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Exploring The Differences Of Gender On Physician Burnout And Perceptions Of Work Environment, Holly C. Adams Jun 2025

Exploring The Differences Of Gender On Physician Burnout And Perceptions Of Work Environment, Holly C. Adams

Doctoral Dissertations and Projects

Physician burnout is a growing public health crisis, threatening care quality, patient safety, and workforce sustainability. Research suggests gender may influence burnout levels and perceptions of the work environment. This quantitative, cross-sectional study examined gender differences in physician burnout and workplace perceptions among U.S. physicians that impact burnout. A total of 170 licensed physicians completed an online survey using the Maslach Burnout Inventory-Human Services Survey (MBI-HSS (MP)) and the Areas of Worklife Survey (AWS). MANOVA results indicated a significant multivariate effect of gender on burnout. Follow-up ANOVA testing revealed that gender was significantly related to emotional exhaustion and personal accomplishment. …


Vitamin D And Cognition: Demographic Disparities In Memory Recall And Word Intrusion In A Multiethnic Cohort, Juan Lopez-Alvarenga, Isabel Omaña-Guzmán, Oscar Rosas-Carrasco, Jose E. Cavazos, Michael C. Mahaney, Gladys E. Maestre Jun 2025

Vitamin D And Cognition: Demographic Disparities In Memory Recall And Word Intrusion In A Multiethnic Cohort, Juan Lopez-Alvarenga, Isabel Omaña-Guzmán, Oscar Rosas-Carrasco, Jose E. Cavazos, Michael C. Mahaney, Gladys E. Maestre

School of Medicine Publications

Background

Vitamin D3 is essential for calcium metabolism and exerts pleiotropic effects, including neuroprotective activities in cognition. Its insufficiency has been linked to dementia, Alzheimer's disease, and cognitive impairments. The association between vitamin D3 and particular cognitive functions, including memory recall and word intrusion, remains imprecise, particularly among diverse ethnic and socioeconomic groups.

Objective

To examine the relationship between vitamin D3 levels with memory recall and word intrusion in individuals aged 60 and above, emphasizing demographic differences.

Methods

Data was collected from 2759 individuals in the NHANES 2011–2014 surveys. Cognitive performance was evaluated with the CERAD Word Learning, Animal Fluency, …


Eng 1002g-600 College Composition Ii Argument & Critical Inquiry, Melissa Ames Jun 2025

Eng 1002g-600 College Composition Ii Argument & Critical Inquiry, Melissa Ames

Summer 2025

No abstract provided.


Mechanisms Of The Quality Changes Of Polygonati Rhizoma Processed By Steaming-Drying Cycles, Zicong Yu, Liuping Fan Jun 2025

Mechanisms Of The Quality Changes Of Polygonati Rhizoma Processed By Steaming-Drying Cycles, Zicong Yu, Liuping Fan

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the effects of steaming-drying cycles on the quality changes of Polygonati rhizoma and optimize the processing technology to enhance its application value.[Method ] Slices of Polygonati rhizoma processed by 0~5 steaming-drying cycles were prepared.The drying rate and drying kinetics during the drying stage after each cycle were analyzed,and a drying model was fitted.Changes in color difference,appearance,hardness,and mass fractions of polysaccharides,reducing sugars,polyphenols,flavonoids,saponins,and calcium oxalate raphides were measured.[Result ] The number of steaming-drying cycles significantly affected the quality of Polygonati rhizoma.The drying rate decreased as the drying time increased,conforming to the Modified Page model,while the …


Safety Properties , Growth Characteristics , And Fermentation Performance Of Tetragenococcus Halophilus, Ranhui Lyu, Yanhua Zhong, Yang You, Runhan Yuan, Enyi Guan, Shan Duan Jun 2025

Safety Properties , Growth Characteristics , And Fermentation Performance Of Tetragenococcus Halophilus, Ranhui Lyu, Yanhua Zhong, Yang You, Runhan Yuan, Enyi Guan, Shan Duan

Journal of Food Science and Biotechnology

[Objective ] The physiological characteristics vary greatly among different strains of Tetragenococcus halophilus (T.halophilus ).By comparing some indicators of safety properties,growth characteristics,and fermentation performance of T.halophilus strains from different sources,the authors aim to establish a regular understanding of this bacterial species.[Method ] Seventeen strains of T.halophilus derived from different aquatic products and soy sauce mash were selected,and their antibiotic resistance,biogenic amine production,hemolytic activity,growth under different environmental conditions,and flavor characteristics after fermenting soybean hydrolysate were determined.Additionally,correlation analysis was conducted to identify the associations among various indicators.[Result ] In terms of safety properties,the 17 strains were generally sensitive to penicillin,vancomycin,and tetracycline,but resistant …


