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Dna-Fish Metaphase Spreads To Distinguish Extrachromosomal Dna From Homogeneously Staining Regions In Human Cancer Cell Lines, Lauren Marie Masters, Katelin Maria Hagstrom, Graham Scott Erwin Jul 2026

Dna-Fish Metaphase Spreads To Distinguish Extrachromosomal Dna From Homogeneously Staining Regions In Human Cancer Cell Lines, Lauren Marie Masters, Katelin Maria Hagstrom, Graham Scott Erwin

Faculty, Staff and Students Publications

Whole-genome sequencing identifies focal DNA amplifications with base-pair resolution but cannot determine whether amplified sequences reside on extrachromosomal DNA (ecDNA, also known as double minutes) or within chromosomally integrated homogeneously staining regions (HSRs). DNA fluorescence in situ hybridization (DNA-FISH) metaphase spreads remain the gold standard for distinguishing these amplification states at single-cell resolution. Here, we present a detailed protocol for DNA-FISH metaphase spreads using human cancer cell lines, encompassing cell culture, metaphase arrest, hypotonic treatment, fixation, chromosome spreading, fluorescent probe hybridization, and fluorescence imaging. The protocol incorporates intermediate quality-control steps to verify successful chromosome dispersion and optimize metaphase spread quality, …

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Penguasaan Tanpa Dasar Hak Atas Bangunan Milik Orang Lain Sebagai Bentuk Perbuatan Melawan Hukum (Studi Putusan Mahkamah Agung Nomor 651 K/Pdt/2020), Helena Ginting, Abdul Salam Jul 2026

Penguasaan Tanpa Dasar Hak Atas Bangunan Milik Orang Lain Sebagai Bentuk Perbuatan Melawan Hukum (Studi Putusan Mahkamah Agung Nomor 651 K/Pdt/2020), Helena Ginting, Abdul Salam

Lex Patrimonium

Disputes over the possession of buildings often originate from preliminary sale and purchase agreements that fail to result in a lawful transfer of rights. Legal issues arise when a down payment sale agreement is declared null and void due to the buyer’s failure to fulfill payment obligations, while physical possession of the seller’s property is continuously maintained without a valid legal basis. This article aims to analyze the legal standing of down payment sale agreements in relation to the transfer of rights over land and buildings, the status of possession following the nullification of the agreement, and the legal qualification …

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Advances In Gene Editing Strategies For Viral Disease Resistance In Pigs: Knock-Out, Knock-In, And Overexpression Approaches, Zhao Namula, Takeshige Otoi, Aya Nakai, Megumi Nagahara, Maki Hirata, Fuminori Tanihara Jul 2026

Advances In Gene Editing Strategies For Viral Disease Resistance In Pigs: Knock-Out, Knock-In, And Overexpression Approaches, Zhao Namula, Takeshige Otoi, Aya Nakai, Megumi Nagahara, Maki Hirata, Fuminori Tanihara

The Thai Journal of Veterinary Medicine

Highly contagious viral pathogens—such as porcine reproductive and respiratory syndrome, classical swine fever, and African swine fever—continue to pose serious threats to the swine industry and cause major global economic losses. Conventional disease control approaches, such as vaccination and biosecurity, are limited by viral heterogeneity and immune escape mechanisms. Gene editing technologies, most notably the CRISPR/Cas9 system, offer an innovative means of introducing innate viral disease resistance or resilience in pigs. The continuous improvement of these tools can provide a sustainable strategy to enhance animal welfare and global food security and reduce reliance on traditional control measures. This review provides …

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Melding Existing Models Of Government-Assisted Insurance To Create A Comprehensive Federal Cyber Insurance Program, Patrick Miskovsky Jul 2026

Melding Existing Models Of Government-Assisted Insurance To Create A Comprehensive Federal Cyber Insurance Program, Patrick Miskovsky

Student Journal of Information Privacy Law

This article discusses the need for federal policy in the cybersecurity insurance market, and it considers whether existing federal insurance programs could serve as models for a national cyber insurance regime.  After framing the issue through a discussion of current cyber risks, the article examines the structure and mechanics of three existing federal insurance programs: the Terrorism Risk Insurance Program, the National Flood Insurance Program, and the Federal Deposit Insurance Corporation.  The article concludes by noting that elements of each would be essential in a federal cyber insurance program, and that policymakers should consider the extent to which these models …

