Digital Commons Network^™

An Efficient Strategy For Evaluating New Non-Invasive Screening Tests For Colorectal Cancer: The Guiding Principles, Robert S Bresalier, Carlo Senore, Graeme P Young, James Allison, Robert Benamouzig, Sally Benton, Patrick M M Bossuyt, Luis Caro, Beatriz Carvalho, Han-Mo Chiu, Veerle M H Coupé, Willemijn De Klaver, Clasine Maria De Klerk, Evelien Dekker, Sunil Dolwani, Callum G Fraser, William Grady, Lydia Guittet, Samir Gupta, Stephen P Halloran, Ulrike Haug, Geir Hoff, Steven Itzkowitz, Tim Kortlever, Anastasios Koulaouzidis, Uri Ladabaum, Beatrice Lauby-Secretan, Mārcis Leja, Bernard Levin, Theodore Robert Levin, Finlay Macrae, Gerrit A Meijer, Joshua Melson, Colm O'Morain, Susan Parry, Linda Rabeneck, David F Ransohoff, Roque Sáenz, Hiroshi Saito, Silvia Sanduleanu-Dascalescu, Robert E Schoen, Kevin Selby, Harminder Singh, Robert J C Steele, Joseph J Y Sung, Erin Leigh Symonds, Sidney J Winawer, Members Of The World Endoscopy Colorectal Cancer Screening New Test Evaluation Expert Working Group

Faculty, Staff and Student Publications

OBJECTIVE: New screening tests for colorectal cancer (CRC) are rapidly emerging. Conducting trials with mortality reduction as the end point supporting their adoption is challenging. We re-examined the principles underlying evaluation of new non-invasive tests in view of technological developments and identification of new biomarkers.

DESIGN: A formal consensus approach involving a multidisciplinary expert panel revised eight previously established principles.

RESULTS: Twelve newly stated principles emerged. Effectiveness of a new test can be evaluated by comparison with a proven comparator non-invasive test. The faecal immunochemical test is now considered the appropriate comparator, while colonoscopy remains the diagnostic standard. For a …

Zyxin Inhibits The Epithelial-Mesenchymal Transition Process In Gastric Cancer By Upregulating Sirt1, Jing Lou, Sha Geng, Wei He, Song-Bai Liu, Xinghong Shi, Ying Chang, Shiyuan Han, Panting Qian, Hesham M Amin, Yao-Hua Song, Yangxin Li, Jin Zhou

Faculty, Staff and Student Publications

Tumor development relies on the stemness of cancer stem cells, which is regulated by environmental cues. Previous studies have shown that zyxin can inhibit the expression of genes for embryonic stem cell status. In the present study, the expression levels of zyxin protein in cancer tissues and adjacent noncancerous tissues from 73 gastric cancer patients with different clinical stages were analyzed by Western blot. We showed that the relative expression levels of zyxin in gastric cancer tissues (cancer tissues/adjacent tissues) were significantly downregulated in advanced clinical stages. Overexpression of zyxin inhibited the stemness and epithelial-mesenchymal transition (EMT) processes in gastric …

Therapeutic Effects Of Combining Curcumin And Swimming In Osteoarthritis Using A Rat Model, Mona M Saber, Manal Moustafa Mahmoud, Hesham M Amin, Reham M Essam

Faculty, Staff and Student Publications

Osteoarthritis (OA) is a common debilitating degenerative disease of the elderly. We aimed to study the therapeutic effects of combining curcumin and swimming in monosodium iodoacetate (MIA)-induced OA in a rat model. The rats were divided into 5 groups (n = 9). Group 1 received saline and served as a control group. Groups 2-5 were injected intra-articularly in the right knee with 100 μL MIA. One week later, groups 3 and 5 were started on daily swimming sessions that gradually increased to 20-mins per session, and for groups 4 and 5, oral curcumin was administered at a dose of 200 …

