Digital Commons Network^™

The Place We Live Which Does Not Exist, Jonathon Parker Yontz

Electronic Theses and Dissertations 2020 - Present

The Place We Live Which Does Not Exist is a collection of stories examining the intersection of trauma and catharsis through a psychologically manufactured underground environment. By inhabiting the perspectives of multiple characters, each story builds toward a collective reality that exists parallel to our present. As the shortsightedness of hedonistic expression is shown, the viability of escapism as panacea is questioned, exploring the ends of a society increasingly dependent on reclusive tendencies.

Some Closure On The Relation Between Cfg And Peg, Nathan J. Bulloch

Theses and Dissertations

We work toward a practical, unified language formalism for structurally recursive languages by expressing the mathematical theories underlying context-free grammars with lookahead (CFGLA) in the Rocq theorem prover and leveraging CFGLA semantics to analyze interactions between between recursion and negative lookahead. We give examples of grammars with left-recursive negation cycles for the parsing expression grammar (PEG) variant due to Medeiros et al. and for the translation from PEG to CFGLA due to Miyazaki and Minamide. To study the interaction between left recursion and negative lookahead in a PEG context, we introduce FPEG, a left-recursive extension of PEG with Fitting semantics.

Changes In Internet Search Term Popularity In Elder Mistreatment (2018-2023): Infodemiology Study Of Google Trends Data, Nicholas Sevey, Melvin Livingston, Kristin Lees-Haggerty, Olanike Ojelabi, Randi Campetti, Jason Burnett, Carolyn E Z Pickering, David Hancock, Rachit Sabharwal, Michael Brad Cannell

Faculty, Staff and Student Publications

Background: Elder mistreatment (EM) is a significant public health problem that is frequently underdetected and underreported. Insufficient public recognition and engagement have been hypothesized as one contributor to this underreporting; however, few data sources exist to quantify public awareness or engagement with EM at the population level.

Objective: This study examined relative internet search interest in EM compared with other forms of abuse (child abuse and domestic violence) in the United States using Google Trends data.

Methods: We analyzed Google Trends data to compare the Relative Search Index (RSI) for the terms "elder abuse," "child abuse," and "domestic violence" in …

Illinois Valley Central High School Wind Ensemble And Symphonic Band: April 21, 2026, Matthew Chapman, Lauren N. Bobarsky, Colby Powers

School of Music Programs

This is the one hundred and eighty-fourth program of the 2025-2026 season.

Center for the Performing Arts

April 21, 2026

Tuesday Evening

8:00 p.m.

Board Sustainability Governance And Environmental Citizenship In Global Hospitality Firms: Associations With Environmental Performance And Firm Value, Leonard A. Jackson, Kendra F. Jackson, Randall Upchurch, Danqing Liu, Michail Toanoglou, Shelby Renee Meek

Plymouth Business School

Hospitality and tourism firms are central actors in sustainable tourism transitions because their operations are resource intensive and highly visible to consumers and local communities. This study examines whether board-level governance mechanisms—board independence, gender diversity, a sustainability committee, CEO duality, and board size—are associated with environmental performance, and whether environmental performance is related to firm value in global hospitality firms. Using a panel of 10 large publicly traded hospitality companies across North America, Europe, and Asia from 2013–2022 (100 firm-year observations) and fixed-effects estimation, we find positive associations between board independence, board gender diversity, and the presence of a sustainability …

Cover And Contents

Turkish Journal of Chemistry

No abstract provided.

