Digital Commons Network^™

Adaptive Design Of Mrna-Loaded Extracellular Vesicles For Targeted Immunotherapy Of Cancer, Shiyan Dong, Xuan Liu, Ye Bi, Yifan Wang, Abin Antony, Daeyong Lee, Kristin Huntoon, Seongdong Jeong, Yifan Ma, Xuefeng Li, Weiye Deng, Benjamin R Schrank, Adam J Grippin, Jonghoon Ha, Minjeong Kang, Mengyu Chang, Yarong Zhao, Rongze Sun, Xiangshi Sun, Jie Yang, Jiayi Chen, Sarah K Tang, L James Lee, Andrew S Lee, Lirong Teng, Shengnian Wang, Lesheng Teng, Betty Y S Kim, Zhaogang Yang, Wen Jiang

Faculty, Staff and Student Publications

The recent success of mRNA therapeutics against pathogenic infections has increased interest in their use for other human diseases including cancer. However, the precise delivery of the genetic cargo to cells and tissues of interest remains challenging. Here, we show an adaptive strategy that enables the docking of different targeting ligands onto the surface of mRNA-loaded small extracellular vesicles (sEVs). This is achieved by using a microfluidic electroporation approach in which a combination of nano- and milli-second pulses produces large amounts of IFN-γ mRNA-loaded sEVs with CD64 overexpressed on their surface. The CD64 molecule serves as an adaptor to dock …

2023-10-19 Institutional Animal Care And Use Committee Meeting Minutes, Morehead State University. Faculty Senate., Institutional Animal Care And Use Committee.

Faculty Senate Records

The meeting minutes of the Institutional Animal Care and Use Committee for October 19, 2023.

A Thesis On Phosphatidylserine As A Key Signaling Moiety During Adipocyte Differentiation, Brady Jeffrey Barron

Arts & Sciences Graduate Student Theses and Dissertations

The adipose tissue is composed of specialized energy storage cells called adipocytes. In white adipose tissue, when committed precursor cells (preadipocytes), receive specific cues, the preadipocytes differentiate into mature adipocytes via an adipogenic gene program promoted by transcription factors such as PPAR. Phosphatidylserine (PS) is a phospholipid asymmetrically maintained on the inner leaflet of the plasma membrane in healthy cells. PS flipping to the outer leaflet is a well-known hallmark of cell death, such as apoptosis. Here, while exploring the interplay between PS exposure and adipose tissue homeostasis, we made a surprising discovery in that non apoptotic PS exposure occurs …

Water Beetles (Coleoptera) Associated With Afrotemperate Forest Patches In The Garden Route National Park, South Africa, Matthew S. Bird, David T. Bilton, Musa C. Mlambo, Renzo Perissinotto

School of Biological and Marine Sciences

Southern Afrotemperate Forest is concentrated in the southern Cape region of South Africa and whilst it is relatively well known botanically, the fauna, specifically the aquatic invertebrate fauna, is poorly documented. The majority of remaining intact forest habitat is contained within the Garden Route National Park (GRNP), which straddles the provincial boundary between the Western and Eastern Cape. This study undertakes a survey of the water beetle fauna inhabiting the GRNP. The aquatic ecosystems within temperate forests of the region are poorly researched from an ecological and biodiversity perspective, despite being known to harbour endemic invertebrate elements. We collected water …

Vista: October 19, 2023, University Of San Diego

USD Vista (1965-)

No abstract provided.

Kenyon Collegian - October 19, 2023

The Kenyon Collegian

No abstract provided.

The Urgent Need For Preparing Anti-Oppressive Citizens In Elementary Social Studies: A Conceptual And Pragmatic Framework For Educators, Erin Piedmont

Teaching Social Studies in the Peach State

No abstract provided.

Commonwealth Times 2023-10-18

Commonwealth Times, 1969-

No abstract provided.

Bioinspired Materials For Underwater Adhesion With Pathways To Switchability, Chanhong Lee, Huiqi Shi, Jiyoung Jung, Bowen Zheng, Kan Wang, Ravi Tutika, Rong Long, Bruce Lee, Grace X. Gu, Michael D. Bartlett

Michigan Tech Publications

Strong adherence to underwater or wet surfaces for applications like tissue adhesion and underwater robotics is a significant challenge. This is especially apparent when switchable adhesion is required that demands rapid attachment, high adhesive capacity, and easy release. Nature displays a spectrum of permanent to reversible attachment from organisms ranging from the mussel to the octopus, providing inspiration for underwater adhesion design that has yet to be fully leveraged in synthetic systems. Here, we review the challenges and opportunities for creating underwater adhesives with a pathway to switchability. We discuss key material, geometric, modeling, and design tools necessary to achieve …

