Digital Commons Network^™

Characterizing Modulators Of Protease-Activated Receptors With A Calcium Mobilization Assay Using A Plate Reader, Jacob T. Derousse, Chris Dockendorff

Chemistry Faculty Research and Publications

Changes in calcium concentration in cells are rapidly monitored in a high-throughput fashion with the use of intracellular, fluorescent, calcium-binding dyes and imaging instruments that can measure fluorescent emissions from up to 1,536 wells simultaneously. However, these instruments are much more expensive and can be challenging to maintain relative to widely available plate readers that scan wells individually. Described here is an optimized plate reader assay for use with an endothelial cell line (EA.hy926) to measure the protease-activated receptor (PAR)-driven activation of G_αq signaling and subsequent calcium mobilization using the calcium-binding dye Fluo-4. This assay has been used to …

A Revised Synthesis Of The Cationic Lipids Dmri And Mlri, Benjamin A. Kostic

College of Arts & Sciences Senior Theses

Lipid nanoparticles (LNPs) are an increasingly utilized method of intracellular delivery for nucleic acids. The COVID-19 vaccines from Moderna and Pfizer-BioNTech have used LNP technology to combat the recent global SARS-CoV-2 pandemic, saving millions of lives.^1,2 These vaccines consist of a lipid formulation that binds to the anionic phosphate groups of mRNAs and serves as an effective way to transport the nucleic acid across membranes into cells.⁹The mRNA encodes a viral protein. When introduced to cells, the viral protein is produced so that the immune system then can generate an appropriate antibody response.³ However, this approach …

Mathematics Majors In Medical School Admissions: A Comparative Evaluation Of Mcat And Gpa Performance, Morgan Baker

Theses/Capstones/Creative Projects

Choosing a major as an incoming undergraduate student can be very stressful. This study investigates the differences in success that come with choice of undergraduate major, particularly focusing on the performance of mathematics majors. A large majority of medical school applicants come from a biological sciences background. Despite this preference, there is evidence that students from nontraditional majors produce higher Medical College Admission Test (MCAT) scores and superior grade point averages (GPAs). Utilizing data visualization and analysis through R programming, this research examines public data from the Association of American Medical Colleges (AAMC) to understand the benefits of pursuing a …

Cofinal Types And Bounding Numbers: A Literature Review, Spencer Chapman

Math Honors Theses

No abstract provided.

Benefits Of Speech Language Pathologists On Elementary School Students, Gisel Reynoso

Capstone Projects and Master's Theses

This senior capstone research project outlines a research endeavor aimed at exploring the multifaceted impact of speech-language pathologists (SLPs) on elementary school students, with a particular focus on developing educators, enhancing diversity, and fostering subject matter expertise. Motivated by firsthand experiences in service-learning and recognizing the significant challenges students face due to communication disorders, this study seeks to investigate how SLPs can effectively support students while contributing to educators' professional development and promoting inclusivity. Through a mixed-methods approach, including interviews, surveys, and data analysis, this senior capstone research project aims to provide valuable insights into the transformative role of SLPs …

How To Explain Allen-Manandhar’S Method To Beginner Mathematicians : A Convergence Analysis Of A Hybrid Method For Variable-Coefficient Boundary Value Problems, Rebecca Scariano

Honors Theses

In this project, analogies are employed to make complex math concepts approachable to beginners who may only have a basic understanding of calculus and linear algebra. Serving as the focal point of this project, Allen-Manandhar’s method solves an equation, known as an ordinary differential equation (ODE). The mentioned equation with its coefficients is comparable to a pie recipe with ingredients. With the outcome to a recipe seen as its solution, the solution to our pie recipe is a perfectly baked pie, as in without error. The chosen method for baking a pie then classifies as its baking approach that when …

Innovative Rate Design As A Free Market Solution To Climate, Resiliency, And Economic Challenges, Brendan J. Moore