Computational And Experimental Analysis Of Oleanolic Acid As An Allosteric Activator Of Sirt1, Emrah Sariyer, Eda Dokumacioğlu, Hati̇ce İskender

Turkish Journal of Chemistry

Sirtuin 1 (SIRT1) is a member of the sirtuin protein family and the biological effects of SIRT1 encompass various areas such as the ageing process, metabolism regulation, and cellular stress response. SIRT1 activators are compounds that enhance the activity of SIRT1 enzyme. These activators can be natural or synthetic compounds and have the potential to provide health benefits by augmenting the biological effects of SIRT1. Oleanolic acid (OA) is a triterpenoid compound that has antiinflammatory, antioxidant, and glucose and lipid metabolism-regulating properties naturally found in many plants. The aim of the present study was to investigate the effect of OA …

Investigation Of The Enzyme-Inhibitory, Antibacterial, And Anticancer Properties Of Metal Phthalocyanines, Meryem Topal, Fevzi̇ Topal, Firat Yilmaz, Esra Bulut Atalay, Ümi̇t Muhammet Koçyi̇ği̇t, Emre Güzel

Turkish Journal of Chemistry

In this study, the enzyme inhibitory, antibacterial, and anticancer activities of a phthalonitrile derivative and its metal phthalocyanines (Pcs; indium, zinc, copper, cobalt, and manganese; 1–6) were investigated. The cobalt and manganese Pcs were synthesized for the first time in this study. The inhibitory activities of the symmetric Pcs, which were expected to interact with acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) via their 4-(isopropylbenzyl)oxy substituents through host–guest interactions, were investigated. More selective inhibition of AChE than BChE was observed for these compounds. For AChE, CoPc was identified as the most potent inhibitor (IC50 = 14.81 nM), whereas for BChE, InPc was …

Nucleophilic Cyclization Of N-Propargylated Pyrazole-5-Carboxylates: Synthesis Of Pyrazolo[1,5-A]Pyrazin-4(5h)-One Derivatives, Meli̇k Firat Mengeş, Meltem Tan Uygun

Turkish Journal of Chemistry

Cyclization reactions are among the most important and widely used methods for synthesizing biologically active fused heterocyclic compounds. In the present study, pyrazolopyrazinone derivatives (10a–i) were synthesized from N-propargylated pyrazoles via nucleophilic cyclization with hydrazine hydrate under a nitrogen atmosphere. Nuclear magnetic resonance (NMR) spectral analyses confirmed that ring closure proceeded in a manner consistent with the formation of a six-membered pyrazinone moiety. In addition, the pyrazolotriazepinone derivative 15c was synthesized using an alternative approach. In this method, substitution with chloroacetone was first carried out, followed by ring closure with hydrazine hydrate, affording a seven-membered triazepinone system. Comparison of the …

Sensitive Stripping Voltammetric Determination Of An Anthracene–Isatin Schiff Base In Biological Samples, Gökçe Öztürk, İrem Gökgöz, Emi̇ne Arslan, Eli̇f Şi̇şman, İnci̇ Seli̇n Doğan, Di̇lek Kul, Fatma Ağin

Turkish Journal of Chemistry

​In the present study, the electrochemical behavior and quantitative determination of the Schiff base 3-[(anthracen-2-yl)imino]-5-nitro-1,3-dihydro-2H-indol-2-one (ANT) were investigated for the first time using voltammetric techniques at a glassy carbon electrode. The effects of solvent composition, pH, and scan rate on the redox behavior of ANT were systematically evaluated. The compound exhibited an irreversible oxidation process governed by adsorption-controlled kinetics. Quantitative analysis was conducted using differential pulse stripping voltammetry (AdS-DPV) and square-wave stripping voltammetry (AdS-SWV) under optimized conditions. Both methods exhibited linear responses over the concentration range of 0.2–8.0 μM, with limits of detection of 0.0366 μM and 0.0433 μM for …

Influence Of Precursor Synthesis Temperature And Hydrothermal Alkaline Medium On Agglomeration And Crystal Phase Of Barium Titanate Nanoparticles, Murat Özen

