Digital Commons Network^™

Establishment Of A Melanoma Esc-Gemm Platform And Its Use To Study Pten Tumor Suppressor Functions, Ilah Bok

USF Tampa Graduate Theses and Dissertations

The cumbersome and time-consuming process of generating new mouse strains and multiallelic experimental animals often hinders the use of genetically engineered mouse models (GEMM) in cancer research. In chapter 2, we describe the development and validation of an embryonic stem cell (ESC)-GEMM platform for rapid modeling of melanoma in mice. The platform incorporates 12 clinically relevant genotypes composed of combinations of four driver alleles (LSL-BrafV600E, LSL-NrasQ61R, PtenFlox, and Cdkn2aFlox) and regulatory alleles to spatiotemporally control the perturbation of genes of interest. The ESCs produce high-contribution chimeras, which recapitulate the melanoma phenotypes of conventionally bred mice. Using the ESC-GEMM platform to …

Defining And Modeling Pericyte-Induced Capillary Network Assembly, Capillary Regression Preceding Pericyte-Stimulated Fibrosis, And Lymphatic Capillary Regression, Scott Stephen Kemp

USF Tampa Graduate Theses and Dissertations

Capillary formation and maturation can only occur through a series of highly orchestrated events that requires the coordinated efforts of both endothelial cells (ECs) and pericytes. While ECs are perfectly capable of creating tube networks on their own, the maturation and stabilization of these tube networks require the presence of a second cell type, pericytes, to create stable capillary networks. Previous studies have identified factors required for pericyte recruitment to EC tube networks, however, we hypothesized that there were still unaccounted for EC-derived molecules that were contributing to pericyte recruitment. In the first part of this study, we used serum-free, …

Targeting The Side-Chain Convergence Of Α-Helical Hot Spots To Design Small-Molecule Mimetics Disrupting Protein-Protein Interaction, Zhen Wang

USF Tampa Graduate Theses and Dissertations

α-Helix accounts for the largest class of protein secondary structures and plays a key role in mediating protein-protein interactions (PPIs). Small-molecule α-helix mimetics mimic the structural features of α-helical hot spots and offer a powerful tool to unravel complex signaling networks and mitigate aberrant PPIs. In this dissertation, the side-chain conformational convergence of α-helical hot spots was disclosed, and insights into the side-chain convergence were exploited to instruct the design of small-molecule α-helix mimetics. The first chapter of this dissertation provides an overview of the characteristics of α-helical hot spots and current strategies to design small-molecule α-helix mimetics. In chapter …

Re-Centering And De-Centering ‘Race’: An Analysis Of Direct-To-Consumer Genetic Testing Organizational Websites, Beatriz Nieto-Fernandez

USF Tampa Graduate Theses and Dissertations

As direct-to-consumer (DTC) genetic testing technologies become an ever-expanding business in the United States, this dissertation project investigates the ways in which two leading organizations, AncestryDNA and 23andMe, utilize discourses of ‘race’ to connect with their customers. This study is particularly significant because it opens up new avenues of research in the field of organizational communication by engaging with a new genre of organizations and the complexities of biocapitalism, in which genetic data is commodified for consumption. Blending computational scraping of data and manual qualitative analysis of organizational texts and videos available on the corporate websites, while using postcolonial studies …

Synthesis Of Thiol-Acrylate Hydrogels For 3d Cell Culture And Microfluidic Applications, Anowar Hossain Khan

LSU Doctoral Dissertations

Globally cell culture is an $18.98 billion industry as of 2020, with an 11.6 percent annual growth rate. Drug discovery has an estimated worth of $69.8 billion in 2020 and is predicted to grow to $110.4 billion by 2025. Three-dimensional (3D) cell culture of cancer cells is one of the rapidly growing felids since it better recapitulates in vivo conditions compared to two-dimensional (2D) models. However, it is challenging to grow 3D tumor spheroids outside the body, and some of the existing technology can generate these spheroids outside the human body but poorly mimic in vivo tumor models. Therefore, there …

