Digital Commons Network^™

Engineering Mutation Clones In Mammalian Cells With Crispr/Cas9, Zijun Huo, Jian Tu, Rachel Shoemaker, Dung-Fang Lee, Ruiying Zhao

Faculty, Staff and Student Publications

CRISPR, Clustered Regularly Interspaced Short Palindromic Repeat, as a powerful genome engineering system, has been widely accepted and employed in gene editing of a vast range of cell types. Compared to zinc finger nucleases (ZFNs) or transcription activator-like effector nucleases (TALENs), CRISPR shows a less complicated process and higher efficiency. With the development of different CRISPR systems, it can be used not only to knock out a gene but also to make precise modifications, activate or repress target genes with epigenetic modifications, and even for genome wide screening. Here we will describe the procedure of generating a stable cell line …

Inclusive Psychology Pedagogy Is Linked To Increased Belonging And Reduced Prejudice Toward Consensually Non-Monogamous Relationships, Rose Bern, Rachel Dinero, Kate Linenbach, Amy Moors

Psychology Faculty Articles and Research

Students enrolled in an elective psychology course entitled Relationship Science, which emphasized inclusive curricula focused on relationship diversity, completed pre- and postcourse assessments of attitudes toward consensually nonmonogamous (CNM) relationships, relationship skills (e.g., constructive communication), and classroom belonging. Given the established literature on lesbian, gay, bisexual, transgender, queer+-inclusive pedagogy, attitudes toward lesbian, gay, bisexual, transgender, queer+ individuals were also measured. Comparisons of pre- and postcourse assessments revealed significant reductions in stigma toward individuals engaged in CNM, alongside increased acceptance of CNM relationships more broadly. Students also reported increases in relationship skills and classroom belonging over the course of the …

A Quantum Walk Through Sustainability: Nonlinear Thinking For A Nonlinear World, Sanjay Wahal

Trade & Scholarly Monographs

We are living through a planetary inflection point. Conventional sustainability thinking-rooted in linear logic, siloed disciplines, and incremental fixes-no longer suffices. The climate crisis is nonlinear. Our response must be as well.

In this book, Dr. Sanjay Wahal, global systems strategist and founder of Decarbonization, LLC, delivers a visionary, paradigm-shifting framework that draws inspiration from quantum theory to reimagine how we think, act, and lead for climate action.

This is not a book about quick fixes or silver bullets. It is a meditation on coherence, emergence, and transformation. It is for those who believe sustainability is not just a policy …

Students With Vision And Hearing Loss: Successful Strategies For Teachers And Families, Karen A. Waldron, Michael Steer, Dolly Bhargava

Education Faculty Research

Students with Vision and Hearing Loss: Successful Strategies for Teachers and Families is an eBook containing extensive online Activities, Research, and Resources to support children and adolescents who are Vision Impaired or Blind, and those who experience a Hearing Loss or are Deaf.

This collaborative project was administered by Dr. Karen A. Waldron (Trinity University, San Antonio, Texas) and Dr. Michael Steer, with the support of graduate student Dolly Bhargava (Renwick College, New South Wales).

It includes the work of many experts at universities, schools, and regional service centers across the United States, Australia, and New Zealand, and provides specific …

Hydesville Goose Prairie: Early Pioneers And The Making Of A Town, Jack Mchenry, Paula Gundlach Mchenry, Janet Corbett Mattson

Trade & Scholarly Monographs

This book is laid out as a timeline beginning when Hydesville was first being settled. Included are not only happenings in the area, but also events that were occurring outside the area that would have affect-ed or been of interest to Humboldt County residents. Great care has been taken to preserve the history of the area, to learn who the families were, their reason for coming to the area, and from where they originated. This book was compiled through the effort and interviews of the entire community. The McHenrys began collecting Hydesville history some sixty years ago. Jack McHenry wrote …

Errors And Misconceptions In Mathematics Teacher Retraining: An Investigation Of Subject Knowledge Enhancement Course Results, Gabor Szalontai

