Digital Commons Network^™

Effect Of Hypoxic Conditions On Skeletal Myoblasts, Abdo Boumitri, Shuai Zhao

Undergraduate Research Posters 2016

Ischemic injury in skeletal muscle caused by hypoxic (low oxygen) conditions occurs in response to vascular and musculoskeletal traumas, diseases and following reconstructive surgeries. Thus, a thorough understanding of the effect of hypoxia on skeletal myoblasts is warranted to identify potential therapeutic targets. We have determined that treatment with cobalt chloride (to mimic hypoxic conditions) leads to decreased numbers of viable (attached) skeletal myoblast over time and an increase in the percentage of detached myoblasts. To determine the contribution of apoptosis (cell death) to this increase in detached myoblasts, we assessed PARP cleavage, a well- accepted marker for apoptosis. Consistent …

Phosphorus Concentrations In The Cuyahoga River Watershed Linked To Anthropogenic Inputs, Christopher Kasden

Undergraduate Research Posters 2016

Twenty-four water samples were taken from twelve sites throughout the Cuyahoga watershed from upstream to downstream within two separate occasions. Twelve samples were taken on May 25th, 2016 with no prior precipitation and the conditions were calm, and twelve samples were taken on July14, 2016 when high precipitation had occurred and water conditions were much more diverse. Using the Automated Discrete Analyzer, the concentrations of phosphorus could be analyzed and differentiated between Total Phosphorus and Soluble Reactive Phosphorus. In comparison to the Environmental Protection Agency whose target concentrations for rivers is .05ppm, in both cases of the experiment the hypothesis …

Defining An Enriched Environment For Pre-Ambulation Training Using A Multi-Directional, Over-Ground Harness System For Young Children With Down Syndrome, Lauren Schuck, Lisa Haecker

Undergraduate Research Posters 2016

This theoretical literature review aims to define the components of an enriched environment (EE) while using a multi-directional, over-ground harness system (MOH), for pre-ambulatory children with Down syndrome (DS). While using an EE has not been clearly defined within humans, children with DS may benefit from this combination of interventions to optimize developmental outcomes. Of the 15 articles critiqued, 6 were found to be most relevant to help define an EE with specific activities when using a MOH for pre-ambulation training. As a part of defining an EE within a MOH, activities will be suggested that stimulate the four components …

The Effects Of Superior Laryngeal Nerve Lesion On Swallowing Kinematics And Airway Protection, Saja Abid, Rebecca Z. German

Undergraduate Research Posters 2016

The superior laryngeal nerve (SLN) carries sensory information from the mucosal tissues of the pharynx superior to the vocal folds, and carries motor signals to the cricothyroid muscles. It also provides partial innervation to the thyroarytenoid and posterior cricoarytenoid muscles. Finally, the SLN initiates the swallow. When a food or liquid bolus is swallowed, the epiglottis and the false and true vocal folds work together to seal off the airway and allow the bolus to pass through the esophagus. If the SLN is damaged, it usually leads to dysphasia in which food or liquid enters the airway. We hypothesized that …

Correlating Wet-Sample Electron Microscopy With Light Scattering Spectroscopy On The Example Of Polymeric Microgels, Christian Gunder

Undergraduate Research Posters 2016

Amphiphilic cellulose-based microgels with a reversible volume-phase transition at around 40.5°C—the low critical solution temperature (LCST)—have been synthesized, characterized, and optimized. After carefully planned synthesis and filtering the samples with a 0.22μm filter microgels were characterized with dynamic light scattering (DLS), yielding reproducible results for the radii of particles around 100-120 nm below the LCST and 60-70 nm above it. Through the use of scanning electron microscopy (SEM), air dried samples and wet samples were also analyzed. Air dried samples were dried for 24 hours until all water was evaporated, ensuring the collapse of microgels as if they were expelling …

Glycerolipid Analysis Of Adaptation To Saline Changes In The Culture Conditions Of Algae, Scenedesmus Dimorphus, By Gc-Ms, Tyler Fitzgerald, Satya Girish Chandra Avula, Chandana Mannem

Undergraduate Research Posters 2016

Since fossil fuels are decreasing over time an alternative energy source will be soon required. The algae, Scenedesmus dimorphus, grows in freshwater and is known for its fast growth of glycerolipid content which is used for biodiesel production. After the algae is grown in optimal conditions, the released fatty acids and glycerolipids are transformed into fatty acid methyl esters (FAMEs) which are used as biodiesel. The FAMEs were quantitatively determined by gas chromatography mass spectrometry (GC-MS) to determine the total glycerolipid content in the different algae samples. The samples that were analyzed include freshwater controls and saline adapted samples. Analysis …

