Digital Commons Network^™

High-Density Lipoprotein-Associated Cholesterol Abnormalities In A Clinical Outcomes Study Of Dysferlin-Deficient Limb-Girdle Muscular Dystrophy Type R2, Zoe White, Alan Pestronk, Et Al.

2020-Current year OA Pubs

BACKGROUND: Limb-girdle muscular dystrophy (MD) type R2 (LGMDR2, formerly LGMD2B) is an autosomal recessive form of MD caused by variants in the dysferlin gene, DYSF. It leads to slow proximal and distal muscle weakening that generally results in loss of ambulation around early adulthood but without the lethal cardiorespiratory dysfunction observed in the more severe Duchenne MD. How loss of dysferlin causes muscle fibre death is poorly understood, but recent evidence suggests a link between muscle wasting and loss of muscle cholesterol homeostasis with circulating lipoprotein abnormalities in many forms of MD.

METHODS: Cross-sectional circulating total cholesterol (CHOL), high-density lipoprotein-associated …

Characteristics Of Responders To Interventions For Parkinson Disease: A Scoping Systematic Review, Sidney T. Baudendistel, Gammon M Earhart

2020-Current year OA Pubs

AIMS: The purpose of this review is to identify the characteristics of responders in interventions targeting motor function for individuals with Parkinson disease.

MATERIALS & METHODS: The primary search included, 'Parkinson' + 'responder.' A second, broader, search further included 'response' + 'responsiveness' + 'responsive.' Records were sorted by intervention: neuromodulation, pharmaceutical, physical, and placebo.

RESULTS: Thirteen studies were identified in the primary and 19 studies in the secondary search, culminating in 120 characteristics. For neuromodulation interventions, responders were younger at onset, more responsive to levodopa, and had more difficulties with activities of daily living. Responders to pharmaceuticals were younger at …

The Evolution Of The High Redshift Quiescent Population In Cosmos-Web, Carter B. Flayhart

Theses

Understanding the quiescent galaxy population is incredibly important in the context of galaxy evolution. These galaxies are defined as having a low star formation rate, and their evolved stellar populations makes them identifiable by their redder colors in both the observed and rest-frame. Since their rest-frame optical emission lies in the infrared (IR) regime, observations of these galaxies at high redshifts were quite difficult prior to the launch of the James Webb Space Telescope (JWST). Recent observations of this high redshift population conflict with galaxy formation and evolution models that existed prior, with quiescent galaxies being detected more frequently than …

Autophagy Regulates Müller Glial Cell Inflammatory Activation, Teresa A Doggett, Zhenqing Zhou, Sohini Rebba, Jacqueline Unsinger, Philip A Ruzycki, Thomas A Ferguson

2020-Current year OA Pubs

PURPOSE: We tested whether Müller cells utilize autophagy to support immune privilege in the eye.

METHODS: The essential autophagy gene Atg5 was deleted in retinal Müller cells. Inflammation was induced by intravitreal injection of lipopolysaccharide (LPS) that was monitored by hematoxylin and eosin (H&E) staining, immunofluorescent confocal microscopy, and flow cytometry. Single-cell RNA sequencing was performed on retinal Müller cells isolated from control and Atg5-deficient mice. Markers of Müller cell gliosis were assessed, and cytokine production in the eye was measured. Small interfering RNA knockdown techniques were used to examine LPS-induced inflammatory pathways in culture.

RESULTS: We observed increased and …

Longer Scans Boost Prediction And Cut Costs In Brain-Wide Association Studies, Leon Qi Rong Ooi, Scott Marek, Nico U F Dosenbach, Timothy O Laumann, Evan M Gordon, Et Al.

2020-Current year OA Pubs

A pervasive dilemma in brain-wide association studies

Barriers And Facilitators To Accessing Healthcare For People With Parkinson's Disease In Latin America: A Qualitative Study, Christine Jeyachandran, Catherine Spooner, Ana Margarita Rodriguez Salgado, Matthew Prina, Joel Rhee, Jorge Jesus Llibre-Guerra, Dani Kim, Juan J Llibre-Rodriguez, Mark F Harris

2020-Current year OA Pubs

AIMS: The aim of the study was to identify the barriers and facilitators to healthcare access for people with Parkinson's disease (PWP) in Spanish-speaking Latin American countries (LAC) in Central and South America, to understand their drivers and consider the implications for health systems in LAC.

