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Targeting Immunosuppressive Ly6c+ Classical Monocytes Reverses Anti-Pd-1/Ctla-4 Immunotherapy Resistance, B Leticia Rodriguez, Limo Chen, Yanli Li, Shucheng Miao, David H Peng, Jared J Fradette, Lixia Diao, Jessica M Konen, Frank R Rojas Alvarez, Luisa M Solis, Xiaohui Yi, Aparna Padhye, Laura A Gibson, Joshua K Ochieng, Xiaofei Zhou, Jing Wang, Don L Gibbons

Faculty, Staff and Student Publications

Introduction: Despite significant clinical advancement with the use of immune checkpoint blockade (ICB) in non-small cell lung cancer (NSCLC) there are still a major subset of patients that develop adaptive/acquired resistance. Understanding resistance mechanisms to ICB is critical to developing new therapeutic strategies and improving patient survival. The dynamic nature of the tumor microenvironment and the mutational load driving tumor immunogenicity limit the efficacy to ICB. Recent studies indicate that myeloid cells are drivers of ICB resistance. In this study we sought to understand which immune cells were contributing to resistance and if we could modify them in a way …

Silver(I) 1,10-Phenanthroline Complexes Are Active Against Fonsecaea Pedrosoi Viability And Negatively Modulate Its Potential Virulence Attributes, Ingrid S. Sousa Ingrid S. Sousa, Tatiana D.P. Vieira, Rubem F. S. Menna-Barreto, Allan J. Guimarães, Pauraic Mccarron, Malachy Mccann, Michael Devereux, André L. S. Santos, Lucimar F. Kneipp

Articles

The genus Fonsecaea is one of the etiological agents of chromoblastomycosis (CBM), a chronic subcutaneous disease that is difficult to treat. This work aimed to evaluate the effects of copper(II), manganese(II) and silver(I) complexes coordinated with 1,10-phenanthroline (phen)/1,10- phenanthroline-5,6-dione (phendione) on Fonsecaea spp. Our results revealed that most of these complexes were able to inhibit F. pedrosoi, F. monophora and F. nubica conidial viability with minimum inhibitory concentration (MIC) values ranging from 0.6 to 100 M. The most effective complexes against F. pedrosoi planktonic conidial cells, the main etiologic agent of CBM, were [Ag(phen)2]ClO4 and [Ag2(3,6,9-tdda)(phen)4].EtOH, (tdda: 3,6,9-trioxaundecanedioate), displaying MIC …

Silver(I) And Copper(Ii) 1,10-Phenanthroline-5,6-Dione Complexes As Promising Antivirulence Strategy Against Leishmania: Focus On Gp63 (Leishmanolysin), Simone S.C. Oliveira, Claudyane A. Correia, Vanessa S. Santos, Elaine F.F. Da Cunha, Alexandre A. De Castro, Teodorico C. Ramalho, Michael Devereux, Malachy Mccann, Marta H. Branquinha, Andre L.S. Santos

Articles

Leishmaniasis, caused by protozoa of the genus Leishmania, encompasses a group of neglected diseases with diverse clinical and epidemiological manifestations that can be fatal if not adequately and promptly managed/treated. The current chemotherapy options for this disease are expensive, require invasive administration and often lead to severe side effects. In this regard, our research group has previously reported the potent anti-Leishmania activity of two coordination compounds (complexes) derived from 1,10-phenanthroline-5,6-dione (phendione): [Cu(phendione)3 ].(ClO4 )2 .4H2O and [Ag(phendione)2 ].ClO4 . The present study aimed to evaluate the effects of these complexes on leishmanolysin (gp63), a virulence factor produced by all Leishmania …

Peptidases Are Potential Targets Of Copper(Ii)-1,10-Phenanthroline-5,6-Dione Complex, A Promising And Potent New Drug Against Trichomonas Vaginalis, Graziela Vargas Rigo, Fernanda Gomes Cardoso, Matheus Mendonca Pereira, Michael Devereux, Malachy Mccann, Andre L.S. Santos, Tiana Tasca