School of Law, Humanities and Social Sciences Theses

This thesis investigates the subject knowledge capabilities and deficiencies of teachers who wished to enter or return to secondary mathematics teaching and took part in the Subject Knowledge Enhancement Plus programme. It focuses on the proportions, types and causes of their mistakes, as well as their potential for development and the possibility of increasing their awareness of errors.The study uses recorded responses of 1602 participants from the online assessment of the courses, which ran across England. The assessment, covering the entire secondary curriculum and consisting of 643 questions, provided an interactive, multiple-attempt design allowing the investigation of the participants’ results …

“We're Just Glorified Babysitters.” : Early Years Educators' Perspective On Learning And Expertism, Hayley Fisher

School of Law, Humanities and Social Sciences Theses

This qualitative study explores early years educators’ perceptions of learning and expertise, emphasising how they experience, develop, and articulate their professional knowledge in early childhood education. Based on ten in-depth semi-structured interviews, it critically examines the often complex and conflicting ways educators view their roles, identities, and professional development. The research is rooted in adult learning theories, including Knowles’ (1972) andragogy, Kolb’s (1984) experiential learning model, and Mezirow’s (1997) transformative learning theory, alongside Eraut’s (1994) work on professional knowledge and expertise, which highlights the relational, practice-based, and social dimensions of knowledge formation. Through thematic analysis, four key themes emerged: expertee-ism, …

Exploring And Increasing Women’S And Girls’ Recreational Ocean Access In A Coastal Community In The Tropical Majority, Florence Barraud

School of Law, Humanities and Social Sciences Theses

Vast gender inequalities restrict women’s and girls’ access to ocean spaces, particularly in the tropical majority. Globally, 68% of women cannot swim, compared to 43% of men. In many coastal communities, women are key stewards of highly biodiverse yet vulnerable marine environments that people rely upon for food security, livelihoods, culture, and coastal protection. Being able to swim is imperative for enabling access to the dominant environment and a core recreational, educational, and economic space. This study aimed to explore and increase women’s and girls’ recreational ocean access in the Maldives, a tropical majority island nation.Utilising a pragmatic feminist approach, …

Safe Family Therapy Activities And The Emotional Well-Being Of Parents Of Autistic Children, Gamze Camci

School of Law, Humanities and Social Sciences Theses

This thesis addresses a critical gap in the existing literature on the emotional well-being of parents of autistic children by applying SAFE family therapy activities grounded in the Narrative Attachment Therapy approach through a three-cycle action research design. Stage 1 involved 3 parents as primary participants, with their children present during selected sessions. Stage 2 included 2 families; the primary focus was on 3 parents across these families. Stage 3 engaged 5 therapists. Data were collected through interviews, diaries, questionnaires and therapy recordings. Data from interviews and journals were analysed thematically, whereas questionnaires were analysed idiographically. The first and second …

Exploring The Reading Motivation And Reading Experiences Of Secondary School Students With Dyslexia In England, Ahmet Kaan Alper

School of Law, Humanities and Social Sciences Theses

This research examines how a small group of secondary school students with dyslexia in England make sense of their reading experiences and motivation to read across school, home and everyday settings. It responds to concerns that literacy policy and practice foreground accuracy, speed and comparison, leaving limited space for reading for pleasure on students’ own terms. Drawing on Michel Foucault’s concepts, the study treats dyslexia and literacy as historically and institutionally produced categories and explores how discourses, norms and accountability regimes shape what counts as legitimate reading and a motivated reader. The research is grounded in a relativist ontology and …

Social And Emotional Experiences Of Higher Education Students With Dyslexia In Türki̇Ye And England: Exploring The Effect Of Context, Levent Yigit

School of Law, Humanities and Social Sciences Theses

This research study examined the social and emotional experiences of university students with dyslexia in Türkiye and England who had received an official diagnosis at some point in their lives. Findings were compared and the differing experiences in two different countries highlighted. Six students in Türkiye and five students in England participated in the study. To produce an in-depth subjective dataset capturing life-stories, a qualitative narrative approach was adopted. Three, and for some participants four, consecutive semi-structured interviews were conducted, with at least a one-week interval between each interview. The study adopted a longitudinal approach as each interview investigated students’ …

