Digital Commons Network^™

Sequential Assembly Of 1-D And 2-D Origami Arrays, Nathan Shin

Theses, Dissertations and Capstones

DNA origami, invented by Paul Rothemund in 2006, provides many possible routes for the synthesis of complex macromolecular assemblies. This project involves the creation of 1-D and 2-D arrays of cross origami to support the development of DNA detection devices by providing precise platforms for these devices. The M13 plasmid is the most commonly used scaffold in DNA nanotechnology, limiting the size and complexity of an individual DNA origami construct. However, by creating larger assemblies, the dimensions can be multiplied. For example, by alternating two different types of cross origami, optical reporters can be separated by 200 nm, which is …

Mortality Risk Associated With Urban Land Use For Adult Eastern Diamondback Rattlesnakes (Crotalus Adamanteus), Mya Wiles

Theses, Dissertations and Capstones

Snakes have been a traditionally under-represented organism in urban ecology, but they face similar, if not greater challenges in the face of growing urban sprawl. Eastern diamondback rattlesnakes (Crotalus adamanteus, EDBs) are under consideration for listing under the Endangered Species Act due to population declines resulting from historical human persecution and habitat loss. This study used radio-telemetry data from a long-term monitoring project of adult EDBs on a developed sea island in South Carolina, USA. I reclassified a National Land Cover Dataset to reflect relative mortality risk for snakes attempting to move through the landscape. High-risk cover types …

Socioeconomic Determinants Of Antibiotic Resistance, Kausar Jama

Theses and Graduate Projects

Background: Antibiotics were first developed to treat wounded soldiers and cases of pneumonia in World War II. By the 1940s, antibiotics became widely used to treat several infectious processes. It was not long after antibiotics were being used frequently by clinicians that resistant strains began to emerge. Today, antibiotic resistance is a growing global health concern seen in many parts of the world. There are many factors that contribute to the prevalence of antibiotic resistance.

Purpose: This paper aims to demonstrate how socioeconomic factors play a role in the development and spread of antibiotic resistance. The literature review …

The Impact Of Conventional And Expanded Adverse Childhood Experiences (Aces) On Juvenile Offending Outcomes, Jesma C. Revering

Theses and Graduate Projects

Previous research has established a correlation between conventional adverse childhood experiences (ACEs) and juvenile offending. However, the possible moderating effect expanded ACEs has on this relationship remains unclear. Using the 2019 Minnesota Student Survey (MSS), the study included 278 youth from juvenile correctional facilities in Minnesota to examine the impact of conventional ACEs on juvenile offending, the impact of expanded ACEs on juvenile offending, and the impact of conventional ACEs on juvenile offending, moderated by expanded ACEs. Findings revealed a statistically significant positive effect of conventional ACEs on juvenile offending. Expanded ACEs had a significant negative relationship with juvenile offending. …

Washington University Magazine, August 2022

Washington University Magazine

No abstract provided.

Washington University Magazine, December 2022

Washington University Magazine

No abstract provided.

Comparing Efficacy Of Denosumab To Bisphosphonates In Fragility Fractures, Ronald Toledo Cardoso

Theses and Graduate Projects

Background: Osteoporosis is a disease of the bones with decreased bone mineral density and bone mass, leading to poor bone quality/structure, increasing the risk of fragility fractures. Pharmacological treatment is recommended to decrease risk of fractures. Current first line treatment is with bisphosphonates; however, alternative treatment includes denosumab. Both bisphosphonates and denosumab are antiresorptive drugs but differ in mechanism of action. Bisphosphonates remain as first line treatment.

Purpose: The purpose of this literature review is aimed at the PICO question in adults with osteoporosis, what is the efficacy of denosumab compared to bisphosphonates in decreasing the risk of …

What Alternative Models Are Effective In Improving Health Outcomes In Children With Obesity In The United States?, Kelsey Reineke

Theses and Graduate Projects

Background: Obesity in children has increased at an alarming rate in the past three decades and has been associated with multiple comorbidities. This literature review has been focused on pediatric obesity management research utilizing Family-Based Therapy, community-centered interventions, behavioral modifications, and implementations for management within the Primary Care setting.

Purpose: There has not been an established standard of care for children suffering from obesity. This investigation was focused on the question; What alternative models are effective in improving health outcomes in children with obesity in the United States?

