Digital Commons Network^™

Effects Of Growth Factor Deprivation On Mkn-45 Spheroid Cells, Özlem Türksoy, Gökhan Terzi̇oğlu

Turkish Journal of Biology

Background/aim: Serum and growth factor deprivation, a common cellular stressor in solid tumors, arises upon irradiation, chemotherapy, and antiangiogenesis. Spheroid body culture aims to enrich cancer stem cells by using low attachment conditions and some growth factors, such as basic fibroblast growth factor and epidermal growth factor to support the spheroid formation in serum-free spheroid culture. However, spheroid culture without any growth factors can imitate the tumor environment more realistically. In this study, we aimed to identify the effect of growth factor deprivation on the MKN-45 gastric cancer cell line in terms of stemness characteristics. Materials and methods: The spheroids …

Beyond The Business Case: Moving From Transactional To Transformational Inclusion, Jamillah Bowman Williams

Georgetown Law Faculty Publications and Other Works

While workplace diversity is a hot topic, the extent to which the diversity management movement has effectively improved intergroup relations and reduced racial inequality remains unclear. Despite large investments in diversity and inclusion training and other company wide initiatives, historically excluded groups remain vastly underrepresented in leadership and the most lucrative careers, such as finance, law, and technology. This calls the efficacy of diversity, equity, and inclusion (DEI) efforts into question, particularly with respect to reducing racial inequality in the workplace.

This Article explains why it is time for organizational leaders to move beyond the transactional case for diversity and …

Advancing Racial Justice Through Civil And Criminal Academic Medical-Legal Partnerships, Yael Cannon, Vida Johnson

Georgetown Law Faculty Publications and Other Works

The medical-legal partnership (MLP) model, which brings attorneys and healthcare partners together to remove legal barriers to health, is a growing approach to addressing unmet civil legal needs. But MLPs are less prevalent in criminal defense settings, where they also have the potential to advance both health and legal justice. In fact, grave racial health inequities are deeply intertwined with both civil and criminal injustice. In both spheres, health justice is racial justice. Building on the experiences of the authors in their respective civil and criminal law school clinics at Georgetown University in Washington, D.C., this Article argues that academic …

Fungicide Sensitivity In P. Tritici-Repentis Diverse Population And Phenotyping Of Spelt Wheat For Multiple Diseases, Zunera Shabbir

Electronic Theses and Dissertations

Pyraclostrobin, a QoI fungicide, is extensively used in the management of fungal leaf spot diseases. In this study, 215 P. tritici-repentis isolates collected from wheat fields in South Dakota were evaluated for their sensitivity to Pyraclostrobin. Of 215 isolates, 48 isolates (22%) exhibited insensitivity based on G143A mutation and EC50 values using spore germination assay. Our results indicate reduced sensitivity to insensitivity to pyraclostrobin in some isolates suggesting regular monitoring of the P. tritici-repentis population to QoI fungicides is essential to track the evolution of insensitive population. Spelt wheat (Triticum spelta L.) is an ancient cereal that is gaining small …

Effects Of Dietary Genistein And Fugacity Modelling In An Aquaculture System, Travis Schaeffer

Electronic Theses and Dissertations

Fish production from aquaculture has continued to grow over the past several decades, with finfishes being fed formulated, fish meal-based diets supporting most of that industry’s growth. However, increased demand and costs, coupled with static volume and erratic supplies of fish meal, has resulted in the use of alternative plant-derived protein sources. A common oilseed protein source is soybean meal (SBM), because of its protein content and amino acid profile; however, it also contains multiple anti-nutritional factors, including phytoestrogens. Phytoestrogens are naturally produced, estrogenmimicking compounds, with isoflavones receiving the most investigation as higher levels of these compounds occur within SBM. …

Development Of Corn Kernel-Based Biocomposite Films For Food Packaging Applications, Swastika Bera

Electronic Theses and Dissertations

Most of the current and active food packaging resources and methods are nonbiodegradable and nonrenewable therefore harmful to the environment. Due to this, alternate sources of food packaging materials are in high demand. In this study, a bio-composite film has been developed, with Corn kernel powder as fiber reinforcement which is mixed with gelatin, and lignin two biopolymers as the matrix. The effect of Corn Kernel (CK) reinforcement on the Gelatin/Lignin (G/L) matrix on mechanical and barrier properties has been studied. CK has shown great potential as reinforcement to natural polymer, gelatin, and lignin (G/L) for food packaging applications as …

