Digital Commons Network^™

Bim-Safety Integration In The Nigerian Construction Industry Using The Unified Theory Of Acceptance And Use Of Technology (Utaut) Model, Ranti T. Adebiyi, Ganiyu Amuda-Yusuf, Olubola Babalola, Nurudeen A. Bello, Aminat D. Amuda, Kaosarat A. Olowookere

Engineering and Technology Journal

Health and Safety (H&S) challenges are a significant concern in the Nigerian construction industry, largely due to the lack of a reliable national accident database and the persistence of a fatalistic safety culture. Building Information Modelling (BIM) is a transformative digital framework that enables visual hazard identification and proactive risk simulation; however, its use in site safety management remains limited and inconsistent. This study investigates the behavioural, social, and structural determinants influencing the adoption of BIM-based H&S practices among construction professionals, utilising the Unified Theory of Acceptance and Use of Technology (UTAUT). A hypothetical multi-level design was used to model …

Token-Efficient Verification For Self-Improving Root-Cause-Analysis Agents, Necati Cehreli

Defensive Publications Series

Proposed herein is a system that chooses how much testing is needed for an improvement to an artificial intelligence (AI) root cause analysis (RCA) agent before the system trusts the improvement. Small, local changes can receive quick replay first, while broad or risky changes require a full rerun or multi-case batch testing. One result of the proposed approach is a safer and less costly self-improvement loop through which a system can spend additional testing effort when the change warrants such testing.

Deterministic, Model-Independent Grounding-Integrity Mechanisms For Retrieval-Augmented And Agentic Language-Model Systems, Roberto A. Wenzel

Defensive Publications Series

Revision 1.2 of a defensive publication disclosing deterministic, model-independent grounding-integrity mechanisms for retrieval-augmented and agentic LLM systems. The mechanisms make citation resolvability an invariant of every output mode routed through a cross-tool evidence registry — detect → bounce → strip with compound-citation surgery, an ordering rule, and a strip-event notice — and make degradations of grounding (a removed citation, a deleted source, an unverified item, a suspect "not found") visible to the reviewer rather than silent. Revision 1.2 adds Technique G (decompose-and-verify: per-unit grounding, byte-identical scoped regeneration, de-correlated verification, citation tombstones, honest terminal states), a precise trust model and guarantee …

Evidence-Partitioned Autonomy Authorization For Llm Agents: Single-Use Capability Tokens, A Selection-Bias Firewall, And Pre-Outcome Committed Audit Selection In A Cross-Model Execution-Authority Control Loop, Jason Edward Plumb

Defensive Publications Series

Large-language-model agents are routinely granted execution authority they have not earned: per-call permission prompts do not scale, allowlists do not adapt, and commercial “trust scores” are heuristic. This disclosure describes a deterministic, fail-closed execution-authority control loop in which model outputs are never authority. One model may only propose typed, exactly-argued actions; a second model may only request execution of an unmodified proposal; a host-owned controller authorizes each operation immediately before it executes; and a gateway redeeming single-use capability tokens is the only path to side effects. Four mechanisms are disclosed in enabling detail: (1) an autonomy-evidence store partitioned by a …

Macro-Scale Ultra-Vacuum Containment Vaults (Uvcv) And Detached Robotic Logistics Logs For The Absolute Elimination Of Interfacial Oxidative Degradation, Christopher L. Eckes

Defensive Publications Series

This specification formalizes the macroscopic architectural layout, atmospheric seal metrics, and automated robot-to-machine routing protocols required to establish an external environmental guardrail for high-throughput Elemental Transmutation Replicator (ETR) industrial nodes. Prior art (Paper 128) details the micro-scale electro-hydrodynamic lamination of edible protective polymer skins over hyper-accelerated macro-nutrient streams. However, mechanical nozzle failures or unexpected microfluidic backpressure cascades introduce an operational vulnerability: transient exposure to ambient atmospheric oxygen (O₂) and water vapor (H₂O) can cause instant material degradation and structural collapse during high-velocity extrusion passes.

