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Articles 96991 - 97020 of 934956
Full-Text Articles in Entire DC Network
Sacred Discrimination: Latvian Lutheran Clergy Against Ordination Of Women, Ģirts Rozners
Sacred Discrimination: Latvian Lutheran Clergy Against Ordination Of Women, Ģirts Rozners
Occasional Papers on Religion in Eastern Europe
This paper examines the resistance of contemporary Latvian Lutheran clergy to the ordination of women, rooted in historical factors and theological conservatism. It discusses the impact of Latvia's tumultuous history on theological discourse, revealing how practical concerns and traditionalism influenced the decision against women's ordination. Through clergy interviews, it uncovers a range of arguments used to justify this stance, exposing elements of sexism and neoconservatism. The clergy's prioritization of community identity over societal progress is evident, as they align with hierarchical leadership and resist external influences. This study highlights the disconnect between theological tradition and modern ethics, illustrating how theology …
"We Don’T Need No Ordination": Analysis Of The Discourse On Women’S Ordination In Latvian Online Media From 1999 To 2022, Emīls Zavelis, Sofija Anna Kozlova
"We Don’T Need No Ordination": Analysis Of The Discourse On Women’S Ordination In Latvian Online Media From 1999 To 2022, Emīls Zavelis, Sofija Anna Kozlova
Occasional Papers on Religion in Eastern Europe
This paper analyzes the discourse on women's ordination in the Evangelical Lutheran Church of Latvia (ELCL) as represented in Latvian online media from 1999 to 2022. The study combines qualitative and quantitative methodologies to examine the argumentative strategies employed by different discourse producers. The findings reveal significant differences in the use of gender equality concepts and other argumentative patterns between those in favor and those against women's ordination. These differences can be attributed to the distinct sociocultural contexts and epistemic grounds of the ELCL and the Evangelical Lutheran Church of Latvia Worldwide (ELCLW). The paper highlights the need for a …
The Missing Blue In Adventism: A Gap In Discipleship And Mission, Petr Cincala
The Missing Blue In Adventism: A Gap In Discipleship And Mission, Petr Cincala
Journal of Adventist Mission Studies
This article comes from the perspective of a researcher, a practical theologian, and a missiologist. The title The Missing Blue refers to the idea that light consists of three colors (Green, Red, Blue), and because God is light, the analogy of light is relevant for those who believe in the Triune God. We are called to worship the Creator God (represented by the green color) as Ruler of all the universe, God the consuming fire (Deut 4:24; Heb 12:29). Jesus (represented by the red color) teaches us to come to God as our friend, our daddy (John 15:15; Mark 14:36; …
Design And Optimization Of High-Efficiency Coreless Pcb Stator Axial Flux Pm Machines With Minimal Eddy And Circulating Current Losses, Yaser Chulaee, Greg Heins, Ali Mohammadi, Mark Thiele, Dan M. Ionel, Ben Robinson
Design And Optimization Of High-Efficiency Coreless Pcb Stator Axial Flux Pm Machines With Minimal Eddy And Circulating Current Losses, Yaser Chulaee, Greg Heins, Ali Mohammadi, Mark Thiele, Dan M. Ionel, Ben Robinson
Electrical and Computer Engineering Graduate Research
This paper proposes a systematic multi-step design procedure for highly efficient printed circuit board (PCB) stator coreless axial flux permanent magnet (AFPM) machines with minimal eddy and circulating current losses. The process begins with initial sizing, providing specific coefficients based on experience with multiple design projects. It continues with the optimization of the machine envelope design using an evolutionary algorithm and computationally efficient 3D finite element analysis (FEA) models. The subsequent step focuses on the detailed design of a PCB stator, aiming to minimize eddy and circulating current losses. Several open circuit loss mitigation techniques are proposed based on analytical …
Design Optimization Of A Direct-Drive Wind Generator With A Reluctance Rotor And A Flux Intensifying Stator Using Different Pm Types, Ali Mohammadi, Oluwaseun A. Badewa, Yaser Chulaee, Donovin D. Lewis, Somasundaram Essakiappan, Madhav Manjrekar
