Digital Commons Network^™

Production Of Calcium Reduced Micellar Casein Concentrate Powders And Their Use In Process Cheese Products, Anil Kommineni

Electronic Theses and Dissertations

Protein is an essential dietary component, and sufficient intake is vital in a healthy and balanced diet. Consumers are becoming increasingly aware of and knowledgeable about the role of protein in the diet. Two of the next-generation dairy protein ingredients isolated from milk are micellar casein (MCC) and milk-derived whey protein, isolated from skim milk using microfiltration (MF). Membrane filtration has been used extensively by the dairy industry to produce a variety of dairy ingredients from milk. MCC manufactured from freshly pasteurized milk can be directly consumed or as a supplement to fortify and enhance nutritional qualities in processed food …

Crispr/Cas9 Whole-Genome Screens Identify Genes Critical For Neutral Lipid Metabolism In Macrophages, Rifat Sultana

Electronic Theses and Dissertations

Lipid-laden macrophages contribute to atherosclerotic plaque formation in atherosclerosisrelated heart diseases. Thus, understanding formation and degradation of lipid would help identify therapeutic targets for the treatment of atherosclerosis. In the first chapter of this dissertation, I have explained the current understanding of cholesterol metabolism in macrophages and how mTOR could play a potential role in this process. Additionally, I have described how CRISPR/Cas9 whole-genome screens present an effective tool to identify genes regulating cholesterol metabolism including positive and negative regulators of lipid droplet formation and lipid droplet degradation. In Chapter 2, I have described the results of the CRISPR/Cas9 whole-genome …

Efficacy Of Nanomaterials And Biorationals On The Bacterial Leaf Streak Management In Wheat, Abraham Hangamaisho

Electronic Theses and Dissertations

Wheat (Triticum aestivum L.) is one of the most important cereal crops in the United States. Most wheat varieties are susceptible to bacterial leaf streak (BLS), a major disease caused by Xanthomonas translucens pv. undulosa (Xtpvu). BLS is challenging to manage since common chemicals do not provide adequate control. Nanomaterials and plant extracts have shown potential to provide a sustainable environmentally friendly control of animal and plant diseases. However, limited data are available on the efficacy of plant extracts and nanomaterials controlling BLS in wheat. The objective of this study was to evaluate the antibacterial activity of plant extracts and …

Genome-Wide Association Mapping And Genomic Prediction For Enhancing Fhb Resistance In Hard Winter Wheat, Jinfeng Zhang

Electronic Theses and Dissertations

Wheat is one of the most important staple crops providing 20% of energy for 35% of the world population. Fusarium head blight (FHB), primarily caused by the fugal Fusarium graminearum Schwabe, is a damaging disease in wheat that affects global wheat production every year and causes food safety issues. The disease not only reduces the grain yield and quality but also produces mycotoxin in the diseased kernels making them unsuitable for human consumption or as livestock feeds. Breeding FHB resistant cultivar is the most effective and economical approach to managing the disease. This study combines genome-wide association study (GWAS) and …

Genistein-Enriched Pig Gut Microbiota Library As A Potential Probiotic Consortium, Theresah Amponsah

Electronic Theses and Dissertations

Plant-based diets contain numerous flavonoid compounds that produce antibacterial effects and gut health improvement. Genistein is among the most abundant isoflavones present in a plant-based diet and can be found in high amounts in soy products (up to 1g Kg-1). We describe here a robust method to identify genistein tolerant and metabolizing bacteria in swine gut microbiota and to screen the strains that have antibacterial and immunestimulatory properties. Such strains could be developed as non-antibiotic alternatives to prevent enteric infections in pigs and improve gut immunity. To this end, a mini bioreactorbased system was used to enrich genistein metabolizing bacteria …

Disentangling The Complex Evolutionary History Of Invasive Russian Thistle: Phylogenetic Relationships, The Role Of Hybridization, And Public Engagement, Deva Raj Khanal

