Digital Commons Network^™

Neither Wind Nor Ice Could Stop Kelly Loves Fieldwork, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

What Emily Marino Didn't Expect To Find In The Lab, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

April 6, 2022 Greenmail, University Of Alabama At Birmingham

GreenMail (student newsletter through 2024)

No abstract provided.

Feasibility Of Tubulin As A Control For Gene Expression Following Transfection In Mouse Monocyte/Macrophage-Like Cells, Ankita Chabra

Honors Program Theses and Research Projects

Transfection, which is the ability to modify host cells’ genetic content, has broad application in studying normal cellular processes, molecular mechanism of disease and gene therapy. There are several transfection techniques, and all require either a control or a reference gene. Commonly used controls for transfection experiments are housekeeping genes, which maintain expression for a given cell/tissue, experimental conditions, and treatment. However, recent research has uncovered that expression levels of housekeeping genes may vary depending on the gene, cell type and experimental conditions. A growing body of evidence demonstrates that housekeeping genes are inadequate internal standards for measuring gene expression …

Upper Missouri Waterkeeper V. Epa, Clare Ols

Public Land & Resources Law Review

State water quality standards developed under the Clean Water Act play a key role in curtailing the negative environmental, economic, and human health impacts of water pollution. Under the state water quality regulatory framework, EPA may grant variances to state standards should the state demonstrate the compliance with its standards is infeasible for a certain pollutant discharger or waterbody. Montana DEQ developed a variance for nutrients based on evidence that compliance with those standards would cause economic harm. EPA approved Montana's nutrient pollutant variance, and Upper Missouri Waterkeeper challenged EPA's approval on the grounds that the variance violates the Clean …

A Vaccine Education Module For University Students: Vaccine Concerns And Knowledge Gained, Joslyn D. Wilson

Theses and Dissertations

Purpose: The purposes of this research are to evaluate the effectiveness of a vaccine education module (VEM) in 1) contributing to new vaccine knowledge of university freshmen; and 2) identifying common vaccine concerns of university freshmen before and after completing the VEM. Methods: A VEM, including information about vaccine-preventable diseases, benefits of vaccines, principles of community or herd immunity, and where to go to get vaccinated, was created for freshman students. Student vaccine concerns were identified using a pretest/posttest quasi-experimental design using an online questionnaire before and after completing the VEM. After the VEM, participants were also asked to identify …

Cherry Red Greenwashing: The Rhetoric Behind Corporate Recycling Narratives, Jessica Wallace Haws

Theses and Dissertations

As the public becomes more aware of environmental issues, corporations are pressed to consider and address the sustainability of their practices. Unwilling to drastically change business models, many corporations turn to greenwashing in an attempt to construct an environmentally friendly image while doing little to nothing to address sustainability issues. Using Kenneth Burke's work on identification and terministic screens, I analyze The Coca-Cola Company's "2020 World Without Waste Report" to illuminate how consumers come to believe in and identify with corporate greenwashing tactics. In line with Burke's theories related to identification, I argue that Coca-Cola's greenwashing strategies can be categorized …

Laboratory Simulations Of Simultaneous Reduced Gravity And Ionizing Radiation Environments, Achal Duhoon, Jr Dennison

Conference Proceedings

A novel system has been developed to simulate the combined effects of reduced gravity and ionizing radiation present during spaceflight on biological and particulate samples. The miniature rotary cell culture system (mRCCS) was designed to synchronously rotate up to five independent vessels containing particulate samples suspended in fluid media, constructed using radiation tolerant, biocompatible, and vacuum compatible materials. Reduced gravity conditions were achieved when particles (e.g., 200 µm polystyrene microcarrier beads with or without adhered cell clusters) were suspended inside the vessels moving near terminal velocity in viscous fluid media with densities matched to the suspended …

Sources And Types Of Social Supports And Their Association With Mental Health Symptoms And Life Satisfaction Among Young Adults With A History Of Out-Of-Home Care, Rhiannon Evans, Colleen C. Katz, Anthony Fulginiti, Heather N. Taussig

Graduate School of Social Work: Faculty Scholarship

Young adults with a history of out-of-home care report poorer mental health and life satisfaction compared to non-care-experienced peers. Social support is a known protective factor for mental health. There is limited evidence, however, on the relationship between sources (e.g., family members) and types (e.g., information) of social support and mental health symptoms and life satisfaction in this population. Reporting cross-sectional survey data from 215 young adults aged 18–22 years with a history of out-of-home care, the current study conducted descriptive, bivariate, and linear regression analysis to examine the different sources and types of support young adults receive and their …

Brain Charts For The Human Lifespan, R A I Bethlehem, J Seidlitz, S R White, C Ortinau, C D Smyser, Et Al

Open Access Publications

No abstract provided.