Evaluation Of The Natural History Of Disease By Combining Incident And Prevalent Cohorts: Application To The Nun Study, Daewoo Pak, Jing Ning, Richard J Kryscio, Yu Shen

Faculty, Staff and Student Publications

The Nun study is a well-known longitudinal epidemiology study of aging and dementia that recruited elderly nuns who were not yet diagnosed with dementia (i.e., incident cohort) and who had dementia prior to entry (i.e., prevalent cohort). In such a natural history of disease study, multistate modeling of the combined data from both incident and prevalent cohorts is desirable to improve the efficiency of inference. While important, the multistate modeling approaches for the combined data have been scarcely used in practice because prevalent samples do not provide the exact date of disease onset and do not represent the target population …

Young-Onset Rectal Cancer: Unique Tumoral Microbiome And Correlation With Response To Neoadjuvant Therapy, Michael G White, Ashish Damania, Jumanah Alshenaifi, Pranoti Sahasrabhojane, Oliver Peacock, Jillian Losh, Matthew C Wong, Zuzana Lutter-Berkova, George J Chang, Andrew Futreal, Jennifer A Wargo, Nadim J Ajami, Scott Kopetz, Y Nancy You

Faculty, Staff and Student Publications

OBJECTIVE: External exposures, the host, and the microbiome interact in oncology. We aimed to investigate tumoral microbiomes in young-onset rectal cancers (YORCs) for profiles potentially correlative with disease etiology and biology.

BACKGROUND: YORC is rapidly increasing, with 1 in 4 new rectal cancer cases occurring under the age of 50 years. Its etiology is unknown.

METHODS: YORC (old) or later-onset rectal cancer (LORC, ≥50 y old) patients underwent pretreatment biopsied of tumor and tumor-adjacent normal (TAN) tissue. After whole genome sequencing, metagenomic analysis quantified microbial communities comparing tumors versus TANs and YORCs versus LORCs, controlling for multiple testing. Response to …

Peripheral Levels Of The Brain-Derived Neurotrophic Factor In Coronary Artery Disease: A Systematic Review And Meta-Analysis, Parnian Shobeiri, Amir Hossein Behnoush, Amirmohammad Khalaji, Antonio Lucio Teixeira, Nima Rezaei

Faculty, Staff and Student Publications

BACKGROUND: Among its functions, brain-derived neurotrophic factor (BDNF) regulates endothelial and macrophage activation, possibly playing a role in atherosclerotic plaque pathophysiology. Given contradicting reports, this study sought to investigate whether blood levels of BDNF differed between patients with coronary heart disease (CHD) and controls.

METHODS: We explored PubMed, Embase, Web of Science, and Cochrane Library for studies comparing BDNF blood levels in patients with CHD and controls. Random-effect meta-analysis was conducted to calculate the standardized mean differences (SMD) and 95% confidence intervals (CI). The Newcastle-Ottawa scale was used to evaluate the quality of included articles, and statistical analyses were conducted …

Refphase: Multi-Sample Phasing Reveals Haplotype-Specific Copy Number Heterogeneity, Thomas B K Watkins, Emma C Colliver, Matthew R Huska, Tom L Kaufmann, Emilia L Lim, Cody B Duncan, Kerstin Haase, Peter Van Loo, Charles Swanton, Nicholas Mcgranahan, Roland F Schwarz

Faculty, Staff and Student Publications

Most computational methods that infer somatic copy number alterations (SCNAs) from bulk sequencing of DNA analyse tumour samples individually. However, the sequencing of multiple tumour samples from a patient's disease is an increasingly common practice. We introduce Refphase, an algorithm that leverages this multi-sampling approach to infer haplotype-specific copy numbers through multi-sample phasing. We demonstrate Refphase's ability to infer haplotype-specific SCNAs and characterise their intra-tumour heterogeneity, to uncover previously undetected allelic imbalance in low purity samples, and to identify parallel evolution in the context of whole genome doubling in a pan-cancer cohort of 336 samples from 99 tumours.