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The Final Payroll: Human Resources, Meet Machine Resources, Sarah Yee Jul 2026

The Final Payroll: Human Resources, Meet Machine Resources, Sarah Yee

Student Journal of Information Privacy Law

As artificial intelligence (AI) becomes increasingly embedded in employment practices, particularly in hiring and screening, the need for robust regulatory frameworks is urgent. This Article focuses on the growing use of AI in workplace decision-making, highlighting the risks of algorithmic bias and discrimination. It argues that while the United States lacks a unified legal approach to governing AI in employment, the European Union (EU) has taken a more proactive and comprehensive stance. Through instruments like the proposed EU AI Act and existing data protection laws such as the General Data Protection Regulation (GDPR), the EU imposes meaningful obligations on employers …

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The Structural Problem With Real-Time Crime Centers, Holly Merclich Jul 2026

The Structural Problem With Real-Time Crime Centers, Holly Merclich

Student Journal of Information Privacy Law

Since the terror attacks of September 11, 2001, the government has become fixated with public safety, oftentimes at the expense of individual privacy. The surveillance state expanded after this in the early 2000s, and the country’s first Real Time Crime Centers were born. Real Time Crime Centers are hubs where different technologies and information are fused and aggregated into one easy to use space. Data outputs from automated license plate readers, closed circuit television, and various law enforcement databases are aggregated into one single pane of glass view to achieve higher rates of case closures and increase the efficiency of …

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Blurred Lines: How Mandatory Arbitration Clauses In Termsof Service Agreements Undermine Privacy Law Frameworks, Owen Curnin Jul 2026

Blurred Lines: How Mandatory Arbitration Clauses In Termsof Service Agreements Undermine Privacy Law Frameworks, Owen Curnin

Student Journal of Information Privacy Law

Following the birth of the Federal Arbitration Act–which made arbitration agreements valid, enforceable, and irrevocable in courts of law–mandatory arbitration clause use in commercial, consumer, and employment contracts exploded. These types of clauses require parties to submit all disputes arising out of or related to a contract to an impartial third party. This third party, known as the arbitrator, reviews the facts, listens to the parties’ arguments, and makes a final, binding decision resolving the dispute. Corporations have long relied on mandatory arbitration clauses to resolve disputes privately and confidentially, often hoping to avoid the public scrutiny and accountability associated …

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A Framework For The Use Of Facial Recognition Technology In Policing: Is Your Face Equivalent To Your Ip Address?, Amirah Farooq Jul 2026

A Framework For The Use Of Facial Recognition Technology In Policing: Is Your Face Equivalent To Your Ip Address?, Amirah Farooq

Student Journal of Information Privacy Law

This paper argues that police use of facial recognition technology constitutes a “search” within the meaning of section 8 of the Canadian Charter of Rights and Freedoms and therefore requires prior judicial authorization. Part A provides the background for this argument and begins with a case study of Clearview AI to establish the regulatory gap in Canadian AI legislation. Part B develops this argument by relying on the rules of evidence, the Charter, and analogies drawn from current case law. Part C addresses counterarguments. Part D concludes with a summary of the proposed framework and emphasizes the importance of a …

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Like, Share, & Comply: Recommendation Algorithms & Ftc Enforcement Authority, Alexandra Logan Jul 2026

Like, Share, & Comply: Recommendation Algorithms & Ftc Enforcement Authority, Alexandra Logan

Student Journal of Information Privacy Law

As recommendation algorithms and generative artificial intelligence become central to digital platforms, the Federal Trade Commission has, in the past, increasingly signaled its intent to regulate emerging technologies under Section 5 authority to prevent unfair or deceptive acts or practices. This paper examines how companies may face Federal Trade Commission scrutiny when algorithmic design, personalization systems, or public representations result in consumer harm. Drawing on case studies involving TikTok, Facebook, Snapchat, and others, the paper explores the limitations of plaintiff litigation under Section 230 of the Communications Decency Act and examines this potential avenue of expanding federal administrative enforcement. It …

