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Lysoptosis Is An Evolutionarily Conserved Cell Death Pathway Moderated By Intracellular Serpins, Cliff J Luke, Stephanie Markovina, Misty Good, Ira E Wight, Brian J Thomas, John M Linneman, Wyatt E Lanik, Olga Koroleva, Maggie R Coffman, Mark T Miedel, Qingqing Gong, Arlise Andress, Marlene Campos Guerrero, Songyan Wang, Liyun Chen, Wandy L Beatty, Kelsey N Hausmann, Frances V White, James A J Fitzpatrick, Anthony Orvedahl, Stephen C Pak, Gary A Silverman

Open Access Publications

Lysosomal membrane permeabilization (LMP) and cathepsin release typifies lysosome-dependent cell death (LDCD). However, LMP occurs in most regulated cell death programs suggesting LDCD is not an independent cell death pathway, but is conscripted to facilitate the final cellular demise by other cell death routines. Previously, we demonstrated that Caenorhabditis elegans (C. elegans) null for a cysteine protease inhibitor, srp-6, undergo a specific LDCD pathway characterized by LMP and cathepsin-dependent cytoplasmic proteolysis. We designated this cell death routine, lysoptosis, to distinguish it from other pathways employing LMP. In this study, mouse and human epithelial cells lacking srp-6 homologues, mSerpinb3a and SERPINB3, …

Antibiotic Exposure And Acquisition Of Antibiotic-Resistant Gram-Negative Bacteria Among Outpatients At A Us Veterans Affairs Medical Center, Brigid M. Wilson, Sunah Song, Federico Perez, Robin L.P. Jump

Faculty Scholarship

Objectives: To assess the prevalence of antibiotic-resistant gram-negative bacteria (R-GNB) among patients without recent hospitalization and to examine the influence of outpatient antibiotic exposure on the risk of acquiring R-GNB in this population. Design: 2-year retrospective cohort study. Setting: Regional Veterans Affairs healthcare system. Patients: Outpatients at 13 community-based clinics. Methods: We examined the rate of acquisition of R-GNB within 90 days following an outpatient visit from 2018 to 2019. We used clinical and administrative databases to determine and summarize prescriptions for systemic antibiotics, associated infectious diagnoses, and subsequent R-GNB acquisition among patients without recent hospitalizations. We also calculated the …

Graphene Oxide Functionalized Biosensor For Detection Of Stress-Related Biomarkers, Erican Santiago, Shailu Shree Poudyal, Sung Y. Shin, Hyeun Joong Yoon

Michigan Tech Publications, Part 1

A graphene oxide (GO)-based cortisol biosensor was developed to accurately detect corti-sol concentrations from sweat samples at point-of-care (POC) sites. A reference electrode, counter electrode, and working electrode make up the biosensor, and the working electrode was functional-ized using multiple layers consisting of GO and antibodies, including Protein A, IgG, and anti-Cab. Sweat samples contact the anti-Cab antibodies to transport electrons to the electrode, resulting in an electrochemical current response. The sensor was tested at each additional functionalization layer and at cortisol concentrations between 0.1 and 150 ng/mL to determine how the current response differed. A potentiostat galvanostat device was …

Butte Priority Soils Operable Unit (Bpsou) Draft Final Insufficiently Reclaimed Sites - Field Sampling Plan (Fsp) Bres No. 30 – Atlantic-1, Mike Mcanulty

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Founders' Day Convocation (2022 Program, Video And Slides), Illinois Wesleyan University

Founders’ Day

No abstract provided.

Optimizing Patient Selection For Irreversible Electroporation Of Locally Advanced Pancreatic Cancer: Analyses Of Survival, Matthew R. Woeste, Khaleel D. Wilson, Edward J. Kruse, Matthew J. Weiss, John D. Christein, Rebekah R. White, Robert C. G. Martin Ii

School of Medicine Publications

Background: Irreversible electroporation (IRE) has emerged as a viable consolidative therapy after induction chemotherapy, in which this combination has improved overall survival of locally advanced pancreatic cancer (LAPC). Optimal timing and patient selection for irreversible electroporation remains a clinically unmet need. The aim of this study was to investigate preoperative factors that may assist in predicting progression-free and overall survival following IRE.