Electronic Theses and Dissertations

Invasive species are forces of environmental, economic, and social change with detrimental impacts on ecosystems, commerce, agriculture, and human health. However, we still have a poor understanding of the processes that could be driving invasive potential. Hybridization may contribute to the establishment and spread of invasive lineages, as it may enhance genetic variation and facilitate rapid adaptation. Focusing on Russian thistle (Salsola tragus s.l., family Amaranthaceae) and closely related species, my thesis aims to clarify phylogenetic relationships and investigate how hybridization has shaped the invasion dynamics of these lineages. Known as one of the fastest plant invasions in the United …

A Post-Pneumonia Epizootic Evaluation Of The Rapid City, South Dakota Bighorn Sheep Herd, Amanda N. Ensrud

Electronic Theses and Dissertations

Pneumonia is a major factor affecting populations of free-ranging bighorn sheep (Ovis canadensis) across western North America. Pneumonia can occur in large-scale epizootics, during which greater than half of the population typically dies. After these epizootics, surviving ewes continue to conceive and bear lambs. However, lamb recruitment may remain low due to periodic or annual pneumonia outbreaks causing high lamb mortality rates, sometimes greater than 90%. Our study focused on the Rapid City, South Dakota bighorn sheep (BHS) herd that has recorded pneumonia-induced population decline since 2009. The first objective was to improve lamb health and survival by identifying and …

Post-Fledging Habitat Selection And Movements Of Juvenile Mallards In The Prairie Pothole Region, Cynthia E. Anchor

Electronic Theses and Dissertations

The Prairie Pothole Region (PPR) of North America provides critical habitat for waterfowl across life stages, but anthropogenic changes to the landscape have negatively impacted habitat quality for waterfowl. The mallard (Anas platyrhynchos) is abundant and socioeconomically valuable in the PPR, as it is a preferred target species of waterfowl hunters. Extensive research has evaluated breeding ecology of mallards in the PPR, but the period between juvenile mallards fledging and migrating has rarely been studied. The post-fledging ecology of juvenile mallards is a vital consideration for comprehensive waterfowl management. Further, juvenile mallards are a significant portion of hunter harvest at …

Studying Vulnerable Populations Through An Epigenetics Lens: Proceed With Caution, Katie Saulnier, Alison Berner, Stamatina Liosi, Brian Earp, Courtney D. Berrios, Stephanie O.M. Dyke, Charles Dupras, Yann Joly

Manuscripts, Articles, Book Chapters and Other Papers

Epigenetics – the study of mechanisms that influence and modify gene expression – is providing unique insights into how an individual’s social and physical environment impact the body at a molecular level, particularly in populations that experience stigmatization and trauma. Researchers are employing epigenetic studies to illuminate how epigenetic modifications lead to imbalances in health outcomes for vulnerable populations. However, the investigation of factors that render a population epigenetically vulnerable present particular ethical and methodological challenges. Here we are concerned with demonstrating how, in targeting certain populations for epigenetic research, this research may be pathologizing socio-cultural and medical practices in …

Ecological Restoration Of Lead/Zinc/Copper Mine Tailings: Phytomanagement And Amendment Strategies To Enhance Substrate Functionality And Biomass Production, Mariam K. Al-Lami

Doctoral Dissertations

“The extreme physiochemical characteristics of mine tailings inhibit microbial processes and natural plant growth. Consequently, vast and numerous tailings sites remain barren for decades and highly susceptible to wind and water erosion. Phytostabilization is a cost-effective and ecologically productive remediation approach; however, tailings revegetation is generally challenging and must often be assisted with appropriate soil amendments. Amendments applied individually in greenhouse studies discussed herein revealed notable improvement in bioenergy crops growth only with biosolids treatments. Recalcitrant carbon amendments (biochar and humus) showed notable impact only on tailings physichochemical and hydraulic properties. Nevertheless, biosolids may not support sustained vegetation due to …

The Autophagic Protein P62 Is A Target Of Reactive Aldehydes In Human And Murine Cholestatic Liver Disease, Colin T Shearn, Aimee L Anderson, Michael W Devereux, David J Orlicky, Cole Michel, Dennis R Petersen, Colin G Miller, Sanjiv Harpavat, Edward E Schmidt, Ronald J Sokol