Construction , Structural Characterization , And Antioxidant Activity Evaluation Of Yam Polysaccharide-Selenium Nanoparticle Complexes, Yidong Xiao, Lanmei Ou, Mengmeng Zhao Jun 2025

Construction , Structural Characterization , And Antioxidant Activity Evaluation Of Yam Polysaccharide-Selenium Nanoparticle Complexes, Yidong Xiao, Lanmei Ou, Mengmeng Zhao

Journal of Food Science and Biotechnology

[Objective ] This study explored the structural characteristics and in vitro antioxidant activity of yam polysaccharide-selenium nanoparticle (YP-SeNPs ) complexes.[Method ] Yam polysaccharide (YP) were obtained through hot water extraction and then purified by purification,diethylaminoethyl cellulose 52 (DEAE-52) column chromatography,and dialysis.With YP as a template,YP-SeNPs were prepared with different Se/YP mass ratios (1∶3,1∶1,and 4∶3) in an oxidation-reduction system comprising ascorbic acid (VC) and sodium selenite (Na 2SeO 3).The complexes were characterized via multiple techniques,including visual observation of solution color,ultraviolet-visible absorption (UV-vis ) spectroscopy,particle size measurement,transmission electron microscopy (TEM ),scanning electron microscopy (SEM ),atomic force microscopy (AFM ),Fourier-transform infrared spectroscopy (FT-IR …


Research Progress In The Application Of Tea Polyphenols In Meat Processing And Preservation, Yanting Gao, Huanhuan Li, Teng Xu, Ke Zhao, Mingwei Su, Liwen Wu, Honggang Tang Jun 2025

Research Progress In The Application Of Tea Polyphenols In Meat Processing And Preservation, Yanting Gao, Huanhuan Li, Teng Xu, Ke Zhao, Mingwei Su, Liwen Wu, Honggang Tang

Journal of Food Science and Biotechnology

Tea polyphenols are the general term for polyphenols in tea,mainly including catechins,flavonoids,and anthocyanins,which have antioxidant and antimicrobial properties.The author reviewed the structural characteristics and the antioxidant and antibacterial mechanisms of tea polyphenols.Additionally,the application of tea polyphenols in the processing and preservation of meat products is summarized,providing a theoretical basis for their use in meat products.Studies have shown that tea polyphenols can achieve anti-oxidation by scavenging free radicals,chelating metal ions,and inhibiting oxidase activity.Moreover,tea polyphenols can inhibit cell growth and proliferation by inhibiting the activities of enzymes involved in cell metabolism and damaging the cell membrane system.As natural antioxidants,tea polyphenols can significantly …


Safety Properties , Growth Characteristics , And Fermentation Performance Of Tetragenococcus Halophilus, Ranhui Lyu, Yanhua Zhong, Yang You, Runhan Yuan, Enyi Guan, Shan Duan Jun 2025

Safety Properties , Growth Characteristics , And Fermentation Performance Of Tetragenococcus Halophilus, Ranhui Lyu, Yanhua Zhong, Yang You, Runhan Yuan, Enyi Guan, Shan Duan

Journal of Food Science and Biotechnology

[Objective ] The physiological characteristics vary greatly among different strains of Tetragenococcus halophilus (T.halophilus ).By comparing some indicators of safety properties,growth characteristics,and fermentation performance of T.halophilus strains from different sources,the authors aim to establish a regular understanding of this bacterial species.[Method ] Seventeen strains of T.halophilus derived from different aquatic products and soy sauce mash were selected,and their antibiotic resistance,biogenic amine production,hemolytic activity,growth under different environmental conditions,and flavor characteristics after fermenting soybean hydrolysate were determined.Additionally,correlation analysis was conducted to identify the associations among various indicators.[Result ] In terms of safety properties,the 17 strains were generally sensitive to penicillin,vancomycin,and tetracycline,but resistant …


Construction , Structural Characterization , And Antioxidant Activity Evaluation Of Yam Polysaccharide-Selenium Nanoparticle Complexes, Yidong Xiao, Lanmei Ou, Mengmeng Zhao Jun 2025

Construction , Structural Characterization , And Antioxidant Activity Evaluation Of Yam Polysaccharide-Selenium Nanoparticle Complexes, Yidong Xiao, Lanmei Ou, Mengmeng Zhao