Innately Expressed Estrogen-Related Receptors In The Skeletal Muscle Are Indispensable For Exercise Fitness, Ashok Kumar, Vihang A Narkar

Faculty, Staff and Student Publications

Peripheral arterial disease (PAD) is a prevalent cardiovascular complication of limb vascular insufficiency, causing ischemic injury, mitochondrial metabolic damage and functional impairment in the skeletal muscle, and ultimately leading to immobility and mortality. While potential therapies have been mostly focussed on revascularization, none of the currently available pharmacological treatments are fully effective in PAD, often leading to amputations, particularly in chronic metabolic diseases. One major limitation of focussed angiogenesis and revascularization as a therapeutic strategy is a limited effect on metabolic restoration and muscle regeneration in the affected limb. Therefore, additional preclinical investigations are needed to discover novel treatment options …

Coordinated School Health 2022-23 Annual Report, Tennessee. Department Of Education.

Coordinated School Health

No abstract provided.

Student Preferences For Reference Services At A Remote Biological Station Library, Thom Gerrish, Scott Martin

Libraries Faculty and Staff Scholarship and Research

During the 2020 and 2021 summer semesters, the University of Michigan Biological Station (UMBS) transitioned to hybrid classes that were primarily distance learning with two-week inperson sections. The library offered both synchronous and asynchronous reference assistance over the summer term. An analysis showed that students favored using the UMBS LibGuide over synchronous virtual reference help via Zoom. Students further preferred face-to-face interactions over virtual formats, and their preference for LibGuide assistance may carry into the post- COVID-19 classroom. This finding suggests that students prioritize convenience and immediacy over personalized assistance in the Zoom platform. Thus, in providing reference assistance to …

Using Multiple Modes Of Learning In A High School Genetic Engineering Lesson, Hannah Stuart

Inclusive Strategies for Teaching Secondary Mathematics and Science

The goal of this article is to discuss a genetic engineering lesson presented to high school biology students to obtain mastery of the Biology Standard of Learning (BIO.5e) associated with genetic engineering products and practices. The lesson described in this article follows the 5E lesson plan model. Therefore, the article will describe the 5Es used in the creation and implementation of this lesson: engage, explore, explain, elaborate, and evaluate. This article focuses on the use of resources and activities that appeal to students with varying learning styles and looks at the effectiveness of individualized teacher-student attention during individual practice time. …

Microscope Investigations: An Inquiry-Based Cell Lab, Joshua Whittaker, R. Whitlow

Inclusive Strategies for Teaching Secondary Mathematics and Science

This lesson is a collaborative effort between a student teacher and a cooperating senior teacher to design and implement an engaging and exploratory microscope lab for 7th-grade students. The goal of this lesson is to cultivate excitement, interest, and exploration in students who are new to using microscopes, aligning with the Virginia 7th Grade Life Science Standards of Learning on cells and cell theory. This lab incorporates two microscope stations—one focusing on fundamental skills and the other on inquiry-based exploration. By employing the 5E model of instruction, this paper details how students progress through engagement, exploration, explanation, elaboration, and evaluation …

A Cellular Division Called Mitosis, Betsy Kent

Inclusive Strategies for Teaching Secondary Mathematics and Science

In biology, cellular reproduction is a fundamental process that ensures the growth and repair of living organisms. This article will detail the lesson that meets the needs of students with disabilities by applying several Universal Design for Learning (UDL) principles. The plan will be taught over a span of five days and focus on the fundamental process that allows living organisms to grow, repair damaged tissues, and reproduce. The students will have several hands-on activities that will enhance the lecture part of the lesson. Understanding cellular reproduction is crucial in comprehending the complexity and resilience of life itself.

Teaching Cells To Diverse Learners In The Modern Classroom, Avery Tully

Inclusive Strategies for Teaching Secondary Mathematics and Science

During this lesson, my main focus was to engage students in deeper learning about the importance of cells and all the aspects of structure, function, and history. My classroom is extremely diverse as I teach in Northern Virginia, a true melting pot. I have attached the population of minorities in Northern Virginia. This shows that over half of the population is minorities, well over the national average. You will also see the graph of ESOL homes in Northern Virginia. This graph shows that over 35% of homes in Northern Virginia speak another primary language other than English in their homes.