Turkish Journal of Chemistry

Phase‑pure, cube‑like barium titanate (BaTiO3) nanoparticles were synthesized via hydrothermal treatment of a single‑source barium-titanium peroxo-hydroxide precursor. Precursor solutions prepared at room temperature, 0 °C, and –10 °C with various storage times exhibited Ba/Ti molar ratios ranging from 0.66 to 1.05 as determined by X-ray fluorescence. Cooling the precursor solution to –10 °C effectively suppressed the polymerization of Ti-O-Ti, yielding near‑ideal stoichiometry and finer precursor particles. Subsequent hydrothermal processing in NH4OH, KOH, and NaOH (0.5–10 M, 100–200 °C) revealed the strong dependence of agglomeration and morphology on the alkaline medium. While NH4OH and KOH resulted in severe agglomeration and the …

Templating Effect Of Neutral Dithiane Ligands On The Self-Assembly Of Cationic Au(I)-Phosphine Units, Farzane Osanlou, Abdollah Neshat, Mohammad Reza Yousefshahi, Vaclav Eigner, Michal Dusek, Shahram Seidi

Turkish Journal of Chemistry

The interactions between the dinuclear Au(I) precursor (dppm)Au2Cl2 and neutral sulfur-containing dithianes (specifically 1,3- and 1,4-dithiane) were examined in the presence of NH₄PF₆. Although these sulfur-based ligands were initially employed to facilitate the formation of mixed phosphine/sulfur Au(I) complexes, single-crystal X-ray diffraction analysis revealed the unexpected generation of multinuclear Au(I) assemblies exclusively coordinated by phosphine ligands. When 1,3-dithiane was present, a trinuclear Au(I) complex was isolated, characterized by a central four-coordinate Au(I) center exhibiting a distorted see-saw geometry, flanked by two terminal Au(I) centers with T-shaped coordination environments. Importantly, the dithiane ligand did not coordinate directly to the gold centers …

Vanadium’S Journey From Essential Trace Metal To Emerging Environmental Pollutant In The Anthropocene Era, Tanu Arora, Nivedita Agnihotri, Mohammad Azam

Turkish Journal of Chemistry

Vanadium, previously considered a minor trace element, is gaining global environmental attention due to its expanding applications in metallurgy, energy storage, catalysis, and agriculture. With this rise in its industrial and technological uses, however, anthropogenic emissions of vanadium into the atmosphere, soils, and water bodies have also escalated. Unlike many heavy metals, vanadium exhibits complex redox behavior, primarily existing as V(IV) and V(V) species, which significantly impacts its environmental mobility, bioavailability, and toxicity. Vanadium exhibits complex behavior that necessitates an integrated research approach to better understand its various roles in scientific fields. This review summarizes the current knowledge on vanadium’s …

The Impact Paradox: Mixed-Methods Evaluation Of National Institute Of Health And Care Research Funding For Intellectual Disability Research In The Uk, Rohit Shankar, Richard Laugharne, Sarah Lennard, Samuel Tromans, Nathan Goddard, Madeline Dale, Ruth Bishop, Nathan Johnson, Maxine Hough

Peninsula Medical School

BackgroundPeople with intellectual disability experience substantialhealth inequities, including higher multimorbidity, increasedhealthcare utilisation and markedly reduced life expectancy.High-quality research is essential to address these disparities.The National Institute for Health and Care Research (NIHR)funded Research Delivery Network provides the infrastructure/expertise/support needed to deliver NIHR-funded studies, andsupports studies funded by a non-commercial/industry partner. However, the effectiveness of NIHR-funded studies versusthose supported in driving impactful intellectual disabilityresearch remains unclear.AimsTo evaluate and compare the outcomes of NIHR-funded andsupported intellectual disability research.MethodAll NIHR studies (funded/supported) relating to intellectualdisability (2010–2020) were identified through systematicregister searches. Primary outcomes included publicationrates and impact on local, national and international clinicalguidelines. …

Enhancing Lane Detection In Autonomous Vehicles Using Data Augmentation For Adverse Environmental Conditions, Rutvikkumar Dave

