Digital Commons Network^™

Invl, An Invasin-Like Adhesin, Is A Type Ii Secretion System Substrate Required For Acinetobacter Baumannii Uropathogenesis, Clay D Jackson-Litteken, Gisela Di Venanzio, Nguyen-Hung Le, Nichollas E Scott, Bardya Djahanschiri, Jesus S Distel, Evan J Pardue, Ingo Ebersberger, Mario F Feldman

Open Access Publications

Acinetobacter baumannii is an opportunistic pathogen of growing concern, as isolates are commonly multidrug resistant. While A. baumannii is most frequently associated with pulmonary infections, a significant proportion of clinical isolates come from urinary sources, highlighting its uropathogenic potential. The type II secretion system (T2SS) of commonly used model Acinetobacter strains is important for virulence in various animal models, but the potential role of the T2SS in urinary tract infection (UTI) remains unknown. Here, we used a catheter-associated UTI (CAUTI) model to demonstrate that a modern urinary isolate, UPAB1, requires the T2SS for full virulence. A proteomic screen to identify …

Ultrasmall Nanodiamonds: Perspectives And Questions, Shery L.Y. Chang, Philipp Reineck, Anke Krueger, Vadym Mochalin

Chemistry Faculty Research & Creative Works

Nanodiamonds are at the heart of a plethora of emerging applications in areas ranging from nanocomposites and tribology to nanomedicine and quantum sensing. The development of alternative synthesis methods, a better understanding, and the availability of ultrasmall nanodiamonds of less than 3 nm size with a precisely engineered composition, including the particle surface and atomic defects in the diamond crystal lattice, would mark a leap forward for many existing and future applications. Yet today, we are unable to accurately control nanodiamond composition at the atomic scale, nor can we reliably create and isolate particles in this size range. In this …

Impacts Of Population Level Environmental Contaminants, Sex Hormones, And Fibroblast Cell Subsets On Systemic Sclerosis (Ssc), Noelle N. Kosarek

Dartmouth College Ph.D Dissertations

Systemic sclerosis (SSc) is a rare autoimmune disease characterized by fibrosis of the skin and internal organs, vascular abnormalities, and autoantibody formation. The etiology of SSc is unknown, though the disease is thought to arise in genetically predisposed individuals after exposure to an environmental factor. There are few FDA approved disease modifying medications available to treat SSc.

The express aims of this dissertation are tripart. First, we aimed to validate a 3D tissue model known as the self-assembled skin equivalent (saSE) model. Second, we sought to describe the geographic distribution of SSc in a US Medicare population to better understand …

Technology Use By Registered Dietitians For Patient Care In An Outpatient Setting, Donika Emerllahu

Theses

Background Technology use in digital health tools has increased significantly since the COVID-19 pandemic disrupted many in-office patient care services. Although healthcare practices have adopted several technologies, they remain surrounded by a saturated increase in consumer-based wearables and smartphone applications. As a result, attitudes and acceptability toward integrating these technologies for patient care are evolving. Digital health tools is an umbrella term including several technologies utilized among health care providers for the purpose of patient care. The inclusion of digital health tools by Registered Dietitian Nutritionists’ (RDNs) in practice provides a more robust and personalized approach to patients and their …

Mycn-Driven Fatty Acid Uptake Is A Metabolic Vulnerability In Neuroblastoma, Ling Tao, Mahmoud A Mohammad, Giorgio Milazzo, Myrthala Moreno-Smith, Tajhal D Patel, Barry Zorman, Andrew Badachhape, Blanca E Hernandez, Amber B Wolf, Zihua Zeng, Jennifer H Foster, Sara Aloisi, Pavel Sumazin, Youli Zu, John Hicks, Ketan B Ghaghada, Nagireddy Putluri, Giovanni Perini, Cristian Coarfa, Eveline Barbieri

Faculty, Staff and Students Publications

Neuroblastoma (NB) is a childhood cancer arising from sympatho-adrenal neural crest cells. MYCN amplification is found in half of high-risk NB patients; however, no available therapies directly target MYCN. Using multi-dimensional metabolic profiling in MYCN expression systems and primary patient tumors, we comprehensively characterized the metabolic landscape driven by MYCN in NB. MYCN amplification leads to glycerolipid accumulation by promoting fatty acid (FA) uptake and biosynthesis. We found that cells expressing amplified MYCN depend highly on FA uptake for survival. Mechanistically, MYCN directly upregulates FA transport protein 2 (FATP2), encoded by SLC27A2. Genetic depletion of SLC27A2 impairs NB survival, and …

