Digital Commons Network^™

Biomolecular Condensates In Immune Cell Fate, Srikanth Kodali, Caroline M Sands, Lei Guo, Yun Huang, Bruno Di Stefano

Faculty, Staff and Students Publications

Fate decisions during immune cell development require temporally precise changes in gene expression. Evidence suggests that the dynamic modulation of these changes is associated with the formation of diverse, membrane-less nucleoprotein assemblies that are termed biomolecular condensates. These condensates are thought to orchestrate fate-determining transcriptional and post-transcriptional processes by locally and transiently concentrating DNA or RNA molecules alongside their regulatory proteins. Findings have established a link between condensate formation and the gene regulatory networks that ensure the proper development of immune cells. Conversely, condensate dysregulation has been linked to impaired immune cell fates, including ageing and malignant transformation. This Review …

Dermatologic Fungal Neglected Tropical Diseases-Part I Epidemiology And Clinical Features, Kaya L Curtis, Jeremy A W Gold, Jana M Ritter, Theodore Rosen, Daniel Wagner C L Santos, Dallas J Smith, Shari R Lipner

Faculty, Staff and Students Publications

In this part 1 of a 2-part continuing medical education series, the epidemiology, clinical features, and diagnostic methods for fungal skin neglected tropical diseases (NTDs), which include eumycetoma, chromoblastomycosis, paracoccidioidomycosis, sporotrichosis, emergomycosis, talaromycosis, and lobomycosis, are reviewed. These infections, several of which are officially designated as NTDs by the World Health Organization, cause substantial morbidity and stigma worldwide and are receiving increased attention due to the potential for climate change-related geographic expansion. Domestic incidence may be increasing in the setting of global travel and immunosuppression. United States dermatologists may play a central role in early detection and initiation of appropriate …

Fine-Scale Population Structure Of Japanese Helicobacter Pylori Provides New Anthropological And Epidemiological Insights, Kohei Tomonari, Batsaikhan Saruuljavkhlan, Ricky Indra Alfaray, Kartika Afrida Fauzia, Osamu Matsunari, Daisuke Chinda, Tadashi Shimoyama, Phawinee Subsomwong, Nagisa Kinjo, Tetsu Kinjo, Yasuo Hanamure, Masato Shinzato, Rumiko Suzuki, Fukunori Kinjo, Shigeru Takigami, Toshio Fujioka, Takeshi Matsuhisa, Junko Akada, Takashi Matsumoto, Yoshio Yamaoka

Faculty, Staff and Students Publications

Helicobacter pylori is considered to contribute to gastric cancer and is also used as a marker to trace human migration due to its co-evolution with humans. To understand the recently proposed tripartite model suggesting three ancestral origins for the Japanese population and address the enigma of the high incidence of gastric cancer in Northeast Hondo (Hondo is mainland Japan), we conducted a fine-scale population structure analysis using a large Japanese H. pylori dataset, including 438 strains from 9 regions based on whole-genome sequences. As a result of fineSTRUCTURE analysis, it was found that H. pylori in Northeast Hondo is genetically …

Buddy Team 9: The Legacies Of Hiv/Aids Caregiving (New York City, 1990-1993), Jeff Donaldson-Forbes

University Honors Theses

An autoethnographic thesis asks, How were the volunteers of Gay Men's Health Crisis (GMHC) Buddy Team 9 (NYC, circa 1990-1993) impacted by HIV/AIDS caregiving? The author, a former member of GMHC Buddy Team 9, provides context to the HIV/AIDS crisis in a particular era (1990-1993), place (New York City), and cultural setting (via volunteer work supporting a major AIDS service organization).

