normal medium or OM. Cell viability was determined by MTT assay. Characteristics of dECMs and SCAPs were evaluated by SEM, EDX, immunofluorescent staining and alcian blue staining. A Multipotential differentiation assay. …

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Effects Of The Orthodontic Miniscrew Geometric Design On The Bone Microdamage And Primary Stability, Panida Methawit Jan 2022

Effects Of The Orthodontic Miniscrew Geometric Design On The Bone Microdamage And Primary Stability, Panida Methawit

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study investigated the effects of the pitch (P) and thread shape on orthodontic miniscrew cortical bone microdamage. The relationship between microdamage and primary stability was examined. Ti6Al4V orthodontic miniscrews had custom-made thread heights (H) and pitch sizes and were classified into the following groups: HCPC (control); HCPN (with a narrower pitch); and HTPC (with a taller thread height) (HC = 0.12 mm; HT = 0.36 mm; PC = 0.60 mm; PN = 0.30 mm). The orthodontic miniscrews were inserted in the pilot hole of prepared 1.0-mm-thick tibia cortical bone, and the maximum insertion torque (MIT) and Periotest value (PTV) …

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Prediction Of Future Caries In Toddlers Via Salivary Microbiome: A 1-Year Longitudinal Study, Ratcha Raksakmanut Jan 2022

Prediction Of Future Caries In Toddlers Via Salivary Microbiome: A 1-Year Longitudinal Study, Ratcha Raksakmanut

Chulalongkorn University Theses and Dissertations (Chula ETD)

Dental caries in children is the most common chronic oral disease that could disturb their quality of life including their development. Early prevention is a key approach to reducing the prevalence of early childhood caries. However, a reliable caries predictor, as an essential tool for targeted prevention that is important to this approach, is still lacking for infants before caries onset. Therefore, we aimed to develop the caries risk prediction model based on the salivary microbiome of caries-free 1-year-old children to predict caries onset at 1-year follow-up. Using a nested case-control design within a prospective cohort study, 30 saliva samples, …

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Expression Of Superoxide Dismutase 3 (Sod3) During Orthodontic Tooth Movement In Rats, Phanchanit Jindarojanakul Jan 2022

Expression Of Superoxide Dismutase 3 (Sod3) During Orthodontic Tooth Movement In Rats, Phanchanit Jindarojanakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hypoxia induces reactive oxygen species (ROS) production in periodontal tissues. Superoxide dismutase 3 (SOD3) is an enzyme protecting cells from ROS. This study investigated SOD3 expression and function during rat orthodontic tooth movement (OTM) and in hypoxia-exposed rat PDL cells. OTM of right maxillary first molars were performed in Sprague-Dawley rats for 1 and 14 days (n = 6 per group). SOD3 and HIF-1α expression were evaluated by immunohistochemistry. SOD3 effects on cell viability and proliferation, ROS production, and mRNA expression of Hif-1α, Rankl, and Opg in PDL cells and osteoclast differentiation were investigated under normoxia and hypoxia. SOD3 expression …

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The Effect Of Nano-Silver Fluoride In Remineralization On Artificial Dentine Caries: An In Vitro Study, Peeraya Punpeng Jan 2022

The Effect Of Nano-Silver Fluoride In Remineralization On Artificial Dentine Caries: An In Vitro Study, Peeraya Punpeng

Chulalongkorn University Theses and Dissertations (Chula ETD)

The aim of this in vitro study was to compare the remineralization efficiency among Nano-silver fluoride 400 ppm (NSF400), 38% Silver diamine fluoride (38% SDF) and 5% Sodium fluoride (5% NaF) on artificial dentine caries in permanent molar or premolar teeth. The teeth were sectioned to obtain 120 specimens (3 x 3 x 2 mm3). After artificial caries were created, the lesion depth (LD) was measured using Micro-computed tomography (Micro-CT) (n=15 specimens/group), and the surface microhardness (SMH) was measured using a Knoop microhardness tester (n=15 specimens/group). Subsequently, all specimens were randomly assigned to four groups: Group 1) NSF400, Group 2) …

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Evaluation Of Different Scaffolds For Bone Regeneration In Rat Calvarial Bone Defects, Jeerawit Sirakittiworapong Jan 2022