Articles

Trichomonas vaginalis is responsible for 156 million new cases per year worldwide. When present asymptomatically, the parasite can lead to serious complications, such as development of cervical and prostate cancer. As infection increases the acquisition and transmission of HIV, the control of trichomoniasis represents an important niche for the discovery and development of new antiparasitic molecules. This urogenital parasite synthesizes several molecules that allow the establishment and pathogenesis of infection. Among them, peptidases occupy key roles as virulence factors, and the inhibition of these enzymes has become an important mechanism for modulating pathogenesis. Based on these premises, our group recently …

Parents' Experiences Of Infant And Young Child Feeding During The Covid-19 Pandemic In Ireland, Elizabeth J. O'Sullivan, Aileen Kennedy

Articles

Objective: The WHO has urged member states to develop preparedness plans for infant and young child feeding (IYCF) during emergencies. Ireland has no such plan. We aimed to identify the needs of caregivers in Ireland with regards IYCF during the COVID-19 pandemic. Design: Online survey conducted in May-June 2020. Setting: Ireland, during the first period of severely restricted movement due to COVID-19 (lockdown). Participants: Respondents (n 745) were primary caregivers of a child under 2 years; they were primarily well educated and likely of higher socio-economic status. Results: Among those who breastfed, being unable to access breast-feeding support groups and …

A Randomized Controlled Trial Of An Oral Probiotic To Reduce Antepartum Group B Streptococcus Colonization And Gastrointestinal Symptoms, Lisa Hanson, Leona Vandevusse, Marie Forgie, Emily Malloy, Maharaj Singh, Maryanne Scherer, Diana Kleber, Jonah Dixon, Andrew J. Hryckowian, Nasia Safdar

College of Nursing Faculty Research and Publications

BACKGROUND

Probiotics have been suggested as a strategy to reduce antenatal group B Streptococcus colonization. Although probiotics are known to improve gastrointestinal symptoms, this has not been studied during pregnancy.

OBJECTIVE

This study aimed to evaluate the efficacy of a probiotic to reduce: (1) standard-of-care antenatal group B Streptococcus colonization and colony counts and (2) gastrointestinal symptoms of pregnancy.

STUDY DESIGN

In a double-blind fashion, 109 healthy adult pregnant people were randomized to Florajen3 probiotic or placebo capsules once daily from 28 weeks’ gestation until labor onset. Baseline vaginal and rectal study swabs for group B Streptococcus colony-forming units and …

The Monroe Method: A Methodology On Navigating Race, Oppression, And Equity In Medical Education Through Physician Cultural Responsibility, Christen D. Johnson

Antioch University Dissertations & Theses

Many forms of oppression create barriers for health care, further health disparities, and impact the wellness of physicians. As health disparities, caused by the social determinants of health, complicate the practice of medicine, physicians’ risk of burnout increases. The practice of Physician Cultural Responsibility provides a means to overcome health disparities and support physicians while embracing the intersectionality of the populations they serve. Incorporation of Physician Cultural Responsibility into physician professional identity is essential for the practice to be life-long. As there is no standardized curriculum to address teaching the practice of Physician Cultural Responsibility, this study aims to evaluate …

Clinical Manifestations And Outcome Of Patients With Primary Amoebic Meningoencephalitis In Pakistan A Single-Center Experience, Shakeel Ur Rehman, Salman Farooq, Muhammad Bilal Tariq, Nosheen Nasir, Mohammad Wasay, Sobia Masood, Musa Karim

Faculty, Staff and Student Publications

Primary amoebic meningoencephalitis (PAM) is a rapidly progressing central nervous system (CNS) infection caused by Naegleria fowleri, a free-living amoeba found in warm freshwater. The disease progression is very rapid, and the outcome is nearly always fatal. We aim to describe the disease course in patients admitted with PAM in a tertiary care center in Karachi, Pakistan between the periods of 2010 to 2021. A total of 39 patients were included in the study, 33 males (84.6%). The median age of the patients was 34 years. The most frequent presenting complaint was fever, which was found in 37 patients (94.9%) …

Perceptions Of Florida Victim Advocates During The Covid-19 Pandemic, Katarina Renee Hamburg