The Effects Of Altered Partial Pressures Of Gas On Cognitive And Neural Function, Daniel Graham

School of Psychology Theses

Oxygen is central to neural function, yet the precise mechanisms and effects by which varying oxygen levels, whether through Hypoxia, Hyperoxia, or Hyperbaric Oxygen Therapy (HBOT), shape cognition and brain activity remain incomplete. This thesis adopts a novel, multi-modal framework that integrates normobaric gas manipulations, cognitive testing, Transcranial Magnetic Stimulation (TMS), Electroencephalography (EEG), Functional Magnetic Resonance Imaging (fMRI), and HBOT to examine how gas and pressure variability influences cognitive, motor, and neural processes. Chapter 3 presents the findings of a preliminary experiment investigating normobaric oxygen manipulations on cognition. Standardised cognitive assessments revealed domain-general impairments (i.e. memory and executive function) under …

Understanding Processes Involved In Firefighter/Ems Resilience And Suicidality: A Mixed Methods Analysis, Benjamin H. Barnette

Clinical Psychology Dissertations

First responders in the fire service and emergency medical services (EMS) are the subject of research focused on career suicide risk, occupational hazards, and psychological resilience; however, the nature of the relationships between these three variables is not well understood. This study employed a mixed methods design to test the moderating role of resilience in the relationship between the severity of critical incident exposure and two distinct processes involved in suicidality: suicidogenic cognitions and the acquired capability for suicide. A total of 248 eligible participants with experience in firefighting and/or EMS responded to an online survey. Psychological resilience was negatively …

Community-Based Urban Forestry: Creating And Sustaining Community Partnerships Between Urban Forestry Non-Profit Organizations And Academic Institutions, Paloma Doyle

Botany Honors Papers

No abstract provided.

Oil Palm (Elaeis Guineensis) Leaf Extract-Mediated Green Synthesis Of Tio2 Nanoparticles And Their Optical Characteristics, Nofrijon Sofyan, Andriayani, Yetria Rilda, Fiona Angellinnov, Mouna M'Rad, Muhammad, Aga Ridhova, Akhmad Herman Yuwono, Donanta Dhaneswara

Baghdad Science Journal

This work aims to explore the characteristics of TiO2 NPs green-synthesized via an environmentally friendly method using oil palm (Elaeis guineensis) leaf extract as a green medium and capping agent by utilizing titanium tetra isopropoxide (TTIP) as precursor. The oil palm leaves were extracted with different solvent concentration variations. The natural extract was characterized using liquid chromatography-mass spectroscopy (LC-MS) and infrared spectroscopy for active chemical contents and functional groups. The obtained TiO2 NPs were also characterized using infrared spectroscopy (FTIR) for the functional groups, ultraviolet spectroscopy (UV-DRS) for the optical characteristics, and X-ray diffraction (XRD) for the phase …

Hematite/Nio/Graphene Composites: Enhancing Humidity Energy Harvesting Through Temperature Variations, Nurul Syafiqah Mohamed Mustakim, Dayana Kamaruzaman, Norfarariyanti Parimon, Mohd Khairul Ahmad, Suriani Abu Bakar, Mohamad Hafiz Mamat

Baghdad Science Journal

Harvesting energy from humidity is a new approach that has emerged as a potential renewable energy source. However, there are drawbacks to current humidity-based energy-harvesting technologies, such as the absence of a long-term conversion mechanism in hygroscopic materials. In this study, we developed hematite/nickel oxide/graphene (hematite/NiO/Gr) on a cellulose-based substrate as the hygroscopic material for humidity-to-energy applications. We successfully synthesized a novel hematite/NiO/Gr composite using a cost-effective sonicated solution immersion method at different synthesis temperatures. Results indicated that the prepared hygroscopic material is hydrophilic, with a water contact angle of 67.83°. The fabricated humidity-to-energy device utilizing these hygroscopic materials yielded …