Mitochondrial Transplantation And Transfer: The Promising Method For Diseases, Gökhan Burçi̇n Kubat

Turkish Journal of Biology

Mitochondria are organelles that serve as the powerhouses for cellular bioenergetics in eukaryotic cells. It is responsible for mitochondrial adenosine triphosphate (ATP) generation, cell signaling and activity, calcium balance, cell survival, proliferation, apoptosis, and autophagy. Mitochondrial transplantation is a promising disease therapy that involves the recovery of mitochondrial dysfunction using isolated functioning mitochondria. The objective of the present article is to provide current knowledge on natural mitochondrial transfer processes, in vitro and in vivo applications of mitochondrial transplantation, clinical trials, and challenges associated with mitochondrial transplantation.

Investigating The Roles Of Active Site Residues In Mycobacterium Tuberculosis Indole-3-Glycerol Phosphate Synthase, A Potential Target For Antitubercular Agents, David W. Konas, Sarah Cho, Oshane D. Thomas, Maryum M. Bhatti, Katherine Leon Hernandez, Cinthya Moran, Hedda Booter, Thomas Candela, Joseph Lacap, Paige Mcfadden, Savannah Van Den Berg, Alyssa M. Welter, Ashley Peralta, Cheryl A. Janson, Jaclyn Catalano, Nina M. Goodey

Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works

Mycobacterium tuberculosis drug resistance is emerging and new drug targets are needed. Tryptophan biosynthesis is necessary for M. tuberculosis replication and virulence. Indole-3-glycerol phosphate synthase (IGPS) catalyzes a step in M. tuberculosis tryptophan biosynthesis and has been suggested as a potential anti-infective target, but our understanding of this enzyme is limited. To aid in inhibitor design and gain a greater mechanistic picture of this enzyme, there is a need to understand the roles of active site amino acids in ligand binding and catalysis. In this work, we explored the roles of conserved active site amino acids Glu57, Lys59, Lys119, Glu168, …

Caracterización De Poblaciones De Especies Endémicas En Bosque Tropical Seco Semideciduo. Parque Nacional Machalilla, Manabí, Ecuador, Joselyn K. Castro, Jimmy J. Cevallos Zambrano, Francisco X. Pico Franco, Juan C. Palacios Peñafiel, Esteban Chirino Miranda

Neotropical Biodiversity

No abstract provided.

Criminalization Of Environmental Harms In Bangladesh And The United States, Md Lokman Hussain

Theses and Dissertations

This study explores and compares the nature, features, and measures of criminalization of environmental harms in Bangladesh and United States. The exploration is based on the descriptive and analytical comparison of the legal frameworks addressing the environmental harms in these two countries. To explore how are environmental harms criminalized in Bangladesh and United States, following aspects were described and compared: the criminalizing power of the state, criminalization of environmental harms regarding air pollution, water pollution, protection of biodiversity, waste management, and use of pesticide, theoretical frameworks in explaining criminalization, role of constitution and international law.

Polar Oceans And Sea Ice In A Changing Climate, Megan D. Willis, Delphine Lannuzel, Brent Else, Hélène Angot, Karley Campbell, Odile Crabeck, Bruno Delille, Hakase Hayashida, Martine Lizotte, Brice Loose, Klaus M. Meiners, Lisa Miller, Sebastien Moreau, Daiki Nomura, John Prytherch, Julia Schmale, Nadja Steiner, Letizia Tedesco, Jennie Thomas

Graduate School of Oceanography Faculty Publications

Polar oceans and sea ice cover 15% of the Earth’s ocean surface, and the environment is changing rapidly at both poles. Improving knowledge on the interactions between the atmospheric and oceanic realms in the polar regions, a Surface Ocean-Lower Atmosphere Study (SOLAS) project key focus, is essential to understanding the Earth system in the context of climate change. However, our ability to monitor the pace and magnitude of changes in the polar regions and evaluate their impacts for the rest of the globe is limited by both remoteness and sea-ice coverage. Sea ice not only supports biological activity and mediates …

The Lumberjack, October 18, 2023

Cal Poly Humboldt's Student Newspaper

The student newspaper of Humboldt State University.