Life Beyond Bars: Nine Prisoners And Their Families, And Faith-Based Efforts To Recognize And Avoid-Cross-Generational Criminal Habits., Alfreda Reese

Doctor of Ministry Projects and Theses

The aim of this study is to examine prisoners’ firsthand experiences and their underlying family issues to bring awareness and delete current cross-generational criminal habits. Through analyzing a series of individual experiences and exploring underlying family issues, the study intends to bring awareness and exposure to the implications of the criminal justice system on prisoners and their families. This study will analyze personal stories of prisoners and their families to identify, interact, and intervene in best practices to avoid criminal habits. The research gathered aims to empower prisoners and their families in suggested ways to delete repeated criminal patterns and …

The Bg News April 06, 2022, Bowling Green State University

BG News (Student Newspaper)

The BGSU campus student newspaper. April 06, 2022. Volume 101-Issue 26.

Nanoparticle Quantification And Distribution On Fluorescently Coated Angioplasty Balloons, Allison Dobson Zieschang

Honors Capstones

No abstract provided.

Daily Eastern News: April 06, 2022, Eastern Illinois University

April

No abstract provided.

Lis1 And Ndel1 Regulate Axonal Trafficking Of Mitochondria In Mature Neurons, Jai P. Pandey, Liang Shi, Remi A. Brebion, Deanna S. Smith

Faculty Publications

Defective mitochondrial dynamics in axons have been linked to both developmental and late-onset neurological disorders. Axonal trafficking is in large part governed by the microtubule motors kinesin-1 and cytoplasmic dynein 1 (dynein). Dynein is the primary retrograde transport motor in axons, and mutations in dynein and many of its regulators also cause neurological diseases. Depletion of LIS1, famous for linking dynein deregulation to lissencephaly (smooth brain), in adult mice leads to severe neurological phenotypes, demonstrating post-developmental roles. LIS1 stimulates retrograde transport of acidic organelles in cultured adult rat dorsal root ganglion (DRG) axons but findings on its role in mitochondrial …

The Lumberjack, April 06, 2022

Cal Poly Humboldt's Student Newspaper

The student newspaper of Humboldt State University.

Sex And Starvation Influences Latrotoxin Expression In The Brown Widow Spider, Mattie Harris

Honors College Theses

Widow spiders (genus Latrodectus) are well-known for their potent venom. Seven latrotoxin proteins constitute the main components of widow spider venom. The vertebrate specific (α-latrotoxin) and insect specific (α-latroinsectotoxin) latrotoxins have been well-characterized with respect to structure and function. Regulation of latrotoxin gene expression is not well understood but sex and feeding could be factors influencing production. In this study, I used quantitative qPCR to (1) characterize the expression patterns of both the insect and vertebrate specific latrotoxins in male and female brown widow spiders (Latrodectus geometricus) to characterize sex-biased expression and to (2) study expression patterns …

Following The Science: The Role Of The Academic Medical Center In The Covid-19 Pandemic, Richard L. Summers, Leslie Musshafen, Gailen Marshall, John Bates, Sarika Jain, Jake Johnston, Alexandra Castillo, Krishna Ayyalasomayajula, Ramona Sandlin, I. J. Frame, Seth Lirette, Saurabh Chandra, Ashley Robinson, Michael R. Garrett

Journal of Public Health in the Deep South

This article reviews the experience and role of an academic medical center in a response to the COVID-19 pandemic.

State-Of-The-Art Versus Deep Learning: A Comparative Study Of Motor Imagery Decoding Techniques, Olawunmi Olaboopo George, Sarthak Dabas, Abdur Sikder, Roger O. Smith, Praveen Madiraju, Nasim Yahyasoltani, Sheikh Iqbal Ahamed

Computer Science Faculty Research and Publications

State-of-the-art techniques (SOTA) for motor imagery decoding have largely involved the use of common spatial patterns (CSP) and power spectral density (PSD), for feature extraction. Other frequency transforms, such as wavelets and empirical mode decomposition (EMD) have also been used but the aforementioned two have been the most popular. For classification, linear discriminant analysis (LDA) and support vector machines (SVM) have been mostly used. It is, however, worth investigating other approaches, such as deep learning, which offer a potential for improvement, but are not yet mainstream. Deep learning techniques based on neural networks (NNs) have been underexplored in motor imagery …

Mapping Yellow Fever Epidemics As A Potential Indicator Of The Historical Range Of Aedes Aegypti In The United States, Nicole S. Fijman, Donald A. Yee

Faculty Publications

Background: Yellow fever (YF) plagued the United States from the 1690s until 1905, resulting in thousands of deaths. Within the US, Aedes aegypti is the only YF vector and almost no data exists for the location of this species prior to the early 1900s.