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The Bent Clinician: How Dental Work Is Reshaping Your Spine - And What To Do About It, Kelly Davis Pt, Dpt, Faaompt, Mt Jul 2026

The Bent Clinician: How Dental Work Is Reshaping Your Spine - And What To Do About It, Kelly Davis Pt, Dpt, Faaompt, Mt

New Orleans Dental Association News

Musculoskeletal disorders affect somewhere between 64 and 93 percent of dental professionals over the course of their careers. Neck pain, shoulder aches, low back fatigue — these aren’t just the cost of doing good work. They are predictable patterns that, with the right understanding, you can absolutely change.

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Noda News Summer 2026 Jul 2026

Noda News Summer 2026

New Orleans Dental Association News

New Orleans Dental Association (NODA) News includes regular columns with regional news impacting the New Orleans membership including: columns from the President and Executive Director, feature articles relating to the field of dentistry, association events and upcoming activities, component news, continuing education opportunities, and classifieds.

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One Year's Seeding: Looking Back On The Last Seven Years Of Maine's Progress Toward Rural Justice, Christopher Northrop, Seth Main Jul 2026

One Year's Seeding: Looking Back On The Last Seven Years Of Maine's Progress Toward Rural Justice, Christopher Northrop, Seth Main

Maine Law Review

Nationally, the crisis in access to legal services in rural areas continues to grow despite significant attention and efforts to reverse this troubling trend. This Article explores Maine’s efforts to address our legal deserts. The Article’s starting point is a retrospective look at our 2019 national symposium on rural justice and accompanying Maine Law Review issue. We will try to measure successes and failures, while also addressing the significant impact caused by the intervening pandemic. The Article will then shift to an analysis of the current landscape, focusing on the role of the University of Maine School of Law. We …

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The Other Right To Counsel: The Civil Guarantee In State Constitutions, Dmitry Bam Jul 2026

The Other Right To Counsel: The Civil Guarantee In State Constitutions, Dmitry Bam

Maine Law Review

When most people speak of the right to counsel, they speak in the singular. After the Supreme Court’s seminal decision in Gideon v. Wainwright, the right to counsel in criminal prosecutions has become one of our most cherished fundamental rights. It is a right ingrained in our culture. Even children can recite the mantra that the accused have the right to a lawyer, and if they can’t afford a lawyer, one will be provided to them free of charge. It is no surprise, then, that our constitutional focus is trained on the criminal defendant’s right to counsel. After all, there …

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Legal Deserts Over Time, Cory L. Dodds Jul 2026

Legal Deserts Over Time, Cory L. Dodds

Maine Law Review

Legal deserts—counties with exceptionally low attorney availability—shape housing stability, personal safety, health outcomes, and economic security in rural communities. Yet, national scholarship has not yet determined whether attorney scarcity is transient or durable and relies largely on cross-sectional snapshots and population-based headcounts benchmarked to the American Bar Association’s (ABA) threshold of one attorney per 1,000 residents. These measures can obscure temporal dynamics, overstate functional supply, and embed contested normative assumptions about adequacy. This Article offers a national, longitudinal, and definition-sensitive analysis of legal deserts using county-level data from the forty-eight contiguous states at three observation points. Attorney supply is measured …

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Optimized Wishlist-Based Gift Purchase Flow For Digital Storefronts, Caitlyn Milne Jul 2026

Optimized Wishlist-Based Gift Purchase Flow For Digital Storefronts, Caitlyn Milne

Defensive Publications Series

Gifting via wishlists is a multi-step process that requires manual data entry for item selection and adding to cart, recipient information, gift message, payment information, etc. This disclosure describes techniques that eliminate unnecessary intermediary steps in the purchase flow of digital goods to streamline and speed up gifting. The techniques allow users to view the wishlist of a target gift recipient and select a gift option to reach a gifting purchase flow. In the purchase flow, recipient-related information is automatically inferred from the source. By retrieving recipient information automatically, intermediary steps for manual data entry of recipient information and/or steps …

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Bootstrap Connectivity For Mobile Devices Without Sim Cards, Aguibou Barry, Tim Auer, Wendy Wang, Pankaj Kumar Jul 2026