Journal of Food Science and Biotechnology

[Objective ] This study explored the structural characteristics and in vitro antioxidant activity of yam polysaccharide-selenium nanoparticle (YP-SeNPs ) complexes.[Method ] Yam polysaccharide (YP) were obtained through hot water extraction and then purified by purification,diethylaminoethyl cellulose 52 (DEAE-52) column chromatography,and dialysis.With YP as a template,YP-SeNPs were prepared with different Se/YP mass ratios (1∶3,1∶1,and 4∶3) in an oxidation-reduction system comprising ascorbic acid (VC) and sodium selenite (Na 2SeO 3).The complexes were characterized via multiple techniques,including visual observation of solution color,ultraviolet-visible absorption (UV-vis ) spectroscopy,particle size measurement,transmission electron microscopy (TEM ),scanning electron microscopy (SEM ),atomic force microscopy (AFM ),Fourier-transform infrared spectroscopy (FT-IR …


Preparation And Anti-Exercise Fatigue Effect Evaluation Of Γ-Aminobutyric Acid-Enriched Milk Fermented With Lactic Acid Bacteria, Dong Liu, Yuning Yang, Fangfei Dou, Yuxing Li, Minghai Ju Jun 2025

Preparation And Anti-Exercise Fatigue Effect Evaluation Of Γ-Aminobutyric Acid-Enriched Milk Fermented With Lactic Acid Bacteria, Dong Liu, Yuning Yang, Fangfei Dou, Yuxing Li, Minghai Ju

Journal of Food Science and Biotechnology

[Objective ] This study aims to prepare γ-aminobutyric acid (GABA )-enriched milk fermented with lactic acid bacteria and evaluate its anti-exercise fatigue effect.[Method ] High GABA-producing lactic acid bacteria were selected and used to ferment milk with a commercial starter culture.A mouse model of exercise fatigue was established by four weeks of exhaustive swimming training.After four weeks,the exhaustive swimming time,serum biochemical indicators,antioxidant indicators,and energy storage substance levels were measured and analyzed.[Result ] Lactobacillus plantarum (L.plantarum ) LP- 5 was selected as the strain for fermentation,and its GABA yield reached (1.340±0.003) g/L.The fermented milk showed the titratable acidity of (46.29±2.01) ºT,the …


Study On The Antioxidant Effects Of Natural Antioxidants And Their Derivatives On Anchovy (Engraulis Japonicus ) Oil, Xuyang Ding, Fujun Liu, Fawen Yin, Dayong Zhou Jun 2025

Study On The Antioxidant Effects Of Natural Antioxidants And Their Derivatives On Anchovy (Engraulis Japonicus ) Oil, Xuyang Ding, Fujun Liu, Fawen Yin, Dayong Zhou

Journal of Food Science and Biotechnology

[Objective ] The aim of this study is to evaluate and improve the antioxidant effects of natural antioxidants and their derivatives on anchovy (Engraulis japonicus ) oil.[Method ] The 60 ℃ accelerated oxidation model was used to evaluate the antioxidant effects of phytic acid,tea polyphenols (TP),rosemary extract,ascorbyl palmitate,vitamin E,tea polyphenol palmitate (TPP),and bamboo leaf extract on anchovy oil based on the peroxide value (POV ),thiobarbituric acid reactive substances (TBARS ) value,relative intensity of free radicals,and fatty acid composition.[Result ] TPP (single antioxidant ) and TPP+TP (composite antioxidant ) demonstrated the strongest antioxidant effects on anchovy oil.[Conclusion ] Although the antioxidant …


Research Progress In Application Of Ultra-High Pressure Sterilization In The Processing Of Prepared Dishes, Haisheng Dong, Peng Zang Jun 2025

Research Progress In Application Of Ultra-High Pressure Sterilization In The Processing Of Prepared Dishes, Haisheng Dong, Peng Zang

Journal of Food Science and Biotechnology

As a non-thermal sterilization technology,ultra-high pressure sterilization can protect the thermosensitive nutrients and maintain the texture of food,demonstrating unique technical advantages in the processing of prepared dishes.By a review of domestic and foreign literature and performance of relevant equipment,this paper introduced the principle,equipment,and parameters of ultra-high pressure sterilization.Considering the parameters of ultra-high pressure sterilization and the effects of food ingredients on the pressure tolerance of different microbial species,the factors affecting the effect of ultra-high pressure sterilization were analyzed.In addition,the effects of ultra-high pressure sterilization on the physical and chemical properties,sensory quality,enzyme activity,microorganisms count and bioactive substances of prepared dishes were …