The Minnesota Farm-Labor Party: The Role Of Third Parties In The Americanization Of European Labor Radicals In The Great Lakes Region, Paul Lubotina

Upper Country: A Journal of the Lake Superior Region

This paper examines the political transformation of former radicals from Ethnic Socialist Parties, Industrial Workers of the World, and the Western Federation of Miners during the 1920s and 1930s, as they joined mainstream third party organizations such as The Nonpartisan League and Farm-Labor Party of Minnesota. Former resident of Copper Harbor, Michigan and mayor of Hibbing, Minnesota, Victor Power led a coalition of Americans and immigrants in a struggle to circumvent the burgeoning power of Ku Klux Klan members who dominated local government in the city, with the support of the United States Steel Corporation. This political battle between progressive …

Unm Pathology Pathfinder Winter 2016, Unm Department Of Pathology

Newsletters

No abstract provided.

Extended Kalman Filter For Stochastic Tool Wear Assessment In Turning Of Inc718 Hard-To-Machine Alloy, Farbod Akhavan Niaki, Martin Michel, Laine Mears

Publications

An Extended Kalman Filter (EKF) is employed in this work for tracking tool flank wear area in wet-turning of Inconel 718 (INC718) Nickel-based alloy in variable feed condition. The tool wear area evolution is modeled with a 3rd order polynomial empirical function and an analytical solution for discrete state space system is derived. The state uncertainty was found to decrease up to 200-250μm of average flank wear length and then increase abruptly with an increase in tool wear. Therefore, the tool wear uncertainty was modeled with failure probability density, i.e. the bathtub function. While a constant uncertainty was considered for …

Live, Attenuated Vaccines And Methods Of Making And Using, Jiantao Guo, Qingsheng Li, Wei Niu, Yue Li, Nanxi Wang

Department of Chemistry: Faculty Publications

A live, attenuated HIV vaccine is provided, and methods of making a atenuated HIV vaccine are provided.

The Treatment Of Six Sigma In Introductory Operations Management Textbooks: Clearing Up The Confusion, Handanhal Ravinder, Ram B. Misra

Department of Management Faculty Scholarship and Creative Works

This paper critically examines the treatment of the statistical basis for Six Sigma and process capability in popular operations management textbooks. It discusses areas of confusion and suggest ways of treating the topic that make sense to instructors as well as students. Even though Six Sigma was introduced almost 30 years ago, misconceptions persist. In the textbooks we have found no consistency of approach or understanding of the statistical underpinnings (3.4 defects per million opportunities) of Six Sigma. Sometimes statements are made that are factually incorrect and cause frustration for students and instructors. Similar difficulties are encountered in discussions of …

Phase Separation In Lufeo3 Films, Shi Cao, Xiaozhe Zhang, Kishan Sinha, Wenbin Wang, Jian Wang, Peter A. Dowben, Xiaoshan Xu

Peter Dowben Publications

The structural transition at about 1000°C, from the hexagonal to the orthorhombic phase of LuFeO₃, has been investigated in thin films of LuFeO₃. Separation of the two structural phases of LuFeO₃ occurs on a length scale of micrometer, as visualized in real space using X-ray photoemission electron microscopy. The results are consistent with X-ray diffraction and atomic force microscopy obtained from LuFeO₃ thin films undergoing the irreversible structural transition from the hexagonal to the orthorhombic phase of LuFeO₃, at elevated temperatures. The sharp phase boundaries between the structural phases are observed to …

Influence Of Steric Hindrance On The Molecular Packing And The Anchoring Of Quinonoid Zwitterions On Gold Surfaces, Minghui Yuan, Iori Tababe, Jean-Marie Bernard- Schaaf, Qin-Yin Shi, Vicki Schlegel, Rachel Schurhammer, Peter A. Dowben, Bernard Doudin, Lucie Routaboul, Pierre Braunstein

Peter Dowben Publications

Driven by the huge potential of engineering the molecular band offset with highly dipolar molecules for improving charge injection into organic electrics, the anchoring of various N-alkyl substituted quinonoid zwitterions of formula C₆H₂ (···NHR)₂ (···O)₂ (R = iPr, Cy, CH₂CH(Et)CH₂CH₂CH₂CH₃,. . .) on gold surfaces is studied. The N–Au interactions result in an orthogonal arrangement of the zwitterions cores with respect to the surface, and stabilize adsorbed compact rows of molecules. IR spectroscopy is used as a straightforward diagnostic tool to validate the presence of …

Children's Mercy Hospital Annual Report 2015, Children's Mercy Hospital

Children's Mercy Annual Reports

Annual report for The Children's Mercy Hospital in Kansas City MO, a pediatric medical center.

