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Sensitivity Of A Lamp Assay For Detection Of The Dinoflagellate Amyloodinium Ocellatum In Simulated Field Conditions And Freeze Tolerance Of The Parasite, Robert Gonzales

Master's Theses

The obligate parasitic dinoflagellate Amyloodinium ocellatum causes amyloodiniosis in warm water marine fishes. The prolific parasite, which has a direct, three-stage life cycle, is highly infectious and can cause heavy losses in aquaculture. Prevention, biosecurity, and early detection are vital for control. In this work, microscopy and a loop-mediated isothermal amplification (LAMP) assay were compared for early diagnosis of A. ocellatum in cultured stocks, and the freeze tolerance of tomonts was assessed to determine if frozen wild fish used as fish food can serve as a potential vector for the parasite.

The lowest dinospore concentration that could be detected by …

High-Throughput Methodology To Identify Crispr-Generated Danio Rerio Mutants Using Fragment Analysis With Unmodified Pcr Products, Sarah Colijn, Ying Yin, Amber N Stratman

2020-Current year OA Pubs

Targeted mutagenesis in zebrafish, fruit flies, and C. elegans has been significantly improved over the years through CRISPR technology. CRISPR enables researchers to efficiently examine cellular pathways by inducing small, targeted mutations in vivo. Though these mutations are commonly random insertions or deletions (indels), they often result in functionally disrupted alleles of a target gene if the CRISPR components are appropriately designed. However, current protocols used to identify the presence of CRISPR-generated indels are often labor intensive, time-consuming, or expensive. Here, we describe a straightforward, high-throughput method for identifying the presence of mutations by using a fragment analyzer platform which …

Association Of Prenatal Exposure To Early-Life Adversity With Neonatal Brain Volumes At Birth, Regina L Triplett, Rachel E Lean, Amisha Parikh, J Philip Miller, Dimitrios Alexopoulos, Sydney Kaplan, Dominique Meyer, Christopher Adamson, Tara A Smyser, Cynthia E Rogers, Deanna M Barch, Barbara Warner, Joan L Luby, Christopher D Smyser

2020-Current year OA Pubs

Importance: Exposure to early-life adversity alters the structural development of key brain regions underlying neurodevelopmental impairments. The association between prenatal exposure to adversity and brain structure at birth remains poorly understood.

Objective: To examine whether prenatal exposure to maternal social disadvantage and psychosocial stress is associated with neonatal global and regional brain volumes and cortical folding.

Design, Setting, and Participants: This prospective, longitudinal cohort study included 399 mother-infant dyads of sociodemographically diverse mothers recruited in the first or early second trimester of pregnancy and their infants, who underwent brain magnetic resonance imaging in the first weeks of life. Mothers were …

Thpok Inhibits Osteoclast Formation Via Nfatc1 Transcription And Function, Wei Zou, Takashi Izawa, Nidhi Rohatgi, Steven Y Zou, Yongjia Li, Steven L Teitelbaum

2020-Current year OA Pubs

Both LRF (

Troponin Is Unrelated To Outcomes In Heart Failure Patients Discharged From The Emergency Department, Gregory J Fermann, Douglas Char, Et Al

2020-Current year OA Pubs

BACKGROUND: Prior data has demonstrated increased mortality in hospitalized patients with acute heart failure (AHF) and troponin elevation. No data has specifically examined the prognostic significance of troponin elevation in patients with AHF discharged after emergency department (ED) management.

OBJECTIVE: Evaluate the relationship between troponin elevation and outcomes in patients with AHF who are treated and released from the ED.