Androgen Receptor Contributes To Repairing Dna Damage Induced By Inflammation And Oxidative Stress In Prostate Cancer, Bi̇lge Debeleç Bütüner, Nurşah Ertunç Hasbal, Eli̇f İşel, Dirk Roggenbuck, Kemal Sami̇ Korkmaz

Turkish Journal of Biology

Background: Androgen deprivation therapy remains the first-line therapy option for prostate cancer, mostly resulting in the transition of the disease to a castration-resistant state. The lack of androgen signaling during therapy affects various cellular processes, which sometimes paradoxically contributes to cancer progression. As androgen receptor (AR) signaling is known to contribute to oxidative stress regulation, loss of AR may also affect DNA damage level and the response mechanism in oxidant and inflammatory conditions of the prostate tumor microenvironment. Therefore, this study aimed to investigate the role of AR and AR-regulated tumor suppressor NKX3.1 upon oxidative stress-induced DNA damage response (DDR) …

Improving Human-Automation Collaboration In Motion Planning, Torin J. Adamson

Computer Science ETDs

Human-automation collaboration is becoming a part of everyday life as AI helps us drive, make decisions, and solve a variety of other tasks. However, safe and effective collaboration systems depend on factors in trust, communication, and more. Existing studies to explore these are typically carried out in laboratory settings, providing robust data under tight environmental control. However, human behavior evolves over time, driven by external factors that cannot be fully captured in single participation sessions. These factors form the "human context", contextualizing the behavioral data for a more complete understanding. In this thesis, video game adaptations upon conventional subject studies …

Advances And Challenges In Bioinformatics And Biomedical Engineering: Iwbbio 2020, Olga Valenzuela, Mario Cannataro, Irena Rusur, Jianxin Wang, Zhongming Zhao, Ignacio Rojas

Faculty, Staff and Student Publications

This Supplement issue, presents five research articles which are distributed, mainly due to the subject they address, from the 8th International Work-Conference on Bioinformatics and Biomedical Engineering (IWBBIO 2020), which was held on line, during September, 30th-2nd October, 2020. These contributions have been chosen because of their quality and the importance of their findings. Those contributions were then invited to participate in this supplement for the following journals of BMC: BMC Bioinformatics and BMC Genomics. In the present Editorial in BMC journal, we summarize the contributions that provide a clear overview of the thematic areas covered by the IWBBIO conference, …

Cover And Contents

Turkish Journal of Biology

No abstract provided.

Gooseberry Anthocyanins Alleviate Insulin Resistance By Regulating Ceramide Metabolism In High Fat Diet Mice, Xian Tang, Jun Gao, Jinpeng Huang, Chenjuan Zhang, Hongwei Liu, Jie Wei

Turkish Journal of Biology

Background/aim: Obesity is the fifth largest risk factor of death in the world. The ceramide produced by obesity is closely related to insulin resistance (IR) caused by obesity. At present, the commercially available weight loss products have large side effects and limited therapeutic effects. Therefore, it is particularly important to find effective natural nontoxic products to treat obesity and explore its possible pathways and mechanisms. Materials and methods: In this paper, a high-fat diet (HFD) mice model was established by intragastric administration of high-fat emulsion to investigate the intervention effect of Gooseberry anthocyanins (GA) on IR in HFD mice. We …

Exploring Breast Cancer Systemic Drug Therapy Patterns In Real-World Data, Julia O'Rourke, Jeff Warnick, John Doole, Luc De Keyser, Zuzanna Drebert, Olivia Wan, Courtney N Thompson, Jack W. London, Karen Fairchild, Matvey B. Palchuk

Kimmel Cancer Center Faculty Papers

PURPOSE: To explore medications and their administration patterns in real-world patients with breast cancer.

METHODS: A retrospective study was performed using TriNetX, a federated network of deidentified, Health Insurance Portability and Accountability Act-compliant data from 21 health care organizations across North America. Patients diagnosed with breast cancer between January 1, 2013, and May 31, 2022, were included. We investigated a rule-based and unsupervised learning algorithm to extract medications and their administration patterns. To group similar administration patterns, we used three features in k-means clustering: total number of administrations, median number of days between administrations, and standard deviation of the days …

2023-10-18 Mariners Miss Playoffs Again, Fans Still Hopeful For Future, Marisa Sexsmith

The Spectator Online

No abstract provided.

Ribosomal Protein L8 Regulates The Expression And Splicing Pattern Of Genes Associated With Cancer-Related Pathways, Leilei Xu, Gui Yang, Bin Song, Dong Chen, Akbar. Yunus, Jiangtao Chen, Xiaogang Yang, Zheng Tian

Turkish Journal of Biology

Background/aim: Ribosomal proteins have been shown to perform unique extraribosomal functions in cell apoptosis and other biological processes. Ribosomal protein L8 (RPL8) not only has important nonribosomal regulatory functions but also participates in the oncogenesis and development of tumors. However, the specific biological functions and pathways involved in this process are still unknown. Materials and methods: RPL8 was overexpressed (RPL8-OE) in HeLa cells. MTT assay and flow cytometry were used to detect cell proliferation and apoptosis, respectively. Transcriptome sequencing was performed to analyze the differentially expressed genes (DEGs) and regulated alternative splicing events (RASEs) by RPL8-OE, both of which were …