LSU New Orleans Theses and Dissertations

A century-old problem of electricity rate design is cost-shifting between ratepayers (Wellinghoff, J. & Tong, J., 2015). A much newer cost-shifting example of great and increasing importance happens whenever ratepayer generated “renewable energy” is sold to the grid—all too often, this is accused of being unfairly rewarded (Ritchie, 2016). ProRate resolves both these concerns and ProRate can actually be derived simply from the premise of avoiding “all” cost shifts between ratepayers (Katz, CLEPm Rewards to Arrest Demand Cost-Shifting, 2019; Katz, CLEP5 Rewards to Arrest Energy Cost-Shifting, 2019). Another major problem with the Old Utility Model is the lack of price …

The Pawn System: How Procedurally Adaptive Webbed Narratives Create Stories, Steven T. Bordelon

LSU New Orleans Theses and Dissertations

This thesis describes the design, implementation, and testing of a novel procedural narrative system called the Procedurally Adaptive Webbed Narrative (PAWN) system. PAWN procedurally generates characters and, responding to choices made by the player, produces more responsive characters and relationships involving the player and these narrative agents. Initially, this thesis discusses other interactive narrative types that exist, such as emergent or event-driven narratives, along with their strengths and weaknesses. It then examines each aspect of PAWN, starting with initial actor generation, then moving to the capturing of game events and translating them into logical objects called Occurrences. These Occurrences are …

Towing Tank Trials Of Hydrokinetic Turbine Scale Model To Support Marine Energy System Verification, Shahab Rouhi

LSU New Orleans Theses and Dissertations

In response to the escalating demand for sustainable energy solutions and the critical reevaluation of conventional fossil fuels due to environmental concerns, this dissertation embarks on a comprehensive exploration of hydrokinetic energy as a promising alternative. The study delves into the underexplored domain of hydrokinetic energy, leveraging innovative methodologies for effective utilization and harnessing, particularly through the development and investigation of hydrokinetic turbines.

In the realm of hydrokinetic energy conversion, our research has exclusively concentrated on horizontal-axis turbines, distinct from other turbine configurations. Noteworthy is the adaptation of a conventional horizontal-axis wind turbine for water currents, revealing enhanced performance through …

The Water That Will Be: A Qualitative Look Into The Effects Of Visual Imagery On Participation In Water Conservation Initiatives, Shane Martin White

Graduate Theses and Dissertations

This dissertation explored the role of visual imagery in water conservation initiatives, focusing on its impact on public understanding, emotional connection, and willingness to participate. Drawing from the Theory of Planned Behavior and Affective Disposition Theory, the study investigated how art interventions can enhance comprehension and engagement with water conservation easements. Utilizing qualitative methods, including interviews, focus groups and PhotoVoice, data was collected from stakeholders in the Illinois River Watershed region. The research examined current levels of understanding regarding conservation easements, evaluated the effectiveness of visual imagery interventions in enhancing public awareness, and identified the potential for collaborative approaches between …

5-Year Strategic Direction, 2024–2028: Institute Of Agriculture And Natural Resources, University Of Nebraska–Lincoln, Institute Of Agriculture And Natural Resources University Of Nebraska-Lincoln

Institute of Agriculture and Natural Resources: Institute Publications

IANR: Integrating Learning, Discovery, and Engagement for an Even Better Nebraska

Includes an introduction, description of the components of the Institute of Agriculture and Natural Resources at the University of Nebraska–Lincoln, the IANR pillars, stratgeic aims for 2024–2028, and a section on implementation.

Through The Gendered Language Glass: How Gendered Language Shapes Mental Representations Of Gender And Why It Matters, Emily Roberts

World Languages, Literatures and Cultures Undergraduate Honors Theses

This thesis explores the relationship between gendered-languages and mental perceptions of gender. Compiling various studies that evidence a strong link between gendered language and gender bias, the research creates a framework for discussing the potential gender-equalizing effects of gender-fair language. The paper focuses on the conversation concerning gendered language in France, especially discussing the role of the French Academy in establishing and legitimizing language practices.