Theses and Dissertations

In December of 2019, a new virus known as COVID-19 emerged out of Wuhan, China. COVID-19 is a respiratory virus which is highly contagious and, in some cases, lethal. By January 20th, 2020, the United States reported its first case of COVID-19. Between January and December of 2020 there were 18.7 million cases and 329,000 deaths in the United States alone. Globally, during that time frame, there were 79.8 million cases and 1.75 million deaths. Due to the highly contagious and dangerous nature of COVID-19, countries across the world have attempted to promote public health by enacting social distancing measures. …

Human Trafficking: The Impact Of Covid-19 On Labor Trafficking In The Us, Anthony A. Mottola

Theses and Dissertations

Human trafficking is a global phenomenon where traffickers prey upon their victims without discriminating against age, gender, ethnic background, or nationality. The United States (US) is not immune to the human rights violations of human trafficking. The victims of human trafficking are coerced into a life of exploitation through forced labor or sexual exploitation. Many victims are migrants from disadvantaged countries that travel to the US seeking employment but end up the victims of abuse, both physically and mentally, at the hands of traffickers. In 2020, the COVID-19 pandemic devastated the global economy, forcing quarantines, travel bans, and social distancing. …

A Mixed-Method Study On Social Media’S Role In The Health Decision-Making Practices Of Black Millennial Women In South Florida, Sashah Damier

Theses and Dissertations

The purpose of this research study was to examine health care decision-making practices of Black millennial women in South Florida, based on their social media use. The study contributed to understanding of how cultural components contribute to health care disparities for people of color because health care providers lacked information on how to reach Black millennial women with accurate and helpful information through social media.

The design for the study included a convergent parallel mixed-methods design using a web-based quantitative cross-sectional survey along with embedded qualitative telephone interviews and NVivo software. This mixed-methods methodology provided rich data and new understandings …

Teachers’ Experiences In Implementing Primary School Physical Education Curriculum In Ghana And The United States, Patrick Collins Frempong

Theses and Dissertations

Barriers to implementing a full physical education curriculum presented challenges to teaching students about healthy lifestyles and supporting them through effective lessons. This mixed-methods study aimed to explore and compare primary school teachers’ experiences in rural Ghana and the United States in implementing the physical education curriculum. The present study addresses gaps in the literature by connecting teacher concerns when implementing a curriculum with fidelity. The mixed-methods study helped explore and compare primary school teachers’ experiences in rural (town populations of less than 5,000) Ghana and rural towns of the southern United States in implementing the physical education curriculum.

This …

We Can Do This / Juntos Sí Podemos: A Critical Discourse Analysis Of Government-Sponsored Covid-19 Public Service Announcements (Psas) In English And Spanish, Katalina R. Peterson

Scripps Senior Theses

Communication inequities are known to negatively impact people from socioeconomically and linguistically disadvantaged backgrounds during public health crises (Gomez-Aguinaga et al., 2021). In the United States, Hispanics—especially those who speak Spanish—have been among the communities most disproportionately affected by COVID-19 (National Center for Health Statistics, 2022). The pandemic has underscored the importance of understanding the linguistic and discursive strategies implemented by institutions entrusted with disseminating public health information to reach diverse audiences, especially the most vulnerable. This paper analyzes the linguistic practices used to create public service announcements (PSA) produced in English and Spanish by health agencies at both the …

From Smallpox To Covid-19: Social And Legal Responses To Vaccinations And Vaccine Mandates, Janet L. Dolgin

Hofstra Law Faculty Scholarship

No abstract provided.

Identifying Host-Intrinsic Resistance Mechanisms To Therapeutic Anti-Tumor Antibody, James Edward Glassbrook

Wayne State University Dissertations

The use of monocloncal antibodies (mAbs) directed at tumor-associated antigens (TAA), often termed “targeted immunotherapy” has revolutionized cancer treatment, markedly improving patient outcomes. As a prime example, the mAb trastuzumab targets human epidermal growth factor 2 (HER2). Like many targeted immunotherapies, only a subset of patients with HER2+ cancer exhibit long-term benefit from trastuzumab. In addition to tumor-intrinsic factors, such as HER2 heterogeneity, we hypothesize that host genetics may influence subsequent immune activation after treatment. To model this interaction, we utilized the genetically heterogeneous Diversity Outbred (DO) mouse model in an F1 cross with BALB/c (producing DOCF1 mice). This cohort …

