Digital Commons Network^™

Antimicrobial Activity Of Violacein Extracted ​ From Janthinobacterium Lividum​, Catherine M. Brownhill-Slatore

Scripps Senior Theses

Abstract

Antibiotic-resistant bacteria have been a threat since the discovery of antibiotics. The increased rise of antibiotic-resistant bacteria has created an immediate need for new antibiotics. Violacein, a purple bisindole pigment produced by Janthinobacterium lividum, has been shown to be a promising new antibiotic. Its efficacy has not been completely demonstrated, and this project aims to further investigate the effectiveness of violacein as an antibiotic. To do this, a minimum inhibitory concentration (MIC) test was conducted. Violacein was grown, extracted, purified, and tested across concentrations ranging from 1.0 to 500.00 µg/mL to cover values well above and below previously cited …

The Role Of Satirical And Self-Deprecating Humor In Destigmatizing Mental Health In Black Women, Zoe S. Cooper

Scripps Senior Theses

Black humor has long served as a mode of resilience, critique, and emotional expression for Black communities, yet its potential to reduce mental health stigma among Black women remains underexplored. This study examined whether exposure to culturally grounded satirical or self-deprecating humor reduces internalized mental health stigma among Black women and individuals who have experienced Black womanhood. It further investigated whether humor moderates the relationship between endorsement of the Black Superwoman Schema and internalized stigma, a framework closely tied to emotional suppression and stigma in Black women’s mental health. Participants were Black/African American women ages 18–30, as well as individuals …

Beyond Ssris: Omega-3 Fatty Acids And The Neurobiology Of Impulsivity In Borderline Personality Disorder, Sujean Doo

Scripps Senior Theses

Impulsivity is a central and clinically significant dimension of Borderline Personality Disorder (BPD), and is strongly associated with self-harm, aggression, and suicide risk. Despite its prognostic importance, pharmacologic management of impulsivity in BPD remains inconsistent. Selective serotonin reuptake inhibitors (SSRIs) are frequently prescribed; however, randomized controlled trials demonstrate limited efficacy for impulsive-aggression, and some patients experience a paradoxical behavioral activation. Meta-analytic and systematic reviews further suggest that mood stabilizers and second-generation antipsychotics may outperform antidepressants, yet no specific medication is indicated to target impulsive behavioral dyscontrol.

This thesis synthesizes findings from systematic reviews, randomized trials, and neuroimaging research to evaluate …

Remembering Choices: Feedback, Memory Accuracy, And Context-Dependent Preference Shifts, Cameryn K A Okamura

Scripps Senior Theses

Preferences are often assumed to be stable across time and context, yet research on cognition and behavioral economics suggests otherwise. The present study examined whether false reminders of prior choices amplify susceptibility to the asymmetric dominance effect, commonly known as the decoy effect, and secondly, whether working memory capacity moderates this relationship. Twenty-one participants completed an online experiment in which they first chose between pairs of everyday consumer products or services before receiving accurate, inaccurate, or no reminders of their earlier selections. They then made new choices from triplet sets containing a target (T), competitor (C), and asymmetrically dominated decoy …

Improving Maternal Health: Investigating The Early Impacts Of Medicaid Coverage Of Doulas In Oregon, Miriam Akhmetshin

Scripps Senior Theses

While the U.S. spends more per-capita than any country on healthcare, we experience the worst maternal health outcomes of high-income nations. In addition to hefty healthcare bills, an overwhelming majority of mothers experience pregnancy-related health issues that decrease productivity and utility. Thus, it is critical to research cost-effective solutions that can improve maternal health and promote equitable outcomes. Doulas are trained non-medical professionals who support mothers and families before, during and after birth. As a growing body of medical literature points to the role of doulas in decreasing costly medical interventions, insurance companies have begun covering their services. In 2014, …

Large Aerosol Particles Favor Haze Conditions Through Limitations On Water Budget And Activation Kinetics, Hamed F. Sadi, Ernie Lewis, Kadja Flore Gali, Fan Yang, Jesse Anderson, Suryadev Pratap Singh, Jae Min Yeom, Will Cantrell, Raymond Shaw