Expanding Anti-Cd38 Immunotherapy For Lymphoid Malignancies, Xu Wang, Xinfang Yu, Wei Li, Praveen Neeli, Ming Liu, Ling Li, Mingzhi Zhang, Xiaosheng Fang, Ken H Young, Yong Li

Faculty, Staff and Students Publications

BACKGROUND: Lymphoid neoplasms, including multiple myeloma (MM), non-Hodgkin lymphoma (NHL), and NK/T cell neoplasms, are a major cause of blood cancer morbidity and mortality. CD38 (cyclic ADP ribose hydrolase) is a transmembrane glycoprotein expressed on the surface of plasma cells and MM cells. The high expression of CD38 across MM and other lymphoid malignancies and its restricted expression in normal tissues make CD38 an attractive target for immunotherapy. CD38-targeting antibodies, like daratumumab, have been approved for the treatment of MM and tested against lymphoma and leukemia in multiple clinical trials.

METHODS: We generated chimeric antigen receptor (CAR) T cells targeting …

Human Metapneumovirus Phosphoprotein Independently Drives Phase Separation And Recruits Nucleoprotein To Liquid-Like Bodies, Kerri Beth Boggs, Kearstin Edmonds, Nicolas Cifuentes-Munoz, Farah El Najjar, Conny Ossandón, Mckenna Roe, Chao Wu, Carole L Moncman, Trevor P Creamer, Gaya K Amarasinghe, Daisy W Leung, Rebecca Ellis Dutch

Open Access Publications

Human metapneumovirus (HMPV) inclusion bodies (IBs) are dynamic structures required for efficient viral replication and transcription. The minimum components needed to form IB-like structures in cells are the nucleoprotein (N) and the tetrameric phosphoprotein (P). HMPV P binds to the following two versions of the N protein in infected cells: N-terminal P residues interact with monomeric N (N

Multiphysics-Based Computational Modeling Of Ocular Therapeutics, Jabia Mostofa Chowdhury

Electronic Theses and Dissertations Archive

Detecting ocular conditions and initiating treatment at the earliest is essential for preserving the remaining visual health in various ocular diseases such as glaucoma, diabetic retinopathy, and inner retinal diseases such as retinoblastoma. Ocular treatment strategies include topical administration of drugs (e.g., Timolol for glaucoma), ocular surgeries (e.g., trabeculoplasty), gene therapy (e.g., for treating age related macular degeneration), light-based (e.g., laser) therapies and direct injection (e.g., intravitreal injections) of drugs depending on the target site of the ocular therapy. Topical administration of drugs to the anterior segment and photothermal therapy of the posterior segment of the eye are among the …

Robust Explainability: A Tutorial On Gradient-Based Attribution Methods For Deep Neural Networks, Ian E. Nielsen, Dimah Dera, Ghulam Rasool, Nidhal Bouaynaya, Ravi P. Ramachandran

Electrical and Computer Engineering Faculty Publications

With the rise of deep neural networks, the challenge of explaining the predictions of these networks has become increasingly recognized. While many methods for explaining the decisions of deep neural networks exist, there is currently no consensus on how to evaluate them. On the other hand, robustness is a popular topic for deep learning research; however, it is hardly talked about in explainability until very recently. In this tutorial paper, we start by presenting gradient-based interpretability methods. These techniques use gradient signals to assign the burden of the decision on the input features. Later, we discuss how gradient-based methods can …

Gata Now!, Georgia Southern University

Athletics: News & Publications

• Class of 2022
• Season Tickets
• Welcome Back to Statesboro
• ERK Russell Found Donor Weekend
• 2022 Football Schedule
• Full Schedule
• Headlines

Gata Now!, Georgia Southern University

Athletics: News & Publications

• Class of 2022
• Season Tickets
• Welcome Back to Statesboro
• ERK Russell Found Donor Weekend
• 2022 Football Schedule
• Full Schedule
• Headlines

Gain-Of-Signal Assays For Probing Inhibition Of Sars-Cov-2 Mpro/3clpro In Living Cells, Seyed Arad Moghadasi, Morgan A Esler, Yuka Otsuka, Jordan T Becker, Sofia N Moraes, Constance B Anderson, Srinivas Chamakuri, Christopher Belica, Chloe Wick, Daniel A Harki, Damian W Young, Louis Scampavia, Timothy P Spicer, Ke Shi, Hideki Aihara, William L Brown, Reuben S Harris

