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Male Infertility Is Significantly Associated With Multiple Deletions In An 8.7-Kb Segment Of Sperm Mtdna In Pakistan, Irfan Afzal Mughal, Asma Irfan, Sarwat Jahan, Abdul Hameed
Male Infertility Is Significantly Associated With Multiple Deletions In An 8.7-Kb Segment Of Sperm Mtdna In Pakistan, Irfan Afzal Mughal, Asma Irfan, Sarwat Jahan, Abdul Hameed
Turkish Journal of Medical Sciences
Background/aim: This study aimed to find a link between sperm mitochondrial DNA mutations and male infertility in Pakistan. Materials and methods: DNA from semen samples was extracted and amplified by PCR using 7.8-kb deletion-specific primers. The PCR products were separated on agarose gel, visualized under UV-illumination, and then photographed. The results were genotyped and the data were analyzed using SPSS. Results: Deletion analysis of the 8.7-kb fragment by long PCR revealed multiple deletions. The frequency of deletion was much higher in infertile groups as compared to the control group. Further, on comparison between different subtypes of infertile groups, the deletions …