Effect Of Ba2+ Substitution At A-Site On Structural, Electrical, And Magnetic Properties Of Sm0.5Ca0.5Mno3 Perovskite Manganite, Nuraina Hanum Abdulruhim, Rozilah Rajmi, Muhammad Zulharmi Taqzim Kamaruzzaman, Norazila Ibrahim

Baghdad Science Journal

Mixed-valence perovskite manganites have attracted considerable attention due to their intriguing properties, such as colossal magnetoresistance and charge ordering (CO). In this study, Sm_0.5Ca_0.5-xBa_xMnO₃ (x = 0, 0.10, 0.20, 0.30, and 0.40) manganites were synthesized via the conventional solid-state reaction method to investigate the effect of Ba²⁺ substitution on their structural, magnetic, and electrical properties. X-ray diffraction patterns confirmed that all samples were single-phase and crystallized in an orthorhombic structure with a Pnma space group. Rietveld refinement indicated an increase in unit cell volume with increasing Ba²⁺ concentration, suggesting the successful …

Mapping Robusta Coffee (Coffea Canephora) Cropping Systems In Uganda: A Two-Step Pixel And Sub-Pixel Based Approach With Sentinel-2 Data, Getachew Kebede, Bester Tawona Mudereri, Onisimo Mutanga, Tobias Landmann, John Odindi, Natacha Motisi, Fabrice Pinard, Henri E.Z. Tonnang, Elfatih M. Abdel-Rahman

All Peer-Reviewed Publications

Coffee is a highly valued commodity and a widely consumed beverage, playing an important role in global trade. However, coffee farming landscapes are increasing transitioning into smaller-scale agricultural setups. This transformation highlights the critical need for accurate classification and mapping of coffee cropping systems (CS), especially in countries like Uganda, where dense vegetation and complex terrain present substantial challenges to traditional land survey methods. Moreover, understanding the spatial distribution of Robusta coffee (Coffea canephora) CS is essential for developing site-specific management strategies, guiding extension services, and informing evidence-based policy decisions. To address this gap, the present study aimed to enhance …

Optimal Control Analysis Of Malaria Transmission In The Presence Of Insecticide Resistance And Climate Variability In Kenya, Lorna Chepkemoi, Titus Okello Orwa, Samuel Mwalili, Rachel Waema Mbogo, Steeven Belvinos Affognon, Daisy Salifu, Henri E.Z. Tonnang

All Peer-Reviewed Publications

Malaria remains a major public health concern in Kenya, where changing climatic conditions, insecticide resistance, and mosquito behavioral adaptations continue to challenge control efforts. This study develops and analyzes a climate-sensitive malaria transmission model that incorporates mosquito behavior, insecticide resistance, and vector–human ecological dynamics to identify optimal control strategies for the Kenyan context. The model’s well-posedness was established, the basic reproduction number (R0) computed using the next-generation matrix method, and the stability of equilibrium points assessed. Spatial analysis of R0 was performed using temperature and rainfall raster data to map transmission risk across Kenya under varying insecticide use scenarios. Results …

Life Cycle Assessment Of Marine Composite Recreational Craft Manufacturing And Operations, Badr Moutik

School of Engineering, Computing and Mathematics Theses

Marine composite recreational craft are growing in number. Sectoral roadmaps for sustainable development are increasing pressure to demonstrate life cycle environmental performance. Life cycle assessment (LCA) has been identified as a key strategic decision support tool. However, current practice is undermined by sparse, non-representative data and methodological inconsistencies. These weaknesses increase the risk of biased or misleading interpretation, constrain wider uptake of LCA, and limit the sector’s ability to respond credibly to tightening environmental sustainability expectations. This thesis assesses the cradle-to-grave environmental impacts of a market-representative glass-fibre reinforced plastic (GRP) motor yacht across 16 impact categories. The research builds on …

Mapping The Uk Commercial Fishing Network: A Knowledge-Based Framework For Sustainable Technology Adoption And Environmental Decision-Making, Matthew Prowse