Braf D594a Mutation Defines A Unique Biological And Immuno-Modulatory Subgroup Associated With Functional Cd8+ T Cell Infiltration In Colorectal Cancer, Wenjing Li, Chenyi Zhao, Wenhui Li, Yang Gong, Kaili Ma, Yujie Lu, Xiaowei Liu, Lianjun Zhang, Feng Guo

Faculty, Staff and Student Publications

BACKGROUND: BRAF non-V600 mutation occupies a relatively small but critical subset in colorectal cancer (CRC). However, little is known about the biological functions and impacts of BRAF class III mutation in CRC. Here, we aim to explore how D594A mutation impacts on biological behaviors and immune related signatures in murine CRC cells.

METHODS: BRAF V600E (class I), G469V (class II) and D594A (class III) mutant cell lines were established based on MC38 cells. The biological behaviors of cells were evaluated in respect of cell growth, cell proliferation, cell apoptosis, cell migration and invasion by the methods of colony-forming assay, CCK-8 …

Editorial: Debates In Cardiovascular Pharmacology And Drug Discovery: 2022, Keman Xu, Fatma Saaoud, Ying Shao, Yifan Lu, Xiaohua Jiang, Sheng Wu, Jianxin Sun, Filipe Fernades Conti, Laisel Martinez, Roberto Vazquez-Padron, Hong Wang, Xiaofeng Yang

Center for Translational Medicine Faculty Papers

No abstract provided.

Minutes From October 18 2023, University Senate

University Senate Minutes

Minutes from October 18 2023 University Senate Meeting

Genetic Analysis Of Basal Stalk Rot Resistance Introgressed From Wild Helianthus Petiolaris Into Cultivated Sunflower (Helianthus Annuus L.) Using An Advanced Backcross Population, Zahirul I. Talukder, William Underwood, Christopher G. Misar, Xuehui Li, Gerald J. Seiler, Xiwen Cai, Lili Qi

Department of Agronomy and Horticulture: Faculty Publications

Introduction: Sclerotinia sclerotiorum is a serious pathogen causing severe basal stalk rot (BSR) disease on cultivated sunflower (Helianthus annuus L.) that leads to significant yield losses due to insufficient resistance. The wild annual sunflower species H. petiolaris, commonly known as prairie sunflower is known for its resistance against this pathogen. Sunflower resistance to BSR is quantitative and determined by many genes with small effects on the resistance phenotype. The objective of this study was to identify loci governing BSR resistance derived from H. petiolaris using a quantitative trait loci (QTL) mapping approach.

Methods: BSR …

The Association Of Research Quantitative Measures With Faculty Ranks Of Australian And New Zealand Dental Schools, Ayesha Fahim, Sadia Shakeel, Farhan Saleem, Ijaz Ur Rehman, Kashif Siddique, Habib Ahmad Qureshi, Muhammad Sohail Zafar

Research outputs 2022 to 2026

Introduction: The scholarly productivity of a faculty member can be measured through several indicators including annual appraisals, feedback, and the number of publications per year. The present study aims to assess the association of quantitative research measures and academic ranks in Australian and New Zealand dental schools. Methods: It was an analytical observational cross-sectional study. Full-time faculty members working in Australia and New Zealand's dental schools were discovered on official websites. Various bibliometric parameters including h-index, total number of citations, total number of publications, and maximum number of sources of a single publication were analyzed. Spearman rank correlation was used …

Winonan, Winona State University

The Winonan - 2020s

This issue of the Winonan, the Winona State University student newspaper, was published on October 18, 2023.

Depth And Frequency Dependence Of Geoacoustic Properties On The New England Mud Patch From Reflection Coefficient Inversion, Jiang Yong-Min, Charles W. Holland, Stan E. Dosso, Jan Dettmer

Electrical and Computer Engineering Faculty Publications and Presentations

Muddy sediments cover significant portions of continental shelves, but their physical properties remain poorly understood compared to sandy sediments. This paper presents a generally applicable model for sediment-column structure and variability on the New England Mud Patch (NEMP), based on trans-dimensional Bayesian inversion of wide-angle, broadband reflection-coefficient data in this work and in two previously published reflection-coefficient inversions at different sites on the NEMP. The data considered here include higher frequencies and larger bandwidth and cover lower reflection grazing angles than the previous studies, hence, resulting in geoacoustic profiles with significantly better structural resolution and smaller uncertainties. The general sediment-column …