Evaluation Of Different Scaffolds For Bone Regeneration In Rat Calvarial Bone Defects, Jeerawit Sirakittiworapong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Scaffold plays a key role in the context of tissue engineering. It not only provides a structural support for cells, but also creates an appropriate milieu for recruited cells, enhancing the therapeutic effects of cell-based treatments, and enabling the controlled release of biological cues such as growth factors. Collagen is widely used scaffold material in tissue engineering, particularly in periodontal and bone regeneration. Other natural polymers, however, have been developed and can be useful. Therefore, in this study, we aim to investigate the ability of four different natural polymers including collagen, chitosan, silk fibroin, and silk fibroin/gelatin hydrogel in promoting …

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Protein Expression After Gingival Injection Of Messenger Rna Encoding Platelet-Derived Growth Factor-Bb In Ligature-Induced Rat Periodontitis, Pimphorn Meekhantong Jan 2022

Protein Expression After Gingival Injection Of Messenger Rna Encoding Platelet-Derived Growth Factor-Bb In Ligature-Induced Rat Periodontitis, Pimphorn Meekhantong

Chulalongkorn University Theses and Dissertations (Chula ETD)

As a new class of medicines, mRNA platform has proven to be safe and effective. The objective of this study was to investigate the effect of local inflammation on platelet derived growth factor-BB (PDGF-BB) expression after gingival injection of PDGF-BB mRNA in lipid nanoparticles (LNPs) in ligature-induced rat periodontitis and to examine the dose-dependent effect of gingival delivery of mRNA. 3-0 silk was placed around maxillary left second molar for two weeks and removed, while the corresponding molar on the right was non-ligated. Under stereomicroscope, a significant bone loss at ligature sites was observed as compared to the non-ligature sites, …

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Application Of Core Annular Flow For Oil Transportation In Different Pipe Configurations Using Computational Fluid Dynamics, Cindy Dianita Jan 2022

Application Of Core Annular Flow For Oil Transportation In Different Pipe Configurations Using Computational Fluid Dynamics, Cindy Dianita

Chulalongkorn University Theses and Dissertations (Chula ETD)

The application of Core Annular Flow (CAF) has become an interesting solution in transporting heavy oil through pipeline because of its energy reduction and cost efficiency. Current study conducted a 3D computational fluid dynamic (CFD) to simulate CAF of oil-water in horizontal T- and Y-pipe junctions with two types of oil characteristic i.e., oil as Newtonian fluid and oil as non-Newtonian Carreau Fluid. The 2k factorial statistical experimental design was applied to investigate the effect of geometry on the flow performance. Eight cases were run with different diameter combinations and junction angle. The most attractive design was measured by the …

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Preparation Of Cellulose Micro- And Nano Fibers From Bagasse Through Deep Eutectic Solvent System, Worapoj Phuckpetch Jan 2022

Preparation Of Cellulose Micro- And Nano Fibers From Bagasse Through Deep Eutectic Solvent System, Worapoj Phuckpetch

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to prepare the cellulose micro- and nanofibers (CMNFs) using green chemistry viewpoint. The CMNFs were fabricated from sugarcane bagasse (SCB) pulp through treatment with zinc chloride (ZnCl2) - lactic acid (LA) in deep eutectic solvent (ZnCl2-LA DES) followed by short time ultrasonication of the DES-treated fibers in water. Under the optimum condition, the SCB pulp at high solid loading of 6.25% was treated with ZnCl2-LA DES. Lactate functionalized CMNFs with 230-270 nm length and 15-16 nm width were resulted in more than 90% yield and up to 62.2% crystallinity. The results showed that ZnCl2-LA DES was a …

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Stimulation Of Immune Response In Mice Using The Plant-Produced Sars-Cov-2 S1 Subunit Protein Linked To The Fc Region, Chalisa Panapitakkul Jan 2022

Stimulation Of Immune Response In Mice Using The Plant-Produced Sars-Cov-2 S1 Subunit Protein Linked To The Fc Region, Chalisa Panapitakkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 made a devastating impact worldwide, and virus management is challenging owing to a lack of specific therapies or affordable vaccines, especially in low-and middle-income countries. Hence a low-cost and efficient vaccine is necessary. In the present study, the immunogenicity of the plant-produced S1 subunit protein of SARS-CoV-2 was investigated in order to utilize it as a subunit vaccine. The SARS-CoV-2 S1 subunit protein was fused with the Fc region of human immunoglobulin (IgG1) and expressed in tobacco plants in species of Nicotiana benthamiana plants by agroinfiltration. The …