METHODS: Four online semi-structured focus groups were conducted with 25 PWP who provide education and/or support to PWP. The study was designed and implemented by a person with lived experience of PD. Data were mapped to an existing model of access to healthcare that incorporates provider and consumer abilities.

FINDINGS: There were multiple provider …

Formation Control Between Leader And Migratory Follower Tissues Allows Coordinated Growth, Toru Kawanishi, Takamichi Sushida, Tony Y-C Tsai, Hiroyuki Takeda, Sean G Megason

2020-Current year OA Pubs

Coordinated growth of multiple tissues is fundamental to shaping our body, but the underlying mechanisms remain underexplored. In zebrafish embryos, midline tissues composed of the notochord, floorplate, and hypochord elongate synchronously with their lengths aligned. We show that floorplate and hypochord cells collectively migrate posteriorly along the nascent notochord extracellular matrix as it extends posteriorly, maintaining the tripartite configuration. Fibroblast growth factor-mediated migration in a spatially graded manner causes cell stretching, which triggers Yap-dependent proliferation and controls floorplate and hypochord growth. Supported by mathematical modeling, we further suggest that their growth is fine-tuned by mechanical tethering to the notochord via …

Sex-Related Measurement Bias In Autism Spectrum Disorder Symptoms In The Baby Siblings Research Consortium, Catherine A Burrows, Natasha Marrus, Et Al.

2020-Current year OA Pubs

IMPORTANCE: Disparities exist in age of diagnosis and prevalence of autism spectrum disorder (ASD) for female compared with male children. Correcting for sources of bias is critical for improving equitable ASD identification.

OBJECTIVE: To determine whether sex differences exist in measurement of ASD symptoms using the Autism Diagnostic Observation Schedule (ADOS) among young children at high familial likelihood (HFL) and low familial likelihood (LFL) of ASD.

DESIGN, SETTING, AND PARTICIPANTS: This cohort study collected longitudinal, prospective data from the Baby Siblings Research Consortium between January 1, 2003, and December 31, 2021. Participants included 3106 children who had an older sibling …

Disruption Of The Cerebrospinal Fluid-Plasma Protein Balance In Cognitive Impairment And Aging, Amelia Farinas, Daniel Western, Jigyasha Timsina, Carlos Cruchaga, Et Al.

2020-Current year OA Pubs

The brain barrier system, including the choroid plexus, meninges and brain vasculature, regulates substrate transport and maintains differential protein concentrations between blood and cerebrospinal fluid (CSF). Aging and neurodegeneration disrupt brain barrier function, but proteomic studies of the effects on blood-CSF protein balance are limited. Here we used SomaScan proteomics to characterize paired CSF and plasma samples from 2,171 healthy or cognitively impaired older individuals from multiple cohorts, including the Global Neurodegeneration Proteomics Consortium. We identified proteins with correlated CSF and plasma levels that are produced primarily outside the brain and are enriched for structural domains that may enable their …

Dissemination And Implementation Of A Telehealth-Enabled Program For Providing Infectious Disease Expertise In Rural Settings, Robin L P Jump, Jason P Burnham, Et Al.

2020-Current year OA Pubs

BACKGROUND: Telehealth can facilitate improving antibiotic use and related antimicrobial stewardship activities in facilities underserved by infectious diseases (IDs) expertise. We describe implementation of the Department of Veterans Affairs (VA) videoconference antimicrobial stewardship team (VAST) to connect multidisciplinary teams from rural VA medical centers (VAMCs) with geographically distant ID experts.