Michigan Tech Publications

Experiments in the Pi Convection‐Cloud Chamber conducted by systematically changing the diameter of injected dry aerosol particles while holding the temperature difference constant demonstrate that dry diameter strongly influences the onset of haze‐dominated conditions. Two factors contribute: the system becomes water‐limited, resulting in reduction of supersaturation by growing aerosol particles to the activation diameter; and the activation process becomes kinetically limited. Dry aerosol diameter exerts a strong influence on activation time, with a power‐law exponent of 9/2. Kinetically limited activation occurs when the ratio of the activation and droplet residence times is greater than unity. The findings demonstrate that a …

Equity And Justice In Water Pricing And The Effects On Water Distribution System Management, Brian Barkdoll

Michigan Tech Publications

Equity regarding access to potable water is paramount. Municipal Water Distribution Systems supply potable water to users and are essential to public health. To find the optimal price of water to maximize utility revenue and allow changes to the system components for capital and maintenance expenditures, network modeling was performed for 15 real MDWSs. A new power-law price-usage elasticity curve, entitled the Power Law Elasticity Function (PLEF), was used that allowed for various users, both high- and low-income, and a non-discretionary level of usage. It was found that: (1) the maximum profit was obtained when the price was approximately 0.8 …

Use Of A Medication Management Algorithm In Depressed Youth Living With Hiv: Secondary Analysis Of A Multisite Randomized Controlled Trial, Graham J Emslie, Beth D Kennard, Kristin Baltrusaitis, Sophia M Jones, Jessica M Jones, Jaime G Deville, Kathleen Pitts, David E Shapiro, Allison Eliscu, Larry K Brown

Faculty, Staff and Students Publications

Objectives: Depression is a prevalent co-occurring condition in youth living with HIV. This report is a secondary analysis of a multisite cluster randomized trial evaluating the efficacy of a health and wellness cognitive behavioral therapy (CBT) combined with a medication management algorithm utilizing measure-based care (COMB-R) compared with enhanced standard of care (ESC). We describe and compare antidepressant prescribing strategies (i.e., antidepressant treatment, type of antidepressant) for youth living with HIV at COMB-R sites to those at ESC sites over the 24-week intervention. We also describe self-reported adherence to antidepressants.

Methods: Participants (12-24 years) were youth living with HIV diagnosed …

Simulated Oral Environment Affects Zirconia Aging And Mechanical Strength, Danyal A. Siddiqui, Smriti G. Natarajan, Bhuvana L. Chandrashekar, Danieli C. Rodrigues

School of Podiatric Medicine Publications

Background: Zirconia is an emerging alternative to titanium for dental implant systems. However, zirconia surfaces can deteriorate under aqueous conditions, thereby compromising mechanical performance. The goal of this study was to assess the impact of biological exposure or aging simulating the oral cavity on the surface integrity and mechanical strength of surface-treated zirconia.

Methods: Partially yttria- or magnesia-stabilized zirconia specimens were polished, acid-etched, or sandblasted. Zirconia disks were exposed to mammalian cells or bacteria. Surface degradation via tetragonal-to-monoclinic phase transformation was assessed by Raman microscopy. Zirconia bars were subjected to accelerated aging by exposure to autoclave steam. Surface morphology was …

Epigenetic Crosstalk In Neuroblastoma Development And Progression, Nathan Drolet, Amber Wolf, Joanna Yi, Eveline Barbieri

Faculty, Staff and Students Publications

Neuroblastoma (NB), a pediatric malignancy of the peripheral nervous system, accounts for 15% of pediatric cancer deaths and remains a major challenge for cancer therapy. NB originates from sympathoadrenal (SA) progenitors of the neural crest (NC) cell lineage, whose formation and differentiation are controlled by epigenetic mechanisms. Although signaling pathways and epigenetic factors implicated in NB tumorigenesis have been well-described, a holistic understanding of how development and epigenetics overlap to drive NB is lacking. By exploring vital interplays and co-dependencies between key pathways and their epigenetic control in NC development, we have gained novel insights into the mechanisms governing NB …