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Identification Of Potential Drug Targets For Covid 19 Patients With Cytokine Storm, Bayu Cakra Buana Jan 2022

Identification Of Potential Drug Targets For Covid 19 Patients With Cytokine Storm, Bayu Cakra Buana

Chulalongkorn University Theses and Dissertations (Chula ETD)

The outcome of SARS-CoV-2 infection may vary depending on gender, lifestyle, and age. The elderly is more likely to have the severe condition than the younger population. However, several young people suffer from severe lung diseases caused by cytokine dysregulation, also known as a "cytokine storm." Cytokine storms are associated with Covid-19 severity and mortality. Identification of potential cytokine storm targets is critical for improving patient survival. Transcriptomic analysis of expression data from publicly available databases could be used to identify differentially expressed genes and pathways. The list of both results was used to narrow down the potential candidates in …

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Renieramycin T Derivative Dh_22 Induces Apoptosis In Lung Cancer Cells By Downregulation Of C-Myc Protein, Indiana Gita Anggraeni Jan 2022

Renieramycin T Derivative Dh_22 Induces Apoptosis In Lung Cancer Cells By Downregulation Of C-Myc Protein, Indiana Gita Anggraeni

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lung cancer is a prevalent cause of death, indicated by an elevated c-Myc level. We synthesized a new derivative of renieramycin T (RT), named DH_22, and tested it for anti-cancer activities in human lung cancer cells. In the cell viability and nuclear staining tests, DH_22 was discovered to be cytotoxic, with an IC50 of around 13 μM; and found to mediate apoptosis in the lung cancer cells. Meanwhile, in a mechanistic sense, DH_22 contributed to the activation of p53-dependent apoptosis and decreased the cellular level of c-Myc. The p53-dependent mechanism was indicated by an increase of p53, an induction of …

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Development Of Surrogate Sars-Cov-2 Virus Neutralization Test Method Using Plant-Produced Recombinant Proteins, Perawat Jirarojwattana Jan 2022

Development Of Surrogate Sars-Cov-2 Virus Neutralization Test Method Using Plant-Produced Recombinant Proteins, Perawat Jirarojwattana

Chulalongkorn University Theses and Dissertations (Chula ETD)

Detecting immunity against SARS-CoV-2 is vital for evaluating vaccine response and natural infection, but a conventional virus neutralization test (cVNT) requires BSL3 and live viruses, and a pseudo-virus neutralization test (pVNT) needs specialized equipment and trained professionals in BSL2. The surrogate virus neutralization test (sVNT) was developed to overcome these limitations. This study explored the use of angiotensin converting enzyme (ACE2) produced from Nicotiana benthamiana for the development of an affordable neutralizing antibodies detection assay. The results showed that the plant-produced ACE2-His can bind to the receptor binding domain (RBD) of the SARS-CoV-2, and was used to develop sVNT with …

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Development Of Plant-Produced Subunit Vaccines To Elicit The Immunogenicity Against Sars-Cov-2 Variant Viruses, Narach Khorattanakulchai Jan 2022

Development Of Plant-Produced Subunit Vaccines To Elicit The Immunogenicity Against Sars-Cov-2 Variant Viruses, Narach Khorattanakulchai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Since the emergence of the coronavirus pandemic in 2019 (COVID-19), the development of effective vaccines to combat the infection has accelerated worldwide. While the severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) with mutations in the receptor-binding domain (RBD) with high transmissibility, enhanced infectivity, and immune escape from vaccination is also predominantly emerging. Effective vaccines against variant of concern (VOC) and optimized booster vaccination strategies are thus highly required. Here, the gene encoding seven different RBD (ancestral (Wuhan), Alpha, Beta, Gamma, Kappa, Delta, and Epsilon variants) fused with the fragment crystallizable region (Fc) of human IgG1 (RBD-Fc) was constructed and cloned into …

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Predictors Of Fertility Quality Of Life In Infertile Patients Visiting Infertility Center In Kathmandu, Nepal : A Cross-Sectional Study., Shital Shakya Jan 2022

Predictors Of Fertility Quality Of Life In Infertile Patients Visiting Infertility Center In Kathmandu, Nepal : A Cross-Sectional Study., Shital Shakya

Chulalongkorn University Theses and Dissertations (Chula ETD)

