Digital Commons Network^™

Fight Fire With Fire: The Need For A Vaccine Based On Intrinsic Disorder And Structural Flexibility, Vladimir N. Uversky

Molecular Medicine Faculty Publications

The absence of advancement in finding efficient vaccines for several human viruses, such as hepatitis C virus (HCV), human immunodeficiency virus type 1 (HIV-1), and herpes simplex viruses (HSVs) despite 30, 40, and even 60 years of research, respectively, is unnerving. Among objective reasons for such failure are the highly glycosylated nature of proteins used as primary vaccine targets against these viruses and the presence of neotopes and cryptotopes, as well as high mutation rates of the RNA viruses HCV and HIV-1 and the capability to establish latency by HSVs. However, the lack of success in utilization of the structure-based …

Specific S100 Proteins Bind Tumor Necrosis Factor And Inhibit Its Activity, Alexey S. Kazakov, Marina Y. Zemskova, Gleb K. Rystsov, Alisa A. Vologzhannikova, Eugenia I. Deryusheva, Victoria A. Rastrygina, Andrey S. Sokolov, Maria E. Permyakova, Ekaterina A. Litus, Vladimir N. Uversky, Eugene A. Permyakov, Sergei E. Permyakov

Molecular Medicine Faculty Publications

Tumor necrosis factor (TNF) inhibitors (anti-TNFs) represent a cornerstone of the treatment of various immune-mediated inflammatory diseases and are among the most commercially successful therapeutic agents. Knowledge of TNF binding partners is critical for identification of the factors able to affect clinical efficacy of the anti-TNFs. Here, we report that among eighteen representatives of the multifunctional S100 protein family, only S100A11, S100A12 and S100A13 interact with the soluble form of TNF (sTNF) in vitro. The lowest equilibrium dissociation constants (Kd) for the complexes with monomeric sTNF determined using surface plasmon resonance spectroscopy range from 2 nM to 28 nM. The …

Mechanism Of Phase Separation In Aqueous Two-Phase Systems, Amber R. Titus, Pedro P. Madeira, Luisa A. Ferreira, Vladimir Y. Chernyak, Vladimir N. Uversky, Boris Y. Zaslavsky

Molecular Medicine Faculty Publications

Liquid-liquid phase separation underlies the formation of membrane-less organelles inside living cells. The mechanism of this process can be examined using simple aqueous mixtures of two or more solutes, which are able to phase separate at specific concentration thresholds. This work presents the first experimental evidence that mesoscopic changes precede visually detected macroscopic phase separation in aqueous mixtures of two polymers and a single polymer and salt. Dynamic light scattering (DLS) analysis indicates the formation of mesoscopic polymer agglomerates in these systems. These agglomerates increase in size with increasing polymer concentrations prior to visual phase separation. Such mesoscopic changes are …

Trapped Surfaces, Topology Of Black Holes, And The Positive Mass Theorem, Lan-Hsuan Huang, Dan A. Lee

Publications and Research

No abstract provided.

Satellite Evidence Of Canopy-Height Dependence Of Forest Drought Resistance In Southwestern China, Peipei Xu, Wei Fang, Tao Zhou, Hu Li, Xiang Zhao, Spencer Berman, Ting Zhang, Chuixiang Yi

Publications and Research

The frequency and intensity of drought events are increasing with warming climate, which has resulted in worldwide forest mortality. Previous studies have reached a general consensus on the size-dependency of forest resistance to drought, but further understanding at a local scale remains ambiguous with conflicting evidence. In this study, we assessed the impact of canopy height on forest drought resistance in the broadleaf deciduous forest of southwestern China for the 2010 extreme drought event using linear regression and a random forest (RF) model. Drought condition was quantified with standardized precipitation evapotranspiration index (SPEI) and drought resistance was measured with the …

An Optimal Lysis Time Maximizes Bacteriophage Fitness In Quasi-Continuous Culture, Sherin Kannoly, Abhyudai Singh, John J. Dennehy

Publications and Research

Optimality models have a checkered history in evolutionary biology. While optimality models have been successful in providing valuable insight into the evolution of a wide variety of biological traits, a common objection is that optimality models are overly simplistic and ignore organismal genetics. We revisit evolutionary optimization in the context of a major bacteriophage life history trait, lysis time. Lysis time refers to the period spanning phage infection of a host cell and its lysis, whereupon phage progenies are released. Lysis time, therefore, directly determines phage fecundity assuming progeny assembly does not exhaust host resources prior to lysis. Noting that …

Internal Combustion Engines: Modeling Internal Temperature As A Function Of Time, Garrett Fandrich