METHODS: We implemented VASTs at 10 rural VAMCs from September 2021 to February 2024. VASTs consisted of regular meetings between rural VAMCs and an off-site ID expert who met by videoconference to discuss clinical cases, emphasizing recommendations for antibiotic prescribing. The VA Corporate Data Warehouse was used to collect information …

The Global Neurodegeneration Proteomics Consortium: Biomarker And Drug Target Discovery For Common Neurodegenerative Diseases And Aging, Farhad Imam, Muhammad Ali, Carlos Cruchaga, Chengran Yang, Et Al.

2020-Current year OA Pubs

More than 57 million people globally suffer from neurodegenerative diseases, a figure expected to double every 20 years. Despite this growing burden, there are currently no cures, and treatment options remain limited due to disease heterogeneity, prolonged preclinical and prodromal phases, poor understanding of disease mechanisms, and diagnostic challenges. Identifying novel biomarkers is crucial for improving early detection, prognosis, staging and subtyping of these conditions. High-dimensional molecular studies in biofluids ('omics') offer promise for scalable biomarker discovery, but challenges in assembling large, diverse datasets hinder progress. To address this, the Global Neurodegeneration Proteomics Consortium (GNPC)-a public-private partnership-established one of the …

The Microrna Mir-30a Blocks Adipose Tissue Fibrosis Accumulation In Obesity, Pradip K Saha, Robert Sharp, Aaron R. Cox, Rabie Habib, Michael J. Bolt, Jessica B. Felix, Claudia E. Ramirez Bustamante, Xin Li, Sung Yun Jung, Kang Ho Kim, Kai Sun, Huaizhu Wu, Samuel Klein, Sean M. Hartig

2020-Current year OA Pubs

White adipose tissue (WAT) fibrosis occurring in obesity contributes to the inflammatory and metabolic comorbidities of insulin resistance and type 2 diabetes, yet the mechanisms involved remain poorly understood. Here, we report a role for the broadly conserved miRNA miR-30a as a regulator of WAT fibrosis and systemic glucose metabolism. Mice modified to express miR-30a at elevated levels in adipose tissues maintain insulin sensitivity coupled with reduced fatty liver disease when fed a high-fat diet. These effects were attributable to cell-autonomous functions of miR-30a that potently increase expression of adipocyte-specific genes. Proteomic screening revealed miR-30a limits profibrotic programs in subcutaneous …

Multimodal Deep Learning For Enhanced Breast Cancer Diagnosis On Sonography, Ting-Ruen Wei, Aileen Chang, Young Kang, Mahesh Patel, Yi Fang, Yuling Yan

Bioengineering

This study introduces a novel multimodal deep learning model tailored for the differentiation of benign and malignant breast masses using dual-view breast ultrasound images (radial and anti-radial views) in conjunction with corresponding radiology reports. The proposed multimodal model architecture includes specialized image and text encoders for independent feature extraction, along with a transformation layer to align the multimodal features for the subsequent classification task. The model achieved an area of the curve of 85% and outperformed unimodal models with 6% and 8% in Youden index. Additionally, our multimodal model surpassed zero-shot predictions generated by prominent foundation models such …

An Examination Of Legal Status, Law Enforcement Perception, And Crime Victimization And Reporting Among Hispanic Immigrants, Guadalupe C. Cabrera

Electronic Theses and Dissertations

This study relied on in-depth interviews with naturalized citizens and lawful permanent resident Hispanic immigrants to explore their perceptions of law enforcement and experiences with crime victimization and reporting. Past literature has explained that the absence of legal status may deter crime reporting. We added to this body of work by investigating relationships among legal status, trust, and reporting behaviors. This study finds that direct experiences with law enforcement and acquired knowledge may be a better indicator of law enforcement trust and crime reporting compared to legal status. While most participants expressed trust in law enforcement, others felt neutral due …

Empathetic Differentiated Instruction In Deaf Education: Examining Influencing Factors And Experienced Educator Practices, Laura A. Blackburn

STEMPS Master's Level Projects and Papers

This mixed-methods study investigated how experienced K–12 deaf educators implement empathetic, differentiated instruction (EDI) to support the academic and linguistic needs of deaf students who use American Sign Language (ASL) as their language of learning. Guided by Tracey and Baaki’s instructional design empathy model and grounded in constructivism, the study explored how teachers navigated the tension between standardized, English (sound-based) curriculum demands and the need to make instructional decisions aligned with signed language modalities and responsive to students’ real-time learning needs.