METHODS: This was a secondary analysis of the Get with the Guidelines to Reduce Disparities in AHF Patients Discharged from the ED (GUIDED-HF) trial, a randomized, controlled trial of ED patients with AHF who were discharged. Patients with elevated conventional …

Capacity Development In The Ocean Decade And Beyond: Key Questions About Meanings, Motivations, Pathways, And Measurements, H Harden-Davies, Dj Amon, M Vierros, Nj Bax, Et Al

School of Biological and Marine Sciences

Capacity development is a major priority in the United Nations Decade of Ocean Science for Sustainable Development (the Decade). Persistent disparities in ocean science capacity illustrate the substantial challenges to achieving the Decade's stated goal of eradicating inequality. We argue that a new conversation about capacity development is essential for the success of the Decade and beyond. We question the meaning, motivations, pathways and measurement of capacity development at this critical juncture. While we do not propose a single answer to these context- and situation-specific questions, we do recognize that the lack of accepted, or even defined, approaches to capacity …

The Exceptional Weather Of April 2021, Jfp Galvin, Mp Fuller

School of Biological and Marine Sciences

April 2021 brought exceptional weather to the British Isles. It was characterised by sunny and unusually dry conditions with minimum temperatures much lower than normal. There was drought in many southern areas and late snows were a feature, especially in the north. This paper summarises the climate of April in Ireland, Great Britain and the Crown Dependencies of Guernsey, Jersey and the Isle of Man using data from both official (state-run or state-maintained) stations, supplemented by data from amateur sites, notably those of the Climatological Observers Link. The weather in early spring 2021 affected the growth of many crops and …

‘It’S Not Just Music, It Helps You From Inside’: Mixing Methods To Understand The Impact Of Music On Young People In Contact With The Criminal Justice System, L Caulfield, A Jolly, E Simpson, Y Devi-Mcgleish

School of Health Professions

In response to some of the criticisms of previous research into the arts in criminal justice, this article presents findings from research with a music programme run by a Youth Offending team (YOT). Data were collected on the attendance of 42 participants at YOT appointments – matched against a comparison group – and measures of change over time in musical development, attitudes and behaviour and well-being. Participants who completed the music programme were statistically more likely to attend YOT appointments than a comparison group. There were statistically significant improvements in participants’ self-reported well-being and musical ability over the course of …

Understanding How To Promote Healthy Dietary Habits Among Parents Of Adolescents, Jazmine D. Collins

Electronic Theses and Dissertations

Adolescents in the United States today have record-high prevalence rates for childhood obesity and overweight, which are conditions that have lifelong negative health consequences for individuals, and also carry high financial and social costs for the entire nation. The disproportionate burden of obesity and associated health conditions falls on adolescents of racial and ethnic minority group identities and from lower-income households. The increased risk that adolescents have for developing conditions like childhood obesity is closely associated with youth having diets that are unhealthier than in the past. Parents have a large influence on the eating habits and preferences of their …

How Early Education And Pet Therapy May Help Nurses With Compassion Fatigue, Katie E. Clark

Electronic Theses and Dissertations

Abstract

Although there is a great deal of research on the causes of compassion fatigue, there is little research on prevention techniques or ways to combat compassion fatigue. The purpose of this qualitative phenomenological study was to discover if nurses are prepared to cope with occupational stress and compassion fatigue. A secondary purpose was to examine the perceived benefit that pet therapy can have as a possible stress reduction technique to decrease the effects of compassion fatigue. This research was conducted by gathering data via semistructured interviews. The researcher interviewed nurses who were currently practicing patient care and scored as …

Pastoral Counseling Marriage Ministry, Chris Schroeder

Doctoral Dissertations and Projects

Marriage ministry is the topic for this thesis project. Several aspects of marriage ministry at First Baptist Church of Texarkana were evaluated. First, a young single adult group at the church participated in a curriculum for dating before marriage. After being taught the reasons for marriage and dating, the class filled out a survey to evaluate the class’ effectiveness. Next, the pre-marriage counseling at FBC Texarkana was evaluated for effectiveness. Third, crisis marriage counseling at FBC Texarkana was evaluated by the writer to determine if marriages were finding healing. Fourth, a marriage class was offered at FBC Texarkana for a …

Aging Voices In The American Church Choir: A Study In Ethnomusicology And Ethnodoxology, Shirley Claudeen Ehler

Doctoral Dissertations and Projects

Despite American church choirs being a distinct culture across all denominations and independent church entities, this traditional worship community of singers is a minority culture in the United States. Furthermore, existing journal literature frequently features young voices, e.g., children's, middle school, teen, and university choirs and choruses. Aging-voice research literature began to appear in the 1990s for individual voices or voices in community choirs. Aging voices are a unique ethnicity found in every race within the church choir culture. Researched literature on the aging voice in the American church choir is conspicuous by its absence, pushing American church choir aging …