Undergraduate Journal of Mathematical Modeling: One + Two

Just like any thermodynamic system, combustion engines must be cooled to eliminate friction due to heat. Without proper cooling, internal components, such as connecting rods, rod bearings, and pistons can be severely damaged due to thermal expansion, leading to severe damage to the engine block or outright catastrophic failure. Modern engines are cooled using coolant, which flows through internal passageways within the engine block to pull heat away from the system. The use of coolant and external components, such as a water pump, radiator, and thermostat allow an engine to efficiently warm to standard operating temperature and remain at said …

Bio-Inspired Sutures: Using Finite Element Analysis To Parameterize The Mechanical Response Of Dovetail Sutures In Simulated Bending Of A Curved Structure, Melissa M. Gibbons, Diana A. Chen

School of Engineering: Faculty Scholarship

Many animals have protective anatomical structures that allow for growth and flexibility; these structures contain thin seams called sutures that help the structure to absorb impacts. In this study, we parameterized the stiffness and toughness of a curved archway structure based on three geometric properties of a suture through finite element, quasi-static, three-point bending simulations. Each archway consisted of two symmetric pieces linked by a dovetail suture tab design. The three parameters included suture tab radii (1–5 mm), tangent lengths (0–20 mm), and contact angles (0–40◦). In the simulations, a steel indenter was displaced 6.5 mm to induce progressive tab …

Covid-19 And Diabetes, Radhika Desai

Honors Undergraduate Theses

Patients with Type 2 Diabetes (T2D) have been found to have increased mortality and morbidity for COVID-19 and are at higher risk for severe disease once infected with COVID-19. Clearly, there exists a relationship between T2D and COVID-19 that requires more attention. In order to understand the mechanisms by which T2D contributes to more severe COVID-19 disease, attention was turned to extracellular vesicles (EVs). It was speculated that viral RNA components of the COVID-19 virus may have originated from circulating EVs that came from infected cells and use a Trojan Exosome method to infect host cells. It is necessary to …

Concerning The Authority Of A Licensed Psychologist To Prescribe Psychotropic Medication For The Treatment Of Mental Health Disorders, Colorado General Assembly

Session Laws 2001-Present

No abstract provided.

The Grave Barrier Reef, Savannah J. Szamborski

GLO 100 Introduction to Global Studies: Policy Analysis Papers

The Great Barrier Reef, located off the Northern East coast of Australia, has been experiencing significant levels of coral bleaching since 1998 when it experienced its first major bleaching. When water temperatures rise due to climate change, corals lose their bright colors and turn white. Once a coral is bleached, it becomes more vulnerable to other factors, increasing its chance of mortality. Greenhouse gas emissions have been seen to be one of the major factors that lead to climate change. The Australian and Queensland governments have put many different acts and policies into place to protect the Great Barrier Reef …

Contesting Imagined Communities: Minor Feelings, Opacity, And Spectrality In Ling Ma’S Severance, Christine Marks

Publications and Research

This article foregrounds pandemic fiction’s subversive and visionary potential through the example of Ling Ma’s dystopian pandemic novel Severance (2018). Emphasizing fiction’s capacity for symbolic intervention in a pandemic era, I argue that the text undermines tropes of nation-building through strategic employment of minor feelings, opacity, and spectral liminality. My analysis focuses on the Chinese American protagonist’s conflicted relationship with imagined communities forged in the conventional “outbreak narrative” (Wald) as her unhomely positionality places her at odds with unifying ideological responses to the catastrophic upheaval of the pandemic. At the intersection of pandemic and diasporic literature, Ma’s novel constitutes a …

Active Learning And Student Achievement: A Matter Of Space, Experiences, Or Pedagogy?, Justin Bruner, Rebecca Affoo, Angela M. Dietsch

Department of Special Education and Communication Disorders: Faculty Publications

The goal of this study was to contribute to research on active learning by addressing the problem of disentangling the effects of classroom architecture, student characteristics, and pedagogical design as they relate to student achievement. The study utilized a quasi-experimental design where data was collected on student perceptions of their classroom, their experience in the course, and the pedagogy of the instructor, then analyzed with respect to the course grade. Results indicate that neither student perceptions of the classroom spaces nor the spaces themselves had an impact on course grade, but the pedagogy employed by the instructor and student experiences …

The Effect Of Taste On Swallowing: A Scoping And Systematic Review, Rachel Mulheren, Ross M. Westemeyer, Angela M. Dietsch

Department of Special Education and Communication Disorders: Faculty Publications