Cannabinoid Receptor Type 1 Regulates Sequential Stages Of Migration And Morphogenesis Of Neural Crest Cells And Derivatives In Chicken And Frog Embryos, Amira Mahomed, Daljit Girn, Afrin Pattani, Brian K. Wells, Chloe C. King, Sonya Patel, Harsimran Kaur, Christina M. Noravian, Jessica Sieminski, Chi Pham, Halley Dante, Maxellende Ezin, Tamira Elul

Biology Faculty Works

The main cannabinoid receptor CB1R first shows expression during early neurula stage in chicken (Gallus gallus) embryos, and at early tailbud stage in the frog (Xenopus laevis) embryos. This raises the question of whether CB1R regulates similar or distinct processes during the embryonic development of these two species. Here, we examined whether CB1R influences the migration and morphogenesis of neural crest cells and derivatives in both chicken and frog embryos. Early neurula stage chicken embryos were exposed to arachidonyl-2ʹ-chloroethylamide (ACEA; a CB1R agonist), N-(Piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251; a CB1R inverse agonist) or Blebbistatin (nonmuscle Myosin II inhibitor) in ovo and examined during …

Resting-State Functional Connectivity Correlates Of Attentional Bias In An Emotional Free Viewing Paradigm: An Eye-Tracking Investigation, Andrew Hauler

All NMU Master's Theses

Threat detection, the process of searching complex environments for harmful stimuli, represents a vastly important job that promotes the biological fitness of the organism. Decades of experimental evidence suggests individuals either diagnosed, or at risk for, affective disorders display altered patterns of attentional engagement (hypervigilance or maintenance) with external stimuli; referred to as attentional biases. To date, the extent to which underlying neural mechanisms drive attentional biases, both in affective disorders as well as unselected populations, remain to be resolved. Thus, using eye-tracking and a passive emotional free viewing task, this study set to clarify resting-state network contributions from three …

Ursinus Magazine, Fall 2023, Robyn E. Hannigan, Ed Moorhouse, Jennifer Meininger Wolfe, Ben Seal, William Mongan

Ursinus Magazine, 1995–Present

On the brain • Solving the problem • Brave in Season • Energy impact • Supporting recovery • Leading the conversation • Legacies of slavery • Bears on the Hill • At the Berman • Bear bites • Office space • The new view: How an Ursinus lab is threading machine learning and the human element • Lean on me: A system of student-led support • Pitch perfect • Welcome, new Bears! • Live from New York • Scenes on stage • On the fast track • Class notes • Marriages • In memoriam • Burning question: How can we …

Limited Accumulation And Persistence Of An Influenza A Virus In Tadpole Snails (Physa Spp.), Paul T. Oesterle, J. Jeffrey Root, Darcy S.O. Mora, Heather Schneider, Alan B. Franklin, Kathryn P. Huyvaert

United States Department of Agriculture Wildlife Services: Staff Publications

Waterfowl infected with avian influenza A viruses (IAVs) shed infectious virus into aquatic environments, providing a mechanism for transmission among waterfowl, while also exposing the entire aquatic ecosystem to the virus. Aquatic invertebrates such as freshwater snails are likely exposed to IAVs in the water column and sediment. Freshwater snails comprise a significant portion of some waterfowl species’ diets, so this trophic interaction may serve as a novel route of IAV transmission. In these experiments, tadpole snails (Physa spp.) were exposed to a low-pathogenicity IAV (H3N8) to determine whether snails can accumulate the virus and, if so, how long virus …

Regulation Of Tumor Apoptosis Of Poriae Cutis-Derived Lanostane Triterpenes By Akt/Pi3k And Mapk Signaling Pathways In Vitro, Shuai Yue, Xi Feng, Yousheng Cai, Salam A. Ibrahim, Ying Liu, Wen Huang