This disclosure resolves this structural vulnerability by placing the entire replication assembly inside a sealed, structural …

System Architecture For Ai-Synchronized Phononic Suppression Matrices And Solitonic Disruption Of The Exothermic Bonding Barrier In Atomic Layer Deposition Cores, Christopher L. Eckes

Defensive Publications Series

This specification formalizes the architectural integration, hardware layouts, and predictive real-time control algorithms required to transition the Phase 1 Ultra-Vacuum Synthesis Bed of the Elemental Transmutation Replicator (ETR) framework (Prior Art: TDC #11772) from an assembly-throttled safety regime to a hyper-accelerated, continuous-flow manufacturing matrix. Prior art (CART-CE #12157) establishes that instantaneous molecular synthesis yields extreme, localized exothermic enthalpy spikes (ΔH°f) that overwhelm the passive thermal conductivity (κ) of Silicon Carbide (SiC) dampening hulls, triggering automated laser throttling and dropping system velocity to protect structural interfaces.

This disclosure resolves this structural material bottleneck by implementing an AI-Governed Active Phononic Dampening Field. …

System And Method For Monitoring Client Logging Reliability, Quentin Fiard, Dakshi Goyal, David Phillips

Defensive Publications Series

A monitoring system and method for benchmarking client logging reliability across multiple logging systems. The monitoring system includes a client-side component (prober) and a serverside component (server-side pipeline). The prober identifies scenarios, initializes and increments sequence numbers scoped to stream identifier and scenario combinations, generates probes containing sequence numbers and stream identifiers, and dispatches probes through multiple logging systems. The server-side pipeline aggregates probes over a rolling window by event creation date, joins probe records across logging systems to construct a ground truth, detects gaps in sequence numbers, and calculates reliability metrics for each logging system.

Hybrid Graph-Neural Net And Resource Description Framework-Star (Rdf-Star) Semantic Signal Ranking With Sparse-Semantics Recovery And Provenance-Aware Output, Vladimir Mikhailov, Raja Soundaramourty

Defensive Publications Series

A ranking service receives sparse request input and ranks candidates using a hybrid graph-neural and semantic-evidence architecture. The service computes graph-derived relevance using a Graph Neural Network (GNN), retrieves Resource Description Framework-star (RDF-star) statement-level semantic-relationship evidence, and computes a semantic score from the retrieved evidence. When direct semantic evidence is unavailable, bounded runtime fallback uses attenuated one-hop neighbor evidence. The service blends the graph-derived relevance and semantic score to produce ranked results with score decomposition and provenance indicators. The architecture improves semantic-context coverage and explainability for sparse enterprise graphs while preserving baseline neural-ranking behavior.

Endpoint Encryption And Ai-Agent Access Control For Preventing Autonomous Agent Access To Sensitive Files While Preserving Human User Access, Hp Inc

Defensive Publications Series

This idea introduces a method and system for preventing generative AI tools, agentic AI processes, and autonomous software agents from accessing sensitive files on an endpoint device while still allowing authorized human users to access those files through controlled decryption mechanisms. The disclosure extends endpoint file tagging to classify files as Personal/Personally Identifiable Information (PII), Enterprise Confidential, Trusted, or Untrusted, and applies access enforcement at the point of file access. Sensitive files are protected using asymmetric encryption, with private keys held within a Trusted Platform Module (TPM), secure enclave, microvisor, hypervisor, or equivalent protected control plane. Human users can access …

Techniques For Handling Unstable Agents In Agent-Based Networks, Ashwini S Kulkarni, Sushmitha Vatsal, Priyanka Bansal, Vivek Kumar Singh, Ghulam Ghaus Arshi

Defensive Publications Series

Proposed herein are a system and techniques that facilitate stability-aware metadata synchronization in agent-based networks. The system includes a closed-loop adaptive stability orchestration layer that utilizes a time-sensitive exponential-decay mechanism and historical recovery telemetry to dynamically tune suppression and reuse thresholds, thereby reducing control-plane churn and cascading failures. Rather than relying on binary health checks, the system uses a time-weighted stability score to support granular, policy-driven agent lifecycle management.