Faculty, Staff and Students Publications

The main protease, Mpro, of SARS-CoV-2 is required to cleave the viral polyprotein into precise functional units for virus replication and pathogenesis. Here, we report quantitative reporters for Mpro function in living cells in which protease inhibition by genetic or chemical methods results in robust signal readouts by fluorescence (enhanced green fluorescent protein [eGFP]) or bioluminescence (firefly luciferase). These gain-of-signal systems are scalable to high-throughput platforms for quantitative discrimination between Mpro mutants and/or inhibitor potencies as evidenced by validation of several reported inhibitors. Additional utility is shown by single Mpro amino acid variants and structural information combining to demonstrate that …

Investigating The Role Of Amyloid Beta Peptides In Inflammation And Alzheimer’S Disease Pathogenesis, Nguyen Le

Theses and Dissertations

Alzheimer’s Disease (AD) is a neurodegenerative disease characterized by severe memory decline and cognitive impairments. In AD patients’ brains, aggregates of amyloid beta (Aβ) peptides, called amyloid plaques, are hypothesized to trigger innate immune responses that contribute to AD pathogenesis. Interestingly, recent studies demonstrated that an increased level of Aβ protected the host against pathogen infections. Using Drosophila as a model organism, our preliminary data showed that the loss of the Drosophila APP homolog, APPL, led to immune deficits against parasite infection, and that overexpression of Drosophila Aβ produced an inflammatory phenotype. These findings suggested that Aβ might be required …

Tannic Acid Exhibits Antiangiogenesis Activity In Nonsmall-Cell Lung Cancer Cells, Elham Hatami, Prashanth K.B. Nagesh, Mohammed Sikander, Anupam Dhasmana, Subhash C. Chauhan, Meena Jaggi, Murali M. Yallapu

School of Medicine Publications

Nonsmall-cell lung cancer (NSCLC) is the most common type of lung cancer, with a dismal prognosis. NSCLC is a highly vascularized tumor, and chemotherapy is often hampered by the development of angiogenesis. Therefore, suppression of angiogenesis is considered a potential treatment approach. Tannic acid (TA), a natural polyphenol, has been demonstrated to have anticancer properties in a variety of cancers; however, its angiogenic properties have yet to be studied. Hence, in the current study, we investigated the antiproliferative and antiangiogenic effects of TA on NSCLC cells. The (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) assay revealed that TA induced a dose- and time-dependent …

Aberrant Dna Hydroxymethylation Reshapes Transcription Factor Binding In Myeloid Neoplasms, Jia Li, Tingting Hong, Yue Wei, Lei Guo, Minjung Lee, Hui Yang, Caleb Class, Yaling Yang, Xiaoqiong Wang, Hua He, Stefan Siwko, M James You, Yubin Zhou, Guillermo Garcia-Manero, Yun Huang

Faculty, Staff and Student Publications

Epigenetic abnormalities in DNA hydroxymethylation (5hmC) have been detected in patients with myeloid neoplasms, suggesting that 5hmC might act as a valuable epigenetic mark to reflect the disease status of myeloid neoplasms. Here, we report systematic genome-wide mapping of the DNA hydroxymethylomes in over 70 patients with myeloid neoplasms. Our integrative analysis leads to the identification of distinct 5hmC signatures that can sensitively discriminate patients from healthy individuals. At the molecular level, we unveiled dynamic 5hmC changes within key transcription factor (e.g., the CEBP family) binding motifs that are essential for hematopoiesis and myeloid lineage specification. 5hmC redistribution was found …

The Reality Of Using Social Networking Sites Among Students Enrolled In The Social Responsibility Course In Light Of Some Variables واقع استخدام مواقع التواصل الاجتماعي لدى طلبة مساق المسؤولية المجتمعية في ضوء بعض المتغيرات, Dr. Mohammed Kh. Muflih, Heba M. Omari

Jordanian Educational Journal

Abstract: This study aimed at investigating the reality of using social networking sites among university students who were registered in the course of social responsibility at the Jordan University Science and technology. The researchers developed a questionnaire and confirmed its validity and reliability before initiating data collection process. A purposive sampling method was used to recruit 400 students of the course were chosen purposely at the beginning of the first semester 2018-2019. The results of the study showed that YouTube was the most preferred site used by the study sample and they primarily used the social networking sites for communicating …