Case Report: Primed Dendritic Cell Immunotherapy For Multiple Solid Tumors, Jeffrey Jones, Aly Alrabaa, William Decker, Luis Ferbeyre, Gregorio Viramontes, Rafael Gonzalez, Elideth Angulo, Antonio Zamudio, Raul Hernandez, Stephen Noga, Susana Hernandez-Reyes, Paul Zhang, Darryl Smith, Ara Karamanian, Luis Tamara, Dalton Greenwood, Matthew Halpert

Faculty, Staff and Students Publications

Many patients with advanced solid tumors ultimately develop resistance to conventional therapies, with especially limited options for those experiencing rapid progression or poor performance status, highlighting the need for personalized immunotherapies capable of inducing durable responses. In this retrospective case series, we describe the clinical course and outcomes of five adults (4 male, 1 female; median age 51 years [range, 39-62]) with metastatic solid tumors who achieved complete and durable remission following doubly loaded autologous dendritic cell therapy after failure of standard treatments, such as immune checkpoint inhibition, radiation, surgery, or endocrine therapy. Five patients, including prostate adenocarcinoma (n=1), malignant …

Evaluating Mesothelin As An Immunotherapeutic Target For Endogenous T Cells, Kiriakos Koukoulias, Ryu Yanagisawa, Alejandro G Torres Chavez, Penelope G Papayanni, Yovana Velazquez, Hyun-Sung Lee, Spyridoula Vasileiou, Ann M Leen

Faculty, Staff and Students Publications

Background: Mesothelin (MSLN) is a GPI-anchored cell surface glycoprotein that is overexpressed in various solid tumors, including mesothelioma, triple-negative breast cancer, colon, ovarian and pancreatic cancer, with restricted normal tissue expression.

Methods: To explore the immunogenicity and immunotherapeutic potential of MSLN to T cells with native receptor specificity, 29 individuals of diverse HLA backgrounds were interrogated for T cell activity against MSLN.

Results: Twenty one (72%) subjects (21/29) mounted a specific T cell response when repetitively challenged with MSLN antigen. Reactive cells were Th1-polarized, polyfunctional, predominantly detected in the CD8+ T cell compartment and cytotoxic toward autologous and MSLN+/HLA-matched tumor …

Pd-L1-Centric Whole Blood-Based Immune Signature Profiles Of Tuberculosis Patients During Therapy, Johanna Eggeling, Martina Sester, Christoph Lange, Jan Heyckendorf, Barbara Kalsdorf, Anna M Mandalakas, Andrew R Dinardo, David Lewinsohn, Dagmar Schaub, Tina Schmidt, Eva Tolosa, Maja Reimann, Patricia M Sánchez Carballo

Faculty, Staff and Students Publications

This prospective study investigated whole blood-based immune cell biomarkers for pulmonary tuberculosis (TB) immunoprofiling. Blood samples from 34 healthy controls and 51 tuberculosis patients were analyzed at three timepoints: Prior to therapy (T0), after 14 days of therapy (T1), and at the end of treatment (Te). Using multiparameter flow cytometry, 386 immune cell populations were analyzed. Predictive models were developed using two machine learning algorithms. A TB5LF change score, which was based on five cell populations, effectively distinguished tuberculosis patients from controls (AUC = 0.89) and tuberculosis patients before and at the end of treatment (AUC = 0.92). Similarly, the …

Utilizing A Culture System For Horizontal Cells To Study Neural Circuit Assembly In The Developing Mouse Retina, Ross M Perez, Yong H Park, Ajeet Singh, Collin Todora, Marlon F Mattos, Daniela Becerril, Rajashree Venkatraman, Arlene A Hirano, Nicholas C Brecha, Rinki Ratnapriya, Benjamin J Frankfort, Elizabeth Zuniga-Sanchez

Faculty, Staff and Students Publications

The precise wiring of the nervous system relies on neurons extending their processes at the right time and place to find their appropriate synaptic partner. The mechanisms that determine when and where neurons extend their neurites during synaptogenesis remains a central question in the field. In the present study, we used a cell culture system coupled with live imaging to investigate the wiring mechanisms in the developing mouse retina. We focused on horizontal cells which are a class of interneurons in the outer mouse retina known to synapse selectively to the distinct types of photoreceptors. Previous research has shown horizontal …