An Investigation Of A Novel Broad Autism Phenotype: Increased Facial Masculinity Among Parents Of Children On The Autism Spectrum, Diana Weiting Tan, Syed Zulqarnain Gilani, Gail A. Alvares, Ajmal Mian, Andrew J. O. Whitehouse, Murray T. Maybery

Research outputs 2022 to 2026

The broad autism phenotype commonly refers to sub-clinical levels of autistic-like behaviour and cognition presented in biological relatives of autistic people. In a recent study, we reported findings suggesting that the broad autism phenotype may also be expressed in facial morphology, specifically increased facial masculinity. Increased facial masculinity has been reported among autistic children, as well as their non-autistic siblings. The present study builds on our previous findings by investigating the presence of increased facial masculinity among non-autistic parents of autistic children. Using a previously established method, a 'facial masculinity score' and several facial distances were calculated for each three-dimensional …

Cistrome And Transcriptome Analysis Identifies Unique Androgen Receptor (Ar) And Ar-V7 Splice Variant Chromatin Binding And Transcriptional Activities, Paul Basil, Matthew J Robertson, William E Bingman, Amit K Dash, William C Krause, Ayesha A Shafi, Badrajee Piyarathna, Cristian Coarfa, Nancy L Weigel

Faculty, Staff and Students Publications

The constitutively active androgen receptor (AR) splice variant, AR-V7, plays an important role in resistance to androgen deprivation therapy in castration resistant prostate cancer (CRPC). Studies seeking to determine whether AR-V7 is a partial mimic of the AR, or also has unique activities, and whether the AR-V7 cistrome contains unique binding sites have yielded conflicting results. One limitation in many studies has been the low level of AR variant compared to AR. Here, LNCaP and VCaP cell lines in which AR-V7 expression can be induced to match the level of AR, were used to compare the activities of AR and …

Geotemporospatial And Causal Inferential Epidemiological Overview And Survey Of Usa Cannabis, Cannabidiol And Cannabinoid Genotoxicity Expressed In Cancer Incidence 2003–2017: Part 2 – Categorical Bivariate Analysis And Attributable Fractions, Albert Stuart Reece, Gary Kenneth Hulse

Research outputs 2022 to 2026

Background: As the cannabis-cancer relationship remains an important open question epidemiological investigation is warranted to calculate key metrics including Rate Ratios (RR), Attributable Fractions in the Exposed (AFE) and Population Attributable Risks (PAR) to directly compare the implicated case burden between emerging cannabinoids and the established carcinogen tobacco. Methods: SEER*Stat software from Centres for Disease Control was used to access age-standardized state census incidence of 28 cancer types (including “All (non-skin) Cancer”) from National Cancer Institute in US states 2001–2017. Drug exposures taken from the National Survey of Drug Use and Health 2003–2017, response rate 74.1%. Federal seizure data provided …

Laws Governing Manslaughter By Food Safety Crimes In The United Kingdom, Australia, Bangladesh And India: A Critical Review, S M. Solaiman

North Carolina Journal of International Law

No abstract provided.

Alzheimer's And Patient Caregiver Burnout: A Review Of The Literature, Madeline Hekeler

James Madison Undergraduate Research Journal (JMURJ)

The term “silent epidemic” is fitting for Alzheimer’s disease (AD), as its negative impact is widely felt but rarely discussed. Burnout among AD caregivers has become an epidemic of its own as caregivers experience an increase in health risks, stress, and financial burden. This literature review focuses on caregiver burnout and how imperative it is that caregivers are better supported in their role. Researchers have developed instruments to assess and intervene in caregiver burnout that have shown effectiveness among caregivers and their families.Nevertheless, further longitudinal research is warranted regarding more effective interventions, including stress management and social support mechanisms.