Methods: A comprehensive literature review was conducted using Pub …

Thermal Ablation As An Alternative To Standard Breast Conserving Surgery In The Treatment Of Invasive Ductal Carcinoma Of The Elderly, Dani Ducharme

Theses and Graduate Projects

An aging population means an inevitable increase in cancer diagnoses, and thus, a novel solution is needed to address this looming problem. Within the field of breast cancer, thermal ablation has emerged as a promising alternative to surgical excision. As a minimally invasive treatment, it significantly reduces the morbidity and mortality for geriatric patients, as well as healthcare costs overall.

The purpose of this analysis is to understand the utility of thermal ablation for the treatment of invasive ductal carcinoma. Specifically in the context of elderly patients, it is determined if this treatment modality is superior to traditional breast conserving …

3d And Bio-Printing As An Efficient And Safe Alternative To Traditional Methods And Materials In Cardiovascular Diseases, Angela Asare-Ose

Theses and Graduate Projects

Background: Cardiovascular diseases are the leading cause of death globally. Therefore, there is the need for more resources to be poured into the research of more treatment options, diagnostic methods and preventative steps for patients. Though several technological advancements are being made in the world of medicine, it is not a bad idea to look at other avenues that have great potential to improve patient outcomes in cardiovascular diseases. 3D and bioprinting, though a relatively new technology in the world of medicine, is showing a promising future. It is already being applied in the field of orthopedic surgery and …

Greater Loss And Fragmentation Of Savannas Than Forests Over The Last Three Decades In Yunnan Province, China, R Sedricke Lapuz, Angelica Kristina M. Jaojoco, Sheryl Rose C. Reyes, Jose Don T. De Alban, Kyle W. Tomlinson

SOSE Affiliate: Manila Observatory

Yunnan Province, southwest China, has a monsoonal climate suitable for a mix of fire-driven savannas and fire-averse forests as alternate stable states, and has vast areas with savanna physiognomy. Presently, savannas are only formally recognised in the dry valleys of the region, and a no-fire policy has been enforced nationwide since the 1980s. Misidentification of savannas as forests may have contributed to their low protection level and fire-suppression may be contributing to vegetation change towards forest states through woody encroachment. Here, we present an analysis of vegetation and land-use change in Yunnan for years 1986, 1996, 2006, and 2016 by …

Preparation For General Chemistry, Jose Cobo

Open Educational Resources

No abstract provided.

Globe And Natural Inquirer Crosswalk Ms/Hs: Studying Water Quality With Middle School Students, Pam Evans, Globe U.S. Coordination Office

Teacher Resources

No abstract provided.

Development Of Carbohydrate-Based Antibiotics And Metabolic Chemical Reporters, Courtney Kondor

Wayne State University Dissertations

CHAPTER 1: Multidrug-resistant bacteria is a threat to public health and there is a need for the development of new antibiotics to fight bacterial resistance. Aminoglycoside antibiotics are attractive to researchers due to their lack of known drug related allergies, low cost, and broad spectrum of activity; however, they can cause nephrotoxicity and ototoxicity in patients. We modified the 2-deoxystreptamine ring of paromomycin to make the drug more flexible and potentially more selective for the bacterial ribosomal A-site to circumvent off-target effects that cause ototoxicity. However, we found that our compound, paromomexin, was significantly less active than paromomycin in all …

The Arsenic Induced Endoplasmic Reticulum Stress Response Via The Activating Transcription Factor 6 In Human Bronchial Epithelial Beas-2b Cells, Priya Wadgaonkar

Wayne State University Dissertations

Arsenic is a well-known human carcinogen associated with a number of cancers, including lung cancers. We have previously shown that long-term exposure to an environmentally relevantconcentration of inorganic arsenic (As3+) leads to the malignant transformation of the BEAS-2B cells, and some of the transformed cells show cancer stem-like features (CSCs) with a significant upregulation of glycolysis and downregulation of mitochondrial oxidative phosphorylation. In the present report, we investigate the short-term effect of As3+ on the endoplasmic reticulum (ER) stress response—the “unfolded protein response (UPR)” and metabolism in human bronchial epithelial cell line BEAS-2B cells. Treatment of the cells with inorganic …

Anaerobic Digestion Of Cereal Rye Cover Crop, Liangcheng Yang, Lucas D. Lamont, John C. Sedbrook, Nicholas J. Heller, David E. Kopsell

Faculty Publications - Agriculture

The rapid growth of cover crop planting area in the U.S. helps with erosion control, soil health, control of greenhouse gases, and also provides abundant biomass for the production of bioenergy and bioproducts. Given the cover crops’ compositional heterogeneity and variability, a tolerate platform technology such as anaerobic digestion (AD) is preferred but has not been widely used for cover crop biorefining. This study evaluated the biogas and methane yields from six cereal rye (Secale cereale L.) cover crops grown in the Midwest, using both bench- and pilot-scale anaerobic digesters. The effects of two critical factors, the total solids (TS) …