Research Progress In The Application Of Tea Polyphenols In Meat Processing And Preservation, Yanting Gao, Huanhuan Li, Teng Xu, Ke Zhao, Mingwei Su, Liwen Wu, Honggang Tang Jun 2025

Research Progress In The Application Of Tea Polyphenols In Meat Processing And Preservation, Yanting Gao, Huanhuan Li, Teng Xu, Ke Zhao, Mingwei Su, Liwen Wu, Honggang Tang

Journal of Food Science and Biotechnology

Tea polyphenols are the general term for polyphenols in tea,mainly including catechins,flavonoids,and anthocyanins,which have antioxidant and antimicrobial properties.The author reviewed the structural characteristics and the antioxidant and antibacterial mechanisms of tea polyphenols.Additionally,the application of tea polyphenols in the processing and preservation of meat products is summarized,providing a theoretical basis for their use in meat products.Studies have shown that tea polyphenols can achieve anti-oxidation by scavenging free radicals,chelating metal ions,and inhibiting oxidase activity.Moreover,tea polyphenols can inhibit cell growth and proliferation by inhibiting the activities of enzymes involved in cell metabolism and damaging the cell membrane system.As natural antioxidants,tea polyphenols can significantly …


Determination Of Microorganisms And Analysis Of Factors Influencing Osmophilic Yeasts In Apis Cerana Honey, Mei Song, Hongxia Li, Zhaolong Liu, Dong Qiao, Na Li, Lanzhen Chen Jun 2025

Determination Of Microorganisms And Analysis Of Factors Influencing Osmophilic Yeasts In Apis Cerana Honey, Mei Song, Hongxia Li, Zhaolong Liu, Dong Qiao, Na Li, Lanzhen Chen

Journal of Food Science and Biotechnology

[Objective ] This study determined the mold count,total plate count,and osmophilic yeast count in Apis cerana honey and explored the correlations of osmophilic yeasts with quality parameters and storage conditions of the honey.[Method ] A total of 105 Apis cerana honey samples were used for the determination of mold count,total plate count,and osmophilic yeast count,as well as odor,moisture,phenolic acids,and carbohydrates.The correlations between quality indexes and osmophilic yeasts and the effects of different storage conditions on osmophilic yeast count were explored.[Result ] Among the 105 samples,1,1,and 67 samples showed the mold count,total plate count,and osmophilic yeast count exceeding the national standards.All …


Prokaryotic Expression , Purification , And Polyclonal Antibody Preparation Of Chlamydomonas Reinhardtii Channelrhodopsin 1, Jiurong Wang, Bin Xue, Zhenchuan Fan Jun 2025

Prokaryotic Expression , Purification , And Polyclonal Antibody Preparation Of Chlamydomonas Reinhardtii Channelrhodopsin 1, Jiurong Wang, Bin Xue, Zhenchuan Fan

Journal of Food Science and Biotechnology

[Objective ] This study aims to prepare a highly specific antibody against channelrhodopsin- 1 (ChR 1) from Chlamydomonas reinhardtii (C.reinhardtii ) and investigate its regulatory mechanisms in cellular phototaxis and negative phototaxis.[Method ] The recombinant expression vector pET- 28a(+)-chr1 was constructed and transformed into Escherichia coli (E.coli ) BL21 (DE3) competent cells.The expression of the fusion protein 6×His∷ChR 1 was induced with IPTG,and the obtained protein was purified by nickel-affinity chromatography.The purified protein was used to immunize New Zealand white rabbits for the production of ChR 1 antiserum.ChR 1-specific IgG antibodies were enriched from the antiserum through protein A purification.The …


Engineering Escherichia Coli Tolerance To Improve Succinic Acid Production, Jingyu Pan, Yongsheng Tang, Jia Liu, Cong Gao, Xiaomin Li, Wei Song, Wanqing Wei, Guipeng Hu, Jing Wu, Liming Liu, Xiulai Chen Jun 2025

Engineering Escherichia Coli Tolerance To Improve Succinic Acid Production, Jingyu Pan, Yongsheng Tang, Jia Liu, Cong Gao, Xiaomin Li, Wei Song, Wanqing Wei, Guipeng Hu, Jing Wu, Liming Liu, Xiulai Chen