Faculty Research, Scholarly, and Creative Activity

Poria cocos is traditionally used as both food and medicine. Triterpenoids in Poria cocos have a wide range of pharmacological activities, such as diuretic, sedative and tonic properties. In this study, the anti-tumor activities of poricoic acid A (PAA) and poricoic acid B (PAB), purified by high-speed counter-current chromatography, as well as their mechanisms and signaling pathways, were investigated using a HepG2 cell model. After treatment with PAA and PAB on HepG2 cells, the apoptosis was obviously increased (p < 0.05), and the cell cycle arrested in the G2/M phase. Studies showed that PAA and PAB can also inhibit the occurrence and development of tumor cells by stimulating the generation of ROS in tumor cells and inhibiting tumor migration and invasion. Combined Polymerase Chain Reaction and computer simulation of molecular docking were employed to explore the mechanism of tumor proliferation inhibition by PAA and PAB. By interfering with phosphatidylinositol-3-kinase/protein kinase B, Mitogen-activated protein kinases and p53 signaling pathways; and further affecting the expression of downstream caspases; matrix metalloproteinase family, cyclin-dependent kinase -cyclin, Intercellular adhesion molecules-1, Vascular Cell Adhesion Molecule-1 and Cyclooxygenase -2, may be responsible for their anti-tumor activity. Overall, the results suggested that PAA and PAB induced apoptosis, halted the cell cycle, and inhibited tumor migration and invasion through multi-pathway interactions, which may serve as a potential therapeutic agent against cancer.

Genomic Approaches To Mitigating Genetic Diversity Loss In Declining Populations, Christen M. Bossu, Marina Rodriguez, Christine Rayne, Debra A. Chromczak, Philip G. Higgins, Lynne A. Trulio, Kristen C. Ruegg

Faculty Research, Scholarly, and Creative Activity

The accelerating pace of global biodiversity loss is exacerbated by habitat fragmentation and subsequent inbreeding in small populations. To address this problem, conservation practitioners often turn to assisted breeding programmes with the aim of enhancing genetic diversity in declining populations. Although genomic information is infrequently included in these efforts, it has the potential to significantly enhance the success of such programmes. In this study, we showcase the value of genomic approaches for increasing genetic diversity in assisted breeding efforts, specifically focusing on a highly inbred population of Western burrowing owls. To maximize genetic diversity in the resulting offspring, we begin …

Help-Seeking Behavior Among Resettled Refugee, Mahfoudha Sid’Elemine

Graduate Program in International Studies Theses & Dissertations

The refugee resettlement process is a huge part of refugees’ journeys in their new countries that has a lasting impact on their long-term integration. The literature highlights the importance of trust for successful refugee resettlement. This dissertation, however, challenges assumptions about the primary importance of “trust” and argues that the importance of trust is relative and can depend on the nature of the resettlement program and the perspective and background of refugees. In other words, there is not a one-size-fits-all of trust building in the context of resettlement. For certain resettlement programs trust building can be counterproductive when resources are …

Current Perspectives On Mass Spectrometry-Based Immunopeptidomics: The Computational Angle To Tumor Antigen Discovery, Bing Zhang, Michal Bassani-Sternberg

Faculty, Staff and Students Publications

Identification of tumor antigens presented by the human leucocyte antigen (HLA) molecules is essential for the design of effective and safe cancer immunotherapies that rely on T cell recognition and killing of tumor cells. Mass spectrometry (MS)-based immunopeptidomics enables high-throughput, direct identification of HLA-bound peptides from a variety of cell lines, tumor tissues, and healthy tissues. It involves immunoaffinity purification of HLA complexes followed by MS profiling of the extracted peptides using data-dependent acquisition, data-independent acquisition, or targeted approaches. By incorporating DNA, RNA, and ribosome sequencing data into immunopeptidomics data analysis, the proteogenomic approach provides a powerful means for identifying …

Mems 411: Dialysqueeze, Ranch Kimball, Maeve Lomax, Tyler Carlson

Mechanical Engineering Design Project Class

Design a device to reduce occupational fatigue injuries suffered by nurses who must manually clamp and unclamp tubing for dialysis machines. This device was designed for MEMS 411 Senior Design.