Design Optimization Of A Direct-Drive Wind Generator With A Reluctance Rotor And A Flux Intensifying Stator Using Different Pm Types, Ali Mohammadi, Oluwaseun A. Badewa, Yaser Chulaee, Donovin D. Lewis, Somasundaram Essakiappan, Madhav Manjrekar
Electrical and Computer Engineering Graduate Research
This paper presents a large-scale multi-objective design optimization for a direct-drive wind turbine generator concept that is based upon an experimentally validated computational model for a small-scale prototype motor of the same type. By integrating an outer reluctance-type rotor and a segmented stator with toroidally wound single-coil modules containing spoke-type PMs, the design optimization aims to minimize losses, active mass, and torque ripple while adhering to a power factor constraint. The AC windings and PMs are positioned in the stator and this concept enhances flux concentration, enabling the use of more affordable high energy non-rare-earth (special type) magnets. The exterior …
Large-Scale Design Optimization Of An Axial-Flux Vernier Machine With Dual Stator And Spoke Pm Rotor For Ev In-Wheel Traction, Ali Mohammadi, Yaser Chulaee, Aaron M. Cramer, Ion G. Boldea, Dan M. Ionel
Large-Scale Design Optimization Of An Axial-Flux Vernier Machine With Dual Stator And Spoke Pm Rotor For Ev In-Wheel Traction, Ali Mohammadi, Yaser Chulaee, Aaron M. Cramer, Ion G. Boldea, Dan M. Ionel
Electrical and Computer Engineering Graduate Research
This paper presents the optimization study targeting a specific drive cycle for a MAGNUS-type axial-flux permanent magnet vernier machine (AFPMVM). The proposed MAGNUS machine has a novel design with a dual-stator configuration, where only one stator is wound, with a high-polarity spoke permanent magnet (PM) rotor. The machine topology has a 3D flux path, which necessitates the analysis of a large finite element (FE) model. However, due to the computational complexity and time required for such a large FE model, a new approach was developed. This approach involves a computationally efficient finite element analysis (CE-FEA) model combined with a single …
Tonisha Haendiges '12-Christina "Mobile" Burrell '11, Tonisha Haendiges, Christina Burrell
Tonisha Haendiges '12-Christina "Mobile" Burrell '11, Tonisha Haendiges, Christina Burrell
Alumni Oral History Project
No abstract provided.
A Social History Of Opioids’ Crimedical Cycle, Jerel M. Ezell, Alex Rains, Babatunde Patrick Ajayi, Kyle Miller, Erin Augustine, Dawn Goddard-Eckrich, Elizabeth N. Kinnard, Michelle R. Lofwall
A Social History Of Opioids’ Crimedical Cycle, Jerel M. Ezell, Alex Rains, Babatunde Patrick Ajayi, Kyle Miller, Erin Augustine, Dawn Goddard-Eckrich, Elizabeth N. Kinnard, Michelle R. Lofwall
Behavioral Science Faculty Publications
Historically, public and political discourse around people who nonmedically use opioids has largely been rendered via a deficits-oriented lens, presenting this population as weak, disinterested, and deviant,and thus undesirable and ill-positioned for wellness and communal integration. These narratives, and subsequent policies used to engage people who use opioids, are intermittently formed and disrupted by geopolitical changes, as public and institutional attitudes, often driven by racial and class-related considerations, shift. As part of what we term the crimedical cycle, a population’s behaviors fluctuate between periods of medicalization and criminalization. Strengths-based theory, an offshoot of ‘positive psychology’ that helps contextualize the crimedical …
Rhetoric On Climate Change And The Effects On Public Perceptions, Hailey Mina Rose Dossey
Rhetoric On Climate Change And The Effects On Public Perceptions, Hailey Mina Rose Dossey
Undergraduate Scholarship
One of the extraordinary challenges humans are facing today is climate change and over the past two decades, the majority of the world has moved from questioning its legitimacy to accepting it as fact. With most people having accepted climate change as a reality, the goal of climate communication has shifted from spreading awareness to motivating audiences to take action. Through this analytical discussion, we will look into effective and ineffective methods of rhetoric as it can help guide us to a better understanding of why certain rhetoric that has previously been successful in other fields may not be the …