Faculty, Staff and Students Publications

Inflammatory cholestatic liver diseases, including Primary Sclerosing Cholangitis (PSC), are characterized by periportal inflammation with progression to cirrhosis. The objective of this study was to examine interactions between oxidative stress and autophagy in cholestasis. Using hepatic tissue from male acute cholestatic (bile duct ligated) as well as chronic cholestatic (Mdr2KO) mice, localization of oxidative stress, the antioxidant response and induction of autophagy were analyzed and compared to human PSC liver. Concurrently, the ability of reactive aldehydes to post-translationally modify the autophagosome marker p62 was assessed in PSC liver tissue and in cell culture. Expression of autophagy markers was upregulated in …

Determination Of Cotinine Concentration In Urine By Liquid Chromatography Tandem Mass Spectrometry, Allison Beckett

Master of Science in Forensic Science Directed Research Projects

Tobacco use can cause lasting health conditions which can lead to death. Tobacco exposure comes from obvious sources like smoking or chewing tobacco, however these are not the only administration methods. Secondhand smoke exposure can affect even those who choose not to expose themselves to tobacco, which also opens them to the health issues that come along with tobacco use. Tobacco contains many different compounds, one of which is nicotine (Kataoka, 2009). Although nicotine is the most abundant compound in tobacco, it does not stay in the body for long periods of time. Nicotine metabolizes into cotinine which is more …

What Works To Improve Outcomes For Kenya’S Adolescent Girls?, Population Council

Adolescents and Young People

The Adolescent Girls Initiative-Kenya is a study that evaluates the long-term impact of a multi-sectoral intervention targeted at adolescent girls aged 11 to 15 years from Kibera and rural Wajir. The intervention consisted of four different components: a community-based violence prevention program, an education conditional cash transfer (CCT), health-focused girls' empowerment clubs, and wealth creation for girls via financial education and savings activities. This brief looks at the impact of the two-year program in Wajir County four years after it ended.

Inpatient Coding System And Opportunities For Documentation Optimization: An Interactive Session For Internal Medicine Residents, Lindsey Jordan Gay, Doris Lin, Ziba Colah, Glynda Raynaldo

Faculty, Staff and Students Publications

INTRODUCTION: The Inpatient Prospective Payment System, the framework for categorization of admissions, is based upon physician documentation leading to International Classification of Diseases, Tenth Revision code generation and Medical Severity Diagnosis-Related Group (MS-DRG) assignment. In this curriculum, we introduced internal medicine residents to this inpatient coding framework and its effects on hospital quality metrics and reimbursement. We focused on educating learners about the importance of physicians being proficient in providing thorough and specific clinical documentation to produce appropriate DRG assignment.

METHODS: Internal medicine residents participated in a 90-minute session that introduced the basic framework of inpatient coding, discussed effects of …

Addressing Maternal Mental Health Among Black Perinatal Women In Atlanta, Georgia: A Cbpr Approach, Natalie Hernandez, Sherilyn Francis, Brielle Evans, Andrea G. Parker, Jemea Dorsey, Danette Mclaurin Glass, Mica Whitfield, Elise Blasingame, Pamela Braxton, Rasheeta Chandler

Journal of the Georgia Public Health Association

Background: Perinatal mood and anxiety disorders (PMADs) are the most common complication of pregnancy, affecting up to 1 in 5 childbearing women, with higher rates among low-income minority women. One in seven women suffer from perinatal depression. This study aimed to (1) assess the mental and emotional health challenges of underserved Black perinatal women in Atlanta, Georgia (GA) and (2) examine local priorities and sources of care for mental health among underserved women in Atlanta, GA.