Consuming foods and liquids for nutrition requires the coordination of several muscles. Swallowing is triggered and modified by sensory inputs from the aerodigestive tract. Taste has recently received attention as a potential modulator of swallowing physiology, function, and neural activation; additionally, taste impairment is a sequela of COVID-19. This review presents factors impacting taste and swallowing, systematically summarizes the existing literature, and assesses the quality of included studies. A search was conducted for original research including taste stimulation, deglutition-related measure(s), and human participants. Study design, independent and dependent variables, and participant characteristics were coded; included studies were assessed for quality …

“I Just Stay In The House So I Don’T Need To Explain”: A Qualitative Investigation Of Persons With Invisible Disabilities, Gillian Hendry, Claire Wilson, Mairi Orr, Rebecca Scullion

Department of Special Education and Communication Disorders: Faculty Publications

Research has shown that persons with disabilities continually face discrimination. More research attention has focused on individuals’ experiences of visible disability, despite evidence that there are higher numbers worldwide of people with invisible disabilities. As such, persons with invisible disabilities can feel under-represented in disability literature. A qualitative study was conducted to address this. Twenty-five persons with an invisible disability were recruited to take part in focus groups and interviews aimed at understanding the lived experience of invisible disability on social life and within the workplace. Data were analyzed using Braun and Clarke’s reflexive thematic analysis, identifying themes of (1) …

Digital Entrepreneurship: Context And Conceptualization, Kisito Futonge Nzembayie, Anthony Paul Buckley

Books/Book Chapters

Recognizing how the lines between digital and traditional forms of entrepreneurship are blurring, this forward-thinking book combines digital technology and entrepreneurship perspectives to advance knowledge on this paradigm-shifting typology of entrepreneurship.

Concerning Establishing A Minimum Three Day Waiting Period Prior To The Delivery Of A Purchased Firearm, Colorado General Assembly

Session Laws 2001-Present

No abstract provided.

Concerning Measures To Promote Reductions In Greenhouse Gas Emissions In Olorado And In Connection Therewith, Colorado General Assembly

Session Laws 2001-Present

No abstract provided.

Concerning The Procedure For Sealing Of Criminal Records For Nonviolent Offenses And In Connection Therewith Addressing Workforce Shortages, Colorado General Assembly

Session Laws 2001-Present

No abstract provided.

Concerning The Creation Of The Producer Responsibility Program For Statewide Recycling And In Connection Therewith, Colorado General Assembly

Session Laws 2001-Present

No abstract provided.

Predicting Munsell Color For Turfgrass Leaves, William L. Berndt, Roch E. Gaussoin

Department of Agronomy and Horticulture: Faculty Publications

Linking turfgrass color to hue, value, chroma (H V/C) in the Munsell Plant Tissue Color Book is a visual comparison process for specifying and communicating plant color. If subjectivity of visual comparison can be mitigated, then accuracy of color matching may be improved. Research was conducted to develop an algorithm predicting H V/C from CIE-xyY color (xyY) in digital images of leaves of four turfgrasses. First, value-chroma (V/C) arrays for Munsell hue groups 5Y, 2.5GY, 5GY, 7.5GY, 10GY, and 2.5G were converted to xyY. Next, chromaticity (xy) plots from each array were fitted with …

Regenerating Agroecosystems By Overcoming Human Exceptionalism In Designing For Increased Equity Of Benefits From Ecoservices, Ali Loker, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Our commentary explores three critical issues related to ecosystem services. First is how ecoservices are currently designed and implemented primarily for human benefit without concern for how these impact other species. We conclude that awareness of this imbalance is the first step toward meaningful change. Second we observe that human exceptionalism guides most decisions, and ask whether we can overcome this mind-set to embrace ecoregeneration and design of resilient and mutually beneficial agroecosystems. Our attitude toward the challenge and moving toward greater humility about human roles that guide management decisions in the ecosystem is a requisite for change. Third we …

Adenoviral-Vectored Centralized Consensus Hemagglutinin Vaccine Provides Broad Protection Against H2 Influenza A Virus, Erika M. Petro-Turnquist, Brianna L. Bullard, Matthew J. Pekarek, Eric A. Weaver

Nebraska Center for Virology: Faculty Publications

Several influenza pandemics have occurred in the past century, one of which emerged in 1957 from a zoonotic transmission of H2N2 from an avian reservoir into humans. This pandemic caused 2–4 million deaths and circulated until 1968. Since the disappearance of H2N2 from human populations, there has been waning immunity against H2, and this subtype is not currently incorporated into seasonal vaccines. However, H2 influenza remains a pandemic threat due to consistent circulation in avian reservoirs. Here, we describe a method of pandemic preparedness by creating an adenoviral-vectored centralized consensus vaccine design against human H2 influenza. We also assessed the …