Inclusive Policy, Diverse Perspectives: Affirmative Action And The Model Minority Among University Students, Jannie Nguyen

Honors Theses

In lieu of the recent Supreme Court overturn of affirmative action, Asian American students were on the forefront as the main racial group defended in this case. The model minority stereotype obscures societal perceptions of Asians and Asian Americans as collectively intelligent, hard-working, and submissive. Whereas the stereotype appears frequently in academic settings, this research aims to understand and contextualize the perceptions on affirmative action among Asian college students within the context and social dynamics of the model minority stereotype. A qualitative approach was deployed to garner nuance narratives of fifteen participants in the study. Participants in the study represent …

Algorithmic Playgrounds: Investigating The Play Element In Social Media, Ashlyn Daniels

Honors Theses

The integration of digital technology into everyday life has radically changed how people participate in society and culture. Every facet of modern life is shaped by how humans interact with software and this human-computer interaction has directly shaped cultural behaviors and products. Digital tools such as artificial intelligence and social media applications have transformed the common means of cultural production. To better understand how this intertwining of culture and computation impacts modern life, this thesis argues for a better understanding of the relationship between play and computation. Human-technology interactions conceal a more foundational movement of play, and by understanding the …

Summonable Construction Delivery Robot, Kevin M. Lewis

Honors Capstones

In many different construction industries, there is a need for tools, parts, and other necessary items to be transported quickly and efficiently over various types of terrain. Human resources have often been used to address these needs, which can become very time and cost inefficient over long periods. The design proposal here is aimed at addressing this need by developing an autonomous outdoor mobile robot based on a quadrupedal robot design. This approach differs by incorporating a wheeled and quadrupedal hybrid actuation system that provides terrain negotiation and speed at the appropriate times. The team uses Robot Operating System (ROS) …

Great South Metals: Slitter Head Building Process, Nabhan Karim, Evan Swierski, Raven Morin, Patrick Kelsey, Michael Williams

Senior Design Project For Engineers

Great South Metals (GSM) operates two steel slitter machines, which take large coils of flat rolled steel and cut them into smaller strips for use in customers’ applications. Setting up the working components of each machine, called the slitter head, is a precise and arduous task. This system relies on several manually performed calculations and acute attention to detail; and thus, is prone to human error. The management at GSM commissioned our team to simplify the slitter head assembly process by creating an automated computer program and make additional recommendations to improve their operations.

The program we created is a …

Interview With Lee Zyrus Hernandez, Lee Zyrus Hernandez, Diego Rivera

ANTH 4306/6306 Anthropology of Borders

The interview with Lee Zyrus Hernandez explores the potential impact of incorporating Borderlands culture into higher-level mathematics education. Rivera seeks to understand if a practical approach, utilizing the cultural knowledge of Borderlands people, could enhance students' experiences with mathematical courses like college algebra and calculus at the University of Texas Rio Grande Valley (UTRGV). Hernandez, a student with a background in applied statistics and data science, reflects on his experiences with math education, including dual enrollment courses and studies in New York and Atlanta. He discusses the lack of cultural relatability in his math education and expresses support for incorporating …

Interview With Annette Garcia, Annette Garcia, Diego Rivera

ANTH 4306/6306 Anthropology of Borders

Interview with Annette Garcia focusing on experiences with mathematics at a border institution. The interview explores potential impacts of teaching mathematical courses with a practical approach rooted in the culture of the Borderlands people. Annette reflects on her background, multilingualism, academic pursuits, and experiences with math courses, both in high school and college. She discusses the teaching approaches, cultural relevance, and potential benefits of incorporating cultural references into math content. Annette also shares insights on the prospect of math courses being taught in Spanish and proposes a math problem integrating US-Mexico border culture. Overall, the interview provides valuable perspectives on …

Interview With Angelina Gonzalez, Angelina Gonzalez, Diego Rivera

ANTH 4306/6306 Anthropology of Borders

Interview with Angelina Gonzalez exploring the experiences of UTRGV students with mathematics in higher education. Rivera investigates the potential impact of incorporating Borderlands people's funds of knowledge into mathematical courses, moving beyond a purely theoretical approach. Gonzalez reflects on her upbringing in the Rio Grande Valley, her educational journey, and her experiences with mathematics at UTRGV. She discusses the challenges of transitioning from high school to college-level mathematics, noting the disconnect between her cultural background and the content taught in classes. Gonzalez expresses support for math courses that incorporate cultural references, suggesting it would enhance understanding and accessibility for students, …