A custom electronic survey was distributed to a national sample of experienced educators, with 19 respondents completing Likert-scale items, …

Coastal Transition Zone Development Effects On Salt Marsh Ecology, Amanda Mei Pysher

Electronic Theses and Dissertations

The coastal transition zone (CTZ) is a regional gradient linking terrestrial and marine systems, comprising of the terrestrial upland, the marsh-upland ecotone, and estuarine salt marshes. Along all US coastlines, including the South Carolina (SC) coast from Little River to Georgetown, increases in anthropogenic development of terrestrial uplands have altered the historically established vegetation, and various physical, chemical, biological, and geological gradients. Despite the CTZ's ecological importance, the trickle-down effects of terrestrial upland development on associated high marsh habitats remain understudied. Data were collected from developed (DEV) and undeveloped (UND) shorelines across three SC estuarine inlets. Multilevel modeling (MLM) was …

Migratory Patterns Of Juvenile Shortfin Makos (Isurus Oxyrinchus): Evidence Of A Nursery In The Western North Atlantic, Julia A. Crews

Electronic Theses and Dissertations

Shortfin Makos, Isurus oxyrinchus Rafinesque, 1810, are lamnids that have a global distribution in temperate and tropical oceans. Locations of critical early life history events including birthing and nursery areas are unknown or unconfirmed for most populations. The objective of this study was to identify if a nursery area for Shortfin Makos in the northern waters of the Mid-Atlantic Bight using the criteria articulated by Heupel et al. (2007). Satellite tags were used to track the movement of juveniles (<200 cm TL, n=7) up to 5 months. Tag detections (n=882) confirmed that young makos stay in continental shelf waters of the northern Mid-Atlantic Bight off Long Island through the meteorological summer (June–August) and early meteorological fall (September–October) when sea surface temperatures are 18–23°C. There was clear migratory movement from juveniles once sea surface temperatures fell below 15°C. During late fall, some young sharks moved south towards Cape Hatteras, North Carolina, while one shark moved east up to 1,800 km into the open north Atlantic Ocean into meteorological winter. These movement data of juvenile Shortfin Makos combined with multi-year catches and sightings (2018–2024) provide strong evidence for a nursery area in the northern Mid-Atlantic Bight. By identifying a nursery area for Shortfin Makos, knowledge gaps that currently exist for this vulnerable species and support effective management can be closed.

Comparison Of Nutrient Concentrations And Primary Production Indicators Across Plume Fronts, Taylor Dishon

Electronic Theses and Dissertations

Coastal buoyant plumes carry low-salinity waters containing particulate and dissolved terrestrial materials with an abundance of nutrients into the coastal ocean, including inorganic nitrogen and phosphorous. Plume fronts act as transport barriers created from the convergence of a fresher plume with an older, saltier plume or with ambient waters, leading to potential accumulation of phytoplankton and nutrients. This accumulation creates an optimal environment for primary production. The primary goals of this study were: to investigate the distribution of and potential relationships between nutrient concentrations and chlorophyll a across plume fronts of the Winyah Bay River Plume, and examine potential relationships …

Site Fidelity, Seasonality, Movement, And Fish Associates Of Sand Tigers Carcharias Taurus In North Carolina, Ara L. Mcclanahan

Electronic Theses and Dissertations

Sand tigers Carcharias taurus are mackerel sharks with a disjunct global distribution in tropical and temperate seas. In the northwest Atlantic, sand tigers migrate along the coast of the eastern United States from the Gulf of Maine to Florida and occupy structurally complex benthic habitats. The long-term, underwater camera installation, SharkCam, at Frying Pan Tower, off Cape Fear, North Carolina, USA, sits atop an expanse of hard bottom reef frequented by sand tigers. The citizen-science platform Spot A Shark USA (SAS USA) uses images of sand tigers to identify and track individuals. This study utilized image encounters from SAS USA …

Perceptions Of First-Year College Students On Respect In The Classroom During Their High School Career, Jane Adele Bailey

Electronic Theses and Dissertations

This study examines the influence of teacher respect on classroom climate, student- teacher relationships, and academic performance. Through interviews with college students reflecting on their high school experiences, this research identifies specific teacher actions and communication that foster respect in the classroom. By identifying what respectful communication and actions are through the interviews, teachers will be able to use these in their classrooms.