Method And System For Adaptive Multi-Channel Employee Performance Assessment With Dynamic Data Source Weighting And Entropy-Based Filtering, Vladimir Gorobtsov

Defensive Publications Series

The present disclosure describes a method and system for comprehensive employee performance assessment using adaptive multi-channel data integration. The system collects data from at least three independent sources (psychometric tests, physical activity monitoring, operational workload metrics, communication activity, and behavioral logs). It employs Shannon entropy-based filtering to exclude noisy sources, Tukey's interquartile range method for outlier removal, and sliding-window normalization. Dynamic weights are calculated daily based on statistical dependence between each data source and actual employee productivity. An integrated performance index is computed with seasonal adjustment. The system is scalable, computationally efficient, and reduces processing load by up to 40%. …

A Process For The Preparation Of Gepotidacin Mesylate And Its Intermediates, Lee Fine Chem Private Limited

Defensive Publications Series

The present disclosure relates to 4-[[(3,4-dihydro-2H-pyrano[2,3-c]pyridin-6-yl)methyl]amino]-1-acetylpiperidine, is represented by following structural formula (5) and its process for the preparation thereof.

The present disclosure further relates to use of 4-[[(3,4-dihydro-2H-pyrano[2,3-c]pyridin-6-yl)methyl]amino]-1-acetylpiperidine of Formula (5) for the preparation of Gepotidacin mesylate is represented by following structural formula (1).

Dual-Polymer Concrete With Recycled Pet And Hdpe Aggregates: Mechanical And Durability Performance Assessment, Omar Abd El Mageed Hammouda

Theses and Dissertations

This study investigates the fresh, mechanical, durability, thermal, performance as well as preliminary financial aspects of dual-polymer concrete incorporating recycled polyethylene terephthalate (PET) as a partial replacement for fine aggregate and recycled high-density polyethylene (HDPE) as a partial replacement for coarse aggregate, with silica fume as a supplementary cementitious material held constant at 10% by weight of cement. The study responds to the convergence of two pressing challenges in the Egyptian context: the scale of plastic waste mismanagement, which results in widespread informal open burning and environmental contamination, and the high operational energy demand of residential buildings in Egypt's hot …

An In-Depth Study Of Fruit Preservation: Effect Of Calcium Chloride And Methyl Jasmonate Applications On Quality And Biochemical Properties Of Cherry Fruits During Cold Storage, Muhammet Ali̇ Gündeşli̇

Turkish Journal of Agriculture and Forestry

Sweet cherry (Prunus avium L.) is a highly perishable fruit that undergoes rapid quality deterioration during postharvest storage. This study evaluated the effects of postharvest applications of Methyl Jasmonate (MeJA, 1 mM) and Calcium Chloride (CaCl₂, 2%), applied individually and in combination, on maintaining the quality and biochemical properties of cherries during 21 days of cold storage at 0 ± 1 °C. Key parameters including weight loss, decay rate, soluble solids content (SSC), pH, titratable acidity, respiration rate, phenolic profile, and organic acid composition were monitored. The combined MeJA + CaCl₂ treatment was consistently more effective than individual applications in …

Sewage Sludge Legislation In Central Europe: Contamination Concerns And Cross-Border Comparisons, Valerie Vranová, Aleš Kučera, Vlastimil Skoták, Erika Fialová, Rahul Datta

Turkish Journal of Agriculture and Forestry

Sewage sludge management represents a major challenge in Europe, carrying significant environmental and legal implications that require strong regulatory enforcement. Here, we explore the legislative measures, technological developments, and environmental impacts of sewage sludge management in Central Europe (Czechia, Slovakia, Germany, Austria, and Poland). Sludge management is regulated at the EU level through a series of directives (e.g., Council Directive 86/278/EEC, which forms the basis of national regulatory frameworks). However, national adaptations vary considerably: Croatia and Slovenia have invested in sludge phosphorus-recovery systems, while Poland and Slovakia have focused on the agricultural use of sludge as a fertilizer. Several countries, …