School of Engineering, Computing and Mathematics Theses

The UK commercial fishing industry operates within a complex web of stakeholders, technologies, and environmental pressures that challenge traditional approaches to sustainability and resource management. This research develops a comprehensive framework for understanding how technological advancement in UK fisheries creates unintended consequences that threaten long-term sustainability. Through a mixed-methods approach combining PESTLE analysis, grounded theory methodology, stakeholder mapping, case study investigation, this study introduces the "By-Product and Excess" theoretical framework to explain how fishing technologies generate two critical outcomes: by-products (undesirable environmental impacts such as CO₂ emissions, microplastics, and ecosystem damage) and excess (enhanced capacity to harvest beyond sustainable limits).The …

Modelling And Optimisation Of Dynamic Power Cable Configurations For Floating Offshore Renewable Energy, Anna Holcombe

School of Engineering, Computing and Mathematics Theses

Floating offshore renewable energy requires dynamic power cables to transmit generated electricity from the floating device to the seabed, or to an adjacent device, so that it can be exported to shore. Dynamic power cables are connected at the top end to a floating device, and traverse the water column, subjected to loading at the hang-off, and direct hydrodynamic loading from waves and currents. Their reliability has been a concern within industry and research, particularly due to historically high failure rates of static subsea cables. It is essential that design and analysis of dynamic power cables is accurate, to mitigate …

Oscillating Water Column Geometric Hybridisation And The Air Compressibility Effect, Gunay Gazaloglu

School of Engineering, Computing and Mathematics Theses

Oscillating Water Column (OWC) devices represent one of the most operationally robust wave energy conversion technologies, yet their commercial deployment remains hindered by narrow resonant bandwidth, geometry-dependent efficiency, and uncertainties in scaling thermodynamic effects between model and prototype. This thesis addresses these limitations through a coordinated investigation combining physical experimentation, empirical and machine-learning prediction, and high-fidelity computational fluid dynamics.A systematic experimental campaign was conducted on five single- and dual-chamber OWC geometries (Conventional, U-OWC, L-OWC, DCOWC-1, and DCOWC-2) under regular waves, with controlled compressible and incompressible chamber configurations. The thermodynamic scaling discrepancy is quantified for the first time across hybrid geometries, …

Degrees Of Separation: Grandchildren's Experiences Of Grandparental Brain Tumor Or Stroke, Emily Sapsford, Alyson Norman, Sue Jackson, Josh Deacon, Emily Ravenhill, Pete Keohane

School of Psychology

The impact of acquired brain injury on family members is far reaching. Research has identified experiences of trauma, ambiguous loss and burden of care. Limited research has addressed the ripple effect of brain injury across the wider family. The study aimed to gain a detailed insight into the lived experiences of grandchildren of those who have experienced stroke or brain tumor. Ten participants (eight female and two males aged between 18 and 29) took part in semi-structured interviews about their experiences of grandparental stroke or brain tumor. Data were analyzed using interpretive phenomenological analysis (IPA). Four themes were identified. The …

Lower Suicidal Ideation Linked To Easier Access To Urban Green Spaces During The Covid-19 Pandemic: A Nationwide Study In Japan, Zheng Xian, Tomoki Nakaya, Clive E. Sabel, Ryo Okubo, Kun Liu, Jinguang Zhang, Takahiro Tabuchi

School of Geography, Earth and Environmental Sciences

Urban green spaces (UGSs) have been linked to improved mental health and lower suicide rates, yet their protective role during large-scale crises remains underexplored. We examined associations of UGS accessibility with long-term and pandemic induced new-onset suicidal ideation during the early phase of the COVID-19 pandemic in urban Japan, using data from a nationwide health survey of 17,376 participants (August 2020). Modified Poisson regression models estimated prevalence ratios (PRs) for suicidal ideation by UGS accessibility (area, number, and type) within 5, 10, and 15-minute walking thresholds from home, controlling for individual and area-level confounders. UGS accessibility exhibited dose-response relationships with …

Direct And Indirect Metal Contamination Of Estuarine Sediments By Boat Paint Particles, Andrew Turner, Ho Kwan Cheung