Assessment Of The Influence Of Socio-Demographic And Personality Factors On Health Behaviours In Healthy Postmenopausal Women, Daria Schneider-Matyka, Elzbieta Grochans, Agnieszka Samochowiec, Malgorzata Szkup, Katarzyna Augustyniuk, Anna Jurczak, Malgorzata Sterczak

Baltic Journal of Health and Physical Activity

Background: Lifestyle and health behaviours are determined by the level of knowledge, beliefs, existing dysfunctions, and interpersonal relationships, as well as personality factors. The aim of this study was to assess health behaviours undertaken by postmenopausal women, and to analyze how they are influenced by personality factors. Material/Method: The study included 196 postmenopausal women at the mean age of 56.80 +/- 4.08. The Health Behaviour Inventory and a personality questionnaire (NEO-FFI) were used. Results: In the study, women living in cities of over 100.000 residents scored higher than those living in smaller towns (p<0.05). The level of neuroticism negatively correlated with overall health behaviours, health practices, and a positive mental attitude (p<0.05). The level of conscientiousness negatively correlated with health behaviours, a positive mental attitude, and preventive behaviours (p<0.05). Positive correlations were observed between the level of extraversion and overall health behaviours, preventive behaviours, normal eating habits, health practices, and a positive mental attitude (p<0.05). Conclusions: 1. Raising the awareness through educational programmes implemented as part of care provided by primary care centres (especially in smaller towns and rural areas) could improve the level of healthful behaviours undertaken by postmenopausal women. 2. Health behaviours are also greatly dependent on personality traits, which are biologically determined and relatively stable.

Resiliency In Mainstream School Teachers Versus Special School Teachers, Aleksandra Sadziak, Wojciech Wilinski, Marta Wieczorek

Baltic Journal of Health and Physical Activity

Background: The aim of this study was to identify and evaluate significant differences in the resiliency level in investigated mainstream school teachers and special school teachers with particular regard to physical education teachers. Material/Methods: The study was conducted among 198 teachers from Lower Silesia and Silesia provinces, including 26 physical education teachers from special schools, 62 teachers of other subjects from special schools, 44 physical education teachers from mainstream schools and 65 teachers of other subjects from mainstream schools. A diagnostic survey was the research method, a survey was the technique, and the Resiliency Questionnaire in a Polish adaptation of …

The Effect Of Regular Aerobic Exercises On Premenstrual Syndrome In Sedentary Women, Guner Cicek

Baltic Journal of Health and Physical Activity

Background: Premenstrual symptoms (PMS) occur very commonly and cause a negative impact on the overall quality of women’s lives. The aim of this study was to assess the PMS symptoms between women who exercise and non-exercising sedentary women. Material/Methods: A total of 220 participants were studied. N=100 exercising group and N=120 non-exercising were selected as participants. The exercising group was selected from women who regularly do aerobic exercise (walk-running) for 3-5 days a week, within a 60 minute time period for each exercise session for 4 months. Results: The mean number of menstruations in a year and the frequency of …

The Effect Of N‪Icotine On Oral Health, Grzegorz Trybek, Magda Aniko-Wlodarczyk, Olga Preuss, Piotr Kuligowski, Ewa Gabrysz-Trybek, Aleksandra Suchanecka, Patryk Niewczas, Anna Grzywacz

Baltic Journal of Health and Physical Activity

Background: Nicotine absorbed by the skin and also by the digestive, respiratory and excretory systems is one of the most known toxins found in tobacco. ‪This work reviews available literature on the influence of nicotine on human health, and in particular on the oral cavity. Material/Methods: ‪A review of literature related to the topic of the paper. Results: Numerous studies indicate that nicotine adversely affects human health. ‪It exerts a negative effect at the cellular level by modifying metabolism, and disturbs the interactions between tissues, which strongly promotes the formation of cancer-like changes. Nicotine causes numerous pathologies in the respiratory, …