Demographic And Clinical Characteristics Of Frequent Users Of Emergency Medical Services In Türkiye ​, Burak Bekgöz, İshak Şan

Turkish Journal of Medical Sciences

Background/aim: Overuse of emergency medical services (EMS), particularly unnecessary ambulance requests, places considerable strain on limited healthcare resources and can delay responses to urgent cases. The present study analyzes the demographic characteristics, diagnoses, urgency, and hospital transfer status of frequent users of EMS in Türkiye.

Materials and methods: Included in the study were 41,113 patients who requested EMS four or more times within a year (defined as frequent users for the purpose of the study), excluding interhospital transfers. Data were accessed from the Emergency Medical Services Automation System (ASOS) database, which contains details of patient demographics, diagnoses, triage codes, reasons …

Male Osteoporosis: Pathophysiology, Risk Assessment, And Evidence-Based Management, Seli̇n Teki̇n, Alper Gürlek

Turkish Journal of Medical Sciences

Osteoporosis in men is a common yet underrecognized condition associated with substantial fracture-related morbidity and excess mortality. Although men generally attain higher peak bone mass than women, age-related changes in bone microarchitecture, progressive cortical bone loss, declining sex steroid bioavailability, and a high prevalence of secondary causes contribute to a significant lifetime fracture risk. Importantly, a large proportion of fragility fractures in men occur at bone mineral density (BMD) values above the osteoporotic threshold, highlighting the limitations of BMD-based diagnosis alone. This review provides a comprehensive overview of the epidemiology, pathophysiology, and clinical characteristics of osteoporosis in men, with particular …

Hearing Loss In Behçet’S Syndrome: Prevalence And Associated Factors In A Nationwide Cross-Sectional Analysis ​, Alper Doğanci, Serdar Can Güven, Nuray Yilmaz Çakmak, Nurhan Güven, Sali̇h Başer, Nesli̇han Öztürk, Şuayi̇p Bi̇ri̇nci̇, Nai̇m Ata

Turkish Journal of Medical Sciences

Background/aim: The aim of this study was to investigate the prevalence of hearing loss in Behçet’s syndrome and its associations with other disease manifestations, comorbidities, and treatment agents. To the best of our knowledge, this is the most comprehensive study to date addressing this issue.

Materials and methods: The study was designed as a cross-sectional, nationwide study. The Turkish Ministry of Health National Electronic Database (e-Nabız) was used under the supervision of the Ministry of Health to extract patient data. A nationwide cohort was established from patients who had been assigned an ICD-10 code for BS (M35.2) on at least …

A Comparative Analysis Of Clinical Quality And Readability Of Neuropathic Pain Patient Information Leaflets: Türkiye Versus The United States, Gonul Sari, Argun Pire

Turkish Journal of Medical Sciences

Background/aim: Neuropathic pain management requires high patient adherence and complex dose-titration regimens, making the readability of patient information leaflets (PILs) a critical patient safety factor. This study aimed to compare the clinical content quality and linguistic readability of PILs for neuropathic pain medications between Türkiye (TİTCK) and the United States (DailyMed).

Materials and methods: A total of 11 pairs of innovator medications were analyzed. Clinical content was assessed using the 15-item Keystone Clinical Content Checklist (KCCC). Readability was assessed using the Ateşman index for Turkish texts and the Flesch Reading Ease Score (FRES) for English texts. Information quality and reliability …

Evaluation Of Acne Vulgaris Patient Care In Outpatient Clinics: Guideline Adherence And Patient Education ​, Mefküre Durmuş, Betül Sena Çavdar, İbrahi̇m Etem Arica, Sena Ustaömer, Sena Güzel Karahan, Seçi̇l Çolak, Selcen Şenol

Turkish Journal of Medical Sciences

Background/aim: Acne vulgaris, affecting over 85% of adolescents, is the most prevalent chronic inflammatory skin disorder. Its management is severity-based, with specific drug selections and combinations according to guidelines. However, adherence to these guidelines and the adequacy of patient counseling remain uncertain in real-world practice. The aim in this study was to evaluate prescribing patterns in acne management, assess compliance with guidelines, and promote awareness among healthcare professionals.