Nebraska Water Center Annual Report 2016, Nebraska Water Center

Nebraska Water Center: Administrative Materials

Contents

Foreword

Director’s Letter

Since 1964: The Nebraska Water Center

Building the future

Nebraska’s Top 10 water challenges

Nebraska Water Center advisory board

Water Resources Advisory Panel: A key to success

Nebraska Water Sciences Laboratory

USGS 104b projects for 2016

Ogallala Aquifer focus of USDA research grant

Graduate research on amphetamines draws attention

NIC hosts water symposium and law conference

Water seminar lectures key on “Water and health”

Four picked for IRES program in Czech Republic

WARI program begins

2016 tour to Colorado’s South Platte basin

45th annual tour visits Platte River basin in Colorado

McCornick new Water for Food …

Nebraska Water Center (Brochure ), Nebraska Water Center

Nebraska Water Center: Administrative Materials

The Nebraska Water Center was established in 1964 as one of 54 Water Resources Research Institutes nationwide with passage of the first Clean Water Act. It facilitates the University of Nebraska-Lincoln’s land grant missions in research, extension and teaching in water science, law and policy to address water quantity and quality issues of priority to Nebraska. NWC helps water researchers by awarding small grants, conducting water-related education and outreach activities for stakeholders, and disseminating research results through media, colloquia, conferences, lectures, and tours. NWC provides state-of-the-art analytical technology to conduct water research and education to scientists and engineers, helping them …

Working Trees Info: How Can Agroforestry Help Pollinators?

Working Trees (USDA-NAC)

Over one hundred crop species in North America require a visit from an insect pollinator to be most productive. In the past, native bees, feral honey bees, and other pollinators could meet the needs of these diverse crops because farms were typically interspred with pollinator habitat. Today, farms in the United States are larger and have less nearby habitat to support pollinators.

Private landowners can do a lot to support these critical pollinators by providing habitat and food sources for honey bees, native bees, butterflies, and others. Pollinator habitat and floral diversity on the farm also benefit producers of insect …

Species Discovery And Diversity In Lobocriconema (Criconematidae: Nematoda) And Related Plant-Parasitic Nematodes From North American Ecoregions, Tom Powers, Ernest C. Bernard, T. Harris, Robert Higgins, M. Olson, S. Olson, M. Lodema, Julianne N. Matczyszyn, P. Mullin, L. Sutton, K.S Powers

Department of Statistics: Faculty Publications

There are many nematode species that, following formal description, are seldom mentioned again in the scientific literature. Lobocriconema thornei and L. incrassatum are two such species, described from North American forests, respectively 37 and 49 years ago. In the course of a 3-year nematode biodiversity survey of North American ecoregions, specimens resembling Lobocriconema species appeared in soil samples from both grassland and forested sites. Using a combination of molecular and morphological analyses, together with a set of species delimitation approaches, we have expanded the known range of these species, added to the species descriptions, and discovered a related group of …

Improved Estimation Of Intrinsic Growth RMax For Long-Lived Species: Integrating Matrix Models And Allometry, Peter W. Dillingham, Jeffrey E. Moore, David Fletcher, Enric Cortés, K. Alexandra Curtis, Kelsey C. James, Rebecca L. Lewison

United States Department of Commerce: Staff Publications

Intrinsic population growth rate (r_max) is an important parameter for many ecological applications, such as population risk assessment and harvest management. However, r_max can be a difficult parameter to estimate, particularly for long- lived species, for which appropriate life table data or abundance time series are typically not obtainable. We describe a method for improving estimates of r max for long- lived species by integrating life- history theory (allometric models) and population- specific demographic data (life table models). Broad allometric relationships, such as those between life history traits and body size, have long been recognized by ecologists. …

