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Role Of The Small Intestinal Microbiota In The Pathogenesis Of Environmental Enteric Dysfunction In Undernourished Children And Mothers, Clara Kao

Arts & Sciences Graduate Student Theses and Dissertations

Undernutrition in women and its propagation in their children remains a major global health challenge. Worldwide, one in five children under five years of age manifests undernutrition as impaired linear growth, or stunting. Stunting, defined as having a length-for-age z-score (LAZ) <-2, is associated with poor developmental outcomes, including impaired neurodevelopment and immune responses. Furthermore, stunting and its long-term sequelae persist across generations, contributing to an intergenerational cycle of undernutrition. Existing nutritional interventions fail to substantially improve stunting, highlighting the need to (i) better understand other factors contributing to stunting and (ii) focus clinical efforts towards treating maternal undernutrition. Environmental enteric dysfunction (EED) is a subclinical inflammatory enteropathy of the small intestine proposed to contribute to stunting. EED is diagnosed histologically by blunted intestinal villi, epithelial barrier disruption and an immunoinflammatory infiltrate in the lamina propria. However, the pathophysiology of EED remains poorly defined. Furthermore, the small intestinal microbiota remains understudied due to difficulties in sampling. Evidence that the small intestinal microbiota contributes to EED pathogenesis comes in part from our collaborative work with colleagues at icddr,b (the International Centre for Diarrhoeal Disease Research, Bangladesh) who oversaw the Bangladesh EED (BEED) study. In this study children, aged 12-18 months residing in an urban slum in the Mirpur district of Dhaka who failed a nutritional intervention for their stunting, underwent esophago-gastroduodenoscopy (EGD) to obtain duodenal mucosal biopsies, aspirates of duodenal luminal fluid and plasma samples. A set of 14 bacteria taxa (defined by 16S rRNA amplicon sequencing) were identified in the duodenal microbiota of >80% of children with EED. Moreover, the absolute abundances of these ‘core taxa’ were positively correlated with duodenal mucosal and systemic inflammation in study participants and negatively correlated with linear growth. Our lab showed that introduction of a consortium of bacteria cultured from these duodenal aspirates, including these core taxa, produced small intestinal inflammation in young adult gnotobiotic, providing …

Editorial–Special Issue On Application Of Cold Atmospheric Plasma In Human And Veterinary Disease, Katharina Stapelmann, Eloisa Sardella, Vandana Miller

Kimmel Cancer Center Papers, Presentations, and Grand Rounds

Plasma medicine is a rapidly evolving interdisciplinary field that combines plasma science and engineering, chemistry, biology, and medicine. This special issue focuses on various ways to apply cold atmospheric pressure plasma in translating plasma technology into clinical applications. Ongoing research on the redox-based mechanism of plasma action, the concept of 'plasma dose' and device control is critical for advancing plasma medicine. As these applications move toward the clinic, ensuring the reproducibility and quality control of plasma sources is essential. The articles in this issue present examples of both direct and indirect plasma applications, with indirect treatments describing cell exposure to …

Streptococcus Pneumoniae Cervical Abscess In A Pcv-Immunized Female With Waldenstrom's Macroglobulinema: Exploring The Role Of Opsosonization Failure And Functional Hyposplenesim Beyond The Spectrum Of Malignant B-Cell Disorders, Tanya Amal, Paul Johnson, Ayesha Sharif, Usman Bin Hameed

Conference Presentation Abstracts

A 64-year-old female with recently diagnosed Waldenström's macroglobulinemia presented with pain and swelling in the neck. Three weeks prior, she had accidentally punctured her neck with her fingernail, leading to initial swelling. She visited the ER, but workup showed no acute infection. A contrast-enhanced CT scan revealed enlarged bilateral cervical lymph nodes. Over the next two weeks, the swelling became increasingly painful. On presentation, she had sharp, localized pain (10/10 intensity), tachycardia, and a 3x3 cm swelling on the right side of her neck, with redness, warmth, and tenderness. Laboratory results showed lymphocytic leukocytosis (WBC 31.5, lymphocyte 24.8%, neutrophil 6.1%). …