Materials and methods: A prospective observational study was conducted in the dermatology outpatient clinic of a tertiary hospital in Türkiye between February and May 2025. Demographic and clinical data were collected, and …

Measurement Of Resection Volume Using Computed Tomography 3d Modeling In Lung Cancer Patients And Survival Analysis ​, Aykut Kankoç, Muhammet Tarik Aslan, Muhammet Sayan, Ali̇ Çeli̇k, Cüneyt İsmai̇l Kurul, Abdullah İrfan Taştepe

Turkish Journal of Medical Sciences

Background/aim: The prognostic significance of the volumetric extent of lung resection in nonsmall cell lung cancer remains uncertain. Three-dimensional computed tomography allows quantitative assessment of resected lung parenchyma, but the relationship between resection volume ratios and oncologic outcomes has not been clearly established. The aim in this study was to evaluate the prognostic value of three-dimensional computed tomography-derived resection volume ratios for disease-free survival after surgery for nonsmall cell lung cancer.

Materials and methods: This single-center retrospective study included 141 patients who underwent anatomical resection with systematic mediastinal lymph node dissection between 2010 and 2021. Resection volume, ipsilateral lung volume, …

Perioperative Effects Of The Quadratus Lumborum Block In Percutaneous Biliary Drainage: A Prospective Randomized Controlled Study ​, Seyyi̇d Furkan Kina, Can Ozan Yazar, Cem Koray Çataroğlu, Erdi̇ Tangobay, Sadik Ahmet Uyanik, Savaş Altinsoy, Reyhan Polat, Jüli̇de Ergi̇l

Turkish Journal of Medical Sciences

Background/aim: The aim of this study was to evaluate the effect of the quadratus lumborum block (QLB) on intraoperative opioid consumption in patients undergoing percutaneous biliary drainage (PBD).

Materials and methods: A total of 57 patients who met the inclusion criteria were randomized into two groups: the sedation group (Group 1, n = 26) and the sedation with QLB group (Group 2, n = 26). The groups were compared in terms of intraoperative anesthetic consumption, vital parameters, postanesthesia care unit (PACU) recovery times, postoperative visual analog scale (VAS) scores, postoperative analgesic consumption, and clinician and patient satisfaction. The study was …

Comparing Age Of Obesity Onset, Concerns About Weight And Body Shape, And Quality Of Life In Individuals Seeking Bariatric Surgery ​, Vi̇ldan Çakir Kardeş, Özge Saraçli, Nuray Atasoy, Levent Ati̇k, Si̇bel Kahraman Gi̇rgeç

Turkish Journal of Medical Sciences

Background/aim: This study explored the relationship between concerns about weight and body shape and quality of life in bariatric surgery candidates with morbid obesity regarding the onset of the weight problem in childhood, adolescence, or adulthood.

Materials and methods: We included 453 patients who applied to our center for preoperative bariatric surgery assessment between January 2010 and April 2019. We reviewed the sociodemographic information, psychiatric diagnoses, and results of the Body Shape Questionnaire-34, Quality of Life Scale, Beck Depression Inventory, and Beck Anxiety Inventory of all patients.

Results: Concerns about weight and body shape were increased in women (p < 0.001), unemployed patients (p = 0.001), those with a comorbid psychiatric disorder (p < 0.001), and those with childhood- or adolescent-onset of weight problems (r = –0.170; p < 0.001). Concerns about weight and body shape had a relationship with the vitality (r = –0.434), mental health (r = –0.464), social functioning (r = –0.418), pain (r = –0.307), and general health (r = –0.352) subscale scores of quality of life. Physical functioning was higher (p = 0.037) in patients with childhood- or adolescent-onset obesity compared to patients with adult-onset obesity, while mental health was higher (p = 0.001) in the latter group. Individuals with childhood- or adolescent-onset obesity inquired about bariatric surgery options at an earlier age (p < 0.001).