Plasma Microbial Cell-Free Dna Sequencing For The Detection Of Kingella Kingae Pediatric Spinal Infections, Asim A Ahmed, Sarah Y Park, Matthew J Smollin, Martin S Lindner, Adriana Sarmiento Clemente, Ana Del Valle Penella, P Marcelo Laufer, Carolina Sanchez-Vegas, Manuel R Cotilla, Marian E Melish, Connie Trieu, Nazia A Kabani, Joshua Cooper, Audrey R Lloyd, David W Kimberlin, John C Arnold, Alejandro Jordan-Villegas, Lori E Patterson, Catherine E Foster, Pablo Yagupsky

Faculty, Staff and Students Publications

Background: Diagnosis of Kingella kingae skeletal system infections is made challenging by the microbe's fastidious nature. Detection and quantification of circulating microbial cell-free DNA (mcfDNA) in plasma by the Karius Test, a commercial metagenomic sequencing test, may offer promise in diagnosing pediatric spinal infections caused by difficult-to-culture organisms such as K. kingae .

Methods: Plasma mcfDNA sequencing detections of K. kingae from April 2018 to December 2020 were reviewed to identify pediatric (age < 18 years) patients. Medical charts of those with spinal infections were reviewed, and mcfDNA sequencing diagnostic performance was compared with usual care tests (ie, cultures, polymerase chain reaction).

Results: Ten children with K. kingae spinal infections were identified across 7 institutions. The median age was 16.5 months (range 11-23 months). All case-patients had vertebral osteomyelitis …

Airway Immune Signatures In Severe And Fatal Infection With Covid-19, Naresh Doni Jayavelu, Jing Jing Qi, Slim Fourati, Farrah Kheradmand, Charles R Langelier, Lauren I R Ehrlich, Joann Diray-Arce, Annmarie Hoch, Monica Kraft, Patrice M Becker, Matthew C Altman, Ruth R Montgomery

Faculty, Staff and Students Publications

No abstract provided.

Performance Of Stool-Based Molecular Tests And Processing Methods For Paediatric Tuberculosis Diagnosis: A Systematic Review And Meta-Analysis, Lucía Carratalà-Castro, Shilzia Munguambe, Belén Saavedra-Cervera, Petra De Haas, Alexander Kay, Olivier Marcy, Pamela Nabeta, Willy Ssengooba, Elisabetta Ghimenton-Walters, Sozinho Acácio, Maryline Bonnet, Joanna Ehrlich, Andrew R Dinardo, Anca Vasiliu, Christoph Lange, Sabine Hermans, Anna M Mandalakas, Elisa López-Varela, Alberto L García-Basteiro

Faculty, Staff and Students Publications

Background: There has been a global pursuit to improve the diagnosis of tuberculosis in young children by applying diagnostic methods on accessible biospecimens such as stool. We aimed to conduct a systematic review on the accuracy of stool-based molecular tests for tuberculosis diagnosis in children and to assess the impact of the available pre-processing methods and other design characteristics.

Methods: In this systematic review and meta-analysis, we evaluated studies in children younger than 16 years with presumptive tuberculosis that were published in English, Spanish, French, and Portuguese from Jan 1, 2000, to May 3, 2024, in MEDLINE, Embase, and Embase …

Dosing Recommendations For Ampicillin And Ceftriaxone In The Treatment Of Pediatric Community-Acquired Pneumonia Using Monte Carlo- And Physiologic-Based Pharmacokinetic Simulations, Norint Tung, Dustin Huynh, Quang Dam, Tri Tran, Kristina G Hulten, Christopher J Harrison, Sheldon L Kaplan, Tyler H Do, Amartya Setty, Lana Hoang, John S Bradley, Jennifer Le

Faculty, Staff and Students Publications

Objective: Since 2011, Ampicillin (AMP) has been recommended as the parenteral antibiotic of choice for pediatric community-acquired pneumonia (CAP), but ceftriaxone (CRO) is recommended for unvaccinated children and those with complicated CAP. Using penicillin and CRO susceptibility data for pneumococcus, we evaluated the adequacy of currently recommended doses of AMP and CRO.