God-Image And Romantic Partner Relationships: Emotional Neglect And Adult Women, Danette Alene Buchanan

Doctoral Dissertations and Projects

Childhood emotional neglect often leads to relationship dissatisfaction, psychopathology, decline in physical health, and permanent scars from a lack of emotional validation. Women who have grown up with a history of emotional invalidation are at risk for suicide, divorce, emotional instability, unstable relationships, and a chronic belief that they are not enough. Lacking emotional attachment from parents and other primary figures, girls and young women may transition into adulthood either anxiously attached, avoidant attached, or both. This state of dysphoria may extend into their perceptions of God and other authority figures. Counselors and other professional helpers such as clergy, teachers, …

The Impact Of Father Absence On The Marital Satisfaction Of African American Women, Ashley Ann Sanders

Doctoral Dissertations and Projects

The African American community is plagued with single-parent homes, headed by women. Importantly, while the father-absent phenomenon has gained more attention, much research is still needed to gain a complete perspective of the absent father and its influence upon the adult child, specifically the adult daughter who is at significant risk of continuing in her parent's footsteps. The purpose of this quantitative survey method research is to explore the impact of father absenteeism among African American adult daughters and their marital satisfaction; additionally, examining can supportive mothers moderate the effect. This study examined the marital satisfaction among African American women …

Case Study: Exploration Into Principal's Educational Leadership Training And Their Preparation To Lead Special Education Programs In Schools, Tyriq D'Shon Simmons

Doctoral Dissertations and Projects

The purpose of this case study was to explore the educational leadership training experiences in special education for school principals of public charter schools in Washington D.C. Guiding this study, is the theory of pedagogical content knowledge (PCK), contributed by Shulman (1986), and the social constructivist theory contributed by Lev Vygotsky (1978). These theories seek to explain the relationship between what principals are expected to know and do based on leadership content knowledge and practical application, specifically in terms of leading special education programs in schools. The central question of this study is: What are the experiences of school principals …

Mentorship Relationships: A Phenomenological Study Investigating Experiences Of Secondary Teachers, Jodi Lee Wilson

Doctoral Dissertations and Projects

The purpose of this transcendental phenomenological study was to investigate teachers’ experiences in their mentoring relationships. This study investigated the experiences of 12 secondary teachers with varying levels of teaching experience from the Camel County School District in northeastern Wyoming. Kram’s (1983) original mentorship model guided this study, along with social network theory. The focus of this inquiry was to explore how teachers experience the initiation and cultivation phase of the mentorship relationship within their schools, and how these mentorship relationships positively affect the teachers involved. Data collection methods included interviews, participant-written essays, and focus group sessions to answer the …

Date-Leaf Carbon Particles For Green Enhanced Oil Recovery, Bashirul Haq, Md Abdul Aziz, Dhafer Al Shehri, Nasiru Salahu Muhammed, Shaik Inayath Basha, Abbas Saeed Hakeem, Mohammed Ameen Ahmed Qasem, Mohammed Lardhi, Stefan Iglauer

Research outputs 2022 to 2026

Green enhanced oil recovery (GEOR) is an environmentally friendly enhanced oil recovery (EOR) process involving the injection of green fluids to improve macroscopic and microscopic sweep efficiencies while boosting tertiary oil production. Carbon nanomaterials such as graphene, carbon nanotube (CNT), and carbon dots have gained interest for their superior ability to increase oil recovery. These particles have been successfully tested in EOR, although they are expensive and do not extend to GEOR. In addition, the application of carbon particles in the GEOR method is not well understood yet, requiring thorough documentation. The goals of this work are to develop carbon …

Application Of Red Cabbage Anthocyanins As Ph-Sensitive Pigments In Smart Food Packaging And Sensors, Reza Abedi-Firoozjah, Shima Yousefi, Mahshid Heydari, Faezeh Seyedfatehi, Shima Jafarzadeh, Reza Mohammadi, Milad Rouhi, Farhad Garavand