Bootstrap Connectivity For Mobile Devices Without Sim Cards, Aguibou Barry, Tim Auer, Wendy Wang, Pankaj Kumar

Defensive Publications Series

In some geographies, the lack of ubiquitous WiFi can complicate electronic subscriber identity module (eSIM) adoption. In some geographies, carriers can mandate device original equipment manufacturers (OEMs) to enable bootstrap connectivity, but eSIM-based bootstrap connectivity can be expensive for OEMs. This disclosure describes techniques that leverage extensible authentication protocol/ transport layer security (EAP-TLS) to enable secure connectivity of a mobile device to a cellular or satellite 5G network without an eSIM or physical SIM (pSIM). Following successful EAP-TLS authentication, the carrier enables traffic between the EAP-TLS-authenticated user equipment (UE) and the relevant service (based upon carrier-defined specific network policy). In …

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The Vanishing Customer: A Framework For Analyzing Benefit–Burden Decoupling In Attention-Economy And Ai-Companion Transactions, David Lee Wise, Avan Lee Wise Jul 2026

The Vanishing Customer: A Framework For Analyzing Benefit–Burden Decoupling In Attention-Economy And Ai-Companion Transactions, David Lee Wise, Avan Lee Wise

Defensive Publications Series

This publication describes an analytical framework for identifying and evaluating benefit–burden decoupling in digital-service transactions, with particular application to attention-economy platforms and conversational AI-companion systems. The framework treats two observed phenomena — the relocation of the paying party from customer to product (wherein a user's attention, data, or disclosure is monetized while the user is denied the status of a served customer), and the relocation of responsibility from maker to user (wherein a provider retains the financial benefit of a design or deployment decision while attempting, via terms-of-service disclaimers and "sole-risk" framing, to shed the decision's downstream burdens) — as …

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Managing A Plurality Of Parallel Batches, Saeed Alrahma, Michael Butler, Matthew Markwell Jul 2026

Managing A Plurality Of Parallel Batches, Saeed Alrahma, Michael Butler, Matthew Markwell

Defensive Publications Series

Limited accelerator memory on mobile devices may restrict the concurrent execution of parallel large language model workloads. The capacity of the key‑value cache may often constrain the number and length of these tasks. A workload scheduling method is disclosed to manage these memory constraints. When the cache capacity is reached, the state of a specific workload is serialized and moved to an alternative memory location. This process frees cache slots, which allows other parallel tasks to proceed. Once memory is vacated by completed tasks, the serialized state is restored to an accelerator cache for continued processing. This approach enables the …

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Proactive Make-Before-Break Traffic Steering In Evpn Multi-Homing Using A Bgp Extended Community For Pre-Failure Link Degradation Signaling, Anonymous Jul 2026

Proactive Make-Before-Break Traffic Steering In Evpn Multi-Homing Using A Bgp Extended Community For Pre-Failure Link Degradation Signaling, Anonymous

Defensive Publications Series

A method and system for proactive Make-Before-Break (MBB) traffic protection in an Ethernet Virtual Private Network (EVPN) is disclosed. A first Provider Edge (PE) router monitors a physical-layer Bit Error Rate (BER) on a link to a multi-homed Customer Edge (CE) device. When the measured BER crosses a configurable threshold while the link remains operationally up, the first PE router originates a Border Gateway Protocol (BGP) UPDATE carrying a new transitive Extended Community that signals link degradation, the BGP UPDATE being attached to an EVPN Type 1 Ethernet Auto-Discovery per-Ethernet Segment route. Remote PE routers receiving the Extended Community migrate …

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System For Identifying Geospatial Features Using Language Model-Guided Segmentation, Mira Leung Jul 2026

System For Identifying Geospatial Features Using Language Model-Guided Segmentation, Mira Leung

Defensive Publications Series

Geospatial information systems can face challenges from incomplete data, such as missing locations for building entrances, and difficulties processing complex natural language queries. A system can dynamically identify geospatial features using a multi-stage process. For example, a large language model can interpret a user's natural language query and an associated image to identify a relevant point of interest. Subsequently, a specialized machine learning segmentation model can analyze a localized region of the image to delineate a specific feature, such as an entrance. This approach can facilitate the on-demand generation of geospatial feature coordinates in response to semantic requests, which can …