Electronic Theses and Dissertations 2020 - Present

To make sure that self-driving and connected automobile technologies are safe and work well, it’s really important that they can correctly identify lanes. But lane detection Algorithms typically have a hard time working well when the weather is bad, such when it rains, fogs, or goes too fast. The circumstances cause visual distortions that make existing computer vision systems less reliable, which makes it harder requires autonomous navigation systems to work well. This paper introduces a comprehensive lane detection system that integrates synthetic Weather-informed data augmentation combined with a Weather-aware Temporal Lane Detection Network (WTLDNet) to make it easier for …

The Hate Circuit: A Collection Of Essays On Hate And Healing, Vivian Murphy

Electronic Theses and Dissertations 2020 - Present

The Hate Circuit: A Collection of Essays on Hate and Healing is a lyric essay collection tracing the neuroscience, psychology, and lived experience of hatred and its slow unraveling. Rooted in the author’s relationship with her alcoholic mother, the essays move between memoir and scientific inquiry, examining how hatred forms in the brain, how it is inherited across generations, and what it costs to sustain it. Drawing on research in neuroscience, attachment theory, and the psychology of forgiveness, the collection argues that hatred is not simply an emotion but a circuit—one that can, with effort, be rewired. The essays ultimately …

The Scholastic Trombone Ensemble: Instructional Guide And Custom Arrangements For Secondary School Trombone Choir, Cassidy Clayton Shiflett-Cockrell

Electronic Theses and Dissertations Archive

ABSTRACT The trombone ensemble is an increasingly popular academic tool for young collegiate players to cultivate musicianship and ensemble skills. Because this setting exclusively uses trombones, it is excellent for training intonation, resonance, and overall blend. I was first introduced to performing in a trombone ensemble during my undergraduate studies at the University of North Alabama through a trombone quartet. The time I spent learning to listen and blend with my colleagues was foundational to developing the critical ensemble skills I still use frequently. Throughout my following studies at The University of Akron and the University of Memphis, respectively, the …

Intensive Versus Standard Blood Pressure Control After Endovascular Thrombectomy In Acute Ischemic Stroke: A Meta‐Analysis Of Randomized Controlled Trials, Tallal Mushtaq Hashmi, Mushood Ahmed, Hadiah Ashraf, Muhammad Shakir, Ibrahim Ahmad Bhatti, Faizan Ahmed, Ahmad Alareed, Ali Hasan, Gregg C. Fonarow, Ameer E. Hassan

School of Medicine Publications

Background: The optimal strategy for blood pressure management following mechanical thrombectomy for acute ischemic stroke due to large vessel occlusion remains a subject of ongoing research.

Methods: We comprehensively searched PubMed, Embase, and the Cochrane Library from inception to June 20, 2025. Randomized controlled trials comparing intensive versus standard blood pressure control following mechanical thrombectomy for acute ischemic stroke were included. The primary outcomes were excellent functional outcome (modified Rankin Scale score 0-1 at 90 days) and good functional outcome (modified Rankin Scale score 0-2 at 90 days). Pooled odds ratios (ORs) with 95% CIs were calculated using a random-effects …

The Diamond Minutes: Rethinking The Earliest Link Of The Trauma Chain Of Survival, Guglielmo Imbriaco, Sonia D’Arrigo, Francesco Limonti, Tim Nutbeam, Alberto Cucino

Peninsula Medical School

Matters arising.Trauma remains one of the leading causes of death worldwide, particularly among children, adolescents, and working-age adults. Despite major advances in trauma system and hospital-based care, a significant proportion of fatalities occur in the prehospital setting, often within minutes and before arrival of emergency medical services (EMS) [1]. Observational analyses consistently suggest that between one quarter and nearly half of trauma-related deaths occur before hospital arrival [2]. A recent prospective multicentre study involving over 1,000 patients with haemorrhagic shock demonstrated that, despite improvements in trauma centre capabilities, including whole blood resuscitation, hybrid operating rooms, and endovascular haemorrhage control, the …