The Application Of The Skin Virome For Human Identification, Ema H. Graham, Jennifer Clarke, Samodha Fernando, Joshua Herr, Michael Adamowicz

Nebraska Center for Virology: Faculty Publications

The use of skin virome offers a unique approach for human identification purposes in instances where a viable and statistically relevant human DNA profile is unavailable. The skin virome may act as an alternative DNA profile and/or an additional form of probative genetic material. To date, no study has attempted to investigate the human virome over a time series across various physical locations of the body to identify its diagnostic potential as a tool for human identification. For this study, we set out to evaluate the stability, diversity, and individualization of the human skin virome. An additional goal was to …

Data Capitalism, Digital Health, And Marxism, Samantha C. Stanger

Communication Senior Capstones

Abstract

When immersed in a capitalist society individuals often fail to see the ways in which they are exploited, and when these discussions do come into discourse they are often geared toward workplace labor. Less commonly do we consider the ways in which these problems arise in conjunction with free labor, mainly performed in digital spaces. While less often considered or discussed, the exploitation occurring in the digital world is real and has substantial consequences, particularly when the digital spaces involve sensitive information and data. This paper aims to address how data capitalism functions in three different digital health spaces: …

The State, The Udhr, And The Social Construction Of Family In Human Rights: The Case Of The Scarborough 11, Abbey S. Willis, Mary C. Burke, Davita Silfen Glasberg

Societies Without Borders

The Universal Declaration of Human Rights (UDHR) (UN 1947:34) declares in Article 16(3) that “the family is the natural and fundamental group unit of society and is entitled to the full protection by society and the state.” However, the UDHR does not define family, but rather presumes it is defined by traditional heteronormative marriage in a nuclear family. The failure of the UDHR to consider a more expansive view of family leaves the definition of family centrally in the hands of the state, and affects the ability of all but traditional nuclear family forms to access other human rights. We …

Help Was Not On The Way: Intellectual Property Liability Relief In A Pandemic Era, Kim Vu-Dinh

Faculty Scholarship

On January 21, 2020, the United States recorded its first case of COVID-19. By April of that same year, numerous hospitals across the nation had exhausted entire reserves of personal protective equipment (PPE), with looming uncertainty as to when they would be replenished. As infection numbers increased exponentially, global demand for some types of PPE increased by 1000%.

Volunteers across the nation assembled teams of makers—some professionals, but also scores of amateurs—to craft the critical equipment needed to slow down the onslaught of the pandemic. From creating cloth masks to ventilator pistons, nonprofits and everyday citizens were able to partially …

Assessing The Influence Of Invasive Removal Techniques On Mangrove Growth And Burrowing Crab Populations, Liah Continentino

Theses & ETDs

Mangroves are unique ecosystems, bridging land and sea, and hosting a plethora of ecologically and economically valuable species. Mangroves provide a nursery habitat for marine life, protect and build shorelines, and sequester large amounts of atmospheric carbon. Mangrove trees have an interdependent relationship with the burrowing crabs which live alongside them. Burrowing crabs act as ecosystem engineers; assisting in nutrient cycling and decomposition, altering the sediment chemistry and promoting plant growth. This study takes place on a spoil mound caused by channelization in a mangrove island. Impacts of invasive tree removal techniques on mangrove growth, crab populations, and substrate characteristics …

“Therapy? What Am I, A Girl?”: Gender Differences In Willingness To Try Different Forms Of Therapy To Treat Anxiety, Samantha Forman

Theses & ETDs

Anxiety is an extremely prevalent mental disorder that can be treated in a multitude of ways. Both clinician-led therapies and minimal contact therapies have shown great efficacy in reducing anxiety symptomology. Factors that influence this efficacy include past experiences with therapy, opinions towards therapy, and individual levels of self-disclosure. Additionally, all of these factors have been shown to differ by the gender identity of the patient. The current study sought to explore if there were any gender differences in one’s willingness to try these different forms of therapy to help better manage their anxiety. The current study hypothesized that men …

The Heat Is On: An Analysis Of The Thermal Tolerance Of The Invasive Gecko Hemidactylus Mabouia As A Model For Ctmax Studies In Squamates, Sawyer Markham

Theses & ETDs

As climate change continues to threaten biodiversity around the world, time is running out to protect many species from extinction. Lizards are among the most threatened species, and over twenty percent of all lizards are expected to face extinction as temperatures increase globally. Hemidactylus mabouia is an invasive and highly abundant species of gecko in Florida and limited information is available on their thermal tolerance.These lizards were collected in Sarasota, Florida, where their CTmax was measured by the demonstration of loss of normal function in the form of pants and muscular spasms. I hypothesized that the two methods of measuring …