Methods: With nonlinear mixed-effects modeling v7.3, Monte Carlo simulations (MCS, N = 10,000) for AMP and CRO were conducted for 6 virtual patients aged 3 months, 1, 2, 5, 10, and 15 years. PK-Sim v9.0 was used to develop physiologic-based pharmacokinetic (PBPK) models for AMP (N = 4000) …

The Impact Of Nicu Death On Bereaved Mothers, Jennie Terranova

University Honors Theses

Infant loss in the United States occurs 5.6 per 1,000 live births. This paper examines risk factors that contribute to neonatal death. Prematurity is a risk factor for NICU admission. A portion of premature babies receiving care in the NICU will die. The stressful nature of the NICU has been studied widely in journals. The impact of NICU death on hospital staff and bereaved parents has been studied less. Mortality studies suggest that bereaved mothers are at a high risk of dying within the first 5 years after their child has died. Despite the frequency of maternal death post-child loss, …

Exploring Vaccine Efficacy And Safety For Ms Patients On Immunosuppressant Treatment In Light Of The Covid-19 Pandemic, Rain Pross

University Honors Theses

Multiple sclerosis (MS) is a chronic neurodegenerative autoimmune disorder affecting approximately 2.8 million people worldwide. Despite decades of research to understand the molecular mechanisms of MS pathology, little is known about why the neurodegeneration caused by this illness occurs. For this reason, current treatments for MS target two broader aspects of the disease: relapse management and prevention via disease-modifying therapies. These treatments often involve immunosuppressive mechanisms, creating vulnerability to infectious diseases that became particularly concerning during the COVID-19 pandemic. This literature review examines the safety and efficacy of vaccination for MS patients on immunosuppressant therapies, highlighting the novel mRNA vaccines …

40 Years After The Discovery Of Helicobacter Pylori: Performing Optimized “Subtraction” For Clinical Eradication, Yi Hu, Ren-Chun Du, Yong-Kang Lai, Yu-Xin Hu, Yu-Chen Zhu, Yan-An Zhou, Chun-Xi Shu, Bo Zhou, Li-Xiang Ling, Xu Shu, Yong Xie, James Y W Lau, Yin Zhu, David Y Graham, Nong-Hua Lu

Faculty, Staff and Students Publications

It has been 40 years since the first discovery of the important human pathogen, Helicobacter pylori (H. pylori). Eradication of the infection continues to pose significant challenges in part because of the global increase in antibiotic resistance. Generally, the “addition” strategy has failed to deliver the desired results for more than a short period and often contributed to the increase in the prevalence of resistant strains. As such, optimization of each component of the treatment strategy has become an increasingly critical focus of research. In this study, we summarized the evolution of H. pylori eradication regimens and the …

Spatiotemporal Association Of Coronavirus Disease 2019 Cases And Deaths With Exposure To Wildfire Particulate Matter In 2020, Thomas C Mchale, David R Boulware, Kelly Searle, Leda Kobziar, Phinehas Lampman, Julio C Zuniga-Moya, Ben Papadopoulos, Andrej Spec, Naomi E Hauser, George R Thompson

2020-Current year OA Pubs

BACKGROUND: Climate change is anticipated to have profound effects on human health, including in infectious diseases. Wildfires have been increasing in frequency and intensity due to climate change and have been linked to worsening respiratory disease outcomes. We aimed to demonstrate whether there was an association between wildfire smoke and coronavirus disease 2019 (COVID-19) in California during 2020.

METHODS: We used an ecologic cohort study with a spatial autoregressive model to test for associations between wildfire smoke, measured as particulate matter < 2.5 µg/m

RESULTS: In an adjusted analysis, we found a 1-month lag increase of 203 COVID-19 cases per 10 000 persons …

Aga Khan University Medical Centre

University press releases and calendars

No abstract provided.