In Nepal, infertility is a rising reproductive health issue with an estimated prevalence of 15%. There are few studies on quality of life in infertile women, but none on quality of life in infertile men and women although infertility is a shared condition and has effects on couples. The study objective is to describe the various predictors of infertile patients seeking infertility treatment and find association between these predictors and the quality of life. A cross-sectional study using a self-administered disease specific FertiQoL questionnaire was conducted among 409 infertile men and women seeking infertility treatment in an infertility center in …

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Economic Burden Of Snakebite And Cost-Effectiveness Of Improving Access To Snake Antivenom In Asean Countries, Chanthawat Patikorn Jan 2022

Economic Burden Of Snakebite And Cost-Effectiveness Of Improving Access To Snake Antivenom In Asean Countries, Chanthawat Patikorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

The Association of Southeast Asian Nations (ASEAN) countries are among the tropical regions with disproportionately high incidence of snakebite. Understanding the snakebite and antivenom market situation and burden of snakebite is crucial for developing evidence-based strategies to pursue the goal set by the World Health Organization (WHO) to halve morbidity and mortality of snakebite by 2030. Firstly, we systematically review 23 cost of illness studies and 3 economic evaluations. Economic burdens of snakebite were underestimated and not extensively studied. Majority of studies only provided direct costs of snakebite patients presented to the hospitals. Thus, hospital data should be used to …

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Effectiveness Of Urban Childcare Center Indoor Air Quality Management Program (Ucc-Iaq) On Building Related Symptoms Among Care Takes :A Quasi Experimental Study, Sudkhed Detpetukyon Jan 2022

Effectiveness Of Urban Childcare Center Indoor Air Quality Management Program (Ucc-Iaq) On Building Related Symptoms Among Care Takes :A Quasi Experimental Study, Sudkhed Detpetukyon

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study investigates the effectiveness of the UCC-IAQ program in Thai childcare centers, focusing on BRS and indoor air pollution. It involves 81 childcare workers in Nonthaburi and Saraburi provinces, Over eight hours, air quality measurements were taken at ten settings. Data on BRS and building-related factors were collected via surveys. Statistically significant differences in pollution levels were not observed between the UCC-IAQ program's intervention and comparison groups. However, the intervention group experienced a significant decrease in BRS compared to the comparison group. Caretakers in rooms with rapid temperature variations had a 10.72 times higher likelihood of experiencing BRS (Adjusted …

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A Comparison Of Gender Equality Perception In Workplace Among Different Gender-Type Sport Federations In Indonesia, Risha Intan Rachmawati Jan 2022

A Comparison Of Gender Equality Perception In Workplace Among Different Gender-Type Sport Federations In Indonesia, Risha Intan Rachmawati

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to compare the perception of gender equality between male and female staff across three different sport federations. Sports have been stereotyped with genders for decades. In this thesis, three different sport federations were selected as a representative of different genders where football was a representative of masculine, gymnastic was a representative of feminine, and badminton was a representative of neutral. Not only were these three sport federations stereotyped with genders, these three sports were also very famous among Indonesian population. The study employed a mixed-method research design where a survey and an in-depth interview were used. 390 …

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The Effects Of Co-Ingestion Of Highly Branched Cyclic Dextrin And Dietary Nitrate On Physiological Responses And Endurance Capacity In Recreational Endurance Runners, Songdhasn Chinapong Jan 2022

The Effects Of Co-Ingestion Of Highly Branched Cyclic Dextrin And Dietary Nitrate On Physiological Responses And Endurance Capacity In Recreational Endurance Runners, Songdhasn Chinapong

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to investigate the effects of co-ingestion of HBCD and dietary nitrate on physiological responses and endurance capacity in recreational endurance runners compared with an isocaloric HBCD beverage, a maltodextrin-dietary nitrate beverage, and a maltodextrin beverage during high-intensity prolong running. Nine male marathon runners (age 35.00 ± 4.00 years) participated in a double-blind crossover design, where they were randomly assigned to receive either the co-ingesting 1.5 g HBCD·kg-1 BM and 500 mg dietary NO3- (~8.00 mmol NO3-) beverages (HBCD+NO3-), the ingesting 1.5 g HBCD·kg-1 BM (HBCD), the co-ingesting 1.5 g MD·kg-1 BM and 500 mg dietary NO3- (~8.00 …

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Multi-Output Learning For Predicting Evaluation And Reopening Of Github Pull Requests On Open-Source Projects, Peerachai Banyongrakkul Jan 2022