Journal of Food Science and Biotechnology

[Objective ] This study aims to enhance the high osmotic pressure tolerance of Escherichia coli (E.coli ) for the efficient production of succinic acid.[Method ] Adaptive evolution,transcriptomic analysis,reverse metabolic engineering,and fermentation optimization were employed for improving E.coli tolerance to high osmotic pressure.[Result ] By analyzing the key factors influencing succinic acid production by fermentation,osmotic pressure was identified as the critical factor reducing succinic acid production efficiency.A mutant strain,E.coli FN-6,with tolerance to high osmotic pressure was obtained through adaptive evolution.The key gene cusC responsible for osmotic pressure tolerance was identified by transcriptomic analysis.After optimization of the expression level of cusC,the engineered …


Rapid Determination Of Vitamin B 1 In Infant Formula Milk Powder By Fluorescent Microsphere Immunochromatographic Assay, Qianqian Lu, Ting Yang, Yongwei Feng, Jialin Hu, Hua Kuang, Chuanlai Xu, Lingling Guo Jun 2025

Rapid Determination Of Vitamin B 1 In Infant Formula Milk Powder By Fluorescent Microsphere Immunochromatographic Assay, Qianqian Lu, Ting Yang, Yongwei Feng, Jialin Hu, Hua Kuang, Chuanlai Xu, Lingling Guo

Journal of Food Science and Biotechnology

[Objective ] A fluorescent immunochromatographic assay was established based on vitamin B 1 monoclonal antibody to measure the vitamin B 1 in infant formula milk powder.[Method ] The performance of vitamin B 1 monoclonal antibody was evaluated.On this basis,a highly sensitive fluorescent immunochromatographic test strip was developed.[Result ] The vitamin B 1 monoclonal antibody showed high sensitivity,affinity,and specificity.The fluorescent immunochromatographic test strip showed a visual limit of detection (vLOD ) of 10 μg/kg,a cut-off value of 200 μg/kg,and a calculated limit of detection (cLOD ) of 2.056 μg/kg.In addition,the recovery rate of this method was 97.3%~115.4%.The test results of the …


Preparation And Anti-Exercise Fatigue Effect Evaluation Of Γ-Aminobutyric Acid-Enriched Milk Fermented With Lactic Acid Bacteria, Dong Liu, Yuning Yang, Fangfei Dou, Yuxing Li, Minghai Ju Jun 2025

Preparation And Anti-Exercise Fatigue Effect Evaluation Of Γ-Aminobutyric Acid-Enriched Milk Fermented With Lactic Acid Bacteria, Dong Liu, Yuning Yang, Fangfei Dou, Yuxing Li, Minghai Ju

Journal of Food Science and Biotechnology

[Objective ] This study aims to prepare γ-aminobutyric acid (GABA )-enriched milk fermented with lactic acid bacteria and evaluate its anti-exercise fatigue effect.[Method ] High GABA-producing lactic acid bacteria were selected and used to ferment milk with a commercial starter culture.A mouse model of exercise fatigue was established by four weeks of exhaustive swimming training.After four weeks,the exhaustive swimming time,serum biochemical indicators,antioxidant indicators,and energy storage substance levels were measured and analyzed.[Result ] Lactobacillus plantarum (L.plantarum ) LP- 5 was selected as the strain for fermentation,and its GABA yield reached (1.340±0.003) g/L.The fermented milk showed the titratable acidity of (46.29±2.01) ºT,the …


Research Progress In Application Of Ultra-High Pressure Sterilization In The Processing Of Prepared Dishes, Haisheng Dong, Peng Zang Jun 2025

Research Progress In Application Of Ultra-High Pressure Sterilization In The Processing Of Prepared Dishes, Haisheng Dong, Peng Zang

Journal of Food Science and Biotechnology

As a non-thermal sterilization technology,ultra-high pressure sterilization can protect the thermosensitive nutrients and maintain the texture of food,demonstrating unique technical advantages in the processing of prepared dishes.By a review of domestic and foreign literature and performance of relevant equipment,this paper introduced the principle,equipment,and parameters of ultra-high pressure sterilization.Considering the parameters of ultra-high pressure sterilization and the effects of food ingredients on the pressure tolerance of different microbial species,the factors affecting the effect of ultra-high pressure sterilization were analyzed.In addition,the effects of ultra-high pressure sterilization on the physical and chemical properties,sensory quality,enzyme activity,microorganisms count and bioactive substances of prepared dishes were …