A Flea-Borne Mystery: Unraveling Murine Typhus In A Patient With Unexplained Encephalopathy, Jose J. Loayza Pintado, Jorge A. Aboytes, Cesar Uribe

School of Medicine Publications

Murine typhus is a flea-borne rickettsial infection caused by Rickettsia typhi, commonly seen in endemic regions like Southern California and Texas. While it typically presents with fever, rash, and headache, neurological symptoms such as altered mental status are rare. We present a case of a 66-year-old male in southern Texas with alcohol use disorder who developed progressive confusion, decreased appetite, and subjective fevers. He was found to have severe hyponatremia, acute kidney injury, and atrial fibrillation with rapid ventricular response. Despite supportive care and empiric antibiotics for a urinary tract infection, his encephalopathy persisted. Further history revealed exposure to …

Analytical Approaches For Identification Of Essential Genomes Of Plasmodium Knowlesi And Babesia Divergens, Sida Ye

Graduate Doctoral Dissertations

Apicomplexa constitute a large phylum of single-celled, obligate intracellular protozoan parasites. Notably, Plasmodium spp. and Babesia spp. are apicomplexan parasites that infect red blood cells. Plasmodium species are the causative agent of malaria and are transmitted by Anopheles mosquitoes, affecting large human populations, whereas Babesia spp., transmitted through the bite of Ixodes ticks cause babesiosis.

In this dissertation, we investigate the essential genome of these parasites using high-throughput transposon mutagenesis. Identifying the essential genome is key to finding new drug targets and understanding resistance mechanisms, a crucial pursuit given the rising resistance to frontline antimalarial drugs and the challenges …

The House At The End Of Time, Gyles Jose Antonio Ferrao

Masters Theses

This thesis investigates the architectural potential of thresholds as spaces of psychological and metaphysical transformation, positioning the edge not as a boundary but as a generative spatial condition. Drawing upon Carl Jung’s conception of dream architecture as a symbolic map of the self, the project proposes a nonlinear exploration of architecture through layered references that operate across metaphysical scales. The house becomes a medium through which the liminal is made tangible, a temporal and spatial construct that blurs the binary oppositions of life and death, memory and forgetting, reason and absurdity, consciousness and the unconscious.

The project conceptualizes architecture as …

Tubercular Abscess Of Anterior Chest Wall: A Case Report, Himanshu Binji, Kale Ajay P, Akshay Kumar

Indian Journal of Health Sciences and Biomedical Research KLEU

Diagnosing musculoskeletal tuberculosis is challenging for healthcare providers and requires a high degree of suspicion. The diagnosis is strongly suggested by a combination of gradual symptom onset, clinical signs, histological or cytological characteristics, and relevant radiographic evidence. Timely diagnosis and treatment are essential to avoid significant damage to bones and joints. Tuberculosis affecting the ribs, presenting as chest swelling, is an exceptionally rare condition, with only a limited number of cases documented in medical literature. We report a case of a 65-year-old male with tuberculous involvement of the costochondral junction, presenting as a swelling in the anterior chest wall which …

A Survey On Resources, Services And Outputs At Piloted Model And Conventional Urban-Primary Health Centres In North 24 Parganas District Of West Bengal, Baisakhi Maji, Nabanita Bhattacharyya, Ankita Mishra

Indian Journal of Health Sciences and Biomedical Research KLEU

Context: Urban Primary Health Centres are being upgraded with a vision to deliver “Comprehensive Primary Health Care (CPHC)”. In 2023, the Government of West Bengal also strived to uplift the quality of services provided through UPHCs by rolling out “Piloting of model UPHC and U-HWC”.

Aims: To compare the available resources, services and outputs at Piloted Model and Conventional Urban-Primary Health Centres in North 24 Parganas District of West Bengal.

Settings and Design: An analytical study with cross-sectional design was conducted in a Piloted Model and a Conventional U-PHCs in the North 24 Parganas District of West Bengal.