Precipitation And Growth Of Barite Within Hydrothermal Vent Deposits From The Endeavour Segment, Juan De Fuca Ridge, John William Jamieson, Mark D. Hannington, Margaret K. Tivey, Thor Hansteen, Nicole M.-B. Williamson, Margaret Stewart, Jan Fietzke, David Butterfield, Matthias Frische, Leigh Allen, Brian Cousens, Julia Langer

United States Department of Commerce: Staff Publications

Hydrothermal vent deposits form on the seafloor as a result of cooling and mixing of hot hydrothermal fluids with cold seawater. Amongst the major sulfide and sulfate minerals that are preserved at vent sites, barite (BaSO₄) is unique because it requires the direct mixing of Ba-rich hydrothermal fluid with sulfate-rich seawater in order for precipitation to occur. Because of its extremely low solubility, barite crystals preserve geochemical fingerprints associated with conditions of formation. Here, we present data from petrographic and geochemical analyses of hydrothermal barite from the Endeavour Segment of the Juan de Fuca Ridge, northeast Pacific Ocean, …

Burning Down The Silos: Integrating New Perspectives From The Social Sciences Into Human Behavior In Fire Research, Erica Kuligowski

United States Department of Commerce: Staff Publications

The traditional social science disciplines can provide many benefits to the field of human behavior in fire (HBiF). First, the social sciences delve further into insights only marginally examined by HBiF researchers, in turn, expanding the depth of HBiF research. In this paper, I present examples of studies from the fields of social psychology and sociology that would expand HBiF research into non-engineering or ‘unobservable’ aspects of behavior during a fire event. Second, the social sciences can provide insight into new areas of research; in turn, expanding the scope of HBiF research. In this section, I introduce pre and post-fire …

Alpha-Lipoic Acid Supplementation Protects Enzymes From Damage By Nitrosative And Oxidative Stress, Sylvia Hiller, Robert Dekroon, Eric D. Hamlett, Longquan Xu, Cristina Osorio, Jennifer Robinette, Witold Winnik, Stephen Simington, Nobuyo Maeda, Oscar Alzate, Xianwen Yi

United States Environmental Protection Agency: Publications

Background: S-nitrosylation of mitochondrial enzymes involved in energy transfer under nitrosative stress may result in ATP deficiency. We investigated whether α-lipoic acid, a powerful antioxidant, could alleviate nitrosative stress by regulating S-nitrosylation,which could result in retaining themitochondrial enzyme activity.

Methods: In this study, we have identified the S-nitrosylated forms of subunit 1 of dihydrolipoyllysine succinyltransferase (complex III), and subunit 2 of the α-ketoglutarate dehydrogenase complex by implementing a fluorescence-based differential quantitative proteomics method.

Results: We found that the activities of these two mitochondrial enzymes were partially but reversibly inhibited by S-nitrosylation in cultured endothelial cells, and that their activities …

Estimating Ozone And Secondary Pm2.5 Impacts From Hypothetical Single Source Emissions In The Central And Eastern United States, Kirk R. Baker, Robert A. Kotchenruther, Rynda C. Hudman

United States Environmental Protection Agency: Publications

Secondary pollutant impacts from emissions of single sources may need to be assessed to satisfy a variety of regulatory requirements including the Clean Air Act New Source Review and Prevention of Significant Deterioration programs and the National Environmental Policy Act. In this work, single source impacts on O₃ and secondary PM_2.5 are estimated with annual 2011 photochemical grid model simulations where new hypothetical sources are added to the central and eastern United States with varying precursor emission rates and emission release heights. Impacts from these hypothetical sources are tracked with photochemical grid model source apportionment. Single source impacts …

Seasonality Of Coliform Bacteria Detection Rates In New Jersey Domestic Wells, Thomas B. Atherholt, Nicholas A. Procopio, Sandra M. Goodrow

United States Environmental Protection Agency: Publications

It is important that indicators of fecal pollution are reliable. Coliform bacteria are a commonly used indicator of fecal pollution. As other investigators have reported elsewhere, we observed a seasonal pattern of coliform bacteria detections in domestic wells in New Jersey. Examination of a statewide database of 10 years of water quality data from 93,447 samples, from 78,207 wells, generated during real estate transactions, revealed that coliform bacteria were detected in a higher proportion of wells during warm weather months. Further examination of the seasonal pattern of other data, including well water pH, precipitation, ground and surface water temperatures, surface …

Temporal Trends In The Spatial Distribution Of Impervious Cover Relative To Stream Location, J. Wickham, A. Neale, M. Mehaffey, T. Jarnagin, D. Norton