Mems 411: Medical Clamp, Edis Skampo, Rodery Gonzalez, Sokcheatra Samrith, Sean Lucas

Mechanical Engineering Design Project Class

The goal of the team was to design a plier that would be able to clamp down a medical clamp used for a dialysis machine. Ideally the plier should be able to clamp down 3 medical clamps on three separate dialysis tubes in less than 5 seconds, reduce the force required to clamp those clamps by 4 times, and weigh less than 113 grams. The product design was broken down into 3 separate concepts, with four options for each component. The most preferable option of each component was chosen and several risk factors were taken in consideration so that the …

Irish Studies News And Events 2023, Irish Studies

News, Magazines and Reports

Irish Studies at SHU has been growing! In our first newsletter, we are thrilled to share news about our students, faculty, events, courses, the Irish Studies minor, and research and scholarship projects happening in both in Fairfield and Dingle.

Library space houses the newly-donated Connecticut Irish-American Historical Society archives, called The Shanachie Room/Seomra an tSeanchaí, where our Irish language and Irish drama courses are meeting this semester.

Molecular And Biochemical Analysis Of Cytochrome P450 2c19 And 2d6, Reema Saleous

Theses

Pharmacogenomics (PGx) is a relatively new field of study. It links genetics to pharmacology since it deals with the influence of the genetic makeup of the individual on their ability to respond to specific medications. Some of the most important genes in this field, dubbed very important pharmacogenes (VIPs), belong to the cytochrome P450 (CYP) superfamily of drug metabolizing enzymes. The two members of this family that are the main focus of this thesis are CYP2C19 and CYP2D6. They play major roles in the metabolism of numerous medications, and it is therefore imperative that variations within those genes in various …

Fathoming Faith: A Philosophical Synthesis On Faith And Reason, Martin Wang

Loyola Papers

No abstract provided.

Contributors, Contributors Contributors

Loyola Papers

No abstract provided.

Recycling Of Electrode Materials From Spent Lithium-Ion Batteries To Develop Supercapacitor Devices, Yasmine Mesbah

Theses and Dissertations

Recently, there has been a rapid expansion in the utilization of lithium-ion batteries (LIBs) due to the propagation of portable electronic devices, electric vehicles, and renewable energy storage systems. Despite the plentiful advantages provided by LIBs, concerns have emerged regarding resource depletion and the environmental impact associated with their widespread use. The lack of essential resources such as lithium, cobalt, and nickel, along with the difficulties associated with managing battery waste, necessitates the development of sustainable strategies that encompass the entire lifecycle of LIBs. Recycling has arisen as a promising solution to combat these challenges and promote a more circular …

Design, Fabrication, And Evaluation Of 3-D-Printed Cystotomy Spoons As A Retrieval Method In Dogs, Davina C. D'Angelo, Sam E. Stephens, Morten O. Jensen, Lauren R. Thomas

Animal Science Faculty Publications and Presentations

OBJECTIVE

Current cystotomy methods often implement the use of off-label devices, resulting in urocystolith extraction difficulty and potentially leading to postoperative complications and discomfort for the patient. The objective of this study was to create 3 novel 3-D printed cystotomy spoons that offer a dedicated solution for removing urocystoliths from a patient's urinary bladder.

ANIMALS

Clinical use of the 3 novel 3-D printed cystotomy spoons were ultimately evaluated in 4 dogs and 1 cat that presented for urocystotlith removal at 3 different veterinary hospitals in northwest Arkansas.

METHODS

The novel cystotomy spoons were designed using SolidWorks, 3-D printed with a …