Using Lotilaner Ophthalmic Solution, 0.25% To Treat Demodex Blepharitis In Patients Who Were Initially Misdiagnosed: A Case Series, Laura M Periman, Richard Lindstrom, Paul Karpecki, Brandon Ayres, Eric Donnenfeld, Marjan Farid, Ian Benjamin Gaddie, Preeya K Gupta, Selina Mcgee, Kelly K Nichols, Stephen Pflugfelder, Christopher E Starr, Nandini Venkateswaran, Elizabeth Yeu, Cecelia Koetting

Faculty, Staff and Students Publications

Introduction: Demodex blepharitis (DB) is a chronic ocular inflammation caused by Demodex mite infestation of the eyelid. DB is often misdiagnosed, as some of its clinical findings and patient-reported outcomes overlap with those of other ocular surface diseases. The Demodex Expert Panel on Treatment and Eyelid Health (DEPTH) has reached consensus on the symptomatology, diagnosis, and treatment of DB. Consensus findings from the DEPTH group include that collarettes are pathognomonic for DB and that lotilaner ophthalmic solution, 0.25%, the first and only FDA-approved treatment for DB, should be the first-line treatment for the disease. This case series highlights two instances …

Non-Congenital Hsv-1 Keratitis In An Immunocompetent 9-Month-Old Infant, Caleb A Casanova, Stephanie Trejo Corona, Stephen F Pflugfelder

Faculty, Staff and Students Publications

Isolated, non-congenital herpes simplex virus type 1 (HSV-1) keratitis in immunocompetent infants is exceedingly rare and may be easily misdiagnosed, leading to delays in appropriate antiviral therapy. This case is unique in that it describes HSV-1 keratitis in a healthy 9-month-old infant presenting without systemic illness, initially treated as bacterial conjunctivitis. The patient presented with unilateral eye pain, redness, and corneal opacification. Subsequent evaluation revealed HSV-1 keratitis, and the patient was treated with intravenous acyclovir in combination with topical moxifloxacin and topical ganciclovir, resulting in complete resolution of symptoms within one week. This case adds to the limited literature on …

Updates In Mycobacterial Keratitis Diagnosis And Treatment, Uma L Balakrishnan, Ryan T Wallace, Patricia Chévez-Barrios, Alejandro Arboleda, Masih U Ahmed

Faculty, Staff and Students Publications

Mycobacterial keratitis is an uncommon but challenging form of infectious keratitis, often associated with postsurgical eyes. Clinical presentation is variable and may be difficult to recognize, resulting in delays in diagnosis and challenges in management. Traditional diagnostic techniques from corneal scrapings are limited by slow organism growth and may yield negative results. Treatment is challenging due to antimicrobial resistance and typically requires prolonged multidrug therapy. Advances in diagnostic modalities, antimicrobial therapies, surgical approaches, and adjunctive treatments such as corneal crosslinking have improved clinicians' ability to mitigate or avoid severe visual consequences. Despite these developments, optimal management strategies remain incompletely defined, …

Common And Recurrent Dysregulated Molecular Network Of Placental Hypoxia And Associated Vasculogenesis And Angiogenesis In Fetal Growth Restriction, Wei Li, Xiaoyi Bai, Oi Ka Chan, Maran Bo Wah Leung, So Ling Lau, Chi Chiu Wang, Tak Yeung Leung

Faculty, Staff and Students Publications

Fetuses with fetal growth restriction (FGR) and selective FGR (sFGR) face elevated health risks both before and after birth. Although the underlying pathomechanisms remain unclear, placental dysfunction is recognized as a major contributing factor. By integrating untargeted transcriptomic data from FGR/sFGR placentae, this study identified 69 differentially expressed mRNAs (DEmRs) and eight differentially expressed miRNAs (DEmiRs). Functional enrichment analysis demonstrated significant enrichment in the angiogenesis and vasculogenesis pathways, with the hypoxia-related genes

The Rise Learning Theory: A New Paths-Way For Teaching Social Emotional Learning Curricula, Rebecca L. Demeo