Methods: A community-based participatory research partnership between Morehouse School of Medicine (MSM) and the Center for Black Women’s Wellness (CBWW)’s Atlanta Healthy Start Community …

Il-17 Producing Lymphocytes Cause Dry Eye And Corneal Disease With Aging In Rxrα Mutant Mouse, Jehan Alam, Ghasem Yazdanpanah, Rinki Ratnapriya, Nicholas Borcherding, Cintia S De Paiva, Dequan Li, Rodrigo Guimaraes De Souza, Zhiyuan Yu, Stephen C Pflugfelder

Faculty, Staff and Students Publications

PURPOSE: To investigate IL-17 related mechanisms for developing dry eye disease in the Pinkie mouse strain with a loss of function RXRα mutation.

METHODS: Measures of dry eye disease were assessed in the cornea and conjunctiva. Expression profiling was performed by single-cell RNA sequencing (scRNA-seq) to compare gene expression in conjunctival immune cells. Conjunctival immune cells were immunophenotyped by flow cytometry and confocal microscopy. The activity of RXRα ligand 9-cis retinoic acid (RA) was evaluated in cultured monocytes and γδ T cells.

RESULTS: Compared to wild type (WT) C57BL/6, Pinkie has increased signs of dry eye disease, including decreased tear …

Subjective Versus Objective Tests Of Dizziness And Vestibular Function In Epidemiologic Screening Research, Helen S Cohen, Haleh Sangi-Haghpeykar, Michael W Plankey

Faculty, Staff and Students Publications

BACKGROUND: Many epidemiologic studies of vestibular disorders are based on responses to questionnaires which have not been tested against objective tests of the vestibular system.

OBJECTIVE: The goal was to determine if the dizziness and balance questions used in the National Health Interview Survey (NHIS) are valid and predict performance on objective tests of the vestibular system (VNG).

METHODS: Data from 367 participants recruited from the community, aged 21.4 to 87.6 years, were collected in the Otolaryngology department at a tertiary care center. They were asked the eight NHIS questions twice, at least 30 minutes apart and were tested on …

Comparison Of The Different Isoforms Of Vitamin E Against Amyloid Beta-Induced Neurodegeneration, Alp Yi̇ği̇t Özdemi̇r, Esi̇n Çeti̇n, Mehmet Ali̇ Onur

Turkish Journal of Biology

Neurodegeneration is the progressive loss of structure or function of neurons. Amyloid beta oligomers and aggregates have been linked to neurodegeneration. While previous studies have suggested that dietary α-tocopherol intake can prevent amyloid beta aggregation and protect the brain against neurotoxicity, other research, however, indicated that tocotrienol forms might be used as an alternate agent against this kind of degeneration. In the presented research, we compared the in vitro protective effects of α-tocopherol and α-tocotrienol. In this context, we formed an in vitro neurodegeneration model with primary isolated neurons and measured α-tocopherol's and α-tocotrienol's protective effects. As a result, α-tocopherol …

Hypermethylation Of Pi3k-Akt Signalling Pathway Genes Is Associated With Human Neural Tube Defects, Tian Tian, Xinyuan Lai, Kuanhui Xiang, Xiao Han, Shengju Yin, Robert M Cabrera, John W Steele, Yunping Lei, Xuanye Cao, Richard H Finnell, Linlin Wang, Aiguo Ren

Faculty, Staff and Students Publications

Neural tube defects (NTDs) are a group of common and severe congenital malformations. The PI3K-AKT signalling pathway plays a crucial role in the neural tube development. There is limited evidence concerning any possible association between aberrant methylation in PI3K-AKT signalling pathway genes and NTDs. Therefore, we aimed to investigate potential associations between aberrant methylation of PI3K-AKT pathway genes and NTDs. Methylation studies of PI3K-AKT pathway genes utilizing microarray genome-methylation data derived from neural tissues of ten NTD cases and eight non-malformed controls were performed. Targeted DNA methylation analysis was subsequently performed in an independent cohort of 73 NTD cases and …

Cytomegalovirus Infection Among Patients With Cancer Receiving Immune Checkpoint Inhibitors, Kavea Panneerselvam, David Szafron, Rajan N Amin, Dongguang Wei, Dongfeng Tan, Mehmet Altan, Pablo C Okhuysen, Malek Shatila, Gottumukkala Subba Raju, Anusha S Thomas, Yinghong Wang

Faculty, Staff and Students Publications

BACKGROUND: Immune checkpoint inhibitors (ICIs), used for the treatment of solid and hematologic malignancies, come with the risk of immune-related adverse events (irAEs). Opportunistic infections (e.g., cytomegalovirus [CMV]) mimic irAE symptoms and are understudied in this population. We aimed to describe the incidence, characteristics, treatment and outcomes of CMV infection in ICI-treated patients.