Be Trauma Informed: A Pilot Evaluation Of A Podcast Intervention To Improve Trauma-Informed Care Knowledge, Attitudes, And Practices Among Pediatric Critical Care Nurses, Elizabeth Devries

Dissertations (1934 -)

Trauma-informed care (TIC) is integral to pediatric critical care (PCC) but accessible, scalable education is limited. This mixed-methods pilot study developed and evaluated Be Trauma Informed, a two-episode podcast educational series designed to increase PCC nurses’ TIC knowledge, attitudes, and trauma-informed practices while also assessing implementation outcomes of feasibility, necessity, acceptability, fidelity, safety, and beginning effectiveness. The curriculum for the podcast series was developed by the primary investigator with stakeholder input from PCC and TIC experts. The podcast was professionally produced, and episodes were distributed on major podcast platforms. Using purposive convenience sampling, PCC nurses within the United States completed …

Using Chatgpt Ai To Analyze Clinical Judgment In Immersive Virtual Reality Simulation From Prelicensure Nursing Students, Shari Ann Fehrenbach

Dissertations (1934 -)

This retrospective, descriptive study examined how clinical judgment was demonstrated across two immersive virtual reality simulation (IVRS) experiences in a cohort of Direct Entry Master of Science in Nursing (DEMSN) students using a generative large language model artificial intelligence, ChatGPT, for categorization of clinical judgment cognitive processes. The National Council of State Boards of Nursing (NCSBN) Clinical Judgment Measurement Model (CJMM) guided the study (NCSBN, 2022). Nurse expert-derived CJMM-aligned scoring rubrics were applied to deidentified, developer-generated IVRS performance reports, and ChatGPT categorized whether rubric actions were demonstrated and generated case-level, scored excel files. Total CJMM and cognitive component scores were …

Morphology Extraction And Analysis Of Primary Soot Particles And Aggregates And Aggregates In Soot Tem Images Using Machine Learning, Timothy Paul Day

Master's Theses (2009 -)

Accurate characterization of soot morphology is crucial for understanding soot’s physical and chemical properties and their effects on climate and public health. Morphological characterization requires manual identification and segmentation of individual primary particles and aggregates, a labor-intensive and time-consuming process limits scale and reproducibility and contributes to uncertainty in climate models. Therefore, automated approaches are needed to reliably quantify soot morphology. This work develops SAGE (Soot Aggregate Geometry Extraction), an instance segmentation model and framework to segment primary particles in TEM images and extract morphological properties. Four key questions are addressed: (1) can the model achieve segmentation quality comparable to …

Prodromal Lewy Body Disorder Features In Rem Sleep Behavior Disorder With Biomarker-Defined Synucleinopathy, Daniel Weintraub, Michele K York, Roseanne Dobkin, Anuprita R Nair, Ryan Kurth, David-Erick Lafontant, Chelsea Caspell-Garcia, Roy N Alcalay, Ethan G Brown, Lana M Chahine, Christopher Coffey, Tatiana Foroud, Douglas Galasko, Karl Kieburtz, Kenneth Marek, Kalpana Merchant, Brit Mollenhauer, Kathleen L Poston, Andrew Siderowf, Cristina Simonet, Tanya Simuni, Caroline M Tanner, Thomas F Tropea, Aleksandar Videnovic, Parkinson's Progression Markers Initiative

Faculty, Staff and Students Publications

Objective: Isolated rapid eye movement sleep behavior disorder (iRBD) is a prodromal state for Lewy body disorders and exhibits biological heterogeneity that may influence clinical expression and progression. We examined clinical features in individuals with iRBD and biomarker-defined synucleinopathy.