The Online Education Landscape, Digital Tools, And Covid-19, Rosetta S. Burk
The Online Education Landscape, Digital Tools, And Covid-19, Rosetta S. Burk
Undergraduate Scholarship
The 2020 COVID-19 pandemic has been the ultimate stress test for communication technologies, online learning methods, digital pedagogies, students and educators as the world faced school closures and emergency online learning situations during the pandemic lockdowns. There has been an influx in communication tools, digital paradigms, and teaching methodologies in addition to relying on existing digital tools and methods to continue students' education while unable to meet in person. The level of remote learning and digital movement is unprecedented and the results have been varied and illuminating. Research has been conducted on student attitudes in the immediate aftermath of the …
Pulling Back The Redwood Curtain: The Chinese Experience In Humboldt In The Nineteenth And Twentieth Centuries, Christopher J. Chu
Pulling Back The Redwood Curtain: The Chinese Experience In Humboldt In The Nineteenth And Twentieth Centuries, Christopher J. Chu
Undergraduate Scholarship
Chinese immigrants were integral to many industries of early California. In Humboldt, they worked in industries such as logging, fishing, and railroads, and provided essential services, such as farming, gathering, or laundry, to the local economy. Chinese immigrants in Humboldt County during the late 19th and early 20th centuries faced systemic discrimination that became a defining feature of the region's identity. State legislation, including the Chinese Exclusion Act of 1882, along with national economic instability, particularly the Long Depression (1873–1896) and xenophobic policies in California influenced legislation and attitudes in Humboldt County. These forces combined with long-held local prejudices and …
Artists’ Displacement And Rights: Citizenship, Care And Advocacy In The Networked Arts Sector In Europe, Mary Ann Devlieg
Artists’ Displacement And Rights: Citizenship, Care And Advocacy In The Networked Arts Sector In Europe, Mary Ann Devlieg
Doctoral
This thesis is situated at the intersection of arts, human rights and migration policies and practices. Contextualising five of my previous publications, the thesis is a critical reflection on my transectoral work as an advocate for the rights, recognition and professional development support of persecuted artists relocated to European Union countries for safety. Focusing on the decade 2009-2019, with a coda regarding significant events in 2023, the thesis exposes observed disconnects between a European cultural policy discourse stressing diversity and inclusion and the realities encountered by artists at risk seeking sanctuary. The thesis includes two chapters, an introduction and a …
Reclaiming Fantasy: The Female Protagonist In Works Of Visual Art, Literature And Film, Siobhán O'Connor
Reclaiming Fantasy: The Female Protagonist In Works Of Visual Art, Literature And Film, Siobhán O'Connor
Theses
‘Reclaiming Fantasy: The Female Protagonist in Works of Visual Art, Literature and Film’ charts representations of the female protagonist through the media in which the archetype is most prominent. From fairy tales to the slasher film, the female protagonist’s representation has been consistent with narratives that pertain to patriarchal constructions of gender. This is particularly true of genres that work on a level of ‘suspension of disbelief’, such as fantasy. This thesis presents findings on the reclamation of the female protagonist by female voices using a multidisciplinary approach, combining feminist theory, literary criticism, and visual art studies. From the 17th …
Differences In Student-Ai Interaction Process On A Drawing Task: Focusing On Students' Attitude Towards Ai And The Level Of Drawing Skills, Jinhee Kim, Yoonhee Ham, Sang-Soog Lee
Differences In Student-Ai Interaction Process On A Drawing Task: Focusing On Students' Attitude Towards Ai And The Level Of Drawing Skills, Jinhee Kim, Yoonhee Ham, Sang-Soog Lee
STEMPS Faculty Publications