Objectives: To determine the historical range of Ae. aegypti we examined the occurrence of YF epidemics across time and space. We hypothesized that historically Ae. aegypti was driven by human population density, like its contemporary range suggests.

Methods: To test this hypothesis, we compiled a list of YF cases in the US, human population density, location, …

Atg9a And Atg13 Cooperate To Drive Basal Autophagy, Daniel Morgan Poole

Theses and Dissertations

Autophagy, as the name suggests, is a cellular process of self-eating in which cytoplasmic debris is engulfed by a double membrane vesicle dubbed the autophagosome and is ultimately degraded and recycled by proteases in the lysosome. The process is initiated by a group of core ATG proteins, including a multi-pass transmembrane protein called ATG9A. Although ATG9A has been shown to be essential for both stress induced and basal autophagy, its mechanism and interaction network remain largely illusive. Our current study employs BioID proteomics to identify a network of interactors, including regulators of membrane fusion and vesicle trafficking, such as TRAPP, …

How Lucretius Uses Epic, J. Dean Murphy

Theses and Dissertations

I argue that Lucretius uses conventions of epic poetry and changes them to support his philosophical teachings. The proem of De rerum natura can be used to show how this occurs. The key moments within the proem include the invocation to Venus, how Venus is described as maternal and creative, the usage of socia to ask Venus for aid, the episode between Venus and Mars, and the establishment of Epicurean physics. I argue that the purpose of changing the expected conventions of epic is to better frame Lucretius' key idea of the purpose of life, namely reproduction.

Sources And Types Of Social Supports And Their Association With Mental Health Symptoms And Life Satisfaction Among Young Adults With A History Of Out-Of-Home Care, Rhiannon Evans, Colleen C. Katz, Anthony Fulginiti, Heather Taussig

Publications and Research

Young adults with a history of out-of-home care report poorer mental health and life satisfaction compared to non-care-experienced peers. Social support is a known protective factor for mental health. There is limited evidence, however, on the relationship between sources (e.g., family members) and types (e.g., information) of social support and mental health symptoms and life satisfaction in this population. Reporting cross-sectional survey data from 215 young adults aged 18–22 years with a history of out-of-home care, the current study conducted descriptive, bivariate, and linear regression analysis to examine the different sources and types of support young adults receive and their …

Early Pleistocene Large Mammals From Maka’Amitalu, Hadar, Lower Awash Valley, Ethiopia, John Rowan, Ignacio A. Lazagabaster, Christopher J. Campisano, Faysal Bibi, René Bobe, Jean-Renaud Boisserie, Stephen R. Frost, Tomas Getachew, Christopher C. Gilbert, Margaret E. Lewis, Sahleselasie Melaku, Eric Scott, Antoine Souron, Lars Werdelin, William H. Kimbel, Kaye E. Reed

Publications and Research

The Early Pleistocene was a critical time period in the evolution of eastern African mammal faunas, but fossil assemblages sampling this interval are poorly known from Ethiopia ’ s Afar Depression. Field work by the Hadar Research Project in the Busidima Formation exposures (~2.7 – 0.8 Ma) of Hadar in the lower Awash Valley, resulted in the recovery of an early Homo maxilla (A.L. 666-1) with associated stone tools and fauna from the Maka ’ amitalu basin in the 1990s. These assemblages are dated to ~2.35 Ma by the Bouroukie Tuff 3 (BKT-3). Continued work by the Hadar Research Project …

Proceedings Of The 78th Annual Meeting, Midwestern Association Of Graduate Schools

Midwestern Association of Graduate Schools

Table of Contents: 2022 MAGS Annual Meeting

Milwaukee, Wisconsin | April 6–8, 2022 Theme: Student-Centered Graduate Education: Emerging Pathways for Student Success

Wednesday, April 6, 2022

Preconference: New Graduate Administrators Workshop Plenary I – News from CGS: A Focus on Master’s Education

• Empowering Graduate Students as Educators through a Teaching Learning Community
• Maslow’s Needs Hierarchy: A Tool for Designing a Student-Centered Graduate Education Campus
• Onboarding & Orientation: Creating an Environment & Expectations for Success & Well-Being
• Bridging the Gap: Holistic Graduate Student Support at Michigan Tech
• Ethics Training for PhD Students: Can Research Ethics Keep Pace with Research Itself?
• Structuring …

Technology Use In Schizotypy Utilizing The Domains Of Technology Use Survey (Dotus), Devin Massaro

Theses and Dissertations

The diathesis stress model for schizophrenia posits that genetics and prenatal factors confer vulnerability (diathesis) for mental illness which then may be activated through exposure to environmental stressors during life. One component of our contemporary environment involves technology use which has demonstrated risk and beneficial capabilities in regards to the schizophrenia spectrum. Using an online and in-person survey, this study aimed to elucidate the manner in which time spent on technology related to schizotypy, problematic technology use (PTU), and health behaviors in 227 undergraduate students (aged 18-30). We hypothesized that levels of schizotypy, as well as time spent on social …