Methods: A multi-institutional, prospectively maintained database was reviewed for patients with LAPC treated with induction chemotherapy followed by open-technique irreversible electroporation from 7/2015-5/2019. RECIST 1.1 criteria were used to assess tumor response and radiological progression. Overall survival (OS) …

Fbxw7-Mediated Erk3 Degradation Regulates The Proliferation Of Lung Cancer Cells, Hyun-Jung An, Cheol-Jung Lee, Ga-Eun Lee, Youngwon Choi, Dohyun Jeung, Weidong Chen, Hye Suk Lee, Han Chang Kang, Joo Young Lee, Dae Joon Kim

School of Medicine Publications

Extracellular signal-regulated kinase 3 (ERK3) is an atypical member of the mitogen-activated protein kinase (MAPK) family, members of which play essential roles in diverse cellular processes during carcinogenesis, including cell proliferation, differentiation, migration, and invasion. Unlike other MAPKs, ERK3 is an unstable protein with a short half-life. Although deubiquitination of ERK3 has been suggested to regulate the activity, its ubiquitination has not been described in the literature. Here, we report that FBXW7 (F-box and WD repeat domain-containing 7) acts as a ubiquitination E3 ligase for ERK3. Mammalian two-hybrid assay and immunoprecipitation results demonstrated that ERK3 is a novel binding partner …

Grasslands Classification In Yobe State Nigeria Using Integrated Orderly Classification System Of Grassland (Iocsg), S. Usman, M. G. Bala, Y. Shen

IGC Proceedings (1977-2023)

Integrated Orderly Classification System of Grassland (IOCSG) was initiated by Professor Ji-Zhou Ren in the 1960s and it has satisfactorily served to direct theory and practice of grassland classification for the last several decades in China. In this study IOCSG is adopted to classify the grasslands in Yobe state Nigeria, using 30 years (1984-2013) data on precipitation and temperature from 12 satellite stations across the state. Humidity (moisture index) was estimated as the ratio of precipitation per annum to annual temperature. The results revealed a trend of decline in precipitation and moisture index with latitude; from 772.63 mm and 0.72 …

Forb Counts In Grassland Are Sensitive To Analytical Method, Gene A. Fults

IGC Proceedings (1977-2023)

An analysis of forbs from 9 years of Plant Census protocol (NRI 2019) is compared to data from the Line Point Intercept (LPI) protocol. Both protocols were conducted on the same geospatially separate macroplots (N=1230). The macroplots locations were stratified by NRI based on a land hierarchy which has had long-term use in the United States (Salley etal 2015). This ties vegetation and land together in the data. The analysis showed that the 15-minute plant census method increased forb species data by 29 percent over the LPI method which takes 40-60 minutes. Both methods captured similar total ratios and rank …

The Relationship Between Grassland Composition On Stemborer Abundance On Grasses Surrounding Maize Farms, Nelima Maryselah, Mutyambai M. Daniel, Kuyah Shem, Netondo Godfrey, Mattias Jonsons

IGC Proceedings (1977-2023)

Grasslands are known to host crop pests and may act as the reservoir of pests affecting maize production. This research aimed at determining the effect of grassland composition on abundance of stemborers on grasses sorrouding maize farms in western Kenya. The experimental set up followed a complete random block design (CRBD); with four elevations at Lambwe, Homabay, Luanda and Mt. Elgon assigned as blocks based on different altitudes. Sampling was done in two seasons, during the short rain season of 2019 and the long rain season of 2020. Grasslands surrounding four pairs of push-pull and non-push-pull maize farms were assessed. …