The Diagnostic Dilemma Of Multisystemic Vasculitides - A Case Report Of Granulomatosis With Polyangiitis, Muhammed Hussain, Tabssum Chauhan, Hafsa Hassan, Marian Jamil, Cecilia Big, Rajiv John

Conference Presentation Abstracts

Granulomatosis with Polyangiitis (GPA) is a small vessel vasculitis disorder that classically involves the upper and lower respiratory tract as well as the kidneys. However, its presentation is not limited to these organ systems as it can present with ocular, dermatological or cardiac involvement, thus creating a broad differential when evaluating patients. This case illustrates the diagnostic challenges with infectious and inflammatory etiologies in a patient with systemic symptoms, concerning imaging and travel to a TB-endemic region. A 57-year-old female with a pertinent history of recurrent sinusitis, left otitis media, and prior sinus surgeries was evaluated for suspicion of possible …

Septic Arthritis Caused By A Rare Yeast, Lodderomyces Elongisporus, Osman Muslehuddin, Daphne Pete, Husam Muslehuddin, Hafeezuddin Ahmed

Conference Presentation Abstracts

Introduction: We present a rare case of septic arthritis caused by Lodderomyces elongisporus, a yeast rarely implicated in human infections. This case highlights the diagnostic challenges and management strategies for fungal septic arthritis in an immunocompromised patient. Case Presentation: A 63-year-old African American woman with a complex medical history, including chronic heart failure (CHF), hypertension, end-stage renal disease (ESRD) on hemodialysis, severe protein-energy malnutrition on total parenteral nutrition, duodenal switch with malabsorption, deep vein thrombosis (DVT) on warfarin, and previous sepsis episodes, presented with worsening dyspnea. She was admitted in August 2024 for acute-on-chronic CHF exacerbation. During her admission, she …

Clinician Comfort, Attitudes, And Practices With Provision Of Harm Reduction Supplies To Patients Who Use Drugs, Amanda Cahn, Melissa Calica, Erin Mooz, Malia Haddock, Wendy Craig, Tania Strout, Kinna Thakarar

Costas T. Lambrew Research Retreat 2025

Over 100,000 individuals in the United States died from drug overdose in 2022, over 80,000 of which involved opioids. Overdose deaths from psychostimulants (cocaine, methamphetamine) have also been on the rise.

There has also been an increase in substance-related emergency department visits and hospitalizations. People who use drugs (PWUD) often have longer hospital stays, higher self-discharge, and readmission rates.

Infective Endocarditis In The Overdose Crisis: Addiction Care And Healthcare Utilization, Eva J. Farkas, Victoria Molina, Brittany Mohoney, Wendy Craig, Amy Mcauliffe, Kinna Thakarar

Costas T. Lambrew Research Retreat 2025

What is the impact of the IMAT program on healthcare utilization and addiction care outcomes following admissions for IDU-IE?

Shortage As A Catalyst For High-Value Care: Evaluation Of A Bloof Culture Stewardship Intervention Driven By Supply Chain Disruption, Lesley B. Gordon, Camille Ezran, Elizabeth Herrle, Christina Yen, Nicholas J. Mercuro, Daniel J. Diekema

Costas T. Lambrew Research Retreat 2025

Evaluate the impact of diagnostic stewardship interventions on repeat blood culture ordering in bacteremic patients during the 2024 BD Bactec™ blood culture bottle shortage.