Role Of Faculty Members Of Yarmouk University In Developing Students’ Citizenship And Its Relationship To The Level Of Students Morale As Perceived By Themselves دور أعضاء هيئة التدريس في جامعة اليرموك في تنمية المواطنة لدى الطلبة وعلاقتها بمستوى الروح المعنوية لديهم من وجهة نظر الطلبة أنفسهم, Dima A. Al-Refaee, Prof. Saleh N. Olimat

Jordanian Educational Journal

Abstract: The present study aimed to identify the role of the faculty members in developing students’ citizenship and the level of students morale at Yarmouk University from the viewpoints of the students themselves, also to examine the correlation between the role of faculty members in the development of citizenship and the level of morale among students at Yarmouk University. The sample of the study was selected by a stratified random sampling of 5% of the study population from Yarmouk university students. The number of respondents was 1487 students. The results showed that the level of citizenship among students from the …

A Critical Look At The Importance Of Teaching National Identity In Algerian Efl Classrooms: Perceptions Of Public Middle And Secondary School Teachers نظرة فاحصة على أهمية تدريس الهوية الوطنية في صفوف اللغة الإنجليزية كلغة أجنبية في الجزائر: تصورات معلمي المدارس المتوسطة والثانوية الحكومية, Miral Bassaid, Prof. Oqlah M. Smadi

Jordanian Educational Journal

Abstract: Over the past decades, studies investigating the concept of national identity within educational settings have grown increasingly prevalent and in demand. Ergo, the following study has set out to examine the perceptions of EFL teachers regarding the importance of incorporating national identity within newly developed English textbooks used in public middle and secondary schools in Algeria. The researchers elicited the views of 112 Algerian teachers during the second semester of the academic year 2017-2018 regarding the efficiency of home-grown textbooks in teaching national and local matters as well as their role in helping Algerian students foster their intercultural competence. …

The Role Of The Sports Media To Reducing Psychological Anxiety Among Players Of Premium Class Volleyball Clubs In Jordan From Their Point Of View دور الإعلام الرياضي في الحد من القلق النفسي لدى لاعبي أندية الدرجة الممتازة للكرة الطائرة في الأردن من وجهة نظرهم, Dr. Ahmad Khalid Al- Thnaibat, Hamad Sattar Al- Ea'nezy

Jordanian Educational Journal

Abstract: The study aimed at investigating the role of sports media to reducing psychological anxiety among players of premium class volleyball clubs in Jordan from their point of view, The study sample comprised of (71) players, who were intentionally selected from the population of the study. The descriptive methodology was used. A questionnaire was developed to measure the role of sports media in reducing psychological anxiety among players of premium class volleyball clubs from their point of view. It consisted of four dimensions (rewards, coach, social and abilities dimensions). The results showed that the level of sports media to reducing …

A Comparative Study Of The Executive Functions Of Students With Learning Disabilities And Normal Students دراسة مقارنة للوظائف التنفيذية لكل من الطلبة ذوي صعوبات التعلم والطّلبة العاديين, Rawan Adnan Ibdah, Prof. Jamil Mahmoud Al-Smadi

Jordanian Educational Journal

Abstract: This study aimed to identify the differences in the executive functions of students with learning disabilities, and ordinary students. It is a comparative descriptive study, during which the researchers applied a diagnostic test in the form of a scale called BRIEF2. It is a test in the form of a battery to assess the behavior of students.It includes forms to be filled by the class room teacher and the special education teacher.The scale consists of 59 items distributed over (9) dimensions for the estimation of teachers and to ensure the characteristics and validity of the scale of executive functions.The …

The Effect Of Using Oral And Digital Storytelling Strategies In Improving Critical Listening Skills Among The Female Students Of The Basic Ninth Grade In Jordan أثر استخدام استراتيجيتي السرد القصصي الشفوي والرقمي في تحسين مهارات الاستماع الناقد لدى طالبات الصف التاسع الأساسي في الأردن, Dr. Lamia’ Muhammad Omoush, Dr. Feras Mahmoud Alslaiti

Jordanian Educational Journal

Abstract: The aim of the study was to investigate the effect of using oral and digital storytelling strategies in improving critical listening skills among female students of the basic ninth grade in Jordan. The participants of the study consisted of (86) female students of the ninth grade, who were selected from the schools of the brigade of Bani Obaid - Irbid. The students were set in three groups. The oral storytelling strategy was used in teaching the first experimental group. The digital storytelling strategy was used in teaching of the second experimental group, while the usual method was used to …