Research outputs 2022 to 2026

Anthocyanins are excellent antioxidant/antimicrobial agents as well as pH-sensitive indicators that provide new prospects to foster innovative smart packaging systems due to their ability to improve food shelf life and detect physicochemical and biological changes in packaged food. Compared with anthocyanins from other natural sources, red cabbage anthocyanins (RCAs) are of great interest in food packaging because they represent an acceptable color spectrum over a broad range of pH values. The current review addressed the recent advances in the application of RCAs in smart bio-based food packaging systems and sensors. This review was prepared based on the scientific reports found …

18 Fdg Pet/Ct Imaging In Carcinoma Cesophagus, Maseeh Uz Zaman, Nosheen Fatima

Department of Radiology

No abstract provided.

Active Leisure And Ageing In Rural Ireland: Exploring Perceptions And Motivations To Facilitate And Promote Meaningful Physical Activity, Angie Hartnett, Catherine Gorman

Articles

The life expectancy of the world population is increasing and the art of aging well is of global interest (European Commission, n.d.). In Ireland, the number of people over the age of 60 will increase from 1.1 million currently, to 2.44 million by 2041, constituting nearly one third of its total population (Institute of Public Health, 2018). Despite the benefits of regular physical activity (pa), particularly with the frail (≥65 years and requiring a walking aid), rates of participation remain low (Colley et al., 2011). Health concerns raised, such as smoking and weight issues, are more prevalent in this cohort …

Is Breast Conservation Superior To Mastectomy In Early Stage Triple Negative Breast Cancer?, Joseph Panoff

All Publications

Purpose: Compare overall survival (OS) and breast cancer-specific survival (BCSS) outcomes of breast conservative therapy (BCT) and mastectomy in a large cohort of patients with early-stage triple negative breast cancer (TNBC), using a propensity score-based matching approach.

Methods: Surveillance, Epidemiology, and End Results (SEER) database was used to study the role of RT in early stage TNBC. Primary end points were OS and BCSS. Cox proportional hazard regression models and Kaplan-Meier plots were used to generate the desired outcomes. Propensity score matching was done to minimize bias.

Results: 12,761 patients with T1-2N0M0 TNBC as their first malignancy were retrieved. Of …

Mitophagy-Mediated Regulation Of Ros Homeostasis During Hsc Activation Contributes To Cell Fate, Amber Lynne Smith

Theses and Dissertations (ETD)

The ability of hematopoietic stem cells to self-renew and differentiate is required for the maintenance of all blood lineages under basal physiologic conditions and in response to inflammatory stress. The degradative capacity of the autophagy-lysosomal system is a determinant of hematopoietic stem cell (HSC) activation status with low autophagy predicting activation of HSCs, and autophagy deficiency causing the spontaneous loss of quiescence and HSC exhaustion. Although an increase in mitochondrial activity has been suggested as the cause of HSC depletion in autophagy-defective models, the contribution of mitophagy to HSC function remains to be elucidated. Here, we capitalized on the observation …

Cellular Dynamics And Disease Outcome Of Type 3 Streptococcus Pneumoniae Clinical Isolates Differ Between Strains, Taylor Rae Plunkett White

Theses and Dissertations (ETD)

Streptococcus pneumoniae is a bacterial pathogen that continues to be a major cause of disease around the world. It is not only the number one cause of bacterial pneumonia but also the cause of about 15% of the deaths of children under 5 around the world. There is a lot of research done on this organism, but with around 100 known serotypes and each one producing a unique capsule, there is still much more to be studied. The Etiology of Pneumonia in the Community (EPIC) study conducted by the CDC observed the burden of hospitalizations caused by pneumonia while determining …

A Rodent Animal Model For Forelimb To Lower Jaw Reorganization In Primary Somatosensory Cortex (Si) Barrel Field, Violeta Pellicer Morata

Theses and Dissertations (ETD)