Healthcare-Associated Infections Secondary To Multidrug-Resistant Organisms In Hospitalised Patients With Covid-19 Pneumonia: A Single-Centre Experience, Yahyia Al Hanashi, Abdullah Balkhair

Sultan Qaboos University Medical Journal

Objectives: Hospitalisations related to COVID-19 pneumonia have increased the risk of healthcare-associated infections (HCAIs), including those caused by multidrug-resistant organisms (MDROs). This study aimed to investigate the epidemiology of HCAIs secondary to MDROs among hospitalised patients with COVID-19 pneumonia. Methods: This retrospective study population included patients with COVID-19 pneumonia aged 18 years and older who were admitted to Sultan Qaboos University Hospital, Muscat, Oman, for more than 48 hours between March 2020 and December 2021. Results: A total of 486 patients were included in this study. Among the cohort, 76 patients (15.6%) were diagnosed with concurrent HCAIs …

Real-World Clinical Burden Of Patients Presenting With Vaginitis Symptoms In The United States, Justin Chen, Jenny Tse, Liucheng Shi, Mindy M. Cheng, Rebecca Lillis, Aimee M. Near

School of Medicine Faculty Publications

Background: While vaginitis is a leading cause of primary care visits among women with a gynecologic-related diagnosis, there are limited contemporary data on the healthcare burden. This study describes the real-world healthcare resource utilization (HCRU) of patients presenting with vaginitis symptoms in the United States (US) at symptom presentation and over long-term follow-up. Methods: This retrospective study utilized IQVIA's Longitudinal Prescription (LRx) and Medical Claims (Dx) databases to capture patients presenting with vaginitis symptoms from January 1, 2018 to September 30, 2022. The date of the first diagnosis code for vaginitis or related symptoms was considered the first clinical presentation …

Staphylococcal Scalded Skin Syndrome (Ssss): A Short Review, Anmar M. K. Al-Maamori, Zahraa M. Altaee, Noor Mahommoud Naj, Rafflaa S. Hussien

Hilla University College Journal For Medical Science

Staphylococcal scalded skin syndrome (SSSS) is a skin problem characterized by the formation of blisters. It primarily affects newborns, young children, and adults with pre-existing medical conditions. This condition is caused by an infection from the bacteria Staphylococcus aureus. The cause of this case is the breakdown of desmoglein-1, a protein in the skin, due to the action of exotoxins. This results in the outer layer of the skin's cells peeling off. The SSSS is caused by the dissemination of toxins throughout the body, leading to a widespread rash and a severe manifestation of symptoms. Staphylococcal scalded skin syndrome …

Staff Education For Infection Control Prevention, Beatrice I. Cesar

Walden Dissertations and Doctoral Studies

This project was a quality improvement staff education program to address the increase in hospital-acquired infections in the project facility. The hospital administrator identified that staff were overwhelmed and may not be consistently implementing appropriate infection control practices. If infection control practices are not addressed in hospitals system, there would be a much higher rate of hospital-acquired diseases among hospitalized patients and the staff. A staff education program about evidence-based strategies relating to infection control prevention was implemented to increase the nurses’ knowledge about strategies to decrease the spread of infection in the project facility. Eleven registered nurses participated in …

Conflict, Human Development, And Neonatal Health: A Regional Analysis Of The Eastern Mediterranean, Zynub A. Al-Sherri

Honors Theses

Background. The effects of war and conflict on a population are direct and indirect in nature. One of the most devastating consequences is the destruction of healthcare infrastructure, which severely disrupts access to essential services and impedes long-term socioeconomic development. Among those most affected are newborns, as the neonatal period represents the most vulnerable stage of a child’s life and serves as a sensitive indicator of a society’s overall health and socioeconomic conditions. Given the persistent instability in the Eastern Mediterranean Region (EMR), this study aimed to explore how conflict and human development are associated with neonatal health outcomes …

Parental Vaccine Hesitancy And The Decision To Vaccinate Children Against Covid-19, Jessica Mckee