Conclusion: …

Hounsfield Unit Thresholds In Differentiating Adenoma From Nonadenoma Adrenal Masses: A Retrospective Adrenalectomy-Based Study ​, Burak Özbaş, Muhammet Mustafa Etleç, Hüseyi̇n Dursun, Mustafa Gök, Abdullah Bahadir Öz, Alper Celal Akcan, Ayşa Hacioğlu, Ökkeş İbrahi̇m Karahan, Züleyha Ci̇han Özdamar Karaca, Kürşad Ünlühizarci

Turkish Journal of Medical Sciences

Background/aim: Adrenal incidentalomas are increasingly detected during imaging studies performed for various indications, and their frequency in endocrinology practice has risen in recent years. While adenomas account for approximately 80% of adrenal incidentalomas, the remaining 20% include lesions such as primary malignancies, metastases, and pheochromocytomas that should not be overlooked. Given the risks associated with adrenal biopsy, accurate diagnosis with noninvasive methods is essential. In this retrospective study, we evaluated the Hounsfield unit (HU) values and lesion sizes measured on noncontrast computed tomography (CT) to identify a more accurate threshold than the widely used 10 HU.

Materials and methods: In …

Association Between Mri-Based Tumor-To-Skin Distance And Axillary Lymph Node Metastasis In Breast Cancer ​, Hi̇kmet Pehlevan Özel, Seçi̇l Gündoğdu, Eda Şahi̇ngöz, Ebru Menekşe, Mesut Tez

Turkish Journal of Medical Sciences

Background/aim: Axillary lymph node (ALN) status is a major prognostic factor in breast cancer and influences both survival outcomes and treatment planning. Noninvasive predictors of nodal metastasis are of clinical interest. Tumor-to-skin distance (TSD) has been proposed as a potential anatomical marker; however, standardized data based on magnetic resonance imaging (MRI) remain limited.

Materials and methods: This retrospective study included 98 female patients with pathologically confirmed breast cancer who underwent preoperative breast MRI between 2020 and 2025. Patients with prior breast or axillary surgery, distant metastasis, multifocal or multicentric disease, or inadequate MRI quality were excluded. TSD was measured on …

Direct Pars Repair Techniques For Lumbar Spondylolysis: A Comparative Literature Review ​, Kami̇l C. Kose, Moustafa Ibrahim, Ni̇cholas D. Haden, John Afolayan, Hanny Anwar

Turkish Journal of Medical Sciences

Background/aim: Lumbar spondylolysis, a pars interarticularis defect affecting approximately 3%–6% of the general population, presents a significant clinical challenge in young adults and athletes. When conservative management fails, direct pars repair offers an attractive alternative to spinal fusion by preserving segmental motion and reducing the risk of adjacent segment degeneration. This narrative review compares seven principal direct pars repair techniques—Buck’s technique, Scott’s wiring method, Morscher’s hook-screw system, pedicle screw-based constructs, the V-rod/U-rod (Gillet technique), the smiley face rod construct, and the dualstability construct (DSC)—evaluating their biomechanical properties, clinical outcomes, fusion rates, and complication profiles.

Materials and methods: A narrative literature …

Comparative Analysis Of Corticosteroid Injection Alone Versus Combined Corticosteroid And Radiofrequency Ablation For Plantar Fasciitis: Short- And Medium-Term Outcomes ​, Nevci̇han Şahutoğlu Bal, Emel Başar, Hati̇ce Babaoğlan, Ülkü Sabuncu, Mustafa Cem Yilmaz, Şükri̇ye Dadali, Erkan Yavuz Akçaboy

Turkish Journal of Medical Sciences

Background/aim: Plantar fasciitis (PF) is a common cause of heel pain and may lead to functional impairment and reduced quality of life. This study aimed to compare the short- and medium-term clinical outcomes of corticosteroid injection (CSI) alone versus combined radiofrequency ablation (RFA) and CSI in patients with PF refractory to conservative treatment.