Methods and …

Epidemiological Status And Diagnosis Of Leptospirosis —A Review, Pooja Palla, Gudikandula Krishna, Dasari Thrimothi

Maaen Journal for Medical Sciences

Numerous investigations carried out in impacted countries have enhanced our knowledge of the leptospirosis epidemiology, a serious zoonotic illness that has spread throughout the world. Reliable global incidence numbers are unavailable, and the infection is underreported. According to a thorough analysis and modelling exercise, there are over a million human cases and about 60,000 fatalities worldwide each year. Although temperate countries can also experience it, tropical locations are where it is most common. Sub-Saharan Africa, the Caribbean, Oceania, South and Southeast Asia, and sections of Latin America have the highest infection rates. Even though leptospirosis is a tropical zoonotic disease …

Seroprevalence And Associated Risk Factors Of Caprine Arthritis Encephalitis (Cae) And Peste Des Petits Ruminants (Ppr) Among Deer In An Institutional Farm In Malaysia, Krishnan Nair Balakrishnan, Faez Firdaus Abdullah Jesse, Bura Thlama Paul, Shalonagiftsal Stephen C Charles, Ummul Rifhan Najiha Noor Zaman, Eric Lim Teik Chung, Nur Amira Azhar, Abdirahman Barre, Muhammed Mikail, Mohd Azmi Mohd Lila

The Thai Journal of Veterinary Medicine

Caprine arthritis encephalitis (CAE) and peste des petits ruminants (PPR) are significant viral diseases impacting small ruminants, leading to substantial economic losses and posing a potential threat to livestock health. Although seroprevalence evidence of these viruses has been documented in ruminants, there is a lack of data regarding their presence among farmed deer in Malaysia. This preliminary study aimed to determine the seroprevalence and associated risk factors for CAEV and PPRV infections among deer in Malaysia. A total of ninety-two blood sera from an institutional deer farm were screened for CAEV and PPRV antibodies using commercially available indirect ELISA test …

A Century Of Innovation: The Ada And The Forsyth Institute Partnership, Manish Chopra Dmd

Metro Denver Dental Society Articulator Magazine

This article details the new partnership between the Forsyth Institute, a century-old leader in dental and craniofacial research, and the American Dental Association to create the ADA Forsyth Institute (AFI). This global research powerhouse is pioneering breakthroughs in oral microbiology, fluoride research, saliva diagnostics and AI-driven smart fillings. The partnership represents a strategic alliance aimed at elevating dental science, education and professional standards.

Job Satisfaction Among Health Workers At Primary Health Care In Palembang City, Indonesia, Asmaripa Ainy, Merri Nurmala Sarri, Poppy Fujiyanti, Hartati Inaku, Tannia Tembo

Kesmas

Health workers at primary health care are the frontline providers of community health services. This study aimed to investigate the level of job satisfaction of health workers at primary health care in Palembang City, South Sumatra Province, Indonesia, and its influencing factors. Using a questionnaire developed by the authors' team, the study involved 257 samples. A total of 66.15% of respondents indicated high satisfaction, 21.79% reported moderate satisfaction, and 12.06% expressed dissatisfaction. A statistically significant correlation was found between knowledge, skills, income, promotion opportunities, work supervision, and relationships with colleagues and job satisfaction (p-value <0.001). The correlations were strong, with Spearman's rho of 0.822, 0.869, 0.912, 0.895, and 0.821, respectively. Statistical analysis of the Wilcoxon rank-sum and Kruskal-Wallis tests showed no significant differences in median satisfaction scores between sociodemographic groups (p-value 0.072 to 0.552>0.05). These findings showed that job …

Assessing The Impact Of The First Feeding Practice On Wasting Risk Among Indonesian Children Under The Age Of Five, Azrimaidaliza Azrimaidaliza, Annisa Dwi Apriliani, Mutiara Tirta Prabandari Lintang Kusuma