Effect Of Lighter (273 Kg) And Heavier (356 Kg) Initial Weight On Growth Performance Of Single-Source, Pre-Conditioned Beef Steers Fed A Single Growing-Finishing Diet, Thomas C. Norman

Electronic Theses and Dissertations

The objective of the study was to determine the influence that initial BW has on growth performance responses, efficiency of dietary net energy (NE) utilization, and carcass traits in feedlot steers. Light- and heavy-weight Charolais×Red Angus steers (n = 70) selected from a larger single-source group were used in a 209-d growing-finishing feedlot experiment at the Ruminant Nutrition Center, in Brookings, SD. On d-0 and d-1 weight and hip height (HH) measurements were collected for allotment purposes; the initial experimental weight was the average between d 0 and d 1 BW. Steers were assigned to two groups based on initial …

Indigenous Toxin Affects Cell Viability, While An Artificial Proteolytic Queueing Causes The Upregulation Of Specific Genes, Mohammed Salahuddin

Electronic Theses and Dissertations

Studying bacterial physiology is crucial to understand the fundamental mechanisms that govern bacterial growth, survival, and adaptation. This thesis combines two chapters investigating bacterial physiology by studying important cellular processes like bacterial toxin-antitoxin systems and proteolytic pathways. The first chapter involves studying bacterial toxin-antitoxin systems to understand the regulation and function of hypothetical toxin-antitoxin (TA) systems of bacteria and the effects of these TA systems on bacterial growth and survival. The emergence of these genetic modules in bacterial research and the unrevealing of some of their important roles in cell physiology in recent years has drawn much attention in scientific …

Diet And Management Strategies To Mitigate Decreased Feed Intake Associated With Terminal Implant Administration In Finishing Beef Steers, Alexandria M. Kelly

Electronic Theses and Dissertations

Terminal implant administration in beef steers often leads to decreased feed intake, leading to less growth. Our objective was to identify diet and management changes that can mitigate this decrease in feed intake. Single-sourced Angus x Simmental steers (n=27, initial BW= 385.1 ± 30.8 kg) were utilized in a completely randomized trial to assess the effects of animal movement and increased forage inclusion on rumination and feed intake after re-implanting. Steers were initially implanted with Synovex Choice (100 mg trenbolone acetate and 14 mg estradiol benzoate; Zoetis, Parsippany, NJ), then reimplanted 88 d later with Synovex Plus (200 mg trenbolone …

Evaluation Of Welded And Polished Stainless-Steel Surfaces In Supporting Sporeformer Biofilms In Dairy Plants, Taghreed Almalki

Electronic Theses and Dissertations

No abstract provided.

Evaluating Precision Supplementation Technology And Mathematical Nutrition Models For The Development Of Heifers Grazing Dormant Rangeland In Western South Dakota, Anna Katherine Dagel

Electronic Theses and Dissertations

The Great Plains supports about 40-50% of beef cattle production in the United States. Consequently, the beef cow-calf sector provides a large economic impact for states located within the Northern Great Plains (South Dakota Department of Agriculture, 2021). Producing profitable rangeland beef cattle through an economically efficient cowcalf operation presents several challenges. These challenges include providing adequate nutrition to meet the needs of growing heifers on rangelands, which requires additional labor and feed/supplement to achieve desired rates of gain and reach a target body weight, resulting in reproductive success. Implementation of precision livestock technology (PLT) and the use of precision …

Impact Of Covid-19 Relief Packages On U.S. Household Food Security And Food Expenditure, Bridget Yeboah Bafowaa

Electronic Theses and Dissertations

The COVID-19 pandemic led to changes in consumer shopping habits, including food expenditures. The pandemic induced changes in food expenditure patterns, food accessibility, and declining household income contributed to increased food insecurity during the pandemic. The objective of this study is to examine the impact of COVID-19- related relief packages on food security and food expenditures. The effect of Economic Impact Payments, P-EBT, SNAP, and free food will be considered. Using data from the Household Pulse Survey (HPS), a total sample of 1,899,929 households were analyzed in this study. The HPS is a nationally representative survey structured to produce data …

Rumen-Protected Methionine Supplementation Improves Lactation, Oxidative Status, And Immune Response During A Subclinical Mastitis Challenge In Lactating Dairy Cows., Anita Paz Lopez