School of Geography, Earth and Environmental Sciences

Surface and subsurface estuarine sediments close to a cluster of abandoned boats have been fractionated (between <250 and 5000 μm) and analysed microscopically for paint particles. Paints were encountered in all samples, with concentrations calculated for whole sediment samples up to 1450 per kg (dry weight). X-ray fluorescence (XRF) analysis of paint particles retained by the coarsest (>5000 μm) size fraction revealed heterogeneous concentrations of Ba, Cu, Pb, Sn and Zn that reflect pigments and antifoulants used in various recent and historical antifouling and above waterline boat paints. Analysis of sediments by XRF revelated heterogeneous distributions of these metals between locations, size fractions and depths, and among replicate measurements at different positions of the same sample. Consequently, no relationship with grain size (evaluated using Rb as a proxy) was evident and concentrations often greatly exceeded predicted effect levels for sediments. Re-analysis …

Feasibility Study On The Development Of Indiana’S Connected And Automated Vehicle Roadmap And Strategic Plan, Can Cui, Juanwu Lu, Yupeng Zhou, Abin Mathew, Jue Zhou, Ziran Wang, Yaobin Chen

JTRP Technical Reports

This study provides INDOT with critical insights into major OEMs’ connected and autonomous vehicle (CAV) development plans and related research and demonstration activities in several surrounding states, guiding infrastructure investment and policy decisions. The report delivers three outputs that guide the CAV technologies implementation in Indiana. First, it synthesizes the best regional practices and industry direction to distinguish what is feasible now from what requires further development. Second, it establishes a technical baseline that links priority applications to sensing and communications architectures with explicit attention to interoperability, security, and maintainability. Third, it defines an implementation framework that connects capital and …

Assessment Of Temperature Correction Factors For Falling Weight Deflectometer Deflections, Pablo Orosa Iglesias, Jin Li, Bongsuk Park, Seonghwan Cho, John E. Haddock

JTRP Technical Reports

This study presents the development of an improved temperature correction methodology for falling weight deflectometer (FWD) deflections in full-depth asphalt pavements. The objective was to address the limitations of existing correction methods by integrating viscoelastic pavement behavior, structural properties, and realistic thermal gradients. Field temperature data from instrumented sites in Indiana were used to train a machine learning model capable of predicting pavement temperature profiles in flexible pavements. These predictions informed a series of finite element simulations that captured pavement responses to FWD loading under a range of thermal and structural conditions. Temperature correction factors were then derived and modeled …

Research Support To Indot On Systemwide Asset Condition Assessment Using Connected Vehicle Data, Andrew Thompson, Jairaj Desai, Rahul Suryakant Sakhare, Darcy M. Bullock

JTRP Technical Reports

Crowdsourced pavement roughness data using connected vehicles now provides cost-effective, high-resolution condition monitoring in near real-time. This study evaluates how connected vehicle data can support effective investment prioritization across Indiana’s road network and improve upon traditional data sources using surveys that are often done annually or biennially. A key challenge is handling terabyte-scale geospatial roughness datasets and integrating them meaningfully into asset management workflows. A scalable data processing methodology is outlined to address this issue and is used for the subsequent analysis.

This report’s analysis uses more than 3 billion daily CV-estimated roughness measurements (IRICVe) from 2022–2025, in addition to …

Physics-Based Modelling Of Concrete Degradation Phenomena: Impact Of Construction Defects On Long Term Cost And Material Properties, Manuel Salmeron, Oscar D. Forero, Talha Faiz Mohammed, Kabir Shrivastava, Shirley J. Dyke, Julio A. Ramirez

JTRP Technical Reports

The quality of workmanship and adherence to specifications during the bridge construction phase of concrete decks, slabs, and continuous T-beams, are key factors on which future structural performance and durability hinge. If construction practices do not meet the required standards of quality, they may trigger early onset of deterioration processes that shorten the service life leading to unplanned interventions or unscheduled repairs, thus intensifying the need for maintenance actions and increasing costs during the service life of the bridge. Despite the consensus view that substandard construction practices reduce the performance of concrete structures, quantifying the impact is challenging. This difficulty …