METHODS: We conducted a single-center retrospective review of all adult patients who were CMV-positive after ICI therapy between 06/2011 and 05/2020. A CMV-positive non-ICI cohort was matched to the ICI group based on age, sex and cancer type. Variables of interest were collected through electronic medical records. …

Feeling The Burn: Assessing The Effects Of Fire Severity On Elk Nutritional Resources And Resource Selection, Lauren Ashley Snobl

Graduate Student Theses, Dissertations, & Professional Papers

Wildfires in the western United States have been increasing in size and severity over the last several decades, and climate forecasts suggest a continued increase in wildfire activity in the future. Large-scale wildfires often burn in a mosaic-like pattern of varying fire severity creating a heterogenous landscape with concomitant effects on vegetation communities. By changing vegetative structure, fire severity potentially alters the availability and distribution of key resources for wildlife such as food and cover, ultimately influencing habitat use. However, the impact of fire on wildlife has frequently been treated as binary (i.e., burned vs unburned), and a deeper understanding …

Are Risk Attitudes And Rainfall Variability Determinants Of Diversification? Evidence From Rondonia, Brazil, Brenna Swinger

Graduate Student Theses, Dissertations, & Professional Papers

Risk exposure and management are inherent to smallholder farmers. One of the risks they face is climate change. Climate change decreases rainfall, increases frequency of drought, adds heat stress to crops from higher temperatures, and leads to higher rainfall variability. The threat of rainfall variability causes higher production in some years and lower in others. Even if the average income remains the same, people’s welfare is reduced by the variability and the size of the loss will depend on their attitudes towards risk.

Diversification is one adaption strategy that may help reduce climate risk. Diversified farmers produce multiple types of …

Keeping What You Sow: Intellectual Property Rights For Plant Breeders And Seed Growers, Paulina B. Jenney

Graduate Student Theses, Dissertations, & Professional Papers

Over the last 150 years, the food system in the present-day United States has undergone a transformational restructuring, from a diversified, decentralized, network of farmers and seed growers, to one in which the majority of crop production is controlled by a few industrial corporations. The consolidation of power has been under-girded by the application of intellectual property rights (IPR)—especially utility patents—to plant varieties and genetic traits, which are leveraged to exclude small-scale seed growers from accessing quality germplasm. Patents and restrictive licensing agreements recapitulate colonial structures by appropriating common and traditionally community-held resources for profit, and by creating reliance on …

Underwater: Science & Story In Aquatic Communities, Lena J. Beck

Graduate Student Theses, Dissertations, & Professional Papers

How do we manage fish species, and how do fish, in turn, impact us? This portfolio of three stories will explore some of the ways in which humans interact with aquatic landscapes. First, we’ll go to Flathead Lake in Montana, where invasive lake trout have all but decimated native trout species, and learn what the Confederated Salish and Kootenai Tribes are doing to address the issue. Then, we’ll examine stories of a legendary Big Fish in Alaska’s Lake Iliamna, and think about what it means to scientifically prove something as real. The last story looks at the aquaculture industry in …

Hunger On The Richest Hill: A Case Study Of Linked Historical And Contemporary Food Insecurity In Butte, Montana, Elizabeth Anne Bett Osborn

Graduate Student Theses, Dissertations, & Professional Papers

On the “Richest Hill on Earth” or Butte, Montana, a dilemma of food insecurity persists. This dilemma broadly consists of limited access to affordable, healthy, and appropriate nutrition for the city’s urban population. This thesis constructs a historically and contextually-informed understanding of food insecurity in Butte since the city’s establishment in 1864. Food insecurity research largely lacks place-specific consideration of historical events and processes which contribute to and reinforce the root factors contributing to food insecurity. Butte was once a thriving copper-mining boom town yet saw the decline of its primary industry in the late 20^th century. In 2020, …