The Effect Of Individualized Education By Using Multimedia On The Academic Achievement In Computer Course Of The First Secondary Grade Female Students In Jordan أثر تفريد التعليم باستخدام الوسائط المتعددة في التحصيل الدراسي في مادة الحاسوب لدى طالبات الصف الأول الثانوي في الأردن, Areej Emlawi Al-Khawaldah, Prof. Muhannad Anwar Al-Shboul

Jordanian Educational Journal

Abstract: This study aimd at investigating the effect of individualized education by using multimedia on the academic achievement in computer course of the first secondary grade female students in Jordan. The study sample consisted of (60) female students, from first secondary grade students, selected from a public school in MafraqGasapa directorate of education. The study sample assigned randomly into two groups: experimental group of (30) female students, which studied through individualized education by using multimedia, and the control group of (30) female students, who studied through using conventional approach. To achieve the objectives of the study, the researchers used Quasi-experimental …

Semantically Mapping Information About A User Onto A Digital Image Uploaded By A User, Ramprasad Sedouram

Defensive Publications Series

The present disclosure describes computer-implemented systems and methods for semantically tagging digital images (e.g., profile photos) uploaded by a user based on information about the user. A viewing user may interact with the semantically tagged digital images to learn more about the user that uploaded the digital image without having to navigate away from the digital image.

Using Existing Vehicle Proximity Sensors For Remote Mapping Of Empty Parking Spaces, D. Shin

Defensive Publications Series

A vehicle (e.g., automobile, motorcycle, a bus, a recreational vehicle (RV), a semi-trailer truck, a tractor or other type of farm equipment, a train, a plane, a helicopter, etc.) often has proximity sensors to detect adjacent objects. A head unit of the vehicle may use sensor data such as proximity sensor data to detect an empty parking space. The head unit may then report the detected empty parking space to a cloud computing system, such as a mapping service. Data from multiple vehicles may be used to crowd source a relatively detailed understanding of the amount of empty parking spaces …

Enabling Touch Capabilities On Auto Dashboard Screens Without Touchscreens, D. Shin

Defensive Publications Series

A vehicle (e.g., automobile, motorcycle, a bus, a recreational vehicle (RV), a semi-trailer truck, a tractor or other type of farm equipment, a train, a plane, a helicopter, etc.) may have displays to make visual presentations of useful information such as maps, personalized routes, media, etc. Often a separate computing device (e.g., a smartphone, a laptop computer, a tablet computer, smartglasses, a portable gaming system, a laptop computer, a smartwatch, etc.) may provide the visual presentation data for the vehicle. Newer model vehicles typically have touchscreen displays, but some vehicles may have displays without an associated touchscreen. In those cases, …

Podium Presentation: Impact Of A Research Basics Course On Hospital-Based Nursing Research Confidence Levels: A Program Evaluation, Trisha Saul, Teresa Bigand

2022 Providence Nurse Research Conference

Background:

The American Nurse Credentialing Center’s (ANCC) Magnet® recognition promotes best patient outcomes and nurse practice environments within a healthcare organization and requires nurses to conduct research and disseminate findings. However, while undergraduate nursing programs recognize the need to provide research knowledge, current pre-licensure curriculum is not designed to engage students in research activities to foster competency in this skill. Despite research competencies endorsed by select national nursing organizations and the Magnet® designation requirements, RN competency levels for participating in research activities are reported to be suboptimal. Thus, best practice recommends a formal program to train and guide nurses in …

Researching European Community Competition Law: A Practical Guide And Selective Bibliography, Amanda Lynn Parks

Florida Journal of International Law

No abstract provided.

A Delicate Balance: The Effectiveness Of Apartheid Reforms In The Struggle For The Future Of South Africa, Kimberlee Ann Scalia

Florida Journal of International Law

This article will first discuss the history of Apartheid in South Africa leading up to the reforms instituted by de Klerk. Then, the effectiveness of these reforms will be analyzed in the South African and international contexts and, more particularly, in the wake of the repeal of one of the pillars of apartheid. Finally, this article will consider how truly effective reform may be achieved.

Private Lives And Public Eyes: Privacy In The United States And Japan, Dan Rosen

Florida Journal of International Law

No abstract provided.

The United States Of America: Champion Of The Rule Of Law Or The New World Order?, Jeffrey F. Addicott

Florida Journal of International Law

No abstract provided.