Power And Sample Size Calculations For Evaluating Spillover Effects In Networks With Non-Randomized Interventions, Ke Zhang

Open Access Master's Theses

Determining the appropriate sample size that is needed to achieve a desired level of statistical power is an integral aspect of study design, as it ensures that studies are adequately powered and that their results are reliable and meaningful. Adequate statistical power can also address some practical and ethical considerations. Various methodologies for power and sample size calculations have been developed for distinct types of studies. However, the implications for power when estimating spillover effects in sociometric network-based studies with non-randomized interventions (SNS-NRIs) remain inadequately explored. In this study, we first conducted a series of simulations to evaluate how the …

Recovering The Full Network Using A Sampling Bias Adjustment In Sampled Network, Ryan Humphreys

Open Access Master's Theses

This project aimed to investigate the differences in terms of network structural characteristics between full and sampled networks and to develop a new weighted generalized estimating equations model (WGEE) in order to mitigate the sampling bias and obtain less biased sample estimates for model coefficients and standard errors. The applications of such an approach are of interest in the field of network analysis and public health studies. The Transmission Reduction Intervention Project (TRIP) is an HIV study using risk networks and is a key motivator for this work. Two other motivating examples were constructed where it was found that sampled …

Historical And Technical Analysis Of A Baltimore Album Quilt Top, Susan E. Day

Open Access Master's Theses

Almost 200 years after their creation, quilts in the Baltimore Album style continue to fascinate scholars and collectors. They encompass the most intricate of appliqué designs accomplished with sophisticated skills. Their designers remain little-known. A previously unresearched and unfinished Baltimore Album Quilt top is described and analyzed. It is compared to three other historic Baltimore Album Quilts from museum collections, all inscribed and dated between 1845 and 1850, all originating from northern Baltimore County, Maryland, and all interrelated through designs or inscriptions.

Research questions address the maker(s) of the top, the identity and circumstances of the intended recipient, and the …

Selection And Characterization Of Bacteriocinogenic Lactic Acid Bacteria From The Intestine Of Gilthead Seabream (Sparus Aurata) And Whiting Fish (Merlangius Merlangus): Promising Strains For Aquaculture Probiotic And Food Bio-Preservation, Sarah Cheriet, Sana Lenglitz, Amel Romdhani, Paul Hynds, Mohamed Salah Abbassi, Taofik Ghrairi

Articles

This study sought to evaluate the probiotic properties and the food preservation ability of lactic acid bacteria isolates collected from the intestines of wild marine fishes (gilthead seabream (Sparus aurata) (n = 60) and whiting fish (Merlangius merlangus) (n = 40)) from the Mediterranean sea in the area of Mostaganem city, Algeria. Forty-two isolates were identified as: Enterococcus durans (n = 19), Enterococcus faecium (n = 15), Enterococcus faecalis (n = 4), Lactococcus lactis subp. lactis (n = 3), and Lactobacillus plantarum (n = 1). All isolates showed inhibition to at least one indicator strain, especially against Listeria monocytogenes, Staphylococcus …

An Ecological Study Of The Spatiotemporal Dynamics And Drivers Of Domestically Acquired Campylobacteriosis In Ireland, 2011–2018, Martin Boudou, Coilín Ó Haiseadha, Patricia Garvey, Jean O'Dwyer, Paul Hynds

Articles

In 2021, Campylobacteriosis was the main gastrointestinal disease in the European Union since 2007 according to the European Centre for Disease Prevention and Control. In the Republic of Ireland, the incidence of the disease is particularly high with approximately 3,000 cases per annum, raising significant concerns for national health authorities with an expected increase in the number of cases in the light of climate change. The current study sought to assess the spatio-temporal patterns of campylobacteriosis in the Republic of Ireland using 20,391 cases from January 2011 to December 2018. An ensemble of spatial statistics techniques including seasonal decomposition, spatial …

Medical Diagnostic Value Of Digital Pcr (Dpcr): A Systematic Review, Sophia Nazir