Methods: Parkinson's Progression Markers Initiative (PPMI) is a longitudinal, multi-center observational study. Participants included polysomnogram (PSG)-confirmed iRBD individuals who were cerebrospinal fluid (CSF) α-synuclein seed amplification assay positive with no clinical diagnosis of Parkinson's disease or dementia with Lewy bodies, along with robust healthy controls (HCs). Clinical and biological features of prodromal PD and DLB, including mild cognitive impairment (MCI), subthreshold …

Development Of Telenutrition Education And Counseling Protocol To Improve Diet Quality In Individuals With Spinal Cord Injury, Adrianne Widaman, Benjamin Dirlikov, Ramya Gopalan, Shelley Wood, Maria Sol Casas, Kazuko Shem

Faculty Research, Scholarly, and Creative Activity

Background: Cardiometabolic disease (CMD) is a leading cause of death for individuals living with chronic spinal cord injury (SCI), with a mortality rate due to CMD being higher than individuals without SCI. Telenutrition (TN) interventions tailored to health and accessibility needs of individuals with SCI may mitigate cardiometabolic risks. Objectives: To describe the development and validation of a telehealth-based medical nutrition therapy (MNT) within a clinical trial investigating the effectiveness of modifying diets to improve cardiometabolic health in persons with chronic SCI. Methods: The adaptive module-based MNT education content was developed in 3 phases by a multidisciplinary team. The first …

Comparing The Effects Of Suicide Stigma Reduction Interventions On Adult Help-Seeking Intentions: An Experimental Investigation, Max Stivers

LSU Master's Theses

With relatively few individuals who experience suicidal thoughts and/or behaviors (STB) engaging in formal treatment, one critical aspect of bridging the treatment gap is to reduce barriers to their seeking help (WHO, 2014). Stigma regarding suicide and mental health help-seeking has been identified as a barrier to care, inspiring development of interventions and public health campaigns targeting stigma reduction as a means of increasing help-seeking attitudes, intentions, and behavior (Corrigan et al., 2004). However, because efforts focusing specifically on reducing stigma may fail to address other logistical and psychosocial barriers to engaging with treatment, their broader impact in promoting help-seeking …

Holding The Line: The Existential Threat To Dei Language And Values, Terrance Fitzsimmons, Larry Martinez, Jennifer Bishop, Darryl Rice, Florence Villesèche

Psychology Faculty Publications and Presentations

Purpose – This article critically analyses and critiques the operationalisation of the Trump administration’s anti- DEI policies on Diversity, Equity and Inclusion (DEI) initiatives in the United States of America and beyond. It specifically examines the attempt to silence DEI scholarship and practice, assessing the broader implications for social justice and global progress towards equity. The article also positions these attacks as an existential threat to DEI scholarship, scholars and practitioners, with the potential to reverse progress made since the adoption of the Universal Declaration of Human Rights in 1948. The article serves as a call to action, urging the …

Designing Next-Generation Cardiometabolic Outcome Trials For Obesity Medicines, Elke Platz, Alexander Peikert, Biykem Bozkurt, Bart Van Der Schueren, Javed Butler, Ildiko Lingvay, Darren K Mcguire, Faiez Zannad, Naveed Sattar

Faculty, Staff and Students Publications

The prevalence of obesity has reached pandemic proportions and is having enormous public health effects. Obesity increases the risk of type 2 diabetes, hypertension, chronic kidney disease, and cardiovascular (CV) disease including coronary artery disease, heart failure, atrial fibrillation, and stroke. Although initial randomized clinical trials of weight-loss strategies through diet and exercise or early medical therapies did not lead to improved CV outcomes, more recent trials using glucagon-like peptide-1 (GLP-1) receptor agonists in individuals with obesity have demonstrated CV benefits. At present, there are multiple cardiovascular outcome trials (CVOTs) of novel GLP-1 receptor agonists and investigational products targeting multiple …