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United States V. Alfonso: The Eleventh Circuit's Choice To Follow The Tide Of Other Circuits In Deciding What Constitutes The High Seas, Jaidin Winsor Jul 2026

United States V. Alfonso: The Eleventh Circuit's Choice To Follow The Tide Of Other Circuits In Deciding What Constitutes The High Seas, Jaidin Winsor

Ocean and Coastal Law Journal

In United States v. Alfonso, three appellants challenged the constitutionality of the assertion of subject matter jurisdiction by the United States over a vessel involved in illicit drug smuggling activity within the Exclusive Economic Zone (EEZ) of the Dominican Republic. On appeal the United States Court of Appeals for the Eleventh Circuit rejected this challenge. The decision made by the Eleventh Circuit Court of Appeals followed the approach taken explicitly by the Fourth Circuit and indirectly by the First and Second Circuits. This decision affirmed the expansive reach of United States territorial jurisdiction and is likely to encourage other cCircuits …

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"Tac-Ing" On Coherence: Achieving Sustainable Baltic Sea Fisheries Management By Hooking Precautionary Science And Greater Transparency In Eu Total Catch Negotiations, Patrick Seroogy Jul 2026

"Tac-Ing" On Coherence: Achieving Sustainable Baltic Sea Fisheries Management By Hooking Precautionary Science And Greater Transparency In Eu Total Catch Negotiations, Patrick Seroogy

Ocean and Coastal Law Journal

This article argues that what makes the difference in success between these two regimes is how strictly the scientific advice underlying catch limits the advice is adhered to, and the influences allowed unto decisionmakers in the catch limit negotiation process. Whereas the United States uses the scientific advice about the MSY as a strict upper limit in determining annual catch limits, the EU does not. This allows stakeholders in the EU’s CFP governance, in particular fishing industry actors, to influence regulators to raise TACs above adequately precautionary levels in the name of “balancing” sustainable fishing with short-term economic interests. The …

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Alaska's Hidden Gems: Advocating For The Protection Of Deep-Sea Corals, Both In The Courtroom And Beyond, Lauren Beizer Jul 2026

Alaska's Hidden Gems: Advocating For The Protection Of Deep-Sea Corals, Both In The Courtroom And Beyond, Lauren Beizer

Ocean and Coastal Law Journal

Coral reefs are ecologically, financially, and aesthetically one of the world’s greatest resources. Off the coast of Alaska, many unique species of deep-sea cold-water corals exist and provide various public health benefits, contribute vital ecosystem functions to their surrounding environment, and serve as economic tourist attractions. Alaska’s corals reefs are exceedingly valuable to the earth due to their overall ecological value. However, these unique coral species significantly lack state and federal protections–which have recently been under consideration in several lawsuits at the federal level. American Oceans Campaign v. Daley and Oceana, Inc. v. National Marine Fisheries Service et al., are …

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Rape And Sexual Assault On The High Seas: A Case Of Murky Jurisdiction, Linny Kit Tong Ng Jul 2026

Rape And Sexual Assault On The High Seas: A Case Of Murky Jurisdiction, Linny Kit Tong Ng

Ocean and Coastal Law Journal

Rape and sexual assault on the high seas present a complex, dual-faceted challenge encompassing both legal and socio-cultural dimensions. The high seas are marked by jurisdictional ambiguity. International law assigns jurisdiction to the flag State of the vessel. However, many vessels are registered to States lacking adequate legal frameworks or the capacity to address such crimes effectively. While international law does allow for intervention by other States under certain principles of jurisdiction, the lack of legal clarity and political will often results in hesitation from non-flag States, except for the United States. This hesitance leaves victims without recourse. This Article …

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Alternative Marine Fuels And Mrv Liability Under Unclos Part Xii, Andrey Chernov Jul 2026