Electronic Theses and Dissertations

Mastitis is a significant problem in the dairy industry, posing numerous challenges for dairy farmers worldwide. Mastitis refers to the inflammation of the mammary gland, primarily caused by bacterial infections. This condition affects the health and well-being of dairy cows and has severe economic implications for farmers and overall dairy production. The prevalence of mastitis substantially threatens milk quality, productivity, and profitability, making it a top concern in the dairy industry. Preventing mastitis in cows is crucial for maintaining the herd's health and ensuring highquality milk production. Nutritional strategies have recently gained greater attention due to a direct impact on …

Characterizing Vaginal Gene Expression In Prepubertal Gilts, Shannon Lee Dierking

Electronic Theses and Dissertations

The best-known predictor of reproductive success in gilts is the age at puberty. Early puberty (i.e., d170 – d180 of age) is associated with improved long-term reproductive performance and more full value offspring for market. The ability to predict, in the prepubertal stage, the age that a replacement gilt would be expected to achieve puberty has great reproductive and economic advantages. This work focuses on identifying biological markers that are potentially predictive of age at puberty. Study 1 identified changes in the vaginal epithelium during gilt reproductive development. Pre-pubertal gilts (n =13) were followed from d70 of age until first …

Exploring Disparities In Prenatal Smoking And The Role Of Adverse Childhood Experiences, Patricia Da Rosa

Electronic Theses and Dissertations

Cigarette smoking is the most common cause of premature death, killing half of its users. During pregnancy, smoking continues to be the leading cause of premature death for both mothers and newborns. Research has consistently demonstrated smoking is not randomly distributed across populations; it is, instead, socially patterned. Women from lower socioeconomic backgrounds, those experiencing mental health issues, and rural area residents are disproportionately affected. The life course framework emphasizes the impact of exposures in early life on shaping health inequities. Eliminating health inequities, including risky behaviors such as smoking, requires an understanding of the pathways that generate these inequities. …

The Effects Of Residue Amounts And Variety Selection On The Development Of Wheat Fungal Pathogens In South Dakota, Abrielle Tembreull

Electronic Theses and Dissertations

Roughly 50% of South Dakota cropland is under the practice of no-till, with large increases in acres of no-till in the last 20 or so years. No-till is beneficial for Great Plains states, such as South Dakota, as soil residues are withheld. Residues are shown to increase soil health through retention of moisture and increase of microorganisms, but residues can serve as a source of inoculum for fungal pathogens that can overwinter on them. As wheat is one of the most important food crops in the world, it is important to protect this vital crop from diseases. Diseases of wheat …

Effect Of Foliar And Preplant Sulfur Application On Soybean Nutrient Uptake, Yield, And Seed Protein And Oil Content, Gena Ram Mahato

Electronic Theses and Dissertations

In recent years, Sulfur (S) deficiency has been observed in soybean producing regions in the US and different parts of the world. The major factors behind the frequent occurrence of S deficiency are reduced atmospheric S deposition due to strict regulations on emissions from fossil fuels, large S removal from high yielding crops, less use of fertilizers containing S, and intensive cultivation practices. To test S limitations, I conducted two independent studies from 2019 to 2021 at two locations in Eastern South Dakota to determine the effect of foliar and preplant S application on soybean. The objectives of this research …

Ecological Impacts Of Anthropogenic Chemicals On Non-Target Species And The Role Of Plant Diversity In Ecosystem Restoration And Resilience, Shiva Torabian

Electronic Theses and Dissertations

Understanding ecosystem processes in rangelands is of paramount importance for ensuring global food security, given their pivotal role in sustaining forage and livestock production. Central to this understanding are fundamental processes such as nutrient cycling, decomposition, and plant biomass dynamics. However, the use of anthropogenic chemicals in agricultural practices has the potential to exert significant influence on these critical ecosystem functions. A pivotal factor in this context is the plant diversity inherent to these rangeland ecosystems. Plant diversity not only provides essential ecosystem functions but also enhances them with the capacity to withstand and recover from perturbations. Ecosystems characterized by …

Biological Control Of Bacterial Leaf Streak Of Wheat Using Antagonistic Rhizobacteria, Ashley Frederickson

Electronic Theses and Dissertations

Wheat (Triticum aestivum L.) is one of the world’s most valuable crops, and the primary food grain produced in the United States. A major disease in wheat production is Bacterial Leaf Streak (BLS) caused by the gram-negative bacterium Xanthomonas translucens pv. undulosa (Xtu). Very few management strategies are available to growers to manage this disease, as common chemicals do not provide adequate control. A number of studies have been conducted on biological control using Rhizospheric bacteria for the control of various plant diseases, but limited data is available on the efficacy of biological control using Rhizospheric bacteria for controlling Bacterial …