Recent advances and applications of artificial intelligence (AI) have increased the opportunities for students to interact with AI in their learning tasks. Although various fields of scholarly research have investigated human-AI collaboration, the underlying processes of how students collaborate with AI in a student-AI teaming scenario have been scarcely investigated. To develop effective AI applications in education, it is necessary to understand differences in the student-AI interaction (SAI) process depending on students' characteristics. The present study attempts to fill this gap by exploring the differences in the SAI process amongst students with varying drawing proficiencies and attitudes towards AI in …
Education Out Loud Case Study: School For Life, Alexander Towne, Sladana Krstic, Jolanda Butler
Education Out Loud Case Study: School For Life, Alexander Towne, Sladana Krstic, Jolanda Butler
International Education Research
Children living in remote rural parts of Ghana experience inequality in basic education, in terms of both access and outcomes. This issue is particularly acute in the north of the country and for girls. For example, 30 percent of children in the north have no school nearby and 20 percent will never enroll. Furthermore, transparency and accountability within Ghana’s education system is weak. Generally, information is not disseminated in a way that is accessible to most citizens (for example it is not produced in a local language), which means they are denied the opportunity to understand and engage with the …
Education Out Loud Case Study: Iid, Alexander Towne, Sladana Krstic, Miriam Linder
Education Out Loud Case Study: Iid, Alexander Towne, Sladana Krstic, Miriam Linder
International Education Research
The education system in Bangladesh has undergone significant change since the country’s independence from Pakistan in 1971. In 1973 the community-sponsored school system, in which communities felt a moral obligation to take an active role in schools, was transformed into a centralised national system. This caused a shift in the sense of ownership of the education system and a disconnect between community and school. This in turn led to the development of a system, which lacks accountability and community monitoring, and has left the system vulnerable to shocks that disproportionately affect already disadvantaged students. Nevertheless, there have been some notable …
The Effects Of Affiliation, Proximity To Suicide, And Religiosity On Suicide Acceptance, Lindsey M. Gack
The Effects Of Affiliation, Proximity To Suicide, And Religiosity On Suicide Acceptance, Lindsey M. Gack
Master's Theses or Doctor of Nursing Practice
As suicide becomes increasingly more prominent in the lives of people, research is being conducted to investigate causes, prevention, and even opinions on the topic. The impact of religious affiliation and religiosity on people’s acceptance of suicide was investigated in the current study. This was examined by using The Religious Commitment Inventory-10 (RCI-10; Worthington et al., 2003), the Semantic Differential Scale Attitudes Towards Suicidal Behavior (SEDAS; Jenner & Niesing, 2000), and the Proximity to Suicide Scale (PSS; self-constructed). Results indicated that a higher religiosity score was correlated with a more understanding viewpoint of an attempted/committed suicide. Results also showed that …
Life Satisfaction In Motherhood: The Influence Of Mindfulness On Life Stressors, Shyann Schumacher, Shyann M. Schumacher
Life Satisfaction In Motherhood: The Influence Of Mindfulness On Life Stressors, Shyann Schumacher, Shyann M. Schumacher
Master's Theses or Doctor of Nursing Practice
Motherhood can be a stressful and highly demanding role. With the challenges a mother faces, many factors can influence satisfaction a mother experiences in their life. Within the many roles mothers play throughout their lives, mindfulness is found to benefit both women and their children and positively influence mothers’ relationships with their children or partner. This study investigated the role of mindfulness within life satisfaction among mothers’ experiences by examining levels of social support, mother’s age, work-life balance, and socioeconomic status as predictors of life satisfaction when moderated by mindfulness. Participants were sampled through social media including Facebook and Instagram. …