Physicochemical Indicators , Microbial Diversity , And Volatile Substances In The Qupi And Quxin Of High-Temperature Daqu, Dan Xie, Cheng Wu, Wenyu Zhao, Jianfeng Hu, Pingyan Cheng, Yuanlin Bi, Junlin Yang, Xiaolong You, Diqiang Wang Jun 2025

Physicochemical Indicators , Microbial Diversity , And Volatile Substances In The Qupi And Quxin Of High-Temperature Daqu, Dan Xie, Cheng Wu, Wenyu Zhao, Jianfeng Hu, Pingyan Cheng, Yuanlin Bi, Junlin Yang, Xiaolong You, Diqiang Wang

Journal of Food Science and Biotechnology

[Objective ] This study aims to explore the physicochemical indicators,microbial diversity,and volatile substances in the Qupi (surface ) and Quxin (core) of high-temperature Daqu.[Method ] High-throughput sequencing and headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS ) were employed to compare the microbial diversity,volatile substances,and physicochemical indicators between Qupi and Quxin of three types of Daqu.[Result ] The moisture mass fraction in Qupi of the three types of Daqu was lower than that in Quxin (P<0.05),and the moisture mass fraction was the lowest in the Qupi of white Daqu (WP) and the highest in the Quxin of yellow Daqu (YX).The acidity of Qupi and Quxin were in the order of Quxin of black Daqu (BX) > YX > Qupi of black Daqu (BP) > Quxin of white Daqu (WX) > Qupi of yellow Daqu (YP) > WP.High-throughput sequencing results showed that Firmicutes and …


Mechanisms Of The Quality Changes Of Polygonati Rhizoma Processed By Steaming-Drying Cycles, Zicong Yu, Liuping Fan Jun 2025

Mechanisms Of The Quality Changes Of Polygonati Rhizoma Processed By Steaming-Drying Cycles, Zicong Yu, Liuping Fan

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the effects of steaming-drying cycles on the quality changes of Polygonati rhizoma and optimize the processing technology to enhance its application value.[Method ] Slices of Polygonati rhizoma processed by 0~5 steaming-drying cycles were prepared.The drying rate and drying kinetics during the drying stage after each cycle were analyzed,and a drying model was fitted.Changes in color difference,appearance,hardness,and mass fractions of polysaccharides,reducing sugars,polyphenols,flavonoids,saponins,and calcium oxalate raphides were measured.[Result ] The number of steaming-drying cycles significantly affected the quality of Polygonati rhizoma.The drying rate decreased as the drying time increased,conforming to the Modified Page model,while the …


Determination Of Microorganisms And Analysis Of Factors Influencing Osmophilic Yeasts In Apis Cerana Honey, Mei Song, Hongxia Li, Zhaolong Liu, Dong Qiao, Na Li, Lanzhen Chen Jun 2025

Determination Of Microorganisms And Analysis Of Factors Influencing Osmophilic Yeasts In Apis Cerana Honey, Mei Song, Hongxia Li, Zhaolong Liu, Dong Qiao, Na Li, Lanzhen Chen

Journal of Food Science and Biotechnology

[Objective ] This study determined the mold count,total plate count,and osmophilic yeast count in Apis cerana honey and explored the correlations of osmophilic yeasts with quality parameters and storage conditions of the honey.[Method ] A total of 105 Apis cerana honey samples were used for the determination of mold count,total plate count,and osmophilic yeast count,as well as odor,moisture,phenolic acids,and carbohydrates.The correlations between quality indexes and osmophilic yeasts and the effects of different storage conditions on osmophilic yeast count were explored.[Result ] Among the 105 samples,1,1,and 67 samples showed the mold count,total plate count,and osmophilic yeast count exceeding the national standards.All …


Prokaryotic Expression , Purification , And Polyclonal Antibody Preparation Of Chlamydomonas Reinhardtii Channelrhodopsin 1, Jiurong Wang, Bin Xue, Zhenchuan Fan Jun 2025

Prokaryotic Expression , Purification , And Polyclonal Antibody Preparation Of Chlamydomonas Reinhardtii Channelrhodopsin 1, Jiurong Wang, Bin Xue, Zhenchuan Fan

Journal of Food Science and Biotechnology

[Objective ] This study aims to prepare a highly specific antibody against channelrhodopsin- 1 (ChR 1) from Chlamydomonas reinhardtii (C.reinhardtii ) and investigate its regulatory mechanisms in cellular phototaxis and negative phototaxis.[Method ] The recombinant expression vector pET- 28a(+)-chr1 was constructed and transformed into Escherichia coli (E.coli ) BL21 (DE3) competent cells.The expression of the fusion protein 6×His∷ChR 1 was induced with IPTG,and the obtained protein was purified by nickel-affinity chromatography.The purified protein was used to immunize New Zealand white rabbits for the production of ChR 1 antiserum.ChR 1-specific IgG antibodies were enriched from the antiserum through protein A purification.The …