Rhesus Macaques With An Opa1 Mutation Demonstrate Features Of Autosomal Dominant Optic Atrophy, Tracy N Jaggers, Ana Ripolles-Garcia, Ala Moshiri, Brett D Story, Jun Wang, Rui Chen, Lucy G Moore, Leandro B C Teixeira, Jaeho Shim, Ana C Raposo, Maria Isabel Casanova, Sophie M Le, Sangwan Park, Laura J Young, Soohyun Kim, Karolina P Roszak, Vanessa Ureno, Paige M Karpinen, Nayeli Echeverria, Monica Ardon, Brian C Leonard, Marguerite Knipe, Eliza Bliss-Moreau, Brad Fortune, J Timothy Stout, Jeffrey Rogers, Nicholas Marsh-Armstrong, Sara M Thomasy

Faculty, Staff and Students Publications

Autosomal dominant optic atrophy (ADOA) is an inherited optic neuropathy primarily caused by mutations in OPA1. We identified and defined a spontaneous nonhuman primate (NHP) model of ADOA using rhesus macaques heterozygous for a missense mutation (OPA1A8S). With ocular examinations, ophthalmic imaging, electroretinography, histopathology, immunohistochemistry, and transmission electron microscopy (TEM), we documented retinal nerve fiber layer (RNFL) thinning, retinal ganglion cell (RGC) loss and dysfunction, OPA1 mislocalization, and reduced axonal mitochondrial density in affected macaques. Our investigation revealed substantial phenotypic variability among affected macaques, shedding light on the pathogenesis of ADOA. The retinas were evaluated using techniques …

Out-Of-Pocket Costs And Antihypertensive Medication Use Among Veterans: A Natural Experiment, Alexander R. Zheutlin, Alexander Chaitoff, Daniel Addo, Joshua A. Jacobs, Catherine G. Derington, Eric L. Stulberg, Peter A. Glynn, Bernardo Modenesi, Jordana B. Cohen, Adam P. Bress

Department of Neurology Faculty Papers

BACKGROUND: Blood pressure (BP) control remains suboptimal in the United States despite available low-cost antihypertensive medications. Even small out-of-pocket medication costs may contribute to adherence. We determined whether $0 versus >$0 out-of-pocket costs for antihypertensive medications is associated with differences in adherence, discontinuation, BP control, and cardiovascular outcomes.

METHODS: We included veterans with newly diagnosed hypertension who initiated antihypertensive medication in the Veterans Health Administration from 2004 to 2022. A fuzzy regression discontinuity design with 2-stage residual inclusion leveraged differences in medication copay policy at a 50% service-connected disability threshold (≥50% service-connected disability: $0 out-of-pocket cost versus < 50%: $8 monthly cost). Outcomes included 1-year medication nonadherence (proportion of days covered < 80%), 1-year medication discontinuation (no medication in the final 90 days of the study year), 1-year BP control (systolic BP/diastolic BP < 140/90 mm Hg and < 130/80 mm Hg) and cardiovascular disease events (myocardial infarction, coronary revascularization, peripheral artery disease, or stroke).

RESULTS: Among 417 …

Enhancing Inference Of Differential Gene Expression In Metatranscriptomes From Human Microbial Communities, Evan M Lee, Nathan P Mcnulty, Matthew C Hibberd, Jiye Cheng, Kazi Ahsan, Hao-Wei Chang, Barak A Cohen, Jeffrey I Gordon

2020-Current year OA Pubs

Metatranscriptomic (MTX) sequencing quantifies gene expression from the collective genomes of microbial communities (microbiomes), enabling assessment of functional activity rather than functional potential. While differential expression testing is essential for RNA-sequencing analysis, current metatranscriptomic approaches have only been benchmarked on simulated data, resulting in a lack of standard practices for analysis of real datasets. Here, we use mock communities (defined mixtures of microbial cells with known properties) to quantitatively assess robustness and susceptibility of current approaches to various confounders including organisms' low relative abundance, differential abundance, low prevalence, global transcriptional output changes, and compositional effects. We show that no current …