Walden Dissertations and Doctoral Studies

While there is a significant body of literature regarding vaccine hesitancy, there is a gap in the literature on its impact to parents’ decisions to vaccinate their children against a pandemic pathogen. The purpose of this study was to examine the relationship between parental vaccine hesitancy, measured using the health belief model (HBM) theoretical framework constructs of the Vaccine Hesitancy Scale (VHS; the degree to which an individual trusts in vaccine efficacy, safety, the reliability and trustworthiness of vaccine programs, community impact, perceived risks, and the importance of childhood vaccinations), and parents’ decision to vaccinate their children against COVID-19 with …

Covid 19 And Its Widespread Effects On Autoimmune Disease, Jessica Ngo, Michelle Jankowski, Matthew Sims, Dwayne Baxa

Conference Presentation Abstracts

COVID-19 has disproportionately affected racial and ethnic minority groups and immunocompromised groups, including those with autoimmune diseases. Studies have shown that African American, Hispanic, and Asian American individuals have higher risk of COVID-19 positivity and ICU admissions. SARS-CoV-2 shares numerous similarities with autoimmune diseases in its symptomology and ability to dysregulate the immune response. This study seeks to investigate differences in COVID-related outcomes between patients with autoimmune diseases and the general population. Methods A multi-center retrospective study was conducted with data from the EPIC database at Corewell Health System in Michigan from December 2019 to November 2021. Variables collected included …

The Silent Progression Of Otomastoiditis: A Case Report On Skull Base Osteomyelitis And Cerebral Venous Thrombosis, Tabssum Chauhan, Ammara Aftab, Ahamed Abdulbagi, Wafa Ahmed, Rajiv John

Conference Presentation Abstracts

Introduction Otomastoiditis, a common ear infection, can progress to severe complications such as skull base osteomyelitis (SBO) and cerebral venous thrombosis (CVT), though these are rare. The incidence of CVT in the context of otomastoiditis is approximately 3 to 4 cases per million adults, but it remains life-threatening. Pre antibiotics, CVT secondary to otomastoiditis showed a near100% mortality rate. Today, early recognition and multidisciplinary management make these complications preventable. This case raises the importance of in-time diagnosis and management in averting severe outcomes such as neurological deficits and death. Case Report A 78-year-old female with uncontrolled insulin-dependent diabetes (HbA1c 10.3%) …

Development Of Parapneumonic Effusion In A Patient Using Kratom, Emma Glover, Morgan Kiryakoza, Nader Mina

Conference Presentation Abstracts

Introduction: “Kratom” refers to both Mitragyna speciosa, a tree native to Southeast Asia, and to its products, derived from the tree’s leaves, which are marked as an herbal supplement. Kratom is an herbal supplement with both opioid and stimulant-like effects. While there are no approved uses for kratom by the U.S. Food and Drug Administration, it is frequently used by individuals to manage withdrawal symptoms and cravings (especially related to opioid use), pain, fatigue, and mental health disorders. Furthermore, because kratom is unregulated, it can contain contaminants, including heavy metals and harmful bacteria. This case details the development of parapneumonic …

Artificial Intelligence In Infectious Diseases: Hopes And Hurdles, Hawra Al-Lawati, Abdullah Balkhair

Sultan Qaboos University Medical Journal

No abstract provided.

Epidemiology, Risk Factors And Clinical Outcome Of Candidaemia: A Decade-Long Experience At A University Hospital In Oman, Tasneem Al Salmani, Asmaa S. Mahdi, Abdullah Balkhair, Turkiya Al Siyabi, Hashim Ba Wazir

Sultan Qaboos University Medical Journal

Objectives: This study aimed to examine a decade-long epidemiology of candidaemia to explore the risk factors associated with it, and describe its clinical outcomes in hospitalised adult patients. Methods: This retrospective observational study was conducted at Sultan Qaboos University Hospital, Muscat, Oman. Adult patients with candidaemia hospitalised between January 2007 and December 2016 were included. The hospital infection control department surveillance registry on bloodstream infections (BSIs) was used to identify Candida BSIs. Patients' electronic medical records were searched for demographic characteristics, clinical risk factors and outcomes. Results: Candida albicans was the most common Candida species among 152 …