Multi-Output Learning For Predicting Evaluation And Reopening Of Github Pull Requests On Open-Source Projects, Peerachai Banyongrakkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

GitHub's pull-based development model is widely used by software development teams to manage software complexity. Contributors create pull requests for merging changes into the main codebase, and integrators review these requests to maintain quality and stability. However, a high volume of pull requests can overburden integrators, causing feedback delays. Previous studies have used machine learning and statistical techniques with tabular data as features, but these may lose meaningful information. Additionally, acceptance and latency may not be sufficient for the pull request evaluation. Moreover, reopened pull requests can add maintenance costs and burden already-busy developers. This thesis proposes a novel multi-output …

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Innovation Process Of Amorphous Cellulose - Graphene Oxide Hybrid Structure For Water Treatment In A Shrimp Farm, Kongkiat Phuphantrakun Jan 2022

Innovation Process Of Amorphous Cellulose - Graphene Oxide Hybrid Structure For Water Treatment In A Shrimp Farm, Kongkiat Phuphantrakun

Chulalongkorn University Theses and Dissertations (Chula ETD)

Amorphous cellulose-graphene oxide bead composites are popularly employed in water purification. However, the existing fabrication methods of amorphous cellulose (AC) and amorphous cellulose-graphene oxide (ACGO) beads are complicated with many chemical use, multi-step, time and energy-consuming. In this research, we proposed an efficient method for fabricating amorphous cellulose-graphene oxide (ACGO) beads using less chemical under a simple 2-step approach. The production process of AC and ACGO beads was successfully fabricated via sulfuric acid (H2SO4) gelatinization and regeneration using eucalyptus paper as a raw material. The cellulose gel was droplet-extruded into deionized (DI) water and transformed into a solid bead via …

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Challenges Of Ngos In Facilitating Access To Health Care Services For The Urban Poor In Slums During The Covid-19 Pandemic: A Case Study Of Kampala, Uganda||ความท้าทายขององค์กรนอกภาครัฐในการช่วยเหลือการเข้าถึงบริการการดูแลสุขภาพสำหรับคนจนเมืองในชุมชนแออัดในช่วงการระบาดของโรคติดเชื้อไวรัสโคโรนา 2019 กรณีศึกษา กรุงกัมปาลา ประเทศยูกันดา, Brendah Kyaterekera Jan 2022

Challenges Of Ngos In Facilitating Access To Health Care Services For The Urban Poor In Slums During The Covid-19 Pandemic: A Case Study Of Kampala, Uganda||ความท้าทายขององค์กรนอกภาครัฐในการช่วยเหลือการเข้าถึงบริการการดูแลสุขภาพสำหรับคนจนเมืองในชุมชนแออัดในช่วงการระบาดของโรคติดเชื้อไวรัสโคโรนา 2019 กรณีศึกษา กรุงกัมปาลา ประเทศยูกันดา, Brendah Kyaterekera

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study investigates the challenges NGOs face in providing healthcare access to the urban poor during the COVID-19 pandemic, using cases of slum communities in Kampala, the capital city of Uganda. The COVID-19 pandemic has disproportionately affected vulnerable populations, including those living in slums characterized by overcrowding, inadequate infrastructure, and limited access to basic services, especially access to healthcare, a fundamental right of people. During the past years, the pandemic has further restricted the already limited accessibility to healthcare services for the urban poor. The primary research methodology employed in this study is case study research, using in-depth interviews, observations, …

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Role Of Kca3.1 Channel On Renal Fibrosis In Doxorubicin-Induced Feline Kidney Cell Line, Penpicha Kuedbantakien Jan 2022

Role Of Kca3.1 Channel On Renal Fibrosis In Doxorubicin-Induced Feline Kidney Cell Line, Penpicha Kuedbantakien

Chulalongkorn University Theses and Dissertations (Chula ETD)

Feline renal fibrosis is the most common outcome of chronic kidney disease in cats. Nowadays, there is no specific treatment and the mechanism of fibrosis in cats is still unknown. Intermediate conductance Ca2+ activated K+ channel (KCa3.1) plays an important role in novel therapy for organ fibrosis in many species. Transforming growth factor- β (TGF-β)/Smad signaling pathway and KCa3.1 has been shown to relate to renal fibrosis. This study aimed to investigate the relationship between KCa3.1 and feline renal fibrosis via TGF-β/Smad signaling pathway. CRFK cultures were induced with doxorubicin (DOX) to generate feline renal fibrosis model. Triarymethane-34 (TRAM-34), selective …