United States Environmental Protection Agency: Publications

Use of impervious cover is transitioning from an indicator of surface water condition to one that also guides and informs watershed planning and management, including Clean Water Act (33 U.S.C. §1251 et seq.) reporting. Whether it is for understanding surface water condition or planning and management, impervious cover is most commonly expressed as summary measurement (e.g., percentage watershed in impervious cover). We use the National Land Cover Database to estimate impervious cover in the vicinity of surface waters for three time periods (2001, 2006, 2011). We also compare impervious cover in the vicinity of surface waters to watershed summary estimates …

Cyanotoxins In Inland Lakes Of The United States: Occurrence And Potential Recreational Health Risks In The Epa National Lakes Assessment 2007, Keith A. Loftin, Jennifer L. Graham, Elizabeth D. Hillborn, Sarah C. Lehmann, Michael T. Meyer, Julie E. Dietze, Christopher B. Griffith

United States Environmental Protection Agency: Publications

A large nation-wide survey of cyanotoxins (1161 lakes) in the United States (U.S.) was conducted during the EPA National Lakes Assessment 2007. Cyanotoxin data were compared with cyanobacteria abundance- and chlorophyll-based World Health Organization (WHO) thresholds and mouse toxicity data to evaluate potential recreational risks. Cylindrospermopsins, microcystins, and saxitoxins were detected (ELISA) in 4.0, 32, and 7.7% of samples with mean concentrations of 0.56, 3.0, and 0.061 mg/L, respectively (detections only). Co-occurrence of the three cyanotoxin classes was rare (0.32%) when at least one toxin was detected. Cyanobacteria were present and dominant in 98 and 76% of samples, respectively. Potential …

Carbon Storage In Us Wetlands, A. M. Nahlik, M. S. Fennessy

United States Environmental Protection Agency: Publications

Wetland soils contain some of the highest stores of soil carbon in the biosphere. However, there is little understanding of the quantity and distribution of carbon stored in our remaining wetlands or of the potential effects of human disturbance on these stocks. Here we use field data from the 2011 National Wetland Condition Assessment to provide unbiased estimates of soil carbon stocks for wetlands at regional and national scales. We find that wetlands in the conterminous United States store a total of 11.52 PgC, much of which is within soils deeper than 30 cm. Freshwater inland wetlands, in part due …

Psychotherapy Versus Pharmacotherapy For Posttraumatic Stress Disorder: Systemic Review And Meta-Analyses To Determine First-Line Treatments, Daniel J. Lee, Carla W. Schnitzlein, Jonathan P. Wolf, Meena Vythilingam, Ann M. Rasmusson, Charles W. Hoge

Uniformed Services University of the Health Sciences

Background: Current clinical practice guidelines (CPGs) for posttraumatic stress disorder (PTSD) offer contradictory recommendations regarding use of medications or psychotherapy as first-line treatment. Direct head-to-head comparisons are lacking. Methods: Systemic review of Medline, EMBASE, PILOTS, Cochrane Central Register of Controlled Trials, PsycINFO, and Global Health Library was conducted without language restrictions. Randomized clinical trials >8 weeks in duration using structured clinical interview-based outcome measures, active-control conditions (e.g. supportive psychotherapy), and intent-to-treat analysis were selected for analyses. Independent review, data abstraction, and bias assessment were performed using standardized processes. Study outcomes were grouped around conventional follow-up time periods (3, 6, and …

In-Air Hearing Of A Diving Duck: A Comparison Of Psychoacoustic And Auditory Brainstem Response Thresholds, Sara E. Crowell, Alicia M. Wells-Berlin, Ronald E. Therrien, Sally E. Yannuzzi, Catherine E. Carr

United States Geological Survey: Staff Publications

Auditory sensitivity was measured in a species of diving duck that is not often kept in captivity, the lesser scaup. Behavioral (psychoacoustics) and electrophysiological [the auditory brainstem response (ABR)] methods were used to measure in-air auditory sensitivity, and the resulting audiograms were compared. Both approaches yielded audiograms with similar U-shapes and regions of greatest sensitivity (2000-3000 Hz). However, ABR thresholds were higher than psychoacoustic thresholds at all frequencies. This difference was least at the highest frequency tested using both methods (5700 Hz) and greatest at 1000 Hz, where the ABR threshold was 26.8 dB higher than the behavioral measure of …

2016-17 Unopa General Meeting Minutes

UNOPA: Minutes

No abstract provided.