Describing Cattle Grazing Distribution In Communal Rangelands Of The Eastern Cape South Africa: Towards Improving Livestock Rangeland Use, B. Gusha, A. R. Palmer

IGC Proceedings (1977-2023)

This study used global positioning systems (GPS) to understand grazing distribution of three cattle in the communal rangeland of Eastern Cape, South Africa. Free ranging cattle were randomly selected from participating households and were fitted with neck GPS collars, which recorded geographic position every five-minute interval during wet and dry season. The study identified three production domains where cattle were reported to be spending most of their time grazing, separated by bound polygons on google earth Pro to further extract MODIS EVI???. The recorded GPS points were loaded into google earth Pro to analyse the distribution of cattle grazing in …

Degradation Issue Of Fescue-Forbs Rangeland In The Mountain Steppe Of Mongolia, Otgontuya Lkhagvajav, Lkhagvajav Namdag, Munkhbat Chimed

IGC Proceedings (1977-2023)

The total area of Fescue-forbs rangeland in Mongolia is 10 million hectares, accounting for 8.5% of total rangeland area. The Fescue-forbs rangeland is the main type of rangeland in the mountain steppe. The Mongolian rangeland has degraded in the last two decades mainly due to overgrazing and climate change. The main objective of this study was to determine the change of plant community and reduction of frequency and productivity of the dominant species Festuca lenensis in different degradation states of rangeland. The dominant species and main plant functional groups have completely changed in Fescue-forbs rangeland in all three states of …

[Re/De]Generation Colloquy Volume 1, Abby Stocker, Leah Patton, Brad Cox, Jacob Manning, Roberta Fultz, Jared Hedges, Stacie Lewis

Colloquy Undergraduate Research Journal

Contents

Editor’s Statement 5

Communication Studies

On Ideals in Romantic Relationships 25

Paul Hjellming, Lori Bergstrom, Bo Johnson, and Eric Osmondson

Perception of Profanity in Interpersonal Relationships 47

Ashley Pivaronas, Jessica Benham, and Stephanie Melhaff

The Genre of the Meme 67

Thomas Monson

Mathematics

Cooking Up the Optimal Baking Algorithm 7

Tony Burand, Michael Tetzlaff, and Jacob Smith

Literary Criticism

“What to Sight and Smell Was Sweet”: Flowers and Gardening in Paradise Lost 41

Linnea White

The Case of “Brown” 59

Sara Ellingsworth

Biblical and Theological Studies

The Markan Narrative of the Hemorrhaging Woman: Injustice Through Systems Then and Now …

Observations On Regeneration Of The Pedipalp And Legs Of Scorpions, Martin Watz, Jason A. Dunlop

Euscorpius

An Opisthacanthus asper (Peters, 1861) (Hormuridae) shows a relatively rare example of pedipalp regeneration in which the lost tibia and tarsus was replaced by a smaller, curved element of uncertain homology to either the fixed or free finger. A comparable abnormal palp described in the literature hints that pedipalps can only regenerate a structure of this form, regardless of the site of amputation. An Olivierus caucasicus (Nordmann, 1840) (Buthidae) is described in which claws (pretarsus) of leg III regenerated directly at the distal end of the tibia, while in leg IV the claws regenerated at the end of a truncated …

Inducing Cognitive Reflection And Its Impact On Contradictory Belief Holding, Meg Powers, Marci Decaro

Posters-at-the-Capitol

The idea that there are 2 distinct processing modes is seen throughout social and cognitive psychology research. One mode is generally fast, automatic, and relatively effortless, while the other is slow, systematic, and effortful. One mechanism of effortful processing is cognitive reflection which is one’s ability to reflect on their intuition. The Cognitive Reflection Test (CRT) is a 3-item measure that quantifies a person’s ability to recognize an intuitive answer as incorrect and identify the correct response. Additionally, a yea-yeaing score was collected to measure how often a person agrees with a statement and its opposite (i.e., endorses contradictory beliefs). …