Fracture Of The Lateral Tibial Condyle With Depression Of The Articular Surface - A Case Report, Krzysztof Ficek, Paulina Cyganik, Magdalena Strozik, Anna Serniak, Joanna Rajca

Baltic Journal of Health and Physical Activity

Background: Fractures of the proximal epiphysis of the tibia constitute one of the more frequently occurring types of sports injuries, and at the moment constitute about 1% of all reported bone fractures. They appear mainly as a result of traffic accidents, falls from heights, as well as being observed in groups of physically active people whose disciplines involve high-impact landings. From time to time, improperly executed movements or steps without falling result in injury. Case Report: We present a case study of a 21-year-old male football player with fracture of the lateral tibia condyle with indentation of articular surface. The …

Assessment Of Fatigue And The Overtraining Syndrome In Female Spinning Instructors Based On A Survey And On The Expression Of Selected Genes, Iwona Bonislawska, Aleksandra Patyna, Jacek Wilczynski, Robert Urbanski, Malgorzata Zychowska

Baltic Journal of Health and Physical Activity

Background: The study aimed to assess the occurrence of fatigue and overtraining in spinning instructors based on subjective feeling (a questionnaire) and the expression of selected genes (IL6, IL10 and NF-kB mRNA). Material/Methods: Two research methods were used in the study: a subjective assessment in the form of a survey and an objective evaluation of the expression of genes related to the cellular stress response. The survey involved a group of 12 female spinning instructors 24–32 years old. Five subjects (mean age 26.4 years) agreed to have a blood sample taken for genetic analyses. The control group comprised four physically …

Validation Of Accelerometer For Measuring Physical Activity In Free-Living Individuals, George O. Agogo, Hilko Van Der Voet, Paul J.M. Hulshof, Pieter Van ’T Veer, Laura Trijsburg, Fred A. Van Eeuwijk, Hendriek C. Boshuizen

Baltic Journal of Health and Physical Activity

Background: The aim of this research was to validate a triaxial GT3X accelerometer against doubly labelled water for measuring total energy expenditure (TEE) in a study of free-living Dutch adults and to compare the two prediction equations used to calculate accelerometer derived activity related energy expenditure. Material/Methods: We used a measurement error model to estimate bias in the mean TEE, a correlation coefficient between measured and true TEE (a validity coefficient, which quantifies loss of statistical power to detect association) and the attenuation factor (which quantifies bias in the association), with and without conditioning on age, sex and BMI. We …

Campus Assembly Minutes 03/29/2022, Campus Assembly

Campus Assembly

No abstract provided.

Management Strategies And Distribution Of Aphanomyces Root Rot Of Alfalfa (Medicago Sativa), A Continuing Threat To Forage Production In The United States, Jennifer M. Giles, Conner L. Tordsen, Travis R. Rebstock, Bruna Bucciarelli, Deborah A. Samac, Andrew E. Sathoff

Research & Publications

Alfalfa (Medicago sativa) is one of several legumes that is affected by Aphanomyces root rot (ARR) caused by Aphanomyces euteiches. Symptoms of ARR on alfalfa seedlings include a yellow-grey discolouration of roots, rotting and loss of lateral roots, stunted growth, chlorotic foliage and reduction of nitrogen-producing nodules on roots. Infection can also occur on adult plants leading to loss of lateral roots and nodules. At the seedling stage, ARR decreases alfalfa stand establishment, and field longevity is reduced when adult plants are infected. A. euteiches is an oomycete pathogen that has motile zoospores and thick-walled oospores that …

Informing Management Of Henrys Lake, Idaho, Using An Integrated Catch-At-Age Model, Joshua L. Mccormick, Jennifer Vincent, Brett High, Darcy K. Mccarrick, Michael C. Quist