Implementation Of A Food Science And Nutrition Module In A Dental Undergraduate Curriculum, Michael Crowe, Michael O'Sullivan, Lewis Winning, Oscar Cassetti, Brian O'Connell, Aifric O'Sullivan, Eileen Gibney, Suzanne Doyle, Annemarie Bennett, Paula Moynihan

Articles

Although it is generally acknowledged that nutrition should be a core subject in curricula, most dental programmes do not specifically include nutrition education.1–5 The rationale for improving the nutrition education of dental students is supported by the multidirectional and synergistic relationship between nutrition and oral health.6,7 As highlighted recently, achieving a population-wide reduction in free sugar consumption has now become a central tenet for public health authorities and professional healthcare workers globally.8 However, dentists do not have the confidence or competencies to assess or deliver appropriate dietary interventions.

Silver(I) 1,10-Phenanthroline Complexes Are Active Against Fonsecaea Pedrosoi Viability And Negatively Modulate Its Potential Virulence Attributes, Ingrid S. Sousa Ingrid S. Sousa, Tatiana D.P. Vieira, Rubem F. S. Menna-Barreto, Allan J. Guimarães, Pauraic Mccarron, Malachy Mccann, Michael Devereux, André L. S. Santos, Lucimar F. Kneipp

Articles

The genus Fonsecaea is one of the etiological agents of chromoblastomycosis (CBM), a chronic subcutaneous disease that is difficult to treat. This work aimed to evaluate the effects of copper(II), manganese(II) and silver(I) complexes coordinated with 1,10-phenanthroline (phen)/1,10- phenanthroline-5,6-dione (phendione) on Fonsecaea spp. Our results revealed that most of these complexes were able to inhibit F. pedrosoi, F. monophora and F. nubica conidial viability with minimum inhibitory concentration (MIC) values ranging from 0.6 to 100 M. The most effective complexes against F. pedrosoi planktonic conidial cells, the main etiologic agent of CBM, were [Ag(phen)2]ClO4 and [Ag2(3,6,9-tdda)(phen)4].EtOH, (tdda: 3,6,9-trioxaundecanedioate), displaying MIC …

Peptidases Are Potential Targets Of Copper(Ii)-1,10-Phenanthroline-5,6-Dione Complex, A Promising And Potent New Drug Against Trichomonas Vaginalis, Graziela Vargas Rigo, Fernando Gomes Cardoso, Matheus Menonca Pereira, Michael Devereux, Malachy Mccann, Andre L.S. Santos, Tiana Tasca

Articles

Trichomonas vaginalis is responsible for 156 million new cases per year worldwide. When present asymptomatically, the parasite can lead to serious complications, such as development of cervical and prostate cancer. As infection increases the acquisition and transmission of HIV, the control of trichomoniasis represents an important niche for the discovery and development of new antiparasitic molecules. This urogenital parasite synthesizes several molecules that allow the establishment and pathogenesis of infection. Among them, peptidases occupy key roles as virulence factors, and the inhibition of these enzymes has become an important mechanism for modulating pathogenesis. Based on these premises, our group recently …

The Iris Clinic: A Protocol For A Mixed-Methods Study Evaluating The Management Of Hyperemesis Gravidarum, Eileen O'Brien, Jean Doherty, Sarah Louise Killeen, Melanie Bennett, Lillian Murtagh, Sinead Curran, Suzanne Murphy, Helen Mchale, Lucille Sheehy

Articles

Hyperemesis Gravidarum (HG) is a severe form of nausea and vomiting in pregnancy that affects 0.3–3% of women and has profound nutritional, physical and psychological consequences. Research is lacking regarding the most effective management of the condition. In response to patient feedback, a multidisciplinary HG day-case service (IRIS Clinic) was launched in 2020 at The National Maternity Hospital, Ireland. The clinic provides routine, day-case care in a comfortable space with pre-booked appointments. The MDT involves midwives, dietitians, perinatal mental health, obstetrics and pharmacy, and the nature of the clinic enables peer-to-peer support. As this clinic is the first of its …

The Impact Of Climate Change On The Cultural Identity Of Indigenous Peoples And The Nation’S First “Climate Refugees”, Jordan K. Medaris

American Indian Law Review

No abstract provided.