One-Pot Conversion Of Carboxylic Acids To Aldehydes, Hannah Khan, Michael Nantz, Saurin Sutaria

Posters-at-the-Capitol

There are many different ways to convert a carboxylic text acid to an aldehyde. A common way to accomplish this transformation is to use multiple steps (e.g., reduction of the carboxylic acid to the corresponding alcohol followed by selective oxidation to the aldehyde). Our success in reducing the diacid muconic acid (1) to muconaldehyde (4) in a relatively mild, one-pot process has led us to examine whether the method can be expanded to other carboxylic acids. We report here our findings using benzoic acid as a representative substrate.

Breadth Of Vaccinated Cancer Patient Humoral Response To Sars-Cov-2 Spike Protein And Rbd Variants, Julia Corman, Joey Breckenridge Jr., Adrienne M. Bushau-Sprinkle, Krystal T. Hamorsky, Joshua L. Fuqua

Posters-at-the-Capitol

SARS-CoV-2, the virus responsible for the COVID-19 of which several variants have emerged, such as the B.1.351 SARS-CoV-2 variant. The Receptor Binding Domain (RBD), located within the Spike protein is an immunogenic epitope for potent neutralizing antibodies. Current mRNA vaccines encode for the Spike protein, allowing the body to build antigen-specific antibodies. Assays measuring protective antibodies are essential to manage the COVID-19 pandemic and can be used as a platform for variant screening. RBD-foldon 2.2 is a novel antigen produced by fusing RBD with the trimerization domain Fibritin from Bacteriophage T4. Its amino acid sequence is based on the original …

Application Of Technology Resources To Library Information Processing In Academic Libraries In Nigeria, Peter Olubunmi Olubiyo

Library Philosophy and Practice (e-journal)

The paper is about Application of Technology Resources to Library Information Processing in Academic Libraries in Nigeria. The electronic age has made users search beyond print media while looking for information resources. Latest research work are sent across the world through electronic means and no serious researcher of this age ever depends solely on print media rather the use of internet, websites, e-mail etc are the language of the day. Functionally, university libraries of the current dispensation provide access to both print and electronic resources to serve users and to increase the visibility of their institutions, and as a measure …

Decomposition Rates Of Organic Material Across Herbivore Treatments In A Nutrient-Rich Semi-Arid Sodic Savanna, L. L. Erasmus, H. Van Coller, S. Claassens, F. Siebert

IGC Proceedings (1977-2023)

Decomposition is a major determinant of terrestrial nutrient cycling and therefore an important regulator of ecosystem structure and function. It has been widely documented that large mammalian herbivores (LMH) act as a significant driver of changes to aboveground structure and modifications to edaphic properties. Little is known about the role of herbivory, and particularly the loss thereof, in mediating essential ecological processes in a herbivore-adapted system. The Nkuhlu exclosures, a large-scale, long-term exclusion experiment in the Kruger National Park, South Africa, provided an opportunity to explore the effects of herbivory and/or its long-term exclusion on decomposition and stabilisation of detrital …

Comparative Anatomical Study Of The Grasses In The Range Lands Of Kovilpatti, Tamil Nadu Of India, B. Makesh Kumar, J. Stephan, P. Kumar

IGC Proceedings (1977-2023)

Grasses are the crucial component of the rural rangeland ecology. Besides the morphological studies, micro-morphological characters such as stem anatomy were also investigated in this study. So as to get detailed understanding about variations during the summer and monsoon within species and also differentiating the species with the help of supplementary characters such as number of cortical layers and vascular bundles, xylem vessel length etc. In grass anatomy the characters are rather constant for some species and highly variable for others probably often due to the climatic variation. Still the characters are very helpful to differentiate and determine the genus. …