Hur Plays A Role In Double-Strand Break Repair In Pancreatic Cancer Cells And Regulates Functional Brca1-Associated-Ring-Domain-1(Bard1) Isoforms, Aditi Jain, Matthew Mccoy, Carolyn Coats, Samantha Z Brown, Sankar Addya, Carl Pelz, Rosalie C Sears, Charles Yeo, Jonathan R Brody

Department of Surgery Faculty Papers

Human Antigen R (HuR/ELAVL1) is known to regulate stability of mRNAs involved in pancreatic ductal adenocarcinoma (PDAC) cell survival. Although several HuR targets are established, it is likely that many remain currently unknown. Here, we identified BARD1 mRNA as a novel target of HuR. Silencing HuR caused a >70% decrease in homologous recombination repair (HRR) efficiency as measured by the double-strand break repair (pDR-GFP reporter) assay. HuR-bound mRNAs extracted from RNP-immunoprecipitation and probed on a microarray, revealed a subset of HRR genes as putative HuR targets, including the BRCA1-Associated-Ring-Domain-1 (BARD1) (p < 0.005). BARD1 genetic alterations are infrequent in PDAC, and its context-dependent upregulation is poorly understood. Genetic silencing (siRNA and CRISPR knock-out) and pharmacological targeting of HuR inhibited both full length (FL) BARD1 and its functional isoforms (α, δ, Φ). Silencing BARD1 sensitized cells to olaparib and oxaliplatin; caused G2-M cell cycle arrest; and increased DNA-damage while decreasing HRR efficiency in cells. Exogenous overexpression of BARD1 in HuR-deficient cells partially rescued the HRR dysfunction, independent of an HuR pro-oncogenic function. Collectively, our findings demonstrate for the first time that BARD1 is a bona fide HuR target, which serves as an important regulatory point of the transient DNA-repair response in PDAC cells.

Sex Differences In The Fecal Microbiome And Hippocampal Glial Morphology Following Diet And Antibiotic Treatment, Anju Saxena, Roberta R M Moran, Meghan R. Bullard, Emma O. Bondy, Matthew Foster Smith, Lainie Morris, Nicaella Fogle, Jagroop Singh, Brendan Jarvis, Tammy Ray, Juhi Saxena, Linnea Ruth Freeman

Open Access Fund Publications

Rising obesity rates have become a major public health concern within the United States. Understanding the systemic and neural effects of obesity is crucial in designing preventive and therapeutic measures. In previous studies, administration of a high fat diet has induced significant weight gain for mouse models of obesity. Interestingly, sex differences in high-fat diet-induced weight gain have been observed, with female mice gaining significantly less weight compared to male mice on the same high-fat diet. It has also been observed that consumption of a high-fat diet can increase neurogliosis, but the mechanism by which this occurs is still not …

40-Years Of Lake Urmia Restoration Research: Synthesis And Next Steps, Marsoud Parsinejad, David E. Rosenberg, Yusuf Alizade Govarchin Ghale, Bahram Khazaei, Sarah Null, Omid Raja, Ammar Safaie, Somayeh Sima, Armin Sorooshian, Wayne A. Wurtsbaugh

Lake Urmia, Iran Collaborations

Public concern over environmental issues such as ecosystem degradation is high. However, restoring coupled human-natural systems requires integration across many science, technology, engineering, management, and governance topics that are presently fragmented. Here, we synthesized 544 peer-reviewed articles published through September 2020 on the desiccation and nascent recovery of Lake Urmia in northwest Iran. We answered nine questions of scientific and popular interest about causes, impacts, stabilization, recovery, and next steps. We find: (1) Expansion of irrigated agriculture, dam construction, and mismanagement impacted the lake more than temperature increases and precipitation decreases. (2) Aerosols from Lake Urmia's exposed lakebed are negatively …

Genetic Characterization Of Resistance To Bacterial Panicle Blight And Sheath Blight In Rice Using Qtl Linkage Analysis And Qtl-Seq, John Christian E. Ontoy

LSU Master's Theses

Bacterial panicle blight (BPB) and sheath blight (SB) are major diseases of rice causing significant yield reduction in conducive conditions. Few cultivars have shown reliable resistance to these diseases. In this study, a recombinant inbred line (RIL) population derived from the U.S. cultivars Jupiter (moderately resistant) and Trenasse (susceptible) was investigated to identify genomic regions associated with BPB and SB resistance. Phenotypes in BPB and SB, as well as days to heading (DH), of the RIL population were evaluated in the field. DH was found to be correlated to BPB and SB diseases. BPB was also positively correlated to SB. …