Six themes were identified by the participants lived experiences. They are: teacher demeanor, social interaction, attitude, emotional support, instructional management and facilitation, and listening. The results gave specific actions and communication, in the eyes of the participants, …

Adolescent E-Cigarette Expectancies: Measure Development And Preliminary Validity Of The Electronic Nicotine Vaping Outcomes Measure For Youth, Paul T. Harrell, Kelli J. England, Tracey E. Barnett, Vani N. Simmons, Richard W. Handel, Amy C. Paulson

Department of Pediatrics Faculty Publications

BACKGROUND: Electronic nicotine delivery systems (“e-cigarettes”) are the nicotine product most commonly used by adolescents. Research, treatment, and policy could benefit from measures of adolescent e-cigarette beliefs about outcomes of use (ie, expectancies). In the current study, we developed and tested an adolescent electronic nicotine vaping expectancy measure.

METHODS: A focus group with adolescents evaluated potential e-cigarette expectancy items. A panel of national experts assisted in revision of these items. Finally, items were administered to a sample of adolescents 14-17 years old (N = 267, Mean age 15.6, SD = 1.1, 50.9% Female, 50.2% Non-Hispanic White, 22.5% Non-Hispanic Black, 14.2% …

A Multi-Isotope Investigation Of Mammoth Diet, Habitat, And Movement Patterns In The Central Great Plains, U.S.A., Jacob Mccloskey

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

During the Last Glacial Maximum (LGM), the Great Plains in Nebraska consisted of wooded parklands and grasslands, whereas spruce (Picea) forests, parklands, and steppe grasslands dominated east of the Missouri River. I analyzed bulk carbon (δ¹³C), oxygen (δ¹⁸O), and strontium (⁸⁷Sr/⁸⁶Sr) isotopes from tooth enamel in isolated mammoth (Mammuthus) molars to compare diet and movement patterns in two late Pleistocene assemblages: one in Nebraska (n = 24), and one in western Iowa and Missouri (n = 17). Furthermore, I identified a morphometric subset of molars as …

Low-Level Polarimetric Signatures Of Supercells During Ef3+ Tornado Production, Anthony Noah Began

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

Most EF3+ tornadoes are produced by supercells. Understanding of microscale features surrounding intense tornado production remains limited. This research aims to determine commonalities in low-level microphysical processes during intense tornado production via polarimetric radar signature assessment with a Python-based algorithm. Polarimetric variables hypothesized to reflect low-level wind profiles such as the differential reflectivity (Z_DR) arc and the Z_DR–K_DP (specific differential phase) separation vector will be investigated. Supercells which produced EF3+ tornadoes will be examined since these tornadoes pose the highest threat. From 2010 to 2024, the United States recorded approximately 19,100 tornadoes which resulted in …

Adolescent Online Leisure, Media Literacy, Online Victimization, And Multifaceted Well-Being During Covid-19: The Role Of Parental Support, Yunqi Wang

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The rapid advancement of the internet and digital technology has provided opportunities for adolescents to conduct leisure activities online on a daily basis. However, research on adolescent technology use and mental health often highlights negative effects without carefully examining the specific mechanisms, which overlooks the complexity of effects of online behaviors on adolescent well-being and the importance of developing adolescents’ media literacy skills. Moreover, despite the crucial role of family dynamics in adolescent development, research on how parental support and involvement might affect adolescent technology use and well-being is still limited. The current study retrieved data from Programme for International …