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Effects Of Microplastics On Gene Expression To Nonspecific Immune System In Pacific White Shrimp (Litopenaeus Vannamei)., Songsak Niemcharoen Jan 2022

Effects Of Microplastics On Gene Expression To Nonspecific Immune System In Pacific White Shrimp (Litopenaeus Vannamei)., Songsak Niemcharoen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Microplastics pollution effects are increasingly observed as new studies emerge in different ecosystem and organismal levels. It is becoming clear that exposure to different types of microplastic can impair the nonspecific immune system of crustaceans. Therefore, this study investigated the impact of high-density polyethylene microplastic particles (HDPE-MP) in feed on nonspecific immune system gene expression of Pacific white shrimp (Litopenaeus vannamei). The LC50 at day 28 of HDPE-MP ingestion was 3.074% w/w of food. Superoxide dismutase (SOD) and the Glutathione peroxidase (GPx) gene were upregulated significantly in groups supplemented with 0.1 and 0.5% of HDPE-MP and then down-regulated significantly after …

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Evolution And Phylogenetic Studies Of Porcine Epidemic Diarrhea Virus And Porcine Deltacoronavirus In Thailand From 2008 To 2021, Christopher James Stott Jan 2022

Evolution And Phylogenetic Studies Of Porcine Epidemic Diarrhea Virus And Porcine Deltacoronavirus In Thailand From 2008 To 2021, Christopher James Stott

Chulalongkorn University Theses and Dissertations (Chula ETD)

PEDV and PDCoV are recognized as important pathogens causing enteric disease in swine herds worldwide. During 2014-2015, several outbreaks of PEDV occurred in many provinces in Thailand, and the clinical signs were more severe than those of classical PEDV in Thailand during 2008-2012. Therefore, the new introduction of PEDV or mutated virus strains was suspected. In 2015, PDCoV was also reported in Thailand, making the situation of enteric disease in the Thai swine industry worse. Since genetic classification studies of PEDV and PDCoV had never been done in Thailand before, this study aimed to investigate the genetic diversity of PEDV …

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Correlation Of The Classical Swine Fever (Csf) Antibody Levels Detected By Serum Neutralizaton And Enzyme-Linked Immunosorbent Assay (Elisa), Chairani Ridha Maghfira Jan 2022

Correlation Of The Classical Swine Fever (Csf) Antibody Levels Detected By Serum Neutralizaton And Enzyme-Linked Immunosorbent Assay (Elisa), Chairani Ridha Maghfira

Chulalongkorn University Theses and Dissertations (Chula ETD)

Classical swine fever is an important viral disease that has a devastating impact on the swine industry. The Enzyme-linked immunosorbent assay (ELISA) provides a simpler and more practical approach to detect classical swine fever virus (CSFV) antibodies compared to the serum neutralization (SN) test. However, antibody responses detected by ELISA cannot directly exhibit a protective level. This study aimed to evaluate the correlation of classical swine fever antibody responses detected by SN assay and commercial ELISA. A total of 522 negative and positive serum samples were tested by the SN and ELISA. Correlation, an agreement between two assays, and comparisons …

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Establishment Of Transplantation Platform For Delivering Mouse Induced Pluripotent Stem Cell-Derived Insulin-Producing Cells (Mips-Ipcs) For Diabetes Treatment, Hong Thuan Tran Jan 2022

Establishment Of Transplantation Platform For Delivering Mouse Induced Pluripotent Stem Cell-Derived Insulin-Producing Cells (Mips-Ipcs) For Diabetes Treatment, Hong Thuan Tran

Chulalongkorn University Theses and Dissertations (Chula ETD)

Pancreatic beta-cell replacement is recognized for feasible type 1 diabetes (T1D) treatment. However, in post-transplantation, the autoimmune destruction incidentally attacks the activity and survival of beta-cells are reported in animal and human. To address these concerns, the generation of immortalized, biocompatible beta-cells, and the engraftment platform are insightfully investigated. The stepwise chemical process was used for in vitro Insulin-producing cells (IPCs) production from mouse gingival fibroblast-induced pluripotent stem cells (mGF-iPSCs). The real-time qRT-PCR, glucose stimulation C-peptide/Insulin secretion, immunostaining, and visible cell methods were examined during IPC differentiation. The encapsulated-IPC beads were loaded into subcutaneous pocket space via transplantation platform. Completed …

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