Spatial Behavior, Foraging, And Population Dynamics Of A Subarctic Ungulate, Elizabeth (Libby) Parr Williamson Ehlers

Graduate Student Theses, Dissertations, & Professional Papers

The concept of density-dependence is foundational to our understanding of the ecology and management of large herbivores. As the abundance of large herbivores increases, competition for high-quality resources increases and animals select for lower-quality foods potentially resulting in reduced vital rates that cause declines in population growth. What remains unclear is how foraging at finer scales, like the ‘bite’, translates to demography. This is difficult because it’s challenging to scale bite-level foraging to population-levels. I test the fine-scale foraging mechanisms of density dependence in a large subarctic herbivore, Barrenground caribou (Rangifer tarandus granti). The nutritional landscape for arctic herbivores is …

The Mountain Home: Spatial And Optimal Foraging Assessments Of Hunter- Gatherer Mountain Landscape Use In The Beartooth Mountains, Montana, Scott William Dersam

Graduate Student Theses, Dissertations, & Professional Papers

Patterns of cultural mountain adaptations are recorded throughout the Western Cordillera of North America. They demonstrate prolonged and complex uses of these ecosystems over the Holocene. What behaviors or circumstances in the Early Holocene transitioned mountains from inhospitable to reliable territory? This research addresses this question in the Beartooth Mountains of the Greater Yellowstone Ecosystem (GYE).

Foraging behaviors dictate hunter-gatherer mobility and habitation patterning. These behaviors are reflected in the spatial arrangement of archaeological activity areas and lithic tools in mountainous ecosystems. Energy-demanding ecosystems, require foraging behaviors and habitation strategies that conserve energy. I assert that to understand the emergence …

Linear Programming Analysis And Diet Breadth Modeling At Bridge River, British Columbia, Sean Patrick Boyd

Graduate Student Theses, Dissertations, & Professional Papers

Studies in diet breadth modeling and patch choice have been and continue to be a hot topic of interest among practitioners of human behavioral ecology and the set of data available at Bridge River can certainly add to these debates and discussions that have been dominating anthropology in the past few decades. The faunal assemblage of Housepit 54’s 17 anthropogenic floors have provided researchers with a plethora of data that clearly indicates periods of resource depletion and partial to full site abandonment. Using Linear Programming and Diet Breadth Modelling I analyze the most represented species in the record and establish …

Juvenile Temperature Regulation In Apis Mellifera (Honey Bee) And The Impacts Of Brood Temperature Requirements On The Colony, Scott Debnam

Graduate Student Theses, Dissertations, & Professional Papers

Little is known about the energetic costs to insects of raising young. Honey bees collectively raise young, or brood, through a series of complex behaviors that appear to accelerate and synchronize the timing of brood maturation. These include maintaining the brood nest at warmer and consistent temperatures and the exceptional activity of "heater bees." The temperature at which juvenile insects are raised can profoundly affect their development. Apis mellifera (Honey bees) cope with temperature-dependent development via social behavior that maintains the relatively high and constant temperatures within the nest where the brood are raised. Yet juvenile honey bee development is …

Factors That Affect The Rate Of Preterm Birth: An Examination Of The Inter-Related Impacts Of Social Determinants, Behavior And Physical Health Status, Krystal Michelle Hunter

Dissertations

The work seeks to examine the factors that have significant relationships to the rate of preterm birth (PTB) along with its cost to society.

There are four papers within this work. This purpose of the first paper is to measure the impact that healthy behaviors have on the rate of PTB when modeled with other factors like household demographics, community deprivation, chronic disease and mental health. This work finds that positive health behaviors has a negative relationship with PTB. The interaction between body mass index (BMI) and social class and its relationship to PTB is examined in the second paper. …