Exploring Psychological Factors Behind Consideration Of Cosmetic Surgery Through The Lens Of Social Comparison Theory, Noriene Verma
Exploring Psychological Factors Behind Consideration Of Cosmetic Surgery Through The Lens Of Social Comparison Theory, Noriene Verma
Master's Theses or Doctor of Nursing Practice
Cosmetic surgery refers to an extreme form of appearance modification whose sole purpose is to improve the appearance of the chosen area. Despite psychological concerns associated with repeated perform of cosmetic surgery, rates of cosmetic procedures are likely to increase in the coming years (American Society of Plastic Surgeons, 2020) and it warrants the need to study the potential motivators that encourage someone to opt for such procedures. Motivators of cosmetic surgery explored in this study are social media usage and self-esteem through the lens of Social Comparison Theory. Social comparison theory can explain the mechanisms behind one’s desire to …
A Portfolio In Creative Writing, Cooper Golden
A Portfolio In Creative Writing, Cooper Golden
Theses
When I began writing my thesis project, it was difficult to figure out which stories I had written during my time at UNA would fit. Before, during, and even between semesters, I wrote several stories that might have been good selections, but as they weren’t school assignments, I couldn’t include them. However, making my project has allowed me to go back to three stories and not only revise but improve on what I had written. Something else that I’ve noticed in my project is how diverse the genres of my stories have been. For example, in two stories I focus …
Multitudes, Madison Otter
Multitudes, Madison Otter
Master's Theses or Doctor of Nursing Practice
Identity is a fluid and layered experience enriched by contradictions, the power of interpersonal relationships, and continuous growth. Recognizing oneself as a blend of opposing traits—strong and tender, contemplative and restless—reveals that humanity is anything but black and white. With each passing day, we evolve, bringing new perspectives and insights with us. As Walt Whitman wrote, "[we] contain multitudes.''
Although no single artistic medium can fully capture this for me, using multiple modes of expression allows me to explore and approximate it. This realization was the origin of my thesis.
The process of creation itself, whether quick or deliberate, is …
A Comparative Analysis Of The American Revolution And The Jacobite Rebellions As “Civil Conflicts” Against Great Britain, Mandi Nash
Theses
British history consists of a large number of armed conflicts engaged against almost every country and continent in the world. While British history has a history rich with conflicts, two particular conflicts feature front and center when one is thinking in terms of a civil conflict– the American Revolution and the Jacobite Rebellions. A civil conflict is any conflict that takes place between citizens of the same country. Both the Jacobite Rebellions and the American Revolution were conflicts against Great Britain when Scotland and America were under British rule. Despite being two separate civil conflicts that differed in many ways, …
Higher Education Faculty Perceptions Of Chatgpt And The Influencing Factors: A Sentiment Analysis Of X, Yoseph Mamo, Helen Crompton, Diane Burke, Christine E. Nickel
Higher Education Faculty Perceptions Of Chatgpt And The Influencing Factors: A Sentiment Analysis Of X, Yoseph Mamo, Helen Crompton, Diane Burke, Christine E. Nickel
STEMPS Faculty Publications
ChatGPT, an AI chatbot developed by OpenAI, was released in November 2022, sparking a significant surge in global awareness and utilization of generative AI across various domains. Although recent studies have acknowledged the significance of ChatGPT in the education sector, they have yet to focus on exploring faculty attitudes toward ChatGPT. We gathered a comprehensive corpus of tweets containing “#ChatGPT” and “#highered” between November 30th, 2022, and April 30th, 2023. We analyzed data by triangulating VADER, NRC lexicon, and ground coding. Findings suggest that 40% of the expressed sentiments were positive, 51% were neutral, and 9% were negative. The study …