Engineering Escherichia Coli Tolerance To Improve Succinic Acid Production, Jingyu Pan, Yongsheng Tang, Jia Liu, Cong Gao, Xiaomin Li, Wei Song, Wanqing Wei, Guipeng Hu, Jing Wu, Liming Liu, Xiulai Chen Jun 2025

Engineering Escherichia Coli Tolerance To Improve Succinic Acid Production, Jingyu Pan, Yongsheng Tang, Jia Liu, Cong Gao, Xiaomin Li, Wei Song, Wanqing Wei, Guipeng Hu, Jing Wu, Liming Liu, Xiulai Chen

Journal of Food Science and Biotechnology

[Objective ] This study aims to enhance the high osmotic pressure tolerance of Escherichia coli (E.coli ) for the efficient production of succinic acid.[Method ] Adaptive evolution,transcriptomic analysis,reverse metabolic engineering,and fermentation optimization were employed for improving E.coli tolerance to high osmotic pressure.[Result ] By analyzing the key factors influencing succinic acid production by fermentation,osmotic pressure was identified as the critical factor reducing succinic acid production efficiency.A mutant strain,E.coli FN-6,with tolerance to high osmotic pressure was obtained through adaptive evolution.The key gene cusC responsible for osmotic pressure tolerance was identified by transcriptomic analysis.After optimization of the expression level of cusC,the engineered …


Study On The Antioxidant Effects Of Natural Antioxidants And Their Derivatives On Anchovy (Engraulis Japonicus ) Oil, Xuyang Ding, Fujun Liu, Fawen Yin, Dayong Zhou Jun 2025

Study On The Antioxidant Effects Of Natural Antioxidants And Their Derivatives On Anchovy (Engraulis Japonicus ) Oil, Xuyang Ding, Fujun Liu, Fawen Yin, Dayong Zhou

Journal of Food Science and Biotechnology

[Objective ] The aim of this study is to evaluate and improve the antioxidant effects of natural antioxidants and their derivatives on anchovy (Engraulis japonicus ) oil.[Method ] The 60 ℃ accelerated oxidation model was used to evaluate the antioxidant effects of phytic acid,tea polyphenols (TP),rosemary extract,ascorbyl palmitate,vitamin E,tea polyphenol palmitate (TPP),and bamboo leaf extract on anchovy oil based on the peroxide value (POV ),thiobarbituric acid reactive substances (TBARS ) value,relative intensity of free radicals,and fatty acid composition.[Result ] TPP (single antioxidant ) and TPP+TP (composite antioxidant ) demonstrated the strongest antioxidant effects on anchovy oil.[Conclusion ] Although the antioxidant …


Rapid Determination Of Vitamin B 1 In Infant Formula Milk Powder By Fluorescent Microsphere Immunochromatographic Assay, Qianqian Lu, Ting Yang, Yongwei Feng, Jialin Hu, Hua Kuang, Chuanlai Xu, Lingling Guo Jun 2025

Rapid Determination Of Vitamin B 1 In Infant Formula Milk Powder By Fluorescent Microsphere Immunochromatographic Assay, Qianqian Lu, Ting Yang, Yongwei Feng, Jialin Hu, Hua Kuang, Chuanlai Xu, Lingling Guo

Journal of Food Science and Biotechnology

[Objective ] A fluorescent immunochromatographic assay was established based on vitamin B 1 monoclonal antibody to measure the vitamin B 1 in infant formula milk powder.[Method ] The performance of vitamin B 1 monoclonal antibody was evaluated.On this basis,a highly sensitive fluorescent immunochromatographic test strip was developed.[Result ] The vitamin B 1 monoclonal antibody showed high sensitivity,affinity,and specificity.The fluorescent immunochromatographic test strip showed a visual limit of detection (vLOD ) of 10 μg/kg,a cut-off value of 200 μg/kg,and a calculated limit of detection (cLOD ) of 2.056 μg/kg.In addition,the recovery rate of this method was 97.3%~115.4%.The test results of the …


Physicochemical Indicators , Microbial Diversity , And Volatile Substances In The Qupi And Quxin Of High-Temperature Daqu, Dan Xie, Cheng Wu, Wenyu Zhao, Jianfeng Hu, Pingyan Cheng, Yuanlin Bi, Junlin Yang, Xiaolong You, Diqiang Wang Jun 2025