United States Geological Survey: Staff Publications

Henrys Lake, Idaho, supports a popular fishery for Yellowstone Cutthroat Trout Oncorhynchus clarkii bouvieri and Yellowstone Cutthroat Trout × Rainbow Trout O. mykiss hybrids. A majority of the adult population of fish in Henrys Lake are of hatchery origin that were stocked as fingerlings. The fishery is closed to angling during the late winter and spring months, but fisheries managers are considering opening the fishery year-round with catch-and-release- only regulations or with a two-fish bag limit during the extended season. However, there is concern that the proposed management actions may negatively affect the current fishery. Therefore, we developed an integrated …

Aging-Associated Changes In Motor Function Are Ovarian Somatic Tissue-Dependent, But Berm Cell And Estradiol Independent In Post-Reproductive Female Mice Exposed To Young Ovarian Tissue, Tracy L. Habermehl, Kaden B. Underwood, Kevin D. Welch, Steven P. Gawrys, Kate C. Parkinson, Augusto Schneider, Michal M. Masternak, Jeffrey Mason

Center for Integrated Biosystems Publications

A critical mediator of evolution is natural selection, which operates by the divergent reproductive success of individuals and results in conformity of an organism with its environment. Reproductive function has evolved to support germline transmission. In mammalian ovaries, this requires healthy, active gonad function, and follicle development. However, healthy follicles do not contribute to germline transmission in a dead animal. Therefore, support of the health and survival of the organism, in addition to fertility, must be considered as an integral part of reproductive function. Reproductive and chronological aging both impose a burden on health and increase disease rates. Tremors are …

Curriculum Subcommittee Agenda, April 7, 2022, Utah State University

Educational Policies Committee

1. Approval of 3 March 2022 Minutes
2. Program Proposals
3. Semester Course Approval Reviews https://usu.curriculog.com/
4. Other Business New Curriculum Subcommittee Chair appointment. Acceptance of membership for 2022-2023 academic year.

Program Proposals

• Request from the Department of Plants, Soils and Climate in the College of Agriculture and Applied Sciences to offer a new specialization (Bioinformatics and Computational Biology) to the MS and PhD degrees of Plant Science.
• Request from the Department of Theatre Arts in the Caine College of the Arts to change the name of the Theatre Arts Theatre Education Certification Option BFA to Theatre Arts Education BFA.
• Request from the Department …

A Resilience-Based Intervention To Mitigate The Effect Of Hiv-Related Stigma: Protocol For A Stepped Wedge Cluster Randomized Trial, Xiaoming Li, Shan Qiao, Xueying Yang, Sayward Harrison, Cheuk Chi Tam, Zhiyong Shen, Yuejiao Zhou

Faculty Publications

Background: Despite decades of global efforts to tackle HIV-related stigma, previous interventions designed to reduce stigma have had limited effects that were typically in the small- to-moderate range. The knowledge gaps and challenges for combating HIV-related stigma are rooted both in the complexity of the stigma and in the limitations of current conceptualizations of stigma reduction efforts. Recent research has shown the promise of resilience-based approaches that focus on the development of strengths, competencies, resources, and capacities of people living with HIV (PLWH) and their key supporting systems (e.g., family members and healthcare providers) to prevent, reduce, and mitigate the …

Amphiphilic Cell-Penetrating Peptides Containing Natural And Unnatural Amino Acids As Drug Delivery Agents, David Salehi, Saghar Mozaffari, Khalid Zoghebi, Sandeep Lohan, Dindyal Mandal, Rakesh Tiwari, Keykavous Parang

Pharmacy Faculty Articles and Research

A series of cyclic peptides, [(DipR)(WR)₄], [(DipR)₂(WR)₃], [(DipR)₃(WR)₂], [(DipR)₄(WR)], and [DipR]₅, and their linear counterparts containing arginine (R) as positively charged residues and tryptophan (W) or diphenylalanine (Dip) as hydrophobic residues, were synthesized and evaluated for their molecular transporter efficiency. The in vitro cytotoxicity of the synthesized peptides was determined in human epithelial ovary adenocarcinoma cells (SK-OV-3), human lymphoblast peripheral blood cells (CCRF-CEM), human embryonic epithelial kidney healthy cells (HEK-293), human epithelial mammary gland adenocarcinoma cells (MDA-MB-468), pig epithelial kidney normal cells (LLC-PK1), and human epithelial …