The Epistemology Of The Internet And The Regulation Of Speech In America, Brian Leiter

Articles

The Internet is the epistemological crisis of the 21st century: it has fundamentally altered the social epistemology of societies with relative freedom to access it. Most of what we think we know about the world is due to reliance on epistemic authorities, individuals, or institutions that tell us what we ought to believe about Newtonian mechanics, evolution by natural selection, climate change, resurrection from the dead, or the Holocaust. The most practically fruitful epistemic norm of modernity, empiricism, demands that knowledge be grounded in sensory experience, but almost no one who believes in evolution by natural selection or the reality …

Town Of Raymond, New Hampshire 2021 Annual Report., Raymond Town Representatives

Raymond, NH Annual Reports

This is an annual report containing vital statistics for a town/city in the state of New Hampshire.

Stem Teaching And Learning In Rural Communities: Exploring Challenges And Opportunities An Introduction To Volume 12, Issue 2 Of Tpre, Janet Stramel, Earl Legleiter

Teacher Education Faculty Publications

This special issue of Theory & Practice in Rural Education highlights STEM Teaching and Learning in Rural Communities. The articles selected represent both theory and practice and explore the complexities, practices, challenges, and opportunities facing rural schools and universities as they design and implement STEM teaching and learning. Articles from the field have related rural school success stories of how rural districts have overcome challenges to have effective and rich STEM teaching and learning in rural schools. Article submissions crossed a variety of topics, and three main themes emerged throughout the articles: (1) making STEM teaching relevant, (2) promising practices, …

Spatial And Temporal Variability Of Mercury In Upper And Lower Red Lake Walleye, Tyler Orgon

Biology Graduate Theses

Mercury is a global pollutant that is released into our environment by natural and anthropogenic processes resulting in extensive studies conducted on mercury cycling in aquatic ecosystems which has led to the issuance of human-health-based fish-consumption advisories. We examined total mercury concentrations in Walleye Sander vitreus from Upper and Lower Red Lakes, located in north central Minnesota, between 2019 and 2020. Upper and Lower Red Lake form a contiguous water body consisting of two large (483.1 and 665.6 km2, respectively) basins that are naturally connected by a 1.4 km-wide strait. Both basins are important fisheries for recreation, subsistence, and commercial …

Hsfa1d Regulates The Kinetics Of Heat-Induced Hsp17.6 Expression In Arabidopsis, Erin J. Kast , '15, Nicholas J. Kaplinsky

Biology Faculty Works

Arabidopsis contains four HSFA1 heat shock transcription factors (HSFA1a, HSFA1b, HSFA1d, and HSFA1e) that regulate the primary response to high temperature stress responses. These genes have overlapping functions and, while double and triple HSFA1 mutants have thermotolerance phenotypes, these genes have no reported single mutant thermotolerance phenotypes. We used an automated fluorescence microscopy system to quantitate the expression of a HSP17.6:GFP reporter with high temporal resolution to show that HSFA1d is required for normal heat-induced HSP17.6 expression. HSP17.6 expression is reduced and delayed in hsfa1d-1 mutants. This finding highlights the power of using gene …

Memphis State Magazine, 06:04, 1987 Fall

Memphis State University Magazine

Cover Story, "Weighty Fears" By Les Seago MSU Research Reporter

Fear of fat fans the tobacco flame among many smokers who would like to quit, according to an MSU psychologist. Their fears may be justified, he says.

Also featured, "Saving the Blues" By Laurie Finn

Dr. David Evans researches the roots of regional music of the South. Dr. Steven Ross examines computer programs and the adaptation of mathematics instruction to microcomputers. Excellence is what their work has in common.

University Of Memphis Magazine, 2015 Fall

University of Memphis Magazine

Cover Story, "Making the Grades " by Greg Russell

From a near-zero graduation rate 15 years ago in men’s basketball to one of the best student-athlete success rates around, Tiger players score both on and off the playing surface.

Also featured: The Columns Alumni Review

University Of Memphis Magazine, 2020 Fall

University of Memphis Magazine

In this issue, "I Want To Go Where I Can Help" By David Waters, UofM Institute for Public Service Reporting, Illustration by Catherine Knowles

UofM alum Janice Ballard joined a group of health professionals helping fight COVID-19 in the Navajo Nation, one of the hardest hit areas in the world per capita.

Also featured, "Unearthing Ancient Egypt" by Trent Shadid

As an expert on ancient Egypt, the work of UofM history professor Suzanne Onstine is providing insight into the lives of everyday Egyptians.