Kesmas

The problem of undernutrition among children under the age of five (the under-five) continues to be alarmingly high in Indonesia, including in West Sumatra Province. Various factors contribute to this issue, with studies suggesting a relationship between intergenerational causes and undernutrition. This study analyzed the main risk factors of wasting among children in Padang City. Data were collected from 174 under-five and divided into unmatched cases that were selected randomly and control groups. The mothers were interviewed using a questionnaire regarding their children’s feeding habits, and weight and height data were collected from primary health care. Multiple logistic regression analysis …

Articulator Q2 2025

Metro Denver Dental Society Articulator Magazine

The 2^nd Quarter 2025 issue focuses on new frontiers in dental research. Articles include:

• A feature from Dr. Manish Chopra on the ADA Forsyth Institute.
• Highlights from the University of Colorado’s School of Dental Medicine’s Research Day.
• The research behind the safety of community water fluoridation from Margherita Fontana, DDS, PhD; Carlow Gonzalez-Cabezas, DDS, MSD, PhD; and Livia Tenuta, DDS, MSc, PhD.

Plus, updates on local nonprofits, a reflection on leading with integrity and tips on maintain resilience in today’s volatile financial landscape.

Carbapenem-Resistant Enterobacterales Peri-Rectal Colonization Prevalence On Admission To Two Intensive Care Units In An Academic Hospital In India, Armaghan-E-Rehman Mansoor, Jacaranda Van Rheenen, Ige A George, Jennie H Kwon, Et Al.

2020-Current year OA Pubs

This study from a South Indian tertiary care hospital found a 41% peri-rectal Carbapenem-resistant

Expansion Of A Rapid Reentry Program To Improve Viral Suppression And Retention In Hiv Care, Danielle L. Gilbert Do, Mph, Shafay Shams Md, John G. Phillips Md, Paula S. Seal Md, Mph

Quality Improvement & Patient Safety Forum

No abstract provided.

From Knowledge To Action: Assessing The Influence Of Provider Education On Vaccine Importance, Tat W. Yau Md, Mph, Lauren E. Richey Md, Mph

Quality Improvement & Patient Safety Forum

No abstract provided.

2025 Qi Forum Agenda, Equip Program Office Ogme

Quality Improvement & Patient Safety Forum

No abstract provided.

Russell’S Viper Envenomation: The Challenge Of Diagnosis, Jing-Hua Lin, Wang-Chou Sung, Jiunn-Wang Liao, Dong-Zong Hung

BioMedicine

Russell’s viper envenomation is rare in Taiwan, and its typical clinical presentations, including consumption coagulopathy, acute renal failure, haemolysis, and increased capillary permeability, have been reported in the literature as case reports or series. Here, we report a case with an atypical presentation, and suspected to be a Russell’s viper bite due to the distinct characteristics of the snake and some progressive clinical signs/symptoms. He returned to health successfully after the correct antivenom was administered, and envenomation was ultimately confirmed by venom detection in the patient’s serum and urine samples.

Nutraceutical Benefits And Neuro-Protective Potent Of Four Colored Peppers (Capsicum Annuum Var. Grossum), Ya-Chen Yang, Chih-Lung Lin, Mei-Chin Yin

BioMedicine

Background: Four colored peppers (Capsicum annuum var. grossum), orange, purple, yellow and red, are plant foods served as salad or stir-fry for meals in Taiwan and many countries.

Purpose: This study aimed to investigate the multiple nutraceutical properties of aqueous extracts prepared from colored peppers.

Methods: Vitamin C content and phytochemical profiles of these peppers were analyzed. In vitro effects of anti-oxidative, anti-a-amylase, anti-a-glucosidase, antilipase and anti-acetylcholinesterase (AchE) of pepper aqueous extracts at 0.25, 0.5 and 1 mg were evaluated. The neuronal protective potent of pepper aqueous extracts at 0.5 and 1 mg in high glucose treated nerve growth factor …