Evaluation Of Performance Characteristics And Groundwater Contamination Risks Associated With On-Farm Swine Carcass Disposal Via Composting And Shallow Burial With Carbon, Gustavo Castro Garcia

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation evaluates the performance of three on-farm swine carcass disposal methods: whole carcass composting (WCC), ground carcass composting (GCC), and shallow burial with carbon (SBC), focusing on biosecurity factors and groundwater contamination risks. Foreign animal diseases (FADs), such as African swine fever and classical swine fever, pose severe economic and animal well-being risks if introduced to the United States. With confirmation of an FAD, swine movement will be halted, creating an urgent need for practical, biosecure, and environmentally responsible on-farm disposal strategies for swine carcasses. Nebraska, as a leading swine-producing state, exemplifies the vulnerability of high-density livestock regions to …

Online Prediction Of Streaming Data, Aleena Chanda

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

We present two new approaches for point prediction with streaming data based on a) the Count-Min sketch and b) Gaussian Process Priors with random bias. The methods are intended for the most general case where no true model can be usefully formulated for the data stream. In statistical contexts, this is often called the M open problem class. For the Count Min Sketch method we show that the predicted distribution function ^F converges to F under the assumption that the data consists of i.i.d samples from a fixed distribution function F. To implement the Gaussian Process Prior methods, we used …

Hormonal Modulation Of Sexual Approach And Avoidance Among Women With A History Of Sexual Trauma, Anneliis Susan Sartin-Tarm

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

A history of sexual trauma places women at a high risk for sexual dysfunction and other health sequelae, yet the influence of ovarian hormones in this population is poorly understood. This dissertation tested the theory that the effects of ovarian hormones are mediated by cognitive approach-avoidance systems to shape sexual well-being, and that this process is moderated by sexual trauma history. This was investigated across three studies examining the menstrual cycle, hormonal contraceptive use, and menopause. Across each chapter, I present complementary evidence demonstrating that hormones interact with the interpretation of ambiguous internal and external cues with respect to sexual …

Reductive And Nucleophilic Cleavage Of Peroxides And Synthesis Of Tocotrienols As Radioprotective Targets, Amber Marie Schuster

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

I. SET CLEAVAGE OF PEROXIDES: INFLUENCE OF REDUCTANT AND PEROXIDE ON THE RATE AND MODE OF ALKOXY RADICAL FORMATION

The reductive cleavage of organic peroxides to generate alkoxy radicals is frequently accomplished via single-electron transfer (SET) from a reductant. Previous reports employ either symmetrical peroxides or peroxides with predictable cleavage behavior, such as peresters. We were interested in how the structure of the peroxide and the nature of the reducing system combine to impact the rate of reaction and the selectivity for alkoxy radical formation during cleavage of unsymmetric peroxides. In this dissertation, one will find the reactions of a …

Chronic Pain After Traumatic Brain Injury: Treatment Experiences And Effects On Cognition And Functioning, Rachael L. Snyder

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Traumatic brain injury (TBI) can contribute to changes in cognitive and emotional functioning, quality of life, and psychosocial reintegration. Chronic pain is a common consequence of TBI which can uniquely impact such outcomes. Still, the exact nature of the contribution of pain on post-TBI outcomes remains unclear, and there are few existing studies exploring the treatment journey for chronic pain and injury rehabilitation through qualitative methods, specifically individual interviews with TBI survivors. This study aimed to document these narratives and to identify precisely how the experiences of TBI survivors seeking treatments, rather than the treatments themselves, impact outcomes. It also …

How Developers Use Type-System Related Programming Language Features, Samuel W. Flint

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Optional type annotations are a popular feature of programming languages that allow developers to omit explicit type information in code while, in some cases, retaining many of the benefits of static typing, such as in-code documentation, improved detection of type errors, or enforcement of code properties. However, how developers use and understand optional type annotations is not clear. The focus of this dissertation is to understand the use and comprehension of optional type annotations.

Optional type annotations are examined through four lenses: first, by examining the evolution of usage in a statically typed programming language (Kotlin, the default language for …