Archaeal Diversity In The Anna's Hummingbird Microbiome, Lauren E. Chance

Honors Scholar Theses

The microbial communities that are present in and on vertebrates are collectively called the microbiome. The composition of a microbiome is dependent upon the host, the environment, and evolution. There has been extensive research on the bacterial composition of host-associated microbiomes, however, there has been much less work on the archaeal composition of host-associated microbiomes. Archaea have previously been assumed to primarily exist in extreme environments, but this may not be true and has been influenced by their generally low abundance and methodological difficulties in detection. It is possible they are consistent members of diverse host-associated microbiomes.

Archaea-specific PCR primers …

Liquefying The Gulf Coast: A Cumulative Impact Assessment Of Lng Buildout In Louisiana And Texas, Robin K. Saha, Robert D. Bullard, Liza T. Powers

Environmental Studies Faculty Publications

This report represents a substantial endeavor aimed at providing a critical examination of the complex interplay between fossil fuel development, environmental justice, and climate justice in a region of the United States that has an enduring legacy of economic injustice and environmental racism. In recent years, the rapid expansion of liquefied natural gas (LNG) infrastructure along the Gulf Coast has raised significant concerns regarding the environmental and social consequences.

Per- And Polyfluoroalkyl Substance Exposure Risks In Us Carceral Facilities, 2022, Lindsay Poirier, Derrick Salvatore, Phil Brown, Alissa Cordner, Kira Mok, Nicholas Shapiro

Statistical and Data Sciences: Faculty Publications

Objectives. To assess the US incarcerated population’s risk of exposure to per- and polyfluoroalkyl substances (PFASs). Methods. We assessed how many of the 6118 US carceral facilities were located in the same hydrologic unit code watershed boundaries as known or likely locations of PFAS contamination. We conducted geospatial analyses on data aggregated from Environmental Protection Agency databases and a PFAS site tracker in 2022 to model the hydrologically feasible known and presumptive PFAS contamination sites for nearly 2 million incarcerated people. Results. Findings indicate that 5% (∼310) of US carceral facilities have at least 1 known source of PFAS contamination …

Would Access Cavity Design Affect The Mechanical Behavior Of Tooth: A Finite Element Study, Mohamed Ashraf Elhusseini1, Hala Fayek Khalil, Engy M. Kataia

Dentistry

Introduction: Two distinct access cavity designs were used to access Two simulated finite element

models (FE) Lingual (LAC) and incisal (IAC)). Three experimental FE models, including the IT

model, were developed: LAC and IAC.

Methods: For every simulated model, one distinct radicular preparations was carried out of size

#30/.04. The incisal edge was subjected to an occlusal cycle load of 120 N. The maximum von

mises stresses (VMS), maximum principal stresses (MPS), and stress distribution patterns were

assessed and calculated quantitatively.

Results: The traditional access design showed slightly higher fracture resistance to loading than

the conservative incisal access cavity design. …

Magnetic Properties Of Zgnrs With Nitrogen And Fluorine Adsorbates, A Computational Study, Justin Petit

Electronic Theses and Dissertations

Imposing dimensional restrictions on graphene sheets and adding impurities can give rise to carbon nanostructures with magnetic properties. In this work, zigzag graphene nanoribbons, zGNRs, with nitrogen and fluorine adatoms are investigated for magnetic properties of interest for spin devices. Geometry optimizations were done determining which position along a zGNR electrode that N and F would favorably attach to. Edge positions were determined as the most stable attachment site. M-cell zGNR electrodes (M = 1-3) edge-functionalized by N and F adatoms were investigated with respect to their band structures and spin densities in antiferromagnetic and ferromagnetic, AFM and FM, configurations. …

Coastal-Ocean And Estuarine Acidification In Long Bay, South Carolina: A Comparison Of In-Situ Water Quality Monitoring With Measured Carbonate System Parameters, Mary Olsen