The goal of this study is to examine mechanisms underlying cortical reorganization in rat primary somatosensory (SI) cortex that follows forelimb deafferentation. The majority of human patients suffering from limb loss or brachial plexus avulsion injury, as well as patients that receive brachial plexus anesthesia, report phantom limb sensations/pain which are often associated with cortical reorganization. Patients with upper-limb deafferentation report sensations of the missing hand during tactile stimulation of the face, and this phenomenon has been termed hand-to-face remapping. We sought to develop a rodent model of deafferentation; our model system is the rat SI cortex barrel field present …

Targeting Protein Degradation To Uncover Novel Oncoprotein Drivers Of Acute Leukemia, Fatemeh Keramatnia

Theses and Dissertations (ETD)

Acute Lymphoblastic Leukemia (ALL), the most common childhood cancer and the second most common acute leukemia in adults, arises from clonal expansion of undifferentiated lymphoid precursor cells in bone marrow. Despite the extensive knowledge on its cytogenetic and molecular biology, ALL treatment remains highly challenging especially after relapse. Conventional chemotherapy has shown significant improvement in overall survival rates of pediatric patients up to 90%, however, treatment failure due to ALL relapse occurs in 15-20% of the cases. On the other hand, adults and elderly patients with ALL are considered difficult to treat populations with the 5-year overall survival of 30–40%. …

The Relationship Of Child Health And Parenting Stress To Child Neurocognitive Development, Nancy Alise Farrell

Theses and Dissertations (ETD)

Problem. Although the United States rates high in economic resources, the neurocognitive development (NCD) of children falls behind many other developed nations. Levels of parenting stress may be linked to sociodemographic factors such as the high incidence of poverty in children or health disparities in children, which in turn may affect NCD in children. Using the data from an observational, longitudinal cohort study of a diverse population could provide information related to parenting stress, child health, and related factors to guide and support interventions with families and policies to improve child NCD. Aims. The overall aim of this study was …

Immunomodulatory Roles Of The Lysosomal Sialidase Neuraminidase 1, Leigh Ellen Fremuth

Theses and Dissertations (ETD)

Background Sialic acids are key sugar moieties located at the non-reducing terminals of glycan chains on glycoproteins and glycolipids. By virtue of their location, they influence the functions and biochemical properties of the macromolecules they are bound to. Removal of sialic acids in mammalian cells is carried out by four sialidases, which are differentially expressed and localized in distinct subcellular compartments. Neuraminidase 1 (NEU1), the most abundant and ubiquitous of the four sialidases, functions primarily in the acidic environment of the lysosomes, but can hydrolyze substrates at the plasma membrane, at least in certain cell types. The enzyme initiates the …

Developing Targeted Therapies For T-Cell Acute Lymphoblastic Leukemia, Jianzhong Hu

Theses and Dissertations (ETD)

Introduction Acute lymphoblastic leukemia (ALL) is the most common cancer in children, and risk-adapted chemotherapies have dramatically improved the outcomes of this disease. Compared to B-cell ALL, T-cell ALL (T-ALL) is more aggressive and has worse outcomes from chemotherapy. There is a great unmet need to develop biomarkers and novel targeted therapies for this type of cancer. Working together with internal and external collaborators, we performed a large-scale pharmacotyping assay in over 300 primary ALL samples. By combining pharmacotypying and genomic profiling of these samples, we identified a substantial T-ALL population that showed strong response to dasatinib, a known ABL1 …

Substituent Effect Analysis Of The Synthesis Of 1-Azabicyclo[2.2.1]Heptan-2-One Using Diels-Alder Reactions, Vida Robertson

Honors Theses

No abstract provided.

Cna Status As An Admission Criterion For Undergraduate Nursing Students: A Comparative Case Study, Nancy Capponi

Dissertations

Undergraduate nursing programs continue to be unable to meet the current and projected demand for nurses. Faculty and administrators need to choose admission criteria that best predict student success in the program to provide newly graduated nurses to meet societal demands for nurses. The objective for this study was to investigate the perceptions of clinical faculty and undergraduate nursing students regarding effects of the nonacademic admission criterion of status as a Certified Nursing Assistant (CNA) on first-year student preparedness for the clinical setting, self-confidence and anxiety in clinical decision-making (CDM), and student success. A comparative case study design was used …