School of Engineering, Computing and Mathematics

Digital polymerase chain reaction (dPCR) is an emerging technique for the absolute quantification of target nucleic acids. dPCR got attention as a precise quantification tool in preclinical research, particularly when used to detect genetic mutations and result in highly precise measurements. In dPCR, the statistic of Poisson distribution was followed for the random distribution of molecules in different partitions, which is essential for dPCR quantification. Amplified target sequences in different partitions are identified by fluorescence and each partition functions as a separate PCR microreactor. Without the need for calibration, the percentage of PCR-positive partitions is sufficient to estimate the concentration …

Recent Progress Of Molecular Diagnosis Via Crispr Cas-Based Biosensors And Bioassays, S. Nazir

School of Engineering, Computing and Mathematics

The immune systems of bacteria and archaea served as the inspiration for CRISPR/Cas technology, which won the 2020 Nobel Prize in Chemistry for its achievements in genetic manipulation. Because of its enormous value globally, molecular diagnostics emerged as a fresh wave of diagnostic technologies. A growing number of studies have demonstrated the compatibility of CRISPR/Cas technology with biosensors and bioassays for molecular diagnostics. As highly precise, sensitive, easily programmable, and device-independent sensors, CRISPR-based detection has received a lot of interest. One of the most precise and sensitive diagnostic techniques is nucleic acid-based detection. It has the potential for detecting other …

Electrochemical Biosensors For The Detection Of Antibiotics In Milk: Recent Trends And Future Perspectives, Baljit Singh Dr., Abhijnan Bhat, Lesa Dutta, Kumari Riya Pati, Yaroslav Korpan, Isha Dahiya

Articles

Antibiotics have emerged as ground-breaking medications for the treatment of infectious diseases, but due to the excessive use of antibiotics, some drugs have developed resistance to microorganisms. Because of their structural complexity, most antibiotics are excreted unchanged, polluting the water, soil, and natural resources. Additionally, food items are being polluted through the widespread use of antibiotics in animal feed. The normal concentrations of antibiotics in environmental samples typically vary from ng to g/L. Antibiotic residues in excess of these values can pose major risks the development of illnesses and infections/diseases. According to estimates, 300 million people will die prematurely in …

Ocean Acidification Has A Strong Effect On Communities Living On Plastic In Mesocosms, X Zhang, P Zhang, Zichao Deng, Ruiping Huang, D Zhang, Yang Tian, N Wang, He Li, X Wang, Xiaowen Jiang, Jiazhen Sun, Qianqian Fu, Xiangqi Yi, Liming Qu, Cong Zhou, Yuming Rao, Xiaorong Zeng, Jm Hall‐Spencer, G Gao, K Gao, Xin Lin

School of Biological and Marine Sciences

We conducted a mesocosm experiment to examine how ocean acidification (OA) affects communities of prokaryotes and eukaryotes growing on single-use drinking bottles in subtropical eutrophic waters of the East China Sea. Based on 16S rDNA gene sequencing, simulated high CO2 significantly altered the prokaryotic community, with the relative abundance of the phylum Planctomycetota increasing by 49%. Under high CO2, prokaryotes in the plastisphere had enhanced nitrogen dissimilation and ureolysis, raising the possibility that OA may modify nutrient cycling in subtropical eutrophic waters. The relative abundance of pathogenic and animal parasite bacteria also increased under simulated high CO2. Our results show …

Urinary Tract Infection And Associated Factors Among Pregnant Women Receiving Antenatal Care At A Primary Health Care Facility In The Northern Region Of Ghana, Ezekiel K. Vicar, Samuel E. K. Acquah, Williams Wallana, Eugene D. Kuugbee, Emmanuel K. Osbutey, Abigail Aidoo, Emmanuel Acheampong, Gloria I. Mensah

Research outputs 2022 to 2026

Urinary tract infection (UTI) is frequently encountered during pregnancy and is associated with adverse maternal, fetal, and neonatal effects. However, very little information is available on the prevalence of UTI among pregnant women in the northern part of Ghana, a region with a high birth rate. This study employed a cross-sectional analysis of the prevalence, antimicrobial profile, and risk factors associated with UTI in 560 pregnant women attending primary care for antenatal check-ups. Sociodemographic obstetrical history and personal hygiene information were obtained using a well-structured questionnaire. Afterward, clean catch mid-stream urine samples were collected from all participants and subjected to …