Re-Assessing The Influence Of Particle-Hosted Sulphide Precipitation On The Marine Cadmium Cycle, Gregory F. De Souza, Derek Vance, Matthias Sieber, Tim M. Conway, Susan H. Little

Marine Science Faculty Publications

It has been inferred that the marine distributions of the micronutrient cadmium (Cd) and its stable isotope composition (expressed as δ¹¹⁴Cd) bear widespread and unambiguous evidence for loss of Cd from the shallow water column through the formation of particle-associated cadmium sulphide (CdS) in oxygen minimum zones (OMZs). In this review, we bring together elemental and isotopic datasets from the dissolved and particulate Cd pools in order to unravel the multiple, overlapping controls on the distribution of Cd and δ¹¹⁴Cd, and demonstrate that the global dataset challenges this view. By far the most important control on …

Investigation Of Nuclear Envelope-Associated Progeria Proteins During Nuclear Envelope Rupture, Rhiannon Marie Sears

Dissertations and Theses

The nuclear envelope (NE) is a specialized extension of the endoplasmic reticulum critical for the proper protection and organization of the genome. This interphase-only structure is host to several proteins and their interacting constituents that have various and diverse cellular roles. Mutations in NE constituents are associated with several human diseases. A subset of these diseases, known as progeria syndromes, are characterized by the premature presentation of physiological aging in a variety of tissues. Mutations in the genes LMNA and BANF1, which encode the A-type lamins and barrier-to-autointegration factor (BAF), can lead to these multisystem progeroid diseases. Though the underlying …

Exosomes Derived From Metastatic Colorectal Cancer Mediate Perineural Invasion: Implications For Therapeutic Intervention, Cole Knoblich

Dissertations and Theses

Colorectal cancer (CRC) is one of the most globally diagnosed cancers and a leading cause of cancer-related mortality. Metastatic CRC (mCRC) significantly impacts patient outcome and diminishes overall survival outcomes. Despite advances in therapeutic treatment, the 5-year survival rate for mCRC is < 20% and several patients fail to have a beneficial response to available treatments. This could be attributed to alternative routes of mCRC in which current treatments are unable to block these routes of cancer spread. One such alternative route involves the spread of cancer along the nerve termed perineural invasion (PNI) which is an independent prognostic factor in CRC. The tumor microenvironment (TME) is comprised of several different cell types that have been investigated for their role in cancer pathogenesis. Several studies have shown that cells of the TME can provide a supportive environment that allows for the growth and spread of cancer. Primary focus has been placed on cancer, stromal, and immune cells in the TME, but the role of neuronal cells in cancer pathogenesis is still unknown. Since cells of the TME can communicate through the secretion of exosomes, cancer-derived exosomes have gained widespread interest for their role in cancer pathogenesis. This study investigated how CRC-derived exosomes prime neuronal cells and contribute to PNI. By utilizing transwell co-culture systems and conditioned media experiments, we were able to determine that CRC cells secrete extracellular vesicles (EVs) that induce neuronal cell differentiation and growth, and inhibit apoptosis. Furthermore, utilization of an exosome inhibitor revealed that blocking exosome production in CRC cells could attenuate these affects. CRC- derived exosomes were collected utilizing a modified-iodixanol gradient technique and further characterization revealed an enriched pool of exosomes in fractions. Furthermore, proteomic analysis showed several proteins contained in CRC-derived exosomes that were involved in neuronal cell differentiation and growth. Lastly, treatment of CRC cells with the pharmacological tool Veratridine (VTD) was able to attenuate neuronal cell differentiation and growth and alter the proteomic profiles found in CRC-derived exosomes. In addition, VTD treatment slowed the spread of CRC along the sciatic nerve of an NIH-III nude mouse model of PNI.

An Appetite For Crime: Case Studies Of Cannibalism And The Criminological Theories That Explain It, Eponine Romo

Undergraduate Honors Theses

One of the most important aspects of studying crime is identifying how and why certain crimes happen. There are several questions one should ask: Why did this event happen? What caused this to happen? How could it have been stopped? Criminologists use various theories to seek the answer to these questions regarding diverse types of crimes from petty crimes, such as stealing a pack of gum to major, violent crimes, such as cannibalism, the latter of which will be analyzed here. The goal is to prevent these crimes from happening in the future by identifying why they are happening now. …

Quigley Hall, Central Washington University

What is in a Name?

No abstract provided.

Dean Hall, Central Washington University

What is in a Name?

No abstract provided.