Smokejumper Obituary: Siemens, Roger M. (Missoula 1959), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Targeting The Fusion Process Of Sars-Cov-2 Infection By Small Molecule Inhibitors, Seung Bum Park, Parker Irvin, Zongyi Hu, Mohsin Khan, Xin Hu, Qiru Zeng, Catherine Chen, Miao Xu, Madeleine Leek, Ruochen Zang, James Brett Case, Wei Zheng, Siyuan Ding, T. Jake Liang

Open Access Publications

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a serious threat to global public health, underscoring the urgency of developing effective therapies. Therapeutics and, more specifically, direct-acting antiviral development are still very much in their infancy. Here, we report that two hepatitis C virus (HCV) fusion inhibitors identified in our previous study, dichlorcyclizine and fluoxazolevir, broadly block human coronavirus entry into various cell types. Both compounds were effective against various human-pathogenic CoVs in multiple assays based on vesicular stomatitis virus (VSV) pseudotyped with the spike protein and spike-mediated syncytium formation. The antiviral effects …

Hiv-1 Genetic Diversity A Challenge For Aids Vaccine Development: A Retrospective Bibliometric Analysis, Abdur Rashid, Kang Li, Yi Feng, Tauseef Ahmad, Yimam Getaneh, Yueyang Yu, Xiaoyan Hu, Syed Hani Abidi, Yiming Shao

Department of Biological & Biomedical Sciences

Background: Despite recent advances in human immunodeficiency virus-1 (HIV-1) prevention, a fast, safe, and effective vaccine will probably be necessary to end the HIV/AIDS pandemic. This study was conducted to evaluate global research trends and map the key bibliometric indices in HIV-1 genetic diversity from 1998 to 2021.
Methods: A comprehensive online search was conducted in the Web of Science Core Collection database to retrieve published literature on HIV-1 genetic diversity. Key bibliometric indicators were calculated and evaluated using HistCiteTM, Bibliometrix: An R-tool, and VOSviewer software for windows.
Results: A total of 2,060 documents written by 9,201 authors and published …

Role Of International Research Experiences In The Development Of Women Of Color In Chemistry, R D. Davis, L Winfield, D Spivak, Z S. Wilson-Kennedy, R D. Davis

Faculty Publications

As undergraduate students cultivate their scientific knowledge and abilities, several high-impact educational practices such as undergraduate research and global experiences have proven exponentially beneficial for their development and success. Current literature on underrepresented racial and ethnic minorities has focused primarily on the impact of undergraduate research on this subpopulation. However, fewer studies have explored the impact of global research experiences and women of color in international research experiences. Grounded in the theoretical frameworks of Social Cognitive Career Theory, Intersectionality, and Community Cultural Wealth, this present study explored the impact of international undergraduate research experiences on women of color from groups …

Current Methods And Future Directions In Avian Diet Analysis, Brandon D. Hoenig, Allison M. Snider, Anna M. Forsman, Keith A. Hobson, Steven C. Latta, Eliot T. Miller, Michael J. Polito, Luke L. Powell, Samantha L. Rogers, Thomas W. Sherry, David P.L. Toews, Andreanna J. Welch, Sabrina S. Taylor, Brady A. Porter

Faculty Publications

Identifying the composition of avian diets is a critical step in characterizing the roles of birds within ecosystems. However, because birds are a diverse taxonomic group with equally diverse dietary habits, gaining an accurate and thorough understanding of avian diet can be difficult. In addition to overcoming the inherent difficulties of studying birds, the field is advancing rapidly, and researchers are challenged with a myriad of methods to study avian diet, a task that has only become more difficult with the introduction of laboratory techniques to dietary studies. Because methodology drives inference, it is important that researchers are aware of …

A Call For The Library Community To Deploy Best Practices Toward A Database For Biocultural Knowledge Relating To Climate Change, Martha B. Lerski

Publications and Research

Abstract

Purpose – In this paper, a call to the library and information science community to support documentation and conservation of cultural and biocultural heritage has been presented.

Design/methodology/approach – Based in existing Literature, this proposal is generative and descriptive— rather than prescriptive—regarding precisely how libraries should collaborate to employ technical and ethical best practices to provide access to vital data, research and cultural narratives relating to climate.