Scripps Senior Theses

This study aims to look at the effectiveness of the Promoting Alternative THinking Strategies (PATHS) Social Emotional Learning curriculum when taught through the second and fourth principles of the RISE learning theory. The implementation will take effect within United States elementary schools, and consenting students will participate in the quasi-experimental design where classrooms will either be assigned to the control group, PATHS program taught as normal, or the experiment, PATHS taught with the RISE principles. During the study, participants will complete pre-experiment, post-experiment, and follow-up assessments to measure differences in mean scores of competence and application throughout the implementation. I …

"Chatcbt”: Understanding The Implications Of Artificial Intelligence In Cognitive Behavioral Therapy, Esther R. Goldberg

Scripps Senior Theses

The proposed study is intended to explore the impacts of artificial intelligence (AI) tools when used in cognitive behavioral therapy (CBT) to treat anxiety in American adults. The research is designed to measure whether AI systems such as large language models (LLMs) provide sufficient therapy through the factors of empathy, coping skills, and anxiety symptom reduction. Human therapists are hypothesized to surpass chatbots in all three. Through an experimental design, participants will be placed in either an AI therapy condition or human therapy condition. Participants will undergo a 12-week CBT program, after which they will measure anxiety reduction, perceived empathy …

Roots To Recovery: How Therapeutic Horticulture Can Heal Sexual Trauma, Fiona E. Larsen-Teskey

Scripps Senior Theses

Sexual assault is one of the most prevalent sources of trauma among females. While still requiring more research, therapeutic horticulture (TH), also known as gardening therapy, has so far shown promising results regarding trauma recovery. In this study, I seek to discover how TH can aid in female sexual assault recovery. To determine its impact, I will conduct a three-month experiment with two groups: those who receive cognitive behavioral therapy (CBT) and those who receive CBT plus TH. I will specifically measure three common side effects survivors face: dissociation, shame, and PTSD. These categories will be measured through surveys and …

Pervasive Policing: The Psychological Effects Of Overcriminalization Of Black And Latino Youth, Chloe Gonzalez

Scripps Senior Theses

Recent years have brought increased awareness to the discriminatory treatment of Black and Latino individuals by the police, but a gap in the literature remains at the intersection of the study of criminalization and psychology. The proposed study will attempt to bridge this gap by investigating the extent to which the overcriminalization of Black and Latino high schoolers contributes to the internalization of racialized stereotypes and the development of depressive symptoms. The research will implement a correlational approach combining quantitative and qualitative methods to gather data from a sample of Black and Latino high schoolers ages 14 to 18. Participants …

Art Therapy’S Effect On The Social And Emotional Skills Of Children With Autism: A Naturalized Parent Mediated Design, Abigail Adell

Scripps Senior Theses

This study examines the effect of art therapy on the social and emotional skills of children with autism. Using sensitive design and language, participants will take the CARS2 and ADOS-2 and then undergo an eight-week art therapy program. Families will attend weekly training sessions at the autism center their child already attends for eight consecutive weeks, with each session lasting approximately 90 minutes. Parents will implement the assigned art activity at home five times per week between training sessions, with each home session lasting fifteen to twenty minutes. The program uses a naturalized parent-mediated design so topics and projects can …

Boredom And Creativity: The Effects Of Unstructured Play Versus Screen Time On Creative Performance, Vivienne J. Swenson

Scripps Senior Theses

Children today spend increasing amounts of time engaged with digital media, raising questions about how screens may displace developmental experiences that support creativity. Although research suggests that unstructured play and even mild boredom can facilitate divergent thinking, few studies have directly compared unstructured, self-directed play with passive screen use in early childhood. The present study proposes an experimental design to examine whether 20 minutes of unstructured play leads to higher creativity scores on the Thinking Creatively in Action and Movement (TCAM) than an equivalent period of passive screen engagement among children ages 3–5. Using matched-group assignment based on demographic, cognitive, …

Bilingual Beginnings: Expressive Language And Socio-Emotional Development In Early Childhood, Molly C. Mccormick