The Manifesto For Teaching And Learning In A Time Of Generative Ai: A Critical Collective Stance To Better Navigate The Future, Aras Bozkurt, Junhong Xiao, Robert Farrow, John Y. H. Bai, Chrissi Nerantzi, Stephanie Moore, Jon Dron, Christian M. Stracke, Lenandlar Singh, Helen Crompton, Apostolos Koutropoulos, Evgenii Terentev, Angelica Pazurek, Mark Nichols, Alexander M. Sidorkin, Eamon Costello, Steven Watson, Dónal Mulligan, Sarah Honeychurch, Charles B. Hodges, Mike Sharples, Andrew Swindell, Isak Frumin, Ahmed Tlili, Patricia J. Slagter Van Tryon, Melissa Bond, Maha Bali, Jing Leng, Kai Zhang, Mutlu Cukurnova, Thomas K. F. Chiu, Kyungmee Lee, Stefan Hrastinski, Manuel B. Garcia, Ramesh Chander Sharma, Bryan Alexander, Olaf Zawacki-Richter, Henk Huijser, Petar Jandrić, Chanjin Zheng, Peter Shea, Josep M. Duart, Chryssa Themeli, Anton Vorochkov, Sunagül Sani-Bozkurt, Robert L. Moore, Tutaleni Iita Asino
The Manifesto For Teaching And Learning In A Time Of Generative Ai: A Critical Collective Stance To Better Navigate The Future, Aras Bozkurt, Junhong Xiao, Robert Farrow, John Y. H. Bai, Chrissi Nerantzi, Stephanie Moore, Jon Dron, Christian M. Stracke, Lenandlar Singh, Helen Crompton, Apostolos Koutropoulos, Evgenii Terentev, Angelica Pazurek, Mark Nichols, Alexander M. Sidorkin, Eamon Costello, Steven Watson, Dónal Mulligan, Sarah Honeychurch, Charles B. Hodges, Mike Sharples, Andrew Swindell, Isak Frumin, Ahmed Tlili, Patricia J. Slagter Van Tryon, Melissa Bond, Maha Bali, Jing Leng, Kai Zhang, Mutlu Cukurnova, Thomas K. F. Chiu, Kyungmee Lee, Stefan Hrastinski, Manuel B. Garcia, Ramesh Chander Sharma, Bryan Alexander, Olaf Zawacki-Richter, Henk Huijser, Petar Jandrić, Chanjin Zheng, Peter Shea, Josep M. Duart, Chryssa Themeli, Anton Vorochkov, Sunagül Sani-Bozkurt, Robert L. Moore, Tutaleni Iita Asino
STEMPS Faculty Publications
This manifesto critically examines the unfolding integration of Generative AI (GenAI), chatbots, and algorithms into higher education, using a collective and thoughtful approach to navigate the future of teaching and learning. GenAI, while celebrated for its potential to personalize learning, enhance efficiency, and expand educational accessibility, is far from a neutral tool. Algorithms now shape human interaction, communication, and content creation, raising profound questions about human agency and biases and values embedded in their designs. As GenAI continues to evolve, we face critical challenges in maintaining human oversight, safeguarding equity, and facilitating meaningful, authentic learning experiences. This manifesto emphasizes that …
Underuse Of Pragmatic Markers Among Non-Native English Speakers: Causes And Suggestions For Interventions, Arwa Abdullah S. Alqahtani
Underuse Of Pragmatic Markers Among Non-Native English Speakers: Causes And Suggestions For Interventions, Arwa Abdullah S. Alqahtani
Browse all Theses and Dissertations
Pragmatic markers (PMs)—those optional markers used in conversations to facilitate communication—have been gaining attention among researchers in the field of pragmatics in the past three decades or so. Proper use of PMs contributes to the success of any interaction in real-life conversations. However, they are often underused by non-native English speakers (NNSs). This paper critically reviews the existing research literature to investigate the causes of the underuse of PMs among NNSs, focusing on linguistic and extralinguistic factors that might contribute to the limited use of PMs. The findings of this study show that the distinctive nature of pragmatic development in …
Retracing Their Tracks: The Rediscovery And Memorialization Efforts Of The National Orphan Train Project, Joanna Lockwood
Retracing Their Tracks: The Rediscovery And Memorialization Efforts Of The National Orphan Train Project, Joanna Lockwood
Master's Theses or Doctor of Nursing Practice
Between 1854 and 1929, the placement system that became known as the orphan trains in modern scholarship, removed neglected and orphaned children from the dangerous streets of New York City and attempted to place them into homes across America that would benefit their futures. While this charity-run program was well-intentioned, the estimated two hundred fifty thousand children of the orphan trains experienced a mix of encounters upon their placements. Although many homes provided a caring environment for these children, others did not. After years of struggle during their early childhood, numerous children had not only lost their parents and were …