Artificial Intelligence-Based Virtual Staining Platform For Identifying Tumor-Associated Macrophages From Hematoxylin And Eosin-Stained Images, Arpit Aggarwal, Mayukhmala Jana, Amritpal Singh, Tanmoy Dam, Himanshu Maurya, Tilak Pathak, Sandra Orsulic, Kailin Yang, Deborah Chute, Justin A Bishop, Farhoud Faraji, Wade M Thorstad, Shlomo Koyfman, Scott Steward, Qiuying Shi, Vlad Sandulache, Nabil F Saba, James S Lewis, Germán Corredor, Anant Madabhushi

Faculty, Staff and Students Publications

Background: Virtual staining is an artificial intelligence-based approach that transforms pathology images between stain types, such as hematoxylin and eosin (H&E) to immunohistochemistry (IHC), providing a tissue-preserving and efficient alternative to traditional IHC staining. However, existing methods for translating H&E to virtual IHC often fail to generate images of sufficient quality for accurately delineating cell nuclei and IHC+ regions. To address these limitations, we introduce VISTA, an artificial intelligence-based virtual staining platform designed to translate H&E into virtual IHC.

Methods: We applied VISTA to identify M2-subtype tumor-associated macrophages (M2-TAMs) in H&E images from 968 patients with HPV+ oropharyngeal squamous cell …

Excess Fructose In High-Fructose Corn Syrup Reduces Cell Viability & Promotes Nitric Oxide Release In Raw 264.7 Macrophages, Renzo F. Galarza, Edward L. Villegas

Research and Creativity Symposium

High-fructose corn syrup (HFCS) is an artificial sweetener that contains slightly higher concentrations of fructose compared to standard sucrose. It has been linked to an increased risk of obesity, type 2 diabetes, cardiovascular disease, and fatty liver disease. While both HFCS and sucrose are widely used sweeteners, growing evidence suggests they may differ in their inflammatory potential. We sought to understand the effects of elevated fructose on RAW 264.7 macrophages. To investigate these effects, we treated the cells with either a sucrose solution or a
solution designed to mimic high-fructose corn syrup. Lipopolysaccharide (LPS) stimulation was used to model innate …

Icipe Monthly Digest – Volume 1, Issue 5, May 2025, International Centre Of Insect Physiology And Ecology (Icipe)

icipe Newsletters

No abstract provided.

Unraveling The Antiviral Mechanisms Of Bat Cells: Processing Of Genomic Rnas By Dicer In Bat Cells Limits Sars-Cov-2 Replication, Iyanuoluwani Owolabi

Dissertations

Bats are reservoirs for numerous viruses that cause serious diseases in other animals and humans. Several mechanisms are proposed to contribute to the tolerance of bats to these pathogens. This study investigates the response of bat cells to double-stranded RNA generated by SARS-CoV-2 replication. Here, we found the involvement of Dicer in the processing of viral genomic RNAs during SARS-CoV-2 infection. Examining RNA sequencing of infected cells, small-interfering RNA (siRNA)-like fragments were found derived from viral RNAs. Depletion of Dicer showed a reduction in these RNAs and an increase in viral loads suggesting unlike other mammals, bats may use Dicer …

Pseudomonas Aeruginosa Exoy Enzymatic Activity Inhibits Exos-Induced Caspase-3/7 Activation But Not Cytotoxicity In Pmvecs, Connor Holm