Materials and methods: This single-center retrospective comparative study included patients diagnosed with PF between 1 January 2021 and 1 January 2025. Patients who underwent either CSI alone or combined RFA and CSI after failure of conservative management were included. Pain intensity was assessed using a visual analog …

Changes In T-Cell Subsets And Their Relationships With Disease Severity In Patients With Alopecia Areata, Hati̇ce Türk Daği, Hülya Özdemi̇r, Mehmet Akyürek, Fatma Tunçez Akyürek, Hasi̇be Artaç

Turkish Journal of Medical Sciences

Background/aim: Alopecia areata (AA) is a nonscarring hair loss disorder characterized by genetic susceptibility, environmental triggers, and T-cell–mediated autoimmunity. While various CD4+ and CD8+ T-cell subsets are implicated in its pathogenesis, the roles of follicular T-cell (Tfh), peripheral T-cell (Tph), and regulatory T-cell (Treg) subsets– particularly within the CD8+ population–remain incompletely defined. This study aimed to characterize circulating CD4+ and CD8+ T-cell subsets in Turkish patients with AA and to evaluate their associations with disease activity, duration, and severity.

Materials and methods: A total of 40 patients with AA and 40 healthy controls were enrolled. CD4+ and CD8+ T-cell subsets—including …

Histomorphological Changes In Human Septal Cartilage Stored In Different Preservation Solutions ​, Özlem Akkoca, Aki̇f Si̇nan Bi̇lgen, Harun Çoban, Fatma Aslan Yay, Zeynep Kaptan, Funda Balkiliç Akkaya, Rahmi̇ Kiliç, Sami̇ Engi̇n Muz

Turkish Journal of Medical Sciences

Background/aim: To assess the histopathological effects of three preservation solutions (70% ethanol, povidone-iodine, and ciprofloxacin) on human septal cartilage obtained during septoplasty and to compare their preservation characteristics for potential future graft applications. Optimal preservation of septal cartilage is clinically important because well-preserved grafts may reduce the need for secondary cartilage harvesting and the associated donor-site morbidity while facilitating reconstructive procedures during revision surgery.

Materials and methods: A total of 90 septal cartilage specimens obtained from 30 patients undergoing septoplasty were allocated to three preservation groups and stored in the designated preservation solutions at +4 °C. Samples were examined histologically …

Rna Sequencing Detects More Challenging Cases Of Somatic Hypermutation In The Ighv Gene In Patients With Chronic Lymphocytic Leukemia ​, Fatma Arikan, Pinar Ata, Şenol Demi̇r, Meral Uluköylü Mengüç, Fahi̇r Özkalemkaş, Bedretti̇n Orhan, Özgür Mehtap, Arda Bayar, Asu Fergün Yilmaz, Ayşe Tüli̇n Tuğlular, Işik Atagündüz, Tayfur Toptaş

Turkish Journal of Medical Sciences

Background/aim: Chronic lymphocytic leukemia (CLL) exhibits marked biological heterogeneity, and immunoglobulin heavy chain variable (IgHV) mutation status remains a key prognostic biomarker. We evaluated patient IgHV status via RNA sequencing (RNA-seq) and examined real-life clinical outcomes in a retrospective CLL cohort.

Materials and methods: Eighty-seven adults diagnosed with CLL between 2020 and 2022 at three centers were included. Total RNA from peripheral blood underwent anchored multiplex polymerase chain reaction with the Immunoverse BCR-IGH IGKL v1.0 kit (Integrated DNA Technologies) and was sequenced on the MiSeq platform (Illumina). IgHV mutation status was determined according to ERIC 2022 guidelines, specifically regarding classification …