Electronic Theses and Dissertations

One of the major water-quality issues impacting our coast and estuaries is coastal-ocean and estuarine acidification. Due to a relative dearth of data in the southeastern United States it is increasingly difficult to determine the full extent and intensity of this problem. This study provides the first characterization of coastal-ocean and estuarine acidification in Long Bay, SC by attempting to leverage long-term water quality data sets at two coastal-ocean pier sites using in-situ YSI EXO sondes deployed at both the surface and bottom waters along with two estuarine sites sampled by volunteer monitors using Orion star multi-meters. Discrete samples (120) …

Gifted And…: Teacher Reflection And Transformation In Response To Teaching Gifted Students, Patrick Gene Rabon

Electronic Theses and Dissertations

12 teachers, representing five schools in a district, shared via individual interviews their insights regarding giftedness and the needs of gifted students. These interviews explored the characteristics of giftedness and gifted education, the needs participants identified through their experiences with their students, the choices participants made regarding these needs, and the various ways participants reflected on these choices. This study used reflexive thematic analysis and transformative learning theory to make sense of the data, and a reparative approach shaped the study’s approach to the collection and analysis of the data. This study found that participants tended to think in terms …

The Elephant In The Classroom: Understanding Teachers’ Implicit Biases In Grading Student Work In A Mathematics Classroom, Tina Marie Medina

Electronic Theses and Dissertations

This study focused on automaticity or the choices we make without conscious thought. Specifically, the study examined how math teachers improved their understanding of the practice and evolution of culturally responsive teaching by analyzing the lived experiences and metacognition of practicing middle school teachers as they assess student work in mathematics. The potential impact of implicit stereotypes, instructor expectations, assessment design, cultural considerations, and other variables on the grading process was investigated. The purpose of this case study was to uncover teachers’ implicit biases as it relates to grading student work in a middle school math class.

This qualitative study …

Tripeptide-Assisted Gold Nanocluster Formation For Fe3+ And Cu2+ Sensing, Jonghae Youn, Peiyuan Kang, Justin Crowe, Caleb Thornsbury, Peter Kim, Zhenpeng Qin, Jiyong Lee

Chemistry Faculty Publications and Presentations

Fluorescent gold nanoclusters (AuNCs) have shown promise as metal ion sensors. Further research into surface ligands is crucial for developing sensors that are both selective and sensitive. Here, we designed simple tripeptides to form fluorescent AuNCs, capitalizing on tyrosine’s reduction capability under alkaline conditions. We investigated tyrosine’s role in both forming AuNCs and sensing metal ions. Two tripeptides, tyrosine–cysteine–tyrosine (YCY) and serine–cysteine–tyrosine (SCY), were used to form AuNCs. YCY peptides produced AuNCs with blue and red fluorescence, while SCY peptides produced blue-emitting AuNCs. The blue fluorescence of YCY- and SCY-AuNCs was selectively quenched by Fe3+ and Cu2+, whereas red-emitting YCY-AuNC …

Public Horticulture: Process And Design Of The Lincoln Botanical Garden, Brad Kindler

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Beginning in 2012, the local non-profit, The Lincoln Botanical Garden, began organizing the conceptual design and creation of a glass conservatory within a botanical garden in Lincoln, Nebraska. These proposed urban enhancements seek to fill a gap in garden amenities in the city by providing year-round access to public horticulture programming and education.

This project, Public Horticulture: Process and Design of the Lincoln Botanical Garden, documents these efforts and makes design recommendations for a themed Glacial Erratic Garden that could be constructed within the botanical garden.

Advisors: Ellen Paparozzi and Sam Wortman

Understanding The Zonal Variability In Cmip6 Projections Of Sahelian Precipitation, Emmanuel Ogwuche Audu

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

The uncertainty in model projections of future precipitation across the Sahel has persisted across many generations of Earth System Models (ESMs), with some models predicting drying and others moistening across this region. These discrepancies in future projections pose a challenge for stakeholders and decision makers. Many projections of Sahel precipitation found in the ESMs participating in the sixth phase of Coupled Model Intercomparison Project (CMIP6) show a zonal dipole in precipitation trend, with moistening across the Central and Eastern Sahel and drying projected for the Western Sahel. Previous studies have connected precipitation variability across the Sahel to changes in various …