Tumor Suppressor Par-4 Regulates Complement Factor C3 And Obesity, Nathália Araujo, James Sledziona, Sunil K. Noothi, Ravshan Burikhanov, Nikhil Hebbar, Saptadwipa Ganguly, Tripti Shrestha-Bhattarai, Beibei Zhu, Wendy S. Katz, Yi Zhang, Barry S. Taylor, Jinze Liu, Li Chen, Heidi L. Weiss, Daheng He, Chi Wang, Andrew J. Morris, Lisa A. Cassis, Mariana N. Nikolova‑Karakashian, Prabhakara R. Nagareddy, Olle Melander, B. Mark Evers, Phillip A. Kern, Vivek M. Rangnekar

Clinical and Translational Science Faculty Publications

Prostate apoptosis response-4 (Par-4) is a tumor suppressor that induces apoptosis in cancer cells. However, the physiological function of Par-4 remains unknown. Here we show that conventional Par-4 knockout (Par-4^-/-) mice and adipocyte-specific Par-4 knockout (AKO) mice, but not hepatocyte-specific Par-4 knockout mice, are obese with standard chow diet. Par-4^-/- and AKO mice exhibit increased absorption and storage of fat in adipocytes. Mechanistically, Par-4 loss is associated with mdm2 downregulation and activation of p53. We identified complement factor c3 as a p53-regulated gene linked to fat storage in adipocytes. Par-4 re-expression in adipocytes or c3 deletion reversed …

As-937-22 Resolution On New Degree Program For Masters Of Science In Statistics, Andrew Schaffner, Kelly Bodwin, John Walker, Statistics Department

Academic Senate Resolutions

Approves the new degree program for final review by the Chancellor’s Office.

Executive Committee - Agenda 3/29/2022, Academic Senate

Academic Senate Agendas

No abstract provided.

A Resilience-Based Intervention To Mitigate The Effect Of Hiv-Related Stigma: Protocol For A Stepped Wedge Cluster Randomized Trial, Xiaoming Li Ph.D., Shan Qiao Ph.D., Xueying Yang, Sayward E. Harrison, Cheuk Chi Tam, Zhiyong Shen, Yuejiao Zhou

Faculty Publications

Background: Despite decades of global efforts to tackle HIV-related stigma, previous interventions designed to reduce stigma have had limited effects that were typically in the small- to-moderate range. The knowledge gaps and challenges for combating HIV-related stigma are rooted both in the complexity of the stigma and in the limitations of current conceptualizations of stigma reduction efforts. Recent research has shown the promise of resilience-based approaches that focus on the development of strengths, competencies, resources, and capacities of people living with HIV (PLWH) and their key supporting systems (e.g., family members and healthcare providers) to prevent, reduce, and mitigate the …

March 29, 2022 Ereporter, University Of Alabama At Birmingham

eReporter (campus newsletter)

No abstract provided.

Improving Robustness Of Deep Learning Models And Privacy-Preserving Image Denoising, Hadi Zanddizari

USF Tampa Graduate Theses and Dissertations

Applications of deep learning models and convolutional neural networks have been rapidly increased. Although state-of-the-art CNNs provide high accuracy in many applications, recent investigations show that such networks are highly vulnerable to adversarial attacks. The black-box adversarial attack is one type of attack that the attacker does not have any knowledge about the model or the training dataset, but it has some input data set and theirlabels.

In this chapter, we propose a novel approach to generate a black-box attack in a sparse domain, whereas the most critical information of an image can be observed. Our investigation shows that large …