Poster Presentations

Pseudomonas aeruginosa (P. aer.) is the most common cause of ventilator associated pneumonia (VAP) in ICU patients. Different combinations of exoenzymes (S, T, Y, and U) are found in P. aer. strains. ExoY is most often associated with VAP in ICU patients. ExoY infection of pulmonary microvascular endothelial cells (PMVECs) induces cell rounding but does not activate intracellular caspase-3/7 or cause cell death. ExoS and ExoT infection of epithelial cells leads to caspase-3/7 dependent cell death. Previous studies using epithelial cells show that ExoY alleviates the cytotoxic effects of ExoS and ExoT. Here, we sought to determine whether ExoY inhibits …

Modern Concepts Of Health And Disease: An Introduction For Public Health Students, Rose Grace Grose

Open Course Materials

This course outline for Modern Concepts of Health and Disease provides a comprehensive framework for teaching foundational public health concepts to undergraduate students in an in-person setting, with flexibility for adaptation to online or hybrid formats. This outline is specifically designed to reduce financial barriers for students using Open Educational Resources (OER) and other free-to-students materials, ensuring equitable access to high-quality learning materials without the cost of traditional textbooks. The course is designed for a 15-week semester, divided into seven Modules. The course syllabus is available on request.

The assigned readings and videos were carefully curated to reflect diverse, evidence-based …

Investigating Regulation Of Neutrophil-Mediated Inflammation In Mycoplasma Pneumoniae Infection, Nathan D. Velazquez

University Scholar Projects

No vaccine exists for Mycoplasma pneumoniae (Mp) infections, largely due to the

gap in understanding in the virulence and immunopathogenesis of this bacterium.

We have recently demonstrated that neutrophils are maladaptive in the context of

Mp infection, and drive disease severity while also impairing bacterial clearance.

Furthermore, we observed that B cells play a regulatory role in limiting disease

severity by modulating neutrophilic inflammation. We investigated this

phenomenon further to elucidate the mechanisms by which B cells regulate

neutrophilic inflammation. We utilized a monoclonal antibody to deplete B cells

during Mp infection and found that B-cell-depleted mice had elevated neutrophil …

Frontier Healthscapes: Competing Narratives Of Disease, Environment, And Wellbeing In Early Minnesota, Aidan Reynolds

Geography Honors Projects

Across settler societies, health narratives were shaped by evolving tensions between lay and expert knowledge, frameworks of disease causation, and perceptions of the environment as both a source of health and a threat. In the decades following Euro-American settlement (1860-1900), Minnesota became a focal point for these debates, with its landscape fostering complex and competing narratives on health, wellbeing, and disease. This paper explores how public health discourse developed in Minnesota through the perspectives of institutions, the public, and economic interests. Combining environmental history with critical discourse analysis of archival materials, this study examines shifts within the Minnesota State Board …

California’S Ban On Cruel Or Unusual Punishment: A State Constitutional Analysis Of Anti-Camping Ordinances, Anna R. Janson

Connecticut Law Review

In Martin v. City of Boise, the United States Court of Appeals for the Ninth Circuit relied on the Cruel and Unusual Punishment Clause of the Eighth Amendment to rule that a class of involuntarily unhoused individuals may not be criminally punished for sleeping on public property in the absence of “sufficient alternatives” for all unhoused people. In Johnson v. City of Grants Pass, the Ninth Circuit elaborated that civil schemes which lead to criminal punishment are unconstitutional as well. However, the United States Supreme Court granted certiorari in the latter case and disrupted both Ninth Circuit decisions. The Court …

Genomic Analysis Of The Lyme Disease Vector Across Virginia’S Spatial Expansion Front, Amy Jablonski

Honors Theses

Lyme disease is the most common vector-borne disease in the United States and is expanding with rising incidence in Virginia . This study investigates populations of lxodes scapularis across Virginia using RAD-seq to analyze SNPs from ticks collected in seven Virginia counties and four U.S. states as references . Results show a clear genetic split between western and eastern Virginia ticks . These results follow previously noted patterns in regional differences in Lyme disease risk.

Bard Observer, Vol. 23. No. 6 (May 2025), Bard College

Bard Observer, 2020 - 2029

No abstract provided.