Physicochemical Indicators , Microbial Diversity , And Volatile Substances In The Qupi And Quxin Of High-Temperature Daqu, Dan Xie, Cheng Wu, Wenyu Zhao, Jianfeng Hu, Pingyan Cheng, Yuanlin Bi, Junlin Yang, Xiaolong You, Diqiang Wang

Journal of Food Science and Biotechnology

[Objective ] This study aims to explore the physicochemical indicators,microbial diversity,and volatile substances in the Qupi (surface ) and Quxin (core) of high-temperature Daqu.[Method ] High-throughput sequencing and headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS ) were employed to compare the microbial diversity,volatile substances,and physicochemical indicators between Qupi and Quxin of three types of Daqu.[Result ] The moisture mass fraction in Qupi of the three types of Daqu was lower than that in Quxin (P<0.05),and the moisture mass fraction was the lowest in the Qupi of white Daqu (WP) and the highest in the Quxin of yellow Daqu (YX).The acidity of Qupi and Quxin were in the order of Quxin of black Daqu (BX) > YX > Qupi of black Daqu (BP) > Quxin of white Daqu (WX) > Qupi of yellow Daqu (YP) > WP.High-throughput sequencing results showed that Firmicutes and …


Assessing The Impact Of Spraying An E. Faecium Probiotic At Hatch And Supplementing Feed With A Triple-Strain Bacillus-Based Additive On Bco Lameness Incidence In Broiler Chickens, Khawla Alharbi, Anh Dang Trieu Do, Abdulaziz Alqahtani, Ruvindu Perera, Alexa Thomas, Antoine Meuter, Adnan Ali Khalaf Jun 2025

Assessing The Impact Of Spraying An E. Faecium Probiotic At Hatch And Supplementing Feed With A Triple-Strain Bacillus-Based Additive On Bco Lameness Incidence In Broiler Chickens, Khawla Alharbi, Anh Dang Trieu Do, Abdulaziz Alqahtani, Ruvindu Perera, Alexa Thomas, Antoine Meuter, Adnan Ali Khalaf

Poultry Science Faculty Publications and Presentations

Bacterial chondronecrosis with osteomyelitis (BCO) is a major cause of lameness in broiler chickens. This condition arises when bacteria from the gastrointestinal or aerosol tract migrate to infect bone microfractures, often exacerbated by rapid growth, reduced blood flow, and mechanical stress. As concerns about antibiotic resistance grow, probiotics have gained attention for their potential to improve gut health and reduce systemic bacterial load. This study evaluated the efficacy of a probiotic program comprising an Enterococcus faecium-based spray (2 x 10(9) CFU/bird at hatch) and a triple-strain Bacillus-based feed additive (B. subtilis 597, B. subtilis 600, and B. amyloliquefaciens 516 at …


Working Paper No. 98, Selma Mushkin And The Emergence Of The Field Of Health Economics, Sadie Kautz Jun 2025

Working Paper No. 98, Selma Mushkin And The Emergence Of The Field Of Health Economics, Sadie Kautz

Working Papers in Economics

This inquiry seeks to establish that through her effective efforts, Selma Mushkin promoted and shaped the field of Health Economics. Perhaps most importantly, she redefined health as a form of human capital that needed to be considered as both essential and integral to economic growth. In her 1958 paper: “Toward a Definition of Health Economics,” she defined the scope of this emerging field through emphasizing how improved resource allocation could lead to efficiency gains. Appearing as her 1962 article in the Journal of Political Economy, with “Health as an Investment” Mushkin advanced the idea that--much like education--public spending on health …


How Do Montessori-Based Activities Affect Behaviors For Older People With Dementia: A Literature Review, Isabel Quigley Jun 2025

How Do Montessori-Based Activities Affect Behaviors For Older People With Dementia: A Literature Review, Isabel Quigley

University Honors Theses

Objectives: This literature review is meant to analyze the development on the research on Montessori-based activities for adults with dementia.

Introduction: Baby Boomers are the largest generation to enter senior age (65+) in history due to the advancement in medicine to increase life expectancy. Dementia is a neurocognitive disease that affects parts of the brain related to memory and signs typically surface around 65 years old. Dementia is not considered healthy aging. Montessori-based activities have been recently developed as an intervention to encourage a feeling of accomplishment and success for adults with dementia.

Methods: Two electronic databases …