Colorectal Surveillance Outcomes From An Institutional Longitudinal Cohort Of Lynch Syndrome Carriers, Gabriel Del Carmen, Laura Reyes-Uribe, Daniel Goyco, Kyera Evans, Charles M Bowen, Jennifer L Kinnison, Valerie O Sepeda, Diane M Weber, Julie Moskowitz, Maureen E Mork, Selvi Thirumurthi, Patrick M Lynch, Miguel A Rodriguez-Bigas, Melissa W Taggart, Y Nancy You, Eduardo Vilar

Faculty, Staff and Student Publications

OBJECTIVE: Lynch Syndrome (LS) carriers have a significantly increased risk of developing colorectal cancer (CRC) during their lifetimes. Further stratification of this patient population may help in identifying additional risk factors that predispose to colorectal carcinogenesis. In most LS patients CRC may arise from adenomas, although an alternative non-polypoid carcinogenesis pathway has been proposed for

DESIGN: We collected demographics, genetic, colonoscopy, and pathology results from a total of 163 LS carriers who obtained regular screening care at MD Anderson Cancer Center. Data were extracted from the electronic health records into a REDCap database for analysis. Logistic regressions were performed to …

Association Between Occlusal Supports And Nutritional Status In Older Adults: A Systematic Review, Koji Mizutani, Risako Mikami, Yuriko Komagamine, Norio Aoyama, Yoshiyuki Sasaki, Takanori Iwata, Shunsuke Minakuchi, Manabu Kanazawa

Journal of Dental Sciences

Abstract Background/purpose Impaired masticatory performance results in nutritional deficiencies in older adults. This systematic review aims to investigate the following clinical question (CQ): Do occlusal supports impact nutritional intake or nutritional status in older individuals? Materials and methods An extensive systematic literature search was performed to summarize the currently available knowledge to address the CQ. The cohort and intervention studies with participants of ≧60 years old or a mean age of 65 years performed before May 2021 were included. Studies were required to measure the parameters related to occluding tooth pairs/occlusal units and food/nutrient intake and/or nutritional status. Bias risk …

Pregnancy Advance Directives, Joan H. Krause

Faculty Publications

Part I of this Article provides a general introduction to the various types of advance directives available in the United States, including their goals and limitations. Part II provides a detailed overview of pregnancy restrictions, including comparisons of the substantive restrictions, procedural issues, and rationales for restricting the application of advance directives during pregnancy. Part III offers a critical analysis of both the scholarship addressing pregnancy restrictions and the litigation seeking to challenge the restrictions, demonstrating that the existing legal framework has not been satisfactory in resolving the issues—a situation that will only be exacerbated by the Supreme Court’s recent …

Sorting Bugs And Features Of Mass Tort Bankruptcy, Melissa B. Jacoby

Faculty Publications

In 1997, after two years of study, the National Bankruptcy Review Commission recognized bankruptcy’s potential as a forum to address mass tort problems. It emphasized, however, that Congress needed to do much more to ensure due process, address major risks of under-compensation and inconsistent compensation, and reduce uncertainty about the lawfulness of these cases. United States Supreme Court cases invalidating particular limited-fund class actions raised the stakes, casting doubt on certain analogous bankruptcy practices.

Congress adopted neither the Commission’s proposals nor others on the topic. Mass tort bankruptcy practice continued without legislative clarifications or improvements, culminating in recent high-profile and …

Physicians Spreading Medical Misinformation: The Uneasy Case For Regulation, Richard S. Saver

Faculty Publications

Physicians have played a surprisingly prominent role in the current “infodemic” of false and misleading medical claims. Yet, state medical boards, the governmental agencies responsible for professional licensure and oversight, have sanctioned remarkably few physicians. Pushing back against the widespread criticism of medical boards for insufficient action, this Article questions the overall suitability of licensure regulation to police medical misinformation. First, uncertainty exists about medical boards’ jurisdiction and legal authority. Many misinformation claims have involved physicians communicating publicly, not while treating patients. Given the primarily patient-centered legal and ethical frameworks governing the practice of medicine, serious challenges arise in making …

An Examination Of Kinanthropometric And Physical Injury Risk Factors In Elite Australian Football, Callum J. Mccaskie

Theses: Doctorates and Masters

Kinanthropometric assessment is an integral part of understanding an athlete’s physical profile and readiness for competition. This typically includes a variety of different assessments which serve to quantify body shape, proportion, and composition in a bid to understand human physiology. Common assessments include stature, body mass, limb ratios, body circumferences, body mass index, skinfold testing, body composition testing, and musculoskeletal morphological evaluations. Specifically, kinanthropometric evaluations have involved the characterisation of athletes according to sport, sex, competition level and playing position. While this has provided researchers and practitioners with greater insight into athletic phenotypes, more purposeful kinanthropometric assessments which focus on …