Scripps Senior Theses

Although research on bilingualism has expanded rapidly in recent years, the majority focuses on cognitive advantages or receptive vocabulary, or the words children understand. Far less attention has been given to how expressive language, the words children produce, and its potential role as the mechanism linking bilingual exposure to socio-emotional development. Although dual-language learners sometimes appear to have relatively limited expressive vocabularies in one language, these differences tend to reflect patterns of language use and input quality rather than true developmental delays. Even so, temporary differences in expressive skills can be especially meaningful during children’s formative years, when they rely …

Resilience As A Pathway From Openness To Eustress In College Students, Karis H. Choi

Scripps Senior Theses

College students experience high levels of stress, yet not all stress leads to negative outcomes. This study examines how personality and coping resources contribute to eustress—a positive, motivating form of stress—by investigating whether resilience mediates the relationship between openness to experience and eustress. Undergraduate participants completed baseline measures of openness and resilience before engaging in either a high-demand mental arithmetic task or a low-demand control task. Following the task, participants reported their emotional and behavioral responses using the Measure of Emotional, Physical, and Behavioral Markers of Eustress and Distress (MEDS). Consistent with expectations, individuals higher in openness were predicted to …

Exploring The Moderating Role Of Ethnic Identity In Asian American Multi-Ethnic Youths’ Sense Of Purpose And Life Satisfaction, Grace Cheung

Scripps Senior Theses

The rapid increase of interracial and interethnic families globally creates a need for researchers to understand the unique experiences and development of multi-ethnic youths’ sense of identity and purpose as they relate to well-being. Emerging research in Asian American psychology also suggests the urgency and need to reexamine the experiences of individual ethnic groups. The current study will examine the moderating role of ethnic identity in Asian American multi-ethnic youths’ sense of purpose and life satisfaction through a mixed methods approach that includes an online survey followed by an interview for a subgroup of participants. Anticipated results will show how …

An Analysis Of Eating Disorders As A Comorbidity Of Autoimmune Diseases, Madeleine Shore

Scripps Senior Theses

For many autoimmune disease patients, psychiatric conditions are a common comorbidity. Although there are many shared mechanisms between autoimmune disease and psychiatric conditions, current research surrounding mental health among autoimmune disease patients often focuses on mood disorders rather than eating disorders. Despite the high risk for developing psychiatric conditions, most autoimmune disease patients are not screened for eating disorders. This lack of screening is significant, because if an eating disorder is missed by a healthcare provider, the autoimmune or eating disorder condition could be exacerbated, resulting in severe illness or even fatality. Given an existing link between comorbid psychiatric disorders, …

Effects Of Teacher Training On Identifying Inattentive Type Adhd In Afab Students, Gabrielle A. Boles

Scripps Senior Theses

Teachers are more likely to detect hyperactive ADHD symptoms than inattentive ones, which often results in students, especially girls with inattentive symptoms, being under-referred (Willcut, 2012). This study evaluates whether a targeted, inattentive-focused, and gender responsive teacher training reduces gendered and symptom-based biases in referral decisions. K-5 teachers will be randomly assigned to receive either standard general ADHD identification training or an inattentive-focused, gender-responsive training, and will then be asked to review vignettes depicting children with hyperactive and inattentive symptoms, with varying student genders. Referral accuracy and knowledge of ADHD will be measured. Training condition, symptom presentation, and student gender …

Assessing Diagnostic Overshadowing For Co-Occurring Autism Spectrum Disorder (Asd) And Eating Disorders (Ed), Eden Sweet

Scripps Senior Theses

Significant behavioral overlaps in feeding patterns are observed among individuals with eating disorders (EDs) and autism spectrum disorder (ASD). These behavioral similarities can blur diagnostic boundaries, contribute to diagnostic overshadowing, and consequently increase the risk of misdiagnosis and the implementation of ineffective treatment approaches. The proposed study addresses the risk of diagnostic overshadowing as it relates to the ambiguous distinction between autism-driven feeding behaviors and clinically significant eating disorders (ED) within the autistic population. Although the literature has acknowledged behavioral similarities between ASD and EDs, limited research has systematically examined how they impact diagnostic practices and influence the likelihood of …