Alternative Marine Fuels And Mrv Liability Under Unclos Part Xii, Andrey Chernov

Ocean and Coastal Law Journal

Shipping’s decarbonization depends not only on adopting alternative fuels but also on the reliability of the monitoring, reporting, and verification (MRV) systems that underpin compliance. Ammonia, hydrogen, methanol, and liquefied natural gas (LNG) each introduce distinctive risks of error at the stages of certification, custody transfer, onboard measurement, and verification. At the same time, maritime law is increasingly data-driven. The International Maritime Organization’s (IMO) Energy Efficiency Existing Ship Index (EEXI) and Carbon Intensity Indicator (CII) depend on reported fuel-use data, while the European Union’s Emissions Trading System (ETS) and FuelEU Maritime Regulation impose direct financial penalties for inaccurate reporting. This …

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Editor's Page, Art Mcphee Jul 2026

Editor's Page, Art Mcphee

Witness

No abstract provided.

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Mental Health And Psychosocial Well-Being Of Domestic Workers In Dar Es Salaam, Tanzania: Why It Matters And Why We Should Make It A Priority, Magolanga Shagembe Jul 2026

Mental Health And Psychosocial Well-Being Of Domestic Workers In Dar Es Salaam, Tanzania: Why It Matters And Why We Should Make It A Priority, Magolanga Shagembe

Tanzania Journal of Sociology

This phenomenological study explores the mental health and psychosocial well-being of domestic workers aged 15–18 years in Dar es Salaam, Tanzania. Drawing on Bronfenbrenner’s ecological systems theory and Erikson’s psychosocial development theory, the study examines how domestic workers negotiate self-acceptance, personal growth, purpose in life, quality of relationships, environmental control, autonomy and resilience within unequal labor and care arrangements. Data were generated through in-depth interviews with 29 domestic workers and analyzed thematically. The findings reveal that mental health and psychosocial well-being among domestic workers are shaped through complex interactions between structural vulnerability, interpersonal relationships, migration experiences, economic necessity, and future …

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Rebuilding Tourism Resilience In The Post-Pandemic Era: Hotel Success Narratives In Tanzania, Godwin Lema, Judith Mshana Jul 2026

Rebuilding Tourism Resilience In The Post-Pandemic Era: Hotel Success Narratives In Tanzania, Godwin Lema, Judith Mshana

Tanzania Journal of Sociology

COVID-19 struck tourism more deeply than ever before and it is still less clear how tourist hotels in the remote setting of the global south navigated the crisis. Limited explored empirically and what remain less known is how the standard operating procedures were designed, perceived and effectively applied to inform resilience in specific tourist hotel setting. In this article we explore the role of Standard Operating Procedures (SOP) played in rebuilding tourist hotels resilience. Karatu district Tanzania touristic space situates study context. Methodological approach adopted is qualitative research design with integration of interview, observation and pertinent hotel statistics in comparing …

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Medicalisation And The Practice Of Labour Augmentation In Mwanza, Tanzania, Latifa Abdulla Mohamed, Richard Sambaiga, Karen Marie Moland, Siri Lange Jul 2026

Medicalisation And The Practice Of Labour Augmentation In Mwanza, Tanzania, Latifa Abdulla Mohamed, Richard Sambaiga, Karen Marie Moland, Siri Lange

Tanzania Journal of Sociology

Since the late 1900s, maternal health has increasingly been medicalised as a result of growing global concerns about poor maternal outcomes. An example of this medicalisation process is the use of oxytocin for labour augmentation. This practice of administering oxytocin to shorten the duration of labour is increasingly questioned. Feminist scholars have often questioned the routine use of oxytocin, depicting it as unnecessary and may constitute inappropriate use of the drug that undermines women’s autonomy and contributes to negative birth experiences. However, these feminist arguments overlook individual agency and the complex ways in which actors encounter and interact with oxytocin …

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Barriers To Effective Provision Of Guidance And Counselling Services To Students With Visual Impairment: Experience From Government Secondary Schools In Tanzania, Silas Njenje, Bernadetha Gabriel Rushahu, Lwimiko Salum Sanga Jul 2026

Barriers To Effective Provision Of Guidance And Counselling Services To Students With Visual Impairment: Experience From Government Secondary Schools In Tanzania, Silas Njenje, Bernadetha Gabriel Rushahu, Lwimiko Salum Sanga

Tanzania Journal of Sociology

No abstract provided.

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