Externalizing Traits: Shared Causalities For Covid-19 And Alzheimer's Dementia Using Mendelian Randomization Analysis, Haotian Wang, Mingyang Cao, Yingjun Xi, Weijie Cao, Xiaoyu Zhang, Xiaoni Meng, Deqiang Zheng, Lijuan Wu, Wei Wang, Di Liu, Youxin Wang

Research outputs 2022 to 2026

Externalizing traits have been related with the outcomes of coronavirus disease 2019 (COVID-19) and Alzheimer's dementia (AD); however, whether these associations are causal remains unknown. We used the two-sample Mendelian randomization (MR) approach with more than 200 single-nucleotide polymorphisms (SNPs) for externalizing traits to explore the causal associations of externalizing traits with the risk of COVID-19 (infected COVID-19, hospitalized COVID-19, and severe COVID-19) or AD based on the summary data. The inverse variance–weighted method (IVW) was used to estimate the main effect, followed by several sensitivity analyses. IVW analysis showed significant associations of externalizing traits with COVID-19 infection (odds ratio …

Spore-Forming Clostridium (Clostridioides) Difficile In Wastewater Treatment Plants In Western Australia, Jessica M. Chisholm, Papanin Putsathit, Thomas V. Riley, Su-Chen Lim

Research outputs 2022 to 2026

There is growing evidence that shows Clostridium (Clostridioides) difficile is a pathogen of One Health importance with a complex dissemination pathway involving animals, humans, and the environment. Thus, environmental discharge and agricultural recycling of human and animal waste have been suspected as factors behind the dissemination of Clostridium difficile in the community. Here, the presence of C. difficile in 12 wastewater treatment plants (WWTPs) in Western Australia was investigated. Overall, C. difficile was found in 90.5 % (114/126) of raw sewage influent, 48.1 % (50/104) of treated effluent, 40 % (2/5) of reclaimed irrigation water, 100 % (38/38) of untreated …

Global Uncertainty And Economic Growth–Evidence From Pandemic Periods, Deepa Bannigidadmath, M. H. A. Ridhwan, Fiskara Indawan

Research outputs 2022 to 2026

This paper investigates whether global uncertainty predicts economic growth rates using a global sample of 136 countries. We use the panel regression model and find strong evidence that global uncertainty negatively predicts the economic growth rate. Further, the negative impact of global uncertainty on economic growth rates is amplified during pandemic periods versus non-pandemic periods. Our main findings hold after a range of robustness tests.

Biofilm Formation On The Surface Of Monazite And Xenotime During Bioleaching, Arya Van Alin, Melissa K. Corbett, Homayoun Fathollahzadeh, M. Christian Tjiam, William D. A. Rickard, Xiao Sun, Andrew Putnis, Jacques Eksteen, Anna H. Kaksonen, Elizabeth Watkin

Research outputs 2022 to 2026

Microbial attachment and biofilm formation is a ubiquitous behaviour of microorganisms and is the most crucial prerequisite of contact bioleaching. Monazite and xenotime are two commercially exploitable minerals containing rare earth elements (REEs). Bioleaching using phosphate solubilizing microorganisms is a green biotechnological approach for the extraction of REEs. In this study, microbial attachment and biofilm formation of Klebsiella aerogenes ATCC 13048 on the surface of these minerals were investigated using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). In a batch culture system, K. aerogenes was able to attach and form biofilms on the surface of three phosphate …

Overview Of Aboriginal And Torres Strait Islander Health Status 2022, Australian Indigenous Healthinfonet

Research outputs 2022 to 2026

The main purpose of the Overview of Aboriginal and Torres Strait Islander health status (Overview) is to provide a comprehensive summary of the most recent indicators of the health and current health status of Australia’s Aboriginal and Torres Strait Islander people. The Overview has been prepared by HealthInfoNet staff as part of our contribution to supporting those who work in the Aboriginal and Torres Strait Islander health sector. The Overview is a key indicator of the HealthInfoNet’s commitment to authentic and engaged knowledge development and exchange. The initial sections of this Overview provide information about the context of Aboriginal and …