Bacterial Metallophores As Potential Agents In Heavy Metal Bioremediation, Mohammed Ahmed
Bacterial Metallophores As Potential Agents In Heavy Metal Bioremediation, Mohammed Ahmed
Electronic Theses and Dissertations
Heavy metal pollution is a significant threat to human health and the environment. Bacteria have evolved diverse mechanisms to adapt to these pollutants, including the secretion of small molecules called metallophores. Bacterial metallophores are metabolites excreted in response to toxic metal stress to protect the bacterial cell. These small molecules have shown versatility in potential applications for the remediation of heavy metals. This research focuses on constructing a library of bacterial metallophores, which will facilitate the assessment of their metal-binding properties when exposed to different metals. The study has two main areas of investigation: Firstly, it involves isolating and characterizing …
Language Variation: Arabic Dialects In Madinah, Saudi Arabia, Arwa Alsindy
Language Variation: Arabic Dialects In Madinah, Saudi Arabia, Arwa Alsindy
Electronic Theses and Dissertations
Arabic sociolinguistics, namely Arabic dialectology, is a demanding area of study. Therefore, the current research was conducted to fulfill some of those needs. Qualitative methods were applied in order to answer the research questions: What are the salient linguistic features of the Hijazi Hadari dialect, Urban Bedouins Hijazi dialect, and the emerging Saudi Arabic White dialect in Madinah? When are these dialects used? What changes or accommodations are made to these dialects when communicating with the other social groups? To provide answers to those questions, observations and interviews were applied. By applying the SPEAKING model (Hymes, 1974), six participants (three …
Astrocytic Mitochondrial Transfer To Brain Endothelial Cells And Pericytes In Vivo Increases With Aging, Gopal V. Velmurugan, Hemendra J. Vekaria, Samir P. Patel, Patrick G. Sullivan, W. Brad Hubbard
Astrocytic Mitochondrial Transfer To Brain Endothelial Cells And Pericytes In Vivo Increases With Aging, Gopal V. Velmurugan, Hemendra J. Vekaria, Samir P. Patel, Patrick G. Sullivan, W. Brad Hubbard
Neurology Faculty Publications
Intercellular mitochondrial transfer (IMT) is an intriguing biological phenomenon where mitochondria are transferred between different cells and notably, cell types. IMT is physiological, occurring in normal conditions, but also is utilized to deliver healthy mitochondria to cells in distress. Transferred mitochondria can be integrated to improve cellular metabolism, and mitochondrial function. Research on the mitochondrial transfer axis between astrocytes and brain capillaries in vivo is limited by the cellular heterogeneity of the neurovascular unit. To this end, we developed an inducible mouse model that expresses mitochondrial Dendra2 only in astrocytes and then isolated brain capillaries to remove all intact astrocytes. …
Using Digital Pathology To Analyze The Murine Cerebrovasculature, Dana M. Niedowicz, Jenna L. Gollihue, Erica M. Weekman, Panhavuth Phe, Donna M. Wilcock, Christopher M. Norris, Peter T. Nelson
Using Digital Pathology To Analyze The Murine Cerebrovasculature, Dana M. Niedowicz, Jenna L. Gollihue, Erica M. Weekman, Panhavuth Phe, Donna M. Wilcock, Christopher M. Norris, Peter T. Nelson
Neurology Faculty Publications
Research on the cerebrovasculature may provide insights into brain health and disease. Immunohistochemical staining is one way to visualize blood vessels, and digital pathology has the potential to revolutionize the measurement of blood vessel parameters. These tools provide opportunities for translational mouse model research. However, mouse brain tissue presents a formidable set of technical challenges, including potentially high background staining and crossreactivity of endogenous IgG. Formalin-fixed paraffin-embedded (FFPE) and fixed frozen sections, both of which are widely used, may require different methods. In this study, we optimized blood vessel staining in mouse brain tissue, testing both FFPE and frozen fixed …