Findings – COVID-19 and climate destruction signal urgent global challenges. Library best practices are positioned to respond to climate change. Literature indicates how libraries preserve, share and cross-link cultural and scientific knowledge. …

Identifying Conifer Tree Vs. Deciduous Shrub And Tree Regeneration Trajectories In A Space-For-Time Boreal Peatland Fire Chronosequence Using Multispectral Lidar, Humaira Enayetullah, Laura Chasmer, Christopher Hopkinson, Dan Thompson, Danielle Cobbaert

Aspen Bibliography

Wildland fires and anthropogenic disturbances can cause changes in vegetation species composition and structure in boreal peatlands. These could potentially alter regeneration trajectories following severe fire or through cumulative impacts of climate-mediated drying, fire, and/or anthropogenic disturbance. We used lidar-derived point cloud metrics, and site-specific locational attributes to assess trajectories of post-disturbance vegetation regeneration in boreal peatlands south of Fort McMurray, Alberta, Canada using a space-for-time-chronosequence. The objectives were to (a) develop methods to identify conifer trees vs. deciduous shrubs and trees using multi-spectral lidar data, (b) quantify the proportional coverage of shrubs and trees to determine environmental conditions driving …

Associations Between Feeding Behaviors Collected From An Automated Milk Feeder And Neonatal Calf Diarrhea In Group Housed Dairy Calves: A Case-Control Study, Meridith H. Conboy, Charlotte B. Winder, Melissa C. Cantor, Joao H. C. Costa, Michael A. Steele, Catalina Medrano-Galarza, Taika E. Von Konigslow, Amanda Kerr, Dave L. Renaud

Animal and Food Sciences Faculty Publications

The objective of this case-control study was to determine if feeding behavior data collected from an automated milk feeder (AMF) could be used to predict neonatal calf diarrhea (NCD) in the days surrounding diagnosis in pre-weaned group housed dairy calves. Data were collected from two research farms in Ontario between 2017 and 2020 where calves fed using an AMF were health scored daily and feeding behavior data (milk intake (mL/d), drinking speed (mL/min), number of rewarded or unrewarded visits) was collected. Calves with NCD were pair matched to healthy controls (31 pairs) by farm, gender, and age at case diagnosis …

Heterologous Production Of Plant Secondary Metabolites Resveratrol And Caffeic Acid In E.Coli And Synechocystis Sp. Pcc 6803, Yong Xue

Theses and Dissertations

Caffeic acid and resveratrol are polyphenol compounds produced by plants as secondary metabolites to protect against biotic and abiotic stresses. In the last decades, numerous studies have demonstrated the beneficial effects of these compounds on human health as anticancer, antioxidant, anti-virus and anti-inflammatory agents. In plants, both caffeic acid and resveratrol are synthesized either from phenylalanine or tyrosine. Recently, heterologous production of phenylpropanoids using genetically engineered bacteria is gaining more and more attentions from industry because it can dramatically improve the yield with reduced costs. The major aim of the work is to explore the possibility of using Synechocystis or …

Massively Parallel Assessment Of The Functional Effects Of De Novo Autism Spectrum Disorder Patient-Derived Genetic Variants In 3’ Untranslated Regions, Tomas Lagunas Jr

Arts & Sciences Graduate Student Theses and Dissertations

Human genetic studies have identified a large number of patient-derived de novo variants in presumptive regulatory regions of the genome that pose a challenge for interpretation of their effects since the impact of regulatory variants is highly dependent on the cellular context. In particular, current investigations have made significant investments in sequencing the genomes of autism spectrum disorder (ASD) families as a diagnostic method and to further understand the genetic architecture of this complex neurodevelopmental disease. These studies have revealed an enrichment of mutations in the untranslated regions (UTRs) of genes, which are noncoding regulatory regions, in ASD genomes. Assessment …